Stem cell culture device and method thereof

Abstract

The invention relates to the technical field of stem cell culture, in particular to a stem cell culture device and a method thereof.The stem cell culture device mainly comprises a culture box, a door plate is movably installed on the side face of the culture box in a penetrating mode, a supporting shaft is rotatably installed on the inner wall of the lower end of the culture box through a damping bearing, and a placing plate and a dosing mechanism are fixedly installed at the upper end of the supporting shaft; the dosing mechanism is arranged above the placing plate, and the rotating mechanism is located in the incubator. An electric push rod is started to drive a disc to move upwards, and a piston rod and a piston are driven to move upwards, so that a conveying cylinder absorbs a culture solution from a storage box through a connecting pipe, the electric push rod is started to drive the lower piston to move downwards, the piston pushes the culture solution in the conveying cylinder to be conveyed into a culture dish from a liquid adding head through a conveying pipe, and a door plate does not need to be opened in the midway. Bacteria are prevented from entering a culture dish in culture, and stem cells in the culture dish are not easily polluted by external bacteria, so that the survival rate of the stem cells is ensured, and the culture quality of the stem cells is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell culture, in particular to a stem cell culture device and method. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into multiple functional cells. According to the developmental stage of stem cells, they are divided into embryonic stem cells and adult stem cells. Stem cells are a type of undifferentiated, immature cells that have the potential to regenerate various tissues, organs and the human body. They are called "universal cells" in the medical field. Therefore, the cultivation of stem cells is particularly important.

[0003] The Chinese patent publication number CN211947079U discloses a stem cell culture device, including a bottom plate. The stem cell culture device is provided with small squares, a microscope camera and a display, so that culture dishes can be placed in multiple small squares, and the microscope camera can display the stem cell culture status in each different culture dish on the display in real time, so that the stem cell culture device not only has high culture efficiency, but also is easy to operate, and effectively avoids sample contamination caused by frequent opening and closing of the door.

[0004] However, when the device is adding medicine, the movable door needs to be opened and the nutrient solution needs to be added to the culture dish. Since the movable door is opened multiple times, it is easy for external bacteria to enter the culture dish. The stem cells in the culture dish are easily contaminated by external bacteria, resulting in a decrease in the survival rate of the stem cells and a reduction in the quality of the culture. Summary of the invention

[0005] The object of the present invention is to provide a stem cell culture device and method thereof to solve the problems raised by the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The stem cell culture device comprises: a culture box, a door panel is movably installed on the side of the culture box, a support shaft is rotatably installed on the inner wall of the lower end of the culture box through a damping bearing, a placement plate is fixedly installed on the upper end of the support shaft, a plurality of fixed grooves are opened on the top surface of the placement plate, and irradiation lamps are fixedly installed on the inner walls of both sides of the culture box respectively;

[0008] The dosing mechanism is arranged above the placement plate, and includes a delivery cylinder located above the incubator, a delivery pipe is fixedly installed through the bottom of the delivery cylinder, and a liquid adding head is fixedly installed at the lower end of the delivery pipe. The dosing mechanism is used to add medicine to the culture dish;

[0009] The rotating mechanism is located inside the incubator, and includes a fixed column fixedly mounted on the top surface of the placement plate, and a cylinder is fixedly mounted on the inner wall of the upper end of the incubator, and the rotating mechanism is used to rotate the placement plate;

[0010] The fixing mechanism is arranged inside the placement plate. The fixing mechanism comprises an arc-shaped plate located inside the placement plate. A pull rod is fixedly installed on the side of the arc-shaped plate. The fixing mechanism is used to fix the culture dish.

[0011] Preferably, a storage box is fixedly installed on the top surface of the incubator, a feed port is provided on the top surface of the storage box, a transparent plate is fixedly installed on the side of the storage box, a plurality of scale lines are fixedly installed on the side of the storage box, support legs are fixedly installed on the top surface of the incubator, and a round frame is fixedly installed on the upper end of the support legs.

[0012] Preferably, the top surface of the circular frame is fixedly connected to the bottom surface of the conveying cylinder, a piston is slidably mounted on the inner wall of the conveying cylinder, a piston rod is fixedly mounted on the top surface of the piston, the upper end of the piston rod slides through the inner wall of the upper end of the conveying cylinder and extends to the top of the conveying cylinder, and a disc is fixedly mounted on the upper end of the piston rod.

[0013] Preferably, two supporting columns are fixedly installed on the top surface of the circular frame, a circular plate is fixedly installed on the upper ends of the two supporting columns, an electric push rod is fixedly installed on the top surface of the circular plate, the lower end of the electric push rod slides through the top surface of the circular plate and is fixedly connected to the top surface of the disc, two limit sleeves are fixedly installed on the outer wall of the disc, and the inner walls of the two limit sleeves are slidably connected to the outer walls of the two supporting columns respectively.

[0014] Preferably, the lower end of the delivery pipe is fixedly passed through the top surface of the incubator and extends to the inside of the incubator, a connecting pipe is fixedly installed on the outer wall of the delivery pipe, and the other end of the connecting pipe is fixedly passed through the side of the storage box and extends to the inside of the storage box, and one-way valve 1 and one-way valve 2 are respectively installed on the connecting pipe and the delivery pipe.

[0015] Preferably, the outer wall of the cylinder is provided with two slots, the outer wall of the fixed column is provided with six vertical slots and six oblique slots, the six oblique slots sequentially connect the six vertical slots end to end, and the outer wall of the disc is fixedly installed with two connecting plates.

[0016] Preferably, push rods are fixedly installed on the bottom surfaces of the two connecting plates, and the lower ends of the two push rods slide through the top surface of the round frame and the top surface of the incubator to extend into the interior of the incubator. Push blocks are fixedly installed on the outer walls of the lower ends of the two push rods, and the outer walls of the two push blocks are slidably connected to the inner wall of the trough body, the inner wall of the vertical groove and the inner wall of the inclined groove.

[0017] Preferably, the inner walls of one side of the multiple vertical grooves are respectively provided with adjustment grooves, and the inner walls of the multiple adjustment grooves are respectively rotatably installed with baffles, the outer walls on the other side of the multiple baffles abut against the inner wall of the fixed column on one side, the bottom surface of the baffle is elastically connected to the inner wall of the adjustment groove through an elastic member, and the side surface of the baffle is slidably connected to the outer wall of the push block.

[0018] Preferably, six fixed grooves and six arc-shaped plates are provided, and movable grooves are respectively opened on the inner walls of the six fixed grooves. The inner walls of the six movable grooves are respectively slidably connected to the side surfaces of the six arc-shaped plates. Multiple pull rods are provided, and one end of the multiple pull rods slides through the inner walls of the multiple movable grooves and extends to the outside of the placement plate. Pull plates are respectively fixedly installed on one end of the multiple pull rods, and elastic parts two are respectively slidably installed on the outer walls of the multiple pull rods, and the two ends of the multiple elastic parts two are respectively fixedly connected to the side surfaces of the multiple arc-shaped plates and the inner walls of the multiple movable grooves.

[0019] The stem cell culture method comprises the following steps:

[0020] S1: Open the door panel, place the culture dish carrying the stem cells in the fixing groove, fix the culture dish by the arc plate (402), and close the door panel;

[0021] S2: After the culture solution is introduced into the culture dish through the delivery tube, it is then delivered from the liquid adding head to the culture dish through the delivery tube;

[0022] S3: After one culture dish is added, the fixed column drives the culture dish on the placement plate to rotate 60 degrees, and the culture solution is added to the next culture dish;

[0023] S4: After the liquid is added, the device is placed for culture

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The present invention starts an electric push rod, which drives the disc to move upward, drives the piston rod and the piston to move upward, so that the delivery cylinder absorbs culture fluid from the storage box through the connecting pipe, starts the electric push rod to drive the lower piston to move downward, and the piston pushes the culture fluid in the delivery cylinder to be delivered from the liquid adding head to the culture dish through the delivery pipe. There is no need to open the door panel in the middle, so bacteria are prevented from entering the culture dish in culture. The stem cells in the culture dish are not easily contaminated by external bacteria, so the survival rate of the stem cells is guaranteed and the culture quality of the stem cells is improved.

[0026] When the disc moves downward, it drives the push rod and the push block to move downward. The push block moves on the inner wall of the vertical slot and drives the baffle plate to rotate to squeeze the elastic member. When the push block moves to the bottom of the vertical slot, after the drug addition in a culture dish is completed, the disc drives the push rod and the push block to move upward. Under the blockage of the baffle plate, the push block moves in the inclined slot. When the push block moves into the slot body, the fixed column and the placement plate rotate 60 degrees, and the next culture dish rotates to the bottom of the liquid adding head. Repeat the operation to complete the automatic liquid addition. There is no need to open the door panel and manually rotate the placement plate to adjust the position of the culture dish, thereby further preventing the stem cells in the culture dish from being contaminated.

[0027] By setting the movable groove, arc plate, pull rod, pull plate and elastic member 2, the elastic member 2 pushes the arc plate to move and applies thrust to the arc plate. Under the action of the arc plate and the fixed groove, the culture dish is fixed, making it difficult for the culture dish to shake and deviate, thereby improving the stability of stem cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0029] Figure 2 It is a schematic cross-sectional diagram of the three-dimensional structure of the incubator of the present invention;

[0030] Figure 3 It is a schematic cross-sectional view of the three-dimensional structure of the conveying tube of the present invention;

[0031] Figure 4 It is a schematic diagram of the three-dimensional structure of the liquid adding head of the present invention;

[0032] Figure 5 It is a schematic diagram of the three-dimensional structure of the fixing column of the present invention;

[0033] Figure 6 For the present invention Figure 5 Enlarged view of point A in the middle;

[0034] Figure 7 It is a cross-sectional schematic diagram of the three-dimensional structure of the placement plate of the present invention;

[0035] Figure 8 For the present invention Figure 7 Enlarged view of point B in the middle.

[0036] In the figure:

[0037] 1. Incubator; 101. Door panel; 102. Support shaft; 103. Damping bearing; 104. Placement plate; 105. Fixing slot; 106. Irradiation lamp;

[0038] 2. Dosing mechanism; 201. Storage box; 202. Feeding port; 203. Transparent plate; 204. Scale line; 205. Support leg; 206. Round frame; 207. Delivery cylinder; 208. Piston; 209. Piston rod; 210. Disc; 211. Support column; 212. Round plate; 213. Electric push rod; 214. Delivery pipe; 215. Dosing head; 216. Connecting pipe; 217. One-way valve 1; 218. One-way valve 2; 219. Limit sleeve;

[0039] 3. Rotating mechanism; 301. Cylinder; 302. Fixed column; 303. Slot body; 304. Vertical slot; 305. Oblique slot; 306. Connecting plate; 307. Push rod; 308. Push block; 309. Adjusting slot; 310. Elastic member 1; 311. Baffle;

[0040] 4. Fixing mechanism; 401. Moving groove; 402. Arc plate; 403. Pull rod; 404. Pull plate; 405. Elastic member 2. DETAILED DESCRIPTION

[0041] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0042] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present application.

[0043] like Figure 1-Figure 8 As shown, the present application provides a stem cell culture device, comprising: a culture box 1, a door panel 101 is movably installed on the side of the culture box 1, a support shaft 102 is rotatably installed on the inner wall of the lower end of the culture box 1 through a damping bearing 103, a placement plate 104 is fixedly installed on the upper end of the support shaft 102, a plurality of fixing grooves 105 are opened on the top surface of the placement plate 104, and irradiation lamps 106 are fixedly installed on the inner walls of both sides of the culture box 1;

[0044] The dosing mechanism 2 is arranged above the placement plate 104. The dosing mechanism 2 includes a delivery cylinder 207 located above the incubator 1. A delivery tube 214 is fixedly installed through the bottom surface of the delivery cylinder 207. A liquid adding head 215 is fixedly installed at the lower end of the delivery tube 214. The dosing mechanism 2 is used to add drugs to the culture dish;

[0045] Specifically, Figure 1-Figure 4 As shown, a storage box 201 is fixedly installed on the top surface of the incubator 1, a feed port 202 is provided on the top surface of the storage box 201, a transparent plate 203 is fixedly installed through the side of the storage box 201, a plurality of scale lines 204 are fixedly installed on the side of the storage box 201, a supporting leg 205 is fixedly installed on the top surface of the incubator 1, and a round frame 206 is fixedly installed on the upper end of the supporting leg 205.

[0046] In this embodiment, the culture medium is stored in a storage box 201 , and a transparent plate 203 and scale lines 204 are provided. The position of the culture medium in the storage box 201 can be observed through the transparent plate 203 , and the specific content of the culture medium can be observed through the scale lines 204 .

[0047] Specifically, Figure 1-Figure 4As shown, the top surface of the circular frame 206 is fixedly connected to the bottom surface of the conveying cylinder 207, a piston 208 is slidably mounted on the inner wall of the conveying cylinder 207, a piston rod 209 is fixedly mounted on the top surface of the piston 208, the upper end of the piston rod 209 slides through the inner wall of the upper end of the conveying cylinder 207 and extends to the top of the conveying cylinder 207, and a disc 210 is fixedly mounted on the upper end of the piston rod 209.

[0048] In this embodiment, the piston 208 is arranged to move on the inner wall of the delivery cylinder 207, so that liquid can be sucked and discharged.

[0049] Specifically, Figure 1-Figure 4 As shown, two support columns 211 are fixedly installed on the top surface of the circular frame 206, a circular plate 212 is fixedly installed on the upper ends of the two support columns 211, an electric push rod 213 is fixedly installed on the top surface of the circular plate 212, the lower end of the electric push rod 213 slides through the top surface of the circular plate 212 and is fixedly connected to the top surface of the disk 210, two limit sleeves 219 are fixedly installed on the outer wall of the disk 210, and the inner walls of the two limit sleeves 219 are slidably connected to the outer walls of the two support columns 211 respectively.

[0050] In this embodiment, the electric push rod 213 is provided to drive the circular plate 212 to move up and down, and the circular plate 212 drives the disc 210 to move, thereby driving the piston rod 209 and the piston 208 to move on the inner wall of the conveying cylinder 207.

[0051] Specifically, Figure 1-Figure 4 As shown, the lower end of the delivery pipe 214 is fixedly passed through the top surface of the incubator 1 and extends to the interior of the incubator 1, a connecting pipe 216 is fixedly installed on the outer wall of the delivery pipe 214, and the other end of the connecting pipe 216 is fixedly passed through the side of the storage box 201 and extends to the interior of the storage box 201, and a one-way valve 1 217 and a one-way valve 218 are respectively installed on the connecting pipe 216 and the delivery pipe 214.

[0052] In this embodiment: liquid is added to the stem cells in the culture dish through the provided delivery tube 214 and the liquid adding head 215, and a one-way valve 1 217 and a one-way valve 218 are respectively installed on the connecting tube 216 and the delivery tube 214, so that the delivery tube 207 can only absorb the culture fluid from the connecting tube 216 and the one-way valve 1 217, and discharge the culture fluid from the delivery tube 214 and the one-way valve 2 218.

[0053] The rotating mechanism 3 is located inside the incubator 1. The rotating mechanism 3 includes a fixed column 302 fixedly mounted on the top surface of the placement plate 104. A cylinder 301 is fixedly mounted on the inner wall of the upper end of the incubator 1. The rotating mechanism 3 is used to rotate the placement plate 104.

[0054] Specifically, Figure 1-Figure 6As shown, the outer wall of the cylinder 301 is provided with two slots 303, the outer wall of the fixed column 302 is provided with six vertical slots 304 and six inclined slots 305, the six inclined slots 305 sequentially connect the six vertical slots 304 from top to bottom, and two connecting plates 306 are fixedly installed on the outer wall of the disk 210.

[0055] In this embodiment, the push block 308 moves between the inclined slot 305 and the vertical slot 304 by means of the provided inclined slot 305 and the vertical slot 304 .

[0056] Specifically, Figure 1-Figure 6 As shown, push rods 307 are fixedly installed on the bottom surfaces of the two connecting plates 306, and the lower ends of the two push rods 307 slide through the top surface of the round frame 206 and the top surface of the incubator 1 to extend into the interior of the incubator 1. Push blocks 308 are fixedly installed on the outer walls of the lower ends of the two push rods 307, and the outer walls of the two push blocks 308 are slidably connected to the inner wall of the groove body 303, the inner wall of the vertical groove 304 and the inner wall of the inclined groove 305.

[0057] In this embodiment, the push block 308 is provided, and when the push rod 307 moves, the push block 308 moves internally, and the push block 308 moves upward and acts together with the inclined slot 305, driving the fixed column 302 and the placement plate 104 to rotate 60 degrees.

[0058] Specifically, Figure 1-Figure 6 As shown, the inner walls of one side of the multiple vertical grooves 304 are respectively provided with adjustment grooves 309, and the inner walls of the multiple adjustment grooves 309 are respectively rotatably installed with baffles 311, and the outer walls of the other sides of the multiple baffles 311 abut against the inner wall of the fixed column 302 on one side, and the bottom surface of the baffle 311 is elastically connected to the inner wall of the adjustment groove 309 through an elastic member 310, and the side surface of the baffle 311 is slidably connected to the outer wall of the push block 308.

[0059] In this embodiment, by providing a baffle 311 and an elastic member 310 , the push block 308 pushes the baffle 311 to move to the bottom of the vertical slot 304 . When moving upward, the push block 308 moves on the inner wall of the inclined slot 305 due to the obstruction of the baffle 311 .

[0060] The fixing mechanism 4 is arranged inside the placement plate 104. The fixing mechanism 4 includes an arc-shaped plate 402 located inside the placement plate 104. A pull rod 403 is fixedly installed on the side of the arc-shaped plate 402. The fixing mechanism 4 is used to fix the culture dish.

[0061] Specifically, Figure 1-Figure 8As shown, there are six fixed grooves 105 and six arc-shaped plates 402, and the inner walls of the six fixed grooves 105 are respectively provided with movable grooves 401. The inner walls of the six movable grooves 401 are respectively slidably connected to the side surfaces of the six arc-shaped plates 402. There are multiple pull rods 403, and one end of the multiple pull rods 403 slides through the inner walls of the multiple movable grooves 401 and extends to the outside of the placement plate 104. The pull plates 404 are respectively fixedly installed at one end of the multiple pull rods 403, and the outer walls of the multiple pull rods 403 are respectively slidably installed with elastic parts 405, and the two ends of the multiple elastic parts 405 are respectively fixedly connected to the side surfaces of the multiple arc-shaped plates 402 and the inner walls of the multiple movable grooves 401.

[0062] In this embodiment: by setting the elastic member 405 and the arc plate 402, the elastic member 405 pushes the arc plate 402 to move, applies thrust to the arc plate 402, and under the action of the arc plate 402 and the fixing groove 105, the culture dish is fixed, making it difficult for the culture dish to shake and deviate, thereby improving the stability of stem cell culture.

[0063] The stem cell culture method comprises the following steps:

[0064] S1: Open the door panel 101, place the culture dish carrying stem cells in the fixing groove 105, fix the culture dish by the arc plate 402, and close the door panel 101;

[0065] S2: After the culture solution is introduced into the culture dish through the delivery tube 207, it is then delivered to the culture dish from the liquid adding head 215 through the delivery tube 214;

[0066] S3: After one culture dish is added, the fixed column 302 drives the culture dish on the placement plate 104 to rotate 60 degrees, and the culture solution is added to the next culture dish;

[0067] S4: After the liquid is added, the device is placed for culture

[0068] The present solution is specifically as follows: by setting the moving groove 401, the arc plate 402, the pull rod 403, the pull plate 404 and the elastic member 405, the elastic member 405 pushes the arc plate 402 to move, applies thrust to the arc plate 402, and fixes the culture dish under the action of the arc plate 402 and the fixing groove 105, so that the culture dish is not easy to shake and deflect, and the stability of stem cell culture is improved, the electric push rod 213 is turned on, the electric push rod 213 drives the disc 210 to move up, drives the piston rod 209 and the piston 208 to move up, so that the conveying cylinder 207 absorbs the culture liquid from the storage box 201 through the connecting pipe 216, starts the electric push rod 213 to drive the lower piston 208 to move down, and the piston 208 pushes the culture liquid in the conveying cylinder 207 to be conveyed to the culture dish from the liquid adding head 215 through the conveying pipe 214, and there is no need to open the door panel 101 in the middle, so as to prevent bacteria from entering the culture dish in culture, and the stem cells in the culture dish are prevented from entering the culture dish in culture. The cells are not easily contaminated by external bacteria, thereby ensuring the survival rate of stem cells and improving the culture quality of stem cells. When the disc 210 moves downward, it drives the push rod 307 and the push block 308 to move downward. The push block 308 moves on the inner wall of the vertical groove 304 and drives the baffle 311 to rotate and squeeze the elastic member 310. When the push block 308 moves to the bottom of the vertical groove 304, after the drug addition in a culture dish is completed, the disc 210 drives the push rod 307 and the push block 308 to move upward. Under the obstruction of the baffle 311, the push block 308 moves into the inclined groove 305. When the push block 308 moves into the groove body 303, the fixed column 302 and the placement plate 104 rotate 60 degrees, and the next culture dish rotates to the bottom of the liquid adding head 215. Repeat the operation to complete the automatic liquid addition. There is no need to open the door panel 101 and manually rotate the placement plate 104 to adjust the position of the culture dish, thereby further preventing the stem cells in the culture dish from being contaminated.

[0069] A person skilled in the art should understand that the discussion of any of the above embodiments is only exemplary; within the scope of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0070] The present invention is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A stem cell culture device, comprising: An incubator (1), wherein a door panel (101) is movably installed on the side of the incubator (1), a support shaft (102) is rotatably installed on the inner wall of the lower end of the incubator (1) through a damping bearing (103), a placement plate (104) is fixedly installed on the upper end of the support shaft (102), and a plurality of fixing grooves (105) are provided on the top surface of the placement plate (104), and irradiation lamps (106) are fixedly installed on the inner walls on both sides of the incubator (1), characterized in that: A drug adding mechanism (2), the drug adding mechanism (2) being arranged above the placement plate (104), the drug adding mechanism (2) comprising a delivery cylinder (207) located above the incubator (1), a delivery tube (214) being fixedly installed through the bottom surface of the delivery cylinder (207), a liquid adding head (215) being fixedly installed at the lower end of the delivery tube (214), and the drug adding mechanism (2) being used for adding drugs to the culture dish; A rotating mechanism (3), the rotating mechanism (3) being located inside the incubator (1), the rotating mechanism (3) comprising a fixing column (302) fixedly mounted on the top surface of the placement plate (104), a cylinder (301) being fixedly mounted on the inner wall of the upper end of the incubator (1), and the rotating mechanism (3) being used to rotate the placement plate (104); A fixing mechanism (4), wherein the fixing mechanism (4) is arranged inside the placement plate (104), the fixing mechanism (4) comprises an arc-shaped plate (402) located inside the placement plate (104), a pull rod (403) is fixedly installed on the side of the arc-shaped plate (402), and the fixing mechanism (4) is used to fix the culture dish.

2. The stem cell culture device according to claim 1, characterized in that: A storage box (201) is fixedly mounted on the top surface of the incubator (1), a feed port (202) is provided on the top surface of the storage box (201), a transparent plate (203) is fixedly mounted through the side of the storage box (201), a plurality of scale lines (204) are fixedly mounted on the side of the storage box (201), a support leg (205) is fixedly mounted on the top surface of the incubator (1), and a round frame (206) is fixedly mounted on the upper end of the support leg (205).

3. The stem cell culture device according to claim 2, characterized in that: The top surface of the circular frame (206) is fixedly connected to the bottom surface of the conveying cylinder (207); a piston (208) is slidably mounted on the inner wall of the conveying cylinder (207); a piston rod (209) is fixedly mounted on the top surface of the piston (208); the upper end of the piston rod (209) slides through the inner wall of the upper end of the conveying cylinder (207) and extends to the top of the conveying cylinder (207); a disc (210) is fixedly mounted on the upper end of the piston rod (209).

4. The stem cell culture device according to claim 3, characterized in that: Two support columns (211) are fixedly mounted on the top surface of the circular frame (206); a circular plate (212) is fixedly mounted on the upper ends of the two support columns (211); an electric push rod (213) is fixedly mounted on the top surface of the circular plate (212); the lower end of the electric push rod (213) slides through the top surface of the circular plate (212) and is fixedly connected to the top surface of the circular disk (210); two limiting sleeves (219) are fixedly mounted on the outer wall of the circular disk (210); the inner walls of the two limiting sleeves (219) are respectively slidably connected to the outer walls of the two support columns (211).

5. The stem cell culture device according to claim 4, characterized in that: The lower end of the delivery pipe (214) is fixedly passed through the top surface of the incubator (1) and extends into the interior of the incubator (1); a connecting pipe (216) is fixedly passed through the outer wall of the delivery pipe (214); the other end of the connecting pipe (216) is fixedly passed through the side of the storage box (201) and extends into the interior of the storage box (201); a one-way valve (217) and a one-way valve (218) are respectively installed on the connecting pipe (216) and the delivery pipe (214).

6. The stem cell culture device according to claim 3, characterized in that: The outer wall of the cylinder (301) is provided with two grooves (303), the outer wall of the fixed column (302) is provided with six vertical grooves (304) and six inclined grooves (305), the six inclined grooves (305) sequentially connect the six vertical grooves (304) end to end, and the outer wall of the disk (210) is fixedly mounted with two connecting plates (306).

7. The stem cell culture device according to claim 6, characterized in that: Push rods (307) are fixedly mounted on the bottom surfaces of the two connecting plates (306), and the lower ends of the two push rods (307) slide through the top surface of the round frame (206) and the top surface of the incubator (1) to extend into the interior of the incubator (1). Push blocks (308) are fixedly mounted on the outer walls of the lower ends of the two push rods (307), and the outer walls of the two push blocks (308) are slidably connected to the inner wall of the trough body (303), the inner wall of the vertical groove (304), and the inner wall of the inclined groove (305).

8. The stem cell culture device according to claim 7, characterized in that: An adjusting groove (309) is respectively formed on the inner wall of one side of the plurality of vertical grooves (304), and a baffle (311) is rotatably mounted on the inner wall of the plurality of adjusting grooves (309). The outer wall of the other side of the plurality of baffles (311) abuts against the inner wall of the fixed column (302) on one side. The bottom surface of the baffle (311) is elastically connected to the inner wall of the adjusting groove (309) through an elastic member 1 (310), and the side surface of the baffle (311) is slidably connected to the outer wall of the push block (308).

9. The stem cell culture device according to claim 1, characterized in that: There are six fixed grooves (105) and six arc-shaped plates (402), and the inner walls of the six fixed grooves (105) are respectively provided with movable grooves (401). The inner walls of the six movable grooves (401) are respectively slidably connected to the side surfaces of the six arc-shaped plates (402). There are multiple pull rods (403), and one end of the multiple pull rods (403) slides through the inner walls of the multiple movable grooves (401) and extends to the outside of the placement plate (104). One end of the multiple pull rods (403) is respectively fixedly installed with a pull plate (404), and the outer walls of the multiple pull rods (403) are respectively slidably installed with elastic parts 2 (405), and the two ends of the multiple elastic parts 2 (405) are respectively fixedly connected to the side surfaces of the multiple arc-shaped plates (402) and the inner walls of the multiple movable grooves (401).

10. A stem cell culture method, applicable to the stem cell culture device according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1: Open the door panel (101), place the culture dish carrying the stem cells in the fixing groove (105), fix the culture dish by the arc plate (402), and close the door panel (101); S2: After the culture liquid is introduced through the delivery tube (207), it is then delivered to the culture dish from the liquid adding head 215 through the delivery tube (214); S3: After one culture dish is added, the fixed column (302) drives the culture dish on the placement plate (104) to rotate 60 degrees, and the culture solution is added to the next culture dish; S4: After the liquid is added, the device is placed for culture.

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