Composite dietary composition for improving microcirculation and preparation method thereof

Abstract

This application relates to the field of functional health foods and provides a composite dietary composition for improving microcirculation and its preparation method. This composite dietary composition for improving microcirculation includes the following components (by weight): freeze-dried fruit and vegetable composite powder, European blueberry concentrate powder, Sophora japonica rice flour, (3R,3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder, and multivitamins. This application utilizes freeze-dried fruit and vegetable composite powder, European blueberry concentrate powder, Sophora japonica rice flour, (3R,3'R)-dihydroxy-β-carotene, and adds matcha powder, multivitamins, fermented ginger powder, and lutein microcapsule powder. This composition is rich in nutrients and has the effects of regulating microcirculation, promoting capillary softening, and promoting microcirculation in the eye.

Description

Technical Field

[0001] The present application relates to the field of functional health foods, and in particular to a composite dietary composition for improving microcirculation and a preparation method thereof. Background Art

[0002] Every organ in the human body has microcirculation, and microcirculatory disturbances can manifest in a variety of symptoms. The eyes, with their rich blood vessels, are a microcosm of the body's microvasculature, reflecting its overall condition. Disruption of ocular microcirculation may result in symptoms such as dry and itchy eyes, tearing in response to wind or photophobia, soreness, swelling, or stinging in the eyes, bloodshot eyes, blurred vision, dark circles, and eye fatigue.

[0003] In the related art, some commercially available dietary products mainly add lutein esters as functional ingredients to promote and maintain good ocular microcirculation. However, the content of lutein esters in food is very low, and it does not exist in plasma or in the macular area of ​​the optic retina. Moreover, after entering the human body, lutein esters need to be decomposed and converted into free lutein before being absorbed by the human body. The deesterification requires the help of fatty acid enzymatic cleavage process, and this complex enzymatic cleavage process varies significantly among individuals, so it has a greater impact on the digestion and absorption effect. Summary of the Invention

[0004] To address the above-mentioned deficiencies in the prior art, the present application aims to provide a microcirculatory-improving composite dietary composition and its preparation method. The microcirculatory-improving composite dietary composition of the present application is formulated using a specific ratio of freeze-dried fruit and vegetable composite powder, European blueberry concentrate powder, sophora japonica rice powder, and (3R,3'R)-dihydroxy-β-carotene, along with matcha powder, multivitamins, fermented ginger powder, and lutein microcapsule powder. The composition is rich in nutrients, and proper consumption can supplement the body's essential nutrients and maintain nutritional balance. It also effectively regulates microcirculation, promotes capillary softening, and promotes ocular microcirculation.

[0005] To achieve the above-mentioned invention objectives, the technical solutions adopted in this application are as follows:

[0006] In a first aspect, the present application provides a composite dietary composition for improving microcirculation, comprising the following components:

[0007] Freeze-dried fruit and vegetable compound powder, European blueberry concentrate powder, sophora japonica rice powder, (3R,3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and multivitamins;

[0008] The lutein microcapsule powder comprises the following components: lutein, maltooligosaccharide, octenylsuccinate monoarabic gum ester, vitamin E, silicon dioxide and ascorbyl palmitate;

[0009] The preparation steps of lutein microcapsule powder are as follows:

[0010] Dissolving the composite emulsifier in water to prepare a first solution, and then adding octenyl succinate monoarabic gum ester to the first solution and mixing them to obtain a second solution;

[0011] Add ascorbyl palmitate to oil and heat to dissolve to obtain a third solution; add lutein, vitamin E, and silicon dioxide to the third solution and disperse and homogenize to obtain a fourth solution;

[0012] Splitting the second solution into a first aqueous phase and a second aqueous phase; Splitting the fourth solution into a first oil phase and a second oil phase;

[0013] adding the first water phase to the first oil phase at a first speed for shear emulsification to obtain a first emulsion;

[0014] adding the second aqueous phase to the first emulsion at a second speed for shear emulsification to obtain a second emulsion;

[0015] adding the second oil phase to the second emulsion at a third speed for shear emulsification to obtain a third emulsion; wherein the first speed is less than the third speed, and the third speed is less than the second speed;

[0016] The third emulsion is subjected to high-pressure homogenization and spray drying to obtain lutein microcapsule powder.

[0017] In a second aspect, the present application provides a method for preparing the composite dietary composition for improving microcirculation according to the first aspect, comprising:

[0018] Freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, sophora japonica rice powder, (3R, 3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and complex vitamins are mixed with a tablet flow aid, and tablets are formed to obtain the product.

[0019] The beneficial effects of this application include at least:

[0020] The composite dietary composition for improving microcirculation provided in the embodiments of the present application is formulated with freeze-dried fruit and vegetable composite powder, European blueberry concentrate powder, sophora japonica rice powder, (3R,3'R)-dihydroxy-β-carotene, and supplemented with matcha powder, multivitamins, fermented ginger powder, and lutein microcapsule powder. The composition is rich in nutrients and, when consumed in moderation, can replenish the body's essential nutrients and maintain nutritional balance. It also regulates microcirculation, promotes capillary softening, and improves ocular microcirculation. Furthermore, the lutein microcapsule powder used in the embodiments of the present application exhibits excellent stability and sustained release properties. The effective sustained-release ingredient, lutein, can be directly absorbed by the body, resulting in high bioavailability. Furthermore, by combining the lutein microcapsule powder with (3R,3'R)-dihydroxy-β-carotene in a specific ratio, the two create a synergistic effect, further enhancing the bioavailability of lutein and (3R,3'R)-dihydroxy-β-carotene. DETAILED DESCRIPTION

[0021] In order to make the technical problems, technical solutions and beneficial effects to be solved by this application more clearly understood, the following is a further detailed description of this application in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain this application, but the implementation methods of this application are not limited thereto.

[0022] Unless otherwise defined, the technical terms used in the following examples have the same meanings as commonly understood by those skilled in the art to which this application relates. The experimental reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents and are commercially available. The amounts of experimental reagents used, unless otherwise specified, are those used in routine experimental procedures. The experimental methods, unless otherwise specified, are all conventional methods.

[0023] In a first aspect, this embodiment provides a composite dietary composition for improving microcirculation, comprising the following components:

[0024] Freeze-dried fruit and vegetable compound powder, European blueberry concentrate powder, sophora japonica rice powder, (3R,3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and multivitamins;

[0025] The lutein microcapsule powder comprises the following components: lutein, maltooligosaccharide, octenylsuccinate monoarabic gum ester, vitamin E, silicon dioxide and ascorbyl palmitate;

[0026] The preparation steps of lutein microcapsule powder are as follows:

[0027] S1, dissolving a composite emulsifier in water to prepare a first solution, and then adding octenyl succinate monoarabic gum ester to the first solution and mixing them to obtain a second solution;

[0028] S2. Add ascorbyl palmitate to the oil and heat to dissolve it to obtain a third solution. Add lutein, vitamin E, and silicon dioxide to the third solution and disperse and homogenize them to obtain a fourth solution.

[0029] S3, splitting the second solution into a first aqueous phase and a second aqueous phase; splitting the fourth solution into a first oil phase and a second oil phase;

[0030] S4, adding the first aqueous phase to the first oil phase at a first speed for shear emulsification to obtain a first emulsion;

[0031] S5, adding the second aqueous phase to the first emulsion at a second speed for shear emulsification to obtain a second emulsion;

[0032] S6. Adding the second oil phase to the second emulsion at a third speed for shear emulsification to obtain a third emulsion; wherein the first speed is less than the third speed, and the third speed is less than the second speed;

[0033] S7. High-pressure homogenization and spray drying are performed on the third emulsion to obtain lutein microcapsule powder.

[0034] In the related art, most of them are lutein esters directly applied to functional foods and health foods, or lutein esters are prepared into lutein ester microcapsule powder, and then the lutein ester microcapsule powder is applied to functional foods and health foods. However, lutein ester is a fatty acid ester of lutein, which is formed by esterification reaction between lutein and fatty acids. Its content in food is very small, and it is not present in plasma and the macula of the retina. After entering the human intestine, it needs to be decomposed and converted into a free form before it can be absorbed by the human body. In this process, it needs to use the fatty acid enzymatic cleavage process to complete deesterification, and this complex process varies significantly among individuals, thus affecting the absorption effect. Therefore, when lutein ester or lutein ester microcapsule powder is applied to functional foods and health foods, the effect of human absorption varies greatly, and the human digestion and absorption process is complex and the bioavailability is low.

[0035] Lutein has a free-form structure (i.e., exists in a free state) and is abundant in foods (such as marigolds). It is present in plasma and the macula of the retina and can be directly absorbed by the human body. The present embodiment of the present invention, by preparing lutein into lutein microcapsule powder, not only reduces the variability in lutein absorption between individuals, but also releases the lutein in the microcapsule powder through a sustained-release method after entering the human body. The released lutein can be directly absorbed and utilized by the human body, simplifying the lutein absorption process and improving its bioavailability.

[0036] In some embodiments, in the above step S1, the composite emulsifier is a combination of mealworm protein, maltooligosaccharide and Tween.

[0037] As an example, mealworm protein can be produced by the following steps: dry mealworm powder at 60°C to remove moisture. The dried mealworm powder is then pulverized, passed through a 60-mesh sieve, and defatted with petroleum ether to produce fat-free mealworm powder. The fat-free mealworm powder is then mixed with a 2 mol / L NaOH solution at a volume ratio of 1:20 and stirred for 4 hours. The mixture is then centrifuged at 5000 rpm for 15 minutes, diluted with anhydrous ethanol, and allowed to stand for 2 hours. After centrifugation for 15 minutes, the supernatant is extracted and adjusted to pH 4.0 with 2 mol / L hydrochloric acid. The protein is then decontaminated with anhydrous ethanol and freeze-dried to produce mealworm protein. The resulting mealworm protein exhibits excellent water solubility.

[0038] Tween (also known as polysorbate) is a nonionic surfactant that acts synergistically with mealworm protein. During the shear emulsification process between the aqueous and oil phases, Tween rapidly migrates to the oil-water interface, reducing surface tension and enabling the rapid dispersion of oil droplets in the aqueous phase, forming an initial emulsion system. The mealworm protein then adsorbs on the surface of the oil droplets, working together with the nonionic surfactant to further reduce the interfacial energy. The mealworm protein forms an interfacial film on the oil droplet surface with a certain thickness and mechanical strength, but there may be some weak spots. Tween fills these weak areas and bonds with the mealworm protein through hydrophobic interactions and hydrogen bonds, enhancing the overall strength and stability of the interfacial film and preventing oil droplet aggregation and emulsion breakage.

[0039] Mealworm protein and malt oligosaccharides have a synergistic effect. During the shear emulsification process between the aqueous and oil phases, after the mealworm protein adsorbs on the surface of the oil droplets, the malt oligosaccharides can interact with the mealworm protein, such as through covalent bonding or physical adsorption, to form a hydration layer around the protein molecules. This increases the effective volume of the protein molecules, further enhancing the steric hindrance effect and making it more difficult for the oil droplets to approach and aggregate. Malt oligosaccharides can also regulate the conformation and stability of the mealworm protein, allowing it to better maintain its emulsifying properties under different environmental conditions (such as pH, temperature, and ionic strength). For example, under certain acidic conditions, the oligosaccharides can protect the protein structure and prevent denaturation, thereby maintaining normal adsorption and emulsification of the protein at the oil-water interface.

[0040] Tween and maltooligosaccharide have a synergistic effect. During the shear emulsification process of the water phase and the oil phase, maltooligosaccharide has a certain hydrophilicity and can interact with the hydrophilic group of Tween to adjust the hydrophilicity of the compound system, so that the compound emulsifier can better play its role in different oil-water ratios and system compositions, and improve the adaptability and versatility of the emulsifier. Secondly, maltooligosaccharide can promote the adsorption and arrangement of Tween at the oil-water interface, making the distribution of surfactant molecules on the interface more uniform, thereby more effectively reducing surface tension and improving emulsification efficiency.

[0041] By combining mealworm protein, maltooligosaccharide, and Tween to form a composite emulsifier, a denser, more stable, and elastic composite interfacial film can be formed at the oil-water interface, significantly improving emulsification ability, resulting in smaller and more uniform particle size distribution in the emulsion, and significantly enhancing the stability of the emulsion. Under different environmental conditions such as pH, temperature, and ionic strength, the mealworm protein, maltooligosaccharide, and Tween complement and synergize with each other, giving the composite emulsifier a wider range of applications and better resistance to external factors that affect emulsion stability. Furthermore, by introducing the composite emulsifier into octenylsuccinate monoarabic gum, it not only exerts excellent emulsification during the shear emulsification process between the aqueous and oil phases, but also imparts additional functionalities to the product. For example, the mealworm protein provides nutrition, the maltooligosaccharide acts as a prebiotic, and the lutein microcapsule powder is more easily absorbed by the human body, thereby improving its bioavailability. Furthermore, the amount of emulsifier used can be reduced, which helps lower production costs.

[0042] By introducing small molecule components (especially oligomaltose) into octenylsuccinate as wall materials, these small molecule components themselves have a certain emulsification stability, which can make the subsequent shear emulsification process easier and enable the shear emulsification process to be carried out at a lower temperature.

[0043] In some embodiments, in step S1 above, the mass ratio of octenyl succinate monoarabic gum ester to the composite emulsifier is 1:0.1 to 0.5. For example, the mass ratio of octenyl succinate monoarabic gum ester to the composite emulsifier can be 1:0.1, 1:0.2, 1:0.3, 1:0.4, or 1:0.5.

[0044] In some embodiments, during the steps of preparing the first, second, and third emulsions, the temperature is controlled at 25-45°C and the pH is controlled at 4-7. This allows the complex system of mealworm protein, maltooligosaccharide, and Tween to remain stable in the shear emulsification system, thereby improving the stability of the resulting emulsions.

[0045] In some embodiments, in step S2, ascorbyl palmitate may be added to deep-sea fish oil, linseed oil, or vegetable oil and heated to dissolve to obtain a third solution, and lutein, vitamin E, and silicon dioxide are then added to the third solution. The vegetable oil may be any commonly used vegetable oil in the art, specifically soybean oil, sunflower oil, peanut oil, or corn oil, or a mixture of any combination thereof in any proportion.

[0046] Ascorbyl palmitate is first dissolved in oil by heating to form a third solution, and then lutein, vitamin E and silicon dioxide are added to the third solution. The lutein is dispersed and homogenized under the conditions of controlling the temperature at 40-70°C and the rotation speed at 10,000-15,000 r / min. This allows the lutein to be fully and evenly dispersed in the third solution to form a uniform and stable oil phase system (i.e., the fourth solution).

[0047] Among them, ascorbyl palmitate has both emulsifying and antioxidant effects, which can promote the uniform dispersion of lutein in the oil phase system. At the same time, when used in combination with vitamin E, it has a synergistic antioxidant effect, which helps to delay the loss of part or all of the activity of lutein due to oxidation. In addition, the porous structure of silicon dioxide can provide sufficient adsorption sites, so that lutein can attach to these adsorption sites, which can effectively prevent the aggregation of lutein molecules due to mutual attraction, thereby further improving the uniform dispersion of lutein and forming a uniform and stable oil phase system.

[0048] In the above steps S3 to S6, by first splitting the second solution into the first aqueous phase and the second aqueous phase, and splitting the fourth solution into the first oil phase and the second oil phase, and then performing emulsification in steps, and controlling the order and speed of addition of the aqueous phase and the oil phase as well as the shear speed in each step, the oil phase and the aqueous phase in the emulsion system can be fully mixed during the emulsification process, thereby improving the stability of the emulsion and the uniformity of the particle size distribution, while also improving the preparation efficiency of the emulsion.

[0049] In some embodiments, in step S4, the first aqueous phase can be added to the first oil phase at a rate of 1% to 2% of the total mass of the first system per minute, and shear emulsification can be performed at a shear speed of 5,000 to 10,000 r / min to obtain a first emulsion. The total mass of the first system refers to the sum of the mass of the first aqueous phase and the mass of the first oil phase. For example, the shear speed can be 5,000 r / min, 8,000 r / min, or 10,000 r / min, and the shear time can be 3 to 5 minutes, for example, 3, 4, or 5 minutes.

[0050] In some embodiments, in step S5, the second aqueous phase can be added to the first emulsion at a rate of 3% to 5% of the total mass of the second system per minute, and shear emulsification is performed at a shear speed of 10,000 to 25,000 r / min to obtain the second emulsion. The total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion. For example, the shear speed can be 10,000 r / min, 12,000 r / min, 15,000 r / min, 20,000 r / min, or 25,000 r / min. The shear time can be 10 to 20 minutes, for example, 10, 15, or 20 minutes.

[0051] In some embodiments, in step S6, the second oil phase can be added to the second emulsion at a rate of 1% to 3% of the total mass of the third system per minute, and shear emulsification can be performed at a shear speed of 10,000 to 25,000 r / min to obtain a third emulsion. The total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion. For example, the shear speed can be 10,000 r / min, 12,000 r / min, 15,000 r / min, 20,000 r / min, or 25,000 r / min. The shear time can be 10 to 20 minutes, for example, 10, 15, or 20 minutes.

[0052] In some embodiments, in step S7, the third emulsion obtained in step S6 is subjected to high-pressure homogenization at a pressure of 30 to 120 MPa, for example, 30 MPa, 40 MPa, 50 MPa, 60 MPa, 70 MPa, 80 MPa, 90 MPa, 100 MPa, 110 MPa, or 120 MPa. Thereafter, spray drying is performed, with the inlet air temperature of the spray drying process being controlled at 150 to 170°C, for example, 150°C, 155°C, 160°C, 165°C, or 170°C. The outlet air temperature of the spray drying process is controlled at 30 to 80°C, for example, 30°C, 40°C, 50°C, 60°C, 70°C, or 80°C.

[0053] The lutein microcapsule powder prepared in the above embodiment is an O / W type microcapsule powder, which has cold water dispersibility, good rehydration stability, high embedding rate, good sustained-release performance, and high bioavailability.

[0054] The composite dietary composition for improving microcirculation provided in the embodiments of the present application is compounded with freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, sophora japonica rice powder, (3R,3'R)-dihydroxy-β-carotene, and is added with matcha powder, multivitamins, fermented ginger powder and lutein microcapsule powder. It contains rich nutrients. When consumed in moderation, it can supplement the body with the required nutrients and maintain the body's nutritional balance. At the same time, it has a good effect of adjusting microcirculation, promoting capillary softening, and promoting ocular microcirculation.

[0055] In some embodiments, the lutein microcapsule powder includes the following components in parts by weight: 10-20 parts lutein, 1-100 parts maltooligosaccharide, 10-50 parts octenylsuccinate monoarabic gum, 5-7 parts vitamin E, 5-10 parts silicon dioxide, and 1-2 parts ascorbyl palmitate. The lutein microcapsule powder prepared using the preparation method of the present invention has a diameter of 1-100 nm and a wall thickness of 0.5-150 nm.

[0056] In some embodiments, the preparation steps of the fermented ginger powder are as follows:

[0057] Step 1: Wash, crush, squeeze and filter the ginger to obtain ginger juice;

[0058] Step 2: Sterilize the ginger juice, inoculate the fermentation bacteria, and ferment at 25-35° C. for at least 48 hours to obtain a ginger fermentation product;

[0059] Step 3: Drying the fermented ginger to obtain fermented ginger powder.

[0060] In some embodiments, in the above step 1, the ginger can be washed, peeled, and sliced ​​to obtain ginger slices, and then the ginger slices can be crushed, squeezed, and filtered to obtain ginger juice.

[0061] In some embodiments, in the above step 2, the ginger juice obtained in the above step 1 is placed in a stainless steel container, sealed, placed in an oven, and pasteurized at a temperature of 80-85°C. Thereafter, the container is taken out and cooled to room temperature, inoculated with fermentation bacteria, and fermented at 25-35°C for at least 48 hours to obtain a ginger fermentation product.

[0062] The fermentation strain may be one or a combination of Candida utilis, Acetobacter, or Aspergillus oryzae. For example, the fermentation strain may be any one of Candida utilis, Acetobacter, or Aspergillus oryzae; or a combination of Candida utilis and Aspergillus oryzae; or a combination of Candida utilis and Acetobacter; or a combination of Acetobacter and Aspergillus oryzae; or a combination of Candida utilis, Acetobacter, and Aspergillus oryzae.

[0063] Candida utilis can utilize sugars, nitrogen sources and other substances in ginger for growth and fermentation, producing rich nutrients such as protein and vitamins, which can improve the nutritional value of ginger fermented products. At the same time, it can also produce some flavor substances to improve the flavor of the product.

[0064] Acetobacter can produce some enzymes that can break down macromolecules in ginger, such as proteins and polysaccharides, making the nutrients in ginger easier for the human body to absorb and utilize. At the same time, fermentation may also produce some new bioactive substances, such as certain antioxidants and probiotics, thereby improving the nutritional value and health benefits of ginger products.

[0065] Aspergillus oryzae can produce a variety of enzymes, such as protease, amylase, saccharifying enzyme, etc., which can break down macromolecules such as protein and starch in ginger into small molecular peptides, amino acids, glucose, etc., which is beneficial to improving the fermentation efficiency of ginger and the quality of the product, and can be used in the production of ginger fermented seasonings and other products.

[0066] Experimental studies have found that when a combination of Candida utilis, Acetobacter and Aspergillus oryzae is used as a fermentation strain to ferment ginger juice, and the ratio of the number of live bacteria of Candida utilis, Acetobacter and Aspergillus oryzae is 10:2~5:1~2, the spiciness and slight bitterness of ginger can be effectively reduced, thereby improving the taste. In addition, using a combination of the above three strains to ferment ginger juice can not only improve the fermentation effect, but also better maintain the antioxidant activity of components such as 6-gingerol and gingerol in ginger.

[0067] Optionally, the mass ratio of the fermentation bacteria to the ginger juice is 1:100-150. For example, the mass ratio of the fermentation bacteria to the ginger juice can be 1:100, 1:110, 1:120, 1:130, 1:140 or 1:150, etc.

[0068] In some embodiments, the preparation steps of the above-mentioned sophora japonica rice powder are as follows:

[0069] Step 1, grinding the dried Sophora japonica seeds and then performing supercritical carbon dioxide inactivation treatment to obtain an enzyme-inactivated product;

[0070] Step 2: adding the enzyme-killed substance to an extraction solvent for extraction and separation to obtain an organic phase containing the effective ingredients of Sophora japonica seeds; wherein the extraction solvent comprises the following components in parts by weight: 10-40 parts of polyvinyl alcohol, 5-40 parts of an acidic inorganic salt, 70-90 parts of water, and 2-10 parts of chitosan;

[0071] Step 3: Concentrate the organic phase under reduced pressure to precipitate crystals to obtain Sophora japonica flour.

[0072] In some embodiments, in the above step 1, the material-liquid ratio of the enzyme-inactivated Sophora japonica flower to the extraction solvent is 1:6-10; for example, it can be 1:6, 1:7, 1:8, 1:9 or 1:10, etc.

[0073] In some embodiments, in the above step 1, the extraction conditions are: extraction time is 10-15 minutes (for example, it can be 10, 12 or 15 minutes, etc.), extraction temperature is 25-70°C (for example, it can be 25°C, 30°C, 40°C, 50°C, 60°C or 70°C, etc.), and pH value is 4-6 (for example, it can be 4, 4.5, 5, 5.5 or 6, etc.).

[0074] In some embodiments, in the above step 2, the acidic inorganic salt may be ammonium sulfate or the like.

[0075] A three-phase aqueous system is formed by compounding polyvinyl alcohol, acidic inorganic salt, chitosan and water, wherein polyvinyl alcohol can form a two-phase aqueous system with acidic inorganic salt and chitosan respectively, and acidic inorganic salt and chitosan can also form a two-phase aqueous system.

[0076] Polyvinyl alcohol molecules contain a large number of hydroxyl groups, and chitosan molecules contain hydroxyl and amino groups. The two can interact with each other through hydrogen bonds. Under certain conditions, this hydrogen bonding will cause the polymer molecules to aggregate to form different phase regions, thereby leading to phase separation. In addition, chitosan will be protonated in acidic solutions and carry a positive charge, and some hydroxyl groups in polyvinyl alcohol molecules may be ionized and carry a negative charge. The mutual attraction between positive and negative charges also plays a certain role in phase separation.

[0077] In acidic solution, the amino groups on the chitosan molecular chain will be protonated and carry a positive charge; acidic inorganic salts will ionize into anions and cations in the solution. Anions can interact with protonated chitosan through electrostatic attraction, while cations will form a certain ionic atmosphere in the solution, affecting the charge balance and intermolecular forces of the system. When a certain concentration and conditions are reached, it may cause the system to phase separate and form a two-phase aqueous solution.

[0078] By adding the enzyme-inactivating substance to a three-phase system composed of polyvinyl alcohol, acidic inorganic salts, chitosan and water, the effective ingredients of Sophora japonica (such as rutin, etc.) can be selectively transferred to the polyvinyl alcohol phase and / or chitosan phase enrichment phase in the three-phase system, while the impurities remain in the acidic inorganic salt phase, resulting in higher extraction efficiency and better purification effect.

[0079] Rutin belongs to vitamin P, which can reduce the permeability and fragility of capillaries, and can maintain and restore the normal elasticity of capillaries.

[0080] In some embodiments, the complex dietary composition includes the following components in parts by weight: 30-40 parts of freeze-dried fruit and vegetable composite powder, 1-3 parts of European blueberry concentrated powder, 4-7 parts of sophora japonica rice powder, 0.1-5 parts of (3R,3'R)-dihydroxy-β-carotene, 0.1-0.5 parts of lutein microcapsule powder, 30-40 parts of matcha powder, 0.01-2 parts of fermented ginger powder, and 2-7 parts of complex vitamins.

[0081] The freeze-dried fruit and vegetable composite powder includes at least one of kale powder, hawthorn powder, pomegranate powder, blackcurrant powder, acerola cherry powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder or bitter melon powder.

[0082] Optionally, the freeze-dried fruit and vegetable compound powder includes kale powder, hawthorn powder, pomegranate powder, black currant powder, acerola cherry powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder and bitter melon powder.

[0083] Among them, kale powder is rich in anthocyanins and dietary fiber. Hawthorn powder is rich in brass and organic acids. Pomegranate powder is rich in ellagic acid and organic acids. Blackcurrant powder is rich in flavonoids, vitamin C, and anthocyanins. Acerola cherry powder is rich in vitamin C and B vitamins. Sea buckthorn fruit powder is rich in vitamin C, flavonoids, and polyphenols. Watermelon powder is rich in lycopene. Carrot powder is rich in beta-carotene. Tomato powder is rich in lycopene. Spinach powder is rich in nitrates and potassium. Barley grass powder is rich in protein and dietary fiber. Celery powder is rich in apigenin and nitrates. Bitter melon powder is rich in bitter melon peptides.

[0084] In some embodiments, the freeze-dried fruit and vegetable composite powder can be prepared using a vacuum freeze-drying process (temperature of -30°C), which can retain the nutrients such as vitamins, minerals, proteins and polyphenols in the raw materials to the greatest extent; and the color is close to the original color of fruits and vegetables.

[0085] The vacuum freeze-drying process can also be called the freeze-drying process. Under high vacuum conditions, the sublimation principle is used to remove the moisture in the pre-frozen material by directly sublimating the moisture into water vapor in a solid state without melting the ice.

[0086] The advantages of using vacuum freeze drying to prepare freeze-dried fruit and vegetable composite powders include: (1) Since the water is directly sublimated in a solid state, the color, texture, and shape of the product remain essentially unchanged, while the nutrients such as vitamins, minerals, proteins, and polyphenols in the food raw materials can be retained to the greatest extent. (2) Since the drying process is carried out at low temperature and under vacuum conditions, the activity of some heat-sensitive compounds and easily oxidized ingredients will not be affected.

[0087] In some embodiments, the vitamin complex includes at least three of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D, and vitamin E.

[0088] Optionally, the multivitamin includes vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D and vitamin E.

[0089] In some embodiments, the freeze-dried fruit and vegetable composite powder is selected from kale powder, hawthorn powder, pomegranate powder, blackcurrant powder, acerola cherry powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder, and bitter melon powder; and the composite vitamins are selected from vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D, and vitamin E. The combination of 13 fruit and vegetable essences and 12 vitamins can promote energy metabolism in the body.

[0090] In some embodiments, the complex dietary composition for improving microcirculation includes 30-40 parts of freeze-dried fruit and vegetable composite powder, 1-3 parts of European blueberry concentrated powder, 4-7 parts of sophora japonica rice powder, 0.1-5 parts of (3R, 3'R)-dihydroxy-β-carotene, 0.1-0.5 parts of lutein microcapsule powder, 30-40 parts of matcha powder, 0.01-2 parts of fermented ginger powder and 2-7 parts of complex vitamins, 0.1-0.5 parts of sorbitol, 0.2-0.5 parts of microcrystalline cellulose, 0.5-1 parts of β-carotene and 0.01-0.5 parts of magnesium stearate. Among them, freeze-dried fruit and vegetable compound powder includes kale powder, hawthorn powder, pomegranate powder, black currant powder, acerola cherry powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder and bitter melon powder; complex vitamins include vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D and vitamin E.

[0091] Among them, European blueberry concentrate powder is rich in anthocyanins, which can stabilize the microvessels in the eyes and enhance microcirculation. Anthocyanins are also strong antioxidants that can reduce the damage of free radicals to the eyes and help prevent cataracts.

[0092] Lutein and (3R,3'R)-dihydroxy-β-carotene co-exist in the retina of the eye, which can help the eyes block harmful blue light and protect the macula of the retina from damage. According to relevant studies, increasing the intake of (3R,3'R)-dihydroxy-β-carotene and lutein can reduce the risk of cataracts.

[0093] Vitamin A is an important raw material for the photosensitive substances in the eyes. Vitamin A deficiency can cause the corneal epithelial cells to fall off, thicken, and keratinize, making the originally clear and transparent cornea become as blurry as frosted glass, and even cause night blindness, cataracts and other eye diseases.

[0094] Vitamin E has strong antioxidant properties, which can reduce free radicals in the eyeball and delay eye aging.

[0095] Vitamin C has antioxidant effects, can prevent light-induced oxidation reactions, prevent retinal damage from ultraviolet rays, prevent lens aging, and increase the toughness of eye microvessels; it can help prevent cataracts, prevent lens clouding, and improve symptoms of blurred vision and double vision.

[0096] The composite dietary composition for improving microcirculation in the embodiment of the present application is specially supplemented with nutrients required for ocular microcirculation: European blueberry concentrate powder, lutein, (3R,3'R)-dihydroxy-β-carotene, β-carotene, vitamin A, vitamin E and vitamin C. These nutrients coordinate with each other and work together to adjust microcirculation, promote capillary softening, and promote ocular microcirculation.

[0097] The composite dietary composition for improving microcirculation in the embodiment of the present application can assist in dilating blood vessels and promoting blood flow by adding fermented ginger powder and freeze-dried fruit and vegetable composite powder, and the fermented ginger powder also has the effect of inhibiting platelet aggregation and preventing thrombosis; by adding sophora japonica powder, fermented ginger powder and vitamin D, it can assist in repairing the vascular endothelium; by adding sophora japonica powder, vitamin E and vitamin C, it can assist in repairing the elasticity of capillaries; by adding European blueberry concentrate powder, vitamin C, vitamin E, matcha powder, lutein microcapsule powder, (3R,3'R)-dihydroxy-β-carotene and β-carotene, it can play an antioxidant and protect microvessel role; by adding B vitamins, it can promote cell metabolism.

[0098] In a second aspect, the present invention further provides a method for preparing the composite dietary composition for improving microcirculation according to the first aspect, comprising:

[0099] Freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, sophora japonica rice powder, (3R, 3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and complex vitamins are mixed with a tablet flow aid, and tablets are formed to obtain the product.

[0100] In some embodiments, the tablet glidant includes magnesium stearate. Magnesium stearate has good fluidity and compressibility and is used as a glidant in direct tableting to facilitate tablet formation.

[0101] Optionally, the mass ratio of the tablet glidant to the total amount of raw materials of the complex dietary composition for improving microcirculation is 0.1% to 0.5%, for example, it can be 0.1%, 0.2%, 0.3%, 0.4% or 0.5%.

[0102] The present application has been subjected to multiple tests, and part of the test results are cited as a reference to further describe the invention in detail, which will be described in detail in conjunction with specific embodiments.

[0103] Example 1

[0104] The composite dietary composition for improving microcirculation of this embodiment includes the raw material components shown in Table 1:

[0105] Table 1

[0106]

[0107] Among them, the above-mentioned freeze-dried fruit and vegetable composite powder includes 28 g of kale powder, 30 g of hawthorn powder, 32 g of pomegranate powder, 15 g of blackcurrant powder, 23 g of acerola powder, 35 g of sea buckthorn fruit powder, 28 g of watermelon powder, 36 g of carrot powder, 42 g of tomato powder, 37 g of spinach powder, 20 g of barley leaf powder, 20.5 g of celery powder and 20 g of bitter melon powder.

[0108] The above-mentioned European blueberry concentrated powder is European blueberry concentrated powder made from wild European blueberry fruits imported from Sweden.

[0109] The above-mentioned complex vitamins include vitamin B1 2g, vitamin B2 4g, vitamin B3 1g, vitamin B52.5g, vitamin B6 4.5g, vitamin B7 8g, vitamin B12 2.5g, vitamin C 6g, vitamin A 8g, vitamin D6.5g and vitamin E 5g.

[0110] The above-mentioned sophora japonica rice flour is prepared by the following steps:

[0111] The dried Sophora japonica seeds are crushed and then subjected to supercritical carbon dioxide enzyme inactivation treatment to obtain an enzyme-inactivated product; the enzyme-inactivated product is added to an extraction solvent for extraction and separation to obtain an organic phase containing the effective components of the Sophora japonica seeds; wherein the extraction solvent comprises the following components in parts by weight: 10 parts polyvinyl alcohol, 5 parts ammonium sulfate (acidic inorganic salt), 70 parts water, and 3 parts chitosan; the solid-liquid ratio of the enzyme-inactivated Sophora japonica seeds to the extraction solvent is 1:6; the extraction time is 10 minutes, the extraction temperature is 35°C, and the pH value is 4;

[0112] The organic phase is concentrated under reduced pressure to precipitate crystals to obtain sophora japonica flour.

[0113] The above-mentioned lutein microcapsule powder is prepared by the following steps:

[0114] S1. Prepare the following ingredients: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinate monoarabic gum 100 mg, vitamin E 50 mg, silicon dioxide 60 mg, and ascorbyl palmitate 10 mg.

[0115] S2. Dissolving a composite emulsifier in water to prepare a first solution, then adding octenylsuccinate monoarabic gum ester to the first solution and mixing to obtain a second solution; the composite emulsifier is a composition comprising mealworm protein, maltooligosaccharide, and Tween in a mass ratio of 0.1:1:0.5. The mass ratio of octenylsuccinate monoarabic gum ester to the composite emulsifier is 1:0.5.

[0116] S3. Add ascorbyl palmitate to oil and heat to dissolve to obtain a third solution. Add lutein, vitamin E, and silicon dioxide to the third solution, and disperse and homogenize at a temperature of 70° C. and a rotation speed of 10,000 r / min to obtain a fourth solution.

[0117] S4. Splitting the second solution into a first aqueous phase and a second aqueous phase, wherein the weight ratio of the first aqueous phase to the second aqueous phase is 2:8; splitting the fourth solution into a first oil phase and a second oil phase, wherein the weight ratio of the first oil phase to the second oil phase is 2:8.

[0118] S5. Add the first aqueous phase to the first oil phase at a rate of 1% of the total mass of the first system per minute, control the temperature to 25°C and the pH to 4, and perform shear emulsification at a shear speed of 5000 r / min to obtain a first emulsion, wherein the total mass of the first system refers to the sum of the mass of the first aqueous phase and the mass of the first oil phase.

[0119] S6. Add the second aqueous phase to the first emulsion at a rate of 3% of the total mass of the second system per minute, control the temperature to 25°C and the pH to 4, and perform shear emulsification at a shear speed of 10,000 r / min to obtain a second emulsion, wherein the total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion.

[0120] S7. Add the second oil phase to the second emulsion at a rate of 2% of the total mass of the third system per minute, control the temperature to 25°C and the pH to 4, and perform shear emulsification at a shear speed of 25,000 r / min to obtain a third emulsion, wherein the total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion.

[0121] S8. High-pressure homogenization and spray drying of the third emulsion to obtain lutein microcapsule powder, wherein the homogenization pressure is 120 MPa.

[0122] The fermented ginger powder is prepared by the following steps:

[0123] Step 1: Wash, crush, squeeze and filter the ginger to obtain ginger juice;

[0124] Step 2: The ginger juice is placed in a stainless steel container, sealed, and placed in an oven for pasteurization at 80°C. The container is then taken out, cooled to room temperature, inoculated with Aspergillus oryzae, and fermented at 30°C for at least 48 hours to obtain a ginger fermentation product.

[0125] Step 3: Drying the fermented ginger to obtain fermented ginger powder.

[0126] The steps for preparing the composite dietary composition for improving microcirculation in the embodiment of the present application are as follows:

[0127] Weigh the raw materials according to the formula shown in Table 1 and set aside;

[0128] 366.5 g of freeze-dried fruit and vegetable composite powder, 12 g of European blueberry concentrated powder, 43.5 g of sophora japonica rice powder, 1.25 g of (3R,3'R)-dihydroxy-β-carotene, 3.25 g of lutein microcapsule powder, 312.5 g of matcha powder, 10.5 g of fermented ginger powder, and 50 g of multivitamins were mixed with 2.4 g of magnesium stearate and compressed into tablets to obtain tablets of a composite dietary composition for improving microcirculation.

[0129] Example 2

[0130] The composite dietary composition for improving microcirculation of this embodiment includes the raw material components shown in Table 2:

[0131] Table 2

[0132]

[0133] The freeze-dried fruit and vegetable composite powder includes 20 g of kale powder, 25 g of hawthorn powder, 22 g of pomegranate powder, 15 g of blackcurrant powder, 20 g of acerola powder, 25 g of sea buckthorn fruit powder, 25 g of watermelon powder, 30 g of carrot powder, 22 g of tomato powder, 35.5 g of spinach powder, 20 g of barley leaf powder, 20.5 g of celery powder and 20 g of bitter melon powder.

[0134] The above-mentioned European blueberry concentrated powder is European blueberry concentrated powder made from wild European blueberry fruits imported from Sweden.

[0135] The above-mentioned complex vitamin includes vitamin B1 5g, vitamin B2 5g, vitamin B3 1g, vitamin B5 3g, vitamin B6 5g, vitamin B7 8g, vitamin B12 5g, vitamin C 8g, vitamin A 10g, vitamin D 10g and vitamin E 9g.

[0136] The preparation method of the sophora japonica flour of the present embodiment is the same as the preparation method of the sophora japonica flour of above-mentioned embodiment 1.

[0137] The above-mentioned lutein microcapsule powder is prepared by the following steps:

[0138] S1. Prepare the following ingredients: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinate monoarabic gum 100 mg, vitamin E 50 mg, silicon dioxide 60 mg, and ascorbyl palmitate 10 mg.

[0139] S2. Dissolving a composite emulsifier in water to prepare a first solution, then adding octenylsuccinate monoarabic gum ester to the first solution and mixing to obtain a second solution; the composite emulsifier is a composition comprising mealworm protein, maltooligosaccharide, and Tween in a mass ratio of 0.1:1:0.5. The mass ratio of octenylsuccinate monoarabic gum ester to the composite emulsifier is 1:0.1.

[0140] S3. Add ascorbyl palmitate to oil and heat to dissolve to obtain a third solution. Add lutein, vitamin E, and silicon dioxide to the third solution and disperse and homogenize at a temperature of 60° C. and a rotation speed of 15,000 r / min to obtain a fourth solution.

[0141] S4. Splitting the second solution into a first aqueous phase and a second aqueous phase, wherein the weight ratio of the first aqueous phase to the second aqueous phase is 2:8; splitting the fourth solution into a first oil phase and a second oil phase, wherein the weight ratio of the first oil phase to the second oil phase is 2:8.

[0142] S5. Add the first aqueous phase to the first oil phase at a rate of 2% of the total mass of the first system per minute, control the temperature to 35°C and the pH to 5, and perform shear emulsification at a shear speed of 6000 r / min to obtain a first emulsion, wherein the total mass of the first system refers to the sum of the mass of the first aqueous phase and the mass of the first oil phase.

[0143] S6. Add the second aqueous phase to the first emulsion at a rate of 5% of the total mass of the second system per minute, control the temperature to 35°C and the pH to 5, and perform shear emulsification at a shear speed of 20,000 r / min to obtain a second emulsion, wherein the total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion.

[0144] S7. Add the second oil phase to the second emulsion at a rate of 3% of the total mass of the third system per minute, control the temperature to 35°C and the pH to 5, and perform shear emulsification at a shear speed of 25,000 r / min to obtain a third emulsion, wherein the total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion.

[0145] S8. High-pressure homogenization and spray drying of the emulsion to obtain lutein microcapsule powder, wherein the homogenization pressure is 80 MPa.

[0146] The preparation method of the fermented ginger powder of this embodiment is the same as the preparation method of the fermented ginger powder of the above-mentioned embodiment 1.

[0147] The steps for preparing the composite dietary composition for improving microcirculation in the embodiment of the present application are as follows:

[0148] Weigh the raw materials according to the formula shown in Table 2 and set aside;

[0149] 300 g of freeze-dried fruit and vegetable composite powder, 120 g of European blueberry concentrated powder, 50 g of sophora japonica rice powder, 1.25 g of (3R,3'R)-dihydroxy-β-carotene, 3.5 g of lutein microcapsule powder, 312.5 g of matcha powder, 11 g of fermented ginger powder, and 69 g of multivitamins were mixed with 2.0 g of magnesium stearate and compressed into tablets to obtain tablets of a composite dietary composition for improving microcirculation.

[0150] Example 3

[0151] The composite dietary composition for improving microcirculation of this embodiment includes the raw material components shown in Table 3:

[0152] Table 3

[0153]

[0154] Among them, the above-mentioned freeze-dried fruit and vegetable composite powder includes 38 g of kale powder, 30 g of hawthorn powder, 22 g of pomegranate powder, 25 g of blackcurrant powder, 30 g of acerola powder, 35 g of sea buckthorn fruit powder, 28 g of watermelon powder, 35 g of carrot powder, 35 g of tomato powder, 42 g of spinach powder, 30 g of barley leaf powder, 26 g of celery powder and 24 g of bitter melon powder.

[0155] The above-mentioned complex vitamins include vitamin B1 2.5g, vitamin B2 1.8g, vitamin B3 1.5g, vitamin B5 1.65g, vitamin B6 1.45g, vitamin B7 2.8g, vitamin B12 1.6g, vitamin C 1.65g, vitamin A2.5g, vitamin D 4g and vitamin E 3.55g.

[0156] The above-mentioned lutein microcapsule powder is prepared by the following steps:

[0157] S1. Prepare the following ingredients: lutein 200 mg, maltooligosaccharide 1000 mg, octenylsuccinate monoarabic gum 500 mg, vitamin E 70 mg, silicon dioxide 100 mg, and ascorbyl palmitate 20 mg.

[0158] S2. Dissolving a composite emulsifier in water to prepare a first solution, then adding octenylsuccinate monoarabic gum ester to the first solution and mixing to obtain a second solution; the composite emulsifier is a composition comprising mealworm protein, maltooligosaccharide, and Tween in a mass ratio of 0.1:1:0.5. The mass ratio of octenylsuccinate monoarabic gum ester to the composite emulsifier is 1:0.3.

[0159] S3. Add ascorbyl palmitate to oil and heat to dissolve to obtain a third solution. Add lutein, vitamin E, and silicon dioxide to the third solution and disperse and homogenize at a temperature of 70° C. and a rotation speed of 10,000 r / min to obtain a fourth solution.

[0160] S4. Splitting the second solution into a first aqueous phase and a second aqueous phase, wherein the weight ratio of the first aqueous phase to the second aqueous phase is 2:8; splitting the fourth solution into a first oil phase and a second oil phase, wherein the weight ratio of the first oil phase to the second oil phase is 2:8.

[0161] S5. Add the first aqueous phase to the first oil phase at a rate of 2% of the total mass of the first system per minute, control the temperature to 45°C and the pH to 7, and perform shear emulsification at a shear speed of 10,000 r / min to obtain a first emulsion, wherein the total mass of the first system refers to the sum of the mass of the first aqueous phase and the mass of the first oil phase.

[0162] S6. Add the second aqueous phase to the first emulsion at a rate of 4% of the total mass of the second system per minute, control the temperature to 45°C and the pH to 7, and perform shear emulsification at a shear speed of 20,000 r / min to obtain a second emulsion, wherein the total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion.

[0163] S7. Add the second oil phase to the second emulsion at a rate of 3% of the total mass of the third system per minute, control the temperature to 45°C and the pH to 7, and perform shear emulsification at a shear speed of 25,000 r / min to obtain a third emulsion, wherein the total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion.

[0164] S8. High-pressure homogenization and spray drying of the emulsion to obtain lutein microcapsule powder, wherein the homogenization pressure is 120 MPa.

[0165] The raw materials of this embodiment, the European blueberry concentrated powder, the sophora japonica rice powder, and the fermented ginger powder, are the same as the raw materials of Example 1, the European blueberry concentrated powder, the sophora japonica rice powder, and the fermented ginger powder.

[0166] The steps for preparing the composite dietary composition for improving microcirculation in the embodiment of the present application are as follows:

[0167] Weigh the raw materials according to the formula shown in Table 3 and set aside;

[0168] 400 g of freeze-dried fruit and vegetable composite powder, 200 g of European blueberry concentrated powder, 70 g of sophora japonica rice powder, 5 g of (3R,3'R)-dihydroxy-β-carotene, 4 g of lutein microcapsule powder, 200 g of matcha powder, 15 g of fermented ginger powder, and 25 g of multivitamins were mixed with 2.4 g of magnesium stearate and tableted to obtain a composite dietary composition tablet for improving microcirculation with a tablet weight of 0.8 g.

[0169] Comparative Example 1

[0170] The microcirculation-improving composite dietary composition of this comparative example includes the raw material components shown in Table 4:

[0171] Table 4

[0172]

[0173] The raw materials in Table 4 were used to prepare the composite dietary composition tablets for improving microcirculation of this comparative example by referring to the preparation method of Example 1.

[0174] Comparative Example 2

[0175] The composite dietary composition for improving microcirculation of this comparative example includes the raw material components shown in Table 5:

[0176] Table 5

[0177]

[0178] The raw materials in Table 5 were used, and the preparation method of Example 1 was referred to to prepare the composite dietary composition tablets for improving microcirculation of this comparative example.

[0179] Comparative Example 3

[0180] The composite dietary composition for improving microcirculation of this comparative example includes the raw material components shown in Table 6:

[0181] Table 6

[0182]

[0183] The raw materials in Table 6 were used to prepare the composite dietary composition tablets for improving microcirculation of this comparative example by referring to the preparation method of Example 1.

[0184] Comparative Example 4

[0185] The composite dietary composition for improving microcirculation of this comparative example includes the raw material components shown in Table 7:

[0186] Table 7

[0187]

[0188] The raw materials in Table 7 were used to prepare the composite dietary composition tablets for improving microcirculation of this comparative example by referring to the preparation method of Example 1.

[0189] Comparative Example 5

[0190] The composite dietary composition for improving microcirculation of this comparative example includes the raw material components shown in Table 8:

[0191] Table 8

[0192]

[0193] The raw materials in Table 8 were used, and the preparation method of Example 1 was referred to to prepare the composite dietary composition tablets for improving microcirculation of this comparative example.

[0194] Wherein, the preparation steps of common ginger powder are as follows:

[0195] Step 1: Wash, crush, squeeze and filter the ginger to obtain ginger juice;

[0196] Step 2: Dry the ginger juice obtained in step 1 to obtain ordinary ginger powder.

[0197] Comparative Example 6

[0198] The composite dietary composition of this comparative example differs from the composite dietary composition of Example 1 only in that the lutein microcapsule powder is composed of the following components in parts by weight: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinate monoarabic gum ester 100 mg, vitamin E 50 mg, and ascorbyl palmitate 10 mg.

[0199] Comparative Example 7

[0200] The composite dietary composition of this comparative example differs from the composite dietary composition of Example 1 only in that the lutein microcapsule powder is composed of the following components in parts by weight: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinate monoarabic gum ester 100 mg, silicon dioxide 60 mg, and ascorbic acid palmitate 10 mg.

[0201] Comparative Example 8

[0202] The composite dietary composition of this comparative example differs from the composite dietary composition of Example 1 only in that the lutein microcapsule powder is composed of the following components in parts by weight: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinate monoarabic gum ester 100 mg, vitamin E 50 mg, and silicon dioxide 60 mg.

[0203] Comparative Example 9

[0204] The composite dietary composition of this comparative example differs from the composite dietary composition of Example 1 only in that: lutein ester 100 mg, maltooligosaccharide 500 mg, octenylsuccinate monoarabic gum ester 100 mg, vitamin E 50 mg, silicon dioxide 60 mg, and ascorbic acid palmitate 10 mg.

[0205] In order to further illustrate the improvement effect of the composite dietary composition for improving microcirculation prepared in the examples of the present application on ocular microcirculation, the following performance tests were conducted on the tablets of the composite dietary composition for improving microcirculation prepared in Examples 1 to 3 and Comparative Examples 1 to 9 of the present application.

[0206] 1) Test object:

[0207] A total of 80 subjects aged 20 to 45 years with symptoms of conjunctival arteriovenous constriction, cystic dilatation and spiral curvature of the conjunctiva, and frequent microvascular exudation, bleeding, and hemosiderin deposition were selected. The male-to-female ratio was approximately 1:1. They were randomly divided into 3 groups (Group 1 to Group 12), with 10 subjects in each group. The male-to-female ratio of each group was approximately 1:1.

[0208] 2) Testing instruments and methods:

[0209] Conjunctival microcirculation and microvasculature were assessed using an integrated video and recording microcirculatory instrument (developed by the Shanghai Laser Technology Research Institute). During the test, the subject sat with their head resting on a microcirculatory support. Changes in the temporal conjunctival microcirculation and microvasculature were observed. Scoring and analysis were performed using Tian's comprehensive quantitative microcirculatory method.

[0210] During fundus angiography, the pupil is first dilated, and then 3 ml (0.6 g) of sodium fluorescein is injected into the subject's forearm vein. The fundus blood vessels are immediately observed using a TOPCON slit lamp microscope and continuous photographs are taken, and the results are analyzed.

[0211] 3) Usage and dosage:

[0212] The 10 subjects in Group 1 took the microcirculation-improving composite dietary composition tablets prepared in Example 1 of the present application, 2 tablets each time, twice a day (once in the morning and once in the evening), which can be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0213] The 10 subjects in Group 2 took the microcirculation-improving composite dietary composition tablets prepared in Example 2 of the present application, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0214] The 10 subjects in Group 3 took the microcirculation-improving composite dietary composition tablets prepared in Example 3 of the present application, 2 tablets each time, twice a day (once in the morning and once in the evening), which can be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0215] The 10 subjects in Group 4 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 1, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0216] The 10 subjects in Group 5 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 2, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0217] The 10 subjects in Group 6 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 3, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0218] The 10 subjects in Group 7 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 4, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0219] The 10 subjects in Group 8 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 5, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0220] The 10 subjects in Group 9 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 6, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0221] The 10 subjects in Group 10 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 7, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0222] The 10 subjects in Group 11 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 8, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0223] The 10 subjects in Group 12 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 9, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0224] 4) Statistical analysis:

[0225] After three courses of treatment, the results of the conjunctival microcirculation test of the subjects in Groups 1 to 12 were comprehensively and quantitatively analyzed. The results are shown in Table 9 below.

[0226] Table 9 Changes in the integral value of bulbar conjunctival microcirculation of the three groups of subjects before and after treatment (x±s)

[0227]

[0228]

[0229] Note: In Table 9, * indicates p < 0.01 compared with before treatment, and # indicates p < 0.05 compared with before treatment.

[0230] As can be seen from Table 9 above, after three courses of treatment with the composite dietary composition tablets for improving microcirculation provided by Examples 1 to 3 of the present application, the subjects in Groups 1 to 3 respectively had varying degrees of reduction in the total score of the bulbar conjunctiva, and the bulbar conjunctival microcirculation was significantly improved. This indicates that the composite dietary composition tablets for improving microcirculation provided by the examples of the present application have a good effect of regulating microcirculation, promoting capillary softening, and promoting ocular microcirculation.

[0231] After three courses of treatment with the microcirculation-improving composite dietary composition tablets provided in Comparative Examples 1-5, the subjects in Groups 4-8 all showed varying degrees of reduction in their total conjunctival score and significant improvement in conjunctival microcirculation, although the improvement was slightly less pronounced than in Groups 1-3. This indicates that the simultaneous addition of (3R,3'R)-dihydroxy-β-carotene, lutein microcapsule powder, sophora japonica rice powder, and fermented ginger powder to the product exhibits a synergistic effect, thereby achieving enhanced microcirculation regulation, capillary softening, and ocular microcirculation promotion.

[0232] After three courses of treatment with the microcirculation-improving composite dietary composition tablets provided in Comparative Examples 6 to 8, the subjects in Groups 9 to 11 all showed varying degrees of reduction in their total conjunctival score and significant improvement in conjunctival microcirculation, although the improvement was slightly less than that seen in Groups 1 to 3. This indicates that the simultaneous addition of ascorbyl palmitate, vitamin E, and silicon dioxide to the preparation of lutein microcapsule powder has a synergistic effect, effectively delaying the partial or complete loss of lutein activity due to oxidation, improving the uniform dispersion of lutein, and enhancing its bioavailability, thereby achieving better microcirculation regulation, capillary softening, and ocular microcirculation promotion.

[0233] After three courses of treatment with the microcirculation-improving composite dietary composition tablets provided in Comparative Example 9, the subjects in Group 12 showed no significant difference in the total conjunctival score before and after treatment, nor was there a significant improvement in conjunctival microcirculation. This indicates that, compared to the lutein ester microcapsule powder, the lutein microcapsule powder used in the present example achieves better microcirculation regulation, capillary softening, and ocular microcirculation.

[0234] In addition, the present application has found through a large number of experiments that the stability and sustained-release properties of the lutein microcapsule powder provided in the examples of the present application will be significantly affected by changes in the preparation process conditions of the lutein microcapsule powder, as shown in the following experiments.

[0235] Experiment 1: Exploring the effects of different wall materials on the quality of lutein microencapsulated powder products

[0236] Each group of experiments used the same experimental conditions as the lutein microcapsule powder of Example 1 above, except that the wall material (octenylsuccinate monoarabic gum ester: composite emulsifier = 1:0.5 (mass ratio)) in Example 1 was replaced with the various materials listed in Tables 10 to 12 below of equal mass. The following performance index tests were performed on the prepared lutein microcapsule powders of each group. The test results are shown in Tables 10 to 12:

[0237] Among them, the test method / evaluation method of each performance indicator is as follows:

[0238] 1) Surface oil content:

[0239] The method specified in Appendix A of SC / T 3505-2006 “Fish Oil Microcapsules” was followed.

[0240] 2) In vitro dissolution:

[0241] In vitro dissolution testing was conducted according to the dissolution and release rate determination method specified in General Chapter 0931 of the 2020 edition of the Chinese Pharmacopoeia (Volume IV). Lutein solubility was measured in artificial intestinal fluid containing trypsin (pH 6.8) and artificial gastric fluid containing pepsin (pH 6.8) to simulate the dissolution behavior of lutein microcapsule powder in the human gastrointestinal tract.

[0242] Preparation method of artificial gastric juice: Take 16.4 ml of dilute hydrochloric acid, add about 800 ml of water and 10 g of pepsin (the amount of enzyme is adjusted according to the enzyme activity), shake well, and then dilute with water to make a volume of 1000 ml.

[0243] Preparation of artificial intestinal fluid: Dissolve 6.8 g of potassium dihydrogen phosphate in 500 ml of water and adjust the pH to 6.8 with 0.1 mol / L sodium hydroxide solution. Dissolve 10 g of trypsin (adjust the enzyme dosage based on enzyme activity) and 2 g of pancreatic lipase in an appropriate amount of water. Mix the two solutions and dilute with water to a volume of 1000 ml.

[0244] Dissolution content determination method:

[0245] Accurately weigh 120 mg of lutein microcapsule powder and dissolve it in 500 mL of artificial gastric and intestinal fluids, respectively, using a paddle method in a 37°C water bath at 50 rpm. After 30 minutes, 1 hour, 2 hours, 4 hours, and 8 hours, 5 mL of the solution was collected and rapidly filtered through a 0.45 μm aqueous microporous membrane. Cumulative dissolution was determined. Cumulative dissolution = (total lutein dissolved / amount of lutein added) × 100%.

[0246] 3) Flowability evaluation method: Measure the angle of repose of the powder according to GB11986-89 "Measurement of the angle of repose of surfactant powders and particles". If the angle of repose is less than 33°, the powder flowability is "good"; if the angle of repose is ≥ 40°, the powder flowability is "fair"; if the angle of repose is greater than 40°, the powder flowability is "poor".

[0247] Table 10 Effect of different wall materials on the encapsulation efficiency of lutein microcapsule powder

[0248]

[0249] Surface oil content (surface lutein content) is an important indicator for evaluating the microencapsulation efficiency of a product. The lower the surface lutein content, the better the lutein encapsulation effect of the core material.

[0250] Table 10 shows that the wall material containing a mixture of octenylsuccinate monoarabic gum and a composite emulsifier at a mass ratio of 1:0.1 to 0.5 exhibited excellent encapsulation of the core component (lutein), with surface oil content of only 0.1 to 0.18%. The wall material containing octenylsuccinate monoarabic gum and a composite emulsifier at a mass ratio of 0.5:1 exhibited slightly poorer encapsulation of the core component (lutein) than the wall material containing octenylsuccinate monoarabic gum and a composite emulsifier at a mass ratio of 1:0.1 to 0.5. The wall material containing octenylsuccinate monoarabic gum, gum arabic, or gum arabic and a composite emulsifier at a mass ratio of 1:0.5 all exhibited poor encapsulation. It can be seen that the embodiment of the present application can improve the embedding effect of the product, reduce the surface oil content of the product, and improve the quality and stability of the product by introducing a composite emulsifier into octenyl succinate monoarabic gum ester and controlling the mass ratio of octenyl succinate monoarabic gum ester to the composite emulsifier to be 1:0.1~0.5.

[0251] Table 11 Dissolution of lutein microcapsules made with different wall materials in gastric juice at different times

[0252]

[0253] As shown in Table 11, the lutein microcapsules prepared using a mixture of octenylsuccinate monoarabic gum ester and a composite emulsifier at a mass ratio of 1:0.5 as the wall material exhibited a sustained-release time of up to 8 hours, with a prolonged onset of action. The dissolution rate increased uniformly at each time point, demonstrating a smooth release of the lutein microcapsules. 100% of the administered lutein was released, and high therapeutic plasma levels were maintained even after prolonged use. This gentle release significantly improved the bioavailability of lutein. The sustained-release effect of the lutein microcapsule powder prepared by using octenyl succinic acid monoarabic gum ester and a composite emulsifier in a mass ratio of 0.5: 1 as a wall material, or using octenyl succinic acid monoarabic gum ester alone, or using gum arabic, or using gum arabic and a composite emulsifier in a mass ratio of 1: 0.5 as a wall material, is poorer than the sustained-release effect of the lutein microcapsule powder prepared by using octenyl succinic acid monoarabic gum ester and a composite emulsifier in a mass ratio of 1: 0.5 as a wall material, and the bioavailability is relatively poor. It can be seen that the lutein microcapsule powder prepared by using a compound of octenyl succinic acid monoarabic gum ester and a composite emulsifier in a specific ratio as a wall material in the embodiment of the present application has excellent sustained-release performance and greatly improves bioavailability.

[0254] In addition, the dissolution rate of lutein microcapsules with different wall materials in intestinal fluid at different times is similar to the dissolution rate of lutein microcapsules with different wall materials in gastric fluid at different times.

[0255] Table 12 Flowability of lutein microcapsules made from different wall materials

[0256]

[0257] As shown in Table 12, the lutein microcapsule powder obtained by using a compound of octenyl succinate and oligomaltodextrose in a mass ratio of 1: 0.1 to 0.5 as a wall material has good fluidity. The lutein microcapsule powder obtained by using octenyl succinate and the compound in a mass ratio of 5: 1, or using octenyl succinate alone, or using gum arabic, or using gum arabic and a composite emulsifier in a mass ratio of 1: 0.5 as a wall material has average fluidity. It can be seen that the embodiment of the present application is beneficial to improving the fluidity of lutein microcapsule powder by using octenyl succinate and a composite emulsifier in a mass ratio of 1: 0.1 to 0.5 as a wall material.

[0258] Experiment 2: Investigating the effect of emulsifier type on the storage stability and particle size distribution of emulsions

[0259] Each set of experiments employed the same experimental conditions as those used to prepare the lutein microcapsule powder in Example 1. The only difference was that the composite emulsifier used in the lutein microcapsule powder preparation step in Example 1 was replaced with an equivalent mass of each emulsifier listed in Table 13. The storage stability and particle size distribution of each set of third emulsions were tested. The test results are shown in Table 13.

[0260] The test method is as follows: take the same volume of each group of third emulsions, seal them in transparent reagent bottles, and store them in the dark at 25°C. Observe and record the storage stability and particle size distribution of the third emulsions every 5 days for 60 days. The test results are shown in Table 13.

[0261] The particle size and distribution of the emulsion droplets of each group of the third emulsion were measured using a Winner801 laser particle size analyzer.

[0262] Table 13 Storage stability and particle size distribution of the third emulsion prepared with different emulsifiers

[0263]

[0264] The emulsion storage stability is divided into three levels: good, fair, and poor. Good means no stratification, flocculation, or coalescence occurs after 60 days of storage. Fair means stratification, flocculation, or coalescence occurs between 15 and 60 days of storage. Poor means stratification, flocculation, or coalescence occurs between 5 and 10 days of storage.

[0265] Table 13 shows that the third emulsions prepared using either or both of mealworm protein, maltooligosaccharide, or Tween as emulsifiers exhibited average storage stability. Using either mealworm protein, maltooligosaccharide, or Tween as an emulsifier resulted in a larger particle size, approximately 170–200 nm. Using either of these emulsifiers resulted in a smaller particle size, approximately 140–150 nm. Using a composite emulsifier (a combination of mealworm protein, maltooligosaccharide, and Tween) exhibited excellent storage stability and a very small particle size, approximately 20 nm. Therefore, combining mealworm protein, maltooligosaccharide, and Tween not only improves the storage stability of the emulsion but also produces an emulsion with a narrow particle size distribution and smaller particle size, which is beneficial for improving the bioavailability of lutein in the human body.

[0266] Experiment 3: Investigating the effects of different pH values ​​on the storage stability and particle size distribution of emulsions

[0267] Each set of experiments employed the same experimental conditions as those used to prepare the lutein microcapsule powder in Example 1, except that the pH values ​​of the first, second, and third emulsions prepared in Example 1 were adjusted to 3, 4, 5, 6, 7, and 8, respectively. The storage stability and particle size distribution of each set of third emulsions were tested using the same testing methods as in Experiment 2. The test results are shown in Table 14.

[0268] Table 14 Effect of different pH values ​​on storage stability and particle size distribution of emulsion

[0269]

[0270] As shown in Table 14, when preparing the first, second, and third emulsions, controlling the pH value between 3 and 7 can improve the storage stability of the emulsions and produce emulsions with smaller particle sizes. When the pH value is less than 4 or greater than 7, the storage stability of the emulsions is average, and the emulsions have larger particle sizes.

[0271] Experiment 4: Investigating the Effect of Shear Emulsification Process on the Storage Stability and Particle Size Distribution of Emulsions

[0272] Each group of experiments used the same experimental conditions as those used in Example 1 to prepare the lutein microcapsule powder. Only steps S4 to S7 in Example 1 were replaced with "slowly adding the fourth solution to the second solution, controlling the temperature to 25°C and the pH value to 4, and performing shear emulsification at a shear speed of 10,000 r / min to obtain an emulsion". The storage stability and particle size distribution of the prepared emulsion were tested with reference to the test method of Experiment 2. The test results showed that the storage stability of the emulsion was average, and the particle size distribution of the emulsion particles was D 90Around 200nm.

[0273] It can be seen that Example 1 of the present application first splits the second solution into the first aqueous phase and the second aqueous phase, splits the fourth solution into the first oil phase and the second oil phase, and then controls the order and speed of addition of the aqueous phase and the oil phase as well as the shear speed in each step, so that the oil phase and the aqueous phase in the emulsion system are fully mixed during the emulsification process, thereby improving the stability of the emulsion and the uniformity of the particle size distribution, and at the same time improving the preparation efficiency of the emulsion.

[0274] Experiment 5: Investigating the influence of extraction process conditions of Sophora japonica rice powder on the extraction effect

[0275] Each group of experiments adopted the same experimental conditions as the sophora japonica flour in Example 1, except that the extraction solvent of the sophora japonica flour in Example 1 was replaced with an equal volume of various extraction solvents listed in Table 15 below. The following performance index tests were performed on each group of sophora japonica flour obtained. The test results are shown in Table 15:

[0276] Table 15 Effects of different extraction solvents on the extraction of active ingredients (calculated as rutin) from Sophora japonica seeds

[0277]

[0278] Table 15 shows that the three-phase aqueous system formed by combining polyvinyl alcohol, ammonium sulfate, water, and chitosan as the extraction solvent effectively extracted the active ingredient (rutin) from Sophora japonica seeds, achieving the best extraction efficiency and a rutin yield of 312 mg / g. However, the two-phase aqueous system formed by combining polyvinyl alcohol and chitosan, the two-phase aqueous system formed by combining polyvinyl alcohol and ammonium sulfate, or the two-phase aqueous system formed by combining ammonium sulfate and chitosan as the extraction solvent all showed lower extraction efficiency for the active ingredient from Sophora japonica seeds than the extraction solvent prepared with polyvinyl alcohol, ammonium sulfate, water, and chitosan. This indicates that polyvinyl alcohol, ammonium sulfate, and chitosan exhibit a synergistic effect. Furthermore, the extraction solvent prepared with polyvinyl alcohol, ammonium sulfate, water, and chitosan in a mass ratio of 10:5:70:3 effectively extracted the active ingredient (rutin) from Sophora japonica seeds, achieving the highest rutin yield. Therefore, the present application preferably uses an extraction solvent prepared by mixing polyvinyl alcohol, ammonium sulfate, water and chitosan in a mass ratio of 10:5:70:3 to extract the active ingredients of Sophora japonica seeds.

[0279] The above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present application, and should all be included in the scope of protection of the present application.

Claims

1. A composite dietary composition for improving microcirculation, characterized in that: Includes the following components: Freeze-dried fruit and vegetable compound powder, European blueberry concentrate powder, sophora japonica rice powder, (3R,3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and multivitamins; The lutein microcapsule powder comprises the following components: lutein, maltooligosaccharide, octenylsuccinate monoarabic gum ester, vitamin E, silicon dioxide and ascorbyl palmitate; the mass ratio of the octenylsuccinate monoarabic gum ester to the composite emulsifier is 1:0.1-0.5; The preparation steps of the lutein microcapsule powder are as follows: A composite emulsifier is dissolved in water to prepare a first solution, and octenylsuccinate monoarabic gum ester is added to the first solution and mixed to obtain a second solution; the composite emulsifier is a combination of mealworm protein, maltooligosaccharide and Tween; Adding ascorbyl palmitate to oil and heating to dissolve to obtain a third solution; adding lutein, vitamin E, and silicon dioxide to the third solution and dispersing and homogenizing to obtain a fourth solution; Splitting the second solution into a first aqueous phase and a second aqueous phase; Splitting the fourth solution into a first oil phase and a second oil phase; adding the first aqueous phase to the first oil phase at a first speed for shear emulsification to obtain a first emulsion; adding the second aqueous phase to the first emulsion at a second speed for shear emulsification to obtain a second emulsion; adding the second oil phase to the second emulsion at a third speed for shear emulsification to obtain a third emulsion; wherein the first speed is less than the third speed, and the third speed is less than the second speed; in the steps of preparing the first emulsion, the second emulsion, and the third emulsion, the temperature is controlled to be 25-45° C. and the pH value is controlled to be 4-7; The third emulsion is subjected to high-pressure homogenization and spray drying to obtain lutein microcapsule powder.

2. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: The first aqueous phase is added to the first oil phase at a first speed for shear emulsification to obtain a first emulsion, comprising: adding the first aqueous phase to the first oil phase at a rate of 1% to 2% of the total mass of the first system per minute, and performing shear emulsification at a shear speed of 5,000 to 10,000 r / min to obtain a first emulsion; wherein the total mass of the first system refers to the sum of the mass of the first aqueous phase and the mass of the first oil phase; Adding the second aqueous phase to the first emulsion at a second speed for shear emulsification to obtain a second emulsion, comprising: adding the second aqueous phase to the first emulsion at a rate of 3% to 5% of the total mass of the second system per minute, and performing shear emulsification at a shear speed of 10,000 to 25,000 r / min to obtain a second emulsion; wherein the total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion; The second oil phase is added to the second emulsion at a third speed for shear emulsification to obtain a third emulsion, comprising: The second oil phase is added to the second emulsion at a rate of 1% to 3% of the total mass of the third system per minute, and shear emulsification is performed at a shear speed of 10,000 to 25,000 r / min to obtain a third emulsion; wherein the total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion.

3. The composite dietary composition for improving microcirculation according to claim 1, wherein: The lutein microcapsule powder comprises the following components in parts by weight: 10-20 parts of lutein, 1-100 parts of maltooligosaccharide, 10-50 parts of octenylsuccinate monoarabic gum ester, 5-7 parts of vitamin E, 5-10 parts of silicon dioxide, and 1-2 parts of ascorbyl palmitate; The diameter of the lutein microcapsule powder is 1-100 nm.

4. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: The preparation steps of the fermented ginger powder are as follows: Washing, crushing, squeezing and filtering the ginger to obtain ginger juice; After sterilizing the ginger juice, a fermentation strain is added thereto, and the mixture is fermented at 25-35° C. for at least 48 hours to obtain a ginger fermentate; the fermentation strain is at least one of Candida utilis, Acetobacter aceticus, or Aspergillus oryzae; and the mass ratio of the fermentation strain to the ginger juice is 1:100-150; The ginger fermented product is dried to obtain fermented ginger powder.

5. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: The preparation steps of the sophora japonica rice powder are as follows: The dried Sophora japonica seeds are crushed and then subjected to supercritical carbon dioxide enzyme inactivation treatment to obtain an enzyme inactivation product; The enzyme-killed substance is added to an extraction solvent for extraction and separation to obtain an organic phase containing the effective ingredients of Sophora japonica seeds; wherein the extraction solvent comprises the following components in parts by weight: 10-40 parts of polyvinyl alcohol, 5-40 parts of an acidic inorganic salt, 70-90 parts of water, and 2-10 parts of chitosan; The organic phase is concentrated under reduced pressure to precipitate crystals to obtain sophora japonica flour.

6. The composite dietary composition for improving microcirculation according to claim 5, characterized in that: The solid-liquid ratio of the enzyme-inactivating substance to the extraction solvent is 1:6-10; And / or, the extraction conditions are: extraction time of 10-15 minutes, extraction temperature of 25-70°C, and pH value of 4-6.

7. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: The composite dietary composition comprises the following components in parts by weight: 30-40 parts of freeze-dried fruit and vegetable composite powder, 1-3 parts of European blueberry concentrated powder, 4-7 parts of sophora japonica rice powder, 0.1-5 parts of (3R,3'R)-dihydroxy-β-carotene, 0.1-0.5 parts of lutein microcapsule powder, 30-40 parts of matcha powder, 0.01-2 parts of fermented ginger powder, and 2-7 parts of multivitamins; The freeze-dried fruit and vegetable composite powder comprises at least one of kale powder, hawthorn powder, pomegranate powder, blackcurrant powder, acerola cherry powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder or bitter melon powder; The vitamin complex includes at least three of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D and vitamin E.

8. The method for preparing the composite dietary composition for improving microcirculation according to claim 1, wherein: include: The freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, sophora japonica rice powder, (3R, 3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and complex vitamins are mixed with a tablet flow aid, and tablets are formed.

Images