A high temperature bio-enzyme plugging remover and its application

Abstract

A high-temperature biological enzyme plugging remover and its application belong to the technical field of biological oil production. The high-temperature biological enzyme plugging remover is composed of the following components by weight: 1-20 parts of biological enzyme preparation, 1-40 parts of biological active agent, 1-40 parts of biological organic acid, and 0-97 parts of water. The application of the high-temperature biological enzyme plugging remover is suitable for unblocking water injection wells and oil production wells on land and at sea. The plugging remover does not undergo chemical reaction during application, can be recycled and reused, and has extremely low operating costs; it is green and environmentally friendly, has good safety and stability, does not harm operators, and does not pollute the environment; it does not corrode oil and gas field pipelines and pipe columns; the working fluid has good compatibility with formation fluids and will not cause secondary pollution to the reservoir; it has a wider range of applications and is suitable for water injection wells and oil production wells in onshore and offshore oil fields; the biological enzyme protective agent realizes the temperature resistance and salt resistance of the biological enzyme, effectively solving the problems of reduced enzyme activity; it has lower cost, lower use concentration, wider adaptability, higher economic benefits, and is more conducive to promotion and application.

Description

Technical Field

[0001] The invention belongs to the technical field of biological oil recovery, and in particular relates to a high-temperature biological enzyme plugging remover and application thereof. Background Art

[0002] During oil well fracturing, drilling and completion, and long-term production, organic macromolecules will be deposited in the area near the wellbore, causing reservoir blockage, reduced permeability, and seriously affecting oil well production.

[0003] Initially, bio-enzyme plugging removal technology was applied in oil and gas field production to solve the problem of polymer debonding. In the past, production-increasing measures often used chemical plugging removal technology to remove blockages such as high-molecular polymers, clays, and oil scales, which had the problem of causing potential secondary damage to the reservoir. Therefore, researchers at home and abroad proposed the use of highly specific and environmentally friendly bio-enzyme plugging removal technology as a new idea for environmentally friendly plugging removal technology.

[0004] Combined with the current application status of bio-enzyme plugging removal technology, the technology mainly has the following problems: (1) Bio-enzyme products have poor salt and high temperature resistance. This is because the essence of enzymes is protein, which has the characteristics of protein. Once the external environment reaches its denaturation conditions, the functional groups of the enzymes will lose their activity. The temperature resistance of enzyme products currently used on site is generally lower than 70 ℃, and they are more sensitive to mineralization. Most enzyme products have salt precipitation when the mineralization is above 5000 mg / L, resulting in reduced enzyme activity or even inactivation; (2) The cost of enzyme products is relatively high.

[0005] However, with the continuous improvement of national environmental protection requirements, bio-enzyme plugging removal technology has been widely recognized as an effective and environmentally friendly measure to increase production and injection. Bio-enzyme plugging removal technology will be a major focus of the future series of production and injection technology. For this reason, it is very necessary to provide a salt-resistant and temperature-resistant bio-enzyme plugging remover. Summary of the invention

[0006] The present invention aims to overcome the shortcomings of the prior art and provides a high-temperature bio-enzyme plugging remover and its application, which is low-cost, green and environmentally friendly, salt-resistant and temperature-resistant, and can greatly improve the temperature and salt resistance compared with existing products or technologies. The cost is much lower than the cost required for technical approaches such as genetic modification, and is more conducive to mine application.

[0007] The high-temperature bio-enzyme plugging remover of the present invention is composed of the following components in parts by weight: 1 to 20 parts of bio-enzyme preparation, 1 to 40 parts of bio-active agent, 1 to 40 parts of bio-organic acid, and 0 to 97 parts of water; The biological enzyme preparation is composed of the following components in parts by weight: 1-20 parts of biological complex enzyme, 1-20 parts of biological enzyme protective agent and 60-98 parts of water.

[0008] As a further improvement of the present invention, the biological complex enzyme is a mixture of two or more of protein complex enzyme, cellulase, hemicellulase, xanthan gum lyase, β-mannanase and lipase in any proportion.

[0009] As a further improvement of the present invention, the biological enzyme protectant is a combination of a biological enzyme antioxidant, a biological enzyme emulsifier, and a biological enzyme stabilizer, with the ratio being 2:1:1.

[0010] As a further improvement of the present invention, the bio-enzyme antioxidant is a mixture of one or more of vitamin C, vitamin E, trehalose, gelatin polysaccharide and polyethylene glycol in any proportion.

[0011] As a further improvement of the present invention, the bio-enzyme emulsifier is composed of one or more of sucrose ester, fatty acid glyceride, hexadecyltrimethylammonium chloride, stearic acid triethanolamine salt, and benzalkonium bromide mixed in any proportion.

[0012] As a further improvement of the present invention, the biological enzyme stabilizer is composed of one or more of sodium acetate, sodium citrate, sodium nitrate, calcium sulfate and potassium phosphate mixed in any proportion.

[0013] As a further improvement of the present invention, the bioactive agent is a mixture of one or two of lipopeptide surfactant and phenyl octadecylaminopropyl dimethylaminoacetate in any proportion.

[0014] As a further improvement of the present invention, the biological organic acid is a mixture of one or more of lactic acid, formic acid, acetic acid, propionic acid, oxalic acid and benzoic acid in any proportion.

[0015] The application of the high-temperature bio-enzyme plugging remover of the present invention has high salt tolerance up to 200000 mg / L and is suitable for oil reservoir temperature range of 20-100°C.

[0016] The application of the high-temperature bio-enzyme plugging remover of the present invention is suitable for unblocking water injection wells and oil production wells on land and offshore.

[0017] The following materials used in the present invention are described below: Biocomplex enzyme: degrades organic scale in the formation through the action of enzymes; Bio-enzyme antioxidant: stabilizes the molecular structure of the enzyme, allowing it to maintain activity in harsh environments such as high temperature and oxidation; Bio-enzyme emulsifier: can reduce the tension on the oil-water interface, making the environment of the enzyme more stable, thereby extending its service life and life; Bio-enzyme stabilizer: by changing the local environment of the enzyme, making its structure more stable, thereby improving the enzyme's temperature resistance and salt resistance; the above-mentioned bio-enzyme antioxidant, bio-enzyme emulsifier and bio-enzyme stabilizer are combined to protect the bio-composite enzyme, making it salt-resistant and temperature-resistant and maintaining its activity; Bioactive agents: reduce the interfacial tension between crude oil and rock, strip crude oil and other deposited organic matter from the rock surface, improve recovery, increase formation permeability, and gather and flow out at the same time; Biological organic acid: dissolves and destroys inorganic scale in the formation.

[0018] The high-temperature bio-enzyme plugging remover of the present invention has the following advantages compared with the prior art system: 1. No chemical reaction occurs during the application process, it is a biochemical reaction. When the reaction is over, the chemical properties and quantity of the biological enzyme remain unchanged and the molecular structure is not destroyed, so the enzyme can be recycled and reused, and the operating cost is extremely low; 2. Green and environmentally friendly, safe and stable, no harm to operators and no pollution to the environment; 3. No corrosion to oil and gas field pipelines and pipe columns; 4. The working fluid has good compatibility with the formation fluid and will not cause secondary pollution to the reservoir; 5. It has a wider application range, with a high salt tolerance of up to 200,000 mg / L. It is also suitable for reservoir temperatures ranging from 20 to 100°C, and is suitable for water injection wells and oil production wells in onshore and offshore oil fields; 6. Use biological enzyme protective agents to achieve temperature and salt resistance of biological enzymes, effectively solving problems such as reduced enzyme activity; 7. Lower cost, lower usage concentration, wider adaptability, higher economic benefits, and more conducive to promotion and application. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical scheme and advantages of the invention more clearly understood, the present invention is further described in detail below in conjunction with embodiments. The illustrative embodiments of the present invention and their description are only used to explain the present invention and are not intended to limit the present invention.

[0020] Example 1

[0021] A high-temperature bio-enzyme plugging remover of the present invention is composed of the following components in parts by mass: 10 parts of biological enzyme preparation, 40 parts of biological active agent (lipopeptide surfactant), 10 parts of biological organic acid (acetic acid), 40 parts of water; The bio-enzyme preparation is made of 10 parts of bio-complex enzyme, 10 parts of bio-enzyme protective agent and 80 parts of water; The biological complex enzyme is prepared by mixing protein complex enzyme, xanthan gum lyase, β-mannanase and lipase in a ratio of 1:1:1:1; The biological enzyme protective agent is made of a biological enzyme antioxidant (vitamin E and trehalose in a ratio of 1:1), a biological enzyme emulsifier (fatty acid glyceride and stearic acid triethanolamine salt in a ratio of 1:1), and a biological enzyme stabilizer (sodium acetate and potassium phosphate in a ratio of 1:1), and the mixing ratio is 2:1:1; The preparation method is as follows: water, biological enzyme protective agent, biological complex enzyme, bioactive agent, and biological organic acid are added to the reactor in order according to mass proportion, the temperature is 25-40°C, the stirring speed is 50-80rpm, and the stirring time is 20-30min until completely dissolved to prepare a high-temperature biological enzyme plugging remover.

[0022] The onshore Daqing Oilfield oil well unblocking, the reservoir temperature is 45 ° C, the mineralization is about 15000 mg / L, the high temperature biological enzyme unblocking agent prepared in Example 1 is used to unblock and increase production, the high temperature biological enzyme unblocking agent is diluted with water to a mass concentration of 1% aqueous solution, and a pump truck is quickly injected into 150-500m 3 , replace with clean water and shut down the well for 3 to 7 days. After the well is opened, the average daily liquid production increases by more than 1 times, and the daily oil production increases by more than 1 times. The effective period is more than 180 days. The average cumulative oil production increase of a single well is more than 300t, and the highest cumulative oil production increase of a single well during the effective period is 495t.

[0023] Example 2

[0024] A high-temperature bio-enzyme plugging remover of the present invention is composed of the following components in parts by mass: 20 parts of biological enzyme preparation, 35 parts of biological active agent (lipopeptide surfactant and phenyl octadecylaminopropyl dimethylaminoacetate in a ratio of 1:1), 5 parts of biological organic acid (acetic acid, formic acid, lactic acid in a ratio of 1:1:1), 40 parts of water; The bio-enzyme preparation is made of 15 parts of bio-complex enzyme, 15 parts of bio-enzyme protective agent and 70 parts of water; The biological complex enzyme is prepared by mixing protein complex enzyme, xanthan gum lyase, β-mannanase, lipase and cellulase in a ratio of 1:2:2:1:1; The biological enzyme protective agent is a combination of a biological enzyme antioxidant (vitamin E and gelatin polysaccharide in a ratio of 1:3), a biological enzyme emulsifier (fatty acid glyceride and hexadecyltrimethylammonium chloride in a ratio of 1:1), and a biological enzyme stabilizer (sodium acetate and sodium citrate in a ratio of 1:3), and the mixing ratio is 2:1:1; The preparation method is the same as above.

[0025] The oil wells in Qinghai Oilfield onshore were unblocked. The reservoir temperature was 85°C and the mineralization was about 200000 mg / L. The high-temperature bio-enzyme unblocking agent prepared in Example 2 was used to unblock and increase production. The high-temperature bio-enzyme unblocking agent was diluted with water to a 3% aqueous solution. A pump truck was quickly injected into the wells at a speed of 200-500 m 3 , clean water is used to replace the oil, and the well is shut in for 3 to 7 days. After the well is opened, the average daily liquid production increases by more than 1 times, and the daily oil production increases by more than 1 times. The effective period is more than 180 days. The average cumulative oil production increase of a single well is more than 280t, and the highest cumulative oil production increase of a single well during the effective period is 468t.

[0026] Example 3

[0027] A high-temperature bio-enzyme plugging remover of the present invention is composed of the following components in parts by mass: 10 parts of biological enzyme preparation, 35 parts of biological active agent (lipopeptide surfactant and phenyl octadecylaminopropyl dimethylaminoacetate in a ratio of 1:1), 20 parts of biological organic acid (acetic acid), and 35 parts of water; The bio-enzyme preparation is made of 11 parts of bio-complex enzyme, 10 parts of bio-enzyme protective agent and 79 parts of water; The biological compound enzyme is prepared by mixing protein compound enzyme, β-mannanase and lipase in a ratio of 1:1:1; The biological enzyme protective agent is a combination of a biological enzyme antioxidant (vitamin C and polyethylene glycol in a ratio of 1:2), a biological enzyme emulsifier (sucrose ester and stearic acid triethanolamine salt in a ratio of 2:1), and a biological enzyme stabilizer (sodium acetate and calcium sulfate in a ratio of 1:1), and the mixing ratio is 2:1:1; The preparation method is the same as above.

[0028] The water injection wells in the Daqing peripheral oil field on land were unblocked. The reservoir temperature was 50°C and the salinity was about 7800 mg / L. The high-temperature bio-enzyme unblocking agent prepared in Example 3 was used for unblocking and injection. The high-temperature bio-enzyme unblocking agent was diluted with water to a 5% aqueous solution by mass concentration. A pump truck was quickly injected into the wells at a depth of 50 to 150 m 3 , replace with clean water and shut down the well for 24 to 72 hours. The injection pressure is reduced by more than 30% on average, the daily injection volume is increased by several times, the average efficiency is more than 95%, and the effective period can be extended by more than 300 days.

[0029] Example 4

[0030] A high-temperature bio-enzyme plugging remover of the present invention is composed of the following components in parts by mass: 15 parts of biological enzyme preparation, 25 parts of biological active agent (phenyl octadecylaminopropyl dimethylaminoacetate), 25 parts of biological organic acid (acetic acid, formic acid, lactic acid in a ratio of 1:1:1), 35 parts of water; The bio-enzyme preparation is made of 16 parts of bio-complex enzyme, 20 parts of bio-enzyme protective agent and 64 parts of water; The biological complex enzyme is prepared from protein complex enzyme, xanthan gum lyase, β-mannanase, lipase and cellulase in a ratio of 1:2:2:1:1; The biological enzyme protective agent is a combination of a biological enzyme antioxidant (gelatin polysaccharide and trehalose in a ratio of 1:2), a biological enzyme emulsifier (cetyltrimethylammonium chloride and stearic acid triethanolamine salt in a ratio of 1:1), and a biological enzyme stabilizer (sodium citrate and potassium phosphate in a ratio of 1:3), and the ratio is 2:1:1; The preparation method is the same as above.

[0031] In the Qinghai oil field onshore water injection well unblocking, the reservoir temperature is 90°C, and the mineralization is about 50000 mg / L. The high temperature biological enzyme unblocking agent prepared in Example 4 is used for unblocking and injection. The high temperature biological enzyme unblocking agent is diluted with water to a 5% aqueous solution by mass concentration, and a pump truck is quickly injected into 50-150m 3 , replace with clean water and shut down the well for 24 to 72 hours. The injection pressure is reduced by more than 30% on average, the daily injection volume is increased by several times, the average efficiency is more than 92%, and the effective period can be extended by more than 300 days.

[0032] Example 5

[0033] A high-temperature bio-enzyme plugging remover of the present invention is composed of the following components in parts by mass: 17 parts of biological enzyme preparation, 40 parts of biological active agent (lipopeptide surfactant), 13 parts of biological organic acid (acetic acid), 30 parts of water; The bio-enzyme preparation is made of 15 parts of bio-complex enzyme, 17 parts of bio-enzyme protective agent and 68 parts of water; The biological complex enzyme is prepared by mixing protein complex enzyme, xanthan gum lyase, β-mannanase, lipase, cellulase and hemicellulase in a ratio of 1:1:1:1:1:1; The bio-enzyme protective agent is a combination of a bio-enzyme antioxidant (vitamin E and trehalose in a ratio of 1:1), a bio-enzyme emulsifier (fatty acid glyceride and stearic acid triethanolamine salt in a ratio of 1:1), and a bio-enzyme stabilizer (sodium acetate, potassium phosphate, and calcium sulfate in a ratio of 1:1:2), and the mixing ratio is 2:1:1; The preparation method is the same as above.

[0034] The oil well in Bohai Oilfield was unblocked. The reservoir temperature was 65°C and the mineralization was about 35000 mg / L. The high-temperature bio-enzyme unblocking agent prepared in Example 5 was used to unblock and increase production. The high-temperature bio-enzyme unblocking agent was diluted with water to an aqueous solution with a mass concentration of 2%. A pump truck was quickly injected into the well at a depth of 80 to 250 m 3, replace with clean water and shut down the well for 2 to 5 days. After the well is opened, the average daily liquid production will increase by more than 1 times, the daily oil production will increase by more than 1 times, the crude oil viscosity reduction rate can reach more than 98%, the effective period is more than 150 days, the average cumulative oil increase of a single well is more than 500t, and the highest cumulative oil increase of a single well during the effective period is 1,426t.

[0035] Example 6

[0036] A high-temperature bio-enzyme plugging remover of the present invention is composed of the following parts by mass: 29 parts of bio-enzyme preparation, 30 parts of bioactive agent (lipopeptide surfactant and phenyl octadecylaminopropyl dimethylaminoacetate in a ratio of 1:1), 15 parts of bio-organic acid (acetic acid, propionic acid, lactic acid in a ratio of 2:1:1), and 26 parts of water; The bio-enzyme preparation is made of 18 parts of bio-complex enzyme, 20 parts of bio-enzyme protective agent and 62 parts of water; The biological complex enzyme is prepared by mixing protein complex enzyme, xanthan gum lyase, β-mannanase, lipase, cellulase and hemicellulase in a ratio of 1:2:2:1:1:1; The bio-enzyme protective agent is a combination of a bio-enzyme antioxidant (vitamin E and trehalose in a ratio of 1:2), a bio-enzyme emulsifier (sucrose ester and hexadecyltrimethylammonium chloride in a ratio of 1:1), and a bio-enzyme stabilizer (sodium acetate, potassium phosphate, and calcium sulfate in a ratio of 1:2:2), and the mixing ratio is 2:1:1; The preparation method is the same as above.

[0037] The oil well in Bohai Oilfield was unblocked. The reservoir temperature was 95°C and the mineralization was about 40000 mg / L. The high-temperature bio-enzyme unblocking agent prepared in Example 6 was used to unblock and increase production. The high-temperature bio-enzyme unblocking agent was diluted with water to a 10% aqueous solution by mass concentration. A pump truck was quickly injected into the well at 80-250 m 3 , replace with clean water and shut down the well for 3 to 7 days. After the well is opened, the average daily liquid production will increase by more than 1 times, the daily oil production will increase by more than 1 times, the crude oil viscosity reduction rate can reach more than 98%, the effective period is more than 150 days, the average cumulative oil increase of a single well is more than 500t, and the highest cumulative oil increase of a single well during the effective period is 1,265t.

[0038] Example 7

[0039] A high-temperature bio-enzyme plugging remover of the present invention is composed of the following components in parts by mass: 15 parts of biological enzyme preparation, 37 parts of biological active agent (phenyl octadecylaminopropyl dimethylaminoacetate), 20 parts of biological organic acid (acetic acid, propionic acid, lactic acid in a ratio of 1:1:1), and 28 parts of water.

[0040] The bio-enzyme preparation is made of 16 parts of bio-complex enzyme, 16 parts of bio-enzyme protective agent and 68 parts of water; The biological complex enzyme is prepared by mixing protein complex enzyme, xanthan gum lyase, β-mannanase, lipase, cellulase and hemicellulase in a ratio of 1:1:1:1:1:1; The biological enzyme protective agent is a combination of a biological enzyme antioxidant (vitamin E and trehalose in a ratio of 1:2), a biological enzyme emulsifier (sucrose ester and hexadecyltrimethylammonium chloride in a ratio of 1:1), and a biological enzyme stabilizer (sodium acetate, potassium phosphate, and calcium sulfate in a ratio of 1:1:1), and the ratio is 2:1:1; The preparation method is the same as above.

[0041] The water injection well in Bohai Oilfield was unblocked. The reservoir temperature was 60°C and the salinity was about 26000 mg / L. The high-temperature bio-enzyme unblocking agent prepared in Example 7 was used for unblocking and injection. The high-temperature bio-enzyme unblocking agent was diluted with water to a 5% aqueous solution by mass concentration. A pump truck was quickly injected into the well at 20 to 130 m 3 , replace with clean water and shut down the well for 24 to 72 hours. The injection pressure is reduced by more than 30% on average, and the average efficiency is more than 90%.

[0042] Example 8

[0043] A high-temperature bio-enzyme plugging remover of the present invention is composed of the following components in parts by mass: 18 parts of biological enzyme preparation, 25 parts of biological active agent (the ratio of lipopeptide surfactant and phenyl octadecylaminopropyl dimethylaminoacetate is 1:1), 27 parts of biological organic acid (the ratio of acetic acid, propionic acid and lactic acid is 2:1:1), and 30 parts of water.

[0044] The bio-enzyme preparation is made of 17 parts of bio-complex enzyme, 18 parts of bio-enzyme protective agent and 65 parts of water; The biological complex enzyme is prepared from protein complex enzyme, xanthan gum lyase, β-mannanase, lipase, cellulase and hemicellulase in a ratio of 1:2:2:1:1:1; The biological enzyme protective agent is a combination of a biological enzyme antioxidant (vitamin E and trehalose in a ratio of 1:2), a biological enzyme emulsifier (sucrose ester and hexadecyltrimethylammonium chloride in a ratio of 1:1), and a biological enzyme stabilizer (sodium acetate, potassium phosphate, and calcium sulfate in a ratio of 1:2:3), and the ratio is 2:1:1; The preparation method is the same as above.

[0045] The water injection well in Bohai Oilfield was unblocked. The reservoir temperature was 80°C and the salinity was about 20000 mg / L. The high-temperature bio-enzyme unblocking agent prepared in Example 8 was used for unblocking and injection. The high-temperature bio-enzyme unblocking agent was diluted with water to a 5% aqueous solution. A pump truck was quickly injected into the well at 20-130 m 3, replace with clean water and shut down the well for 24 to 72 hours. The injection pressure is reduced by more than 30% on average, and the average efficiency is more than 90%.

[0046] Comparative Example 1 The existing bio-enzyme plugging remover is composed of the following parts by mass: 10 parts of biological enzyme preparation, 40 parts of biological active agent (lipopeptide surfactant), 10 parts of biological organic acid (acetic acid), 40 parts of water; The bio-enzyme preparation is made of 10 parts of bio-complex enzyme and 90 parts of water; The biological complex enzyme is prepared by mixing protein complex enzyme, xanthan gum lyase, beta-mannanase and lipase in a ratio of 1:1:1:1.

[0047] Comparative Example 2 The existing bio-enzyme plugging remover is composed of the following parts by mass: 18 parts of biological complex enzyme, 25 parts of biological active agent (the ratio of lipopeptide surfactant and phenyl octadecylaminopropyl dimethylaminoacetate is 1:1), 27 parts of biological organic acid (the ratio of acetic acid, propionic acid and lactic acid is 2:1:1), and 30 parts of water.

[0048] The bio-enzyme preparation is made of 17 parts of bio-complex enzyme and 83 parts of water; The biological compound enzyme is prepared from protein compound enzyme, xanthan gum lyase, lipase and cellulase in a ratio of 1:2:2:1.

[0049] Performance Testing The high-temperature bio-enzyme plugging removers prepared in Examples 1 to 8 and Comparative Examples 1-2 were subjected to performance tests (including salt resistance, temperature resistance, residual rubber degradation rate, organic matter plugging removal rate, and core plugging removal rate); Experimental conditions: Salt resistance: First prepare simulated formation water with a specified mineralization, then use the prepared formation water to dilute the bio-enzyme plugging agent to the use concentration, and then conduct other performance tests; Temperature resistance: After the biological enzyme blockage is heated to the specified temperature, it is placed for 24 hours before the performance test is carried out; Residual rubber degradation rate: Take residual rubber samples from the oil field and measure the degradation time and degradation rate of the bio-enzyme plugging agent; Organic matter degradation rate: Take samples on site (including organic scale samples such as colloid, asphalt, wax, etc.) and measure the organic matter degradation rate of the bio-enzyme plugging agent; Core unblocking rate: prepare rectangular cores and simulated scale samples (containing residual glue, organic matter, and inorganic scale in a ratio of 3:5:1), block the cores and leave them for more than 48 hours, then use bio-enzyme unblocking agent to unblock them and measure the core unblocking rate.

[0050] The test results are shown in Table 1:

[0051] It can be seen from the test results in Table 1 that the high-temperature bio-enzyme plugging remover prepared in the embodiment of the present invention can solve the blockage problems (residual glue, organic matter deposition, inorganic scale, etc.) of water injection wells and production wells in onshore or offshore oil fields. Compared with the conventional bio-enzyme plugging remover system, it has a high salt tolerance of up to 200,000 mg / L; a wide temperature range with a maximum temperature of up to 100°C; a residual glue degradation rate of >98%, up to 99.5%; an organic matter degradation rate of >97%, up to 99.06%; a core plugging removal rate of >97%, up to 99.25%, reflecting excellent performance.

[0052] It can be seen from the above embodiments that the high-temperature bio-enzyme plugging remover provided by the present invention has a good effect of removing blockages and increasing oil production at the oil well end, and at the same time has a good effect of reducing pressure and increasing injection at the water well end. It is better than the current conventional plugging removal technology, and is better than other bio-enzyme plugging removers on the market in terms of high temperature resistance and mineralization resistance. The cost can be saved by at least 25% compared with other bio-enzyme plugging removers.

[0053] The above embodiments are only used to help understand the method and core idea of ​​the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made to the present invention without departing from the principles of the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A high temperature bio-enzyme plugging remover, characterized in that The invention is composed of the following ingredients in parts by weight: 1 to 20 parts of biological enzyme preparation, 1 to 40 parts of biological active agent, 1 to 40 parts of biological organic acid, and 0 to 97 parts of water; The biological enzyme preparation is composed of the following components in parts by weight: 1-20 parts of biological complex enzyme, 1-20 parts of biological enzyme protective agent and 60-98 parts of water.

2. A high temperature bio-enzyme plugging remover according to claim 1, characterized in that: The biological complex enzyme is composed of two or more of protein complex enzyme, cellulase, hemicellulase, xanthan gum lyase, beta-mannanase and lipase mixed in any proportion.

3. A high temperature bio-enzyme plugging remover according to claim 1, characterized in that: The biological enzyme protective agent is a biological enzyme antioxidant, a biological enzyme emulsifier, and a biological enzyme stabilizer, and the combined weight ratio is 2:1:

1.

4. A high temperature bio-enzyme plugging remover as claimed in claim 3, characterized in that: The biological enzyme antioxidant is composed of one or more of vitamin C, vitamin E, trehalose, gelatin polysaccharide and polyethylene glycol mixed in any proportion.

5. A high temperature bio-enzyme plugging remover as claimed in claim 3, characterized in that: The biological enzyme emulsifier is composed of one or more of sucrose ester, fatty acid glyceride, hexadecyltrimethylammonium chloride, stearic acid triethanolamine salt and benzalkonium bromide mixed in any proportion.

6. A high temperature bio-enzyme plugging remover as claimed in claim 3, characterized in that: The biological enzyme stabilizer is composed of one or more of sodium acetate, sodium citrate, sodium nitrate, calcium sulfate and potassium phosphate mixed in any proportion.

7. A high temperature bio-enzyme plugging remover according to claim 1, characterized in that: The bioactive agent is composed of one of lipopeptide surfactant and phenyl octadecylaminopropyl dimethylaminoacetate or a mixture of the two in any proportion.

8. A high temperature bio-enzyme plugging remover according to claim 1, characterized in that: The biological organic acid is composed of one or more of lactic acid, formic acid, acetic acid, propionic acid, oxalic acid and benzoic acid mixed in any proportion.

9. An application of a high temperature bio-enzyme plugging remover, characterized in that: The high-temperature bio-enzyme plugging remover has a high salt tolerance of up to 200,000 mg / L and is suitable for oil reservoir temperatures ranging from 20 to 100°C.

10. An application of a high temperature bio-enzyme plugging remover, characterized in that: It is suitable for unblocking water injection wells and oil production wells on land and offshore.

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