A hyaluronic acid nanogel containing rice fermentation filtrate and its preparation method and application

Abstract

The present invention relates to a hyaluronic acid nanogel containing rice fermentation filtrate and a preparation method and application thereof. The hyaluronic acid nanogel containing rice fermentation filtrate comprises hyaluronic acid nanogel and rice fermentation filtrate, and the mass ratio of the rice fermentation filtrate to the hyaluronic acid nanogel is 1:1 to 1:3. Compared with the prior art, the hyaluronic acid (HA) nanogel provided by the present invention is a new type of gel system. The hyaluronic acid (HA) nanogel used as a carrier of rice fermentation filtrate and used in coordination with a beauty instrument can significantly promote the transdermal absorption of active ingredients in the rice fermentation filtrate while keeping the epidermis intact. When rutin is used as a standard to verify the content of flavonoid components in the skin, it is found that the presence of hyaluronic acid (HA) nanogel can promote the transdermal absorption of flavonoid components, significantly improve skin gloss, reduce skin inflammation, and improve skin texture and elasticity.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemicals, and in particular relates to a hyaluronic acid nanogel containing rice fermentation filtrate, and a preparation method and application thereof. Background Art

[0002] With the improvement of the quality of life, people have more and more demands for skin improvement. Traditional beauty methods have many defects such as insufficient absorption. Under this circumstance, a large number of beauty devices have emerged. Common beauty devices are divided into microcurrent, radio frequency, ultrasonic and other types. Microcurrent beauty devices are a kind of beauty device that uses computer microwaves to interact with the human body, replenish human bioelectricity, activate cells, restore skin elasticity, and thus achieve the effect of delaying skin aging.

[0003] Chinese patent CN117959590A discloses an ion introduction system, including a power supply, the power supply is connected to a modulation module, the modulation module is electrically connected to a conductivity pulse module, the conductivity pulse module is connected to an electrode sheet, the modulation module and the electrode sheet are both connected to a controller, the controller controls the modulation module to adjust the voltage and current; the voltage and current are modulated and used by the conductivity pulse module to generate a conductivity pulse that acts on the electrode sheet; the controller is also connected to a motor, the motor drive is connected to a penetration-promoting mechanism, and the penetration-promoting mechanism promotes the rapid penetration of the drug; the controller is also connected to a collection module, the collection module is connected to a temperature sensor, and the temperature sensor monitors the working temperature; the system is provided with a human-machine interface, including a display and a touch screen control, the display displays time and drug parameters, and the touch screen sets system parameters and controls the start and stop of the system. The system is used to introduce transdermal drugs using a physical penetration-promoting method. The focus of this scheme is the design of the ion introduction system structure.

[0004] Chinese patent CN118022158A discloses a penetration control method, a beauty instrument and a storage medium. The beauty instrument includes a dot matrix heating module, an invasive temperature detection module and an ion penetration module. The method includes: controlling the dot matrix heating module to output dot matrix heat to the skin covered with the substance to be penetrated; controlling the invasive temperature detection module to detect the current skin temperature in the deep layer of the skin in real time; when the current skin temperature reaches the first preset temperature threshold, controlling the ion penetration module to output ion electroosmosis current to the skin to drive the substance to be penetrated into the skin. The dot matrix heating is used to specifically improve the permeability of the skin pores, and the ion penetration is used to promote the beauty liquid to enter the skin, thereby increasing the penetration of the beauty liquid. At the same time, the invasive temperature detection module is used to detect the temperature of the deep layer of the skin in real time, so that the dot matrix heating skin temperature is within an efficient and safe range, and while improving the penetration of the beauty liquid into the skin, the safety of the skin is ensured from being burned by heat. The focus of this solution is on the design of the beauty instrument structure.

[0005] At present, beauty devices are generally used in conjunction with some skin care products, such as essence, eye cream, skin cream, skin care lotion, etc. However, most of these conventional skin care products are directly applied to the skin surface, and then the massage effect of the beauty device is used to make the skin absorb these skin care products. However, it is difficult for skin care products to really enter the skin to exert their effects, especially since beauty liquids are easy to evaporate, and very few can enter the deep layers of the skin.

[0006] Although it is easier for beauty liquids to enter the skin through beauty devices, the effects of many beauty products on the skin remain on the surface and cannot penetrate into the skin to exert their effects. In particular, beauty liquids are volatile, and only a few can penetrate deep into the skin. Summary of the invention

[0007] Based on the problem that the beauty liquid in the prior art cannot penetrate into the deep layer of the skin to exert its efficacy, the present invention provides a hyaluronic acid nanogel containing rice fermentation filtrate and a preparation method and application thereof.

[0008] The purpose of the present invention can be achieved by the following technical solutions:

[0009] The invention firstly provides a hyaluronic acid nanogel containing rice fermentation filtrate, which comprises hyaluronic acid (HA) nanogel and rice fermentation filtrate, wherein the mass ratio of the rice fermentation filtrate to the hyaluronic acid nanogel is 1:1-1:3.

[0010] In one embodiment of the present invention, the rice fermentation filtrate is a relatively common additive in the field of cosmetics. It should be noted that the rice fermentation filtrate can be purchased commercially or prepared by itself. As a non-limiting example, yeast saccharomyces veronae can be used to prepare rice fermentation liquid through fermentation and enrichment processes, wherein Alpha-KG is rich in the rice fermentation filtrate, and the mass proportion of Alpha-KG in the rice fermentation filtrate is 400ppm-1000ppm. The preparation method of rice fermentation filtrate comprises:

[0011] Step a. Add water to rice powder, heat and stir to form 30% rice milk.

[0012] Step b. adding β-glucanase to rice milk, wherein the amount of the enzyme added is 0.001% to 0.5% of the mass of the rice milk and the enzyme activity is 150 to 200 U / g, and performing enzymolysis to obtain rice milk enzymolyzate.

[0013] Step c. inoculating the yeast strain into the rice milk enzymatic hydrolysate, and fermenting under the following process conditions: fermentation temperature of 20 to 40° C., fermentation time of 24 to 48 hours, pH value of the fermentation process controlled between 6 and 6.5, and obtaining rice fermentation liquid after fermentation.

[0014] Step d. The fermented rice fermentation liquid is concentrated by reverse osmosis technology to efficiently enrich the active substances, and the concentrated rice fermentation filtrate is obtained by heating, sterilizing, cooling and filtering, i.e., the rice fermentation filtrate containing the active substances.

[0015] Fermentation technology has a long history and is widely used in food, biomedicine and other fields. With the development of modern biotechnology, fermentation technology is used to enrich the active ingredients such as moisturizing, anti-oxidation and anti-aging in raw materials, while reducing the toxic side effects of raw materials and improving the efficacy and safety of cosmetics. Rice fermentation filtrate is a natural rice bran fermentation product, rich in γ-oryzanol, biopolysaccharides, amino acids and other active ingredients, with the effects of activating glutathione reductase, whitening, inhibiting melanin growth, inhibiting tyrosinase activity, promoting the production of cholesterol, ceramide and sebum, improving the softness of the skin, and having antioxidant, conditioning, skin rejuvenation and anti-aging effects.

[0016] In one embodiment of the present invention, the preparation method of the hyaluronic acid nanogel is:

[0017] Step 1: Modify the side chain of hyaluronic acid (HA) with methacrylic anhydride to obtain methacrylylated hyaluronic acid (HAMA);

[0018] Step 2: Methacrylylated hyaluronic acid is cross-linked with initiator diethylene glycol diacrylate (DEGDA) to obtain methacrylylated hyaluronic acid nanoparticles (HAMA nanoparticles), referred to as hyaluronic acid nanoparticles.

[0019] In one embodiment of the present invention, in step 1, the reaction of modifying the side chain of hyaluronic acid (HA) with methacrylic anhydride is carried out in an alkaline solution at -10 to 20° C. for 10 to 24 hours. Preferably, the alkaline solution is a NaOH solution.

[0020] In one embodiment of the present invention, in step 1, the amount of methacrylic anhydride and hyaluronic acid (HA) is in a molar ratio of (1-6):1.

[0021] In one embodiment of the present invention, in step 2, the molar ratio of the methacrylylated hyaluronic acid to the initiator diethylene glycol diacrylate is 1:(1-3).

[0022] In one embodiment of the present invention, in step 2, the cross-linking reaction of the methacryloyl hyaluronic acid and the initiator diethylene glycol diacrylate (DEGDA) is carried out in the presence of K2S2O8. K2S2O8 is potassium persulfate, which acts to initiate the polymerization reaction, and its amount is 0.1% to 5% of the mass of the methacryloyl hyaluronic acid, preferably 0.5%.

[0023] In one embodiment of the present invention, in step 2, the cross-linking reaction conditions of methacrylylated hyaluronic acid and initiator diethylene glycol diacrylate (DEGDA) are 70° C. and 30 min.

[0024] In one embodiment of the present invention, after the reaction in step 2, the hyaluronic acid nanogel is freeze-dried to obtain hyaluronic acid nanogel freeze-dried powder.

[0025] The preparation reaction process of the hyaluronic acid nanogel is as follows:

[0026]

[0027] The hydrogen atoms of the methylene (a, b) and methyl (c) groups are located at 6.1, 5.6 and 1.8 ppm, respectively (Ppm is parts per million, and the hydrogen atom located at 6.1 ppm means that the chemical shift of the hydrogen atom is 6.1 ppm, which is used to locate the position of the hydrogen atom and identify the chemical structure), indicating that methacrylic acid has been successfully modified on hyaluronic acid. The particle size of the nanohydrogel was determined by scanning electron microscopy and DLS analysis methods, such as Figure 1 As shown, the diameter measured by scanning electron microscopy is about 100nm and has a negative charge.

[0028] The present invention further provides a method for preparing a hyaluronic acid nanogel containing rice fermentation filtrate, which is specifically:

[0029] The freeze-dried powder of hyaluronic acid nanogel is mixed with rice fermentation filtrate to obtain hyaluronic acid nanogel containing rice fermentation filtrate, and the rice fermentation filtrate is kept in equilibrium between the water phase and the nanogel.

[0030] In one embodiment of the present invention, the concentration of the rice fermentation filtrate is 5-10 mg / ml, and the amount of the freeze-dried powder of the hyaluronic acid nanogel added to each milliliter of the rice fermentation filtrate is 5-10 mg.

[0031] In one embodiment of the present invention, mixing the freeze-dried powder of hyaluronic acid nanogel with the rice fermentation filtrate refers to stirring at room temperature overnight, the purpose of which is to fully mix and keep the rice fermentation filtrate in equilibrium between the aqueous phase and the nanogel.

[0032] The present invention further provides an application of a hyaluronic acid nanogel containing rice fermentation filtrate, wherein the hyaluronic acid nanogel containing rice fermentation filtrate is used as an introduction liquid for use with an electro-introduction beauty instrument.

[0033] Ion introduction is a method of introducing specific ions into the deep layers of the skin using electrochemical technology, usually through a special ion introduction device. Hydrogel is used as a medium for ion transfer and drug introduction. Nanogel is a new gel system. Hyaluronic acid (HA) nanogel is a good nanocarrier due to its biocompatibility, biodegradability and high encapsulation ability. The nanometer diameter of HA nanogel makes it have good permeability and can penetrate the stratum corneum of the skin. The rice fermentation filtrate is wrapped inside, which can improve its bioavailability.

[0034] The hyaluronic acid (HA) nanogel provided by the present invention is a novel gel system. The hyaluronic acid (HA) nanogel is used as a carrier of rice fermentation filtrate and in a coordinated manner with a beauty instrument to significantly promote the transdermal absorption of active ingredients in the rice fermentation filtrate while keeping the epidermis intact. When rutin is used as a standard to verify the content of flavonoid components in the skin, it is found that the presence of the hyaluronic acid (HA) nanogel can promote the transdermal absorption of flavonoid components, significantly improve skin gloss, reduce skin inflammation, and improve skin texture and elasticity.

[0035] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0036] 1. The rice fermentation filtrate and the HA nanogel carrier provided by the present invention are used in combination to achieve synergistic effect, so that the permeability of the rice fermentation filtrate is significantly improved while keeping the epidermis intact.

[0037] 2. The hyaluronic acid nanogel containing rice fermentation filtrate provided by the present invention and the electro-introduction beauty instrument can significantly promote its transdermal absorption, which is safe and harmless.

[0038] 3. The hyaluronic acid nanogel containing rice fermentation filtrate provided by the present invention and the electro-introduction beauty instrument can significantly increase the moisture content of the skin and brighten the skin tone, with significant effects and easy operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is the particle size distribution diagram of the nano hydrogel prepared in Example 1.

[0040] Figure 2 This is the Ki67 staining image of skin tissue in Example 2;

[0041] Figure 3 This is a Ki67 staining vitality image of skin tissue;

[0042] Figure 4is the standard curve of rutin;

[0043] Figure 5 is the skin moisture content at different times;

[0044] Figure 6 Skin color ITA° value;

[0045] Figure 7 Skin color value L* value;

[0046] Figure 8 Skin color value b* value. DETAILED DESCRIPTION

[0047] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0048] Example 1

[0049] This embodiment provides a hyaluronic acid nanogel containing rice fermentation filtrate, including hyaluronic acid (HA) nanogel and rice fermentation filtrate, wherein the mass ratio of the rice fermentation filtrate to the hyaluronic acid nanogel is 1:1 to 1:3.

[0050] In the present embodiment, the preparation method of rice fermentation filtrate comprises:

[0051] Step a. Add water to rice powder, heat and stir to form 30% rice milk.

[0052] Step b. adding β-glucanase to the rice milk, wherein the amount of the enzyme added is 0.1% of the mass of the rice milk and the enzyme activity is 200 U / g, and performing enzymolysis to obtain a rice milk enzymolyzate.

[0053] Step c. inoculating the yeast strain into the rice milk enzymatic hydrolysate, and fermenting under the following process conditions: fermentation temperature of 30° C., fermentation time of 36 hours, pH value of the fermentation process controlled between 6 and 6.5, and obtaining rice fermentation liquid after fermentation.

[0054] Step d. The fermented rice fermentation liquid is concentrated by reverse osmosis technology to efficiently enrich the active substances, and the concentrated rice fermentation filtrate is obtained by heating, sterilizing, cooling and filtering, i.e., the rice fermentation filtrate containing the active substances.

[0055] In this embodiment, the preparation method of hyaluronic acid nanogel is:

[0056] Step 1: Modify the side chain of hyaluronic acid (HA) with methacrylic anhydride to obtain methacrylylated hyaluronic acid (HAMA);

[0057] Step 2: Methacrylylated hyaluronic acid is cross-linked with initiator diethylene glycol diacrylate (DEGDA) to obtain methacrylylated hyaluronic acid nanoparticles (HAMA nanoparticles), referred to as hyaluronic acid nanoparticles.

[0058] Wherein, in step 1, the reaction of modifying the side chain of hyaluronic acid (HA) with methacrylic anhydride is carried out in an alkaline solution, and the reaction conditions are 0°C and 24 hours. The alkaline solution is a NaOH solution. In step 1, the amount of methacrylic anhydride and hyaluronic acid (HA) is in a molar ratio of (1 to 6):1.

[0059] In step 2, the molar ratio of the methacryloyl hyaluronic acid to the initiator diethylene glycol diacrylate is 1:(1-3). In step 2, the cross-linking reaction of the methacryloyl hyaluronic acid and the initiator diethylene glycol diacrylate (DEGDA) is carried out in the presence of K2S2O8. The amount of K2S2O8 is 0.1% to 5% of the mass of the methacryloyl hyaluronic acid, preferably 0.5%.

[0060] In step 2, the cross-linking reaction conditions of methacrylylated hyaluronic acid and initiator diethylene glycol diacrylate (DEGDA) are 70° C. and 30 min.

[0061] Scanning electron microscopy and DLS analysis methods determined the particle size of the nanohydrogels, such as Figure 1 As shown, the diameter measured by scanning electron microscopy is about 100nm and has a negative charge.

[0062] The preparation method of hyaluronic acid nanogel containing rice fermentation filtrate in this embodiment is specifically as follows:

[0063] The hyaluronic acid (HA) nanogel freeze-dried powder (10 mg) and rice fermentation filtrate (10 mg / ml) are co-dispersed to prepare the HA nanogel containing rice fermentation filtrate, and stirred overnight at room temperature to keep the rice fermentation filtrate in equilibrium between the water phase and the nanogel. The concentration of the rice fermentation filtrate is 5-10 mg / ml, and the amount of the hyaluronic acid nanogel freeze-dried powder added to each milliliter of the rice fermentation filtrate is 5-10 mg.

[0064] Example 2

[0065] Example 1 Study on transdermal absorption of hyaluronic acid nanogel containing rice fermentation filtrate and beauty instrument

[0066] First, verify the safety of the beauty instrument (the Mageline beauty egg beauty instrument is used in this example) using the electro-introduction penetration enhancement method for skin tissue:

[0067] (I) Fresh skin tissue was obtained and divided into experimental groups: the blank group was not treated, and the introduction group was introduced into PBS (phosphate buffered saline) using flexible electronic materials (hyaluronic acid nanogel) with a current of 0.6A for 25 minutes.

[0068] (ii) Tissues were sampled at 24h and 72h, with 3 replicates in each group.

[0069] (III) Stain the tissue for Ki67 to determine whether electroporation affects tissue viability.

[0070] The electroporation instrument was used to treat the skin for 25 minutes. After 24 and 72 hours of culture, the Ki67 staining and vitality values ​​of the skin tissue were as follows: Figure 2 and Figure 3 As shown, there is no significant difference in the vitality of skin tissue compared with the blank group, indicating that electrical introduction under this condition has no harm to tissue vitality.

[0071] Example 3

[0072] Example 1 Verification of the transdermal absorption effect of the hyaluronic acid nanogel containing rice fermentation filtrate and the beauty instrument (using the beauty instrument electro-introduction penetration enhancement method)

[0073] (I) Fresh skin tissue was obtained and divided into the following experimental groups: blank group (no treatment), smearing group (smearing 10 mg / mL rice fermentation filtrate), and introduction group (introduction of hyaluronic acid nanogel containing rice fermentation filtrate, wherein the concentration of rice fermentation filtrate was 10 mg / mL, and the concentration of lyophilized powder of hyaluronic acid nanogel was 10 mg / mL, and the introduction lasted for 25 min)

[0074] (ii) Clean the upper layer of skin tissue and remove the stratum corneum by sticking, then store at -80℃.

[0075] (III) Weigh 0.1 g of sample into a 2 mL vortex centrifuge tube, add 1 mL of methanol, and extract at 60°C for 4 h.

[0076] (iv) Take 0.5 mL of the crude extract (the sample obtained by washing the upper layer of the skin tissue and the mixture obtained after centrifugation), add 4.5 mL of methanol, and then add 0.3 mL of NaNO2 solution, and let it stand at room temperature for 5 minutes.

[0077] (v) Add 0.3 mL of AlCl3 solution and let stand for 6 min.

[0078] (vi) Add 4 mL of NaOH, add water to make up to 10 mL, and let stand for 15 min.

[0079] (VII) Measure the absorbance at 510 nm.

[0080] Using rutin as the standard, the standard curve is as follows Figure 4 As shown, Figure 4 In the figure, the horizontal axis is the concentration of rutin and the vertical axis is the absorbance value. The rutin samples are samples of different concentrations prepared by adding methanol to pure rutin. The total flavonoid content in the skin tissue of each group is shown in Table 1. Compared with the blank group, there was no significant difference in the total flavonoid content of the smear group, but the total flavonoid content of the introduction group was significantly increased by 65.08% compared with the blank group, indicating that the electro-introduction method helps to promote the transdermal absorption of rice fermentation filtrate.

[0081] Table 1 Total flavonoid content in skin tissue of each group

[0082]

[0083] Compared with the blank group **P<0.01

[0084] Example 4

[0085] Provided is a method for preparing a beauty gel containing rice fermentation filtrate, comprising the following preparation processes:

[0086] According to the above ratio and method, HA gel containing rice fermentation filtrate is prepared, and then the components in Table 2 are added in sequence, stirred evenly, and sterilized to obtain the HA gel.

[0087] Table 2 Ratio of components of the beauty gel containing rice fermentation filtrate in this embodiment

[0088]

[0089] In the beauty gel containing rice fermentation filtrate in this embodiment, the HA gel containing rice fermentation filtrate is the HA nanogel containing rice fermentation filtrate prepared in Example 1, that is, the lyophilized powder (10 mg) of hyaluronic acid (HA) nanogel and rice fermentation filtrate (10 mg / ml) are co-dispersed to prepare the HA nanogel containing rice fermentation filtrate. 1,3-Butanediol, pentanediol, and hexanediol are moisturizers, and ceramide has the effects of maintaining skin barrier, moisturizing, anti-aging, and whitening.

[0090] Further, clinical testing on subjects was supplemented.

[0091] The research object is 33 Chinese healthy sensitive skin women, aged between 25 and 45 years old, with an average age of 40.54 years old, and the subject makes written consent in the case of knowing. The subject is willing to cooperate and understand the necessity and duration of control, so as to comply with the scheme formulated by the clinical trial center. It is divided into 3 groups, 11 people in each group, respectively using the gel corresponding to the embodiment, comparative example 1, and comparative example 2 in Table 2, using once every night (using method is: after cleaning the face, taking a quantitative beauty gel and applying it gently to the face, then using a beauty instrument to massage gently for 10 minutes), using it continuously for 14 days, respectively before use, immediately, after 7 days and after 14 days (D0, T1, D7 and D14) by the experimenter using skin moisture content measuring instrument Corneometer CM825 to detect skin moisture content, skin gloss measuring instrument Glossymeter GL200 to detect skin glossiness. The data were statistically analyzed using SPSS 28.0, and the test data were tested for normal distribution. If the test data were normally distributed, the T test method was used for statistical analysis; if the test data were non-normally distributed, the rank sum test method was used for statistical analysis. The rank sum test was used for statistical analysis of graded data. The statistical method significance level was p < 0.05.

[0092] Depend on Figure 5 It can be seen that the embodiment group has a significant increase of 15.35% in the water content of the stratum corneum of the skin after using the sample immediately, 27.59% after 7 days of use, and 42.53% after 14 days of use. It shows that the gel can achieve an immediate and long-lasting moisturizing effect when used in combination with a beauty instrument. Compared with before use, the comparative example 1 group has an increase of 2.11% in the water content of the stratum corneum of the skin immediately after using the sample, 8.46% after 7 days of use, and 12.47% after 14 days of use. Compared with before use, the comparative example 2 group has an increase of 10.67% in the water content of the stratum corneum of the skin immediately after using the sample, 20.92% after 7 days of use, and 36.38% after 14 days of use. This indicates that rice fermentation filtrate has a good moisturizing effect, and HA nanogel can promote the rice fermentation filtrate to penetrate the stratum corneum and improve its bioavailability, and the two have a synergistic effect.

[0093] like Figure 6-8As shown, compared with the group before using the sample, the L* value of the subject's skin color increased by 0.52%, the b* value of the skin color decreased by 1.91%, and the ITA° value of the skin color increased by 0.83% immediately after using the sample. After using the sample for 7 days, the L* value of the subject's skin color increased by 3.28%, the b* value of the skin color decreased by 13.74%, and the ITA° value of the skin color increased by 6.11%. After using the sample for 14 days, the L* value of the subject's skin color increased by 6.03%, the b* value of the skin color decreased by 17.94%, and the ITA° value of the skin color increased by 12.78%. This shows that the hyaluronic acid nanogel containing rice fermentation filtrate combined with a beauty instrument promotes transdermal absorption and has the effect of brightening the skin color. Compared with the group before use, the L* value, b* value and ITA° value of the skin color of the subjects in the comparative example 1 group did not change immediately after using the sample. After 7 days of use, the L* value of the skin color of the subjects increased by 0.27%, the b* value decreased by 0.71%, and the ITA° value increased by 1.15%. After 14 days of use, the L* value of the skin color of the subjects increased by 0.93%, the b* value decreased by 1.42%, and the ITA° value increased by 1.72%. Compared with the group before use, the L* value of the skin color of the subjects in the comparative example 2 group increased by 0.27%, the b* value decreased by 1.11%, and the ITA° value increased by 0.28% immediately after using the sample. After 7 days of using the sample, the L* value of the skin color of the subjects increased by 1.86%, the b* value decreased by 8.89%, and the ITA° value increased by 4.82%. After 14 days of using the sample, the L* value of the skin color of the subjects increased by 4.12%, the b* value decreased by 14.44%, and the ITA° value increased by 9.92%. This indicates that rice fermentation filtrate has the effect of brightening skin tone, and HA nanogel can promote the penetration of rice fermentation filtrate through the stratum corneum and improve its bioavailability, and the two have a synergistic effect.

[0094] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A hyaluronic acid nanogel containing rice fermentation filtrate, characterized in that: The invention comprises hyaluronic acid nanogel and rice fermentation filtrate, wherein the mass ratio of the rice fermentation filtrate to the hyaluronic acid nanogel is 1:1-1:

3.

2. A hyaluronic acid nanogel containing rice fermentation filtrate according to claim 1, characterized in that, The preparation method of the hyaluronic acid nanogel is: Step 1: Modify the side chain of hyaluronic acid with methacrylic anhydride to obtain methacrylylated hyaluronic acid; Step 2: Methacrylylated hyaluronic acid is cross-linked with initiator diethylene glycol diacrylate to obtain methacrylylated hyaluronic acid nanogel, namely hyaluronic acid nanogel.

3. A hyaluronic acid nanogel containing rice fermentation filtrate according to claim 2, characterized in that, In step 1, the reaction of modifying the side chain of hyaluronic acid with methacrylic anhydride is carried out in an alkaline solution at -10 to 20° C. for 10 to 24 hours.

4. A hyaluronic acid nanogel containing rice fermentation filtrate according to claim 2, characterized in that, In step 1, the molar ratio of methacrylic anhydride to hyaluronic acid is (1-6):

1.

5. A hyaluronic acid nanogel containing rice fermentation filtrate according to claim 2, characterized in that, In step 2, the molar ratio of the methacrylylated hyaluronic acid to the initiator diethylene glycol diacrylate is 1:(1-3).

6. A hyaluronic acid nanogel containing rice fermentation filtrate according to claim 2, characterized in that: In step 2, the cross-linking reaction between the methacryloyl hyaluronic acid and the initiator diethylene glycol diacrylate is carried out in the presence of K2S2O8, and the amount of K2S2O8 is 0.1% to 5% of the mass of the methacryloyl hyaluronic acid.

7. A hyaluronic acid nanogel containing rice fermentation filtrate according to claim 2, characterized in that: After the reaction in step 2, the hyaluronic acid nanogel is freeze-dried to obtain hyaluronic acid nanogel freeze-dried powder.

8. The method for preparing the hyaluronic acid nanogel containing rice fermentation filtrate according to claim 1, characterized in that: The following steps are involved: The freeze-dried powder of hyaluronic acid nanogel is mixed with rice fermentation filtrate to obtain hyaluronic acid nanogel containing rice fermentation filtrate, and the rice fermentation filtrate is kept in equilibrium between the water phase and the nanogel.

9. The method for preparing hyaluronic acid nanogel containing rice fermentation filtrate according to claim 8, characterized in that: The concentration of the rice fermentation filtrate is 5-10 mg / ml, and the amount of the freeze-dried powder of the hyaluronic acid nanogel added to each milliliter of the rice fermentation filtrate is 5-10 mg.

10. The use of a hyaluronic acid nanogel containing rice fermentation filtrate according to claim 1, characterized in that: The hyaluronic acid nanogel containing rice fermentation filtrate is used as an introduction liquid for use with an electro-introduction beauty instrument.

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