A processing and preparation method of Cistanche deserticola decoction rich in seabuckthorn flavonoids

By extracting and mixing the active ingredients of sea buckthorn flavonoids and Cistanche, a drink liquid rich in sea buckthorn flavonoids and Cistanche polysaccharides was prepared, which solved the problem that existing products could not fully exert the comprehensive health benefits of both, and achieved significant antioxidant, immune enhancement, anti-fatigue and liver protection effects.

CN119732449APending Publication Date: 2025-04-01XINJIANG AILINUR CHAMAGU IND BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411475309.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-10-22
Publication Date
2025-04-01

AI Technical Summary

Technical Problem

There is a lack of combined application research on sea buckthorn and Cistanche on the existing market, resulting in the use of products using one of the ingredients alone, and the comprehensive health care effects of both are not fully utilized.

Method used

Through scientific and reasonable processes, the active ingredients of sea buckthorn flavonoids and Cistanche are extracted and mixed in a specific proportion to prepare a beverage rich in sea buckthorn flavonoids and Cistanche polysaccharides, including extraction, mixing, bactericidal and filling steps.

Benefits of technology

A new health drink with significant antioxidant, enhanced immunity, anti-fatigue, liver protection and excellent taste was developed to give full play to the comprehensive health care effects of sea buckthorn flavonoids and Cistanche.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to the technical field of health beverages, and specifically discloses a processing and preparation method for a seabuckthorn flavonoid-rich Cistanche decoction. The method comprises the following steps: S1: extracting flavonoids in seabuckthorn fruit and polysaccharides in Cistanche decoction respectively to obtain a seabuckthorn flavonoid extract and a Cistanche decoction polysaccharide extract; S2: mixing the seabuckthorn flavonoid extract, the Cistanche decoction polysaccharide extract, purified water, a sweetener and an acidity regulator in a mass fraction ratio of 20%-30%: 10%-20%: 50%-60%: 2%-5%: 0.1%-0.5%, and adjusting the pH value of the mixed solution to 5.5-6.5; S3: sterilizing the mixed solution obtained in S2 to obtain a drinking liquid. The invention organically combines the effective ingredients of seabuckthorn flavonoids and Cistanche decoction through a scientific extraction and proportioning method, and develops a drinking liquid rich in seabuckthorn flavonoids and Cistanche decoction polysaccharide, which has significant health care effects and excellent taste.
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Description

Technical Field

[0001] The invention relates to the technical field of health beverages, in particular to a method for processing and preparing a Cistanche deserticola drinking liquid rich in seabuckthorn flavonoids. Background Art

[0002] Seabuckthorn is a plant widely distributed in Eurasia. Its fruit is known as the "king of vitamins" and is rich in a variety of biologically active ingredients. Seabuckthorn fruit is rich in nutrients such as vitamin C, vitamin E, carotene, various amino acids and minerals, especially flavonoids, which are one of its main active ingredients. Seabuckthorn flavonoids have significant biological functions such as antioxidant, anti-inflammatory, liver protection, anti-radiation, lowering blood lipids and enhancing immunity. Seabuckthorn has been proven to have a variety of health benefits in traditional medicine and modern scientific research, including the treatment of digestive system diseases, cardiovascular diseases and skin diseases. In addition, seabuckthorn is also widely used in the food, pharmaceutical and cosmetic industries to make a variety of products such as seabuckthorn oil, seabuckthorn juice, seabuckthorn tea, etc.

[0003] Cistanche deserticola is a perennial herbaceous plant that parasitizes on the roots of the desert plant Haloxylon ammodendron. It is mainly distributed in Xinjiang and Inner Mongolia in China. Cistanche deserticola has been known as the "desert ginseng" since ancient times. It is well documented in traditional Chinese medicine books and has a variety of health benefits, including tonifying the kidney and strengthening yang, moistening the intestines and relieving constipation, enhancing physical strength and resisting fatigue. Its main active ingredients include phenylethanol glycosides (such as echinacoside and verbascoside) and polysaccharides. Modern research shows that Cistanche deserticola has a wide range of pharmacological effects, including anti-aging, antioxidant, anti-inflammatory, nerve protection and immunity enhancement. Cistanche deserticola polysaccharides are widely used in the development of health foods and functional beverages by regulating intestinal flora, improving immune function and anti-fatigue effects.

[0004] With the change of modern people's lifestyle and the enhancement of health awareness, the market demand for natural, green and functional health products is growing. Seabuckthorn and Cistanche deserticola are two natural plants with great health value, which are rich in flavonoids and polysaccharides respectively. However, there are few studies on the combined application of seabuckthorn and Cistanche deserticola in the market, and the products only use one of the ingredients separately. If the active ingredients of the two can be organically combined through scientific and reasonable processes, not only can their comprehensive health effects be fully exerted, but also new health drinks with market competitiveness can be developed. Summary of the invention

[0005] In order to solve the problems mentioned in the background technology, the object of the present invention is to provide a processing and preparation method of Cistanche deserticola decoction rich in seabuckthorn flavonoids.

[0006] In order to achieve the above object, the technical solution of the present invention is:

[0007] A method for preparing a Cistanche deserticola decoction rich in seabuckthorn flavonoids comprises the following steps:

[0008] S1: extracting flavonoids from seabuckthorn fruit and polysaccharides from Cistanche deserticola respectively to obtain seabuckthorn flavonoids extract and Cistanche deserticola polysaccharide extract;

[0009] S2: Mix the seabuckthorn flavonoids extract, the Cistanche deserticola polysaccharide extract, purified water, a sweetener and an acidity regulator in a mass fraction ratio of 20%-30%: 10%-20%: 50%-60%: 2%-5%: 0.1%-0.5%, and adjust the pH value of the mixture to 5.5-6.5;

[0010] S3: sterilize the mixed solution obtained in S2 to obtain a drinkable solution.

[0011] Furthermore, the preparation method of the seabuckthorn flavonoids extract in step S1 is: after the seabuckthorn fruit is crushed, reflux extraction is carried out at 50-60°C for 2 hours with 70% ethanol solution at a liquid-to-solid ratio of 10:1, and the extract is filtered, concentrated and dried to obtain a powdered seabuckthorn flavonoids extract.

[0012] Furthermore, the preparation method of the Cistanche polysaccharide extract in step S1 is: crush the Cistanche, soak it in 80-90°C water for 3 hours at a liquid-to-solid ratio of 20:1 to obtain an extract, filter the extract, concentrate and dry it to obtain a powdered Cistanche polysaccharide extract.

[0013] Furthermore, in step S3, the mixed solution is pasteurized at 85° C. for 15 minutes, and immediately cooled to room temperature after the pasteurization.

[0014] Furthermore, the sweetener is honey or xylitol or a mixture of the two.

[0015] Furthermore, the acidity regulator is citric acid.

[0016] Furthermore, the method further comprises step S4: filling and sealing the drinking liquid in a sterile environment.

[0017] The beneficial effects of the present invention are as follows: the present invention organically combines the effective ingredients of seabuckthorn flavonoids and Cistanche deserticola through scientific extraction and proportioning methods, and develops a drink rich in seabuckthorn flavonoids and Cistanche deserticola polysaccharides, which has significant health care effects and excellent taste. The specific beneficial effects are as follows: it has an antioxidant effect, and the polysaccharides in seabuckthorn flavonoids and Cistanche deserticola have strong antioxidant capacity, can effectively remove free radicals in the body, slow down cell aging, protect the cardiovascular system, and prevent the occurrence of chronic diseases; enhance immunity, and the combination of seabuckthorn flavonoids and Cistanche deserticola can significantly improve the body's immune function, and seabuckthorn flavonoids can enhance the body's resistance by enhancing the activity of immune cells; Cistanche deserticola Polysaccharides enhance the body's disease resistance by regulating the immune system; anti-fatigue effect: the active ingredients in the drink can improve the body's energy metabolism, enhance endurance and fatigue resistance, and help relieve fatigue caused by high work pressure and fast pace of life in modern people; liver protection: seabuckthorn flavonoids have a significant liver protection effect, which can promote the regeneration and repair of liver cells and prevent liver damage. At the same time, Cistanche deserticola also has a liver protection effect, which can reduce the burden on the liver and prevent liver disease; excellent taste: by adding sweeteners and acidity regulators, the drink has a good taste, which can not only maintain the natural flavor of the raw materials, but also meet the needs of modern consumers for healthy drinks. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a flow chart of an embodiment of the present invention. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field belong to the protection scope of the present invention.

[0020] The process flow chart of the present invention can be referred to Figure 1 .

[0021] Embodiment 1

[0022] Raw materials preparation:

[0023] 1000 g sea buckthorn fruit, processing: washing, peeling, deseeding, and crushing to obtain sea buckthorn fruit pulp;

[0024] 500 g of Cistanche deserticola was processed by washing, slicing, drying and crushing to obtain Cistanche deserticola powder.

[0025] Extraction of seabuckthorn flavonoids:

[0026] Prepare a 70% ethanol solution, take 7000 ml of anhydrous ethanol and mix it with 3000 ml of pure water to prepare a 70% ethanol solution. Put the seabuckthorn jam into the extraction container, and add 10000 ml of 70% ethanol solution according to the liquid-to-solid ratio of 10:1. Place the extraction container in a water bath, control the temperature at 50°C, and keep it for 2 hours. During this period, stir moderately to promote the dissolution of flavonoid compounds. After the extraction is completed, filter the mixed solution through a filter cloth or filter paper to remove solid residues to obtain a clear extract. Then place the extract in a rotary evaporator, evaporate and remove part of the ethanol under reduced pressure, concentrate the extract to 1 / 5 of the original volume, obtain a concentrated solution rich in seabuckthorn flavonoids, and further dry the concentrated solution to obtain seabuckthorn flavonoid powder. The dried powder is placed in a dry sealed container and stored at low temperature for standby use.

[0027] Extraction of Cistanche Polysaccharide:

[0028] The Cistanche powder is placed in an extraction container, and 10,000 ml of pure water is added at a liquid-to-solid ratio of 20:1. The extraction container is placed in a heating device, the temperature is controlled at 80-90°C, and maintained for 3 hours, during which moderate stirring is performed to promote the dissolution of Cistanche polysaccharides. After the extraction is completed, the mixed solution is passed through a filter cloth or filter paper to remove solid residues to obtain a clear extract. The extract is then placed in a rotary evaporator, and some water is evaporated and removed under reduced pressure, and the extract is concentrated to 1 / 5 of the original volume to obtain a concentrated solution rich in Cistanche polysaccharides. The concentrated solution is further dried to obtain Cistanche polysaccharide powder, and the dried powder is placed in a dry sealed container and stored at low temperature for standby use.

[0029] Mix seabuckthorn flavonoid powder, Cistanche deserticola powder and purified water evenly, then add appropriate amount of honey and citric acid and stir evenly, adjust the pH value of the mixture to 5.5-6.5, wherein the mass fraction ratio of seabuckthorn flavonoid powder, Cistanche deserticola powder, purified water, honey and citric acid is 25:15:55:4.8:0.2. After homogenization, the mixture is pasteurized to obtain a drinking liquid, the sterilization temperature is 85°C, the sterilization time is 15 minutes, and the sterilized drinking liquid is filled into a sterilized bottle in a sterile environment and quickly sealed. The sealed beverage bottle is stored at room temperature and avoids direct sunlight to ensure the quality and shelf life of the product.

[0030] Product indicators and test results:

[0031] 1. Physical and chemical indicators

[0032] pH value: measured with a pH meter, the pH value of the liquid is 5.8, which is within the drinking standard range and suitable for long-term storage;

[0033] Solid content: Determined by evaporation method, the solid content of the drink is 12%;

[0034] Soluble sugar content: measured by refractometer, the soluble sugar content of the drink is 6%;

[0035] Total acidity: Determined by acid-base titration, the total acidity of the drink is 0.35%.

[0036] 2. Nutritional content

[0037] Vitamin C content: Determined by high performance liquid chromatography (HPLC), the vitamin C content of the drinkable liquid is 150 mg / L;

[0038] Flavonoid content: measured by spectrophotometry, the flavonoid content of the drinking liquid is 80 mg / L;

[0039] Polysaccharide content: Determined by phenol-sulfuric acid method, the polysaccharide content of the drinking solution is 120 mg / L.

[0040] 3. Microbiological indicators

[0041] Total colony count: Determined by plate count method, the total colony count of the drinking liquid is less than 100 CFU / mL, which meets the national beverage standards;

[0042] Coliform bacteria: determined by multiple tube fermentation method, no coliform bacteria were detected in drinking water, which meets the national beverage hygiene standards;

[0043] Mold and yeast: The plate counting method was used for determination. No mold and yeast were detected in the drinking liquid, which meets the national beverage standards.

[0044] 4. Sensory indicators

[0045] Color: The liquid is light yellow, transparent and free of suspended matter;

[0046] Smell: It has the natural fragrance of sea buckthorn and Cistanche deserticola, without any peculiar smell;

[0047] Taste: Sweet taste, moderate acidity, refreshing in the mouth and long aftertaste.

[0048] 5. Stability test

[0049] High temperature stability: When the drink is stored at 37°C for 30 days, the content of the main components of the drink does not change much, and the drink has good stability;

[0050] Low temperature stability: When the drink is stored in a 0℃ environment for 30 days, the content of the main components of the drink does not change much, and the drink has good stability.

[0051] Through the above physical and chemical indicators, nutritional components, microbial indicators, sensory indicators and stability test results, it can be seen that the drinking liquid rich in seabuckthorn flavonoids and Cistanche polysaccharides has reached the expected quality standards and health effects in all aspects, and is suitable for being put on the market as a high-quality functional beverage.

[0052] Embodiment 2

[0053] 1500g sea buckthorn fruit, processing: washing, peeling, removing seeds, and crushing to obtain sea buckthorn fruit pulp;

[0054] 750 g of Cistanche deserticola was processed by washing, slicing, drying and crushing to obtain Cistanche deserticola powder.

[0055] Extraction of seabuckthorn flavonoids:

[0056] Prepare a 70% ethanol solution, take 10500 ml of anhydrous ethanol and mix it with 4500 ml of pure water to prepare a 70% ethanol solution. Put the seabuckthorn jam into the extraction container, and add 10000 ml of 70% ethanol solution according to the liquid-to-solid ratio of 10:1. Place the extraction container in a water bath, control the temperature at 50°C, and keep it for 2 hours. Stir moderately during the period to promote the dissolution of flavonoid compounds. After the extraction is completed, filter the mixed solution through a filter cloth or filter paper to remove solid residues to obtain a clear extract. Then place the extract in a rotary evaporator, evaporate and remove part of the ethanol under reduced pressure, concentrate the extract to 1 / 5 of the original volume, obtain a concentrated solution rich in seabuckthorn flavonoids, and further dry the concentrated solution to obtain seabuckthorn flavonoid powder. The dried powder is placed in a dry sealed container and stored at low temperature for standby use.

[0057] Extraction of Cistanche Polysaccharide:

[0058] The Cistanche powder is placed in an extraction container, and 15000 ml of pure water is added at a liquid-to-solid ratio of 20:1. The extraction container is placed in a heating device, the temperature is controlled at 80-90°C, and maintained for 3 hours, during which moderate stirring is performed to promote the dissolution of Cistanche polysaccharides. After the extraction is completed, the mixed solution is passed through a filter cloth or filter paper to remove solid residues to obtain a clear extract. The extract is then placed in a rotary evaporator, and part of the water is evaporated and removed under reduced pressure, and the extract is concentrated to 1 / 5 of the original volume to obtain a concentrated solution rich in Cistanche polysaccharides. The concentrated solution is further dried to obtain Cistanche polysaccharide powder, and the dried powder is placed in a dry sealed container and stored at low temperature for standby use.

[0059] The seabuckthorn flavonoids powder, the Cistanche deserticola powder and the purified water are mixed evenly, and then appropriate amounts of xylitol and citric acid are added and stirred evenly, and the pH value of the mixed solution is adjusted to 5.5-6.5, wherein the mass fraction ratio of the seabuckthorn flavonoids powder, the Cistanche deserticola powder, the purified water, the honey and the citric acid is 30:20:46.5:3:0.5. The mixed solution is homogenized and then pasteurized to obtain a drinking liquid, the sterilization temperature is 85°C, the sterilization time is 15 minutes, and the sterilized drinking liquid is filled into a sterilized bottle in a sterile environment and quickly sealed. The sealed beverage bottle is stored at room temperature and avoids direct sunlight to ensure the quality and shelf life of the product.

[0060] Product indicators and test results

[0061] 1. Physical and chemical indicators

[0062] pH value: measured with a pH meter, the pH value of the drinking solution is 5.9, which is within the drinking standard range and suitable for long-term storage;

[0063] Solid content: Determined by evaporation method, the solid content of the drink is 13%;

[0064] Soluble sugar content: measured by refractometer, the soluble sugar content of the drink is 7%;

[0065] Total acidity: Determined by acid-base titration, the total acidity of the drink is 0.4%.

[0066] 2. Nutritional content

[0067] Vitamin C content: Determined by high performance liquid chromatography (HPLC), the vitamin C content of the drinkable liquid is 150 mg / L;

[0068] Flavonoid content: measured by spectrophotometry, the flavonoid content of the drinking liquid is 85 mg / L;

[0069] Polysaccharide content: Determined by phenol-sulfuric acid method, the polysaccharide content of the drinking solution is 130 mg / L.

[0070] 3. Microbiological indicators

[0071] Total colony count: Determined by plate count method, the total colony count of the drinking liquid is less than 100 CFU / mL, which meets the national beverage standards;

[0072] Coliform bacteria: determined by multiple tube fermentation method, no coliform bacteria were detected in drinking water, which meets the national beverage hygiene standards;

[0073] Mold and yeast: The plate counting method was used for determination. No mold and yeast were detected in the drinking liquid, which meets the national beverage standards.

[0074] 4. Sensory indicators

[0075] Color: The liquid is light yellow, transparent and free of suspended matter;

[0076] Smell: It has the natural fragrance of sea buckthorn and Cistanche deserticola, without any peculiar smell;

[0077] Taste: Sweet taste, moderate acidity, refreshing in the mouth and long aftertaste.

[0078] 5. Stability test

[0079] High temperature stability: When the drink is stored at 37°C for 30 days, the content of the main components of the drink does not change much, and the drink has good stability;

[0080] Low temperature stability: When the drink is stored in a 0℃ environment for 30 days, the content of the main components of the drink does not change much, and the drink has good stability.

[0081] Through the above physical and chemical indicators, nutritional components, microbial indicators, sensory indicators and stability test results, it can be seen that the drinking liquid rich in seabuckthorn flavonoids and Cistanche polysaccharides has reached the expected quality standards and health effects in all aspects, and is suitable for being put on the market as a high-quality functional beverage.

[0082] In addition, the chemical composition analysis and biological activity determination of the drink obtained in Example 2 were also carried out.

[0083] Chemical composition analysis

[0084] In order to ensure that the drink rich in seabuckthorn flavonoids and Cistanche deserticola polysaccharides has the expected health benefits and quality, its chemical components were analyzed in detail. The following are the analysis results of the main chemical components and their contents:

[0085] 1. Vitamin C (ascorbic acid)

[0086] Content: 160 mg / L (Example 2)

[0087] Determination method: High performance liquid chromatography (HPLC)

[0088] Function: Vitamin C has a strong antioxidant effect, can enhance immunity, promote collagen synthesis and improve skin health.

[0089] 2. Flavonoids

[0090] Content: 85 mg / L (Example 2)

[0091] Determination method: spectrophotometry

[0092] Main ingredients: quercetin, kaempferol, isorhamnetin, etc.

[0093] Function: Flavonoids have antioxidant, anti-inflammatory, anti-allergic, cardiovascular and liver protective effects.

[0094] 3. Polysaccharides

[0095] Content: 130 mg / L (Example 2)

[0096] Determination method: phenol-sulfuric acid method

[0097] Main ingredients: glucan, arabinan, mannan, etc.

[0098] Function: Polysaccharide compounds have many health benefits such as enhancing immunity, anti-tumor, lowering blood sugar and anti-fatigue.

[0099] 4. Total acidity

[0100] Content: 0.4% (Example 2)

[0101] Determination method: acid-base titration

[0102] Function: Total acidity mainly reflects the acidity of drinking liquid, affecting the taste and shelf life.

[0103] 5. Soluble sugar

[0104] Content: 7% (Example 2)

[0105] Determination method: Refractometer

[0106] Main ingredients: glucose, fructose, sucrose, etc.

[0107] Function: Provide sweetness, improve the taste of drinks, and provide energy.

[0108] 6. Solid content

[0109] Content: 13% (Example 2)

[0110] Determination method: Evaporation method

[0111] Function: Solid content mainly reflects the concentration of drinking water and the total amount of nutrients.

[0112] 7. Trace Elements

[0113] Main ingredients: potassium, sodium, calcium, magnesium, iron, zinc, etc.

[0114] Determination method: Atomic absorption spectrometry (AAS)

[0115] Function: Trace elements play an important role in human physiological activities and are involved in enzyme activation, metabolic regulation and bone health.

[0116] 8. Phenolic compounds

[0117] Content: A small amount exists in the determination

[0118] Determination method: High performance liquid chromatography (HPLC)

[0119] Function: Phenolic compounds have antioxidant, antibacterial, antiviral and other effects, enhancing the health function of drinking liquids.

[0120] Through the above chemical composition analysis, it can be determined that the drink rich in seabuckthorn flavonoids and Cistanche deserticola polysaccharides contains a variety of active ingredients that are beneficial to human health. These ingredients not only provide the drink with a variety of health functions, but also ensure its good taste and nutritional value.

[0121] Biological activity assay

[0122] In order to evaluate the health benefits of the drink rich in seabuckthorn flavonoids and Cistanche deserticola polysaccharides, a number of biological activity tests were conducted. The following are the main biological activities and their test results:

[0123] 1. Antioxidant Activity

[0124] Determination method: DPPH free radical scavenging method and ABTS free radical scavenging method.

[0125] result:

[0126] DPPH free radical scavenging rate: At a concentration of 0.5 mg / mL, the DPPH free radical scavenging rate of the drinking solution was 85%.

[0127] ABTS free radical scavenging rate: At a concentration of 0.5 mg / mL, the ABTS free radical scavenging rate of the drink was 90%.

[0128] Function: It has strong antioxidant activity and can effectively remove free radicals in the body, slow down cell aging, and protect the cardiovascular system.

[0129] 2. Immune-enhancing activity

[0130] Determination method: Mouse spleen cell proliferation experiment and macrophage phagocytic activity assay.

[0131] result:

[0132] Spleen cell proliferation rate: At a concentration of 100 μg / mL, the drink can significantly promote the proliferation of mouse spleen cells, with a proliferation rate of 50%.

[0133] Macrophage phagocytic activity: The drink can significantly increase the phagocytic activity of macrophages, and the phagocytic rate increases by 30%.

[0134] Function: The drink has a significant immune-enhancing effect, can improve the body's immunity and enhance disease resistance.

[0135] 3. Anti-fatigue activity

[0136] Determination method: Mouse swimming endurance test and blood lactate determination.

[0137] result:

[0138] Swimming endurance time: The swimming endurance time of mice in the drinking solution treatment group was significantly prolonged, 40% longer than that in the control group.

[0139] Blood lactate level: The blood lactate level of mice in the drinking water treatment group after swimming was significantly reduced, 30% lower than that in the control group.

[0140] Function: The drink has a significant anti-fatigue effect and can improve physical strength and relieve fatigue.

[0141] 4. Anti-inflammatory Activity

[0142] Measurement method: Mouse ear swelling model and cytokine (such as TNF-α, IL-6) measurement.

[0143] result:

[0144] Ear swelling inhibition rate: The drink can significantly inhibit ear swelling in mice, with an inhibition rate of 60%.

[0145] Cytokine levels: Drinking the liquid can significantly reduce the levels of inflammatory factors TNF-α and IL-6.

[0146] Function: The drink has significant anti-inflammatory effects, can reduce inflammatory responses and relieve inflammatory symptoms.

[0147] 5. Hepatoprotective activity

[0148] Determination method: Carbon tetrachloride (CCl4)-induced acute liver injury model in mice, and detection of serum ALT and AST levels.

[0149] result:

[0150] Serum ALT and AST levels: The serum ALT and AST levels of mice in the drinking liquid treatment group were significantly reduced, 40% lower than those in the control group.

[0151] Function: The drink has a significant liver-protective effect, can reduce liver damage and protect liver cells.

[0152] 6. Antitumor activity (in vitro)

[0153] Determination method: MTT method was used to determine the proliferation inhibition rate of the decoction on human gastric cancer cells (SGC-7901) and human liver cancer cells (HepG2).

[0154] result:

[0155] SGC-7901 cell proliferation inhibition rate: At a concentration of 200 μg / mL, the proliferation inhibition rate of the decoction on SGC-7901 cells was 45%.

[0156] HepG2 cell proliferation inhibition rate: At a concentration of 200 μg / mL, the HepG2 cell proliferation inhibition rate of the drink was 50%.

[0157] Function: The drink has certain anti-tumor activity and can inhibit the proliferation of tumor cells.

[0158] It can be seen from the above biological activity test results that the drink rich in seabuckthorn flavonoids and Cistanche polysaccharides has a variety of significant biological activities, can provide antioxidant, immune enhancement, anti-fatigue, anti-inflammatory, liver protection and anti-tumor and other health benefits, and has broad application prospects and market value.

[0159] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. All modifications, equivalent substitutions, improvements, etc. within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing a Cistanche deserticola decoction rich in seabuckthorn flavonoids, characterized in that: The following steps are involved: S1: extracting flavonoids from seabuckthorn fruit and polysaccharides from Cistanche deserticola respectively to obtain seabuckthorn flavonoids extract and Cistanche deserticola polysaccharide extract; S2: Mix the seabuckthorn flavonoids extract, the Cistanche deserticola polysaccharide extract, purified water, a sweetener and an acidity regulator in a mass fraction ratio of 20%-30%: 10%-20%: 50%-60%: 2%-5%: 0.1%-0.5%, and adjust the pH value of the mixture to 5.5-6.5; S3: sterilize the mixed solution obtained in S2 to obtain a drinkable solution.

2. The method for preparing a Cistanche deserticola decoction rich in seabuckthorn flavonoids according to claim 1, characterized in that: The preparation method of the seabuckthorn flavonoids extract in step S1 is as follows: after the seabuckthorn fruit is crushed, reflux extraction is performed at 50-60° C. for 2 hours using 70% ethanol solution at a liquid-to-solid ratio of 10:1, the extract is filtered, concentrated and dried to obtain a powdered seabuckthorn flavonoids extract.

3. The method for preparing a Cistanche deserticola decoction rich in seabuckthorn flavonoids according to claim 1, characterized in that: The preparation method of the Cistanche polysaccharide extract in step S1 is as follows: crush the Cistanche, soak it in 80-90°C water for 3 hours at a liquid-to-solid ratio of 20:1 to obtain an extract, filter the extract, concentrate and dry it to obtain a powdered Cistanche polysaccharide extract.

4. The method for preparing a Cistanche deserticola decoction rich in seabuckthorn flavonoids according to claim 1, characterized in that: In step S3, the mixed solution is pasteurized at 85° C. for 15 minutes, and immediately cooled to room temperature after the pasteurization.

5. The method for preparing a Cistanche deserticola decoction rich in seabuckthorn flavonoids according to claim 1, characterized in that: The sweetener is honey or xylitol or a mixture of the two.

6. The method for preparing a Cistanche deserticola decoction rich in seabuckthorn flavonoids according to claim 1, characterized in that: The acidity regulator is citric acid.

7. The method for preparing a Cistanche deserticola decoction rich in seabuckthorn flavonoids according to claim 1, characterized in that: The method further comprises step S4: filling and sealing the drinking liquid in a sterile environment.