A coral regenerative gel in the form of a barnacle and a method of preparing the same

Abstract

This application relates to a barnacle-like coral regeneration gel and its preparation method, belonging to the fields of marine biotechnology and bioadhesive technology. This material is designed for the restoration of coral reef ecosystems to promote coral regeneration. The gel prepared by this method not only ensures the safe and stable reproduction of coral larvae and zooxanthellae in the marine environment but also provides the necessary nutrients for coral polyp growth.

Description

Technical Field

[0001] This invention belongs to the fields of marine biotechnology and bioadhesion technology, specifically relating to a barnacle-like coral regeneration gel and its preparation method. Background Technology

[0002] Due to environmental pollution, ocean warming, and human activities, the global coral reef coverage is declining at an alarming rate. Faced with this dire situation, global attention and emphasis on restoring coral reef ecosystems are increasing. Internationally, scientists are working to protect coral biodiversity by storing coral larvae in cryopreservation facilities. In my country, coral reef restoration technology has made some progress. On October 1, 2022, my country officially implemented the "Technical Guidelines for Marine Ecological Restoration Part 2: Coral Reef Ecological Restoration" (GB / T 41339.2-2022), my country's first technical standard specifically for marine ecosystem restoration. Specific technical methods include: constructing artificial reefs, transplanting healthy corals into damaged coral reef areas, and using reinforced concrete to construct stable coral reef structures. These physical restoration methods allow coral reefs to largely recover their normal growth rate and health status after four years, but the economic cost is too high.

[0003] Currently, no effective biological methods are applied to the restoration of coral reef ecosystems. However, if biological methods are used, such as immobilization techniques to fix artificially enhanced zooxanthellae and coral larvae in gels, allowing the prepared gels to adhere to the coral skeleton like barnacles, a safe and stable environment can be provided for the development of both corals and zooxanthellae. This would improve the symbiotic interaction between coral larvae and their symbiotic zooxanthellae, enhance the coral's resistance to heat bleaching, accelerate the remodeling of calcareous skeletons, and effectively promote coral regeneration. This innovative method could offer new hope for the protection and restoration of coral reefs.

[0004] This invention prepares a barnacle-like gel material with strong biocompatibility with coral larvae and zooxanthellae. This material uses coral skeleton as a substrate for the survival of coral larvae and zooxanthellae, enabling coral larvae to reproduce safely and stably in the ocean with the help of zooxanthellae, promoting the formation of their calcareous skeleton, rapidly and efficiently reshaping the coral structure, and thus promoting the coral regeneration process. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a novel barnacle-like coral regeneration gel and its preparation method. This material is designed to restore coral reef ecosystems and promote coral regeneration. The gel prepared using this method adheres to coral skeletons like barnacles without causing environmental pollution. It not only ensures the safe and stable reproduction of coral larvae and zooxanthellae in the marine environment but also provides the necessary nutrients for coral polyp growth.

[0006] This gel carrier offers several significant advantages: First, the chosen substrate provides coral polyps with some of the calcium ions necessary for development, promoting coral larvae growth, and also serves as an effective support structure, providing a safe and stable growth space. Second, the barnacle-like gel can improve the attachment and survival rates of coral larvae and strengthen the symbiotic relationship with zooxanthellae, providing a solid foundation for the natural restoration of coral reefs. Furthermore, the environmental friendliness and biodegradability of this gel material reduce secondary pollution to the marine environment, ensuring the sustainability of restoration efforts. Compared to traditional physical remediation methods, the bioremediation approach of this invention offers lower economic costs and higher ecological benefits, providing an economical and efficient solution for the long-term protection and restoration of coral reefs.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0008] A barnacle-like coral regeneration gel, characterized by being synthesized from gel beads that initially immobilize coral larvae and zooxanthellae, coral skeleton, a bio-adhesive, and ethylene glycol diglycidyl ether (EGDE). Wherein:

[0009] The gel beads used for the initial fixation of coral larvae and zooxanthellae have the following components and their weight ratios as follows:

[0010] Zooxanthophyte Cladocopium goreaui 1-5 parts,

[0011] Coral larvae Acropora robusta 1-5 parts,

[0012] 1-3 parts of a 1-2% propylene glycol alginate solution.

[0013] Calcium chloride solution.

[0014] Bio-adhesives contain:

[0015] 1–5% 3,4-dihydroxyphenylalanine solution and 1–5% tyrosinase solution.

[0016] A barnacle-like coral regeneration gel has the following components and their weight ratios as follows:

[0017]

[0018] The preparation method of the barnacle-like coral regeneration gel includes the following steps: 1) Take the culture medium of coral larvae and zooxanthellae, mix it with propylene glycol alginate (PGA) solution, and add the mixed solution dropwise to calcium chloride solution to initially cross-link and form gel beads; 2) Place the gel beads soaked in 3,4-dihydroxyphenylalanine (DOPA) solution on the surface of coral skeleton coated with DOPA, ensuring close contact between the gel beads and the coral skeleton surface, so that DOPA forms a thin adhesive layer between the two surfaces; 3) Put the coral skeleton with the gel beads attached into tyrosinase solution to solidify DOPA; 4) Then put it into ethylene glycol diglycidyl ether (EGDE) solution to obtain the barnacle-like coral regeneration gel.

[0019] Furthermore, the preparation method of the barnacle-like coral regeneration gel includes the following steps:

[0020] 1. Preparation of gel beads for preliminary fixation of coral larvae and zooxanthellae

[0021] (1) Prepare a culture medium containing robust staghorn coral (Acropora robusta) larvae and zooxanthellae (Cladocopium goreaui).

[0022] Add 2–10 parts of filtered natural seawater and 0.5–1.5 parts of glucose to L1 medium, stir for 2 min, then slowly add 1–5 parts of robust staghorn coral (Acropora robusta) and zooxanthellae (Cladocopium goreaui) at 16.72–28.40℃. Simulate natural conditions using a 12-hour light-12-hour dark photocycle, with a light intensity of 90 μE / m. 2 Under conditions of / s, maintain ventilation in the experimental environment and incubate for 1–2 days.

[0023] (2) Preparation of propylene glycol alginate (PGA) solution:

[0024] A. Esterification reaction

[0025] Add 0.5 to 1 part of sodium alginate (SA) to the reaction flask, mix with filtered natural seawater, place on a 40°C constant temperature water bath shaker and heat until dissolved to form a 0.5% to 1.0% SA solution, then cool to room temperature.

[0026] Add 3-6 parts of filtered natural seawater, 0.5-0.9 parts of SA solution, 1.5-2.7 parts of propylene oxide, and finally 0.1-0.3 parts of sodium hydroxide to a pressure reactor. React for 3-5 hours at a working pressure of 10-20 bar and a temperature of 55-80°C, and then cool to room temperature.

[0027] B. Neutralization, extraction, and purification

[0028] After the esterification reaction, the product needs to be neutralized to prevent over-reaction or side reactions. Add an appropriate amount of acetic acid dropwise to the reaction vessel and use a pH meter to monitor the solution pH, adjusting it to approximately 6.5–7.5.

[0029] Add 3–10 parts of 95% ethanol solution to the reactor and mix at 120 rpm for 20 minutes on a water bath shaker to precipitate PGA. Filter the product, dissolve the precipitate in a small amount of water, and cool at -10°C for 30 minutes to allow PGA crystallization. Filter a second time to further remove impurities.

[0030] C. Washing and drying:

[0031] Take a reaction flask, add an appropriate amount of water, add the PGA precipitate obtained in step B, add 4-10 parts of methanol, stir for 5 minutes, and filter to remove unreacted raw materials and byproducts. Repeat this process 3-5 times.

[0032] The washed PGA was dried in a vacuum drying oven for 2 hours to remove residual solvent and moisture. After drying, the PGA formed a powdery solid.

[0033] D. Preparation of PGA solution

[0034] PGA is dissolved in water to form a PGA solution with a concentration of 1-2%.

[0035] (3) Preliminary cross-linking:

[0036] Take one reaction flask, add 1.5–4 parts calcium chloride and an appropriate amount of filtered natural seawater, and stir to form a 2–5% calcium chloride solution. In another reaction flask, take 1–3 parts of culture medium containing robust staghorn coral larvae (Acropora robusta) and zooxanthellae (Cladocopium goreaui), and mix with 1–3 parts of a 1–2% PGA solution. Using a pipette, add the mixture dropwise to the calcium chloride solution, allowing initial cross-linking to form gel beads. Let it stand for 30–60 minutes.

[0037] (4) Cleaning the gel beads:

[0038] Remove the gel beads and gently rinse them with deionized water to remove excess calcium chloride from the surface.

[0039] 2. Preparation and adhesion of bio-adhesives

[0040] (1) Preparation of 3,4-dihydroxyphenylalanine (DOPA) solution

[0041] A. Synthesis of 3-bromo-L-tyrosine

[0042] Take a reaction flask, add 5-9 parts of hydrogen bromide (HBr) and an appropriate amount of water, stir until HBr dissolves, and prepare a 48% hydrogen bromide aqueous solution. Add 2-5 parts of L-tyrosine to the hydrogen bromide aqueous solution, and place it in a constant temperature water bath shaker at 0-5℃ and mix at 120 rpm for 2 hours.

[0043] The reaction flask was then removed, the solution was heated to room temperature, and placed in a 30°C constant temperature water bath shaker to mix at 120 rpm for 30 minutes.

[0044] Take 300 ml of cold water at 10°C, quickly mix the reaction solution with the cold water, and a solid will precipitate. Filter, and then wash the solid with cold water to obtain 3-bromo-L-tyrosine.

[0045] B. Hydroxylation reaction

[0046] Take 1–3 parts of the 3-bromo-L-tyrosine obtained in step A, dissolve it in 100 ml of filtered natural seawater, add 0.2–0.5 parts of sodium hydroxide and 0.05–0.1 parts of copper iodide (CuI), and place in a 25°C constant temperature water bath shaker to mix at 120 rpm for 3 hours. Filter, add acetic acid to neutralize the filtrate to pH 6.5–7.5.

[0047] Add 95% ethanol solution to the reaction solution to precipitate DOPA. Filter and dry in a vacuum drying oven for 2 hours to obtain purified DOPA.

[0048] Dissolve purified DOPA in an appropriate amount of water to prepare a DOPA solution with a concentration of 1-5%.

[0049] (2) Soaking and coating:

[0050] At room temperature, take a DOPA solution with a concentration between 1% and 5%, and immerse 2 to 6 portions of cleaned gel beads in the solution. After 5 minutes, remove the beads and simultaneously use a sterilized wool brush to apply the DOPA solution to the surface of the coral skeleton, which should be 5 to 10 cm in size. Ensure that the gel beads and the coral skeleton surface are evenly covered with the DOPA solution.

[0051] (3) Adhesion:

[0052] At room temperature, gently place the soaked gel beads on the surface of the coral skeleton coated with DOPA solution, press the gel beads gently, and maintain pressure for about 1 to 2 minutes to ensure close contact between the gel beads and the coral skeleton surface, allowing DOPA to form a thin adhesive layer between the two surfaces.

[0053] (4) Curing DOPA:

[0054] Take a reaction flask and add 0.3–0.8 parts of tyrosinase and filtered natural seawater to form a 1–5% tyrosinase solution. Place the coral skeleton with the gel beads already attached into the solution, which will oxidize DOPA and accelerate the curing process of the DOPA adhesive. Remove the coral after standing for 1–3 hours.

[0055] 3. Synthesis of immobilized gel

[0056] Add 0.8–1 part of ethylene glycol diglycidyl ether (EGDE) to a reaction flask, mix with filtered natural seawater, and place in a 40°C constant temperature water bath shaker at 120 rpm for 20 min to form an EGDE solution with a concentration of 0.8%–1.0%. Cool to room temperature. Place the coral skeleton with the gel beads already attached into the solution and let it stand for 30 min. The preparation of the barnacle-like coral regeneration gel is complete.

[0057] Compared with existing technologies, the beneficial effects of this invention are:

[0058] This invention proposes an innovative barnacle-like coral regeneration gel that utilizes biotechnology to restore coral reef ecosystems through optimized bio-adhesives and cross-linking techniques. Compared to traditional physical remediation methods, this bioremediation approach offers lower economic costs and higher ecological benefits, providing a cost-effective solution for the long-term protection and restoration of coral reefs.

[0059] First, this invention prepares gel beads primarily composed of propylene glycol alginate (PGA) and calcium chloride (CaCl2) to encapsulate coral larvae and zooxanthellae. These beads exhibit strong biocompatibility, ensuring normal growth of both coral larvae and zooxanthellae, promoting the establishment of their symbiotic relationship, and significantly improving their survival rate and growth rate. During this process, the Ca in the calcium chloride... 2+ It undergoes a cross-linking reaction with the carboxyl (-COOH) and hydroxyl (-OH) groups in PGA to form gel beads that encapsulate coral larvae and zooxanthellae.

[0060] Simultaneously, the 3,4-dihydroxyphenylalanine (DOPA) solution prepared in this invention, combined with the tyrosinase-catalyzed adhesion method, enables the gel beads to firmly adhere to the coral skeleton surface, forming a stable adhesion layer and providing a stable and safe growth and development space for corals and zooxanthellae. During this process, the hydroxyl (-OH) and carboxyl (-COOH) groups in DOPA can undergo esterification and hydrogen bonding reactions with the hydroxyl (-OH) and carboxyl (-COOH) groups in PGA, forming a stable cross-linked structure. Furthermore, DOPA undergoes a coordination reaction with calcium ions on the coral skeleton, further stabilizing the gel structure. Under the catalysis of tyrosinase, some DOPA is oxidized to dopaquinone, which can undergo a Michael addition reaction with unoxidized DOPA to form a cross-linked polydopamine (PDA) structure, providing a stable adhesion layer and enabling the gel beads to firmly adhere to the coral skeleton surface.

[0061] In addition, the addition of ethylene glycol diglycidyl ether (EGDE) allows EGDE to undergo a cross-linking reaction with unreacted PGA: the epoxy groups in EGDE react with the hydroxyl groups (-OH) in PGA to form ether bonds, which further enhances the mechanical strength and stability of the gel beads, enabling the prepared carrier to remain stable in a seawater environment.

[0062] The preparation method of this invention has clear steps, is simple to operate, and can be achieved under conventional laboratory conditions. Furthermore, although this invention uses only robust staghorn coral (Acropora robusta) larvae and zooxanthellae (Cladocopium goreaui), its method is applicable to the cultivation and regeneration of various corals and zooxanthellae, demonstrating broad applicability.

[0063] Testing revealed that the barnacle-like coral regeneration gel prepared in this invention possesses strong mechanical strength, ranging from 73 to 89 mN. It can effectively immobilize coral larvae and zooxanthellae in varying seawater environments with temperatures ranging from 16.2 to 37.4°C and pH levels from 6.7 to 9.1, achieving an efficiency of 80.87% to 95.72%. The bio-adhesive firmly adheres to the gel beads, preventing them from detaching in seawater waves. Furthermore, this carrier exhibits excellent biocompatibility; the immobilized coral larvae and zooxanthellae demonstrate good biological activity, with a relative biological activity approximately 65% ​​higher than that of the free state. Attached Figure Description

[0064] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0065] Figure 1 This is a flowchart of the preparation method of the present invention;

[0066] Figure 2 This is a gel image of the gel beads in which coral larvae and zooxanthellae were initially fixed in Example 1 of the present invention;

[0067] Figure 3 This is a schematic diagram illustrating the principle of PGA preparation in Example 1 of the present invention;

[0068] Figure 4 This is a schematic diagram illustrating the principle of DOPA preparation in Example 1 of the present invention. Detailed Implementation

[0069] The technical solution of the present invention will be further described in detail below with reference to examples.

[0070] Example 1

[0071] like Figure 1 As shown in this embodiment, a method for preparing a barnacle-like coral regeneration gel includes the following steps:

[0072] 1. Preparation of gel beads for preliminary fixation of coral larvae and zooxanthellae

[0073] (1) Prepare a culture medium containing robust staghorn coral (Acropora robusta) larvae and zooxanthellae (Cladocopium goreaui).

[0074] 300 ml of filtered natural seawater was added to L1 medium, along with 8 g of glucose. The mixture was stirred for 2 minutes, and then robust staghorn coral (Acropora robusta) and zooxanthellae (Cladocopium goreaui) were slowly added at 26°C. Natural conditions were simulated using a 12-hour light-12-hour dark photocycle, with a light intensity of 90 μE / m². 2 Under conditions of / s, maintain ventilation in the experimental environment and incubate for 1 day.

[0075] (2) Preparation of propylene glycol alginate (PGA) solution:

[0076] A. Esterification reaction

[0077] Add 2.8g of sodium alginate (SA) to the reaction flask, mix it with filtered natural seawater, place it on a 40℃ constant temperature water bath shaker and heat until it dissolves to form a 0.8% SA solution, then cool to room temperature.

[0078] 100 ml of filtered natural seawater, 200 ml of 0.8% SA solution, 13 g of propylene oxide, and finally 1 g of sodium hydroxide were added to a pressure reactor. The reactor was subjected to a working pressure of 10 bar and a temperature of 70°C for 3.5 h, and then cooled to room temperature.

[0079] B. Neutralization, extraction, and purification

[0080] After the esterification reaction, the product needs to be neutralized to prevent over-reaction or side reactions. Add an appropriate amount of acetic acid dropwise to the reaction vessel and use a pH meter to monitor the solution pH, adjusting it to 7.

[0081] Add 500 ml of 95% ethanol solution to the reactor and mix at 120 rpm for 20 min on a water bath shaker to precipitate PGA. Filter the product, dissolve the precipitate in a small amount of water, and cool at -10°C for 30 min to allow PGA crystallization. Filter a second time to further remove impurities.

[0082] C. Washing and drying:

[0083] Take a reaction flask, add 200 ml of water, add the PGA precipitate obtained in step B, add 30 g of methanol, stir for 5 min, and filter to remove unreacted starting materials and byproducts. Repeat this process 4 times.

[0084] The washed PGA was dried in a vacuum drying oven for 2 hours to remove residual solvent and moisture. After drying, the PGA formed a powdery solid.

[0085] D. Preparation of PGA solution

[0086] Dissolve 1.5g of PGA powder in water to form a 1.5% PGA solution.

[0087] (3) Preliminary cross-linking:

[0088] Take one reaction flask, add 12g of calcium chloride and 300ml of filtered natural seawater, and stir to form a 4% calcium chloride solution. In another reaction flask, take 50ml of culture medium containing robust staghorn coral larvae (Acropora robusta) and zooxanthellae (Cladocopium goreaui), and mix with 50ml of a 1.5% PGA solution. Using a pipette, add the mixture dropwise to the calcium chloride solution, allowing initial cross-linking to form gel beads. Let it stand for 40 minutes.

[0089] (4) Cleaning the gel beads:

[0090] Remove the gel beads and gently rinse them with deionized water to remove excess calcium chloride from the surface.

[0091] 2. Preparation and adhesion of bio-adhesives

[0092] (1) Preparation of 3,4-dihydroxyphenylalanine (DOPA) solution

[0093] A. Synthesis of 3-bromo-L-tyrosine

[0094] Take a reaction flask, add 48g of hydrogen bromide (HBr) and 100ml of water, and stir until HBr dissolves to obtain a 48% hydrogen bromide aqueous solution. Add 18g of L-tyrosine to the hydrogen bromide aqueous solution and place it in a 3°C constant temperature water bath shaker to mix at 120rpm for 2 hours.

[0095] The reaction flask was then removed, the solution was heated to room temperature, and placed in a 30°C constant temperature water bath shaker to mix at 120 rpm for 30 minutes.

[0096] Take 300 ml of cold water at 10°C, quickly mix the reaction solution with the cold water, and a solid will precipitate. Filter, and then wash the solid with cold water to obtain 3-bromo-L-tyrosine.

[0097] B. Hydroxylation reaction

[0098] Take 10g of 3-bromo-L-tyrosine obtained in step A, dissolve it in 100ml of filtered natural seawater, add 2g of sodium hydroxide and 0.5g of copper iodide (CuI), and place it in a 25℃ constant temperature water bath shaker at 120rpm for 3h. Filter, add acetic acid to neutralize the filtrate to pH=7.

[0099] Add 400 ml of 95% ethanol solution to the reaction solution to precipitate DOPA. Filter and dry in a vacuum drying oven for 2 hours to obtain purified DOPA.

[0100] The purified DOPA was dissolved in an appropriate amount of water to prepare a 5% DOPA solution.

[0101] (2) Soaking and coating:

[0102] At room temperature, take 30 ml of a 5% DOPA solution and immerse 15 g of cleaned gel beads in the solution for 5 minutes. Then remove the beads and simultaneously use a sterilized wool brush to apply the DOPA solution to the surface of a 5-10 cm section of coral skeleton. Ensure that the gel beads and coral skeleton are evenly covered with the DOPA solution.

[0103] (3) Adhesion:

[0104] At room temperature, gently place the soaked gel beads on the surface of the coral skeleton coated with DOPA solution, press the gel beads gently, and maintain pressure for about 1 to 2 minutes to ensure close contact between the gel beads and the coral skeleton surface, allowing DOPA to form a thin adhesive layer between the two surfaces.

[0105] (4) Curing DOPA:

[0106] Take a reaction flask, add 3g of tyrosinase and filtered natural seawater to form a 1% tyrosinase solution. Place the coral skeleton with the gel beads already attached into the solution, which will oxidize DOPA and accelerate the curing process of the DOPA adhesive. Remove it after standing for 1 hour.

[0107] 3. Synthesis of immobilized gel

[0108] Add 0.9g of ethylene glycol diglycidyl ether (EGDE) to a reaction flask and mix with 100ml of filtered natural seawater. Place the mixture in a 40℃ constant temperature water bath shaker and mix at 120rpm for 20min to form a 0.9% EGDE solution. Cool to room temperature. Place the coral skeleton with the gel beads already attached into the solution and let it stand for 30min. The preparation of the barnacle-like coral regeneration gel is complete.

[0109] 4. Testing the physicochemical properties of coral regeneration gel

[0110] The physicochemical properties of the synthesized coral regeneration gel, including adsorption capacity, specific surface area, mechanical strength, and immobilization efficiency, were tested. The test results are shown in Table 1.

[0111] Table 1. Test table of physicochemical properties of immobilized carriers

[0112]

[0113] Testing revealed that the barnacle-like coral regeneration gel prepared in this invention possesses strong mechanical strength, ranging from 73 to 89 mN. It can effectively immobilize coral larvae and zooxanthellae in varying seawater environments with temperatures ranging from 16.2 to 37.4°C and pH levels from 6.7 to 9.1, achieving an efficiency of 80.87% to 95.72%. The bio-adhesive firmly adheres to the gel beads, preventing them from detaching in seawater waves. Furthermore, this carrier exhibits excellent biocompatibility; the immobilized coral larvae and zooxanthellae demonstrate good biological activity, with a relative biological activity approximately 65% ​​higher than that of the free state.

[0114] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A barnacle-like coral regeneration gel, characterized in that, The coral regeneration gel is synthesized from gel beads that initially immobilize coral larvae and zooxanthellae, coral skeleton, bio-adhesive, and ethylene glycol diglycidyl ether. The bio-adhesive includes a 3,4-dihydroxyphenylalanine solution and a tyrosinase solution. The gel beads are placed on the surface of the coral skeleton coated with the 3,4-dihydroxyphenylalanine solution. The coral skeleton with the gel beads attached is then placed in the tyrosinase solution to solidify the 3,4-dihydroxyphenylalanine, and then placed in the ethylene glycol diglycidyl ether solution to obtain the barnacle-like coral regeneration gel.

2. The coral regeneration gel according to claim 1, characterized in that, The gel beads used to initially fix coral larvae and zooxanthellae are mainly composed of propylene glycol alginate and calcium chloride, which encapsulate coral larvae and zooxanthellae.

3. The coral regeneration gel according to claim 1, characterized in that, The bioadhesive comprises a 1-5% solution of 3,4-dihydroxyphenylalanine and a 1-5% solution of tyrosinase.

4. The coral regeneration gel according to claim 1, characterized in that, The initial fixation consisted of 2-6 parts of gel beads containing coral larvae and zooxanthellae, 1-3 parts of coral bone, 0.8-1.5 parts of biological adhesive, and 0.8-1 parts of ethylene glycol diglycidyl ether.

5. A method for preparing a barnacle-type coral regeneration gel according to any one of claims 1-4, wherein the gel beads are placed on the surface of a coral skeleton coated with a 3,4-dihydroxyphenylalanine solution, the coral skeleton with the gel beads already attached is placed in a tyrosinase solution to solidify the 3,4-dihydroxyphenylalanine, and then placed in an ethylene glycol diglycidyl ether solution to obtain the barnacle-type coral regeneration gel.

6. The method for preparing a barnacle-like coral regeneration gel according to claim 5, characterized in that, The preparation method includes the following steps: 1) Take the culture medium of coral larvae and zooxanthellae, mix it with propylene glycol alginate solution, and add the mixed solution dropwise to calcium chloride solution to initially crosslink and form gel beads; 2) Place the gel beads on the surface of coral skeleton coated with 3,4-dihydroxyphenylalanine solution to ensure close contact between the gel beads and the coral skeleton surface; 3) Put the coral skeleton with the gel beads attached into tyrosinase solution to solidify 3,4-dihydroxyphenylalanine; 4) Then put it into ethylene glycol diglycidyl ether solution to obtain barnacle-like coral regeneration gel.

7. The method for preparing a barnacle-like coral regeneration gel according to claim 6, characterized in that, The concentration of the propylene glycol alginate solution is 1-2%.

8. The method for preparing a barnacle-type coral regeneration gel according to claim 5, characterized in that, The concentration of the ethylene glycol diglycidyl ether solution is 0.8% to 1.0%.

9. The application of a barnacle-type coral regeneration gel according to any one of claims 1-4, wherein the coral regeneration gel is used to promote coral regeneration.

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