A device for synchronously detecting longitudinal modulus and shear modulus of biological tissue

Abstract

The present application discloses a device for synchronous detection of the longitudinal modulus and shear modulus of biological tissue, which relates to the technical field of elastic imaging of biological tissue to be measured. In the device, a three-dimensional displacement stage is arranged on the dual-angle input and output optical paths of a dual-angle Brillouin scattering signal generating and receiving component; an optical fiber coupler is arranged on the coupled output optical path of the dual-angle Brillouin scattering signal generating and receiving component; a measurement component and the optical fiber coupler are connected through an optical fiber; the three-dimensional displacement stage moves so that the dual-angle focusing point of the dual-angle Brillouin scattering signal generating and receiving component coincides with a target detection point; the dual-angle Brillouin scattering signal generating and receiving component excites and receives the Brillouin scattering signal at the target detection point; and the measurement component collects the Brillouin scattering signal. By setting up the dual-angle Brillouin scattering signal generating and receiving component, the present application can complete the synchronous detection of the longitudinal modulus spectrum and the shear modulus spectrum.

Description

Technical Field

[0001] The present application relates to the technical field of elastic imaging of biological tissue to be tested, and in particular to a device for synchronously detecting the longitudinal modulus and shear modulus of biological tissue. Background Art

[0002] The longitudinal modulus of the biological tissue being tested is not exactly the same as the Young's modulus; it is also related to the shear modulus and bulk modulus. The test sample of the biological tissue being tested differs from the conventional tensile modulus: as the biological tissue being tested is highly hydrated, due to the high incompressibility of water, the longitudinal modulus measured by Brillouin testing is closer to the bulk modulus. Therefore, a detection device is required to simultaneously obtain the shear modulus and longitudinal modulus of the biological tissue being tested. Using a high-resolution spectrometer to obtain spectra of the two moduli, the correlation between the shear modulus and the longitudinal modulus can be determined. Summary of the Invention

[0003] The purpose of this application is to provide a device for synchronous detection of the longitudinal modulus and shear modulus of biological tissue, which can complete the synchronous detection of the longitudinal modulus spectrum and the shear modulus spectrum.

[0004] To achieve the above objectives, this application provides the following solutions:

[0005] The present application provides a device for synchronous detection of longitudinal modulus and shear modulus of biological tissue, comprising: a three-dimensional translation stage, a dual-angle Brillouin scattering signal generating and receiving component, an optical fiber coupler, and a measurement component;

[0006] The three-dimensional translation stage is arranged on the dual-angle input and output optical paths of the dual-angle Brillouin scattering signal generating and receiving component; the optical fiber coupler is arranged on the coupled output optical path of the dual-angle Brillouin scattering signal generating and receiving component; the measurement component is connected to the optical fiber coupler via an optical fiber;

[0007] The three-dimensional translation stage is used to place the biological tissue to be tested; the three-dimensional translation stage is used to drive the biological tissue to be tested to move so that the dual-angle focusing point of the dual-angle Brillouin scattering signal generating and receiving component coincides with the target detection point;

[0008] The dual-angle Brillouin scattering signal generating and receiving component is used to emit laser light from two angles and focus on a target detection point on the biological tissue to be tested; the dual-angle Brillouin scattering signal generating and receiving component is also used to receive a Brillouin scattering signal at the target detection point; the Brillouin scattering signal includes backscattered light, first side scattered light, and second side scattered light; the Brillouin scattering signal is generated by the biological tissue to be tested at the target detection point after being irradiated by the laser light at two angles;

[0009] The optical fiber coupler is used to couple the backscattered light, the first sidescattered light and the second sidescattered light at the target detection point into a coupled light beam, and input the coupled light beam into the measurement component;

[0010] The measurement component is used to separate the coupled light beam into Brillouin scattering signals and collect the Brillouin scattering signals; the Brillouin scattering signals are used to express the longitudinal modulus and shear modulus at the target detection point.

[0011] Optionally, the dual-angle Brillouin scattering signal generating and receiving component includes: a laser emitting component, a polarization beam splitter, a first angle input and output optical path component, and a second angle input and output optical path component;

[0012] The polarization beam splitter is arranged on the output light path of the laser emitting assembly;

[0013] The first angle input and output optical path component is arranged on the first angle input and output optical path of the polarization beam splitter;

[0014] The second angle input and output optical path component is arranged on the second angle input and output optical path of the polarization beam splitter;

[0015] The laser emitting component is used to emit laser;

[0016] The polarization beam splitter is used to split the laser into a reflected light beam and a transmitted light beam;

[0017] The first angle input and output optical path component is used to focus the reflected light beam to the target detection point; the first angle input and output optical path is also used to obtain backscattered light and first side scattered light;

[0018] The second angle input and output optical path component is used to focus the transmitted light beam to the target detection point; the second angle input and output optical path is also used to obtain the second side scattered light;

[0019] The reflected output light path and the transmitted output light path of the polarization beam splitter are collinear; the reflected output light path and the transmitted output light path of the polarization beam splitter serve as coupled output light paths of the dual-angle Brillouin scattering signal generating and receiving component;

[0020] The reflected output light path of the polarization beam splitter is the reflected output light path of the second side scattered light through the polarization beam splitter; the transmitted output light path of the polarization beam splitter is the transmitted output light path of the backscattered light and the first side scattered light through the polarization beam splitter.

[0021] Optionally, the laser emission assembly includes: a laser, a first reflector and a half-wave plate;

[0022] The first reflector is arranged on the output optical path of the laser;

[0023] The half-wave plate is arranged on the output optical path of the first reflector;

[0024] The polarization beam splitter is arranged on the output light path of the half wave plate.

[0025] Optionally, the laser is a 532nm laser or a 780nm laser.

[0026] Optionally, the first angle input and output optical path components include: a quarter wave plate, a second reflector and a first focusing objective lens;

[0027] The quarter wave plate is arranged on the first angle input and output optical path of the polarization beam splitter;

[0028] The second reflector is arranged on the output optical path of the quarter-wave plate;

[0029] The first focusing objective lens is arranged on the output light path of the second reflecting mirror;

[0030] The three-dimensional translation stage is arranged on the output optical path of the first focusing objective lens.

[0031] Optionally, the second angle input and output optical path component includes: a third reflector and a second focusing objective lens;

[0032] The third reflector is arranged on the second angle input and output optical path of the polarization beam splitter;

[0033] The second focusing objective lens is arranged on the output light path of the third reflecting mirror;

[0034] The three-dimensional translation stage is arranged on the output optical path of the second focusing objective lens.

[0035] Optionally, the measurement component includes: a spectrometer and a signal collector;

[0036] The spectrometer is connected to the fiber coupler via an optical fiber; the spectrometer is used to separate the coupled light beam into Brillouin scattering signals;

[0037] The signal collector is arranged on the output optical path of the spectrometer; the signal collector is used to collect Brillouin scattering signals.

[0038] Optionally, the spectrometer includes: a collimator, a first cylindrical lens, a first virtual imaging phase array, a fourth reflector, a second cylindrical lens, a first lens, a fifth reflector, a second virtual imaging phase array, and a second lens;

[0039] The collimator is connected to the fiber coupler via an optical fiber;

[0040] The first cylindrical lens is arranged on the output light path of the collimator;

[0041] The first virtual imaging phase array is arranged on the output optical path of the first cylindrical lens;

[0042] The fourth reflector is arranged on the output optical path of the first virtual imaging phase array;

[0043] The second cylindrical lens is arranged on the output light path of the fourth reflector;

[0044] The first lens is arranged on the output light path of the second cylindrical lens;

[0045] The fifth reflector is arranged on the output light path of the first lens;

[0046] The second virtual imaging phase array is arranged on the output optical path of the fifth reflector;

[0047] The second lens is arranged on the output optical path of the second virtual imaging phase array;

[0048] The signal collector is arranged on the output optical path of the second lens.

[0049] Optionally, the signal collector is an electron multiplying CCD.

[0050] Optionally, the three-dimensional displacement platform is provided with a first bracket and a second bracket;

[0051] The first bracket is rotatably connected to the three-dimensional translation stage; the first bracket is used to place the first focusing objective lens;

[0052] The second bracket is rotatably connected to the three-dimensional translation stage; the second bracket is used to place the second focusing objective lens;

[0053] Rotating the first bracket and the second bracket can adjust the angle of the Brillouin scattering signal.

[0054] According to the specific embodiments provided in this application, this application discloses the following technical effects:

[0055] The present application provides a device for synchronous detection of the longitudinal modulus and shear modulus of biological tissues. By measuring the dual-angle Brillouin scattering frequency shift of the biological tissue to be tested, the longitudinal wave velocity and the shear wave velocity are obtained, thereby realizing synchronous detection of the longitudinal modulus and shear modulus of the biological tissue to be tested. The device has a simple structure and can realize synchronous measurement of the longitudinal modulus and shear modulus of the tissue. It is of great significance in the mechanical characteristics of the biological tissue to be tested, and can provide a scientific basis for the early diagnosis, treatment and prevention of diseases of the biological tissue to be tested, and provide a technical path for the development of tissue biomechanical property detection equipment and lay a key technical foundation. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0057] Figure 1 This is a schematic diagram of a device for simultaneous detection of longitudinal modulus and shear modulus of biological tissue in one embodiment of the present application;

[0058] Figure 2 This is a schematic structural diagram of a device for synchronously detecting the longitudinal modulus and shear modulus of biological tissue in one embodiment of the present application;

[0059] Figure 3 Schematic diagram of a first angle between a three-dimensional translation stage, a first focusing lens, and a second focusing lens in one embodiment of the present application;

[0060] Figure 4 Schematic diagram of the second included angle among the three-dimensional translation stage, the first focusing lens, and the second focusing lens in one embodiment of the present application.

[0061] Figure 1: Laser 1; first reflector 2; half-wave plate 3; polarization beam splitter 4; quarter-wave plate 5; second reflector 6; first focusing objective lens 7; third reflector 8; second focusing objective lens 9; scanning device 10; fiber coupler 11; optical fiber 12; spectrometer 13; collimator 14; first cylindrical lens 15; first virtual imaging phase array 16; fourth reflector 17; second cylindrical lens 18; first lens 19; fifth reflector 20; second virtual imaging phase array 21; second lens 22; signal collector 23; three-dimensional translation stage 25; biological tissue to be measured 24. DETAILED DESCRIPTION

[0062] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0063] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the present application is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0064] In an exemplary embodiment, Figures 1 to 4As shown, a device for synchronously detecting the longitudinal modulus and shear modulus of biological tissue is provided, comprising: a three-dimensional translation stage 25, a dual-angle Brillouin scattering signal generating and receiving component, a fiber coupler 11 and a measuring component.

[0065] The three-dimensional displacement stage 25 is arranged on the dual-angle input and output optical paths of the dual-angle Brillouin scattering signal generating and receiving component. The fiber coupler 11 is arranged on the coupled output optical path of the dual-angle Brillouin scattering signal generating and receiving component. The measurement component is connected to the fiber coupler 11 via the optical fiber 12. The three-dimensional displacement stage 25 is used to place the biological tissue 24 to be measured. The three-dimensional displacement stage 25 is used to drive the biological tissue 24 to be measured to move so that the dual-angle focusing point of the dual-angle Brillouin scattering signal generating and receiving component coincides with the target detection point. The dual-angle Brillouin scattering signal generating and receiving component is used to emit laser light from two angles and focus it on the target detection point on the biological tissue 24 to be measured. The dual-angle Brillouin scattering signal generating and receiving component is also used to receive the Brillouin scattering signal at the target detection point. The Brillouin scattering signal includes backscattered light, first sidescattered light, and second sidescattered light. The Brillouin scattering signal is generated after the biological tissue 24 to be measured at the target detection point is irradiated by the laser light at two angles. The fiber coupler 11 is used to couple the backscattered light, first sidescattered light, and second sidescattered light at the target detection point into a coupled beam, which is then fed into the measurement assembly. The measurement assembly is used to separate the coupled beam into Brillouin scattering signals and collect the Brillouin scattering signals. The Brillouin scattering signals are used to represent the longitudinal modulus and shear modulus at the target detection point.

[0066] The dual-angle Brillouin scattering signal generating and receiving assembly includes: a laser emitting assembly, a polarization beam splitter 4, a first angle input and output optical path assembly, and a second angle input and output optical path assembly. The polarization beam splitter 4 is disposed on the output optical path of the laser emitting assembly. The first angle input and output optical path assembly is disposed on the first angle input and output optical path of the polarization beam splitter 4. The second angle input and output optical path assembly is disposed on the second angle input and output optical path of the polarization beam splitter 4. The laser emitting assembly is configured to emit laser light. The polarization beam splitter 4 is configured to split the laser light into a reflected beam and a transmitted beam. The first angle input and output optical path assembly is configured to focus the reflected beam onto a target detection point. The first angle input and output optical path is also configured to obtain backscattered light and first sidescattered light. The second angle input and output optical path assembly is configured to focus the transmitted beam onto the target detection point. The second angle input and output optical path is also configured to obtain second sidescattered light. The reflected output optical path and the transmitted output optical path of the polarization beam splitter 4 are collinear. The reflected output optical path and the transmitted output optical path of the polarization beam splitter 4 serve as coupled output optical paths of the dual-angle Brillouin scattering signal generating and receiving assembly. The reflected output light path of the polarization beam splitter 4 is the reflected output light path of the second side scattered light through the polarization beam splitter 4. The transmitted output light path of the polarization beam splitter 4 is the transmitted output light path of the backscattered light and the first side scattered light through the polarization beam splitter 4.

[0067] The laser emission assembly includes: a laser 1, a first reflector 2, and a half-wave plate 3. The first reflector 2 is disposed in the output optical path of the laser 1. The half-wave plate 3 is disposed in the output optical path of the first reflector 2. A polarization beam splitter 4 is disposed in the output optical path of the half-wave plate 3. The laser 1 is a 532nm laser or a 780nm laser.

[0068] The first angle input and output optical path assembly includes a quarter-wave plate 5, a second reflector 6, and a first focusing lens 7. The quarter-wave plate 5 is disposed in the first angle input and output optical path of the polarization beam splitter 4. The second reflector 6 is disposed in the output optical path of the quarter-wave plate 5. The first focusing lens 7 is disposed in the output optical path of the second reflector 6. The three-dimensional translation stage 25 is disposed in the output optical path of the first focusing lens 7.

[0069] The second angle input and output optical path assembly includes a third reflector 8 and a second focusing lens 9. The third reflector 8 is disposed on the second angle input and output optical path of the polarization beam splitter 4. The second focusing lens 9 is disposed on the output optical path of the third reflector 8. The three-dimensional translation stage 25 is disposed on the output optical path of the second focusing lens 9.

[0070] The measurement assembly includes a spectrometer 13 and a signal collector 23. The spectrometer 13 is connected to the fiber coupler 11 via an optical fiber 12. The spectrometer 13 is used to separate the coupled light beam into Brillouin scattering signals. The signal collector 23 is located in the output optical path of the spectrometer 13 and is used to collect the Brillouin scattering signals. The signal collector 23 is an electron multiplying CCD.

[0071] The spectrometer 13 includes a collimator 14, a first cylindrical lens 15, a first virtual imaging phase array 16, a fourth reflector 17, a second cylindrical lens 18, a first lens 19, a fifth reflector 20, a second virtual imaging phase array 21, and a second lens 22. The collimator 14 is connected to the fiber coupler 11 via an optical fiber 12. The first cylindrical lens 15 is disposed on the output optical path of the collimator 14. The first virtual imaging phase array 16 is disposed on the output optical path of the first cylindrical lens 15. The fourth reflector 17 is disposed on the output optical path of the first virtual imaging phase array 16. The second cylindrical lens 18 is disposed on the output optical path of the fourth reflector 17. The first lens 19 is disposed on the output optical path of the second cylindrical lens 18. The fifth reflector 20 is disposed on the output optical path of the first lens 19. The second virtual imaging phase array 21 is disposed on the output optical path of the fifth reflector 20. The second lens 22 is disposed on the output optical path of the second virtual imaging phase array 21. The signal collector 23 is disposed on the output optical path of the second lens 22.

[0072] A first bracket and a second bracket are provided on the 3D translation stage 25. The first bracket is rotatably connected to the 3D translation stage 25. The first bracket is used to hold the first focusing lens 7. The second bracket is rotatably connected to the 3D translation stage 25. The second bracket is used to hold the second focusing lens 9. Rotating the first and second brackets can adjust the angle of the Brillouin scattering signal.

[0073] As another embodiment, Figures 1 to 4 , a synchronous detection device for the longitudinal modulus and shear modulus of biological tissue, including: a dual-angle Brillouin scattering signal generating device, a dual-angle signal receiving device, a spectrometer 13 that can simultaneously distinguish two types of angular dispersion, a scanning device 10 and a signal collector 23; the scanning device 10 can realize multi-dimensional scanning; the dual-angle Brillouin scattering signal generating device and the dual-angle signal receiving device share some units; the dual-angle signal receiving device can be used to simultaneously receive Brillouin scattering signals of two different angles of the biological tissue to be measured 24 and collect them into the same signal collector, and perform three-dimensional synchronous measurement of the longitudinal modulus and shear modulus of the biological tissue to be measured 24 through a three-dimensional displacement stage 25.

[0074] The dual-angle Brillouin signal generating device includes: a laser 1, a first reflector 2, a half-wave plate 3, a polarization beam splitter 4, a quarter-wave plate 5, a second reflector 6, a first focusing objective lens 7, a third reflector 8 and a second focusing objective lens 9, a scanning device 10, a fiber coupler 11 and an optical fiber 12. The dual-angle Brillouin signal generating device is used to collect all Brillouin scattering spectrum signals and transmit them to the spectrometer 13; the scanning device 10 includes: a biological tissue to be tested 24 and a three-dimensional displacement stage 25. The scanning device 10 is used to move the biological tissue to be tested so that the Brillouin scattering frequency shift of the tissue can be measured in the three-dimensional direction.

[0075] The Brillouin signal is emitted by laser 1 and directed toward first reflector 2, which reflects the laser light onto half-wave plate 3. The laser light is then split by polarization beam splitter 4 into quarter-wave plate 5 and third reflector 8. Light passing through quarter-wave plate 5 is reflected by second reflector 6 into first focusing objective lens 7, where it illuminates biological tissue 24 to be measured, generating Brillouin scattering. Light passing through third reflector 8 is reflected into second focusing objective lens 9 and illuminates biological tissue 24 to be measured, generating Brillouin scattering. Laser 1 is a single-mode, narrow-linewidth laser with a wavelength of 532nm or 780nm; however, the wavelength of laser 1 is not limited to 532nm and 780nm. Polarization beam splitter 4 is a PBS polarization beam splitter.

[0076] The first focusing lens 7 focuses the laser onto the biological tissue 24 to be tested and generates a Brillouin scattering signal through photoacoustic action. Backward Brillouin scattered light returns to the first focusing lens 7 along the original path, is reflected by the second reflector 6 onto the quarter-wave plate 5, enters the polarization beam splitter 4 through the quarter-wave plate 5, and is transmitted to the fiber coupler 11. The first focusing lens 7 focuses the laser onto the biological tissue 25 to be tested and generates a Brillouin scattering signal through photoacoustic action. Side Brillouin scattered light enters the second focusing lens 9 along the direction of the second focusing lens 9, is reflected by the third reflector 8 into the polarization beam splitter 4, and part of the side scattered light is then reflected by the polarization beam splitter 4 into the fiber coupler 11. Furthermore, the second focusing lens 9 focuses the laser onto the biological tissue 24 to be tested and generates a Brillouin scattering signal through photoacoustic action. Side Brillouin scattered light enters the first focusing lens 7 along the direction of the first focusing lens 7, is reflected by the second reflector 6 into the quarter-wave plate 5, and part of the side scattered light that passes through the quarter-wave plate 5 is transmitted by the polarization beam splitter 4 into the fiber coupler 11.

[0077] After the laser 1 outputs the laser, it passes through the half-wave plate 3 to change the polarization state of the laser. When the laser passes through the polarization beam splitter 4, part of the light is transmitted and part of the light is reflected. When the first focusing lens 7 receives the backward 180° scattered light, the other second focusing lens 9 will also receive the non-180° Brillouin scattered light. Figure 3 and Figure 4 By changing the angle between the first focusing lens 7 and the second focusing lens 9, Brillouin scattered light at multiple angles including 180° and 90° can be obtained.

[0078] The dual-angle signal receiving device includes: a first focusing lens 7, a second focusing lens 9, a quarter-wave plate 5, a second reflector 6, a third reflector 8, a polarization beam splitter 4, a fiber coupler 11, and an optical fiber 12. The dual-angle signal receiving device is used to collect dual-angle Brillouin scattering spectrum signals and transmit the dual-angle Brillouin scattering spectrum signals to a spectrometer 13. The signal collector 23 includes an electron multiplying CCD and is used to collect Brillouin signals to generate sample shear modulus information. The electron multiplying CCD can be replaced with other photoelectric receivers. During each measurement, the lasers emitted by the first focusing lens 7 and the second focusing lens 9 are simultaneously focused on the same point on the biological tissue to be measured 24 for signal acquisition, and then scanned and measured point by point.

[0079] The technical features of the above embodiments can be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0080] This document uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only intended to help understand the device and its core concept of this application. At the same time, those skilled in the art will find that the specific implementation methods and application scopes may vary based on the concept of this application. In summary, the contents of this specification should not be construed as limiting this application.

Claims

1. A device for synchronously detecting longitudinal modulus and shear modulus of biological tissue, characterized in that: include: Three-dimensional translation stage, dual-angle Brillouin scattering signal generating and receiving components, fiber optic coupler and measurement components; The three-dimensional translation stage is arranged on the dual-angle input and output optical paths of the dual-angle Brillouin scattering signal generating and receiving component; the optical fiber coupler is arranged on the coupled output optical path of the dual-angle Brillouin scattering signal generating and receiving component; the measurement component is connected to the optical fiber coupler via an optical fiber; The three-dimensional translation stage is used to place the biological tissue to be tested; the three-dimensional translation stage is used to drive the biological tissue to be tested to move so that the dual-angle focusing point of the dual-angle Brillouin scattering signal generating and receiving component coincides with the target detection point; The dual-angle Brillouin scattering signal generating and receiving component is used to emit laser light from two angles and focus on a target detection point on the biological tissue to be tested; the dual-angle Brillouin scattering signal generating and receiving component is also used to receive a Brillouin scattering signal at the target detection point; the Brillouin scattering signal includes backscattered light, first side scattered light, and second side scattered light; the Brillouin scattering signal is generated by the biological tissue to be tested at the target detection point after being irradiated by the laser light at two angles; The optical fiber coupler is used to couple the backscattered light, the first sidescattered light and the second sidescattered light at the target detection point into a coupled light beam, and input the coupled light beam into the measurement component; The measurement component is used to separate the coupled light beam into Brillouin scattering signals and collect the Brillouin scattering signals; the Brillouin scattering signals are used to express the longitudinal modulus and shear modulus at the target detection point.

2. The device for synchronously detecting the longitudinal modulus and shear modulus of biological tissue according to claim 1, wherein: The dual-angle Brillouin scattering signal generating and receiving component includes: a laser emitting component, a polarization beam splitter, a first angle input and output optical path component and a second angle input and output optical path component; The polarization beam splitter is arranged on the output light path of the laser emitting assembly; The first angle input and output optical path component is arranged on the first angle input and output optical path of the polarization beam splitter; The second angle input and output optical path component is arranged on the second angle input and output optical path of the polarization beam splitter; The laser emitting component is used to emit laser; The polarization beam splitter is used to split the laser into a reflected light beam and a transmitted light beam; The first angle input and output optical path component is used to focus the reflected light beam to the target detection point; the first angle input and output optical path is also used to obtain backscattered light and first side scattered light; The second angle input and output optical path component is used to focus the transmitted light beam to the target detection point; the second angle input and output optical path is also used to obtain the second side scattered light; The reflected output light path and the transmitted output light path of the polarization beam splitter are collinear; the reflected output light path and the transmitted output light path of the polarization beam splitter serve as coupled output light paths of the dual-angle Brillouin scattering signal generating and receiving component; The reflected output light path of the polarization beam splitter is the reflected output light path of the second side scattered light through the polarization beam splitter; the transmitted output light path of the polarization beam splitter is the transmitted output light path of the backscattered light and the first side scattered light through the polarization beam splitter.

3. The device for synchronously detecting the longitudinal modulus and shear modulus of biological tissue according to claim 2, wherein: The laser emission assembly includes: a laser, a first reflector and a half-wave plate; The first reflector is arranged on the output optical path of the laser; The half-wave plate is arranged on the output optical path of the first reflector; The polarization beam splitter is arranged on the output light path of the half wave plate.

4. The device for synchronously detecting the longitudinal modulus and shear modulus of biological tissue according to claim 3, characterized in that: The laser is a 532nm laser or a 780nm laser.

5. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 3, characterized in that: The first angle input and output optical path components include: a quarter wave plate, a second reflector and a first focusing objective lens; The quarter wave plate is arranged on the first angle input and output optical path of the polarization beam splitter; The second reflector is arranged on the output optical path of the quarter-wave plate; The first focusing objective lens is arranged on the output light path of the second reflecting mirror; The three-dimensional translation stage is arranged on the output optical path of the first focusing objective lens.

6. The device for simultaneous detection of longitudinal modulus and shear modulus of biological tissue according to claim 5, characterized in that: The second angle input and output optical path component includes: a third reflector and a second focusing objective lens; The third reflector is arranged on the second angle input and output optical path of the polarization beam splitter; The second focusing objective lens is arranged on the output light path of the third reflecting mirror; The three-dimensional translation stage is arranged on the output optical path of the second focusing objective lens.

7. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 3, characterized in that: The measuring component includes: a spectrometer and a signal collector; The spectrometer is connected to the fiber coupler via an optical fiber; the spectrometer is used to separate the coupled light beam into Brillouin scattering signals; The signal collector is arranged on the output optical path of the spectrometer; the signal collector is used to collect Brillouin scattering signals.

8. The device for simultaneous detection of longitudinal modulus and shear modulus of biological tissue according to claim 7, characterized in that: The spectrometer comprises: a collimator, a first cylindrical lens, a first virtual imaging phase array, a fourth reflector, a second cylindrical lens, a first lens, a fifth reflector, a second virtual imaging phase array and a second lens; The collimator is connected to the fiber coupler via an optical fiber; The first cylindrical lens is arranged on the output light path of the collimator; The first virtual imaging phase array is arranged on the output optical path of the first cylindrical lens; The fourth reflector is arranged on the output optical path of the first virtual imaging phase array; The second cylindrical lens is arranged on the output light path of the fourth reflector; The first lens is arranged on the output light path of the second cylindrical lens; The fifth reflector is arranged on the output light path of the first lens; The second virtual imaging phase array is arranged on the output optical path of the fifth reflector; The second lens is arranged on the output optical path of the second virtual imaging phase array; The signal collector is arranged on the output optical path of the second lens.

9. The device for simultaneous detection of longitudinal modulus and shear modulus of biological tissue according to claim 7, characterized in that: The signal collector is an electron multiplying CCD.

10. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 6, characterized in that: The three-dimensional displacement platform is provided with a first bracket and a second bracket; The first bracket is rotatably connected to the three-dimensional translation stage; the first bracket is used to place the first focusing objective lens; The second bracket is rotatably connected to the three-dimensional translation stage; the second bracket is used to place the second focusing objective lens; Rotating the first bracket and the second bracket can adjust the angle of the Brillouin scattering signal.

Images