Compound preparation for protecting liver and removing mildew and preparation method thereof

Abstract

The invention relates to a compound preparation for protecting liver and removing mildew. The compound preparation is mainly prepared from the following raw materials by weight: 5-10 g of astragalus membranaceus, 5-10 g of medlar, 3-5 g of liquorice, 5-10 g of folium mori, 5-10 g of schisandra chinensis, 5-10 g of radix bupleuri, 5-10 g of poria cocos, 5-10 g of clove, 6-8 g of herba epimedii and 5-10 g of dandelion. According to the compound preparation, chitosan microspheres are prepared through a pH adjusting method, and chitosan can achieve the targeting effect on the liver and meanwhile achieve slow release of medicine. The combined use of the traditional Chinese medicine components in the preparation has obvious effects of protecting liver, removing mildew and improving immunity.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine preparations, and specifically relates to a compound preparation for protecting the liver and removing mold and a preparation method thereof. Background Art

[0002] Mycotoxins are common contaminants in the pig farming industry and are found in feed and feed ingredients. Mycotoxins can cause immunosuppression in pigs, leading to decreased immunity and increased likelihood of disease, especially severe damage to the liver. Long-term intake of feed containing mycotoxins can have a serious impact on the health of pigs and even lead to a decline in the overall health of the pig herd, affecting the benefits of farming.

[0003] The liver plays an important role in detoxification and excretion in pigs. The liver is responsible for breaking down and eliminating toxic substances in the body, including mycotoxins, drug toxins, and endotoxins. If liver function is impaired, the pig's detoxification ability will decrease, leading to toxin accumulation, further affecting the pig's health and production performance.

[0004] At present, although there are some mold removal agents on the market, the effects are not ideal and there are often safety hazards. Therefore, it is particularly important to develop an efficient, safe, and residue-free liver-protecting mold removal preparation. Chitosan, as a natural polymer material, has good biocompatibility and degradability, and has broad application prospects in the field of drug carriers.

[0005] Astragalus has the effects of replenishing qi and strengthening the exterior, promoting diuresis and reducing swelling. It can enhance immunity and protect the liver.

[0006] The main function of wolfberry is to nourish the liver and kidneys, improve sperm quality and eyesight. Regular consumption can improve the body's immunity and protect the liver.

[0007] Licorice has the function of harmonizing medicinal properties, relieving pain, and protecting the liver.

[0008] Mulberry leaves have the effects of clearing the liver, improving eyesight, and soothing the liver and regulating qi.

[0009] Schisandra chinensis has the effects of astringing, protecting the liver and reducing enzymes.

[0010] Bupleurum has the effect of soothing the liver and relieving depression, raising Yang Qi, and can regulate the body's Qi and make it rise upward and outward.

[0011] Poria cocos has the effects of promoting water expelling, invigorating the spleen and calming the mind. In feed, Poria cocos can regulate the water metabolism in animals, enhance the body's detoxification ability, and help excrete mycotoxins and their metabolites.

[0012] Cloves are rich in volatile oils and have antibacterial and anti-inflammatory effects. They can inhibit the growth and reproduction of molds, reduce the number of molds in feed, and thus reduce the production of mycotoxins.

[0013] Xianlingpi nourishes kidney yang and strengthens tendons and bones. It can improve the immunity and anti-stress ability of animals, so that animals can better resist and recover when facing the invasion of mycotoxins.

[0014] Dandelion has the effects of clearing away heat, detoxifying, reducing swelling and dispersing nodules. It can promote the detoxification function of the liver and help the liver in animals metabolize and remove mycotoxins.

[0015] Studies have shown that Chinese medicine has a strong inhibitory effect on mold, and its mechanism includes the following four aspects: 1. It produces antibacterial effects on mold through multiple components and multiple targets; 2. Its functions of clearing away heat and detoxifying, drying dampness and relieving itching also help inhibit the growth of mold; 3. It can enhance the anti-infection ability by regulating the human immune system, thereby indirectly inhibiting the growth of mold; 4. It works directly by inhibiting the growth of mold. These Chinese medicines include Atractylodes macrocephala, Poria cocos, Clove, Patchouli, Millettia reticulata, Kuncao, Black snake, Safflower, Epimedium, Dandelion, Shrimp whisker grass, Chrysanthemum, Artemisia capillaris, Artemisia annua and Artemisia argyi.

[0016] The mechanism of action of Chinese medicine in removing mold is multifaceted, including directly inhibiting mold growth, regulating the immune system, and enhancing the antibacterial effect through the characteristics of multiple components and multiple targets. These mechanisms work together to give Chinese medicine a unique advantage in treating mold infections.

[0017] The present invention provides a compound preparation for protecting liver and removing mold and a preparation method thereof. The preparation adopts a pH adjustment method to prepare chitosan microspheres, wherein chitosan can achieve a targeted effect on the liver and simultaneously achieve a sustained release of the drug. The Chinese medicinal ingredients in the preparation are used in combination to protect liver and remove mold and improve immunity, and the ultrasonic extraction method is used to greatly improve the extraction rate of Chinese medicinal materials. All components are green raw materials, safe and residue-free. The preparation method of the present invention is simple and easy to implement, suitable for industrial production, and has broad application prospects. Summary of the invention

[0018] The present invention aims to overcome the defects of the prior art and provide a compound preparation for protecting liver and removing mold. The compound preparation adopts a pH adjustment method to prepare chitosan microspheres, wherein chitosan can achieve a targeted effect on the liver and simultaneously achieve a sustained release of the drug. The combined use of the traditional Chinese medicine components in the preparation has a significant effect of protecting liver and removing mold and improving immunity.

[0019] The second object of the present invention is to provide a method for preparing the above-mentioned compound preparation for protecting the liver and removing mold.

[0020] The third purpose of the present invention is to provide the effect of the above-mentioned compound preparation for liver protection and mold removal on the liver of sows, that is, its application in the preparation of liver protection and mold removal drugs.

[0021] To achieve the above object, the present invention adopts the following technical solution: A compound preparation for protecting liver and removing mold, which is mainly made of the following raw materials in weight ratio: Astragalus 5-10g, wolfberry 5-10g, licorice 3-5g, mulberry leaf 5-10g, Schisandra 5-10g, Bupleurum 5-10g, Poria 5-10g, Clove 5-10g, Epimedium 6-8g, Taraxacum 5-10g.

[0022] As a preferred embodiment, the compound preparation for liver protection and mold removal is mainly made of the following raw materials in the following weight ratio: 5g of Astragalus, 10g of Lycium barbarum, 3g of Licorice, 10g of Mulberry Leaf, 5g of Schisandra, 10g of Bupleurum, 5g of Poria, 10g of Clove, 6g of Epimedium, and 10g of Taraxacum.

[0023] As a preferred embodiment, the compound preparation for liver protection and mold removal is mainly made of the following raw materials in the following weight ratio: 10g of Astragalus, 5g of Lycium barbarum, 5g of Licorice, 5g of Mulberry Leaf, 10g of Schisandra, 5g of Bupleurum, 10g of Poria, 5g of Clove, 8g of Epimedium, and 5g of Taraxacum.

[0024] As a preferred embodiment, the compound preparation for liver protection and mold removal is mainly made of the following raw materials in the following weight ratio: 7.5g of Astragalus, 7.5g of Lycium barbarum, 4g of Licorice, 7.5g of Mulberry Leaf, 7.5g of Schisandra, 7.5g of Bupleurum, 7.5g of Poria, 7.5g of Clove, 7g of Epimedium, and 7.5g of Taraxacum.

[0025] The present invention provides a method for preparing the above-mentioned compound preparation for protecting liver and removing mold, which comprises the following steps: (1) Preparation of chitosan solution: dissolving chitosan in 1-3% acetic acid solution (e.g., heating to 75-90° C. and maintaining for 15-30 minutes to promote dissolution) to obtain a chitosan solution; (2) Preparation of Chinese medicine extract: Wash, dry and crush (crush to 10-80 mesh) Astragalus, wolfberry, liquorice, mulberry leaf, Schisandra chinensis, Bupleurum, Poria, clove, Epimedium and dandelion, add 50-80% (volume percentage) ethanol solution, extract by ultrasonic, and concentrate to a solution with a relative density of 1.1-1.3 for later use; (3) The Chinese medicine extract obtained in step (2) is mixed with the chitosan solution obtained in step (1), and then Tween-80 is added to form an emulsion, and the pH value is adjusted to 7.4±0.2. After filtering, vacuum drying is performed to obtain the product.

[0026] Specifically, in step (1) of the above preparation method, the concentration of the chitosan solution can be 0.5-1.0% (mass percentage).

[0027] Specifically, in step (2), the ethanol solution can be added at a material-liquid ratio of 1 g: 20-30 mL. Furthermore, in step (2), the power of ultrasonic extraction is 400-600 W, the temperature is 40-75° C., and the time is 15-45 min.

[0028] Specifically, in step (3), the Chinese medicine extract and the chitosan solution can be mixed at a mass ratio of 1-3:1, and then 1.5-3.5 g of Tween-80 is added to form an emulsion.

[0029] The present invention provides a method for using the compound preparation for protecting liver and removing mold, which is specifically: mixed feeding, mixing 50kg of feed for every 80-120g of the compound preparation, which can be used for a long time.

[0030] The present invention provides an application of the above compound preparation in preparing a liver-protecting and mold-free drug for pigs (especially sows).

[0031] Compared with the prior art, the present invention has the following advantages and beneficial effects: (1) The preparation of the present invention adopts a reasonable combination of Chinese medicines, which can significantly improve liver function, enhance liver detoxification and improve the immunity of animals; (2) The preparation of the present invention has the function of removing mold and purifying toxins in the body, and can fundamentally improve the physical fitness of animals; after the compound preparation of the present invention is added to pig feed, it can effectively reduce the activity of enzymes such as alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in the serum, promote the reduction of tear spots in pigs, improve the shedding of dandruff, and make the hair color brighter; (3) The chitosan microspheres prepared by the pH adjustment method of the preparation of the present invention have uniform particle size distribution, good biocompatibility and degradability, pH responsiveness and good stability, and avoid the toxicity and environmental pollution caused by the use of traditional curing agents; (4) The present invention adopts ultrasonic extraction method, which can cause the plant cell wall to rupture or deform, thereby releasing the effective ingredients of traditional Chinese medicine more fully, and the extraction rate is increased by 50-500% compared with the traditional process; (5) The present invention adopts an oral preparation, which is convenient for clinical administration, suitable for group administration, and saves manpower and material resources. DETAILED DESCRIPTION

[0032] The technical solution of the present invention is further described in detail below in conjunction with the embodiments, but the protection scope of the present invention is not limited thereto.

[0033] In the following examples, all the raw materials used are common commercially available products that can be directly purchased.

[0034] Example 1. Preparation of a compound preparation for protecting liver and removing mold A compound preparation for protecting the liver and removing mold, which is made of the following raw materials in the following weight ratio: Astragalus 5g, wolfberry 10g, licorice 3g, mulberry leaf 10g, Schisandra 5g, Bupleurum 10g, Poria 5g, Clove 10g, Epimedium 6g, Taraxacum 10g.

[0035] The preparation method of the above-mentioned compound preparation for protecting liver and removing mold comprises the following steps: (1) Preparation of chitosan solution: chitosan was added to 1% acetic acid solution, heated to 85°C and maintained for 20 minutes to dissolve, thereby obtaining a chitosan solution.

[0036] (2) Preparation of Chinese medicine extract: Wash, dry and grind astragalus, wolfberry, liquorice, mulberry leaf, schisandra, bupleurum, poria, clove, epimedium and dandelion to 24 mesh size, add 70% (volume fraction) ethanol solution (solid-liquid ratio of 1 g:25 mL), perform ultrasonic extraction for 30 minutes, and concentrate to a solution with a relative density of 1.2 for later use.

[0037] (3) The Chinese medicine extract was added to the chitosan solution at a mass ratio of 1:1, and after stirring evenly, 1.5 g of Tween-80 was added to form an emulsion. The pH value of the solution was adjusted to 7.4±0.2 to allow chitosan to precipitate in the emulsion droplets. After filtering, the mixture was vacuum dried at 60°C to obtain the product of the present invention.

[0038] In this method: (1) the concentration of the chitosan solution in step 1 is 0.5%; (2) the power of ultrasonic extraction in step 2 is 500 W and the temperature is 60°C.

[0039] Example 2. Preparation of a compound preparation for protecting liver and removing mold A compound preparation for protecting the liver and removing mold, which is made of the following raw materials in the following weight ratio: Astragalus 10g, wolfberry 5g, licorice 5g, mulberry leaf 5g, Schisandra 10g, Bupleurum 5g, Poria 10g, Clove 5g, Epimedium 8g, Taraxacum 5g.

[0040] The preparation method of the above-mentioned compound preparation for protecting liver and removing mold comprises the following steps: (1) Preparation of chitosan solution: chitosan was added to 1% acetic acid solution, heated to 85°C and maintained for 20 minutes to dissolve, thereby obtaining a chitosan solution.

[0041] (2) Preparation of Chinese medicine extract: Wash, dry and grind astragalus, wolfberry, liquorice, mulberry leaf, schisandra, bupleurum, poria, clove, epimedium and dandelion to 24 mesh size, add 70% (volume fraction) ethanol solution (solid-liquid ratio of 1 g:25 mL), perform ultrasonic extraction for 30 minutes, and concentrate to a solution with a relative density of 1.2 for later use.

[0042] (3) The Chinese medicine extract was added to the chitosan solution at a mass ratio of 1:1, and after stirring evenly, 3.5 g of Tween-80 was added to form an emulsion. The pH value of the solution was adjusted to 7.4±0.2 to allow chitosan to precipitate in the emulsion droplets. After filtering, the mixture was vacuum dried at 60°C to obtain the product of the present invention.

[0043] In this method: (1) the concentration of the chitosan solution in step 1 is 1.0%; (2) the power of ultrasonic extraction in step 2 is 500 W and the temperature is 60°C.

[0044] Example 3. Preparation of a compound preparation for protecting liver and removing mold A compound preparation for protecting the liver and removing mold, which is made of the following raw materials in the following weight ratio: Astragalus 7.5g, wolfberry 7.5g, licorice 4g, mulberry leaf 7.5g, Schisandra 7.5g, Bupleurum 7.5g, Poria 7.5g, Clove 7.5g, Epimedium 7g, and Taraxacum 7.5g.

[0045] The preparation method of the above-mentioned compound preparation for protecting liver and removing mold comprises the following steps: (1) Preparation of chitosan solution: chitosan was added to 1% acetic acid solution, heated to 85°C and maintained for 20 minutes to dissolve, thereby obtaining a chitosan solution.

[0046] (2) Preparation of Chinese medicine extract: Wash, dry and grind astragalus, wolfberry, liquorice, mulberry leaf, schisandra, bupleurum, poria, clove, epimedium and dandelion to 24 mesh size, add 70% (volume fraction) ethanol solution (solid-liquid ratio of 1 g:25 mL), perform ultrasonic extraction for 30 minutes, and concentrate to a solution with a relative density of 1.2 for later use.

[0047] (3) The Chinese medicine extract was added to the chitosan solution at a mass ratio of 1:1, and after stirring evenly, 2 g of Tween-80 was added to form an emulsion. The pH value of the solution was adjusted to 7.4±0.2 to allow chitosan to precipitate in the emulsion droplets. After filtering, the mixture was vacuum dried at 60°C to obtain the product of the present invention.

[0048] In this method: (1) the concentration of the chitosan solution in step 1 is 0.8%; (2) the power of ultrasonic extraction in step 2 is 500 W and the temperature is 60°C.

[0049] Comparative Example 1. Preparation of a reference compound preparation for liver protection and mold removal A reference substance for a compound preparation for protecting liver and removing mold, which is prepared from the following raw materials in the following weight ratio: Astragalus 7.5g, wolfberry 7.5g, licorice 4g, mulberry leaf 7.5g, Schisandra 7.5g, Bupleurum 7.5g, and Taraxacum 7.5g.

[0050] The preparation method of the above-mentioned compound preparation reference substance for liver protection and mold removal comprises the following steps: (1) Preparation of chitosan solution: chitosan was added to 1% acetic acid solution, heated to 85°C and maintained for 20 minutes to dissolve, thereby obtaining a chitosan solution.

[0051] (2) Preparation of Chinese medicine extract: Wash, dry and grind astragalus, wolfberry, licorice, mulberry leaf, schisandra, bupleurum and dandelion to 24 mesh, add 70% (volume fraction) ethanol solution (solid-liquid ratio of 1 g:25 mL), perform ultrasonic extraction for 30 minutes, and concentrate to a solution with a relative density of 1.2 for later use.

[0052] (3) The Chinese medicine extract was added to the chitosan solution at a mass ratio of 1:1, and after stirring evenly, 2 g of Tween-80 was added to form an emulsion. The pH value of the solution was adjusted to 7.4±0.2 to allow chitosan to precipitate in the emulsion droplets. After filtering, the mixture was vacuum dried at 60°C to obtain the product of the present invention.

[0053] In this method: (1) the concentration of the chitosan solution in step 1 is 0.8%; (2) the power of ultrasonic extraction in step 2 is 500 W and the temperature is 60°C.

[0054] Note: Poria cocos, clove and Epimedium were not added in this comparative example.

[0055] Comparative Example 2. Preparation of a reference compound preparation for liver protection and mold removal A reference substance for a compound preparation for protecting liver and removing mold, which is prepared from the following raw materials in the following weight ratio: Astragalus 7.5g, wolfberry 7.5g, licorice 4g, mulberry leaf 7.5g, Schisandra 7.5g, Bupleurum 7.5g, Poria 7.5g, Clove 7.5g, Epimedium 7g, and Taraxacum 7.5g.

[0056] The preparation method of the above-mentioned compound preparation reference substance for liver protection and mold removal comprises the following steps: (1) Preparation of chitosan solution: chitosan was added to 1% acetic acid solution, heated to 85°C and maintained for 20 minutes to dissolve, thereby obtaining a chitosan solution.

[0057] (2) Preparation of Chinese medicine extract: Wash, dry and grind astragalus, wolfberry, liquorice, mulberry leaf, schisandra chinensis, bupleurum, poria, clove, epimedium and dandelion to 24 mesh, add 70% (volume fraction) ethanol solution (solid-liquid ratio of 1 g:25 mL), extract with ultrasound (power of 500 W, temperature of 60°C) for 30 minutes, concentrate to a solution with a relative density of 1.2, prepare into granules by conventional techniques in the art, and dry to obtain the granules.

[0058] Note: Microsphere process was not used in this comparative example.

[0059] Test Example: Effect of the preparation of the present invention on liver function of sows Proceed as follows: 1. Drugs and reagents Test groups 1, 2, and 3: the preparations prepared in Examples 1, 2, and 3 were added respectively; Comparative groups 1 and 2: the preparations prepared in Comparative Examples 1 and 2 were added respectively.

[0060] (II) Test methods (1) Experimental design: 100 pregnant sows with obvious tear spots, more dandruff, and rough fur were selected as experimental subjects and divided into 5 groups, with 20 sows in each group. Drugs were added to the experimental group and the control group respectively.

[0061] The three test groups and two control groups were mixed-fed, with 100g of the drug mixed with 50kg of feed for 14 consecutive days, and free feeding, epidemic prevention and management measures were consistent with daily routine.

[0062] Determination indicators: Compare the changes in clinical symptoms of sows before and after administration, and detect the activities of enzymes such as alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in serum.

[0063] (2) Results and analysis Table 1 Comparison of liver enzyme activities Table 2 Clinical symptom changes of sows The results in Table 1 and Table 2 show that in terms of enzyme activity, the decrease in ALP, AST, and ALT values ​​in test groups 1, 2, and 3 was significantly higher than that in control groups 1 and 2, and the clinical appearance of the sows after administration was also significantly better than that in control groups 1 and 2, with fewer tear spots, dandruff falling off, and brighter fur. These changes indicate that the product of the present invention can remove mycotoxin very well, and has a significant protective effect on the liver of sows, can improve liver function, and reduce liver damage.

Claims

1. A compound preparation for protecting liver and removing mold, characterized in that: It is mainly made of the following raw materials in weight ratio: Astragalus 5-10g, wolfberry 5-10g, licorice 3-5g, mulberry leaf 5-10g, Schisandra 5-10g, Bupleurum 5-10g, Poria 5-10g, Clove 5-10g, Epimedium 6-8g, Taraxacum 5-10g.

2. The compound preparation for protecting liver and removing mold according to claim 1, characterized in that: The invention is mainly prepared from the following raw materials in the following weight ratio: 5g of astragalus, 10g of wolfberry, 3g of liquorice, 10g of mulberry leaf, 5g of schisandra chinensis, 10g of bupleurum, 5g of poria, 10g of clove, 6g of epimedium and 10g of dandelion.

3. The compound preparation for protecting liver and removing mold according to claim 1, characterized in that: The invention is mainly prepared from the following raw materials in the following weight ratio: 10g of astragalus, 5g of wolfberry, 5g of liquorice, 5g of mulberry leaf, 10g of schisandra, 5g of bupleurum, 10g of poria, 5g of clove, 8g of epimedium and 5g of dandelion.

4. The compound preparation for protecting liver and removing mold according to claim 1, characterized in that: The invention is mainly prepared from the following raw materials in the following weight ratio: 7.5g of astragalus, 7.5g of wolfberry, 4g of liquorice, 7.5g of mulberry leaf, 7.5g of schisandra chinensis, 7.5g of bupleurum, 7.5g of poria, 7.5g of clove, 7g of epimedium, and 7.5g of dandelion.

5. The method for preparing the compound preparation for protecting liver and removing mold according to any one of claims 1 to 4, characterized in that: The steps include: (1) Preparation of chitosan solution: dissolving chitosan in 1-3% acetic acid solution to obtain a chitosan solution; (2) Preparation of Chinese medicine extract: Wash, dry and crush astragalus, wolfberry, liquorice, mulberry leaf, schisandra, bupleurum, poria, clove, epimedium and dandelion, add 50-80% ethanol solution, extract by ultrasonic, and concentrate to a solution with a relative density of 1.1-1.3 for later use; (3) The Chinese medicine extract obtained in step (2) is mixed with the chitosan solution obtained in step (1), and then Tween-80 is added to form an emulsion, and the pH value is adjusted to 7.4±0.

2. After filtering, vacuum drying is performed to obtain the product.

6. The method for preparing the compound preparation for protecting liver and removing mold as claimed in claim 5, characterized in that: In step (1), the concentration of the chitosan solution is 0.5-1.0%.

7. The method for preparing the compound preparation for liver protection and mold removal according to claim 5, characterized in that: In step (2), ethanol solution is added according to the material-liquid ratio of 1 g: 20-30 mL; the power of ultrasonic extraction is 400-600 W, the temperature is 40-75° C., and the time is 15-45 min.

8. The method for preparing the compound preparation for liver protection and mold removal according to claim 5, characterized in that: In step (3), the Chinese medicine extract and the chitosan solution are mixed at a mass ratio of 1-3:1, and then 1.5-3.5 g of Tween-80 is added to form an emulsion.

9. The method for using the compound preparation for protecting liver and removing mold according to any one of claims 1 to 4, characterized in that: For mixed feeding, mix 80-120g of compound preparation with 50kg of feed.

10. Use of the compound preparation according to any one of claims 1 to 4 in the preparation of a liver-protecting and mold-free drug for pigs.

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