Metarhizium anisopliae algal glue trapping and killing granule for controlling wireworms in bamboo forest and preparation method

Abstract

The invention discloses metarhizium anisopliae algal gum trapping and killing granules for controlling wireworms in a bamboo forest and a preparation method. Each 100ml of water contains the following components by weight: 1-3g of sodium alginate, 0.7-0.8 g of metarhizium anisopliae spore powder, 1-3g of saccharomycetes, 7-9g of attapulgite and 0.3-0.7 g of rice meal. The green muscardine fungus phycocolloid trapping and killing granules prepared by the invention are safe to human and other organisms, lasting in prevention and treatment effect, non-toxic and residue-free, high in specificity of pest control, easy to coordinate with prevention and treatment measures, capable of saving energy and capable of realizing green operation of the bamboo industry.

Description

Technical Field

[0001] The invention belongs to the technical field of biological pesticides, and particularly relates to a green muscardine alginate trapping granule for controlling wireworms in bamboo forests and a preparation method thereof. Background Art

[0002] Wireworms are a general term for the larvae of the family Elateridae (Coleoptera, Polyphaga, Elateridae). They harm a variety of crops such as wheat and corn, as well as trees, Chinese medicinal materials and forage. They mostly feed on the underground parts of plants and are an extremely important type of underground pest. Wireworms have a long life cycle, live underground, cause harm for a long time, are highly concealed, and their activity depth changes with changes in the external environment.

[0003] In southern China, wireworms are one of the most important pests in bamboo forests during the shoot period. They cause serious damage to the underground parts of bamboo and feed on bamboo shoots during the shoot period, which reduces the market value of bamboo shoots and causes the death of seed shoots, seriously restricting the regeneration of bamboo forests. The control of wireworms in bamboo forests has become an urgent problem to be solved in production.

[0004] Biological control has the advantages of being environmentally friendly, safe for humans and other organisms, having long-lasting control effects, being non-toxic and residue-free, having strong specificity in pest control, being easy to coordinate with other prevention and control measures, and being able to save energy. With the enhancement of people's environmental awareness and the increase in requirements for food safety, biological control is playing an increasingly important role in integrated pest control.

[0005] Biological control has received great attention in recent years. Metarhizium is one of the most studied and applied entomogenous fungi in the world. A variety of Metarhizium preparations for the control of grassland locusts and grubs have been developed at home and abroad. However, the research on the pathogenicity and control of Metarhizium to wireworms is rarely reported at home and abroad. In recent years, Kabuluk et al. systematically studied the control effect of Metarhizium on potato wireworms, Agriotes obscurus, and the results of field tests showed that it has a significant toxic effect. However, the indoor pathogenicity test of this strain on wireworms in bamboo forests in my country is not obvious. Metarhizium has a strong specificity for its host, so it is crucial to isolate a biological control strain with strong pathogenicity against wireworms in bamboo forests in my country, which is the basis and key to solving the biological control of wireworms. Summary of the invention

[0006] In view of the problems existing in the prior art, the purpose of the present invention is to provide a green anisopliae alginic acid entrapment granule for controlling bamboo wireworms and a preparation method, which is specifically achieved through the following technical solutions: A green muscardine alginate killing granule for controlling wireworms in bamboo forests. Every 100 ml of water contains the following components by weight: 1-3 g of sodium alginate, 0.7-0.8 g of green muscardine spore powder, 1-3 g of yeast, 7-9 g of attapulgite, and 0.3-0.7 g of rice flour.

[0007] Furthermore, every 100 ml of water contains the following components by weight: 2 g of sodium alginate, 0.75 g of green muscardine spore powder, 2 g of yeast, 8 g of attapulgite, and 0.5 g of rice flour.

[0008] Furthermore, the Metarhizium is the WP08 strain of Metarhizium pingshaense, and its preservation number is: CGMCC No.22474.

[0009] Furthermore, the attapulgite is selected from 1250 mesh attapulgite.

[0010] A method for preparing a green anisopliae alginic acid entrapment granule for controlling wireworms in bamboo forests, the method comprising the following steps: 1) Add 1-3 g of sodium alginate, 0.7-0.8 g of Metarhizium anisopliae spore powder, 1-3 g of yeast, 7-9 g of attapulgite, and 0.3-0.7 g of rice flour to 100 ml of water, mix and stir evenly to obtain a stirring solution; 2) Use a micro-injection pump to absorb the stirred liquid and drip it drop by drop into a 0.2 mol / L calcium chloride solution, control the particle size to 6 mm, form alginate particles, let it stand for solidification, wash with clean water for 2-3 minutes, filter it and place it on a clean tray to dry, and obtain the green muscardine alginate killing granules.

[0011] Furthermore, the flow rate of the microinjection pump is 400 mL / h.

[0012] Furthermore, in step 2), the mixture is allowed to stand for 15-25 minutes and the drying time is 2-3 days.

[0013] The green muscardine alginate trapping granules prepared by the present invention are safe to humans and other organisms, have a lasting control effect, are non-toxic and have no residue, have strong specificity for pest control, are easy to coordinate with other control measures, can save energy, and can realize green management of the bamboo industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is the effect diagram of the green muscardine algae granules in the forest to prevent wireworm damage; Figure 2 This is a diagram showing the effect of using green muscardine algae granules to control wireworm population density in the forest; Figure 3 This is a comparison chart of the effect of green muscardine alginate granules on controlling wireworms and bamboo shoots in the forest. DETAILED DESCRIPTION

[0015] The present invention is further described below in conjunction with specific embodiments to facilitate a better understanding of the present technical solution.

[0016] Example: Preparation of Metarhizium anisopliae alginic acid trapping granules Prepare the spore powder, filling carrier and nutrient activator required for the experiment, add 0.75% green muscardine spore powder, 2% yeast, 8% 1250 mesh attapulgite, and 0.5% rice flour to 2% sodium alginate solution, then use a micro-injection pump (instrument model LINZ-9A, flow rate 400 mL / h) to absorb the stirring liquid and drip it drop by drop into 0.2 mol / L calcium chloride solution to control the particle size to 6 mm to form alginate particles. After standing for 20 min to solidify, wash back and forth with clean water for 2 to 3 min, filter and place in a clean tray to dry for 2-3 days to form dry green muscardine alginate granules.

[0017] Preparation of yeast granules: The preparation method is the same as that of the Metarhizium alginate killing granules, the only difference is that no Metarhizium spore powder is added during the preparation process.

[0018] Preparation of Metarhizium granules: The preparation method is the same as that of Metarhizium alginate killing granules, the only difference is that no yeast is added during the preparation process.

[0019] Test Example 1: Spore production of three granules The spore production of three granules was measured under the same conditions, and the results are shown in Table 1.

[0020] Table 1: Sporulation of three granules

[0021] Test Example 2: Selection behavior of wireworms on three granules Place 1.75 kg of sterile soil with a humidity of 10% into the two-way selector, shake the wooden box horizontally to make the soil surface flat and the soil texture consistent in each part, and set aside.

[0022] Take 20 granules of yeast, green anisopliae granules and green anisopliae alginic trapping granules, and bury them in the bait chamber on either side of the two-way selection instrument, and leave the other bait chamber as a control. At the same time, put 10 wireworms in the sterile soil of the central insect chamber for testing and remove the partition. After 24 hours, reinstall the previously removed partition. First, count the number of wireworms in the central insect chamber (marked as "no choice"), and then count the number of wireworms in the bait chambers on both sides. The experiments were carried out under dark conditions (indoor temperature 25 ± 1℃, relative humidity 80% ± 10%), with a total of 12 replicates for each treatment. The comparison of the luring effect of the three granules on wireworms is shown in Table 2.

[0023] Table 2: Comparison of the attracting effects of three granules on wireworms

[0024] The results showed that the total number of larvae captured in the bait chamber containing the green anisopliae alginate granules was 52, accounting for 66.67% of the selected insects, and an average of 4.33±0.38 larvae were captured in each bait chamber (GP=8.83, P<0.005; GH=8.11, P=0.703). The total number of larvae captured in the bait chamber containing the yeast granules was 53, accounting for 72.60% of the selected insects, and an average of 4.42±0.49 larvae were captured in each bait chamber (G P=15.47, P<0.001; GH=23.06, P=0.017); the total number of sieve-chest comb-claw beetle larvae captured in the bait chamber where the Metarhizium granules were used was 27, accounting for 38.57% of the number of selected insects, and an average of 2.25±0.33 were captured in each bait chamber (GP=3.69, P>0.05; GH=20.43, P=0.039); Metarhizium alginate granules and yeast granules had a significant effect on the selection behavior of the sieve-chest comb-claw beetle larvae.

[0025] Test Example 3: Application of Metarhizium anisopliae Alginic Acid Trapping Granules in Forests The pesticide application method is ditch application, with a ditch width of 20 cm, a ditch spacing of 80-100 cm, and a ditch depth of 20-30 cm. The attractant is applied into the ditch in early May and late October to early November, with a single application amount of 20 kg / hm2 to 40 kg / hm2 and covered with soil. The demonstration results show that the comprehensive prevention and control technology in each demonstration area is effective in controlling bamboo wireworms. Taking the Fuyang demonstration area as an example, after two years of continuous control, the wireworm damage rate in the Fuyang demonstration area has dropped from the original 65.24% to 8.20% ( Figure 1 As shown in the figure, the rate of shoot rejection dropped to 15.38%, and the insect population density also dropped from 0.93 per plant to 0.42 per plant ( Figure 2 The control effect is the comparison of bamboo shoots withdrawal. Figure 3 The damage caused by wireworms was basically controlled in the demonstration area, ensuring the normal regeneration of the bamboo forest.

Claims

1. A green anisopliae alginic acid entrapment granule for controlling bamboo wireworms, characterized in that Every 100 ml of water contains the following components by weight: 1-3 g of sodium alginate, 0.7-0.8 g of green muscardine spore powder, 1-3 g of yeast, 7-9 g of attapulgite, and 0.3-0.7 g of rice flour.

2. The green anisopliae alginic acid entrapment granule for controlling bamboo wireworms according to claim 1, characterized in that Every 100 ml of water contains the following components by weight: 2 g of sodium alginate, 0.75 g of green muscardine spore powder, 2 g of yeast, 8 g of attapulgite, and 0.5 g of rice flour.

3. A green anisopliae alginic acid entrapment granule for controlling bamboo wireworms as claimed in claim 1 or 2, characterized in that The Metarhizium pingshaense is the WP08 strain of Metarhizium pingshaense, and its preservation number is: CGMCC No.22474.

4. A Metarhizium anisopliae alginic acid entrapment granule for controlling wireworms in bamboo forests as claimed in claim 1 or 2, characterized in that The attapulgite used was 1250 mesh attapulgite.

5. The method for preparing the green anisopliae alginic acid entrapment granules for controlling bamboo wireworms according to any one of claims 1-2, characterized in that The preparation method comprises the following steps: 1) Add 1-3 g of sodium alginate, 0.7-0.8 g of Metarhizium anisopliae spore powder, 1-3 g of yeast, 7-9 g of attapulgite, and 0.3-0.7 g of rice flour to 100 ml of water, mix and stir evenly to obtain a stirring solution; 2) Use a micro-injection pump to absorb the stirred liquid and drip it drop by drop into a 0.2 mol / L calcium chloride solution, control the particle size to 6 mm, form alginate particles, let it stand for solidification, wash with clean water for 2-3 minutes, filter it and place it on a clean tray to dry, and obtain the green muscardine alginate killing granules.

6. The method for preparing the green anisopliae alginic acid entrapment granules for controlling bamboo wireworms as claimed in claim 5, characterized in that The flow rate of the microsyringe pump was 400 mL / h.

7. The method for preparing the green anisopliae alginic acid entrapment granules for controlling bamboo wireworms as claimed in claim 5, characterized in that In step 2), let it stand for 15-25 minutes and the drying time is 2-3 days.

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