Traditional Chinese medicine composition for radiation resistance and multi-system injury repair and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine composition for resisting radiation and repairing multi-system injury. The traditional Chinese medicine composition is prepared from fructus gardeniae, radix curcumae and flos lonicerae. The research proves that the traditional Chinese medicine composition of the fructus gardeniae, the radix curcumae and the lonicerae flos can repair multi-system injury after body radiation, effectively reduce the death rate caused by radiation, and well prolong the survival time of mice after radiation on the premise of ensuring the life quality. Specifically, the traditional Chinese medicine composition can enhance the immune system function, protect the bone marrow hematopoietic system and relieve renal function impairment. The invention provides a method for protecting healthy people from ionizing radiation injury by using the traditional Chinese medicine composition, and the traditional Chinese medicine composition can be used for resisting radiation, improving the lifetime, enhancing the immune system function, promoting bone marrow hematopoietic function recovery and protecting the kidney, and has important significance on improving the survival rate after radiation and reducing the death rate.

Description

Technical Field

[0001] The invention relates to a traditional Chinese medicine composition for repairing radiation damage, and specifically relates to an anti-radiation traditional Chinese medicine composition containing three ingredients: warm gardenia, warm curcuma and honeysuckle, and its application. Background Art

[0002] Radiation-induced multi-system damage to the body refers to the damage to two or more anatomical parts or organs caused by radiation factors at the same time or successively. After ionizing radiation acts on the body, it causes a series of physiological and even pathological changes, which can lead to multi-system damage to the body. Through complex physical, chemical and biological mechanisms, ionizing radiation first causes damage to macromolecules such as DNA, and then causes radiation damage to cells, tissues, organs and even systems and systemic functions and metabolism. The hematopoietic and immune systems are highly sensitive to radiation. After the body is irradiated with ionizing radiation, the blood system will soon have very obvious changes in morphology, function, quantity and characteristics, affecting the differentiation and growth of hematopoietic stem cells. The immune system will show a decrease in the number of immune cells, inhibition of antibody formation and inhibition of immune response, which will eventually lead to immune dysfunction and low immunity, reduce the body's ability to resist external invasion, and be easily infected with bacteria, viruses and other pathogens, resulting in a series of complications, which greatly affects the patient's quality of life and may accelerate the aging of the patient's body or even death. Kidneys are sensitive tissues in target organ damage after radiation effects. Radiation can cause pathological changes in kidney tissues, increase multiple oxidation indicators, and lead to the inability to excrete metabolites such as creatinine and uric acid in a timely manner, resulting in impaired renal function. Therefore, the repair of multi-system damage after radiation must be given high attention, such as the protection of the hematopoietic system, the reconstruction of immune function, and the recovery of renal function damage.

[0003] With the widespread application of atomic energy technology, more and more workers are facing health risks due to accidental exposure to ionizing radiation. Therefore, improving the ability of relevant personnel to resist ionizing radiation has become an urgent research goal. At present, radiation protection mainly relies on external protection tools and anti-radiation drugs. However, protective clothing and other equipment cannot achieve intrinsic protection. Although existing synthetic anti-radiation agents, such as thiol compounds, nitroxide free radicals and dibenzimidazoles, focus on scavenging free radicals, inhibiting cell metabolism and repairing damaged cells, most of them are highly toxic to healthy cells, easily induce other lesions, and have unsatisfactory efficacy. In addition, these compounds are usually expensive and require large doses. Therefore, it is of great significance to develop new protective agents that are efficient, low-toxic, inexpensive and have multi-target effects. Traditional Chinese medicine has the characteristics of being derived from natural plants, rich in resources, low in toxicity and side effects, and suitable for long-term application. Some single Chinese medicines can significantly protect hematopoietic cells and hematopoietic tissues, while others can promote cell repair and functional recovery. Compared with single ingredients, Chinese herbal compound prescriptions can show advantages such as synergistic enhancement, multi-target regulation, and toxicity reduction and efficacy enhancement through the rational combination of multiple Chinese medicinal materials. The theoretical basis of Chinese herbal compound prescriptions comes from the holistic concept of traditional Chinese medicine, combined with modern pharmacological research, and its characteristics are to reduce the toxic side effects that may be caused by single herbs and enhance the efficacy.

[0004] In traditional Chinese medicine, Wen Zhizi is called "Gardenia" or "Shan Zhi". It tastes bitter and is cold in nature. It enters the heart, lung and triple burner meridians. The lung runs through the heart meridian to circulate qi and blood. If the heart qi is insufficient, blood circulation will be blocked. The upper heart, the middle spleen and stomach, and the lower liver and kidneys. The triple burner is the "general manager of the five internal organs and six bowels" and can communicate the airway, waterway and bloodway. The main components of Wen Zhizi include iridoid glycosides, flavonoids, phenylethanol glycosides, triterpenoids, organic acids, essential oils, etc., which have multiple effects such as anti-inflammatory, antioxidant, antiviral, anti-tumor and liver protection. The dried and mature fruits of Gardenia jasminoides are used as medicine, which has the effects of protecting the liver and promoting bile secretion, purging fire and relieving restlessness, cooling blood and detoxifying, clearing away heat and dampness, and is used for heat pathogenic heat invading the heart, chest distress, restlessness and insomnia, bladder heat due to stagnation of heat and dampness, jaundice, fever, short and red urine caused by stagnation of heat and dampness in the liver and gallbladder, and blood-stained stranguria, dribbling and painful urination, vomiting and bleeding due to blood heat, and red and swollen eyes caused by stagnation of heat in the bladder. Mashing raw Gardenia jasminoides for external application has the effect of clearing heat, reducing swelling and relieving pain, and can be used for sprains and contusions.

[0005] Curcuma wenyujin is called "Yujin" in traditional Chinese medicine. It tastes spicy and bitter, is cold in nature, and enters the liver, heart, and lung meridians. It is mainly used to promote qi and relieve depression, promote blood circulation and relieve pain, and promote bile and reduce jaundice. It is used to treat chest, abdomen, rib pain, insanity, fever, coma, hematemesis, epistaxis, hematuria, hematuria, retrograde menstruation in women, and jaundice. The main components of Curcuma wenyujin include Curdione, Curmol, Curzerene, Curcumin, etc. These components have anti-inflammatory, anti-tumor, antioxidant and immunomodulatory effects. It has been reported that Curcuma wenyujin polysaccharide has the effects of enhancing immunity and anti-tumor.

[0006] Honeysuckle is the dried buds or newly opened flowers of the honeysuckle plant of the Caprifoliaceae family, and is a type of Chinese medicinal material "honeysuckle". Honeysuckle is sweet in taste, cold in nature, and enters the lung, heart, and stomach meridians. It has the functions of clearing away heat and detoxifying, and evacuating wind and heat. It is used to treat pain and swelling, sores, throat paralysis, erysipelas, heat-toxic bloody diarrhea, wind-heat colds, and febrile diseases. The main components of honeysuckle are flavonoids, organic acids, volatile oils, saponins, polysaccharides and other ingredients, which are mainly related to anti-inflammatory, antibacterial and antioxidant effects and have immunomodulatory, anti-inflammatory and anti-tumor effects.

[0007] Ionizing radiation belongs to the category of "fire, heat and toxic evil" in traditional Chinese medicine. Fire is a yang evil, which boils body fluids, and forms pathological products such as blood stasis and blood reduction over time. Fire is easy to consume qi and damage body fluids. Blood is formed by the transformation of water, grain and body fluids by the spleen and the redness of heart yang. The reduction of body fluids leads to insufficient blood production, which in turn leads to a decrease in peripheral blood cells. Fire is easy to enter the camp and move blood, forcing blood to flow indiscriminately, leading to various bleeding. In short, ionizing radiation can cause the body's qi, blood and body fluids to be deficient, and the balance of yin and yang is unbalanced. The application of Chinese medicine compositions in radiation protection mainly takes "strengthening the body and eliminating evil" as the treatment principle, which is used to replenish qi and blood, replenish essence and fill the marrow, improve the body's immune ability, and improve hematopoietic microcirculation. The polysaccharides, flavonoids and other components in warm gardenia, warm turmeric and honeysuckle can improve the body's hematopoietic ability, promote the release of immune cells in the bone marrow periphery, enhance the activity of macrophages, inhibit the production and release of inflammatory mediators, and enhance the function of the immune system, thereby having a certain repair effect on the body's damage after radiation. In addition to the effects of clearing away heat and detoxicating, purging fire and improving eyesight, the two of Warm Gardenia and Warm Curcuma can also strengthen the effects of antibacterial and anti-inflammatory, improving blood circulation, etc. in cooperation with each other. Warm Curcuma and Flos Honeysuckle have the effects of clearing away heat and detoxicating, cooling blood and dispersing blood stasis, and promoting qi and relieving pain. The two are compatible, and the heat-clearing and detoxicating effect of Flos Honeysuckle can assist Warm Curcuma in promoting qi and activating blood circulation, while the warm nature of Warm Curcuma can neutralize the cold nature of Flos Honeysuckle, so that it is not too cold. This compatibility has a significant effect in treating heat-toxic blood stasis, qi stagnation and pain, etc., and is suitable for symptoms such as pain and lumps caused by heat-toxic diarrhea, heat-toxic sores, and qi stagnation and blood stasis. In addition, Warm Gardenia is also often compatible with Flos Honeysuckle in terms of clearing heat and purging fire, cooling blood and detoxifying. These three herbs each have unique meridians and functions in traditional Chinese medicine theory. Through scientific compatibility, they not only enhance the repair effect of multiple system damage after radiation, but also reduce the adverse reactions that may be caused by the use of a single drug, providing theoretical support and practical basis for the development of highly effective and low-toxic natural anti-radiation Chinese medicine compositions.

[0008] With the advancement of the modernization of traditional Chinese medicine, Chinese medicinal materials with many active ingredients, significant anti-radiation effects, no toxic reactions or mild toxic reactions have broad prospects for research and development as anti-radiation drugs. Based on the scientific compatibility of traditional Chinese medicine theory, the Chinese medicine composition has more comprehensive performance in the protection of the hematopoietic system, immune regulation and kidney function recovery after radiation. Summary of the invention

[0009] The purpose of the present invention is to provide an anti-radiation Chinese medicine composition and a preparation method thereof, wherein the anti-radiation Chinese medicine composition can repair multi-system damage of the body caused by radiation, thereby achieving an effective anti-radiation effect.

[0010] In a first aspect, the present invention provides an anti-radiation Chinese medicine composition, the Chinese medicine composition comprises Gardenia jasminoides, Curcuma zedoariae and Honeysuckle, and the weight proportions of each raw material are as follows:

[0011] Warm Gardenia: 10 parts by weight;

[0012] Curcuma wenjin: 55 weight method;

[0013] Honeysuckle: 17 parts by weight.

[0014] In the second aspect, the present invention also discloses a method for preparing the above-mentioned radiation-resistant Chinese medicine composition, which should also fall within the protection scope of the present invention. The preparation method specifically comprises the following steps:

[0015] According to the prescription, 4.0 grams of Gardenia jasminoides, 21.88 grams of Curcuma zedoariae and 6.7 grams of Honeysuckle were taken, chopped and soaked in 200 ml of ultrapure water for 30 minutes, decocted for the first time with high heat for 30 minutes, and the upper layer of liquid was poured out, i.e., the first decoction; 150 ml of water was added to continue decocting for 30 minutes, the upper layer of liquid was poured out, the residue was wrapped with gauze to filter the residue, and the second decoction was obtained. The first decoction and the second decoction were mixed, the liquid was cooled and centrifuged at 4500 rpm for 15 minutes, filtered with three layers of gauze to obtain the final decoction, and then stored in a refrigerator at 4°C to obtain the radiation-resistant Chinese medicine composition of the present invention.

[0016] The application of the above-mentioned anti-radiation Chinese medicine composition in medicines and health care products should also fall within the protection scope of the present invention.

[0017] The present invention also provides an anti-radiation Chinese medicine preparation, which specifically comprises the above-mentioned anti-radiation Chinese medicine composition, prepared into a preparation according to a conventional method.

[0018] The advantages of the radiation-resistant Chinese medicine composition preparation provided by the present invention are as follows:

[0019] 1. The anti-radiation Chinese medicine composition provided by the present invention has a repairing effect on multi-system damage after radiation of the body, intervenes in radiation-induced damage to the immune system, bone marrow hematopoietic system and kidney organs, improves the quality of life, and achieves an effective anti-radiation effect.

[0020] 2. The anti-radiation Chinese medicine composition provided by the present invention can significantly improve the decrease in peripheral blood leukocyte and platelet counts caused by radiation damage, reduce the degree of immune cell damage, and enhance the function of the immune system.

[0021] 3. The radiation-resistant Chinese medicine composition provided by the present invention can effectively reduce the formation rate of bone marrow micronuclei after radiation and protect the hematopoietic function of the bone marrow.

[0022] 4. The anti-radiation Chinese medicine composition provided by the present invention can regulate the body's creatinine level after radiation, reduce renal function damage after radiation, and play a certain protective role on the kidneys.

[0023] 5. The radiation-resistant Chinese medicine composition of the present invention has a precise radiation-resistant effect, is simple to prepare, and is easy to take, and has a good prospect in preventing and treating radiation damage. DETAILED DESCRIPTION

[0024] The following are specific embodiments of the present invention, which further describe the technical solution of the present invention, but the protection scope of the present invention is not limited to these embodiments. Any changes or equivalent substitutions that do not deviate from the concept of the present invention are included in the protection scope of the present invention.

[0025] The experimental methods used in the following examples are conventional methods unless otherwise specified. All the parts involved are by weight unless otherwise specified. The preparation prepared from the radiation-resistant Chinese medicine composition of the present invention is a liquid obtained from pharmaceutically acceptable excipients, which is a decoction after the raw materials are processed, decocted and filtered.

[0026] Example 1

[0027] The present invention provides an anti-radiation Chinese medicine composition, wherein the weight proportions of the raw materials are as follows:

[0028]

[0029] The preparation method of the radiation-resistant Chinese medicine composition is as follows:

[0030] According to the prescription, 4.0 grams of Gardenia jasminoides, 21.88 grams of Curcuma zedoariae and 6.7 grams of Honeysuckle were taken, chopped and soaked in 200 ml of ultrapure water for 30 minutes, decocted for the first time with high heat for 30 minutes, and the upper layer of liquid was poured out, i.e., the first decoction; 150 ml of water was added to continue decocting for 30 minutes, the upper layer of liquid was poured out, the residue was wrapped with gauze to filter the residue, and the second decoction was obtained. The first decoction and the second decoction were mixed, the liquid was cooled and centrifuged at 4500 rpm for 15 minutes, filtered with three layers of gauze to obtain the final decoction, and then stored in a refrigerator at 4°C to obtain the radiation-resistant Chinese medicine composition of the present invention.

[0031] Example 2 Effect of the Chinese medicine composition of the present invention on the mortality rate and body weight change of mice

[0032] In order to study the anti-radiation effect of the anti-radiation Chinese medicine composition of the present invention on survival conditions, a study was conducted.

[0033] 1. Experimental Methods

[0034] 1.1 Animal husbandry and grouping

[0035] 1.1.1 Experimental conditions

[0036] Seventy 6-8 week old SPF grade ICR male mice weighing 30±2 g each were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. and were raised under standard experimental conditions of free access to food and water at a temperature of 25±2°C, a humidity of 80±5% and a pressure of 90.0 kpa. The mice were randomly divided into 7 groups, with 10 mice in each group.

[0037] 1.1.2 Group Processing

[0038] According to the principles of Experimental Pharmacology Methodology, the dosing levels for the experimental group and the positive control group were calculated according to the clinical dosage for adults, and the radiation protection drugs for the following treatments were prepared.

[0039] After one week of adaptation, mice were gavaged once a day for one week before the irradiation experiment, and whole-body irradiation was performed 30 minutes after the last administration. During irradiation, mice were fixed in resin cages with air holes (or fixed in special plexiglass boxes), and animals were irradiated with 7.0Gy of Se137-γ rays using HXFS-IA blood irradiator at the Southern Zhejiang Institute of Radiation Medicine and Nuclear Technology. Mice in the blank control group were operated in the same way but not irradiated.

[0040] Treatment 1: Take 4.0 grams of Gardenia jasminoides, 21.88 grams of Curcuma zedoariae and 6.7 grams of Honeysuckle Flos according to the prescription, chop them and soak them in 200 ml of ultrapure water for 30 minutes, decoct them for the first time with high heat for 30 minutes, pour out the upper layer of liquid, i.e., the first decoction; add 150 ml of water and continue to decoct for 30 minutes, pour out the upper layer of liquid, wrap the residue with gauze to filter the residue, and combine to obtain the second decoction. The first decoction and the second decoction are mixed, the liquid is cooled and centrifuged at 4500 rpm for 15 minutes, filtered with three layers of gauze to obtain the final decoction, and then stored in a refrigerator at 4°C to obtain the radiation-resistant Chinese medicine composition of the present invention.

[0041] Treatment 2: Take 4.0 g of Wenzhizi medicinal material according to the prescription, chop it and soak it in 200 ml of ultrapure water for 30 minutes, decoct it for the first time with high heat for 30 minutes, pour out the upper layer of liquid, that is, the first decoction; add 150 ml of water and continue to decoct for 30 minutes, pour out the upper layer of liquid, wrap the residue with gauze to filter the residue, and combine to obtain the second decoction. The first decoction and the second decoction are mixed, and the liquid is cooled and centrifuged at 4500 rpm for 15 minutes, filtered with three layers of gauze to obtain the final decoction, and then stored in a refrigerator at 4°C. When used, add 0.02 g / 100 ml of vitamin C solution of corresponding weight parts and mix well. The obtained Chinese medicine composition is recorded as Wenzhizi + Vitamin C group.

[0042] Treatment 3: Take 21.875 grams of Wenyujin medicinal materials according to the prescription, chop them and soak them in 200 ml of ultrapure water for 30 minutes, decoct them for the first time with high heat for 15 minutes, pour out the upper layer of liquid, that is, the first decoction; add 150 ml of water and continue to decoct for 15 minutes, pour out the upper layer of liquid, wrap the residue with gauze to filter the residue, and combine to obtain the second decoction. The first decoction and the second decoction are mixed, and the liquid is cooled and centrifuged at 4500 rpm for 15 minutes, filtered with three layers of gauze to obtain the final decoction, and then stored in a refrigerator at 4°C. When used, add 0.02 g / 100 ml of vitamin C solution of corresponding weight parts and mix well. The obtained Chinese medicine composition is recorded as Wenyujin + vitamin C group.

[0043] Treatment 4: Take 6.7 grams of honeysuckle according to the prescription, chop it and soak it in 200 ml of ultrapure water for 30 minutes, decoct it for the first time with high heat for 15 minutes, pour out the upper layer of liquid, that is, the first decoction; add 150 ml of water and continue to decoct for 15 minutes, pour out the upper layer of liquid, wrap the residue with gauze to filter the residue, and combine to obtain the second decoction. The first decoction and the second decoction are mixed, and the liquid is cooled and centrifuged at 4500 rpm for 15 minutes, filtered with three layers of gauze to obtain the final decoction, and then stored in a refrigerator at 4°C. When used, add the corresponding weight portion of 0.02 g / 100 ml vitamin C solution and mix well. The obtained Chinese medicine composition is recorded as honeysuckle + vitamin C group.

[0044] Treatment 5: 0.02 g / 100 ml vitamin C solution was used as the radiation protection drug, recorded as the vitamin C group.

[0045] Treatment 6: an equal amount of distilled water, recorded as the radiation control group.

[0046] Treatment 7: equal amount of distilled water, no irradiation, other treatments were the same as treatments 1-5, recorded as blank control group.

[0047] 2. Experimental results

[0048] After 15 days of treatment with the Chinese medicine composition of the present invention, the survival rate of mice and the changes in body weight with prolonged irradiation time are shown in Table 1-2 below.

[0049] Table 1 15-day survival rate of mice

[0050]

[0051] Table 2 Changes in mouse body weight with prolonged irradiation time

[0052]

[0053] As shown in Table 1, the anti-radiation Chinese medicine composition of the present invention can effectively reduce the mortality of acutely irradiated mice, better prolong the survival time of mice after radiation, and improve the survival rate of mice after radiation. Combined with the weight changes in Table 2, it can be seen that the anti-radiation Chinese medicine composition of the present invention is more effective in alleviating the weight loss of mice after irradiation, and can improve the quality of life of mice to a certain extent.

[0054] Example 3 Effect of the Chinese medicine composition of the present invention on the bone marrow hematopoietic function of mice

[0055] In order to study the protective effect of the radiation-resistant Chinese medicinal composition of the present invention on bone marrow hematopoietic function, a study was conducted.

[0056] 1. Experimental Methods

[0057] The feeding and grouping of experimental animals were the same as in Example 2.

[0058] 1.1 Blood and bone marrow collection

[0059] 15 days after the experimental animals were irradiated, their eyeballs were removed and blood was collected using blood collection tubes and then processed and stored as soon as possible according to the standard blood sample processing procedures.

[0060] Kill the mice by the spinal cord injury method, then cut the skin and muscles on the thigh with scissors, remove the thigh bone, and use a small piece of gauze to rub the muscle debris attached to the bone. Cut off the enlarged joint heads at both ends of the femur, then use a 2 ml syringe to absorb 2 ml of Hanks solution, insert it into one end of the femur, and flush the bone marrow cells into a 2 ml centrifuge tube. Repeat the flushing several times until the bone marrow cavity turns white.

[0061] 1.2 Centrifugation

[0062] The obtained cell suspension was centrifuged at 1000 rpm for 10 minutes, the supernatant was removed by aspiration, and 2 drops of inactivated calf serum were added to the precipitate to prepare a cell suspension.

[0063] 1.3 Smear

[0064] Place a drop of the suspension on one end of the slide, smear it in the usual way, and let it dry in the air.

[0065] 1.4 Fixed

[0066] Place the dried slides in methanol fixative for 10 minutes and take them out to dry.

[0067] 1.5 Dyeing

[0068] Place the slide flat on a glass plate, stain with Giemsa for 15 minutes, rinse with running water, dry and then examine under a microscope.

[0069] 1.6 Observation

[0070] First observe under a low power microscope, select the area with even distribution and good staining, and then observe and count under an oil microscope. Polychromatic erythrocytes (PCE) are gray-blue, and orthochromatic erythrocytes (NCE) are orange-yellow. Most of the micronuclei contained in the cells are round, with smooth and neat edges, and the color is consistent with the nucleoplasm, which is purple-red or blue-purple.

[0071] 1.7 Count

[0072] One or more micronuclei may appear in a cell. Count the number of PCEs containing micronuclei among 2000 PCEs, calculate the micronucleus cell rate, and observe and record the results.

[0073] 2. Experimental results

[0074] After 15 days of treatment with the Chinese medicine composition of the present invention, the changes in the micronucleus formation rate in mice are shown in Table 3 below.

[0075] Table 3 Statistics of micronucleus formation rate

[0076]

[0077] Radiation can lead to a decrease in hematopoietic cells in bone marrow cells. The results of the micronucleus test show (Table 3) that the radiation-resistant Chinese medicine composition of the present invention can effectively reduce the micronucleus formation rate in bone marrow cells and has a good protective effect on radiation-induced bone marrow damage and hematopoietic function.

[0078] Example 4 Effect of the Chinese medicine composition of the present invention on peripheral blood count of mice

[0079] In order to study the protective effect of the anti-radiation Chinese medicinal composition of the present invention on the immune system and blood system functions, the peripheral blood picture thereof was studied.

[0080] 1. Experimental Methods

[0081] The feeding and grouping of experimental animals were the same as in Example 2, and the blood sample collection and processing were the same as in Example 3.

[0082] 2. Experimental results

[0083] After 15 days of treatment with the Chinese medicine composition of the present invention, the changes in the blood routine of mice are shown in the following Table 4-5.

[0084] Table 4 Statistics of blood routine indicators

[0085]

[0086] Table 5 Comparison of blood routine indicators

[0087]

[0088] The change of whole blood cells can reflect the degree of radiation damage suffered by the body, which is specifically manifested as the inhibition of the generation of white blood cells, red blood cells and platelets. As shown in Tables 4-5, the white blood cell, red blood cell and platelet counts of each group of mice after irradiation were significantly reduced compared with the blank control group, and the difference was statistically significant. Compared with the radiation control group, the white blood cell, red blood cell and platelet counts of the anti-radiation Chinese medicine composition of the present invention were all increased, and the difference between the white blood cell count and the platelet count was statistically significant. It is shown that the anti-radiation Chinese medicine composition of the present invention can increase the number of peripheral blood cells, reduce the damage caused by radiation to the peripheral blood of mice, and have a protective effect on the blood system of mice after irradiation. On the other hand, when the number of white blood cells belonging to immune cells is significantly reduced, it indicates that the body's immune function has been disordered. The anti-radiation Chinese medicine composition of the present invention can significantly improve the situation of leukopenia, reduce the degree of damage to immune cells, and enhance its immune system function. At the same time, the anti-radiation Chinese medicine composition of the present invention can significantly improve the reduction of platelet count, so that enough platelets participate in immune regulation, so that the peripheral blood leukocytes of mice are further repaired and increased.

[0089] Example 5 Effects of the Chinese medicine composition of the present invention on the kidney

[0090] In order to study the protective effect of the anti-radiation Chinese medicinal composition of the present invention on kidney, the creatinine index was studied.

[0091] 1. Experimental Methods

[0092] The feeding and grouping of experimental animals were the same as in Example 2, and the blood sample collection and processing were the same as in Example 3.

[0093] 2. Experimental results

[0094] After 15 days of treatment with the Chinese medicine of the present invention, the changes in the creatinine index of mice are shown in Table 6-7 below.

[0095] Table 6 Creatinine index of mice

[0096]

[0097] Table 7 Comparison of creatinine indicators

[0098]

[0099] Creatinine is a product of muscle metabolism, which is mainly filtered by the kidneys and excreted from the body in the form of urine. Therefore, the level of creatinine can reflect the functional state of the kidneys. As shown in Table 7, the creatinine level of mice after radiation was significantly higher than that of the blank control group, and the difference was statistically significant. Compared with the radiation control group, the creatinine level of the anti-radiation Chinese medicine of the present invention was significantly increased, but it was still higher than the creatinine level of the blank control group, and the difference was statistically significant. It shows that the anti-radiation Chinese medicine of the present invention can reduce the creatinine level of mice to a certain extent and reduce the damage of radiation to the kidneys of mice.

[0100] In summary, the radiation protection Chinese medicine composition of the present invention has a significant effect in saving the survival rate of acute radiation damage, and the Chinese medicine composition of the present invention has a repairing effect on multi-system damage after radiation, can significantly reduce the degree of damage to immune cells to enhance the function of the immune system, can significantly reduce the rate of bone marrow micronucleus formation to protect the bone marrow hematopoietic system, and can regulate creatinine levels to play a certain protective role on the kidneys.

[0101] The above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit the technical solutions. Any technical solutions within the scope of the present invention or any improvements and modifications that do not deviate from the principle of the present invention shall fall within the protection scope of the present invention.

Claims

1. A Chinese medicine composition for radiation resistance and multi-system damage repair, characterized in that: The traditional Chinese medicine composition comprises warm gardenia, warm turmeric and honeysuckle.

2. The Chinese medicine composition according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 10 parts by weight of warm gardenia medicinal material, 55 parts by weight of warm turmeric medicinal material and 17 parts by weight of honeysuckle medicinal material.

3. The Chinese medicine composition according to claim 1, characterized in that The Chinese medicine composition has a repairing effect on multi-system damage after radiation, which is mainly reflected in the immune system, bone marrow hematopoietic system and kidney organs, thereby improving the quality of life and achieving an effective anti-radiation effect.

4. The effect of the Chinese medicine composition according to claim 3 on the immune system, characterized in that: The Chinese medicine composition can significantly improve the decrease in peripheral blood leukocyte and platelet counts caused by radiation damage, reduce the degree of damage to immune cells, and enhance the function of the immune system.

5. The effect of the Chinese medicine composition according to claim 3 on the bone marrow hematopoietic system, characterized in that: The traditional Chinese medicine composition can effectively reduce the formation rate of bone marrow micronuclei and protect the hematopoietic function of the bone marrow.

6. The effect of the Chinese medicine composition according to claim 3 on the kidney organ, characterized in that: The Chinese medicine composition can regulate creatinine levels and play a certain protective role on the kidneys.

7. A method for preparing a radiation-resistant Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: taking 4.0 g of Gardenia jasminoides, 21.88 g of Gardenia jasminoides and 6.7 g of Lonicera japonica, chopping them and soaking them in 200 ml of ultrapure water for 30 minutes, decocting them for the first time with high heat for 30 minutes, pouring out the upper layer of liquid, i.e., the first decoction; adding 150 ml of water and continuing to decoct for 30 minutes, pouring out the upper layer of liquid, wrapping the medicinal residue with gauze to filter the residue, and combining them to obtain the second decoction. The first decoction and the second decoction are mixed, the medicinal liquid is cooled and centrifuged at 4500 rpm for 15 minutes, filtered with three layers of gauze to obtain the final decoction, and then stored in a refrigerator at 4°C to obtain the radiation-resistant Chinese medicine composition of the present invention.

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