Automatic navigation sea water component analysis system

Abstract

The invention discloses an automatic navigation sea water component analysis system, and relates to the technical field of sea water component analysis, and the system comprises an upper computer which is used for controlling the initialization, preheating, cyclic detection and data storage processes of the whole system; the peristaltic pump is used for transmitting the seawater sample in the underway process into the quartz flow cell according to preset flow and time parameters; the fluorospectro photometer sample cell is made of a quartz flow cell, one end of the fluorospectro photometer sample cell is used for receiving the seawater sample transmitted by the peristaltic pump, and the other end of the fluorospectro photometer sample cell is used for discharging detected liquid; the waste liquid collecting pool is used for receiving waste liquid discharged after detection of the fluorospectro photometer, so that the purpose of replacing a sample without opening a cover to move the sample pool is achieved, the experiment efficiency is improved, human errors are reduced, the instrument can continuously extract navigation water for detection and analysis, human errors and operation errors are reduced, the efficiency is greatly improved, and the device is suitable for popularization and application. And labor and time costs are reduced.

Description

Technical Field

[0001] The invention relates to the technical field of seawater component analysis, and in particular to an automated navigation seawater component analysis system. Background Art

[0002] Fluorescence spectrophotometer is a precision instrument used to measure the fluorescence intensity of substances. By measuring the fluorescence excitation and emission spectra of fluorescent substances in samples, different substances can be qualitatively identified, because different substances have different fluorescence characteristic spectra. In marine scientific observations, fluorescence spectrophotometers can be used to detect a variety of important components, including chlorophyll, nutrients, dissolved organic matter, etc. The determination of these components is crucial to understanding the productivity of marine ecosystems, organic carbon cycles, and the distribution and impact of pollutants. Through the use of fluorescence spectrophotometers, researchers can accurately and quickly obtain these key data, providing strong support for marine scientific research. With the continuous advancement of technology, instrument automation has been widely used in scientific research, industry and many other fields. Applying the concept of automation to the analysis and detection of seawater components is likely to make scientific research more convenient and efficient.

[0003] At present, in marine scientific observation and research, if you want to use a fluorescence spectrophotometer to detect some important components in seawater, the experimenters need to first arrive at the designated location with the ship to collect water. In this process, continuous sampling and water collection are usually required to ensure the continuity and accuracy of the data, which is time-consuming and labor-intensive, and places high demands on the experimenters; after collecting water, these seawater samples need to be stored in pre-processed storage bottles until they return to the laboratory after getting off the ship and then tested with a fluorescence spectrophotometer. In this process, the experimenters need to test a large number of samples stored after continuous sampling, which consumes a lot of energy; at the same time, during the journey, the storage bottles themselves are at risk of being fragile, which may pose a potential threat to safety and sample integrity. Summary of the invention

[0004] The purpose of the present invention is to change the sample pool in the fluorescence spectrophotometer from the traditional quartz cuvette to a quartz flow cell, connect the two glass tubes at the top of the flow cell to the silicone tube, and inject the sample at one end and discharge the sample at the other end, so that the detection sample can be replaced without opening the cover; the peristaltic pump is connected to the silicone tube at the injection end of the flow cell, and the seawater is transmitted to the flow cell according to a certain flow rate for a specified time; the upper computer software controls the peristaltic pump to transmit the sample, and controls the fluorescence spectrophotometer to detect the sample. This process is realized to improve the accuracy and continuity of the measurement, reduce human errors and operational errors. Secondly, the efficiency can be greatly improved, and the labor and time costs can be reduced. In addition, the experimental parameters can be monitored in real time and the equipment status can be automatically adjusted to ensure the stability and reliability of the experimental process. Finally, it is also helpful to improve the credibility of data quality and analysis results, and provide more accurate and reliable data support for scientific researchers.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] An automated seawater composition analysis system, comprising:

[0007] The host computer is used to control the initialization, preheating, cycle detection and data storage process of the entire system.

[0008] A peristaltic pump is used to transfer seawater samples during the navigation process to a quartz circulation pool according to preset flow and time parameters.

[0009] A fluorescence spectrophotometer, wherein the sample cell of the fluorescence spectrophotometer is made of a quartz flow cell, one end of which is used to receive the seawater sample transmitted by a peristaltic pump, and the other end of which is used to discharge the liquid after detection.

[0010] The waste liquid collection tank is used to receive the waste liquid discharged after detection by the fluorescence spectrophotometer.

[0011] The above technical solution further includes:

[0012] The host computer is connected with the peristaltic pump and the fluorescence spectrophotometer through a serial port, and the host computer is used to control the operation of the peristaltic pump and the detection of the fluorescence spectrophotometer.

[0013] One end of the quartz flow cell in the fluorescence spectrophotometer is fixedly connected with a first silicone tube, the first silicone tube is fixedly connected to a peristaltic pump, and the first silicone tube is used to receive the seawater sample transmitted by the peristaltic pump.

[0014] The other end of the quartz flow cell in the fluorescence spectrophotometer is fixedly connected with a second silicone tube, the second silicone tube is fixedly connected to the waste liquid collection tank, and the second silicone tube is used to discharge the detected liquid into the waste liquid collection tank.

[0015] The host computer includes a control module. After the host computer is started, the control module first initializes the system to turn on the power of the fluorescence spectrophotometer and the peristaltic pump, waits for the fluorescence spectrophotometer to preheat for 15-20 minutes, and ensures that the instrument reaches a stable state. The control module sends parameter settings through the serial port to determine the flow rate and rotation direction of the peristaltic pump and controls the peristaltic pump to transmit the navigation seawater to the circulation pool. After the detection is completed, the control module can control the shutdown of the host computer, the peristaltic pump, the fluorescence spectrophotometer and other components.

[0016] The host computer includes a spectrum correction module, which establishes a spectrum correction model, regularly performs spectrum correction on the fluorescence spectrophotometer, collects a series of standard solutions of known concentrations, measures their fluorescence spectra, and establishes a spectrum correction model using a polynomial fitting method. In actual measurement, the spectrum correction model is used to correct the measurement results, wherein the formula of the spectrum correction model is expressed as: Y 校正 =α0+α1X+α2X 2 +...+α n X n , where Y 校正 is the corrected data, X is the original data, α i is the fitting coefficient.

[0017] The fluorescence spectrophotometer includes a detection module, which is responsible for controlling the fluorescence spectrophotometer to accurately measure the fluorescence intensity of the sample. The detection parameter setting instructions, including excitation wavelength, emission wavelength, scanning range, integration time, etc., are sent to the detection module through the serial port. After receiving the instructions, the detection module performs parameter configuration and returns a confirmation signal to the host computer. The detection module performs spectral scanning according to the preset parameters and records the fluorescence excitation and emission spectra of the sample. After the detection is completed, the detection module transmits the collected fluorescence spectrum data to the host computer through the serial port.

[0018] The host computer includes a storage module, which saves the processed data to a local storage unit to ensure the security and traceability of the data. The storage unit can upload the data to a cloud server or a laboratory database for remote backup and sharing.

[0019] The host computer includes an alarm module, which checks whether the peristaltic pump successfully receives the set parameters. If the peristaltic pump does not successfully receive the parameters, the host computer continues to send the parameters and continues to send instructions until it succeeds or an alarm is triggered after exceeding the set number of times. It checks whether the peristaltic pump operates normally. If it does not operate normally, the host computer continues to send operation instructions until it succeeds or an alarm is triggered after exceeding the set number of times. It checks whether the fluorescence spectrophotometer successfully receives and executes the instructions. If an abnormality is detected, the host computer continues to send instructions until it succeeds or an alarm is triggered after exceeding the set number of times. It checks whether the data is successfully saved. If not, the host computer continues to try to save until it succeeds or an alarm is triggered.

[0020] Among them, the alarm limit for the peristaltic pump receiving instructions and whether it operates normally is set to 5 times. If it exceeds 5 times, the host computer will alarm the mobile terminal. The alarm limit for whether the fluorescence spectrophotometer successfully receives and executes instructions is also set to alarm if it exceeds 5 times.

[0021] The present invention has the following beneficial effects:

[0022] 1. In the present invention, the sample cell in the fluorescence spectrophotometer is replaced by a quartz flow cell from the traditional quartz cuvette, and the flow cell is connected to a hose, so that the purpose of replacing the sample can be achieved without opening the cover and moving the sample cell, thereby improving the experimental efficiency and reducing human errors. At the same time, it is an important part of realizing automatic navigation water.

[0023] 2. In the present invention, a peristaltic pump is used to transfer seawater samples and accurately control the flow rate; physical damage to the samples is avoided and the integrity of the samples is maintained; compared with the traditional method of storing the samples in bottles and then returning them to the laboratory for testing, the peristaltic pump is used for on-site transmission, which is easy to maintain and clean; in conjunction with the host computer, the purpose of automation is achieved, and multiple cycle testing can be realized without manual intervention, thereby improving the detection efficiency of the device.

[0024] 3. In the present invention, the peristaltic pump is controlled by the host computer program to realize the transmission of liquid by timing operation at a certain speed, and the fluorescence spectrophotometer realizes the timing detection of samples, and the two together form a cycle. Under the control of this program, the instrument can continuously extract the seawater for detection and analysis, reduce human errors and operational errors, greatly improve efficiency, and reduce labor and time costs.

[0025] 4. In the present invention, the host computer is used for real-time monitoring to ensure that researchers can find problems in time and adjust the equipment status in time, thereby ensuring the accuracy and continuity of the experiment. The correction module corrects the measurement results of the fluorescence spectrophotometer by establishing a spectral correction model, thereby significantly improving the accuracy of seawater component analysis. This correction can eliminate the influence of instrument errors and external interference on the measurement results, making the analysis results more reliable. Regular spectral correction can ensure that the fluorescence spectrophotometer maintains stable performance during long-term use. By collecting standard solutions of known concentrations and measuring their fluorescence spectra, the correction module can promptly discover and adjust the deviation of the instrument, thereby extending the service life of the instrument and reducing maintenance costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the structure of an automated navigational seawater composition analysis system proposed by the present invention;

[0027] Figure 2 It is a schematic diagram of the operation steps of the device in the present invention;

[0028] Figure 3 It is a flowchart of the specific instructions of the device in the present invention.

[0029] In the figure: 1. host computer; 2. peristaltic pump; 3. first silicone tube; 4. fluorescence spectrophotometer; 5. second silicone tube; 6. waste liquid collection tank. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Embodiment 1

[0032] like Figure 1-Figure 3 As shown, the present invention proposes an automated navigational seawater composition analysis system, comprising:

[0033] Host computer 1 is used to control the initialization, preheating, cycle detection and data storage process of the entire system.

[0034] Peristaltic pump 2 is used to transfer seawater samples during the navigation process to the quartz circulation pool according to preset flow and time parameters.

[0035] Fluorescence spectrophotometer 4, the sample cell of fluorescence spectrophotometer 4 is made of a quartz flow cell, one end of which is used to receive the seawater sample transmitted by the peristaltic pump, and the other end of which is used to discharge the liquid after detection.

[0036] The waste liquid collection tank 6 is used to receive the waste liquid discharged after detection by the fluorescence spectrophotometer 4 .

[0037] The host computer 1 is connected to the peristaltic pump 2 and the fluorescence spectrophotometer 4 via a serial port. The host computer 1 is used to control the operation of the peristaltic pump 2 and the detection of the fluorescence spectrophotometer 4 .

[0038] One end of the quartz flow cell in the fluorescence spectrophotometer 4 is fixedly connected with a first silicone tube 3 , and the first silicone tube 3 is fixedly connected to the peristaltic pump 2 . The first silicone tube 3 is used to receive the seawater sample transmitted by the peristaltic pump 2 .

[0039] The other end of the quartz flow cell in the fluorescence spectrophotometer 4 is fixedly connected with a second silicone tube 5 , which is fixedly connected to a waste liquid collection tank 6 . The second silicone tube 5 is used to discharge the detected liquid into the waste liquid collection tank 6 .

[0040] The host computer 1 includes a control module. After the host computer 1 is started, the control module first initializes the system to turn on the power of the fluorescence spectrophotometer 4 and the peristaltic pump 2, waits for the fluorescence spectrophotometer 4 to preheat for 15-20 minutes, and ensures that the instrument reaches a stable state. The control module sends parameter settings through the serial port to determine the flow rate and rotation direction of the peristaltic pump 2 and controls the peristaltic pump 2 to transmit the sailing seawater to the circulation pool. After the detection is completed, the control module can control the shutdown of the host computer 1, the peristaltic pump 2, the fluorescence spectrophotometer 4 and other components.

[0041] The host computer 1 includes a spectrum correction module, which establishes a spectrum correction model, regularly performs spectrum correction on the fluorescence spectrophotometer 4, collects a series of standard solutions of known concentrations, measures their fluorescence spectra, and establishes a spectrum correction model using a polynomial fitting method. In actual measurement, the spectrum correction model is used to correct the measurement results, wherein the formula of the spectrum correction model is expressed as: 校正 =α0+α1X+α2X 2 +...+α n X n , where Y 校正 is the corrected data, X is the original data, α i is the fitting coefficient.

[0042] The fluorescence spectrophotometer 4 includes a detection module, which is responsible for controlling the fluorescence spectrophotometer 4 to accurately measure the fluorescence intensity of the sample. The detection parameter setting instructions, including the excitation wavelength, emission wavelength, scanning range, integration time, etc., are sent to the detection module through the serial port. After receiving the instructions, the detection module configures the parameters and returns a confirmation signal to the host computer 1. The detection module performs spectral scanning according to the preset parameters and records the fluorescence excitation and emission spectra of the sample. After the detection is completed, the detection module transmits the collected fluorescence spectrum data to the host computer 1 through the serial port.

[0043] The host computer 1 includes a storage module, which saves the processed data to a local storage unit to ensure the security and traceability of the data. The storage unit can upload the data to a cloud server or a laboratory database for remote backup and sharing.

[0044] The host computer 1 includes an alarm module, which checks whether the peristaltic pump 2 successfully receives the set parameters. If the peristaltic pump 2 does not successfully receive the parameters, the host computer 1 continues to send the parameters and the host computer 1 continues to send instructions until it succeeds or an alarm is issued after exceeding the set number of times. It checks whether the peristaltic pump 2 operates normally. If it does not operate normally, the host computer 1 continues to send operation instructions until it succeeds or an alarm is issued after exceeding the set number of times. It checks whether the fluorescence spectrophotometer 4 successfully receives and executes the instructions. If an abnormality is detected, the host computer 1 continues to send instructions until it succeeds or an alarm is issued after exceeding the set number of times. It checks whether the data is successfully saved. If not, the host computer 1 continues to try to save until it succeeds or an alarm is issued.

[0045] In this embodiment, after the host computer 1 is started, the fluorescence spectrophotometer 4 is turned on. After the fluorescence spectrophotometer 4 is ready, the software in the host computer 1 that controls the fluorescence spectrophotometer 4 for detection is opened, and initialization is started. After the initialization is completed, the instrument is waited for 15-20 minutes to preheat. During the preheating process, the instrument will reach certain temperature, humidity, stability and other conditions, eliminate the error of the instrument itself, improve the accuracy and stability of the fluorescence intensity measurement, and ensure that the detection result is accurate and reliable. After the preheating is completed, the process starts.

[0046] First, the host computer 1 sends instructions to the peristaltic pump 2 through the control module to set the flow rate and the rotation direction of the pump. The time for the instruction setting parameters can be reserved for 30s. If the pump fails to achieve the parameter setting, the host computer will continue to send the parameter setting instruction through the alarm module; if it fails to achieve more than 5 times, the host computer will alarm the mobile terminal through the alarm module. Then, after the parameter setting is successful, the host computer 1 controls the peristaltic pump 2 to start running through the control module, and transmits the traveling seawater to the circulation pool through the first silicone tube 3. The operation time can be set to 5 minutes. A long enough time should be reserved here to ensure that there is enough liquid in the circulation pool for detection and to achieve the purpose of rinsing the circulation pool. If the peristaltic pump 2 does not operate normally, the host computer 1 will continue to send operation instructions. If it does not operate normally for more than 5 times, the host computer 1 will alarm the mobile terminal through the alarm module. Secondly, after the peristaltic pump 2 ends its operation, the host computer 1 sends a fluorescence spectrophotometer The detection module of 4 sends an instruction to start detection. If the detection is abnormal, the operation is the same as using the alarm module to alarm. After the detection is completed, the obtained data is named with the current date and time through the storage module to facilitate subsequent data analysis. The host computer 1 monitors whether the fluorescence spectrophotometer 4 has completed the detection and completed the storage and naming of the data. If it has been completed, a cycle is completed, and the waste liquid after the detection is transmitted to the waste liquid collection tank 6 through the second silicone tube for storage and processing; if it is not completed, the host computer alarms the mobile terminal through the alarm module, and then enters the next cycle. The host computer 1 controls the peristaltic pump 2 to start running again. After the operation is completed, the instrument starts to detect. After the instrument detection is completed and the data is saved, the peristaltic pump 2 is turned on again, and multiple cycles are performed. Note that when the entire experiment is over, the xenon lamp in the fluorescence spectrophotometer 4 must be turned off for 15 minutes to allow it to cool sufficiently, and then the overall instrument switch is turned off.

[0047] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An automated seawater composition analysis system, characterized in that: include: A host computer (1), the host computer (1) is used to control the initialization, preheating, cycle detection and data storage process of the entire system; A peristaltic pump (2), the peristaltic pump (2) being used to transfer the seawater sample during the navigation process to the quartz circulation pool according to preset flow and time parameters; A fluorescence spectrophotometer (4), wherein the sample pool of the fluorescence spectrophotometer (4) is made of a quartz flow cell, one end of which is used to receive the seawater sample transmitted by a peristaltic pump, and the other end of which is used to discharge the liquid after detection; A waste liquid collection pool (6) is used to receive waste liquid discharged after detection by the fluorescence spectrophotometer (4).

2. The automated seawater composition analysis system according to claim 1, characterized in that: The host computer (1) is connected to the peristaltic pump (2) and the fluorescence spectrophotometer (4) via a serial port, and the host computer (1) is used to control the operation of the peristaltic pump (2) and the detection of the fluorescence spectrophotometer (4).

3. The automated seawater composition analysis system according to claim 1, characterized in that: A first silicone tube (3) is fixedly connected to one end of the quartz flow cell in the fluorescence spectrophotometer (4), the first silicone tube (3) is fixedly connected to the peristaltic pump (2), and the first silicone tube (3) is used to receive the seawater sample transmitted by the peristaltic pump (2).

4. The automated seawater composition analysis system according to claim 1, characterized in that: The other end of the quartz flow cell in the fluorescence spectrophotometer (4) is fixedly connected to a second silicone tube (5), the second silicone tube (5) is fixedly connected to the waste liquid collection tank (6), and the second silicone tube (5) is used to discharge the detected liquid into the waste liquid collection tank (6).

5. The automated seawater composition analysis system according to claim 1, characterized in that: The host computer (1) comprises a control module. The control module enables the host computer (1) to, after starting up, first initialize the system to turn on the power of the fluorescence spectrophotometer (4) and the peristaltic pump (2), wait for the fluorescence spectrophotometer (4) to be preheated for 15-20 minutes, and ensure that the instrument reaches a stable state. The control module sends parameter settings through a serial port to determine the flow rate and rotation direction of the peristaltic pump (2) and control the peristaltic pump (2) to transmit the navigation seawater to the circulation pool. After the detection is completed, the control module controls the host computer (1), the peristaltic pump (2), the fluorescence spectrophotometer (4) and other components to be turned off.

6. The automated seawater composition analysis system according to claim 1, characterized in that: The host computer (1) comprises a spectrum correction module, which establishes a spectrum correction model, regularly performs spectrum correction on the fluorescence spectrophotometer (4), collects a series of standard solutions with known concentrations, measures their fluorescence spectra, and establishes a spectrum correction model using a polynomial fitting method. In actual measurement, the spectrum correction model is used to correct the measurement results, wherein the spectrum correction model is expressed as follows: 校正 =α0+α1X+α2X 2 +...+α n X n , where Y 校正 is the corrected data, X is the original data, α i is the fitting coefficient.

7. The automated seawater composition analysis system according to claim 1, characterized in that: The fluorescence spectrophotometer (4) comprises a detection module, which is responsible for controlling the fluorescence spectrophotometer (4) to measure the fluorescence intensity of the sample. A detection parameter setting instruction including an excitation wavelength, an emission wavelength, a scanning range and an integration time is sent to the detection module via a serial port. After receiving the instruction, the detection module performs parameter configuration and returns a confirmation signal to the upper computer (1). The detection module performs spectrum scanning according to preset parameters and records the fluorescence excitation and emission spectra of the sample. After the detection is completed, the detection module transmits the collected fluorescence spectrum data to the upper computer (1) via the serial port.

8. The automated seawater composition analysis system according to claim 1, characterized in that: The host computer (1) comprises a storage module, which stores the processed data in a local storage unit, and the storage unit uploads the data to a cloud server or a laboratory database for remote backup and sharing.

9. The automated seawater composition analysis system according to claim 1, characterized in that: The host computer (1) includes an alarm module, which checks whether the peristaltic pump (2) successfully receives the set parameters. If the peristaltic pump (2) does not successfully receive the parameters, the host computer (1) continues to send the parameters until it succeeds or exceeds a set number of times and then issues an alarm. It checks whether the peristaltic pump (2) operates normally. If it does not operate normally, the host computer (1) continues to send operation instructions until it succeeds or exceeds a set number of times and then issues an alarm. It checks whether the fluorescence spectrophotometer (4) successfully receives and executes the instructions. If an abnormality is detected, the host computer (1) continues to send instructions until it succeeds or exceeds a set number of times and then issues an alarm. It checks whether the data is successfully saved. If it fails, the host computer (1) continues to try to save until it succeeds or issues an alarm.

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