IgY oral burst bead with antibacterial efficacy and preparation method thereof

Abstract

The present application relates to the technical field of oral care products, and particularly relates to an IgY oral explosion bead with bacteriostatic effect and a preparation method thereof. The oral explosion bead comprises a capsule skin and a core liquid, and the core liquid contains IgY and aniseed essential oil. The oral explosion bead can effectively cause Helicobacter pylori, Porphyromonas gingivalis and the like which cause oral odor, and the IgY and the aniseed essential oil are used in combination to jointly achieve a bacteriostatic effect. The IgY and the aniseed essential oil are prepared into an IgY-aniseed essential oil wrapping liquid form in the present application, and the stability and the bacteriostatic effect of the oral explosion bead are further improved. The mixing ratio and the adding ratio of the IgY and the aniseed essential oil are researched in a large amount in the present application, and the optimal formula and the preparation method are confirmed. The technical scheme can solve the technical problems that the bacteriostatic effect of the IgY in the oral explosion bead is difficult to fully exert and the stability is poor, raw materials of the formula are easy to obtain, the production method is simple, the cost is low, the effect is remarkable, and the present application is suitable for vigorous promotion.

Description

Technical Field

[0001] The present invention relates to the technical field of oral care products, and in particular to an IgY oral bursting bead with antibacterial effect and a preparation method thereof. Background Art

[0002] Yolk immunoglobulins (IgY), also known as yolk antibodies, are specific antibodies produced by laying hens after immunization with specific antigens. They are transferred and stored in the egg yolk and possess excellent antibacterial and antiviral properties. Studies have shown that specific yolk antibodies can provide effective passive immunity in humans and animals. Immunizing laying hens with bacterial vaccines can produce specific yolk antibodies against Helicobacter pylori. Oral administration of specific yolk antibodies can effectively inhibit Helicobacter pylori, freshen breath, and protect oral health. These antibodies are also safe, have no toxic side effects, and are low-cost to produce.

[0003] Oral-bursting beads are similar to micro-soft capsules, encapsulating a specific content within a wall material. They are widely used to freshen breath, eliminate bad breath, and regulate the oral environment, representing a new form of IgY application. Publication No. CN 117770458 A discloses probiotic oral-bursting beads and a preparation process, belonging to the field of probiotic encapsulation technology. The probiotic oral-bursting beads comprise a core material and a wall material. The core material includes probiotic raw materials, flavorings, mannose, xylitol, manno-oligosaccharides, glycine, vitamin E, and solvent 1, which is an oily solvent. The wall material includes trehalose, gelatin, cyclodextrin, and solvent 2, which is an oily solvent. The probiotic oral-bursting beads are heat-resistant, acid-resistant, and durable. Publication No. CN114748608A discloses an anti-oral Helicobacter pylori popping chewing gum pill, which is composed of the following substances in parts by weight: 0.1-2.5 parts of licorice; 0.01-1.0 parts of tea polyphenols; 1.0-20.0 parts of probiotics; 0.1-5.0 parts of milk protein; 0.5-8.0 parts of menthol; 0.1-0.5 parts of sweetener; 6-8 parts of gelatin; 0.5-2 parts of glycerol; and edible vegetable oil, added to 100 parts by weight. The preparation method of the aforementioned chewing gum pill is disclosed, which has the advantages of quickly eliminating bad breath, inhibiting oral HP and pathogens, and maintaining normal oral microecology.

[0004] Bacteria that may cause bad breath include Helicobacter pylori and Porphyromonas gingivalis, which can also cause gingivitis, periodontitis, and oral ulcers, affecting oral health. When used alone, IgY has a certain effect on inhibiting Helicobacter pylori, which causes bad breath, but has no significant inhibitory effect on Porphyromonas gingivalis and Candida albicans, and it is easy to precipitate when added to oral blasting beads. From the perspective of practical application, its therapeutic effect is subject to certain limitations. It can be seen that when IgY is applied to oral care products, its antibacterial effect is difficult to fully exert, and if it is prepared in the form of oral blasting beads, the compatibility of IgY with other materials also has certain problems. It is urgent to fully study the way to give full play to the antibacterial effect of IgY and explore a way to improve the compatibility of IgY with other materials. Summary of the Invention

[0005] The purpose of the present invention is to provide an IgY oral bursting bead with antibacterial efficacy to solve the technical problems that the antibacterial effect of IgY in the oral bursting bead dosage form is difficult to fully exert and the stability is poor.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0007] The invention discloses an IgY oral explosive bead with antibacterial effect, comprising a capsule and a core liquid; the core liquid contains IgY and fennel essential oil.

[0008] Furthermore, the raw materials of the core liquid include, by weight: 8-35 parts of natural menthol, 0.01-1 part of sucralose, 0.001-0.5 parts of neotame, 1-20 parts of edible flavor, 0.01-2 parts of IgY, and 0.01-2 parts of fennel essential oil; preferably, IgY is 0.01-1 part and fennel essential oil is 0.01-1 part.

[0009] Furthermore, the raw materials for the capsule shell include, by weight, 20-55 parts gelatin, 0.01-1 part sucralose, 0.1-5 parts erythritol, 2-15 parts glycerin, and 0.000001-0.0005 parts food coloring. Food coloring can be added or not as needed and is a non-essential ingredient, primarily used to adjust the appearance of the popping bead product.

[0010] Furthermore, the mass ratio of IgY to fennel essential oil is 1:1.

[0011] Furthermore, the raw materials of the core liquid and the capsule skin are dissolved in a solvent respectively; the solvent includes at least one of medium-chain triglycerides, walnut oil, coconut oil, olive oil, and vegetable oil.

[0012] Furthermore, the particle size of the IgY oral bursting beads is 4-10 mm.

[0013] Furthermore, the core liquid is prepared by the following method: in a temperature environment of 20-25°C, IgY is added while stirring fennel essential oil at a speed of 200 rpm-500 rpm, and after the addition, stirring is continued at a speed of 700 rpm-1000 rpm for 5-10 min to form an IgY-fennel essential oil coating liquid; the IgY-fennel essential oil coating liquid is mixed with the remaining raw materials and solvent of the core liquid to obtain the core liquid.

[0014] The key component of the IgY oral bursting beads with antibacterial efficacy of this technical solution lies in the core liquid, while the capsule skin of the bursting beads is the conventional soft capsule skin of the prior art. The appropriate raw material ratio setting ensures good compatibility between the core liquid of this technical solution and the capsule material containing gelatin, ensuring the overall stability of the bursting bead product. Therefore, in addition to being used directly as an oral antibacterial freshener, it can also be loaded into the capsule skin for easy transportation and use, expanding the application form of oral antibacterial fresheners.

[0015] The method for preparing IgY oral explosive beads with antibacterial efficacy comprises the following steps:

[0016] S1 core material preparation:

[0017] IgY was added while stirring the fennel essential oil at a speed of 200 rpm to 500 rpm, and after the addition, stirring was continued at a speed of 700 rpm to 1000 rpm for 5 to 10 minutes to form an IgY-fennel essential oil coating solution; the IgY-fennel essential oil coating solution was mixed with the remaining material of the core solution to obtain a mixture A;

[0018] S2 wall material preparation: Stir the capsule shell materials evenly to obtain mixture B;

[0019] S3 Dropping Pills: Mixture A and mixture B are prepared into semi-finished pellets;

[0020] S4 Cooling, drying and pill sorting: After the semi-finished oral popping beads are cooled and dried, they are screened to obtain the finished oral popping beads.

[0021] Furthermore, in S1, the preparation temperature of mixture A is 20-25°C; and in S2, the preparation temperature of mixture B is 50-80°C.

[0022] Furthermore, in S3, the pellet size is 4-10 mm.

[0023] Furthermore, in S3, the temperature of mixture B is controlled to be 65-80°C, the pump speed is controlled to be 3-10 rpm, the temperature of mixture A is controlled to be 20-65°C, the pump speed is controlled to be 20-70 rpm, and the dripping rate is controlled to be 3-18 particles / second.

[0024] Furthermore, in S2, the capsule shell material is stirred at 40-70°C; and in S4, the drying temperature is 20-30°C.

[0025] The present technical solution also provides a composition with antibacterial efficacy, comprising IgY and fennel essential oil.

[0026] The present technical solution also provides an oral care product containing a composition with antibacterial efficacy, wherein the raw materials thereof include, by weight, 8-35 parts of natural menthol, 0.01-1 part of sucralose, 0.001-0.5 parts of neotame, 1-20 parts of edible flavor, 0.01-2 parts of IgY, and 0.01-2 parts of fennel essential oil;

[0027] The raw materials are dissolved in a solvent; the solvent comprises at least one of medium chain triglycerides, walnut oil, coconut oil, olive oil, and vegetable oil;

[0028] Preferably, the amount of IgY is 0.01-1 part, and the amount of fennel essential oil is 0.01-1 part.

[0029] The present technical solution also provides a method for preparing an oral care product, comprising adding IgY while stirring fennel essential oil at a speed of 200 rpm-500 rpm, and continuing to stir at a speed of 700 rpm-1000 rpm for 5-10 min to form an IgY-fennel essential oil coating liquid; and mixing the IgY-fennel essential oil coating liquid with the remaining materials of the oral care product to obtain the product.

[0030] The present technical solution also provides an application of an oral care product having an antibacterial effect composition in the preparation of oral popping beads, wherein the oral popping beads include a capsule shell, in which the oral care product is wrapped; the raw materials of the capsule shell include: 20-55 parts of gelatin, 0.01-1 part of sucralose, 0.1-5 parts of erythritol, and 2-15 parts of glycerol.

[0031] The technical principle and beneficial effects of this technical solution are:

[0032] The present invention adds edible IgY and fennel essential oil to the formula, which has the effects of antibacterial, removing oral odor, and freshening breath. The oil-soluble fennel essential oil is premixed with the water-soluble IgY to improve the stability of IgY in the oral popping beads. The formula raw materials of the present invention are easy to obtain, the preparation method is simple, the cost is low, the effect is significant, and it is suitable for vigorous promotion. In this technical solution, fennel essential oil and IgY are compatible, and its antibacterial effect is better (Experimental Example 2), and the addition of edible oily antibacterial components to form a wrapping system with IgY may inhibit IgY precipitation and increase the stability of the oral popping beads (Experimental Example 3). The oral popping beads of this technical solution have the functions of inhibiting oral microorganisms (if anti-Helicobacter pylori IgY is used, it has the function of anti-Helicobacter pylori), freshening breath, etc. When using, it is only necessary to contain the popping beads in the mouth and bite them, and the effective core liquid part flows out to cover the oral cavity, and can be spit out after a period of time. The overall raw materials of the popping beads are edible raw materials and can also be swallowed directly. DETAILED DESCRIPTION

[0033] The present invention will be further described in detail below with reference to the examples, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the technical means used in the following examples and experimental examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used are all commercially available.

[0034] The overall situation of this technical solution is as follows:

[0035] The invention discloses an IgY oral explosive bead with antibacterial effect, comprising a capsule and a core liquid.

[0036] The capsule shell comprises the following raw materials by weight: 20-55 parts gelatin, 0.01-1 part sucralose, 0.1-5 parts erythritol, 2-15 parts glycerin, 0.000001-0.0005 parts food coloring, and solvent added to 100 parts. The food coloring is primarily used to give the capsule a certain color, making it more aesthetically pleasing. If this is not required, the food coloring can be omitted.

[0037] The core liquid comprises the following raw materials in parts by weight: 8-35 parts of natural menthol, 0.01-1 part of sucralose, 0.001-0.5 part of neotame, 1-20 parts of edible flavor, 0.01-2 parts of IgY, 0.01-2 parts of fennel essential oil, and solvent is added to 100 parts.

[0038] The solvent is at least one of medium chain triglyceride, walnut oil, coconut oil, olive oil and vegetable oil.

[0039] IgY is an egg yolk immunoglobulin, a specific egg yolk antibody with anti-Helicobacter pylori function. The IgY used in this protocol was purchased from Youlikang (Jiangsu) Biopharmaceutical Co., Ltd. This IgY product is obtained by immunizing laying hens with a complex of four antigenic proteins: urease (UreB), vacuolating cytotoxin (VacA), cytotoxin gene A (CagA), and adhesin HpaA, followed by separation and purification of the egg yolk. In practice, a variety of IgY types can be used, not limited to anti-Helicobacter pylori IgY.

[0040] Fennel essential oil is obtained by steam distillation of star anise fruit and fresh leaves of the star anise tree. It is a conventional product in the prior art. The fennel essential oil used in this solution was purchased from Guangxi Yulan Biotechnology Co., Ltd. The quality standards for fennel essential oil are detailed in the "Chinese Pharmacopoeia" (Pharmacopoeia 2020 Edition, Part I, Plant Oils and Extracts). It is a volatile oil extracted by steam distillation from fresh branches and leaves or mature fruits of the Magnoliaceae plant Illicium verum Hook.f. . The fennel essential oil used in this solution meets the above quality standards. The quality standards for fennel essential oil can also be detailed in the "National Food Safety Standard Food Additive Star Anise Oil" (GB 1886.140-2015). It is a food additive star anise oil extracted from the fruits and leaves of the star anise tree (Illicium verum Hook.f.) in southern China by steam distillation. The fennel essential oil used in this solution meets the above quality standards. That is, the fennel essential oil purchased in this solution meets the requirements of both of the above current standards. Fennel essential oil has antibacterial properties, killing bacteria in the mouth and removing bad breath. The optimal ratio of fennel essential oil to IgY is 1:1 (by mass).

[0041] Food coloring includes brilliant blue, lemon yellow, titanium dioxide, fruit green, carmine, etc.

[0042] Edible flavors include jasmine flavor, lemon flavor, mint flavor, peach flavor, cucumber flavor, oolong tea flavor, etc.

[0043] An IgY oral explosive bead with antibacterial effect is prepared by the following method:

[0044] S1 core material preparation:

[0045] a. The fennel essential oil and IgY were weighed in a mass ratio, and IgY was added while stirring the fennel essential oil at a stirring speed of 200rpm-500rpm. After the addition was complete, the stirring speed was set to 700rpm-1000rpm and stirred for 5-10mim to form an IgY- fennel essential oil package liquid.

[0046] b. The IgY- fennel essential oil package liquid and other core liquid materials used in proportion mixed and stirred, dispersed evenly, the liquid preparation temperature 20-25 ℃, to obtain the core liquid.

[0047] S2 wall material preparation:

[0048] Add the wall material raw materials into the solvent, stir evenly at 40-70℃, make it clear without bubbles, and prepare the liquid at 50-80℃ to obtain the rubber liquid.

[0049] S3 dripping pills:

[0050] Place the prepared wall material raw materials into the liquid tank and the required core material raw materials into the core liquid tank. The pellets are then dripped into the pellet machine according to the conventional production process for popping beads (pills). The rubber liquid temperature is controlled at 65-80°C, the pump speed at 3-10 rpm, the core liquid temperature at 20-65°C, the pump speed at 20-70 rpm, the dripping rate at 3-18 pellets / second, and the coolant temperature at 15°C. The coolant is paraffin oil or caprylic triglyceride. The pellets are uniform in size and smooth in surface, with a diameter of 4-10 mm. Through the pellet process, a semi-finished popping bead product is obtained.

[0051] S4 cooling, drying and pill sorting:

[0052] After the semi-finished popping beads obtained in the previous step are cooled, they are placed at a temperature of 20-30°C for drying, and the pellets are screened and selected to obtain the finished oral popping beads.

[0053] Example 1:

[0054] The following core liquid formula is used: 20 parts of natural menthol, 0.3 parts of sucralose, 0.1 parts of neotame, 20 parts of edible flavoring, 0.01 parts of IgY, 0.01 parts of fennel essential oil, and coconut oil is used to make up the balance to 100 parts.

[0055] The following wall material formula is used: 35 parts of gelatin, 0.08 parts of sucralose, 1 part of erythritol, 8 parts of glycerin, 0.00015 parts of yellow pigment, and coconut oil is used to make up the balance to 100 parts.

[0056] The preparation process is as follows:

[0057] S1 core material preparation:

[0058] a. The fennel essential oil and IgY were weighed in a mass ratio, and IgY was added while stirring the fennel essential oil at a stirring speed of 350 rpm. After the addition was complete, the stirring speed was set to 850 rpm and stirred for 8 min to form an IgY- fennel essential oil encapsulated liquid.

[0059] b. Mix the IgY-fennel essential oil package liquid and other materials used in the core liquid in proportion and stir until evenly dispersed. Control the liquid temperature at 23°C.

[0060] S2 wall material preparation:

[0061] Add the wall material raw materials into the solvent, stir evenly, make sure it is clear without bubbles, and control the liquid temperature at 75℃.

[0062] S3 dripping pills:

[0063] The temperature of the rubber liquid was controlled at 75°C, the pump speed was 7 rpm, the temperature of the core liquid was controlled at 40°C, the pump speed was controlled at 50 rpm, the dripping rate was controlled at 10 pellets / second, and the pellets were ensured to be uniform in size and smooth in surface, thereby obtaining the semi-finished product of the popping beads.

[0064] S4 cooling, drying and pill sorting:

[0065] The pellets obtained in the previous step are cooled and then dried in a tumbler at a drying temperature of 25°C. The pellets are screened and selected to obtain the finished product of the popping pellets.

[0066] The formulas of Examples 1-4 and Comparative Examples 1-10 are detailed in Tables 1 and 2, all of which are used to prepare the IgY oral bursting beads with antibacterial efficacy of this solution. The other Examples and Comparative Examples were prepared according to the preparation process of Example 1. For the comparative examples lacking IgY and fennel essential oil, it is only necessary not to add the substances in the corresponding operation steps. In addition, Comparative Example 11 is basically the same as Example 3, except that an equal amount of lemon essential oil is used instead of fennel essential oil. Lemon essential oil is an essential oil obtained by conventional steam distillation, and all other aspects are consistent with Example 3.

[0067] Table 1: Composition of the formula of the embodiment

[0068]

[0069]

[0070] Table 2: Comparative Example Formula Composition

[0071]

[0072] Experimental Example 1: IgY activity test

[0073] The titer of IgY in the oral explosive beads of the embodiment and the comparative example was determined by ELISA test method. The general operation steps are as follows:

[0074] (1) Use pH 9.6 carbonate buffer or PBS buffer to dilute UreB (2 μg / ml), CagA (0.5 μg / ml), VacA (0.5 μg / ml) and HpaA (1 μg / ml), add the diluted antigens to the wells of the ELISA plate (100 μl / well), apply sealing film, and incubate at 4°C overnight.

[0075] (2) Discard the coating solution and wash three times with TBST containing 0.05% Tween 20.

[0076] (3) Add 300 μl of TBS blocking solution containing 3% BSA to each well, incubate at 37°C for 1 h, and wash three times with TBST.

[0077] (4) The above oral explosive bead solution (contents) was thoroughly shaken and mixed, and the samples were serially diluted starting from 1:100 (see Table 3 for details). The samples were added to the ELISA plate at a volume of 100 μl / well, incubated at 37°C for 40 minutes, and washed five times with PBST.

[0078] (5) Dilute HRP-goat anti-chicken IgY antibody 1:2000 with enzyme diluent, 100 μl / well, incubate at 37°C for 40 min, and wash 5 times with PBST.

[0079] (6) Add 100 μl / well of TMB colorimetric solution and incubate at room temperature for 5-15 min. Terminate the colorimetric reaction with 2N sulfuric acid and read the result on a microplate reader. The OD value at 450 nm is taken. A value greater than 1.6 times that of the negative control is considered positive. The maximum dilution factor of the positive well is the antibody titer. The experimental results are detailed in Table 4.

[0080] Table 3: Sample dilution gradient

[0081] serial number HpaA Vac Cag Ure A 1:100 1:100 1:100 1:100 B 1:200 1:200 1:200 1:200 C 1:400 1:400 1:400 1:400 D 1:800 1:800 1:800 1:800 E 1:1600 1:1600 1:1600 1:1600 F 1:3200 1:3200 1:3200 1:3200 G 1:6400 1:6400 1:6400 1:6400 H blank blank blank blank

[0082] Table 4: Titer test results

[0083]

[0084]

[0085] The above experimental results show that oral blasting solution without IgY showed no binding titer to the four surface protein antigens of Helicobacter pylori. However, oral blasting solution with IgY showed a certain binding titer to all four surface protein antigens of Helicobacter pylori, and the binding titer increased to a certain extent with the increase in the added ratio of IgY.

[0086] Experimental Example 2: Antibacterial Test

[0087] Possible causes of bad breath include Helicobacter pylori infection, dental plaque, gingivitis, periodontitis, oral ulcers or dental caries, etc. These diseases may cause bacteria to grow in the mouth and produce bad breath. Helicobacter pylori is a type of microaerophilic Gram-negative helicobacter that can colonize the human gastrointestinal tract and is a pathogen that produces ammonia compounds. These compounds reflux into the oral cavity through the digestive tract to produce bad breath, oral ulcers, etc. The antibacterial ability of the oral explosive beads of the embodiments and comparative examples in Tables 1 and 2 against Helicobacter pylori was evaluated by the paper diffusion method.

[0088] The operation steps are as follows: using the disc diffusion method, with physiological saline (0.9% sodium chloride) as the negative control group, and bovine brain heart infusion broth plus agar to prepare the solid culture medium. Using a sterile cotton swab to dip the bacteria that have been incubated for 72 hours, prepare a medium containing Helicobacter pylori with a concentration of 1×10 7 CFU / mL bacterial suspension. 100 μL of the bacterial suspension was placed on a plate. After evaporation, a filter paper disc was placed in the center of the bacterial plate. 10 μL of different oral explosive bead solutions (the contents of the explosive bead, i.e., the core solution) was added dropwise until completely soaked (each sample was repeated in triplicate). Gently press with sterile tweezers and incubate at 37°C in a three-gas incubator (O2: 5%; CO2: 15%; N2: 80%) for 48 hours. The diameter of the inhibition zone (mm) of each disc was recorded. The average diameter of the inhibition zone that showed an inhibitory effect in the three replicates was calculated. The experimental results are detailed in Table 5.

[0089] The diameters of the Helicobacter pylori inhibition rings in Examples 1-4 were all above 25 mm. The antibacterial effects increased substantially with increasing amounts of IgY and fennel essential oil, significantly exceeding those in Comparative Examples 1-3, which did not contain IgY or fennel essential oil, or only contained IgY. When the amounts of IgY and fennel essential oil added exceeded 2%, the increase in antibacterial effect was insignificant. When the IgY addition levels were consistent, and fennel essential oil and IgY were added in a 1:1 ratio, the antibacterial effect was superior to that obtained without the 1:1 ratio. More specifically, in Comparative Example 4, IgY and fennel essential oil were added in a 0.2:0.1 ratio. While this ratio increased the amount of active ingredients compared to Example 2 (IgY and fennel essential oil were added in a 0.1:0.1 ratio), the antibacterial effect of the product in Comparative Example 4 was inferior to that of Example 2, as measured by the inhibition ring data. Similarly, in Comparative Example 5, IgY and fennel essential oil are added in a ratio of 1:1.5. Compared with the method of Example 3 (IgY and fennel essential oil are added in a ratio of 1:1), although more effective ingredients are added, from the antibacterial ring data, the antibacterial effect of the product of Comparative Example 5 is not as good as that of Example 3. This shows that the appropriate ratio of IgY and fennel essential oil is critical to maintaining the antibacterial effect of oral popping beads, and a ratio of 1:1 is appropriate. Too high or too low a ratio of IgY and fennel essential oil is not conducive to the synergistic effect of the two. In Comparative Example 6, the number of parts of IgY and fennel essential oil is increased to 2.2 parts, but the increase in the amount of active ingredients does not effectively bring about a very significant improvement in the antibacterial effect. Therefore, it is more appropriate to maintain the number of parts of IgY and fennel essential oil below 2 parts, which can ensure the effect and save raw materials. According to Comparative Example 11, using lemon essential oil instead of fennel essential oil does not have a significant impact on the antibacterial effect. However, subsequent experiments show that although lemon essential oil does not affect the antibacterial effect, it does not improve the stability of the entire capsule system.

[0090] Table 5: Antibacterial ring diameter test results

[0091] Group Inhibition ring (mm) Example 1 25.96±0.15 Example 2 27.93±0.21 Example 3 29.03±0.33 Example 4 29.91±0.26 Comparative Example 1 12.65±0.07 Comparative Example 2 20.23±0.24 Comparative Example 3 17.12±0.15 Comparative Example 4 25.01±0.27 Comparative Example 5 26.24±0.25 Comparative Example 6 29.94±0.34 Comparative Example 7 15.12±0.12 Comparative Example 8 23.13±0.35 Comparative Example 9 25.01±0.12 Comparative Example 10 18.59±0.13 Comparative Example 11 28.69±0.22

[0092] Comparative Example 3, Comparative Example 8, Example 3, and Comparative Example 1 were compared. Comparative Example 3 added fennel essential oil to Comparative Example 1. The improvement in antibacterial effect brought about by the application of fennel essential oil was: against Helicobacter pylori, 17.12 mm (average inhibition zone of Comparative Example 3) - 12.65 mm (average inhibition zone of Comparative Example 1) = 4.47 mm. Comparative Example 8 added IgY to Comparative Example 1. The improvement in antibacterial effect brought about by the application of IgY was: against Helicobacter pylori, 23.13 mm (average inhibition zone of Comparative Example 8) - 12.65 mm (average inhibition zone of Comparative Example 1) = 10.48 mm. In Example 3, IgY and fennel essential oil were added to the results of Comparative Example 1. The improvement in antibacterial efficacy achieved by the addition of IgY and fennel essential oil was as follows: against Helicobacter pylori, 29.03 mm (average inhibition zone in Example 3) minus 12.65 mm (average inhibition zone in Comparative Example 1) equaled 16.38 mm, which was greater than the sum of the effects of either agent alone. This suggests a synergistic antibacterial effect between IgY and fennel essential oil.

[0093] Similarly, the above-mentioned synergistic effect was also observed when comparing Comparative Examples 2, 7, and Example 2 with Comparative Example 1, and when comparing Comparative Examples 9, 10, and Example 4 with Comparative Example 1. Therefore, in order to achieve a more ideal antibacterial effect, the addition amount of IgY and fennel essential oil is preferably 0.1-2 parts, more preferably 0.1-1 parts.

[0094] Experimental Example 3: Stability Test

[0095] Each experimental group of oral-detonating beads was sealed in boxes of 100 beads each and placed in a constant temperature and humidity chamber (40°C, 75% relative humidity) for 90 days. The appearance of the beads was observed and quality tested, and the breakage rate was calculated as: number of broken beads / 100 × 100%. Comparative Example 12 was also added: This comparative example was essentially the same as Example 2, except for a specific preparation process: in the preparation of the core liquid, the IgY-fennel essential oil coating solution was omitted, and the core liquid was obtained by directly mixing all materials in proportion. The remaining process was identical to Example 2. The experimental results are detailed in Table 6.

[0096] The stability of Examples 1 to 3 at room temperature and in the 90-day accelerated test is relatively good (the dosage of IgY and fennel essential oil is optimal at 0.01-1 parts). Although Example 4 has slight precipitation and discoloration, it is within the acceptable range and the breakage rate is controlled within 1%. As can be seen from Comparative Example 2, adding IgY alone without adding fennel essential oil will cause the oral popping beads to break, especially in the accelerated test, the breakage rate reaches 5%. In Comparative Examples 8 and 9, IgY is used alone without fennel essential oil, resulting in unsatisfactory stability of the popping beads and easy breakage. This shows that in order to avoid the instability of the dosage form caused by the addition of IgY and to improve the compatibility of IgY with the overall solution, it is necessary to use fennel essential oil to encapsulate IgY. As can be seen from Comparative Examples 4 and 5, if the IgY-fennel essential oil encapsulation liquid is not premixed at a 1:1 ratio, the appearance of the popping beads will be damaged. This shows that the ratio of IgY and fennel essential oil is very critical for maintaining the stability of the oral popping beads and the compatibility of the materials. At the same time, when the amount of IgY and fennel essential oil added is greater than 2% (Comparative Example 6), although the antibacterial effect is better, the appearance changes color and the breakage rate is as high as 20%. This shows that when IgY and fennel essential oil are added in a 1:1 ratio and the addition amount is 0.01-2% (especially 0.01-1%), the stability is better and the antibacterial effect is better. The premixing of oil-soluble fennel essential oil with water-soluble IgY can improve the stability of IgY in oral popping beads. The inventors analyzed that the reason is that this may be because the main chemical components of fennel essential oil, such as anisaldehyde, and IgY premix will form a complex in situ at the oil-water interface.

[0097] Comparative Example 11, using lemon essential oil instead of fennel essential oil, resulted in capsule damage and unsatisfactory appearance. This demonstrates the important role of fennel essential oil in stabilizing IgY encapsulation systems. Comparative Example 12, in which no IgY-fennel essential oil encapsulation solution was specifically prepared, failed to fully utilize the fennel essential oil's role in stabilizing IgY encapsulation systems, resulting in the resulting product being susceptible to damage.

[0098] Table 6: Stability test results

[0099]

[0100] Although this technical solution shows the specific preparation process of the popping beads, it needs to be further explained that the content of the popping beads is the core liquid, which can be packaged into the popping beads for easy use and transportation. The core liquid and the capsule skin have good compatibility. The popping beads are a specific application of the core liquid. Due to the formula of the core liquid, the overall stability of the popping beads is relatively ideal and not easy to break. The core liquid itself has the effect of antibacterial and refreshing breath. It can also be directly used as an oral antibacterial freshener product, and the core liquid is directly applied to the oral cavity (without using the popping beads as a carrier). This technical solution has fully demonstrated the beneficial effect of the core liquid on antibacterial, and because the core liquid contains ingredients such as menthol, it has the effect of removing breath. The core liquid can be used for direct gargling, which has the effect of antibacterial and refreshing breath, and the core liquid ingredients are safe and can be swallowed directly after gargling. Of course, the core liquid can also be prepared into products such as oral sprays according to conventional means of the existing technology to further exert the antibacterial and breath-freshening functions of the core liquid.

[0101] The above is only an embodiment of the present invention, and the common knowledge such as the specific structure and characteristics of the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several variations and improvements can be made without departing from the structure of the present invention, and these should also be regarded as the scope of protection of the present invention. These will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An IgY oral explosive bead with antibacterial effect, characterized by: The invention comprises a capsule skin; the capsule skin is wrapped with a core liquid; The raw materials of the core liquid include, by weight: 8-35 parts of natural menthol, 0.01-1 part of sucralose, 0.001-0.5 part of neotame, 1-20 parts of edible flavor, 0.01-1 part of IgY, and 0.01-1 part of fennel essential oil; The raw materials of the capsule shell include, by weight: 20-55 parts of gelatin, 0.01-1 parts of sucralose, 0.1-5 parts of erythritol, and 2-15 parts of glycerol; The raw materials of the core liquid and the capsule skin are dissolved in a solvent respectively; the solvent includes at least one of medium-chain triglycerides, walnut oil, coconut oil, and olive oil; the particle size of the IgY oral bursting beads is 4-10 mm; The core liquid is prepared by the following method: adding IgY while stirring fennel essential oil at a speed of 200 rpm-500 rpm in a temperature environment of 20-25°C, and continuing to stir at a speed of 700 rpm-1000 rpm for 5-10 min after the addition to form an IgY-fennel essential oil coating liquid; and mixing the IgY-fennel essential oil coating liquid with the remaining raw materials and solvent of the core liquid to obtain the core liquid.

2. The IgY oral explosive beads with antibacterial efficacy according to claim 1, characterized in that: The mass ratio of IgY and fennel essential oil was 1:

1.

3. Use of an oral care product comprising a composition having antibacterial efficacy in the preparation of oral popping beads, characterized in that: In parts by weight, the raw materials of the oral care product include 8-35 parts of natural menthol, 0.01-1 part of sucralose, 0.001-0.5 part of neotame, 1-20 parts of edible flavor, 0.01-1 part of IgY, and 0.01-1 part of fennel essential oil; The oral care product is prepared by the following method: adding IgY while stirring fennel essential oil at a speed of 200 rpm to 500 rpm in a temperature environment of 20-25° C., and continuing stirring at a speed of 700 rpm to 1000 rpm for 5-10 minutes after the addition to form an IgY-fennel essential oil coating liquid; and mixing the IgY-fennel essential oil coating liquid with the remaining materials of the oral care product to obtain the product; The oral explosive beads include a capsule, and the oral care product is wrapped in the capsule; The raw materials of the capsule shell include, by weight: 20-55 parts of gelatin, 0.01-1 parts of sucralose, 0.1-5 parts of erythritol, and 2-15 parts of glycerol; The raw materials of the oral care product and the capsule shell are respectively dissolved in a solvent; the solvent comprises at least one of medium-chain triglycerides, walnut oil, coconut oil, and olive oil.

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