Multi-target functional composition as well as preparation method and application thereof

Abstract

The invention relates to a multi-target-point functional composition as well as a preparation method and application thereof. The multi-target-point functional composition comprises ascorbyl tetraisopalmitate, a sea-buckthorn extract, phytol, an oil-soluble collagen solution and an oil-soluble polypeptide solution, the oil-soluble collagen liquid is a water-in-oil type solution with a reverse micelle structure and is prepared from phospholipid, an oil phase and a water phase, and the water phase comprises soluble collagen; the oil-soluble polypeptide liquid is a water-in-oil type solution with a reverse micelle structure, preparation raw materials of the oil-soluble polypeptide liquid comprise phospholipid, an oil phase and a water phase, and the water phase comprises polypeptide. The five components in the multi-target-point functional composition interact with one another and have a synergistic effect, and the anti-wrinkle, tightening and brightening effects of the composition are jointly improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a multi-target efficacy composition and a preparation method and application thereof. Background Art

[0002] As people pay more and more attention to appearance and beauty, eye care has become an important part of skin care. Eye skin is one of the thinnest and most delicate parts of the human body, and is prone to fine lines, dark circles, eye bags and other problems. Therefore, consumers' demand for care products that can effectively improve eye skin problems continues to increase, driving the rapid development of the eye essence oil market. Traditional eye care products often have problems with poor performance, such as poor transdermal absorption of water-soluble active ingredients.

[0003] CN118634168A discloses a whitening and anti-aging composition, a whitening and anti-aging cream and a preparation method thereof, wherein the whitening and anti-aging composition comprises 0.1-1% glabridin, 0.1-10% rhamnosylsilanol, 0.1-10% yarrow extract, 0.1-40% peony root extract, 0.1-10% acetylated sodium hyaluronate, 20-24% moisturizer and the remainder water. The components of the composition provided by the invention cooperate with each other to give full play to their functions, so that the whitening and anti-aging effects of the composition are significant, the skin condition and texture are improved, the combination is reasonable, and the whitening, anti-aging and wrinkle removal effects are excellent, but the anti-wrinkle and anti-aging effects cannot be improved from multiple dimensions.

[0004] Therefore, how to provide a multi-target efficacy composition with good brightening, anti-wrinkle, and firming effects and high stability has become a problem that needs to be solved urgently. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention aims to provide a multi-target efficacy composition and its preparation method and application. The five components in the multi-target efficacy composition interact with each other and synergize to jointly improve the anti-wrinkle, firming and brightening effects of the composition.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a multi-target efficacy composition, which comprises ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid.

[0008] The oil-soluble collagen solution is a water-in-oil type solution with a reverse micelle structure. The raw materials for preparing the oil-soluble collagen solution include phospholipids, an oil phase and an aqueous phase, and the aqueous phase includes soluble collagen.

[0009] The oil-soluble polypeptide liquid is a water-in-oil type solution with a reverse micelle structure, and its preparation raw materials include phospholipids, an oil phase and an aqueous phase, and the aqueous phase includes polypeptides.

[0010] Ascorbyl tetraisopalmitate is a derivative of vitamin C, which has good antioxidant, melanin inhibition and collagen promotion effects. Sea buckthorn extract is an active ingredient extracted from sea buckthorn, rich in vitamins, flavonoids, fatty acids and other nutrients, which can help resist free radical damage to the skin, and has whitening, anti-inflammatory and skin metabolism promoting effects. Phytol is a compound extracted from plant chlorophyll, which has antioxidant, anti-inflammatory and skin barrier repair promoting effects.

[0011] Oil-soluble collagen liquid and oil-soluble polypeptide liquid successfully apply water-soluble soluble collagen and polypeptide to the oil-soluble system, protecting the activity and stability of the active ingredients soluble collagen and polypeptide, improving the permeability and transdermal absorption effect of the composition, and solving the skin problems around the eyes from multiple targets. Dispersing soluble collagen and polypeptide in two systems can better improve the encapsulation of soluble collagen and polypeptide by oil-soluble collagen liquid and oil-soluble polypeptide liquid, and increase the content of active ingredients in the composition.

[0012] The composition includes ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid. The five components interact with each other and synergistically enhance the anti-wrinkle, firming and brightening effects of the composition.

[0013] Preferably, the aqueous phase in the oil-soluble collagen solution further comprises a pH regulator.

[0014] Preferably, the pH adjuster includes disodium hydrogen phosphate and sodium dihydrogen phosphate.

[0015] Preferably, the polypeptide in the oil-soluble polypeptide liquid includes any one or a combination of at least two of acetyl tetrapeptide-5, acetyl hexapeptide-8, palmitoyl tripeptide-1, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl pentapeptide-3 or palmitoyl tripeptide-5.

[0016] Preferably, the polypeptide comprises dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1 and palmitoyl pentapeptide-3.

[0017] Dipeptide diaminobutyryl benzylamide diacetate is a synthetic polypeptide that simulates snake venom serum. It blocks the binding of acetylcholine to the receptors by acting on the muscle nicotinic acetylcholine receptors of the postsynaptic membrane, resulting in receptor closure, thereby relaxing the muscles and achieving the effect of wrinkle removal; Palmitoyl tripeptide-1 can transmit signals to skin cells, stimulate the synthesis of collagen, and has the effects of improving skin elasticity, moisturizing the skin, and anti-inflammatory and antibacterial; Palmitoyl pentapeptide-3 activates fibroblasts, promotes the production of collagen, fibronectin and hyaluronic acid, reduces wrinkles and increases skin firmness. The three interact with each other and synergize, and together as the main active ingredients in the oil-soluble polypeptide liquid, they can further enhance the anti-wrinkle and firming effects of the multi-target efficacy composition.

[0018] Preferably, the phospholipids in the oil-soluble collagen liquid and the oil-soluble polypeptide liquid each independently include lecithin.

[0019] Preferably, the oil phases in the oil-soluble collagen liquid and the oil-soluble polypeptide liquid independently include an oily antioxidant and an oily solvent.

[0020] Preferably, the oily antioxidant comprises tocopheryl acetate.

[0021] Preferably, the oily solvent includes any one of isopropyl myristate, caprylic / capric triglyceride, and isopropyl palmitate, or a combination of at least two thereof.

[0022] Preferably, the multi-target efficacy composition comprises, by weight, 0.1-2 parts of ascorbyl tetraisopalmitate, 0.1-3 parts of seabuckthorn extract, 0.1-1 parts of phytol, 0.05-5 parts of oil-soluble collagen liquid and 0.05-5 parts of oil-soluble polypeptide liquid.

[0023] Among them, the specific point values ​​in 0.1~2 parts can be selected from 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, etc., the specific point values ​​in 0.1~3 parts can be selected from 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, etc., the specific point values ​​in 0.1~1 parts can be selected from 0.1 part, 0.3 part, 0.5 part, 0.7 part, 0.9 part, 1 part, etc., 0.05~5 parts can be selected from 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0024] Preferably, in terms of mass percentage, the raw materials for preparing the oil-soluble collagen solution include 65-75% oily solvent, 25-33% phospholipids, 1-1.5% water, 0.5-1% oily antioxidant, 0.01-0.05% soluble collagen, and 0.001-0.003% pH adjuster.

[0025] Among them, the specific point values ​​in 65-75% can be selected from 65%, 67%, 69%, 71%, 73%, 75%, etc., the specific point values ​​in 25-33% can be selected from 25%, 27%, 29%, 31%, 33%, etc., the specific point values ​​in 1-1.5% can be selected from 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, etc., the specific point values ​​in 0.5-1% can be selected from The specific point values ​​in the range of 0.01-0.05% can be selected from 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, etc. The specific point values ​​in the range of 0.001-0.003% can be selected from 0.001%, 0.0015%, 0.002%, 0.0025%, 0.003%, etc.

[0026] Preferably, in terms of mass percentage, the raw materials for preparing the oil-soluble polypeptide liquid include 65-75% oily solvent, 25-33% phospholipids, 1-1.5% water, 0.5-1% oily antioxidant, and 0.001-0.1% polypeptide.

[0027] Among them, the specific point values ​​in 65-75% can be selected from 65%, 67%, 69%, 71%, 73%, 75%, etc., the specific point values ​​in 25-33% can be selected from 25%, 27%, 29%, 31%, 33%, etc., the specific point values ​​in 1-1.5% can be selected from 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, etc., the specific point values ​​in 0.5-1% can be selected from 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc., and the specific point values ​​in 0.001-0.1% can be selected from 0.001%, 0.01%, 0.03%, 0.05%, 0.07%, 0.09%, 0.1%, etc.

[0028] Preferably, the oil-soluble collagen solution is prepared by a preparation method comprising the following steps:

[0029] (1) The oil phase and phospholipids are mixed to form a mixed solution I.

[0030] (2) Adding the aqueous phase dropwise into the mixed solution I and stirring to form an oil-soluble collagen solution with a reverse micelle structure.

[0031] Preferably, the mixing temperature in step (1) is 50-80° C., and the mixing time is 0.5-4 h.

[0032] Preferably, the stirring speed in step (2) is 100 to 1000 rpm, the stirring temperature is 20 to 40° C., and the stirring time is 0.5 to 3 h.

[0033] Among them, the specific point values ​​in 50-80℃ can be selected from 50℃, 55℃, 60℃, 65℃, 70℃, 75℃, 80℃, etc., the specific point values ​​in 20-40℃ can be selected from 20℃, 25℃, 30℃, 35℃, 40℃, etc., the specific point values ​​in 0.5-4h can be selected from 0.5h, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, etc., the specific point values ​​in 0.5-3h can be selected from 0.5h, 1h, 1.5h, 2h, 2.5h, 3h, etc., the specific point values ​​in 100-1000rpm can be selected from 100rpm, 300rpm, 500rpm, 700rpm, 900rpm, 1000rpm, etc.

[0034] Preferably, the oil-soluble polypeptide liquid is prepared by a preparation method comprising the following steps:

[0035] (a) The oil phase and phospholipids are mixed to form a mixed solution II.

[0036] (b) adding the aqueous phase dropwise into the mixed solution II and stirring to form an oil-soluble polypeptide solution having a reverse micelle structure.

[0037] Preferably, the mixing temperature in step (a) is 50 to 80° C., and the mixing time is 0.5 to 4 h;

[0038] Preferably, the stirring speed in step (b) is 100 to 1000 rpm, the stirring temperature is 20 to 40° C., and the stirring time is 0.5 to 3 h.

[0039] Among them, the specific point values ​​in 50-80℃ can be selected from 50℃, 55℃, 60℃, 65℃, 70℃, 75℃, 80℃, etc., the specific point values ​​in 20-40℃ can be selected from 20℃, 25℃, 30℃, 35℃, 40℃, etc., the specific point values ​​in 0.5-4h can be selected from 0.5h, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, etc., the specific point values ​​in 0.5-3h can be selected from 0.5h, 1h, 1.5h, 2h, 2.5h, 3h, etc., the specific point values ​​in 100-1000rpm can be selected from 100rpm, 300rpm, 500rpm, 700rpm, 900rpm, 1000rpm, etc.

[0040] In a second aspect, the present invention provides a method for preparing the multi-target efficacy composition as described in the first aspect, the preparation method comprising the following steps:

[0041] Ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid are mixed at 20-40° C. to obtain a multi-target efficacy composition.

[0042] Among them, the specific point values ​​between 20 and 40°C can be selected from 20°C, 25°C, 30°C, 35°C, 40°C, etc.

[0043] In a third aspect, the present invention provides a use of the multi-target efficacy composition as described in the first aspect in cosmetics.

[0044] The numerical range described in the present invention not only includes the point values ​​listed above, but also includes any point values ​​between the above numerical ranges that are not listed. Due to space limitations and for the sake of simplicity, the present invention no longer exhaustively lists the specific point values ​​included in the range.

[0045] Compared with the prior art, the present invention has the following beneficial effects:

[0046] The present invention creatively mixes ascorbic acid tetraisopalmitate, seabuckthorn extract, phytol, oil-soluble collagen solution and oil-soluble polypeptide solution, and the components synergize to enhance the effects of the composition in terms of anti-oxidation, whitening and lightening, anti-wrinkle and firming.

[0047] Oil-soluble collagen liquid and oil-soluble polypeptide liquid successfully apply water-soluble soluble collagen and polypeptide to oil-soluble system, without adding emulsifier, which can better protect the activity and stability of active ingredients soluble collagen and polypeptide, improve the penetration and transdermal absorption effect of the composition, and solve skin problems with multiple targets;

[0048] Furthermore, when the polypeptides in the oil-soluble polypeptide liquid include dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1 and palmitoyl pentapeptide-3, the three work synergistically, enabling the multi-target efficacy composition to better exert the effects of antioxidant, brightening and firming. DETAILED DESCRIPTION

[0049] In order to further illustrate the technical means and effects of the present invention, the technical scheme of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments. The sources of the components / raw materials in the following embodiments are shown in Table 1:

[0050] Table 1

[0051]

[0052] Preparation Example 1-1

[0053] This preparation example provides an oil-soluble collagen liquid with a reverse micelle structure. The raw materials for preparing the oil-soluble collagen liquid include 72% isopropyl myristate, 0.7% tocopherol acetate, 26% lecithin, 1.247% water, 0.05% soluble collagen, 0.002% disodium hydrogen phosphate, and 0.001% sodium dihydrogen phosphate, in terms of mass percentage.

[0054] Preparation method:

[0055] (1) mixing tocopherol acetate, isopropyl myristate, and lecithin at 70° C. for 2 h to obtain a mixed solution;

[0056] (2) mixing water, soluble collagen, disodium hydrogen phosphate, and sodium dihydrogen phosphate to obtain an aqueous phase;

[0057] (3) Add the aqueous phase dropwise into the mixed solution of step (1) and stir to form an oil-soluble collagen solution with a reverse micelle structure. The stirring temperature is 25° C., the time is 1 h, and the rotation speed is 250 rpm.

[0058] Preparation Example 1-2

[0059] This preparation example provides an oil-soluble collagen liquid with a reverse micelle structure. The raw materials for preparing the oil-soluble collagen liquid include 67.5% isopropyl myristate, 1% tocopherol acetate, 30% lecithin, 1.468% water, 0.03% soluble collagen, 0.001% disodium hydrogen phosphate, and 0.001% sodium dihydrogen phosphate, in terms of mass percentage.

[0060] Preparation method:

[0061] (1) mixing tocopherol acetate, isopropyl myristate and lecithin at 55° C. for 3 h to obtain a mixed solution;

[0062] (2) mixing water, soluble collagen, disodium hydrogen phosphate, and sodium dihydrogen phosphate to obtain an aqueous phase;

[0063] (3) Add the aqueous phase dropwise into the mixed solution of step (1), and stir to form an oil-soluble collagen solution with a reverse micelle structure. The stirring temperature is 20° C., the time is 2 h, and the rotation speed is 400 rpm.

[0064] Preparation Example 1-3

[0065] This preparation example provides an oil-soluble collagen liquid with a reverse micelle structure. The raw materials for preparing the oil-soluble collagen liquid include 65% isopropyl myristate, 0.5% tocopherol acetate, 33% lecithin, 1.477% water, 0.02% soluble collagen, 0.001% disodium hydrogen phosphate, and 0.002% sodium dihydrogen phosphate, in terms of mass percentage.

[0066] Preparation method:

[0067] (1) mixing tocopherol acetate, isopropyl myristate, and lecithin at 80° C. for 1 h to obtain a mixed solution;

[0068] (2) mixing water, soluble collagen, disodium hydrogen phosphate, and sodium dihydrogen phosphate to obtain an aqueous phase;

[0069] (3) Add the aqueous phase dropwise into the mixed solution of step (1), and stir to form an oil-soluble collagen solution with a reverse micelle structure. The stirring temperature is 30° C., the time is 1 h, and the rotation speed is 600 rpm.

[0070] Preparation Example 2-1

[0071] This preparation example provides an oil-soluble polypeptide liquid with a reverse micelle structure. The raw materials for preparing the oil-soluble polypeptide liquid include 72% isopropyl myristate, 0.7% tocopherol acetate, 26% lecithin, 1.285% water, 0.005% dipeptide diaminobutyryl benzylamide diacetate, 0.005% palmitoyl tripeptide-1, and 0.005% palmitoyl pentapeptide-3, in terms of mass percentage.

[0072] Preparation method:

[0073] (1) mixing tocopherol acetate, isopropyl myristate, and lecithin at 70° C. for 2 h to obtain a mixed solution;

[0074] (2) mixing water, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1, and palmitoyl pentapeptide-3 to obtain an aqueous phase;

[0075] (3) Add the aqueous phase dropwise into the mixed solution of step (1) and stir to form an oil-soluble collagen solution with a reverse micelle structure. The stirring temperature is 25° C., the time is 1 h, and the rotation speed is 250 rpm.

[0076] Preparation Example 2-2

[0077] This preparation example provides an oil-soluble polypeptide liquid with a reverse micelle structure. The raw materials for preparing the oil-soluble polypeptide liquid include 67.5% isopropyl myristate, 1% tocopherol acetate, 30% lecithin, 1.45% water, 0.01% dipeptide diaminobutyryl benzylamide diacetate, 10.02% palmitoyl tripeptide, and 30.02% palmitoyl pentapeptide, in terms of mass percentage.

[0078] Preparation method:

[0079] (1) mixing tocopherol acetate, isopropyl myristate and lecithin at 55° C. for 3 h to obtain a mixed solution;

[0080] (2) mixing water, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1, and palmitoyl pentapeptide-3 to obtain an aqueous phase;

[0081] (3) Add the aqueous phase dropwise into the mixed solution of step (1), and stir to form an oil-soluble collagen solution with a reverse micelle structure. The stirring temperature is 20° C., the time is 2 h, and the rotation speed is 400 rpm.

[0082] Preparation Example 2-3

[0083] This preparation example provides an oil-soluble polypeptide liquid with a reverse micelle structure. The raw materials for preparing the oil-soluble polypeptide liquid include 65% isopropyl myristate, 0.5% tocopherol acetate, 33% lecithin, 1.41% water, 0.04% dipeptide diaminobutyryl benzylamide diacetate, 10.02% palmitoyl tripeptide, and 30.03% palmitoyl pentapeptide, in terms of mass percentage.

[0084] Preparation method:

[0085] (1) mixing tocopherol acetate, isopropyl myristate, and lecithin at 80° C. for 1 h to obtain a mixed solution;

[0086] (2) mixing water, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1, and palmitoyl pentapeptide-3 to obtain an aqueous phase;

[0087] (3) Add the aqueous phase dropwise into the mixed solution of step (1), and stir to form an oil-soluble collagen solution with a reverse micelle structure. The stirring temperature is 30° C., the time is 1 h, and the rotation speed is 600 rpm.

[0088] Preparation Example 2-4

[0089] This preparation example provides an oil-soluble polypeptide liquid with a reverse micelle structure, which differs from Preparation Example 2-1 only in that 0.005% of dipeptide diaminobutyryl benzylamide diacetate, 0.005% of palmitoyl tripeptide-1, and 0.005% of palmitoyl pentapeptide-3 are replaced with 0.0075% of dipeptide diaminobutyryl benzylamide diacetate and 0.0075% of palmitoyl tripeptide-1; accordingly, palmitoyl pentapeptide-3 is not added in step (2) of the preparation method.

[0090] Preparation Example 2-5

[0091] This preparation example provides an oil-soluble polypeptide liquid with a reverse micelle structure, which differs from Preparation Example 2 only in that 0.005% of dipeptide diaminobutyryl benzylamide diacetate, 0.005% of palmitoyl tripeptide-1, and 0.005% of palmitoyl pentapeptide-3 are replaced with 0.0075% of dipeptide diaminobutyryl benzylamide diacetate and 0.0075% of palmitoyl pentapeptide-3; accordingly, palmitoyl tripeptide-1 is not added in step (2) of the preparation method.

[0092] Preparation Example 2-6

[0093] This preparation example provides an oil-soluble polypeptide liquid with a reverse micelle structure, which differs from Preparation Example 2 only in that 0.005% of dipeptide diaminobutyryl benzylamide diacetate, 0.005% of palmitoyl tripeptide-1, and 0.005% of palmitoyl pentapeptide-3 are replaced with 0.0075% of palmitoyl tripeptide-1 and 0.0075% of palmitoyl pentapeptide-3; accordingly, dipeptide diaminobutyryl benzylamide diacetate is not added in step (2) of the preparation method.

[0094] Comparative Preparation Example 1

[0095] This comparative preparation example provides a collagen emulsion. The raw materials for preparing the collagen emulsion include 72% isopropyl myristate, 0.7% tocopherol acetate, 10% polyoxyethylene 40 hydrogenated castor oil, 16% Tween 20, 1.247% water, 0.05% soluble collagen, 0.002% disodium hydrogen phosphate, and 0.001% sodium dihydrogen phosphate, in terms of mass percentage.

[0096] Preparation method:

[0097] Water, soluble collagen, disodium hydrogen phosphate and sodium dihydrogen phosphate are mixed to form an aqueous phase; isopropyl myristate, tocopherol acetate, polyoxyethylene 40 hydrogenated castor oil and Tween 20 are mixed to form an oil phase; the aqueous phase and the oil phase are mixed, stirred at 60° C. for 10 minutes, and homogenized to obtain a collagen emulsion.

[0098] Comparative Preparation Example 2

[0099] This preparation example provides a polypeptide emulsion. The raw materials for preparing the polypeptide emulsion include, by weight percentage, 72% isopropyl myristate, 0.7% tocopherol acetate, 10% polyoxyethylene 40 hydrogenated castor oil, 16% Tween 20, 1.285% water, 0.005% dipeptide diaminobutyryl benzylamide diacetate, 0.005% palmitoyl tripeptide-1, and 0.005% palmitoyl pentapeptide-3;

[0100] Preparation method:

[0101] Water, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1, and palmitoyl pentapeptide-3 are mixed to form an aqueous phase; isopropyl myristate, tocopherol acetate, polyoxyethylene 40 hydrogenated castor oil, and Tween 20 are mixed to form an oil phase; the aqueous phase and the oil phase are mixed, stirred at 60° C. for 10 minutes, and homogenized to obtain a polypeptide emulsion.

[0102] Example 1

[0103] This embodiment provides a multi-target efficacy composition, which includes, by weight, 1 part of ascorbyl tetraisopalmitate, 1.5 parts of seabuckthorn extract, 0.5 parts of phytol, 2 parts of the oil-soluble collagen solution prepared in Preparation Example 1-1, and 2 parts of the oil-soluble polypeptide solution prepared in Preparation Example 2-1;

[0104] Preparation method:

[0105] Ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid are mixed at 25°C to obtain the product.

[0106] Example 2

[0107] This embodiment provides a multi-target efficacy composition, which includes, by weight, 1.5 parts of ascorbyl tetraisopalmitate, 1 part of seabuckthorn extract, 1 part of phytol, 1 part of the oil-soluble collagen solution prepared in Preparation Example 1-2, and 3 parts of the oil-soluble polypeptide solution prepared in Preparation Example 2-2;

[0108] Preparation method:

[0109] Ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid are mixed at 30°C to obtain the product.

[0110] Example 3

[0111] This embodiment provides a multi-target efficacy composition, which includes, by weight, 0.5 parts of ascorbyl tetraisopalmitate, 3 parts of seabuckthorn extract, 0.5 parts of phytol, 4 parts of the oil-soluble collagen solution prepared in Preparation Example 1-3, and 2 parts of the oil-soluble polypeptide solution prepared in Preparation Example 2-3;

[0112] Preparation method:

[0113] Ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid are mixed at 20°C to obtain the product.

[0114] Example 4

[0115] This example provides a multi-target efficacy composition, which differs from Example 1 only in that the oil-soluble polypeptide liquid prepared in Preparation Example 2-1 is replaced with an oil-soluble polypeptide liquid prepared in Preparation Example 2-4 of equal mass.

[0116] Example 5

[0117] This example provides a multi-target efficacy composition, which differs from Example 1 only in that the oil-soluble polypeptide liquid prepared in Preparation Example 2-1 is replaced with an oil-soluble polypeptide liquid prepared in Preparation Example 2-5 of equal mass.

[0118] Example 6

[0119] This example provides a multi-target efficacy composition, which differs from Example 1 only in that the oil-soluble polypeptide liquid prepared in Preparation Example 2-1 is replaced with an oil-soluble polypeptide liquid prepared in Preparation Example 2-6 of equal mass.

[0120] Comparative Example 1

[0121] This comparative example provides a multi-target efficacy composition, which is different from Example 1 only in that ascorbyl tetraisopalmitate is not included in the composition, and the weight proportions of the other components remain unchanged.

[0122] Comparative Example 2

[0123] This comparative example provides a multi-target efficacy composition, which is different from Example 1 only in that the composition does not include seabuckthorn extract, and the weight proportions of the other components remain unchanged.

[0124] Comparative Example 3

[0125] This comparative example provides a multi-target efficacy composition, which differs from Example 1 only in that phytol is not included in the composition, and the weight proportions of the other components remain unchanged.

[0126] Comparative Example 4

[0127] This comparative example provides a multi-target efficacy composition, which differs from Example 1 only in that the composition does not include an oil-soluble collagen solution, and the weight proportions of the other components remain unchanged.

[0128] Comparative Example 5

[0129] This comparative example provides a multi-target efficacy composition, which differs from Example 1 only in that the composition does not include an oil-soluble polypeptide liquid, and the weight proportions of the other components remain unchanged.

[0130] Comparative Example 6

[0131] This comparative example provides a multi-target efficacy composition, which differs from Example 1 only in that the oil-soluble collagen liquid prepared in Preparation Example 1-1 is replaced by an equal mass of collagen emulsion prepared in Comparative Preparation Example 1, and the oil-soluble polypeptide liquid prepared in Preparation Example 2-1 is replaced by an equal mass of polypeptide emulsion prepared in Comparative Preparation Example 2.

[0132] Application Example 1

[0133] This application example provides an essential oil, the formula of which is shown in the following table:

[0134] Table 2

[0135] raw material Mass percentage The multi-target efficacy composition in Example 1 5％ Sunflower Seed Oil 10％ Jojoba Seed Oil to 100% Caprylic / capric triglyceride 30％ olive oil 10％

[0136] Preparation method: At 25°C, stir and mix the above components evenly, and filter to obtain the product.

[0137] Application Examples 2 to 6

[0138] Application Examples 2 to 6 each provide an essential oil, which differs from Application Example 1 only in that the multi-target efficacy composition in Example 1 is replaced by the multi-target efficacy composition in Examples 2 to 6, and the other components remain unchanged.

[0139] Comparative Application Examples 1 to 6

[0140] Comparative Application Examples 1 to 6 each provide an essential oil, which differs from Application Example 1 only in that the multi-target efficacy composition in Example 1 is replaced by the multi-target efficacy composition in Comparative Examples 1 to 6, and the other components remain unchanged.

[0141] Test Example 1

[0142] Evaluation of DPPH free radical scavenging ability:

[0143] The ability to scavenge DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) free radicals reflects the antioxidant capacity of a substance to a certain extent. The greater the free radical scavenging rate, the stronger the antioxidant capacity and the stronger the anti-aging ability. Therefore, the anti-aging effect of the multi-target efficacy composition can be judged by studying its ability to scavenge DPPH free radicals.

[0144] The multi-target efficacy composition prepared in Examples 1-6 and Comparative Examples 1-6 was diluted with DMSO to a sample with a mass fraction of 2%, and the following test was performed:

[0145] Add 2.0 mL of 45.8 mg / L DPPH test solution and the sample to be tested to the test tube, add anhydrous ethanol to the total volume to 3 mL, shake well, and react in the dark for 30 minutes. Then use a 1 cm cuvette to measure the absorbance at a wavelength of 517 nm, which is recorded as A. 10 ; Add 2 mL of anhydrous ethanol and the corresponding volume of the sample to be tested to the test tube, and add anhydrous ethanol to make up the total volume to 3 mL. The measured absorbance is recorded as A 11 ; Add 2 mL of DPPH test solution and 1 mL of anhydrous ethanol to the test tube, and the measured absorbance is recorded as A 12 , calculate the DPPH free radical scavenging rate (Y') of the test solution.

[0146] The calculation formula of DPPH free radical scavenging rate is:

[0147]

[0148] Where A 10 A is the absorbance value of the system after the sample to be tested removes DPPH,11 A is the absorbance value of the system after removing DPPH from the sample blank control. 12 It is the absorbance value of the reaction system before adding the sample to be tested.

[0149] The test results are shown in the following table:

[0150] Table 3

[0151] sample DPPH free radical scavenging rate (%) Example 1 72 Example 2 69 Example 3 68 Example 4 60 Example 5 58 Example 6 59 Comparative Example 1 42 Comparative Example 2 41 Comparative Example 3 39 Comparative Example 4 40 Comparative Example 5 38 Comparative Example 6 36

[0152] From the data of Example 1 and Comparative Examples 1 to 5, it can be seen that when any one of ascorbyl tetraisopalmitate, seabuckthorn extract, phytol, oil-soluble collagen liquid or oil-soluble polypeptide liquid is missing in the multi-target efficacy composition, the DPPH free radical scavenging ability of the composition is reduced to varying degrees, indicating that ascorbyl tetraisopalmitate, seabuckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid can jointly improve the antioxidant capacity of the composition. From the data of Example 1 and Comparative Example 6, it can be seen that compared with the method of adding an emulsifier to disperse the oil phase and the water phase, the oil-soluble collagen liquid and the oil-soluble polypeptide liquid in Example 1 form an oil-in-water type solution with a reverse micelle structure, which can further protect the stability and activity of the water-soluble components in the oil-in-water type solution, and further improve the overall antioxidant effect of the composition. It can be seen from the data of Examples 1 and 4 to 6 that when the polypeptides in the oil-soluble polypeptide liquid simultaneously include dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1 and palmitoyl pentapeptide-3, the antioxidant capacity of the composition is further improved, indicating that the three have a significant synergistic effect in antioxidant properties.

[0153] Test Example 2

[0154] Brightening and whitening effect evaluation:

[0155] (1) Test subjects: 120 healthy women (30-45 years old), divided into 12 groups, each with 10 people; each group used the products of Application Examples 1-6 and Comparative Application Examples 1-6.

[0156] (2) Test environment: temperature 20℃~22℃, humidity 40%~60%.

[0157] (3) Testing instrument: Delfin SkinColorCatch (Finland).

[0158] (4) Test method: After cleansing and toning in the morning and evening, apply an appropriate amount of the essence oil of the application example and the comparative application example around the eyes, spread evenly and massage until absorbed, and continue to use for 28 days.

[0159] (5) Test indicators: The L* value (brightness) and ITA° value (color individual type angle, representing the overall change in chromaticity) of the eye skin color were tested before use (D0) and 28 days after use (D28).

[0160] Among them, the skin color under different ITA° value ranges is shown in the following table:

[0161] Table 4

[0162]

[0163]

[0164] The test results of the essential oils of each application example and comparative application example are shown in the following table:

[0165] Table 5

[0166]

[0167] Test Example 3

[0168] Anti-wrinkle and firming effect evaluation:

[0169] (1) Testers: 120 healthy women (aged 30-45) from the same group as in Test Example 2 were selected and divided into 12 groups, each with 10 participants. Each group used the products of Application Examples 1-6 and Comparative Application Examples 1-6.

[0170] (2) Test environment: The same as that of Test Example 2.

[0171] (3) Testing instruments: Visia-CR is used for facial image acquisition and wrinkle analysis, skin elasticity measuring instrument MPA580 is used for testing skin elasticity, and skin elasticity measuring instrument MPA580 is used for testing skin firmness.

[0172] (4) Test method: Same as the test method in Test Example 2.

[0173] (5) Test indicators: change rate of eye wrinkle area, change rate of skin elasticity R2, change rate of skin firmness; change rate = (measurement value after use (D28) - measurement value before use (D0)) / measurement value before use (D0) × 100%.

[0174] The test results of the essential oils of each application example and comparative application example are shown in the following table:

[0175] Table 6

[0176] sample Change rate of eye wrinkle area Skin elasticity R2 change rate Skin firmness change rate Application Example 1 -20 29 -56 Application Example 2 -19 26 -54 Application Example 3 -18 27 -55 Application Example 4 -15 25 -50 Application Example 5 -16 24 -49 Application Example 6 -16 23 -50 Comparative application example 1 -12 16 -39 Comparative Application Example 2 -13 14 -35 Comparative Application Example 3 -11 15 -32 Comparative Application Example 4 -12 13 -33 Comparative Application Example 5 -10 14 -35 Comparative Application Example 6 -10 12 -30

[0177] It can be seen from the data in Table 5 and Table 6 that the multi-target efficacy composition of the present invention has excellent whitening, brightening, anti-wrinkle and skin tightening effects.

[0178] It can be seen from the data of Application Example 1 and Comparative Application Examples 1 to 5 that when any one of ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid or oil-soluble polypeptide liquid is missing in the multi-target efficacy composition, the whitening, brightening, anti-wrinkle and skin tightening effects of the essence oil are reduced to varying degrees, indicating that ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid can synergistically improve the whitening, brightening, anti-wrinkle and skin tightening effects of the composition. It can be seen from the data of Application Example 1 and Comparative Application Example 6 that compared with the method of adding an emulsifier to disperse the oil phase and the water phase, the present invention forms an oil-in-water type solution with a reverse micelle structure through oil-soluble collagen liquid and oil-soluble polypeptide liquid, which can better protect the stability and activity of the water-soluble components in the oil-in-water type solution, so that the water-soluble components and the oil-soluble components can better play a synergistic role. It can be seen from the data of Application Examples 1 and Application Examples 4 to 6 that when the polypeptides in the oil-soluble polypeptide liquid simultaneously include dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1 and palmitoyl pentapeptide-3, the whitening, brightening, anti-wrinkle and skin firming effects of the composition are all improved to varying degrees, indicating that the three have significant synergistic effects in brightening, anti-wrinkle and firming.

[0179] Test Example 4

[0180] Stability test:

[0181] The multi-target efficacy compositions prepared in Examples 1 to 6 and Comparative Examples 1 to 6 were subjected to stability tests. The test method was to take 10 g of each sample of the multi-target efficacy composition and put it into a test tube. After sealing, the sample was placed at 50° C. for 30 days to observe changes in appearance. Another sample was irradiated with a cool fluorescent lamp at 25° C. for 24 hours with a light intensity of 2000 Lux to observe changes in appearance. The results are shown in the following table.

[0182] Table 7

[0183]

[0184]

[0185] It can be seen from the data in Table 7 that compared with the traditional method of adding an emulsifier to disperse the oil phase and the water phase, the present invention forms an oil-in-water type solution with a reverse micelle structure, which can not only better maintain the activity of the water-soluble components in the oil-in-water type solution, but also further improve the long-term stability of the multi-target efficacy composition.

[0186] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0187] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0188] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A multi-target efficacy composition, characterized in that: The multi-target efficacy composition comprises ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid; The oil-soluble collagen solution is a water-in-oil type solution with a reverse micelle structure, and its preparation raw materials include phospholipids, an oil phase and an aqueous phase, and the aqueous phase includes soluble collagen; The oil-soluble polypeptide liquid is a water-in-oil type solution with a reverse micelle structure, and its preparation raw materials include phospholipids, an oil phase and an aqueous phase, and the aqueous phase includes polypeptides.

2. The multi-target efficacy composition according to claim 1, characterized in that: The water phase in the oil-soluble collagen solution also includes a pH regulator.

3. The multi-target efficacy composition according to claim 1 or 2, characterized in that: The polypeptide in the oil-soluble polypeptide liquid includes any one of acetyl tetrapeptide-5, acetyl hexapeptide-8, palmitoyl tripeptide-1, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl pentapeptide-3 or palmitoyl tripeptide-5, or a combination of at least two thereof; Preferably, the polypeptide comprises dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-1 and palmitoyl pentapeptide-3.

4. The multi-target efficacy composition according to any one of claims 1 to 3, characterized in that: The phospholipids in the oil-soluble collagen solution and the oil-soluble polypeptide solution each independently include lecithin; Preferably, the oil phases in the oil-soluble collagen solution and the oil-soluble polypeptide solution independently include an oily antioxidant and an oily solvent; Preferably, the oily antioxidant comprises tocopherol acetate; Preferably, the oily solvent includes any one of isopropyl myristate, caprylic / capric triglyceride, and isopropyl palmitate, or a combination of at least two thereof.

5. The multi-target efficacy composition according to any one of claims 1 to 4, characterized in that: The multi-target efficacy composition comprises, by weight, 0.1-2 parts of ascorbyl tetraisopalmitate, 0.1-3 parts of seabuckthorn extract, 0.1-1 part of phytol, 0.05-5 parts of oil-soluble collagen liquid and 0.05-5 parts of oil-soluble polypeptide liquid.

6. The multi-target efficacy composition according to any one of claims 1 to 5, characterized in that: The raw materials for preparing the oil-soluble collagen solution include, by weight percentage, 65-75% of oily solvent, 25-33% of phospholipid, 1-1.5% of water, 0.5-1% of oily antioxidant, 0.01-0.05% of soluble collagen, and 0.001-0.003% of pH regulator; Preferably, in terms of mass percentage, the raw materials for preparing the oil-soluble polypeptide liquid include 65-75% oily solvent, 25-33% phospholipids, 1-1.5% water, 0.5-1% oily antioxidant, and 0.001-0.1% polypeptide.

7. The multi-target efficacy composition according to any one of claims 1 to 6, characterized in that: The oil-soluble collagen solution is prepared by a preparation method comprising the following steps: (1) mixing the oil phase and the phospholipid to form a mixed solution I; (2) adding the aqueous phase dropwise into the mixed solution I and stirring to form an oil-soluble collagen solution having a reverse micelle structure; Preferably, the mixing temperature in step (1) is 50 to 80° C., and the mixing time is 0.5 to 4 h; Preferably, the stirring speed in step (2) is 100 to 1000 rpm, the stirring temperature is 20 to 40° C., and the stirring time is 0.5 to 3 h.

8. The multi-target efficacy composition according to any one of claims 1 to 7, characterized in that: The oil-soluble polypeptide liquid is prepared by a preparation method comprising the following steps: (a) mixing the oil phase and the phospholipid to form a mixed solution II; (b) adding the aqueous phase dropwise into the mixed solution II and stirring to form an oil-soluble polypeptide solution having a reverse micelle structure; Preferably, the mixing temperature in step (a) is 50 to 80° C., and the mixing time is 0.5 to 4 h; Preferably, the stirring speed in step (b) is 100 to 1000 rpm, the stirring temperature is 20 to 40° C., and the stirring time is 0.5 to 3 h.

9. A method for preparing the multi-target efficacy composition according to any one of claims 1 to 8, characterized in that: The preparation method comprises the following steps: Ascorbyl tetraisopalmitate, sea buckthorn extract, phytol, oil-soluble collagen liquid and oil-soluble polypeptide liquid are mixed at 20-40° C. to obtain a multi-target efficacy composition.

10. Use of the multi-target efficacy composition according to any one of claims 1 to 8 in cosmetics.

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