Solid sample full-automatic DNA purification and amplification device

Abstract

The invention discloses a solid sample full-automatic DNA (deoxyribonucleic acid) purification and amplification device which comprises a workbench, and a sample sleeve plate, a centrifugal module, a film sealing module, a puncture module, a heating oscillation module, a plate moving module and a mechanical arm which are arranged on the workbench, the receiving plate is arranged below the sample plate, the side edges of the receiving plate and the sample plate are clamped into a whole, a sample hole is formed in the sample plate, a receiving hole is formed in the receiving plate, the sample hole is sleeved with the receiving hole, and the side walls, close to the liquid outlet, of the sample hole and the receiving hole are in interference fit. The centrifugal module is used for separating the sample plate from the receiving plate under the centrifugal effect after cracking; the film sealing module is used for attaching a glue sealing film on a sample hole of the sample sleeve plate; and the puncture module is used for puncturing the glue sealing film on the sample sleeve plate. The centrifugal module is adopted to automatically separate the sample plate and the receiving plate of the sample cleading, the sample cleading does not leak liquid in a coating heating state, and it is ensured that a cracking system is stable, oscillation and uniform mixing are thorough, and a detected material is fully soaked.

Description

Technical Field

[0001] The invention belongs to the technical field of biological equipment, and in particular relates to a fully automated DNA purification and amplification device for solid samples. Background Art

[0002] Solid samples from judicial, clinical, animal inspection, disease control and other laboratories (such as stool swabs, exfoliated cell collectors, environmental sampling swabs and other solid samples and tissues) need to be extracted and purified for macromolecular target substances such as proteins and nucleic acids. Sample lysis and release must be performed to collect the necessary sample cell and tissue lysis contents, and then centrifugation can be used to maximize the collection of sample extracts for subsequent DNA purification and reaction system construction.

[0003] At present, the mainstream automated processing technology for lysis and product recovery of solid samples is to directly absorb the lysis products from large containers. Due to the large amount of residual leachate, some solid carriers themselves absorb a large amount of liquid, which is not suitable for the processing of trace samples. In order to thoroughly obtain all the leachate, centrifugation is required, and currently most operations are performed by manual centrifugal sleeves. Since samples such as shed cells and tissues of forensic DNA samples are basically dry samples, the lysis process requires high-temperature incubation. In order to prevent liquid evaporation and contamination, it is necessary to seal the centrifugal filter tube containing the sample and embed it into a centrifuge tube with a larger diameter. After lysis and centrifugation, the centrifugal filter tube containing the sample carrier and the outer tube containing the centrifugal recovery product need to be separated before the lysis product can be taken out.

[0004] Currently, there is no automatic film sealing device. Due to the characteristics of the sample container being through-through, it does not support top sealing, bottom sealing, or centrifugal liquid recovery. Since the sample plate cannot be sealed during the lysis process (because sealing will cause expansion and leakage), an unsealed lysis sample plate will easily cause the liquid to evaporate during the heating process, which will not only have a poor sample lysis effect, but will also cause corrosion or contamination to the inside of the equipment, and does not meet the laboratory's standard requirements for sample handling (ENFSI: FSR-G-208:6.2.7).

[0005] Therefore, a fully automated DNA purification and amplification device for solid samples is urgently needed. Summary of the invention

[0006] In order to solve the defects of the prior art, the present invention provides a fully automated DNA purification and amplification device for solid samples.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions: The present invention provides a fully automated solid sample DNA purification and amplification device, comprising: A workbench, wherein the workbench is provided with a purification plate position and a trace reagent position; and arranged on the workbench: The sample sleeve plate comprises a sample plate and a receiving plate, wherein the receiving plate is arranged below the sample plate and the two sides are clamped together as one, the sample plate is provided with a sample hole, the receiving plate is provided with a receiving hole, the sample hole is sleeved in the receiving hole and the side walls of the two near the liquid outlet are interference fit; A centrifugal module, used for separating the sample plate and the receiving plate by centrifugation after lysis; A sealing film module, used for attaching a sealing film to the sample wells of the sample plate; A puncture module, used to puncture the sealing film on the sample set; A heating and shaking module is used to heat and shake the sample plate to achieve a lysis reaction; The plate moving module is used to move the sample plate according to the settings; The robotic arm is used to drive the plate moving module and the puncture module according to the settings.

[0008] Preferably, at least one set of two symmetrical side edges of the sample plate and the receiving plate are integrally connected by a mortise and tenon structure.

[0009] Preferably, the side wall of the sample hole near the liquid outlet is provided with a first sample transition section, a sample filtering and screening section, a second sample transition section and a sample liquid outlet section in sequence from top to bottom, and the side wall of the receiving hole near the liquid outlet is provided with a first receiving transition section, a receiving filtering and screening section, a second receiving transition section and a receiving liquid outlet section in sequence from top to bottom, and the first receiving transition section, the receiving filtering and screening section, the second receiving transition section and the receiving liquid outlet section are arranged one by one on the outside of the first sample transition section, the sample filtering and screening section, the second sample transition section and the sample liquid outlet section, and the sample liquid outlet section and the receiving liquid outlet section have an interference fit.

[0010] The centrifugal module comprises: A cabin body, the cabin body is a cylindrical structure, and a movable cabin door is provided on the top cover of the cabin body; A hanging basket, wherein the hanging basket comprises a first hanging basket body and a second hanging basket body, wherein the first hanging basket body and the second hanging basket body are arranged on a horizontal rotor, and a sample set plate positioner and a matching plate positioner are arranged on the first hanging basket body and the second hanging basket body, and are used to place the sample set plate and the matching plate respectively; A horizontal rotor, which is rotatably disposed on the bottom plate of the cabin and is used to drive the hanging basket to rotate around its center; A rotary drive motor, wherein the output shaft of the rotary drive motor is drivingly connected to the horizontal rotor, and is used to drive the horizontal rotor to rotate around its center; A door drive motor, the output shaft of which is drivingly connected to the door for opening or closing the door; The photoelectric sensor is arranged on the inner wall of the cabin and is used to sense that the first hanging basket body or the second hanging basket body has reached the inlet and outlet position of the cabin.

[0011] Preferably, it also includes a liquid processing module and a balancing plate, wherein the liquid processing module is used to inject liquid into the balancing plate, and the balancing plate is used to balance the centrifugal module.

[0012] Preferably, it further comprises a sample adding tip and a multi-channel pipetting module, wherein the sample adding tip is loaded by the multi-channel pipetting module and is used to transfer the lysate; and the multi-channel pipetting module is driven by a robotic arm.

[0013] Preferably, it also includes a DNA preservation plate and a PCR plate, and the plate moving module moves the DNA preservation plate and the PCR plate to the sealing module according to the settings.

[0014] Preferably, it further comprises a waste channel, wherein the waste channel is used for loading waste.

[0015] Preferably, it also includes a cover opening module, and the cover opening module is used to open and close the test tube cover.

[0016] Preferably, it also includes a barcode reader, which is used to scan the barcodes on the sample set and the sample tip to record the consumables information.

[0017] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention uses a centrifugal module to automatically separate the sample plate and the receiving plate of the sample set. The sample set will not leak when it is coated and heated, ensuring that the lysis system is stable, the oscillation mixing is thorough, and the sample is fully soaked.

[0018] (2) The present invention seals the DNA storage plate and temporarily stores it at low temperature, thereby reducing the evaporation loss of trace reagents and ensuring the stability of the template system.

[0019] (3) During the construction process of the amplification system, the present invention keeps the system at a low temperature to ensure the stability of the reaction system. After the system is constructed, the system is directly sealed and stored to ensure the reliability of the reaction system.

[0020] (4) The functional modules of the robot arm in the present invention can be automatically replaced as needed. The various combined sample addition channels not only meet the rapid distribution of large volumes of liquid, but also ensure the accuracy of small volume liquid processing. The liquid processing accuracy is higher and the range is wider. It can also cooperate with the liquid processing module, plate moving module, puncture module, cover opening module, barcode reader, etc. to realize the operation of various functional modules. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of a fully automated solid sample DNA purification and amplification device of the present invention; Figure 2 This is a schematic diagram of the structure of the sample sleeve in the present invention when it is not separated; Figure 3 It is a structural schematic diagram of the mortise and tenon structure of the present invention; Figure 4 It is a schematic diagram of the state structure after the sample set plate is separated in the present invention; Figure 5 It is a schematic diagram of the structure of the sample hole and the receiving hole after the sample sleeve plate is separated in the present invention; Figure 6 It is a schematic diagram of the structure of the sample hole and the receiving hole when the sample sleeve plate is not separated in the present invention; Figure 7 is a schematic diagram of the structure of the sample hole in the present invention; Figure 8 It is a structural cross-sectional view of the sample hole and the receiving hole when the sample sleeve plate is not separated in the present invention; Fig. 9 It is a structural schematic diagram of the present invention; Fig.10 It is a structural schematic diagram of the centrifugal module in the present invention; Fig.11 It is a structural side view of the centrifugal module in the present invention. DETAILED DESCRIPTION

[0022] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0023] In the description of the present invention, it should be understood that the terms "left" and "right" etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings of the specification, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0024] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0025] like Figures 1 to 11As shown, this embodiment provides a fully automated solid sample DNA purification and amplification device, including a workbench, and arranged on the workbench: a sample plate, a centrifugal module 3-2, a sealing module 3-1, a puncture module 3-3, a heating and shaking module 3-8, a plate transfer module 3-7 and a mechanical arm 3-18. The workbench is provided with a purification plate position 3-4 and a micro reagent position 3-10.

[0026] like Figures 2 to 8 As shown, the sample set includes a sample plate 1-1 and a receiving plate 1-2, wherein the receiving plate 1-2 is arranged below the sample plate 1-1, and the front and rear sides of the sample plate 1-1 and the receiving plate 1-2 are respectively connected as a whole by two mortise and tenon structures 1-4, and the left and right sides of the sample plate 1-1 and the receiving plate 1-2 are respectively connected as a whole by two mortise and tenon structures 1-4. The mortise and tenon structure can ensure that the sample plate 1-1 and the receiving plate 1-2 are closely combined, and can also realize the automatic separation of the sample plate 1-1 and the receiving plate 1-2 under the action of centrifugal force without the aid of other tools.

[0027] like Figure 6 and Figure 7 As shown, the sample plate 1-1 is provided with 24 sample holes, the receiving plate 1-2 is provided with 24 receiving holes, and the sample hole 1-6 is sleeved in the receiving hole 1-7. The side wall of the sample hole 1-6 near the liquid outlet is provided with the first sample transition section, the sample filtering and screening section, the second sample transition section and the sample liquid outlet section 1-5 in sequence from top to bottom, and the side wall of the receiving hole 1-7 near the liquid outlet is provided with the first receiving transition section, the receiving filtering and screening section, the second receiving transition section and the receiving liquid outlet section in sequence from top to bottom. The first receiving transition section, the receiving filtering and screening section, the second receiving transition section and the receiving liquid outlet section are arranged one by one on the outside of the first sample transition section, the sample filtering and screening section, the second sample transition section and the sample liquid outlet section 1-5. The sample liquid outlet section 1-5 and the receiving liquid outlet section are interference fit, and the sample filtering and screening section is filled with a sieve plate 1-3. The diameters of the first sample transition section, the second sample transition section, the sample liquid outlet sections 1-5, the first receiving transition section, the second receiving transition section, and the receiving liquid outlet section are arranged to gradually decrease from top to bottom. The design of the transition section facilitates the fixation of the sieve plate, and the interference fit structure can achieve both sealing and rapid and automatic separation under the action of centrifugal force.

[0028] like Fig.10 and Fig.11As shown, the centrifugal module 3-2 includes a cabin 5-3, a hanging basket 5-1, a horizontal rotor 5-2, a rotation drive motor, a cabin door drive motor and a photoelectric sensor. The cabin 5-3 is a cylindrical structure, and a movable cabin door is provided on the top cover of the cabin 5-3. The hanging basket 5-1 includes a first hanging basket body and a second hanging basket body, and the first hanging basket body and the second hanging basket body are arranged on the horizontal rotor 5-2. The first hanging basket body and the second hanging basket body are provided with a sample plate positioner and a matching plate positioner, which are respectively used to place the sample plate and the matching plate. The horizontal rotor 5-2 is rotatably arranged on the bottom plate of the cabin, and is used to drive the hanging basket 5-1 to rotate around its center. The output shaft of the rotation drive motor is transmission-connected with the horizontal rotor 5-2, and is used to drive the horizontal rotor to rotate around its center. The output shaft of the cabin door drive motor is transmission-connected with the cabin door, and is used to open or close the cabin door. The photoelectric sensor is arranged on the inner wall of the cabin, and is used to sense the first hanging basket body or the second hanging basket body reaching the inlet and outlet position of the cabin. The centrifugal module 3-2 is used to separate the sample plate 1-1 and the receiving plate 1-2 under centrifugal action after lysis. The rotary drive motor can adopt any existing structure that can drive the horizontal rotor to rotate, and this embodiment does not make any specific restrictions. The door drive motor can adopt any existing structure that can open or close the door, and this embodiment does not make any specific restrictions.

[0029] When centrifugation is required, the rotary drive motor starts to rotate at a low speed. When the first hanging basket body turns to the set position, the photoelectric sensor receives the photoelectric signal and the rotary drive motor is locked. At this time, the door drive motor drives the door to open, and the sample set is placed on the first hanging basket body. When the sample set is successfully placed, the door drive motor drives the door to close, and the rotary drive motor rotates at a low speed again; when the second hanging basket body moves to the set position, the photoelectric sensor receives the photoelectric signal and the rotary drive motor is locked. At this time, the door drive motor drives the door to open again, and the matching plate is placed on the second hanging basket body. When the matching plate is successfully placed, the door drive motor drives the door to close, and the rotary drive motor starts, and runs according to the set centrifugation time and speed. When the centrifugation is completed, the door is opened, and the sample plate 1-1 and the receiving plate 1-2 are taken out respectively.

[0030] In this embodiment, it also includes a sealing module 3-1, a puncture module 3-3, a heating and shaking module 3-8, a robotic arm 3-18, a liquid handling module 3-6, a matching plate 3-14, a sample tip 3-13, a multi-channel pipetting module 3-9, a DNA preservation plate 3-12, a PCR plate 3-11, a waste channel 3-17, an opening module 3-16 and a barcode reader 3-15. The sealing module 3-1 is used to attach a glue sealing film to the sample hole of the sample plate. The puncture module 3-3 is used to pierce the glue sealing film on the sample plate. The heating and shaking module 3-8 is used to heat and shake the sample plate to achieve a cleavage reaction. The plate moving module 3-7 is used to move the sample plate according to the setting. The robotic arm 3-18 is used to drive the plate moving module 3-7 and the puncture module 3-3 according to the setting. The liquid handling module 3-6 is used to inject liquid into the matching plate 3-14, and the matching plate 3-14 is used to balance the centrifugal module 3-2. The sample tip 3-13 is loaded by the multi-channel pipetting module 3-9 and is used to transfer the lysate; the multi-channel pipetting module 3-9 is driven by the robotic arm 3-18. The plate moving module 3-7 moves the DNA preservation plate 3-12 and the PCR plate 3-11 to the sealing module 3-1 according to the settings. The waste channel 3-17 is used to load waste. The lid opening module 3-16 is used to open and close the test tube lid. The barcode reader 3-15 is used to scan the barcodes on the sample set plate and the sample tip 3-13 to record the consumables information.

[0031] In this embodiment, the cover opening module can realize automatic opening and closing of the reagent tube cover, and cooperate with the puncture module to repeatedly add liquid and re-coat the sample processing plate, thereby improving the processing capacity of complex samples.

[0032] In this embodiment, a PCR plate is added to homogenize the sample concentration according to the fluorescence quantitative results. The DNA content of low-concentration samples can be increased through pre-amplification technology, thereby improving the detection success rate of trace samples.

[0033] Comparative experimental data of the pyrolysis process with and without film sealing show that during the high-temperature pyrolysis process, the amount of liquid evaporated in the open sample plate is 3.2 times that of the liquid evaporated in the sealed sample plate. The evaporated liquid not only leads to instability of the pyrolysis system and poor sample pyrolysis effect, but also accumulates in the working chamber for a long time after crystallization, increasing the risk of aerosol contamination and corrosion of equipment parts inside the working chamber.

[0034] In addition, test data on the storage of DNA templates with and without sealing at room temperature indicate that unsealed DNA templates will evaporate 1-2 microliters per hour in a laboratory environment. This is very disadvantageous for trace samples where the DNA solution obtained is already relatively small. The extracted template DNA should be sealed and temporarily stored as soon as possible to avoid unnecessary volatilization losses, which is an effective measure to ensure the test results.

[0035] The working principle of this embodiment is further described below: Conventional magnetic / silica bead extraction reagents and consumables are pre-packaged in a 48-well plate (injection molded product) that matches the purifier (8 servings / plate). The pre-filled reagents include lysis buffer, binding buffer, washing buffer, elution buffer, magnetic / silica beads, etc. Fig. 9 As shown, before the magnetic bead extraction work begins, the magnetic rod cover 2-2 for transferring magnetic beads can be placed in the plate hole of the reagent plate 2-1. The plate hole can keep the magnetic rod cover upright, which is convenient for loading the magnetic rod cover.

[0036] Preparation: The operator places the sample to be extracted in the sample set plate, the sample set plate is placed on the heating and shaking module 3-8, the pre-packed reagent plate 2-1 and the magnetic rod set 2-2 are placed on the purification plate position 3-4, and the sample tip 3-13 and the trace reagent are placed in the designated positions.

[0037] The following operations are all automated: the robot arm 3-18 automatically loads the plate transfer module 3-7, grabs the sample plate from the heating and oscillating module 3-8, and the sample tip 3-13 is sent to the barcode reader 3-15 to scan the barcode, and the consumables barcode information is recorded and confirmed.

[0038] The robot arm 3-18 automatically loads the liquid handling module 3-6, loads the sample tip 3-13, takes the lysate from the pre-packed purification plate position 3-4 and the protease PK from the trace reagent and adds them to the sample set plate respectively.

[0039] The robot arm 3-18 automatically loads the plate transfer module 3-7, transfers the sample plate to the sealing module 3-17, automatically seals the film, and then transfers the sealed sample plate to the heating and shaking module 3-8 to start heating and lysis, shaking several times during the process to fully break the sample cells.

[0040] After the lysis is completed, the plate transfer module 3-7 transfers the sample plate to the centrifugal module 3-2, and uses the liquid handling module 3-6 to inject liquid into the preparation plate 3-14 for balancing according to the number of samples. The preparation plate is transferred to the centrifugal module 3-2 and centrifugation is started.

[0041] Under the action of centrifugal force, the mortise and tenon structure 1-4 of the receiving plate 1-2 at the bottom of the sample sleeve and the sample plate 1-1 at the top will automatically separate, the interference fit at the bottom will naturally disappear, and the lysis product in the sample plate 1-1 will flow into the receiving plate under the action of centrifugal force.

[0042] After the centrifugation is completed, the centrifugal module opens the hatch, and the plate moving module 3-7 first takes out the sample plate, then takes out the receiving plate, and puts it back on the heating and shaking module 3-8 which has stopped heating.

[0043] The robot arm 3-18 automatically loads the multi-channel pipetting module 3-9, loads the sample tip 3-13, transfers the sample lysate recovered by centrifugation to the binding reagent well of the purification plate position 3-4, and discards the disposable pipette tip in the waste channel 3-21.

[0044] The magnetic rod 3-5 of the purification module is loaded with the magnetic rod sleeve 2-2, which adsorbs and transfers the magnetic beads to the binding wells for rotation and mixing, and the sample DNA will bind to the magnetic beads. The magnetic rod 3-5 is inserted into the magnetic rod sleeve 2-2, and after absorbing the magnetic beads, it is washed in the washing solution 1, 2, and 3 wells in turn. After washing three times, the magnetic rod sleeve 2-2 transfers the magnetic beads to the eluent wells. At this time, the eluent at the bottom of the elution wells is heated, and the eluent is heated to elute the DNA. After the DNA is eluted, the magnetic rod sleeve 2-2 adsorbs and transfers the magnetic beads to the lysate wells that have been emptied in the first column. The magnetic beads that complete the extraction task will remain in the first column of wells. The purifier tray carrier is automatically removed to complete the DNA extraction and purification steps.

[0045] The multi-channel pipetting module 3-9 is loaded with a sample tip 3-13, and the DNA eluate is transferred from the purification plate to the DNA preservation plate 3-12. After the transfer, the sample tip is discarded into the waste channel 3-21.

[0046] The liquid handling module 3-6 absorbs the prepared amplification reagent at the micro reagent position 3-10, distributes it to the PCR plate 3-11 constructed in the reaction system, and absorbs a certain amount of template from the DNA storage plate 3-12 and adds it to the reaction system solution of the PCR plate 3-11.

[0047] The plate transfer module 3-7 grabs the DNA preservation plate 3-12 and the PCR plate 3-11 respectively for sealing and preservation.

[0048] After the equipment completes the above work, it outputs the experimental report and the sample information location file. When other samples are directly sealed and lysed using a recovery plate, the puncture module 3-3 can pierce the puncturable plastic sealing film, and then the sample can be sucked up by the sample tip for processing. Trace reagents can be automatically unscrewed by the cover opening module 3-16, and the cover can be automatically screwed on after the liquid is sucked up to ensure the stability of the reagents. The functional modules can be automatically replaced and used according to the needs of the method flow.

[0049] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A fully automated DNA purification and amplification device for solid samples, characterized in that: include: A workbench, wherein the workbench is provided with purification plate positions (3-4) and trace reagent positions (3-10); And set on the workbench: A sample sleeve plate comprises a sample plate (1-1) and a receiving plate (1-2), wherein the receiving plate (1-2) is arranged below the sample plate (1-1) and the sides of the two are snap-fitted together, the sample plate (1-1) is provided with a sample hole (1-6), the receiving plate (1-2) is provided with a receiving hole (1-7), the sample hole (1-6) is sleeved in the receiving hole (1-7), and the side walls of the two near the liquid outlet are interference fit; A centrifugal module (3-2) is used to separate the sample plate (1-1) and the receiving plate (1-2) by centrifugation after lysis; A sealing film module (3-1), used for attaching a sealing film to the sample wells of the sample plate; The puncture module (3-3) is used to puncture the sealing film on the sample sleeve; A heating and shaking module (3-8) is used to heat and shake the sample plate to achieve a lysis reaction; The plate moving module (3-7) is used to move the sample plate according to the setting; The mechanical arm (3-18) is used to drive the plate moving module (3-7) and the puncture module (3-3) according to settings.

2. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: At least one set of two symmetrical side edges of the sample plate (1-1) and the receiving plate (1-2) are connected as one through a mortise and tenon structure (1-4).

3. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: The side wall of the sample hole (1-6) near the liquid outlet is provided with a first sample transition section, a sample filtering and screening section, a second sample transition section and a sample liquid outlet section (1-5) in sequence from top to bottom; the side wall of the receiving hole (1-7) near the liquid outlet is provided with a first receiving transition section, a receiving filtering and screening section, a second receiving transition section and a receiving liquid outlet section in sequence from top to bottom; the first receiving transition section, the receiving filtering and screening section, the second receiving transition section and the receiving liquid outlet section are arranged one by one on the outside of the first sample transition section, the sample filtering and screening section, the second sample transition section and the sample liquid outlet section (1-5); the sample liquid outlet section (1-5) and the receiving liquid outlet section are interference fit; and the sample filtering and screening section is filled with a sieve plate (1-3).

4. The fully automated DNA purification and amplification device for solid samples according to claim 1, characterized in that: The centrifugal module (3-2) comprises: A cabin (5-3), the cabin (5-3) being a cylindrical structure, and a movable cabin door being provided on the top cover of the cabin (5-3); A hanging basket (5-1), the hanging basket (5-1) comprising a first hanging basket body and a second hanging basket body, the first hanging basket body and the second hanging basket body being arranged on a horizontal rotor (5-2), the first hanging basket body and the second hanging basket body being provided with a sample plate positioner and a matching plate positioner, which are used to place the sample plate and the matching plate respectively; A horizontal rotor (5-2), the horizontal rotor (5-2) being rotatably disposed on the bottom plate of the cabin body and being used to drive the hanging basket (5-1) to rotate around its center; A rotary drive motor, the output shaft of which is in driving connection with the horizontal rotor (5-2) and is used to drive the horizontal rotor to rotate around its center; A door drive motor, the output shaft of which is drivingly connected to the door for opening or closing the door; The photoelectric sensor is arranged on the inner wall of the cabin and is used to sense that the first hanging basket body or the second hanging basket body has reached the inlet and outlet position of the cabin.

5. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also comprises a liquid processing module (3-6) and a matching plate (3-14), wherein the liquid processing module (3-6) is used to inject liquid into the matching plate (3-14), and the matching plate (3-14) is used to balance the centrifugal module (3-2).

6. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also includes a sample adding tip (3-13) and a multi-channel liquid transfer module (3-9), wherein the sample adding tip (3-13) is loaded by the multi-channel liquid transfer module (3-9) and is used to transfer the lysate; and the multi-channel liquid transfer module (3-9) is driven by a mechanical arm (3-18).

7. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also includes a DNA preservation plate (3-12) and a PCR plate (3-11), and the plate moving module (3-7) moves the DNA preservation plate (3-12) and the PCR plate (3-11) onto the sealing module (3-1) according to settings.

8. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also includes a waste channel (3-17), wherein the waste channel (3-17) is used to load waste.

9. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also comprises a cover opening module (3-16), wherein the cover opening module (3-16) is used for opening and closing the test tube cover.

10. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also includes a barcode reader (3-15), which is used to scan the barcodes on the sample set plate and the sample tip (3-13) to record the consumables information.

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