Nutritional composition for preventing and improving senile osteoporosis and preparation method thereof

Abstract

The invention discloses a nutritional composition for preventing and improving senile osteoporosis and a preparation method thereof, and relates to the field of food / health care products. The nutritional composition for preventing and improving senile osteoporosis is prepared from the following components in parts by weight: 400 to 600 parts of defatted roe hydrolysate, 200 to 400 parts of nano bone meal, 200 to 300 parts of sargassum fusiforme extract, 290 to 340 parts of marine collagen peptide, 60 to 80 parts of calcium citrate malate, 35 to 50 parts of N-acetylglucosamine, 10 to 20 parts of sodium hyaluronate and 5 to 10 parts of mogroside. The nutritional composition for preventing and improving senile osteoporosis provided by the invention specifically formulates a nutritional and healthy food formula helpful for preventing and improving senile osteoporosis, and multielement nutrients are matched to synergistically play a regulating role, supplement calcium and protein nutrients, improve the bioavailability of calcium, promote bone formation, prevent and improve osteoporosis and improve osteoporosis. Bone joints are repaired, the intestinal function is adjusted, and the immunity is enhanced.

Description

Technical Field

[0001] The present invention belongs to the field of food / health products, and in particular relates to a nutritional composition and a preparation method thereof which are helpful in preventing and improving osteoporosis in the elderly. Background Art

[0002] Osteoporosis is a systemic bone disease characterized by decreased bone mass and damage to bone tissue microstructure, which leads to increased bone brittleness and susceptibility to fractures. Based on the cause, osteoporosis is divided into two categories: primary osteoporosis and secondary osteoporosis. Among them, primary osteoporosis includes postmenopausal osteoporosis (type I), senile osteoporosis (type II) and idiopathic osteoporosis (including juvenile type). Secondary osteoporosis refers to osteoporosis caused by any disease and / or drug that affects bone metabolism and other clear causes.

[0003] With the increasing aging of the population in my country, osteoporosis in the elderly has become one of the chronic diseases that seriously affects the health of the middle-aged and elderly population. How to prevent osteoporosis in the elderly, delay the progression of osteoporosis in the elderly, and reduce the risk of fractures in patients has important research value and social significance. The prevention of osteoporosis is more effective than treatment, but existing therapeutic drugs generally have certain side effects. For example, the patent with publication number CN102670627B uses drugs such as bisphosphonates, which have certain side effects when taken for a long time and cannot be taken for a long time when used as a preventive measure. Therefore, the development of safe and efficient nutritional and health products that can prevent and improve osteoporosis has become an important research direction, with broad market prospects. Summary of the invention

[0004] The purpose of the present invention is to provide a nutritional composition for preventing and improving osteoporosis in the elderly, and another purpose is to provide a method for preparing the nutritional composition for preventing and improving osteoporosis in the elderly. By systematically analyzing the nutritional needs of the elderly osteoporotic population, a nutritional and healthy food formula that helps prevent and improve osteoporosis in the elderly is formulated in a targeted manner, and a multi-nutrient combination is used to synergistically exert a regulatory effect, improve the calcium bioavailability of calcium preparations, enhance immunity, supplement calcium and protein nutrition, promote bone formation, and improve osteoporosis.

[0005] To achieve the above object, the present invention provides the following technical solutions: A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 400-600 parts of defatted fish roe hydrolysate, 200-400 parts of nano bone powder, 200-300 parts of Sargassum fusiformis extract, and 340 parts of marine collagen peptide.

[0006] The nutritional composition for preventing and improving osteoporosis in the elderly also includes 60-80 parts of calcium citrate malate, 38-50 parts of N-acetylglucosamine, 10-20 parts of sodium hyaluronate, and 5-10 parts of mogroside.

[0007] Preferably, the nutritional composition for preventing and improving osteoporosis in the elderly comprises the following components, by weight: 600 parts of defatted fish roe hydrolysate, 400 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptide, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside.

[0008] The defatted fish roe hydrolysate is derived from tuna roe, and the defatted fish roe hydrolysate with a molecular weight below 3000 Da accounts for more than 70%.

[0009] The nano bone powder is derived from fish bones such as tuna and cod, and has an average particle size of 100-400nm.

[0010] The polysaccharide content in the Sargassum fusiformis extract is ≥50%.

[0011] In the marine collagen peptide, oligopeptides with a molecular weight of less than 1000 Da account for more than 60% of the total mass.

[0012] The molecular weight range of the sodium hyaluronate is 8.0×10 4 -1.0×10 5 Da.

[0013] Defatted fish roe hydrolysate is rich in essential amino acids for the human body, such as glutamic acid, lysine, proline, arginine, serine, leucine, alanine, aspartic acid, etc., which can effectively supplement protein nutrition; it has significant calcium binding capacity, which reaches 24.12 mg / g, and can effectively prevent calcium ions in calcium preparations from being precipitated by dietary factors such as oxalic acid, phytic acid, and phosphoric acid during digestion, thereby ensuring the soluble calcium content in the intestine; at the same time, it can improve the absorption and transport effect of calcium ions by small intestinal epithelial cells, and effectively improve the calcium bioavailability of calcium preparations.

[0014] The average particle size of nano bone powder is 100-400nm. The powder has a good taste and no toothache. It is rich in nutrients such as protein, calcium, and selenium. The protein content is 22-27%, and the calcium content is 1.8×10 5 ~2.0×10 5 mg / kg, selenium content 1500~2000μg / kg. Nano bone powder can supplement the calcium needed by the human body and maintain bone health; it is rich in essential trace elements and has a high content of selenium, which has significant antioxidant and anti-inflammatory effects and helps to enhance human immunity; it is rich in collagen and can supplement human protein nutrition.

[0015] Sargassum extract is extracted from Sargassum fusiformis, with a polysaccharide content of more than 50%. On the one hand, Sargassum fusiformis extract has good immunomodulatory and anti-tumor activity, which helps to enhance the immunity of the middle-aged and elderly people and strengthen daily immune protection; on the other hand, it has good probiotic properties, which helps to regulate the intestinal flora structure and improve the intestinal health of the middle-aged and elderly people.

[0016] Marine collagen peptide is a collagen hydrolyzate extracted from marine organisms, mainly from fish and their processing by-products, such as marine fish skin, fish bones, fish scales, etc. Marine collagen peptide has multiple functions: first, it promotes osteoblast proliferation, differentiation and mineralization, which is beneficial to bone formation; second, it promotes calcium absorption and transport in small intestinal epithelial cells, which helps to improve the bioavailability of calcium preparations; third, it has good anti-inflammatory and antioxidant effects, which helps to repair the body's intestinal immune barrier.

[0017] N-acetylglucosamine helps repair damaged articular cartilage and promote the formation of new articular cartilage. It also has analgesic and anti-inflammatory effects, promotes the production of joint lubricating fluid and reduces friction.

[0018] Sodium hyaluronate can promote the formation of lubricating fluid in the joint cavity, thereby improving lubrication, protecting chondrocytes and inhibiting inflammation; when used in combination with glucosamine, it can effectively improve the body's joint function and relieve pain symptoms.

[0019] Calcium citrate malate can supplement calcium and enhance and improve the flavor of the product. Mogroside can adjust the taste and has antioxidant and immune-enhancing effects.

[0020] Defatted fish roe hydrolysate, nano bone powder, marine collagen peptides and calcium citrate malate form a unique nutritional supplement formula designed to fully support bone health. The synergistic effect of these four ingredients can not only effectively supplement the calcium and protein needed by the human body, but also promote bone formation and enhance bone density, thereby improving the symptoms of osteoporosis.

[0021] The synergistic effect of Sargassum fusiformis extract and marine collagen peptide can improve the structure of intestinal flora, regulate intestinal barrier function, and enhance the body's immunity.

[0022] N-acetylglucosamine, sodium hyaluronate and marine collagen peptide work together to repair damaged articular cartilage and promote the formation of new articular cartilage, relieve pain and inflammation, promote the production of joint lubricating fluid and reduce friction.

[0023] The product form of the nutritional composition for preventing and improving osteoporosis in the elderly is a solid beverage, which can be a powder or a granule, and is applied to foods and health products for preventing and improving osteoporosis.

[0024] The present invention also provides another technical solution: A method for preparing a nutritional composition for preventing and improving osteoporosis in the elderly, characterized in that it comprises the following steps: S1: Weigh N-acetylglucosamine, sodium hyaluronate, calcium citrate malate, and mogroside according to the formula ratio, and pre-mix them using a three-dimensional mixer, with the loading amount being 60-80% of the maximum volume; S2: according to the formula ratio, weigh the defatted fish roe hydrolyzate, nano bone meal, Sargassum fusiformis extract, marine collagen peptide and the above premix and add them into the lifting hopper mixer, the loading amount is 60-80% of the maximum volume, mix and stir, and take out the materials after sufficient mixing.

[0025] The three-dimensional mixer rotates at a speed of 3-5 r / min and mixes for 10-15 min.

[0026] The rotating speed of the lifting hopper mixer is 5-8 r / min, and the mixing and stirring is performed for 15-20 min.

[0027] Therefore, the present invention has the following beneficial effects: Targeted formulation of nutritious and healthy food formulas that help prevent and improve osteoporosis in the elderly is developed. Through the synergistic regulation of multiple nutrients, it improves osteoporosis, repairs bones and joints, regulates intestinal health, and enhances the body's immunity. The core components of defatted fish roe hydrolysate and nano bone powder are added to improve the calcium binding capacity and the absorption and transport effect of small intestinal epithelial cells on calcium ions, effectively improving the calcium bioavailability of calcium preparations; supplementing calcium and protein nutrition, promoting bone formation, and improving osteoporosis. DETAILED DESCRIPTION

[0028] The present invention will be further described below in conjunction with the embodiments.

[0029] The terms used in the embodiments of the present invention are intended to describe specific embodiments rather than to limit the scope of protection of the present invention. Without departing from the spirit and scope of the inventive concept, changes and advantages that can be imagined by those skilled in the art are included in the present invention, and the attached claims and any equivalents thereof are the scope of protection of the present invention.

[0030] Example 1 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 400 parts of defatted fish roe hydrolysate, 200 parts of nano bone powder, 200 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside.

[0031] A method for preparing a nutritional composition for preventing and improving osteoporosis in the elderly comprises the following steps: S1: Weigh 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, 80 parts of calcium citrate malate, and 10 parts of mogroside, and pre-mix them in a three-dimensional mixer with a loading amount of 60% of the maximum volume, a rotation speed of 3r / min, and mixing for 10 minutes; S2: Add 400 parts of defatted fish roe hydrolysate, 200 parts of nano bone powder, 200 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides and the above premixes into a lifting hopper mixer with a loading amount of 60% of the maximum volume, a rotation speed of 5r / min, and mixing and stirring for 15 minutes. After being fully mixed, take out the materials to obtain a nutritional composition for preventing and improving osteoporosis in the elderly.

[0032] Example 2 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 400 parts of defatted fish roe hydrolysate, 200 parts of nano bone powder, 200 parts of Sargassum fusiformis extract, 290 parts of marine collagen peptides, 60 parts of calcium citrate malate, 35 parts of N-acetylglucosamine, 10 parts of sodium hyaluronate, and 5 parts of mogroside.

[0033] A method for preparing a nutritional composition for preventing and improving osteoporosis in the elderly comprises the following steps: S1: Weigh 35 parts of N-acetylglucosamine, 10 parts of sodium hyaluronate, 60 parts of calcium citrate malate, and 5 parts of mogroside, and pre-mix them in a three-dimensional mixer with a loading amount of 60% of the maximum volume, a rotation speed of 3 r / min, and mix for 10 minutes; S2: Add 400 parts of defatted fish roe hydrolysate, 200 parts of nano bone powder, 200 parts of Sargassum fusiformis extract, 290 parts of marine collagen peptide and the above premixes into a lifting hopper mixer, with the loading amount being 60% of the maximum volume, the rotation speed being 5 r / min, and the mixing and stirring for 15 minutes. After being fully mixed, the materials are taken out to obtain a nutritional composition for preventing and improving osteoporosis in the elderly.

[0034] Example 3 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 400 parts of defatted fish roe hydrolysate, 400 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside.

[0035] A method for preparing a nutritional composition for preventing and improving osteoporosis in the elderly comprises the following steps: S1: Weigh 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, 80 parts of calcium citrate malate, and 10 parts of mogroside, and pre-mix them in a three-dimensional mixer with a loading amount of 60% of the maximum volume, a rotation speed of 3r / min, and mixing for 10 minutes; S2: Add 400 parts of defatted fish roe hydrolysate, 400 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides and the above premixes into a lifting hopper mixer with a loading amount of 60% of the maximum volume, a rotation speed of 5r / min, and mixing and stirring for 15 minutes. After being fully mixed, take out the materials to obtain a nutritional composition for preventing and improving osteoporosis in the elderly.

[0036] Example 4 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 400 parts of defatted fish roe hydrolysate, 200 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside.

[0037] A method for preparing a nutritional composition for preventing and improving osteoporosis in the elderly comprises the following steps: S1: Weigh 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, 80 parts of calcium citrate malate, and 10 parts of mogroside, and pre-mix them in a three-dimensional mixer with a loading amount of 60% of the maximum volume, a rotation speed of 3r / min, and mixing for 10 minutes; S2: Add 400 parts of defatted fish roe hydrolysate, 200 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides and the above premixes into a lifting hopper mixer with a loading amount of 60% of the maximum volume, a rotation speed of 5r / min, and mixing and stirring for 15 minutes. After being fully mixed, take out the materials to obtain a nutritional composition for preventing and improving osteoporosis in the elderly.

[0038] Example 5 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 600 parts of defatted fish roe hydrolysate, 200 parts of nano bone powder, 200 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside.

[0039] A method for preparing a nutritional composition for preventing and improving osteoporosis in the elderly comprises the following steps: S1: Weigh 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, 80 parts of calcium citrate malate, and 10 parts of mogroside, and pre-mix them in a three-dimensional mixer with a loading amount of 60% of the maximum volume, a rotation speed of 3r / min, and mixing for 10 minutes; S2: Add 600 parts of defatted fish roe hydrolysate, 200 parts of nano bone powder, 200 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides and the above premixes into a lifting hopper mixer with a loading amount of 60% of the maximum volume, a rotation speed of 5r / min, and mixing and stirring for 15 minutes. After being fully mixed, take out the materials to obtain a nutritional composition for preventing and improving osteoporosis in the elderly.

[0040] Example 6 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 600 parts of defatted fish roe hydrolysate, 200 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside.

[0041] A method for preparing a nutritional composition for preventing and improving osteoporosis in the elderly comprises the following steps: S1: Weigh 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, 80 parts of calcium citrate malate, and 10 parts of mogroside, and pre-mix them in a three-dimensional mixer with a loading amount of 60-80% of the maximum volume, a rotation speed of 3 / min, and mix for 10 minutes; S2: Add 600 parts of defatted fish roe hydrolyzate, 200 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptide and the above premixes into a lifting hopper mixer, with the loading amount being 60% of the maximum volume, the rotation speed being 5 r / min, and the mixing and stirring for 15 minutes. After being fully mixed, the materials are taken out to obtain a nutritional composition for preventing and improving osteoporosis in the elderly.

[0042] Example 7 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 600 parts of defatted fish roe hydrolysate, 400 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside.

[0043] A method for preparing a nutritional composition for preventing and improving osteoporosis in the elderly comprises the following steps: S1: Weigh 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, 80 parts of calcium citrate malate, and 10 parts of mogroside, and pre-mix them in a three-dimensional mixer with a loading amount of 60% of the maximum volume, a rotation speed of 3r / min, and mixing for 10 minutes; S2: Add 600 parts of defatted fish roe hydrolysate, 400 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides and the above premixes into a lifting hopper mixer with a loading amount of 60% of the maximum volume, a rotation speed of 5r / min, and mixing and stirring for 15 minutes. After being fully mixed, take out the materials to obtain a nutritional composition for preventing and improving osteoporosis in the elderly.

[0044] Comparative Example 1 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 400 parts of nano bone powder, 200 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside. The specific implementation of this comparative example is the same as that of Example 1, except that the nutritional composition does not contain defatted fish roe hydrolysate.

[0045] Comparative Example 2 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 600 parts of defatted fish roe hydrolysate, 200 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptides, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside. The specific implementation of this comparative example is the same as that of Example 1, except that the nutritional composition does not contain nano bone powder.

[0046] Comparative Example 3 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 600 parts of defatted fish egg hydrolysate, 400 parts of nano bone powder, 200 parts of Sargassum fusiformis extract, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside. The specific implementation of this comparative example is the same as that of Example 1, except that the nutritional composition does not contain marine collagen peptide.

[0047] Comparative Example 4 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 400 parts of nano bone powder, 200 parts of Sargassum fusiformis extract, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside. The specific implementation of this embodiment is the same as that of Example 1, except that the nutritional composition does not contain defatted fish roe hydrolyzate and marine collagen peptide.

[0048] Comparative Example 5 A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 200 parts of Sargassum fusiformis extract, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate, and 10 parts of mogroside. The specific implementation of this embodiment is the same as that of Example 1, except that the nutritional composition does not contain defatted fish roe hydrolyzate, nano bone powder, and marine collagen peptide.

[0049] Table 1 Components in the compositions of Examples and Comparative Examples.

[0050] Effect test 1. Experimental Content (1) Experimental animals

[0048] As one of the experimental mouse strains, C57BL / 6 mice are widely used in the study of osteoporosis in the elderly because of their genetic background that is highly similar to humans. This mouse model can be induced to develop osteoporosis characteristics by controlling diet, hormone levels or genetic factors. C57BL / 6 mice have a fast bone remodeling ability, which makes it a valuable tool for evaluating the effectiveness of osteoporosis treatment strategies and studying bone tissue repair.

[0051] In the study of osteoporosis, the C57BL / 6 mouse model is not only suitable for mechanism research, but also very suitable for drug screening and evaluation of the efficacy of anti-osteoporosis drugs. For example, the C57BL / 6 mouse osteoporosis model established by ovariectomy has been shown to be an effective model for studying postmenopausal osteoporosis, suitable for preclinical research and pharmacokinetic evaluation. In addition, the changes in bone structure and quality of C57BL / 6 mice with age are very similar to those in the human aging process, which further verifies its applicability as a model for studying age-related bone loss in humans.

[0052] (2) Experimental methods A low-calcium diet was used to induce a calcium deficiency model in mice to evaluate the effects of different embodiments in preventing and improving osteoporosis.

[0053] Before the experiment, C57BL / 6 mice were fed for one week to ensure that they were adapted to the experimental environment and in a healthy state. Subsequently, the mice were randomly divided into 14 groups, including a normal group, a model group, example groups 1-7, and comparative example groups 1-5, with 10 mice in each group.

[0054] The mice in the normal control group were fed with a standard feed with a calcium content of 0.5% to maintain a normal calcium intake level. The mice in the model control group, the embodiment group and the comparative group were fed with a low-calcium feed with a calcium content of only 0.1% to induce a calcium deficiency state and simulate the pathological process of osteoporosis. During the experiment, the mice in the normal group and the model group were gavaged with sterile water every day, while the mice in the embodiment group and the comparative group were gavaged with the corresponding samples respectively. The dose of all gavages was unified to 1500 mg / kg to ensure the consistency of the dose. The experiment lasted for 8 weeks to observe the effects of different treatments on the bone quality of mice.

[0055] Before sampling, the mice were fasted for 12 hours to empty the gastrointestinal contents and reduce experimental errors. Subsequently, the mice were anesthetized and blood samples were collected by cardiac bleeding. After blood collection, the mice were killed by cervical dislocation. Next, the left femur of the mouse was isolated, and the attached muscles and soft tissues were carefully removed. To maintain the integrity and activity of the samples, the femur samples were wrapped in medical gauze soaked in saline and immediately stored in an ultra-low temperature freezer at -80°C. These samples will be used for subsequent bone density and bone calcium content measurements to evaluate the effects of experimental treatments on the bone health of mice.

[0056] 2. Impact on bone metabolism related indicators The levels of calcium, bone specific alkaline phosphatase (BALP), osteocalcin (OCN) in serum and the content of bone calcium in femur were detected.

[0057] Changes in serum calcium levels can reflect the state of bone metabolism and are one of the important indicators for assessing osteoporosis.

[0058] Bone-specific Alkaline Phosphatase (B-ALP) is mainly expressed in active osteoblasts and is one of the phenotypic markers of osteoblasts. It can directly reflect the activity or functional status of osteoblasts, such as the rate of bone formation and the effect of bone repair. The increase in B-ALP values ​​may indicate that the experimental composition is promoting bone formation and helping to restore the balance of bone remodeling.

[0059] Osteocalcin is a direct marker of bone formation because it is primarily secreted into the bone matrix by mature osteoblasts. Therefore, elevated osteocalcin levels are generally associated with increased bone formation activity. Elevated osteocalcin values ​​indicate increased osteoblast activity and an accelerated rate of bone formation.

[0060] The test results are shown in Table 2.

[0061] Table 2 Effects of different groups on bone metabolism-related indicators in mice As shown in Table 2, compared with the normal group, the serum calcium content of the model group was significantly reduced, and the serum calcium content in the embodiment group and the comparative example group was increased compared with the model group. The effect of the embodiment group was better than that of the comparative example, and the effect of the embodiment 7 group was better than that of the normal group; the bone calcium content of the model group was significantly lower than that of the normal group, and the bone calcium content of the embodiment group and the comparative example group was increased, among which the effects of the embodiment 3 group and the embodiment 7 group were significantly better than the normal group, and the effect of the embodiment 7 group was the best, indicating that the nutritional composition for preventing and improving osteoporosis in the elderly has the best effect when the components are matched in the optimal ratio. In summary, the nutritional composition for preventing and improving osteoporosis in the elderly can effectively promote calcium absorption in the body and help prevent osteoporosis.

[0062] Compared with the normal group, the bone formation-related markers BALP and OCN in the model group were significantly reduced, and the effects of the embodiment group and the control group were higher than those of the model group; and embodiments 1-7 were better than the normal group, indicating that the addition of the nutritional composition for preventing and improving osteoporosis in the elderly has a significant effect on regulating bone metabolism and improving bone formation in calcium-deficient mice.

[0063] 3. Impact on bone density The effects of the normal group, model group, example group and comparative group on the bone density of mice are shown in the following table.

[0064] Table 3 Effects of different groups on bone density of mice. Group Bone density Normal group 0.43 Model Group 0.36 Example 1 Group 0.43 Example 2 Group 0.44 Example 3 Group 0.45 Example 4 Group 0.44 Example 5 Group 0.45 Example 6 Group 0.44 Example 7 Group 0.47 Comparative Example 1 0.40 Comparative Example 2 0.41 Comparative Example 3 0.42 Comparative Example 4 0.39 Comparative Example 5 0.38

[0065] The bone density of mice in each group is shown in Table 3. Compared with the normal group, the bone density of the model group was significantly reduced; the bone density of mice in the embodiment group and the comparative example group was improved compared with the model group; the effect of the embodiment group was better than that of the comparative example group, the bone density of the embodiment 2-7 groups was higher than that of the normal group, and the effect of the embodiment 7 group was the best, indicating that the embodiment group had a significant effect in preventing and improving osteoporosis. This shows that in the nutritional composition for preventing and improving osteoporosis in the elderly, the components work synergistically to improve the calcium bioavailability of the calcium preparation; supplement calcium and protein nutrition, promote bone formation, and improve osteoporosis.

[0066] Therefore, the present invention adopts the above-mentioned nutritional composition and preparation method for preventing and improving osteoporosis in the elderly, adds defatted fish egg hydrolyzate, nano bone powder, marine collagen peptide and other ingredients to the nutritional composition, selects the best ratio of each component, and synergistically exerts a regulatory effect, which can avoid the side effects caused by long-term use of osteoporosis prevention and treatment drugs, supplement calcium and protein nutrition, promote bone formation, bone and joint repair, and improve osteoporosis in the elderly.

Claims

1. A nutritional composition for preventing and improving osteoporosis in the elderly, comprising the following components by weight: 400-600 parts of defatted fish roe hydrolyzate, 200-400 parts of nano bone powder, 200-300 parts of Sargassum fusiformis extract, and 290-340 parts of marine collagen peptide.

2. The nutritional composition for preventing and improving osteoporosis in the elderly according to claim 1, characterized in that: It also includes 60-80 parts of calcium citrate malate, 35-50 parts of N-acetylglucosamine, 10-20 parts of sodium hyaluronate, and 5-10 parts of mogroside.

3. The nutritional composition for preventing and improving osteoporosis in the elderly according to claim 1 or 2, characterized in that: The invention comprises the following components by weight: 600 parts of defatted fish roe hydrolysate, 400 parts of nano bone powder, 300 parts of Sargassum fusiformis extract, 340 parts of marine collagen peptide, 80 parts of calcium citrate malate, 50 parts of N-acetylglucosamine, 20 parts of sodium hyaluronate and 10 parts of mogroside.

4. The nutritional composition for preventing and improving osteoporosis in the elderly according to claim 1, characterized in that: The defatted fish roe hydrolysate is derived from tuna roe, and the defatted fish roe hydrolysate with a molecular weight below 3000 Da accounts for more than 70%.

5. The nutritional composition for preventing and improving osteoporosis in the elderly according to claim 1, characterized in that: The nano bone powder is derived from tuna and cod bones, and has an average particle size of 100-400nm.

6. The nutritional composition for preventing and improving osteoporosis in the elderly according to claim 1, characterized in that: The polysaccharide content in the Sargassum fusiformis extract is ≥50%.

7. The nutritional composition for preventing and improving osteoporosis in the elderly according to claim 1, characterized in that: In the marine collagen peptide, oligopeptides with a molecular weight of less than 1000 Da account for more than 60% of the total mass.

8. The nutritional composition for preventing and improving osteoporosis in the elderly according to claim 2, characterized in that: The molecular weight range of the sodium hyaluronate is 8.0×10 4 -1.0×10 5 Da.

9. The nutritional composition for preventing and improving osteoporosis in the elderly according to any one of claims 1 or 2, characterized in that: The product form of the composition is a solid beverage, which can be a powder or granule.

10. A method for preparing a nutritional composition for preventing and improving osteoporosis in the elderly, characterized in that: The following steps are involved: S1: Weigh N-acetylglucosamine, sodium hyaluronate, calcium citrate malate, and mogroside according to the formula ratio, and pre-mix them in a three-dimensional mixer with a loading amount of 60-80% of the maximum volume, a rotation speed of 3-5 r / min, and mix for 10-15 minutes; S2: according to the formula ratio, weigh the defatted fish roe hydrolyzate, nano bone meal, Sargassum fusiformis extract, marine collagen peptide and the premix prepared in S1 and add them into the lifting hopper mixer, the loading amount is 60-80% of the maximum volume, the speed is 5-8r / min, the mixing and stirring is carried out for 15-20min, and the materials are taken out after being fully mixed.

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