Preparation method and application of naturally-fermented agricultural Chinese rose enzyme

Abstract

The invention relates to the technical field of enzyme processing, and particularly discloses a preparation method and application of a naturally fermented agricultural Chinese rose enzyme, and the agricultural Chinese rose enzyme is prepared by the following steps: compounding fresh Chinese rose flowers with water, adding brown sugar for fermentation treatment, and finally filtering to obtain fermentation liquor, namely the agricultural enzyme. The effective enzyme nutrient solution can be prepared through the method, the enzyme nutrient solution is applied to the planting industry, a good planting effect is achieved, the way of resource utilization of the residual rosa chinensis flowers is developed, and the agricultural rosa chinensis enzyme prepared through natural fermentation is prepared by taking fresh rosa chinensis flowers as main raw materials and is a typical environment-friendly enzyme. The preparation process is simple, raw materials are easily available, and cost is low. The Chinese rose enzyme produced by the method is amber, and the physicochemical indexes and microbiological indexes of the Chinese rose enzyme meet the standards. The Chinese rose enzyme produced by the method can enhance the resistance of ornamental plants and greatly improve the ornamental value when being used for cultivating the ornamental plants.

Description

Technical Field

[0001] The invention belongs to the technical field of enzyme processing, and in particular relates to a method for preparing a naturally fermented agricultural rose enzyme and an application thereof. Background Art

[0002] Rose belongs to the genus Rosa of the Rosaceae family. It is native to my country and is now cultivated all over the country. It is known as the queen of flowers in the world and has a thousand-year cultivation history. It is widely used in gardening, medicine, food, and spice industries. Enzymes are products with specific biological activity made from animals, plants, fungi, etc. through microbial fermentation. It is a mixed fermentation liquid containing rich nutrients such as sugars, organic acids, minerals, vitamins, phenols, terpenes, and some important enzymes and other biologically active substances. Used in plant cultivation, it will improve the immunity of plants and promote their good growth.

[0003] At present, the preparation method of rose enzyme mainly uses barley, grains, fruits and vegetables, edible fungi and roses as the main raw materials, and adopts mixed fermentation technology of multiple strains and multi-stage fermentation. Although this method has the advantage of thorough fermentation, it fails to retain the original rose ingredients to the maximum extent due to the addition of a large number of other products. In addition, the operation is relatively complicated, with certain technical requirements, and the production cost is high, which makes it difficult to promote.

[0004] Although there are reports on the production of rose enzymes, most of them are used in the food, medicine, and health care products industries, and there are no reports on its direct use in the planting industry.

[0005] To solve the above problems, the present invention provides a method for preparing enzymes by completely natural fermentation of roses. The fermentation of this method does not use any other fermentation bacteria and additives. The prepared rose enzyme can retain the original effective ingredients to the maximum extent, and the product quality is easy to control and the time consumption is short. The enzyme prepared by this method is used for plant cultivation and has obvious effect of promoting its growth.

[0006] Natural fermentation is a process of fermentation under natural environmental conditions (light, temperature, humidity, etc.) without adding any other microbial agents or external regulators. Natural fermentation is simple to operate, low in cost, easy to promote, and the fermentation product can retain the original product's nutritional components and biological activity to the maximum extent.

[0007] The rose enzyme developed in this study is a typical agricultural environmentally friendly enzyme. Because it is easy to obtain materials and does not add any other auxiliary fermentation bacteria, the product can be used for the cultivation of garden plants and crops. Experiments have shown that the use of rose enzyme cultivation can significantly enhance plant stress resistance, promote its good growth, and improve its variety quality. In particular, it can significantly increase the number of flowers and prolong the flowering period of garden plants, so that the ornamental value is significantly improved.

[0008] The total area of ​​rose cultivation has exceeded 100,000 hm2 2 , accounting for more than 50% of the national planting area, with an annual output of more than 1 million tons. The production and application of roses also brings some environmental problems. At present, there are many reports on the research on rose pruning to control the flowering period. The analysis and research on rose pruning shows that rose pruning can leave more than 30% of the flowers. And the residual flowers left by pruning of floribunda roses can reach more than 60% or even more.

[0009] At present, there is no effective treatment mechanism for the residual flowers of rose pruning. The general treatment method is to directly discard them as garden waste, which causes improper waste of resources and pollutes the environment to a certain extent, and fails to maximize their value.

[0010] The resource utilization of rose withered flowers is a problem to be solved urgently, and the present invention arises at the historic moment. Summary of the invention

[0011] The purpose of the present invention is to provide a method for preparing a naturally fermented agricultural rose enzyme and its application to solve the problems raised in the above-mentioned background technology.

[0012] To achieve the above object, the present invention provides the following technical solutions:

[0013] (1) Raw material pretreatment: Take fresh rose flowers, clean, wash, dry, and shred for later use. Water is taken from tap water, the hardness of the water is 8 to 12 degrees, and the pH value of the water is 6 to 8.

[0014] (2) Mixing: Mix water, pretreated rose flowers and brown sugar in a mass ratio of 10:3:1 to obtain a mixed material, and place the mixed material in a fermentation barrel and stir evenly to obtain a mixed fermentation material;

[0015] (3) Fermentation: Cover the container with a lid and ferment the mixed fermentation material: seal and ferment at a temperature of 20-30°C for 25-30 days; perform the following operations once in the morning and evening every day; open the lid, stir the mixed fermentation material after opening the lid, and then cover the container with a lid again for fermentation.

[0016] During step (3), the gas is exhausted every day, and the fermentation liquid is fully stirred until the fermentation liquid no longer produces bubbles or very few bubbles are generated, and then the fermentation is stopped;

[0017] The fermentation liquid obtained from the above fermentation was filtered through a 150-mesh filter to obtain the preliminary fermentation liquid, i.e., the agricultural rose enzyme stock solution.

[0018] The rose enzyme initially fermented in the step is allowed to stand, and is naturally sealed and stored under light-shielding conditions for 2 to 3 months to obtain a finished agricultural rose enzyme.

[0019] Specifically, the brown sugar is a solid product with a sugar content of 35-55% made from sugarcane as a raw material through juice extraction, clarification, boiling, and lime process.

[0020] Furthermore, the rose flowers in step (1) are rose flowers left after pruning, and the pretreatment method is: the flowers are cleaned and cleaned, dried and then shredded for later use.

[0021] Furthermore, the mass ratio of the water, the pretreated rose flowers and brown sugar is 10:3:1, and the total nutrients (N+P205+K20) are 3-5.5%.

[0022] Furthermore, the sugar content of the brown sugar is 45-55%.

[0023] The present application also relates to an application of an agricultural rose enzyme, wherein the agricultural rose enzyme is a rose enzyme prepared by the above method, and is applied in the planting industry, specifically using the agricultural rose enzyme as liquid fertilizer.

[0024] The present application also provides a method for preparing an organic liquid fertilizer. The raw material of the organic liquid fertilizer is the agricultural rose enzyme prepared by the above method. The preparation method is: diluting the agricultural enzyme in different proportions to obtain the organic liquid fertilizer.

[0025] Furthermore, the method for using the organic liquid fertilizer is: applying it with water or diluting the obtained organic liquid fertilizer by 800 to 1000 times and spraying it evenly on the leaves of crops as foliar fertilizer.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] 1. The present application develops a way to utilize the residual rose flowers. The residual rose flowers are mixed with brown sugar and water in a specific mass ratio for fermentation treatment, and agricultural enzymes are finally obtained after filtration. The preparation of the agricultural enzymes is simple and the production cost is low. Experiments have found that the agricultural enzymes have significant effects on improving the ornamental value, quality, and stress resistance of garden plants.

[0028] 2. The present application also discloses a method for using the agricultural enzyme, specifically, preparing the agricultural enzyme into liquid fertilizer and using it in the cultivation of ornamental plants, which can not only make the plants grow strong, but also improve their ornamental value. DETAILED DESCRIPTION

[0029] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Embodiment 1:

[0031] This embodiment is a method for preparing an agricultural rose enzyme:

[0032] The preparation method comprises the following steps:

[0033] (1) Raw material pretreatment: remove impurities from the pruned rose flowers, wash and dry them, and then shred them for later use;

[0034] (2) Mixing: clean river water or well water, pre-treated rose flowers and brown sugar are mixed evenly in a mass ratio of 8:2:1 to obtain a mixed material, which is placed in a fermentation barrel. The brown sugar has a sugar content of 35%;

[0035] Day 1 to 20: Perform the following operations once in the morning and evening every day: Open the lid, stir the mixed fermentation material after opening the lid, and then cover the lid to seal the fermentation. After 30 days: Filter the material in the fermentation barrel with a 120-mesh filter to obtain the agricultural rose enzyme stock solution.

[0036] The rose enzyme initially fermented in the above step is allowed to stand and naturally sealed and stored under light-shielding conditions for 2 to 3 months to obtain the finished agricultural rose enzyme.

[0037] Embodiment 2:

[0038] This embodiment is another method for preparing agricultural rose enzyme:

[0039] The preparation method comprises the following steps:

[0040] (1) Raw material pretreatment: Clean and dry the trimmed rose flowers for later use:

[0041] (2) Mixing: Evenly mix water, pretreated rose flowers and brown sugar in a mass ratio of 10:3:1 to obtain a mixed material, and place the mixed material in a fermentation barrel to obtain a mixed fermentation material, which is placed in a fermentation barrel: the brown sugar has a sugar content of 50%;

[0042] Day 1 to 20: Perform the following operations once in the morning and evening every day: open the lid, stir the mixed fermentation material after opening the lid, and exhaust the air. After the exhaust is completed, cover the lid and continue fermentation; after the 30th day of fermentation, use a 120-mesh sieve to filter the material in the fermentation barrel to initially obtain agricultural enzymes.

[0043] The rose enzyme initially fermented in the above steps is allowed to stand and naturally sealed and stored under light-shielding conditions for 2 to 3 months to obtain a finished agricultural rose enzyme.

[0044] Embodiment 3:

[0045] This embodiment is a method for preparing the third agricultural enzyme;

[0046] The preparation method comprises the following steps:

[0047] (1) Raw material pretreatment: the pruned rose flowers are cleaned and dried for later use;

[0048] (2) Mixing: water, pretreated mandarin orange fruit and sugarcane molasses are mixed evenly in a mass ratio of 12:5:1 to obtain a mixed material, and the mixed material is placed in a fermentation barrel to obtain a mixed fermentation material: the brown sugar has a sugar content of 60%;

[0049] (3) Day 1 to Day 20: Perform the following operations once in the morning and once in the evening every day: open the lid, stir the mixed fermentation material after opening the lid, and exhaust the air. After the exhaust is completed, cover the lid and continue fermentation. After the 30th day of fermentation, use a 120-mesh sieve to filter the material in the fermentation barrel to obtain the preliminary agricultural enzyme.

[0050] The preliminary rose enzyme fermented in the above steps is allowed to stand and naturally sealed and stored under light-shielding conditions for 2 to 3 months to obtain the finished agricultural rose enzyme.

[0051] The agricultural rose enzymes produced in the above-mentioned Examples 1 to 3 can be used in the planting industry, specifically, the agricultural enzymes are used to prepare organic liquid fertilizers.

[0052] In order to verify the practical value of the agricultural rose enzyme prepared in Examples 1 to 3 above, the applicant conducted the following tests:

[0053] A total of 7 fermentation treatments are set up, namely:

[0054] Group 1: the treatment method described in Example 1;

[0055] Group 2: the treatment method described in Example 2;

[0056] Group 3: the treatment method described in Example 3;

[0057] Group 4 (control group): the mixture was: rose: water = 3:10, other methods were the same as Group 2;

[0058] This test started on April 20, 2023, and the fermentation filtrate was tested on the 30th, 40th, 60th and 90th days of fermentation. The test indicators were determined according to the physical and chemical indicators of plant enzymes for planting in "Plant Enzymes" (QB / T 5323-1018), including general physical and chemical indicators and characteristic physical and chemical indicators.

[0059] General physical and chemical index testing:

[0060] The comparison of general physical and chemical indicators of the above groups is shown in Table 1.

[0061] Table 1. General physical and chemical indexes of each group of agricultural rose enzymes

[0062]

[0063]

[0064] Characteristic physical and chemical index detection:

[0065] The comparison of the characteristic physical and chemical indicators of the above groups is shown in Table 2

[0066] Table 2 Determination of characteristic physical and chemical indicators of each group of agricultural rose enzymes

[0067]

[0068] In combination with Table 1 and Table 2, the preparation methods described in Examples 1 to 3 all showed a relatively high organic matter content in the rose agricultural enzymes obtained during the fermentation period, indicating that under the fermentation process conditions, it is conducive to the accumulation of organic matter in the rose enzyme nutrient solution: the high organic matter content can be used as an organic liquid fertilizer to provide crops with more sufficient growth nutrients; in addition, its protease activity and polyphenol content are also relatively high, both of which are excellent. All physical and chemical properties of Examples 1 to 3 of the present application are excellent.

[0069] In summary, the indicators of the agricultural rose enzymes described in Examples 1 to 3 are all good, but the second group has the best comprehensive performance. The applicant further uses the agricultural enzymes described in Example 2 to prepare organic liquid fertilizer, and uses the agricultural enzymes prepared in Example 2 as an example to verify the effect of Example 4:

[0070] Embodiment 4:

[0071] This embodiment provides a method for preparing an organic liquid fertilizer, and the preparation method is:

[0072] The agricultural rose enzyme obtained by the preparation method of Example 2 is diluted 500 to 1000 times with water to obtain the organic liquid fertilizer.

[0073] Test 2

[0074] In order to illustrate the practical value of the liquid fertilizer described in Example 4, the liquid fertilizer obtained in Example 4 was applied:

[0075] From July to August 2023, a test on improving soil structure and soil fertility by using agricultural rose enzymes was carried out. The test included the following steps:

[0076] Step 1: Prepare the plastic potting soil with holes at the bottom of the F180, fermented agricultural rose enzyme and other materials;

[0077] Step 2: Put the sieved loess into F180 plastic pots to a depth of about 7 cm. The experiment has 4 treatments and 1 control. Each treatment has 3 replicates, for a total of 15 pots.

[0078] Step 3: The enzyme ratios involved in the experiment were pure water (control), treatment 1 (enzyme: water = 1:100), treatment 2 (enzyme: water = 1:200), treatment 3 (enzyme: water = 1:400), and treatment 4 (enzyme: water = 1:800);

[0079] Step 4: The specific treatment method is as follows: water once every 4 days starting from July 23rd until August 20th, a total of 8 times;

[0080] Step 5: Starting from July 23, soil organic matter and total nitrogen samples were taken every 7 days until August 20, for a total of 5 samples.

[0081] Step 6: Sampling and analysis results show that on August 20, the organic matter content of treatment No. 4 was the highest, reaching 20.12 g / kg. The total nitrogen analysis of treatment No. 4 was the highest, reaching 4.86 g / kg;

[0082] Conclusion: When agricultural rose enzyme is diluted 1:800 times, soil organic matter and total nitrogen increase the most, and soil fertility is the best.

[0083] From July to August 2023, conduct a comparative test on improving soil fertility by simultaneously applying agricultural rose enzymes and traditional fertilizers (monopotassium phosphate, solid granular compound fertilizer, solid organic fertilizer);

[0084] The test consists of the following steps:

[0085] Step 1: Select the rose variety "Fairyland", the quantity is 48 pots. The cultivation container is a 1-gallon black plastic pot, fermented rose enzyme and other materials;

[0086] Step 2: The comparative fertilizers used in the test are potassium dihydrogen phosphate (1500 times), solid granular compound fertilizer (3g / plant), and solid organic fertilizer (6g / plant);

[0087] Step 3: The enzyme ratios used in the test are respectively treatment 1 (enzyme: water = 1:200), treatment 2 (enzyme: water = 1:400), and treatment 3 (enzyme: water = 1:800);

[0088] Step 4: A randomized experimental design method was used. The experiment set up 3 controls (each fertilizer was applied without enzyme treatment), 9 treatments (3 treatments for each fertilizer), 5 pots in each treatment, and a total of 60 pots;

[0089] Step 4: Data determination method: randomly select 3 pots of roses for each treatment to measure plant height, crown width, stem thickness, longest flower branch, and number of flowers;

[0090] Step 5. Starting from October 8, 2021, the above indicators will be measured and recorded.

[0091] Step 6. Record the results. Treatment 3 has the most obvious effect on fertilization, with plant height up to 54 cm, crown width up to 47 cm, stem thickness up to 1.6 cm, longest flower branch up to 20 cm, and number of flowers up to 3.

[0092] Conclusion: When the rose enzyme is diluted 1:800 times, soil organic matter and total nitrogen increase the most, and the fertility is the best.

[0093] 3. From July to August 2023, a trial on the application of agricultural rose enzymes to promote rose cultivation will be carried out simultaneously;

[0094] The test consists of the following steps:

[0095] Step 1: Select the rose variety "Fairyland", 15 pots in total. The cultivation container should be a 180×155 red plastic pot, fermented rose enzyme and other materials;

[0096] Step 2: The experiment was set up with 4 treatments and 1 control, and 3 replicates for each treatment;

[0097] Step 3: The enzyme ratios involved in the experiment were pure water (control), treatment 1 (enzyme: water = 1:100), treatment 2 (enzyme: water = 1:200), treatment 3 (enzyme: water = 1:400), and treatment 4 (enzyme: water = 1:800);

[0098] Step 4: The specific treatment method is as follows: water once every 4 days starting from July 23rd until August 20th, a total of 8 times;

[0099] Step 5: Spray the enzyme 3 times during the test, with an interval of 10 days between each spray. Data record: the number of leaves on the longest branch, leaf length, leaf width, and number of flower buds of each rose pot;

[0100] Step 6. Record the results. Treatment 3 has the most obvious effect on fertilization, with leaf length up to 7.4 cm, leaf width up to 5.4 cm, leaf number up to 7, bud number up to 20 cm, and flowering number up to 4.

[0101] Conclusion: The rose enzyme combined with other fertilizers can improve soil fertility to varying degrees, promote rose growth, and enhance the ornamental value of roses. The optimal concentration is 800 times.

[0102] It will be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will be a routine task of design, fabrication, and production for those of ordinary skill having the benefit of this disclosure without undue experimentation.

[0103] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A method for preparing a naturally fermented agricultural rose enzyme, characterized in that: The following steps are involved: 1) Raw material pretreatment: Take fresh rose flowers, clean them, dry them, and shred them for later use; 2) Mixing: Evenly mix water, pretreated rose flowers and brown sugar in a mass ratio of 10:3:1 and place in a fermentation barrel to obtain a mixed fermentation material. 3) Fermentation: Cover the lid and ferment the mixed fermentation material; the fermentation temperature is 20-30°C, and the fermentation is 20-30 days. During the fermentation period, perform the following operations every other day: open the lid to vent, stir the mixed fermentation material, and then cover the lid again to ferment; after 30 days of fermentation, filter the material in the fermentation barrel with a 120-mesh sieve to obtain the agricultural rose enzyme stock solution, and store it naturally in a sealed state under light-shielding conditions for 2-3 months to obtain the finished agricultural rose enzyme.

2. The method for preparing a naturally fermented agricultural rose enzyme according to claim 1, characterized in that: The rose flowers in step 1) are fresh rose flowers that are trimmed and picked, and the pretreatment method is: the flowers are washed and shredded for later use.

3. The method for preparing a naturally fermented agricultural rose enzyme according to claim 1, characterized in that: The mass ratio of the water, the pretreated rose flowers and the brown sugar is 10:3:

1.

4. The method for preparing a naturally fermented agricultural rose enzyme according to claim 1, characterized in that: The water in step 2) is clean river water or well water, the hardness of the water is 8-12, and the pH value is 6-8.

5. The method for preparing a naturally fermented agricultural rose enzyme according to claim 1, characterized in that: The sugar content of the brown sugar in step 2) is 45-55%.

6. Use of the naturally fermented agricultural rose enzyme according to any of claims 1-5 as liquid fertilizer in the planting industry.

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