Animal polypeptide compound children's drink and preparation method thereof

Abstract

The present invention relates to an animal polypeptide composite children's drink and a preparation method thereof, and belongs to the technical field of health care products. The drink is divided into two types: day drink and night drink. The main raw materials of the day drink are composed of fried hawthorn, fried malt, Poria cocos, licorice and Codonopsis pilosula; the main raw materials of the night drink are composed of Poria cocos, jujube, spiny jujube kernel, licorice and Codonopsis pilosula. Both drinks are added with appropriate amounts of stevia liquid, monk fruit liquid, black chicken peptide and purified water, etc., a total of seven natural plant raw materials with medicinal and edible homology and three nutritional enhancers. The drink is specially designed for children and contains beneficial ingredients that promote digestion, enhance spleen and stomach function and accelerate growth and development, such as vitamin B2, vitamin C, carotene and a variety of organic acids, which can promote the secretion and activity of gastric digestive enzymes, improve digestion efficiency, and provide necessary nutritional support for children. Long-term drinking helps to alleviate children's picky eating, partial eating and other bad eating habits, prevent food accumulation and indigestion, and promote healthy growth.

Description

Technical Field

[0001] The present invention belongs to the technical field of health care products, and more specifically, relates to an animal polypeptide compound children's drink and a preparation method thereof. Background Art

[0002] During childhood, the rapid growth and development of the body and mind is particularly critical. During this important period, the health of the spleen and stomach of children becomes particularly important, because it is not only related to the absorption and utilization of nutrients by children, but also directly related to the overall health level of children. The spleen and stomach occupy a core position in traditional Chinese medicine theory, and are mainly responsible for transporting and transforming the essence of water and grain to provide the body with sufficient nutrition and energy.

[0003] In childhood, because the spleen and stomach functions are not fully developed, they are more susceptible to the influence of external environment and diet, leading to spleen and stomach dysfunction, which in turn causes a series of health problems. Therefore, having a good sleep quality is essential for the physical and mental health of children's growth. Good sleep also helps the body repair and regenerate, plays a key role in the development of the brain and the improvement of memory, and can also help children maintain emotional stability and enhance their attention and concentration.

[0004] Black chicken peptide is a bioactive peptide extracted from black chicken, which is rich in nutrients, including a variety of amino acids, trace elements and vitamins. Studies have shown that appropriate supplementation of black chicken peptide can promote the growth and development of children's bones and reduce the occurrence of accidents such as fractures. The various amino acids and trace elements in black chicken peptide have the effect of enhancing immunity, which can help children improve their resistance and reduce the occurrence of diseases. Especially in the season change and the high incidence of viruses, appropriate supplementation of black chicken peptide can effectively prevent common diseases such as colds. The various nutrients in black chicken peptide, especially cephalin and lecithin, have a positive effect on the development of children's nervous system and brain. These substances can promote the connection and communication of neurons and improve children's intelligence level and learning ability. Black chicken peptide also has the effect of improving the function of the digestive system and can help children better absorb nutrients. By promoting gastrointestinal motility and increasing the secretion of digestive juices, black chicken peptide can relieve children's indigestion, constipation and other problems, making their bodies healthier. However, there are no reports of nutritional products that combine medicine and food with black chicken peptide on the current market.

[0005] The present invention aims to develop a series of black chicken peptide drinks designed to address the problems that exist in children's growth process. Based on the classic Chinese medicine prescriptions and the concept of "medicine and food are of the same origin", we are committed to promoting the research and development and promotion of black chicken peptide drinks. Through scientific formulas and strict production processes, we hope to provide children with a drink that is both delicious and healthy, helping children get better nutritional support during their growth process, thereby promoting the all-round development of children. Summary of the invention

[0006] The purpose of the present invention is to provide an animal polypeptide compound children's drink, which is made from natural plants that are both medicinal and edible, and is added with a variety of trace elements necessary for the healthy growth of children, so as to strengthen the spleen and stomach, eliminate food accumulation, and promote growth.

[0007] The technical solution of the present invention is as follows:

[0008] An animal polypeptide compound children's drink, including a day drink and a night drink; wherein each 20 mL of the day drink includes: 9.35-12.65 mL of a day drink Chinese medicine liquid, 0.425-0.575 mL of a 20 mg / mL stevia liquid, 1.275-1.725 mL of a 20 mg / mL monk fruit liquid, 2.125-2.875 mL of a black chicken peptide, and the remainder of water;

[0009] Among them, by weight, each 11 mL of daily drinking Chinese medicine liquid includes: 0.425-0.575 g of stir-fried hawthorn, 0.212-0.288 g of stir-fried malt, 0.212-0.288 g of Poria cocos, 0.128-0.172 g of licorice, and 0.128-0.172 g of Codonopsis pilosula;

[0010] Each 20mL Night Drink contains: Night Drink Chinese Medicine Solution 9.35-12.65mL, 20mg / mL Stevia Solution 0.425-0.575mL, 20mg / mL Monk Fruit Solution 1.275-1.725mL, Black Chicken Peptide 2.125-2.875mL, and the rest is water;

[0011] Among them, in terms of weight, every 11 mL of the Chinese medicine liquid for night drinking includes: 0.425-0.575 g of Poria cocos, 0.212-0.288 g of jujube, 0.212-0.288 g of Ziziphus jujuba seeds, 0.128-0.172 g of licorice, and 0.128-0.172 g of Codonopsis pilosula.

[0012] In the present invention, the stevia liquid is prepared by brewing 2g of stevia with 100ml of boiling water; the added amount (ml) and the monk fruit liquid are prepared by brewing 2g of monk fruit with 100ml of boiling water.

[0013] Furthermore, each 20 mL of the daily drink includes: 11 mL of daily Chinese medicine liquid, 0.5 mL of stevia liquid, 1.5 mL of monk fruit liquid, 2.5 mL of black chicken peptide, and the remainder of water.

[0014] Furthermore, in terms of weight, every 11 mL of the daily drinking Chinese medicine liquid includes: 0.5 g of stir-fried hawthorn, 0.25 g of stir-fried malt, 0.25 g of Poria cocos, 0.15 g of licorice, and 0.15 g of Codonopsis pilosula.

[0015] Furthermore, the preparation method of the daily drinking Chinese medicine liquid comprises: washing and drying the stir-fried hawthorn, stir-fried malt, Poria cocos, licorice and Codonopsis pilosula; crushing them to less than 100 meshes, adding water and decocting them into the daily drinking Chinese medicine liquid.

[0016] The daily Chinese medicine liquid is improved from "Hawthorn Frost". This ancient recipe originated from "Taiping Shenghui Fang". It has been used to strengthen the spleen and appetite, digest food and eliminate accumulation in history. The formula has been adjusted on the basis of retaining the essence of the original recipe. Specifically, Codonopsis pilosula, which has the effects of tonifying the spleen and lungs, nourishing blood and promoting body fluid, is used to replace tangerine peel. Codonopsis pilosula can not only enhance the function of the spleen and stomach, but also help the health of the lungs, while replenishing blood and body fluids, which has a positive effect on the growth and development of children. In the recipe, fried hawthorn is the main medicine, which can effectively solve children's spleen and stomach problems because of its effects of digesting food and strengthening the stomach, promoting qi and dispersing blood stasis. Fried malt is the minister medicine, and its effects of promoting qi and digestion, strengthening the spleen and appetizing can further enhance the efficacy of hawthorn. Poria cocos is the adjuvant, and its effects of diuresis, invigorating the spleen, and calming the mind can reconcile the entire formula and eliminate possible side effects. Licorice and Codonopsis pilosula are the guiding medicines, which play the roles of tonifying the spleen and qi, clearing away heat and detoxifying, and tonifying the spleen and lungs, nourishing blood and promoting body fluid, respectively, guiding other medicines to play a better role. Finally, the dosage values ​​of each raw material in each 11mL of night-drinking Chinese medicine liquid were determined, specifically: 0.5g of stir-fried hawthorn, 0.25g of stir-fried malt, 0.25g of Poria cocos, 0.15g of licorice, and 0.15g of Codonopsis pilosula. In actual use, the dosage value of each raw material is ±15% based on the optimal formula. Through such a combination, a safe and effective Chinese medicine formula for strengthening the spleen and appetizing can be provided for children, helping them maintain the health of the spleen and stomach during their growth, thereby better absorbing nutrients and promoting the improvement of overall health levels.

[0017] Furthermore, each 20 mL of the night drink includes: 11 mL of night drink Chinese medicine solution, 1.0 mL of stevia solution, 0.5 mL of monk fruit solution, 2.5 mL of black chicken peptide, and the remainder of water.

[0018] Furthermore, in terms of weight, each 11 mL of the night drink Chinese medicine liquid includes: 0.5 g of Chinese jujube kernel, 0.25 g of Poria cocos, 0.25 g of jujube, 0.15 g of licorice, and 0.15 g of Codonopsis pilosula.

[0019] Furthermore, the preparation method of the Chinese medicine liquid for drinking at night includes: washing and drying Poria cocos, licorice, Codonopsis pilosula, jujube, and Ziziphus jujuba seeds; crushing to less than 100 meshes, adding water and decocting to obtain the Chinese medicine liquid for drinking at night.

[0020] Night is an important time for children to have a good sleep quality, and measures to clear heat and calm the nerves can help children have a good sleep quality. In order to achieve this goal, this formula is based on the famous ancient prescriptions for calming the nerves recorded in the "Golden Chamber Synopsis" "Suanzaoren Decoction" and "Ganmai Dazao Decoction". In the prescription, Suanzaoren, as the main medicine, enters the meridians of the heart and liver with its sweet, sour and moist properties, nourishes the heart and liver, and calms the mind. In addition, jujube and Poria are used as ministerial medicines. Jujube is used to nourish blood and calm the nerves, and Poria is used to promote diuresis and reduce swelling, calm the mind and calm the nerves. Codonopsis is used as a guiding medicine to nourish the spleen and lungs, nourish blood and produce body fluids. Licorice, as an adjuvant, plays a role in harmonizing the middle and relieving urgency and harmonizing various medicines. Through careful preparation and combined with the dosage standard, the dosage values ​​of each raw material in each 11ml of the final night drink Chinese medicine liquid are: Suanzaoren 0.5g, Poria 0.25g, jujube 0.25g, licorice 0.15g, and Codonopsis 0.15g. In actual use, the dosage of each raw material is ±15% based on the optimal formula. Such a ratio can better play its role in calming the mind, nourishing the heart, tonifying the spleen and benefiting the lungs, and provide children with a high-quality sleeping environment that is conducive to their physical and mental health.

[0021] The present invention also provides a method for preparing the above-mentioned animal polypeptide composite children's beverage, which specifically comprises the following steps: homogenizing, sterilizing, filling and sterilizing the liquid material according to the formula to obtain the beverage.

[0022] Furthermore, the sterilization is specifically as follows: using ultra-high temperature instantaneous sterilization equipment to sterilize the liquid feed, the sterilization temperature is set at 110-125° C., and the sterilization time lasts for 12-20 seconds.

[0023] Furthermore, the sterilization is specifically as follows: the filled product is immediately placed in a sterilization autoclave at 85-90° C. for 30-45 minutes, and cooled to room temperature after the sterilization is completed.

[0024] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0025] The present invention is guided by the theory of "medicine and food are of the same origin" in traditional Chinese medicine, selects raw materials such as roasted malt, Poria cocos, licorice, Codonopsis pilosula, jujube and Ziziphus jujuba seeds, and uses black chicken peptide as an auxiliary material. After careful blending, a plant-derived functional beverage is made that has significant effects on strengthening the spleen and stomach, eliminating food accumulation, and promoting growth for children.

[0026] The ingredients in the day and night drinks are not only rich and varied, but each ingredient has unique health benefits, working together on the human body to achieve multiple effects such as promoting digestion, enhancing immunity, improving sleep and learning memory. Long-term drinking can effectively improve symptoms such as picky eating, partial eating, easy food accumulation, indigestion, slow growth, poor vision development, etc., and can supplement the peptide protein and carotene, vitamins and other trace elements required for children's healthy growth.

[0027] The present invention adopts a unique formula design. The drink not only takes into account the nutrients that may be lacking in children's daily diet, but also optimizes the special needs of children's growth and development. The day drink mainly strengthens the supplement of vitamins and minerals, which helps to improve children's immunity and promote bone health; while the night drink focuses on improving sleep quality and promoting brain development, and contains ingredients that help relax nerves and improve sleep quality. Through the reasonable combination of daytime and nighttime drinks, the physiological needs of children in different time periods can be fully met, thereby achieving the best health promotion effect. In addition, the taste of the drink has been carefully formulated, which not only meets children's pursuit of deliciousness, but also ensures the natural health of the product, which is easy for children to accept. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 These are photos of the daytime drink and nighttime drink in the animal polypeptide compound drink provided by the present invention. DETAILED DESCRIPTION

[0029] Many specific details are set forth in the following description to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited to the specific implementation disclosed below.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention. It should be noted that the black chicken peptide in the embodiments of the present invention is provided by Hunan Ye Yi Yan Medical Technology Co., Ltd., and the other reagents used are all common commercial products.

[0031] The sensory evaluation standards used in the present invention are shown in Table 1.

[0032] Table 1 Sensory evaluation standards

[0033]

[0034] The specific evaluation process of the sensory evaluation of daytime and nighttime beverages is as follows:

[0035] 1. Select personnel with professional tasting ability and experience, and select 10 male and female college students majoring in food science and engineering, pharmacy and traditional Chinese medicine to ensure the objectivity and accuracy of the evaluation. The reviewers should have a good sense of smell and taste, and should not have colds, rhinitis or other diseases that affect the senses.

[0036] 2. Before evaluation, the reviewers should ensure that the product is tasted at the appropriate temperature. For different types of beverages, such as daytime and nighttime drinks, they should be tasted at the recommended appropriate drinking time to ensure the best taste and efficacy.

[0037] 3. Judges need to make a comprehensive evaluation based on the four indicators of color, aroma, taste and texture. First, observe the color of the drink to determine whether it meets the standard; then smell its aroma to assess whether there is a fishy smell or other odors; then taste the taste to feel the sourness and sweetness and taste coordination; finally, observe the texture to check whether there are solid particles and impurities. Each indicator is scored according to the standard, and the total score is finally calculated by combining the scores of each item.

[0038] Example 1 Screening of daily beverages

[0039] The L9 (3^4) orthogonal experimental design table was used to plan the experiment. Taking sensory scores as evaluation indicators, daily drinking Chinese medicine liquid, stevia (0.2g / L), and monk fruit (0.2g / L) were selected as the three factors A, B, and C of the study.

[0040] Three levels were set for each factor, and an orthogonal test of three factors and three levels was performed to determine the best parameter combination. A total of 10 sets of sensory scoring data were collected in this experiment. The setting of factor levels is based on Table 2. The specific arrangement of the experiment is based on Table 3 and Table 4. Each group of experiments was repeated three times, a total of 27 experiments were conducted, and all factors were statistically analyzed using SPSS17.0 software. At the end of each experiment, 50 mL of black chicken peptide was added, and purified water was added to a total volume of 300 mL.

[0041] Table 2 Factor level table of orthogonal experiment of daily beverage

[0042]

[0043] Table 3 Sensory analysis of daily beverages

[0044]

[0045]

[0046] Table 4 Analysis of variance of daily beverage data

[0047]

[0048] Note: F(0.01)=19.00, F(0.05)=99.00.

[0049] Result analysis: The higher the score, the better, so the optimal solution for the daily drink is A3B1C3, that is, 220mL of Chinese medicine liquid, 5mL of stevia, and 15mL of monk fruit. Therefore, the formula of the daily drink of the present invention is ±15% of the optimal solution.

[0050] Example 2 Screening of Night Drinks

[0051] The L9 (3^4) orthogonal experimental design table was used to plan the experiment. Taking the sensory score as the evaluation index, night drinking Chinese medicine liquid, stevia (0.2g / L), and monk fruit (0.2g / L) were selected as the other three factors A, B, and C of the study.

[0052] Three levels were set for each factor, and an orthogonal test of three factors and three levels was performed to determine the best parameter combination. A total of 10 sets of sensory scoring data were collected in this experiment. The setting of factor levels is based on Table 5. The specific arrangement of the experiment is based on Table 6 and Table 7. Each group of experiments was repeated three times, a total of 27 experiments were conducted, and all factors were statistically analyzed using SPSS17.0 software. At the end of each experiment, 50 mL of black chicken peptide was added, and purified water was added to a total volume of 300 mL.

[0053] Table 5 Factor level table of orthogonal test for night drinks

[0054]

[0055] Table 6 Sensory analysis of night drinks

[0056]

[0057] Table 7 Analysis of variance of night beverage data

[0058]

[0059] Note: F(0.01)=19.00, F(0.05)=99.00.

[0060] Result analysis: The higher the score, the better, so the optimal solution for night drinks is A3B2C1, that is, 220mL of Chinese medicine liquid, 10mL of stevia, and 5mL of monk fruit. Therefore, the formula of the daily drink of the present invention is ±15% of the optimal solution.

[0061] Preparation Example

[0062] The daily Chinese medicine liquid is prepared from Chinese medicinal materials such as hawthorn, malt, Poria cocos, licorice and Codonopsis pilosula. The night-drinking Chinese medicine liquid is prepared from fried Poria cocos, jujube, jujube kernel, licorice and Codonopsis pilosula. The washed and dried raw materials are then ultra-finely crushed by an ultra-fine grinder to ensure that the particle size can pass through a 100-mesh sieve. After that, the mixed Chinese medicinal materials are inspected to ensure that their quality meets the standards. After passing the inspection, they are cleaned and cleaned to remove impurities and dust on the surface of the medicinal materials. After cleaning, the medicinal materials are dried to remove moisture from the surface. The dried medicinal materials need to be soaked to better extract the effective ingredients. After soaking, the medicinal materials are decocted to extract the effective ingredients in the medicinal materials. Finally, the decocted day and night Chinese medicine liquid is filtered to remove impurities and obtain a pure Chinese medicine liquid.

[0063] Homogenization step: Use a 100MPa high-pressure homogenizer to homogenize the liquid to make the solution system more uniform and stable. Mix the day and night drink Chinese medicine liquid with the stevia liquid and monk fruit liquid, and mix them thoroughly to ensure that the various ingredients are evenly distributed. After mixing evenly, filter the mixture through a filtration device to remove tiny impurities. After filtering, add purified water to the mixture to adjust to the appropriate volume.

[0064] Liquid sterilization step: Use ultra-high temperature instantaneous sterilization equipment to sterilize the liquid, the sterilization temperature is set at 110-125°C, and the sterilization time lasts for 12-20 seconds;

[0065] Filling steps: Heat the liquid to 85-90℃, use hot can filling method, and sterilize immediately after capping. Transfer the adjusted volume of the mixed liquid to the filling equipment. Before filling, clean and disinfect the filling equipment to ensure the hygiene and safety of the filling process. After filling, cut and shape the filled product to meet the product specifications. After cutting and shaping, perform air tightness test and weight check on the product to ensure that the product's sealing and weight meet the standards.

[0066] Sterilization step: After removing foreign matter, place the sealed cans in an autoclave at 85-90°C for 30-45 minutes, and cool to 20-38°C after sterilization to ensure product safety and extend shelf life. After sterilization, the product can be packaged and stored as a finished product. Figure 1 shown.

[0067] After passing the air tightness test and weight check, the product is sent to the X-ray machine for foreign body removal test to ensure that there is no foreign matter in the product. The following is the nutritional composition table of this product:

[0068] The nutritional components of the compound beverage are shown in Tables 8 and 9.

[0069] Table 8 Nutritional composition of daily drinks

[0070] project Per 100g(g) Nutrient Reference Value% energy 287kJ 0％ protein 1.8g 3％ Fat 0g 0％ carbohydrate 15.1g 5％ sodium 6mg 0％

[0071] Table 9 Nutritional composition of night drinks

[0072] project Per 100g(g) Nutrient Reference Value% energy 235kJ 0％ protein 1.4g 2％ Fat 0g 0％ carbohydrate 12.3g 4％ sodium 3.6mg 0％

[0073] Hawthorn is an important ingredient in daily drinks, and it is rich in a variety of beneficial compounds. Specifically, hawthorn mainly contains flavonoids, flavanoids and their polymers, triterpenes, organic acids, lignans and steroids. These ingredients are not only rich, but also have many benefits for human health. In addition, hawthorn also contains vitamin B2, vitamin C, carotene and a variety of organic acids. The combined effect of these nutrients can promote the secretion of digestive enzymes in the stomach after eating hawthorn, and at the same time enhance the activity of enzymes, thereby promoting digestion. The pepsin agonist in hawthorn can enhance the activity of proteases and further strengthen the digestion process. At the same time, hawthorn also contains amylase, which can enhance the activity of pancreatic lipase, thereby promoting intestinal peristalsis and helping mechanical and chemical digestion. In addition, hawthorn also has an auxiliary protective and therapeutic effect on gastric mucosal damage, and can enhance the contraction of gastric and intestinal smooth muscles, thereby improving the overall function of the digestive system.

[0074] Poria polysaccharide is another important ingredient in daily drinks, which has the function of enhancing the immune system. Malt decoction, as a traditional herbal extract, can promote the secretion of gastric acid, pepsin and trypsin, thereby further promoting digestion. Licorice is also one of the key ingredients in daily drinks. It mainly contains triterpenoid saponins and flavonoids, which can enhance cellular immunity and improve digestive function. Codonopsis pilosula, as a precious herb, contains a variety of beneficial ingredients such as codonopsis pilosula and glucose, which can enhance immune function and improve learning and memory.

[0075] In night drinks, Poria polysaccharide also has an immune-enhancing effect. Malt decoction can promote the secretion of gastric acid, pepsin and trypsin, thereby promoting digestion. As an important ingredient, jujube polysaccharide can resist fatigue, promote bone marrow hematopoiesis, enhance immune function, effectively reduce intestinal peristalsis time, improve intestinal environment, and reduce intestinal mucosal contact with toxic substances and other harmful substances. Flavonoids have a sedative and hypnotic effect in night drinks, which helps to improve sleep quality. Ziziphus jujuba seeds mainly contain flavonoids, triterpenoid saponins and alkaloids, among which saponins mainly include jujube seed saponin A, B, and B1, which can improve sleep and enhance immunity after consumption. Licorice also contains triterpenoid saponins and flavonoids in night drinks, which can enhance cellular immunity and improve digestive function. Codonopsis pilosula also contains codonopsis glycosides, glucose and other ingredients in night drinks, which can enhance immune function and improve learning and memory.

[0076] The following animal experiments demonstrate the efficacy of day and night drinks.

[0077] Research on the functionality of daily beverages

[0078] This study used 60 SPF rats, all 3 weeks old and weighing between (60±10) g. These experimental animals were kept in an SPF animal room in the animal laboratory of Hunan University of Chinese Medicine. The ambient temperature was maintained at (23±3)°C and the relative humidity was maintained between 40% and 70%. The light cycle and dark cycle alternated every 12 hours to ensure that the experimental animals had access to sufficient food and drinking water.

[0079] Reagents and instruments:

[0080] Rat gastrin enzyme-linked immunosorbent assay (ELISA); rat motilin ELISA; rat β-endorphin ELISA; special medicated feed; conventional feed; Baohe Pills (Beijing Tongrentang Science and Technology Development Co., Ltd.).

[0081] Electronic balance, low-temperature desktop centrifuge, microplate reader, electric constant temperature drying oven.

[0082] According to Wang's etiology simulation method, an anorexia model of young rats was established. In the experiment, 10 rats were used as the control group and fed with conventional feed; the other 50 rats were used as the model group and given special feed. When the food intake of the model group rats was reduced to 40% to 60% of the control group and the body weight decreased by 10% to 15%, the model preparation was considered successful. After successful modeling, the model rats were randomly divided into a model control group, a high-dose daily drink group, a medium-dose group, a low-dose group and a Baohe pill group, with 10 rats in each group. All groups were fed with conventional feed and had free access to food and water.

[0083] Dosage

[0084] The daily drink high-dose group was 5.67g / (kg·d), the medium-dose group was 2.835g / (kg·d), and the low-dose group was 1.4175g / (kg·d); the Baohe pill group was also 2.835g / (kg·d). Baohe pills were ground into powder and sieved, dissolved in 0.5% sodium carboxymethylcellulose solution for later use. After grouping, rats in each group received drug gavage once a day. Rats in the control group and the model control group received gavage of 1 ml / day sodium carboxymethylcellulose solution, while rats in the high-, medium-, and low-dose groups of daily drinks and the Baohe pill group received gavage of corresponding doses of sodium carboxymethylcellulose powder suspension. After 14 days, the rats were euthanized.

[0085] Detection indicators and methods

[0086] Food intake and body weight of rats: The food intake and body weight of rats were recorded once at a fixed time every morning at 9:00. Gastric emptying rate and small intestinal propulsion rate of rats: After the last administration, rats were fasted but not watered for 24 hours. Before sampling, 1.5 mL / 100 g of charcoal nutritious semisolid paste was given by gavage. After intraperitoneal anesthesia, the gastric cardia and pylorus were ligated, and the rats were killed after cardiac blood was collected.

[0087] The stomach was removed and the surface moisture was absorbed with filter paper and then weighed; the stomach was cut open along the greater curvature of the stomach, the stomach contents were washed, the moisture was absorbed again and then weighed, and the gastric emptying rate was calculated using the formula: gastric emptying rate = (amount of charcoal-based nutritional semisolid paste administered intragastrically - (total stomach weight - net stomach weight)) / amount of charcoal-based nutritional semisolid paste administered intragastrically × 100%.

[0088] After taking out the stomach, quickly take out the small intestine, measure the distance from the pyloric sphincter to the point where the charcoal paste reaches as the advancement distance; the distance from the pyloric sphincter to the end of the ileum is the total length of the small intestine; calculate the small intestine advancement rate, the formula is: small intestine advancement rate (%) = advancement distance / total length of small intestine × 100%.

[0089] Rat serum GAS, MTL, and β-EP levels: Rat blood was collected using blood collection tubes and naturally coagulated at room temperature for 30 minutes. The supernatant was collected after centrifugation (1000×g, 15 minutes) and tested strictly according to the instructions of the GAS, MTL, and β-EP ELISA kits.

[0090] Test results

[0091] 1. Model Validation

[0092] After the model was successfully established, it was observed that the rats in each group were generally small in size, had a thinner subcutaneous fat layer, and tended to curl up in the corner of the cage. In addition, the responsiveness of these rats seemed to be weakened. Some rats had dull fur and even varying degrees of hair loss. In terms of behavior, the rats showed characteristics such as squinting, hunched backs, and irritability. However, after the end of the dosing cycle, the body size of the rats in each group increased significantly, the subcutaneous fat layer became thicker, the responsiveness was restored, the fur color became brighter, and the hair loss area improved. These changes together indicate that the model was successfully established.

[0093] 2. Effects of daily beverages on food intake and body weight of rats in each group

[0094] After drug intervention, the food intake of the blank group rats gradually stabilized, while the average food intake of the other groups showed an increasing trend. The body weight of rats in all groups showed a trend of continuous growth. On the 14th day of administration, the average food intake and body weight of the medium-dose group of the daily drink ranked first among all the drug-dosing groups. Compared with the model control group, the body weight of the medium-dose group of the daily drink increased significantly, and this difference was statistically significant. For detailed data, please see Table 10. (Food intake = (amount administered - remaining amount) / number of animals)

[0095] Table 10 Comparison of average food intake and average body weight of each group after administration

[0096]

[0097]

[0098] 3. Effects on gastric emptying rate and small intestinal propulsion rate in rats

[0099] Compared with the blank group, rats in the model control group showed significantly reduced gastric emptying rate and small intestinal propulsion rate, and this difference was statistically significant. In contrast, the gastric emptying rate of rats in the medium-dose daily drink group and the Baohe Pill group was significantly increased; the small intestinal propulsion rate of the medium-dose daily drink group was also significantly increased. Although the gastric emptying rate and small intestinal propulsion rate of rats in the other drug-administered groups increased, these differences did not reach statistical significance. Detailed data are shown in Table 11.

[0100] Table 11 Comparison of gastric emptying rate and small intestinal propulsion rate in rats of each group

[0101]

[0102] 4. Effects on serum gastrin (GAS), motilin (MTL), and β-endorphin (β-EP) levels in rats

[0103] Compared with the blank group, the levels of GAS, MTL, and β-EP in the model control group rats were significantly reduced, and these differences were statistically significant. In contrast, the levels of GAS and β-endorphin in the medium-dose group of the daily drink were significantly increased, and the difference was also statistically significant. Although the levels of GAS and β-EP in other drug-treated groups increased, the differences did not reach statistical significance. The serum motilin (MTL) levels in the high-dose, medium-dose, and low-dose groups of the daily drink were significantly increased, the differences were statistically significant, and significantly higher than the Baohe Pills group. Detailed data are shown in Table 12.

[0104] Table 12 Comparison of GAS, MTL and β-EP levels in rats of each group

[0105]

[0106] From the above results, it can be seen that the daily drink can significantly increase the levels of gastrin (GAS), motilin (MTL) and β-endorphin (β-EP) in serum. In particular, the effect on serum MTL levels is particularly significant, which helps to promote gastric emptying and small intestinal propulsion rate, thereby effectively improving anorexia symptoms. In addition, the drug can also increase food intake and improve body weight. It can be seen that the daily drink has a good effect on strengthening the spleen and appetite of children.

[0107] Functional study of night drinks

[0108] Instruments and Materials

[0109] Water maze system, UV-visible spectrophotometer, clean bench, low-temperature refrigerator, microplate reader, low-temperature refrigerated centrifuge, and homemade mouse sleep deprivation box.

[0110] Clean grade Kunming mice (18-22g), Anshenbunao solution, dopamine (DA) kit, 5-hydroxytryptamine (5-HT) kit.

[0111] Test Method

[0112] Sleep deprivation model establishment and grouping

[0113] At the Animal Experimental Center of Hunan University of Chinese Medicine, 60 clean-grade Kunming mice were randomly divided into 6 groups after 7 days of adaptive feeding. One group served as the normal control group and received oral gavage with normal saline. The other five groups were sleep deprived for 72 hours in a special sleep deprivation box, during which the mice were free to take in food and water. After the establishment of the sleep deprivation model, the five groups were further randomly divided into a negative control group, a positive control group, and low-, medium-, and high-dose groups of night drinks. The negative control group continued to receive oral gavage with normal saline, while the positive control group used Anshenbu Nao Oral Liquid (Jilin Aodong Yanbian Pharmaceutical Co., Ltd.) at 0.2mL·10g -1 The low, medium, and high dose groups of Night Drink received 2x, 5x, and 10x concentrated Night Drink by gavage at a dose of 0.2 mL for 15 days, during which the mice were free to drink water and eat.

[0114] Morris water maze test

[0115] After two weeks of continuous gavage treatment, the six groups of mice were subjected to a water maze test, which lasted for six days and was divided into two stages: a positioning navigation test and a spatial search test. During this process, the escape latency and the number of times the mice crossed the platform were recorded. The test data were expressed as mean and standard deviation (X±S).

[0116] Detection of DA and 5-HT content in mouse brain

[0117] After the mouse spine was dislocated, the brain was removed by decapitation. The whole brain was quickly taken out on an ice box, thoroughly rinsed with 4°C saline, dried with filter paper and weighed. Then, the homogenate was placed at 4°C and 3000 r / min. -1 Centrifuge for 10 minutes in a low-temperature refrigerated centrifuge, transfer the supernatant to a 1 mL EP tube, and store at -20°C for later use. Detection was performed using an enzyme-linked immunosorbent assay kit, and the OD values ​​of DA and 5-HT were determined using an enzyme-linked immunosorbent assay kit according to the instructions.

[0118] Test results

[0119] 1. Results of Morris water maze test in mice

[0120] In the Morris water maze test, the escape latency results of mice are shown in Table 13. There was no significant difference in the average escape latency of each group of mice on the first day of positioning navigation. However, as the number of training days increased, the average escape latency of all groups showed a downward trend. On the fifth day of training, the escape latency of mice showed a significant difference compared with the first day. In addition, the effect of the high-dose group of night drink on the escape latency of mice was almost the same as that of the positive control group on mice.

[0121] Table 13 Latency escape time of mice in water maze test

[0122]

[0123]

[0124] In the space exploration test on the sixth day, the number of times the mice crossed the platform is shown in Table 14. Compared with the positive control group and the high-dose night drink group, the number of times the mice in the negative control group crossed the platform was significantly lower, showing a statistically significant difference.

[0125] Table 14 Effect of night drinks on the number of times mice cross the platform

[0126]

[0127] 2. Results of DA content detection in mouse brain

[0128] According to the data analysis in Table 15, dopamine (DA) showed a very significant difference between the negative control group and the blank control group, which indicated that the model was successfully constructed. Further comparison showed that compared with the negative control group, the dopamine (DA) level in the low-dose group of night drinks increased and showed a significant difference; while the dopamine (DA) levels in the medium- and high-dose groups of night drinks and the positive control group increased significantly, all showing a very significant difference.

[0129] Table 15 Detection results of DA content in mouse brain

[0130]

[0131]

[0132] 3. Results of 5-HT content detection in mouse brain

[0133] According to the data analysis of Table 16, 5-hydroxytryptamine (5-HT) showed a very significant difference between the negative control group and the blank control group, which indicated that the model was successfully constructed. Further comparison showed that the level of 5-hydroxytryptamine (5-HT) in the low-dose group of the night drink decreased compared with the negative control group, and this change was statistically significant (P<0.05). In the medium- and high-dose groups of the night drink and the positive control group, the content of 5-hydroxytryptamine (5-HT) was significantly reduced, and the difference reached a very significant level (P<0.01). Considering the two indicators of dopamine (DA) and 5-hydroxytryptamine (5-HT), it can be concluded that the night drink prepared in this study showed a certain calming effect on sleep-deprived mice.

[0134] Table 16 Detection results of 5-HT content in mouse brain

[0135]

[0136] The above functional research test revealed that the night drink of the present invention can increase the dopamine (DA) level of sleep-deprived adult mice and significantly reduce the content of 5-hydroxytryptamine (5-HT). The effect shown by the high-dose group is comparable to that of the positive control group, which indicates that the drink has a certain calming and brain-tonifying effect on mice suffering from sleep deprivation. It can be seen that the night drink provided by the present invention has the effect of calming the mind and strengthening the brain.

[0137] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An animal polypeptide compound children's drink, characterized in that: Includes daytime drinks and nighttime drinks; each 20mL of daytime drinks includes: 9.35-12.65mL of daily Chinese medicine liquid, 0.425-0.575mL of stevia liquid, 1.275-1.725mL of monk fruit liquid, 2.125-2.875mL of black chicken peptide, and the rest of water; Among them, every 11mL of daily Chinese medicine liquid includes: stir-fried hawthorn 0.425-0.575g, stir-fried malt 0.212-0.288g, Poria 0.212-0.288g, licorice 0.128-0.172g, Codonopsis pilosula 0.128-0.172g; Each 20mL Night Drink contains: Night Drink Chinese Medicine Solution 9.35-12.65mL, Stevia Solution 0.425-0.575mL, Monk Fruit Solution 1.275-1.725mL, Black Chicken Peptide 2.125-2.875mL, and the rest is water; Among them, every 11mL of Chinese medicine solution for night drinking includes: 0.425-0.575g of Poria cocos, 0.212-0.288g of jujube, 0.212-0.288g of Ziziphus jujuba seeds, 0.128-0.172g of licorice, and 0.128-0.172g of Codonopsis pilosula.

2. The animal polypeptide compound children's drink according to claim 1, characterized in that: Every 20mL daily drink includes: 11mL of Chinese medicine liquid, 0.5mL of stevia liquid, 1.5mL of monk fruit liquid, 2.5mL of black chicken peptide, and the rest of the drink is water.

3. The animal polypeptide compound children's drink according to claim 1, characterized in that: Every 11 mL of Chinese medicine liquid for daily consumption includes: 0.5 g of stir-fried hawthorn, 0.25 g of stir-fried malt, 0.25 g of Poria cocos, 0.15 g of licorice, and 0.15 g of Codonopsis pilosula.

4. The animal polypeptide compound children's drink according to claim 1, characterized in that: Each 20mL of Night Drink includes: 11mL of Night Drink Chinese Medicine Liquid, 1.0mL of Stevia Liquid, 0.5mL of Monk Fruit Liquid, 2.5mL of Black Chicken Peptide, and the rest of the water.

5. The animal polypeptide compound children's drink according to claim 1, characterized in that: In terms of weight, every 11 mL of the night drink Chinese medicine liquid includes: 0.5 g of Poria cocos, 0.25 g of jujube, 0.25 g of Ziziphus jujuba seeds, 0.15 g of Licorice, and 0.15 g of Codonopsis pilosula.

6. The animal polypeptide compound children's drink according to claim 1, characterized in that: The preparation method of the daily drinking Chinese medicine liquid comprises: washing and drying fried hawthorn, fried malt, Poria, liquorice and Codonopsis; crushing to less than 100 meshes, adding water and boiling to obtain the daily drinking Chinese medicine liquid.

7. The animal polypeptide compound children's drink according to claim 1, characterized in that: The preparation method of the Chinese medicine liquid for drinking at night comprises: washing and drying Poria cocos, liquorice, Codonopsis pilosula, jujube and Ziziphus jujuba seeds; crushing to less than 100 meshes, adding water and boiling to obtain the Chinese medicine liquid for drinking at night.

8. The method for preparing the animal polypeptide compound children's drink according to any one of claims 1 to 7, characterized in that: The material liquid is homogenized, sterilized, filled and sterilized according to the formula.

9. The preparation method according to claim 8, characterized in that: The sterilization is specifically as follows: using ultra-high temperature instantaneous sterilization equipment to sterilize the liquid feed, the sterilization temperature is set at 110-125° C., and the sterilization time lasts for 12-20 seconds.

10. The preparation method according to claim 8, characterized in that: The sterilization is specifically as follows: the filled product is immediately placed in a sterilization autoclave at 85-90° C. for 30-45 minutes, and cooled to room temperature after the sterilization is completed.

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