Integrated cell culture device

Abstract

The invention discloses an integrated cell culture device which comprises a cavity, a guide inner cavity, an environment module, a cell culture module, a sealing assembly and an observation assembly, the top of the cavity is connected with a plurality of transom windows; the plurality of guide inner cavities are connected to the interior of the cavity; the environment module is connected to one side of the exterior of the cavity; the cell culture module is connected to the other side of the exterior of the cavity; the sealing assembly is connected to the outer side of the cavity; the observation assembly is connected to the top of the cavity. A culture space is provided through the cavity, the environment module is placed through the guide inner cavity, the environment module can be pre-adjusted externally, when culture needs to be carried out, the environment module can rapidly enter a culture state after being directly installed, the environment module can provide adjustment of factors such as gas, temperature and pressure, and all the modules do not interfere with one another and are replaced after being disassembled; the environment can be pre-adjusted, the device can be quickly used, and when the next group of culture is carried out, the culture can be directly continued after being taken out.

Description

Technical Field

[0001] The invention belongs to the technical field of integrated cell culture, and in particular relates to an integrated cell culture device. Background Art

[0002] A cell culture apparatus is a device used to grow and maintain cells in the laboratory.

[0003] Integrated cell culture devices are highly integrated systems that integrate multiple functional modules to simplify the cell culture process and improve efficiency and reliability. These devices usually include functions such as temperature control and gas control, and are suitable for various cell culture needs.

[0004] When conducting cell culture, different environmental parameters need to be changed due to different cell types. However, it is very troublesome to adjust the parameters of current culture instruments. When culturing different cells, the environment needs to be temporarily changed. This process is very troublesome. Therefore, there is an urgent need for an integrated cell culture device that can quickly and modularly adjust the culture environment. Summary of the invention

[0005] Based on the above technical problems, the present invention provides an integrated cell culture device.

[0006] The specific technical solution is: An integrated cell culture device, comprising: A cavity, wherein a plurality of top windows are connected to the top of the cavity; A guide inner cavity, wherein a plurality of the guide inner cavities are connected to the interior of the cavity; An environmental module, the environmental module is connected to one side outside the cavity; A cell culture module, wherein the cell culture module is connected to the other side of the cavity; A sealing component connected to the outside of the cavity; An observation component is connected to the top of the cavity.

[0007] In the above technical solution, the environment module includes: A box body, wherein the top of the box body is connected with an observation window; An end cover connected to one side of the box body; A plurality of mobile motors are connected to one side of the box body, and a threaded rod is connected to the driving end of the mobile motor; A mobile rack, wherein the plurality of threaded rods are all screwed to the bottom of the mobile rack, one end of the threaded rod is movably connected to one side of the inner part of the box body, a wiping plate is connected to the top of the mobile rack, and a wiping cloth is arranged on the top of the wiping plate; An ultraviolet germicidal lamp, the ultraviolet germicidal lamp is connected to one side of the interior of the box body; A pressurizing component is connected to one side of the box body.

[0008] In the above technical solution, the pressurizing component includes: A balancing port is opened on one side of the box body.

[0009] The above technical solution also includes: An inflatable ring tube, the inflatable ring tube is connected to the box body; A humidifying ring tube is connected to one side of the box body.

[0010] In the above technical solution, the cell culture module includes: A sliding base, wherein the sliding base slides on one side of the cavity; A mounting plate, the mounting plate is connected to the top of the sliding base, and the top of the mounting plate is connected to a limiting block; A culture dish, the culture dish being placed on top of the mounting plate; A temperature control module is connected to the sliding base.

[0011] In the above technical solution, the sealing assembly includes: A first sealing seat, wherein the first sealing seat is connected to one side of the cavity; a second sealing seat, the second sealing seat being connected to the other side of the cavity; A first docking seat, wherein the first docking seat is connected to the bottom of the box body; A second docking seat is connected to one side of the sliding base.

[0012] In the above technical solution, the observation component includes: End guide rails, two pairs of end guide rails are respectively connected to two sides of the cavity; A slide, one side of which is connected to an end slide block, and the end slide block is slidably connected to a pair of end guide rails; A transverse moving motor, wherein the transverse moving motor is connected to one end of a slide, an intermediate gear is movably connected to one end of the slide, a driving end of the transverse moving motor is connected to the moving gear, and the moving gear is meshed with the intermediate gear; A rack, the rack being connected to one side of the cavity, and the moving gear being meshed with the rack; A microscope electron microscope, wherein a plurality of the microscope electron microscopes are connected to the middle of the slide.

[0013] In the above technical solution, the temperature control module includes: A temperature exchange pipe, wherein the temperature exchange pipe is connected to the sliding base; Thermally conductive silicone grease is filled in the sliding base.

[0014] The above technical solution also includes: A base is installed at the bottom of the cavity.

[0015] Compared with the prior art, the integrated cell culture device of the present invention has the following beneficial effects: The culture space is provided by the cavity, and the environmental module is placed in the guiding inner cavity. The environmental module can be pre-adjusted externally. When culture is needed, it can be directly installed to quickly enter the culture state. The environmental module can provide adjustment of factors such as gas, temperature and pressure. Each module does not interfere with each other and can be replaced at will. The environment can be pre-adjusted and used quickly. When the next group of culture is carried out, the culture can be continued directly after being taken out. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic structural diagram of an integrated cell culture device of the present invention; Figure 2 It is a schematic diagram of the present invention; Figure 3 It is a schematic diagram of the present invention; Figure 4 It is a schematic diagram of the present invention; Figure 5 It is a cross-sectional schematic diagram of the present invention; Figure 6 It is a schematic cross-sectional view of the present invention.

[0017] in, Figures 1 to 6 The corresponding relationship between the reference numerals and component names in the figure is: 1. Cavity; 2. Guide inner cavity; 3. Box body; 4. End cover; 5. Moving motor; 6. Moving frame; 7. Ultraviolet germicidal lamp; 8. Balance port; 9. Inflatable ring tube; 10. Humidification ring tube; 11. Sliding base; 12. Mounting plate; 13. Culture dish; 14. First sealing seat; 15. Second sealing seat; 16. First docking seat; 17. Second docking seat; 18. End guide slide rail; 19. Slide; 20. Lateral moving motor; 21. Rack; 22. Electron microscope; 23. Temperature exchange tube; 24. Base. DETAILED DESCRIPTION

[0018] The following is a combination of specific implementation cases and attached Figure 1-6 The present invention is further described below, but the present invention is not limited to these embodiments.

[0019] like Figure 1-6As shown, the present invention discloses an integrated cell culture device, comprising: a cavity 1, a guide cavity 2, an environment module, a cell culture module, a sealing component and an observation component; a plurality of top windows are connected to the top of the cavity 1; a plurality of guide cavities 2 are connected to the inside of the cavity 1; the environment module is connected to one side of the outside of the cavity 1; the cell culture module is connected to the other side of the outside of the cavity 1; the sealing component is connected to the outside of the cavity 1; and the observation component is connected to the top of the cavity 1.

[0020] In an embodiment of the present invention, Figure 1-6 As shown, the environmental module includes: a box body 3, an end cover 4, a mobile motor 5, a mobile frame 6, an ultraviolet sterilization lamp 7 and a pressurizing component; an observation window is connected to the top of the box body 3; the end cover 4 is connected to one side of the box body 3; a number of mobile motors 5 are connected to one side of the inside of the box body 3, and the driving end of the mobile motor 5 is connected to a threaded rod; a number of threaded rods are screwed to the bottom of the mobile frame 6, one end of the threaded rod is movably connected to one side of the inside of the box body 3, a wiping plate is connected to the top of the mobile frame 6, and a wiping cloth is arranged on the top of the wiping plate; the ultraviolet sterilization lamp 7 is connected to one side of the inside of the box body 3; and the pressurizing component is connected to one side of the box body 3.

[0021] In an embodiment of the present invention, Figure 1-6 As shown, the pressurizing component includes: a balancing port 8; the balancing port 8 is opened on one side of the box body 3.

[0022] In an embodiment of the present invention, Figure 1-6 As shown, it also includes: an air filling ring tube 9 and a humidifying ring tube 10; the air filling ring tube 9 is connected to the box body 3; and the humidifying ring tube 10 is connected to one side of the box body 3.

[0023] In an embodiment of the present invention, Figure 1-6 As shown, the cell culture module includes: a sliding base 11, a mounting plate 12, a culture dish 13 and a temperature control module; the sliding base 11 slides on one side of the cavity 1; the mounting plate 12 is connected to the top of the sliding base 11, and the top of the mounting plate 12 is connected to a limited position block; the culture dish 13 is placed on the top of the mounting plate 12; and the temperature control module is connected to the sliding base 11.

[0024] In an embodiment of the present invention, Figure 1-6 As shown, the sealing assembly includes: a first sealing seat 14, a second sealing seat 15, a first docking seat 16 and a second docking seat 17; the first sealing seat 14 is connected to one side of the cavity 1; the second sealing seat 15 is connected to the other side of the cavity 1; the first docking seat 16 is connected to the bottom of the box body 3; the second docking seat 17 is connected to one side of the sliding base 11.

[0025] In an embodiment of the present invention, Figure 1-6As shown, the observation assembly includes: an end guide rail 18, a slide 19, a lateral movement motor 20, a rack 21 and a microscope 22; two pairs of end guide rails 18 are respectively connected to the two sides of the cavity 1; an end slider is connected to one side of the slide 19, and the end slider is slidably connected to a pair of end guide rails 18; the lateral movement motor 20 is connected to one end of the slide 19, and an intermediate gear is movably connected to one end of the slide 19, and the driving end of the lateral movement motor 20 is connected to the moving gear; the moving gear is meshed with the intermediate gear; the rack 21 is connected to one side of the cavity 1, and the moving gear is meshed with the rack 21; a number of microscopes 22 are connected to the middle of the slide 19.

[0026] In an embodiment of the present invention, Figure 1-6 As shown, the temperature control module includes: a temperature exchange tube 23 and thermal conductive silicone grease; the temperature exchange tube 23 is connected to the sliding base 11; and the thermal conductive silicone grease is filled in the sliding base 11.

[0027] In an embodiment of the present invention, Figure 1-6 As shown, it also includes: a base 24; the base 24 is installed at the bottom of the cavity 1.

[0028] Implementation process: First, insert the box body 3 into the inner cavity, and then connect the first sealing seat 14 to the first docking seat 16 by bolts. After the connection is completed, insert the cell culture module into the inner cavity from the other side and enter into the box body 3, and connect the second sealing seat 15 and the second docking seat 17 by bolts. After the connection, except for the pressurization conditions that need to be adjusted on site, all other conditions can be adjusted before culture. Connect the humidifier to the humidification ring tube 10 to humidify the interior, and change the gas concentration in the box body 3 through the inflation ring tube 9 through the inflation device. When adjusting the gas and humidification, block the open side of the box body 3 so that the internal gas is discharged through the balance port 8. After adjusting to balance, install it. After the installation is completed, culture is carried out, and the slide 19 is moved to observe different cell culture modules. If the inside of the box body 3 is fogged, control the driving end of the mobile motor 5 to rotate so that the wiping cloth wipes the bottom of the observation window.

[0029] In the description of the present invention, the term "plurality" refers to two or more than two. Unless otherwise clearly defined, the orientation or positional relationship indicated by the terms "upper" and "lower" is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention. The terms "connection", "installation", "fixation", etc. should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] In the description of the present invention, the description of the terms "one embodiment", "some embodiments", "specific embodiments", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In the present invention, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0031] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An integrated cell culture device, characterized in that: include: A cavity, wherein a plurality of top windows are connected to the top of the cavity; A guide inner cavity, wherein a plurality of the guide inner cavities are connected to the interior of the cavity; An environmental module, the environmental module is connected to one side outside the cavity; A cell culture module, wherein the cell culture module is connected to the other side of the cavity; A sealing component connected to the outside of the cavity; An observation component is connected to the top of the cavity.

2. The integrated cell culture device according to claim 1, characterized in that: The environment module includes: A box body, wherein the top of the box body is connected with an observation window; An end cover connected to one side of the box body; A plurality of mobile motors are connected to one side of the box body, and a threaded rod is connected to the driving end of the mobile motor; A mobile rack, wherein the plurality of threaded rods are all screwed to the bottom of the mobile rack, one end of the threaded rod is movably connected to one side of the inner part of the box body, a wiping plate is connected to the top of the mobile rack, and a wiping cloth is arranged on the top of the wiping plate; An ultraviolet germicidal lamp, the ultraviolet germicidal lamp is connected to one side of the interior of the box body; A pressurizing component is connected to one side of the box body.

3. The integrated cell culture device according to claim 2, characterized in that: The pressurizing component comprises: A balancing port is opened on one side of the box body.

4. The integrated cell culture device according to claim 2, characterized in that: Also includes: An inflatable ring tube, the inflatable ring tube is connected to the box body; A humidifying ring tube is connected to one side of the box body.

5. The integrated cell culture device according to claim 4, characterized in that: The cell culture module comprises: A sliding base, wherein the sliding base slides on one side of the cavity; A mounting plate, the mounting plate is connected to the top of the sliding base, and the top of the mounting plate is connected to a limiting block; A culture dish, the culture dish being placed on top of the mounting plate; A temperature control module is connected to the sliding base.

6. The integrated cell culture device according to claim 1, characterized in that: The sealing assembly comprises: A first sealing seat, wherein the first sealing seat is connected to one side of the cavity; a second sealing seat, the second sealing seat being connected to the other side of the cavity; A first docking seat, wherein the first docking seat is connected to the bottom of the box body; A second docking seat is connected to one side of the sliding base.

7. The integrated cell culture device according to claim 1, characterized in that: The observation component comprises: End guide rails, two pairs of end guide rails are respectively connected to two sides of the cavity; A slide, one side of which is connected to an end slide block, and the end slide block is slidably connected to a pair of end guide rails; A transverse moving motor, wherein the transverse moving motor is connected to one end of a slide, an intermediate gear is movably connected to one end of the slide, a driving end of the transverse moving motor is connected to the moving gear, and the moving gear is meshed with the intermediate gear; A rack, the rack being connected to one side of the cavity, and the moving gear being meshed with the rack; A microscope electron microscope, wherein a plurality of the microscope electron microscopes are connected to the middle of the slide.

8. The integrated cell culture device according to claim 5, characterized in that: The temperature control module comprises: A temperature exchange pipe, wherein the temperature exchange pipe is connected to the sliding base; Thermally conductive silicone grease is filled in the sliding base.

9. The integrated cell culture device according to claim 1, characterized in that: Also includes: A base is installed at the bottom of the cavity.

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