Large-scale fries breeding method for hedge-festival snails

Abstract

The invention belongs to the technical field of large-scale shellfish fry cultivation, and discloses a large-scale fries cultivation method for hedge fries. In combination with actual breeding experience, the main reason of seedling breeding failure is that the seedling breeding water body cannot develop to the seedling stage of benthic life due to the problems that the death rate is high in the floating stage and the attachment stage, the seedling breeding water body is prone to being invaded by protozoa, and harmful algae in the water body are prone to outbreak and the like. According to the method, by optimizing the seedling raising environment, improving the seedling raising technology and controlling the illumination, the bottleneck of the hedge snail seedling raising is successfully broken through, the hedge snail seedlings can be cultivated on a large scale, the juvenile snails which are 1-2 cm in specification and suitable for enhancement and releasing or artificial breeding are provided, and a solid reference is provided for breeding of the hedge snails.

Description

Technical Field

[0001] The invention belongs to the technical field of large-scale shellfish seedling cultivation, and more specifically, relates to a large-scale seedling cultivation method for Epipremnum aquatica. Background Art

[0002] Strombus luhuanus, commonly known as red-mouthed snail or strawberry snail, is named for the orange-red color of the inside of its shell. It belongs to the class Gastropoda, order Mesogastropoda, family Strombidae, and genus Strombus. It lives in the intertidal zone to the bottom of shallow sea sand, gravel or coral reefs.

[0003] In recent years, due to the decline of natural populations, deterioration of coastal ecological environment, habitat destruction and over-fishing by humans, the number of water snails has dropped sharply, seriously threatening the survival and reproduction of water snails.

[0004] In order to protect ecological diversity and ensure the population size of the hedge snail, it is urgent to develop the seedling technology of the hedge snail, which is of great significance to the resource recovery, reproduction and release, and artificial breeding of the hedge snail. Although many scholars have explored the seedling cultivation of the hedge snail, there has been no report on the successful cultivation of the hedge snail seedlings so far. Summary of the invention

[0005] In order to make up for the deficiencies in the prior art, the present invention proposes a method for large-scale seedling cultivation of Pteris viridis.

[0006] The second object of the present invention is to provide application of the above method.

[0007] The purpose of the present invention is achieved through the following technical solutions:

[0008] A method for large-scale cultivation of larvae, comprising the following steps:

[0009] (1) Cultivation of algae bait: After cleaning and disinfecting the algae attachment plate, immerse it in a running water pool for 2 to 3 times, and when the algae attachment plate turns dark brown, place it in a static culture for later use;

[0010] The nano-algae operation is as follows: placing the algae attachment plate in running water for 2 to 3 days, and then washing the algae attachment plate with seawater;

[0011] (2) Construction of a flowing water aquaculture pond: Select a aquaculture pond with moderate light, clean and disinfect it, and then immerse a 300-mesh screen bag with an open top into the aquaculture pond. Set a water flow system and a feeding system in the screen bag to form an independent aquaculture water environment in the screen bag;

[0012] (3) Cultivation of planktonic larvae of snail seedlings: Place the egg bag of the snail on a basket pre-placed with an 80-mesh screen, so that the basket floats in the culture water in the screen bag, and then cover the entire culture pond with canvas for light-shading culture. After 1 to 2 days, planktonic larvae appear in the culture water in the screen bag. Start feeding the planktonic larvae with live bait algae until they develop to the late stage of the veliger larvae and wait for attachment and metamorphosis;

[0013] (4) Attachment and metamorphosis of snail seedlings: During the attachment and metamorphosis of snail seedlings, open the shade canvas, stop feeding live algae as bait, string together 4 to 5 algae attachment plates prepared in step (1) with ropes, and hang them in the mesh bag culture water body of the planktonic larvae of snail seedlings that have developed to the late stage of the veliger larvae obtained in step (3), and cultivate them until snail seedlings with a size of 1 to 3 mm can be seen attached to the algae attachment plates with the naked eye;

[0014] (5) Snail seedling rearing: The algae attachment plates with attached snail seedlings are disassembled into pieces, spread flat on the bottom of the breeding pond, and fed with bait. After 60 to 90 days of rearing, the snail seedlings will grow into young snails with a size of 10 to 18 mm. At this time, the young snails can be peeled off and reared in the breeding pond.

[0015] At present, only a few scholars have reported the growth of hedge snail seedlings in the wild environment. Although some scholars have explored the artificial cultivation of hedge snail seedlings, the longest survival of planktonic larvae is only 17 days, and they have not even developed to the attachment metamorphosis stage. Therefore, there is no successful case of hedge snail seedling cultivation. Early exploratory experiments found that the bottlenecks of hedge snail seedling cultivation are the rapid growth of harmful algae and protozoa causing the death of planktonic larvae, the low survival rate of attachment metamorphosis, and the high water quality requirements.

[0016] 1. In order to inhibit the rapid growth of harmful algae and protozoa, the present invention utilizes the fact that algae and protozoa are difficult to grow in a dark environment, completely shades the culture pond, and cultivates the planktonic larvae of the snail seedlings, effectively solving the problem of snail seedling death caused by the rapid growth of harmful organisms.

[0017] 2. Different from the attachment metamorphosis conditions of scraping shellfish such as Dongfeng conch and abalone, the larvae of the water chestnut prefer to feed on firmly attached diatoms, but the thin film algae method used by abalone cannot capture firmly attached diatoms, and the floating nature of the film is not conducive to the attachment of the water chestnut seedlings. Therefore, the present invention adopts the attachment plate algae method, removes the loosely attached algae by multiple flushing, efficiently captures a sufficient amount of diatoms, and solves the bottleneck problem of low attachment metamorphosis rate.

[0018] 3. Unlike the water exchange cultivation mode used in the breeding ponds of shellfish such as oysters, abalone, scallops, and baby snails, the snails are very sensitive to changes in water quality and will die in large numbers during water exchange. Therefore, we use the screen-in-water breeding mode to completely solve the problem of abnormal death of snails caused by unstable water quality.

[0019] Preferably, in the above-mentioned method for large-scale seedling cultivation of P. fasciatus, the operation of step (3) of feeding the planktonic larvae with live bait algae is as follows:

[0020] S1. After the planktonic larvae appear in the mesh bag, start feeding golden algae bait, feeding once in the morning, noon and evening every day, each time with a density of 10,000 to 20,000 golden algae per milliliter; turn off the water flow system and the feeding system during feeding, and turn on the water flow system and the feeding system again after 1 hour of feeding;

[0021] S2. After 5-10 days of culture of planktonic larvae, feed them with a mixture of golden algae, horn algae, chlorella and flat algae at a feeding density of 20,000 to 30,000 per milliliter;

[0022] S3. After 20 to 25 days of culture, the planktonic larvae will develop into the late stage of the veliger larvae.

[0023] Preferably, in the above-mentioned method for large-scale cultivation of Epipremnum aureum seedlings, in step (4), 2 to 3 strings of algae attachment plates are hung per cubic meter of water, and the algae attachment plates are cultured in flowing water for 7 to 10 days.

[0024] Another key issue that determines the success of the cultivation of water snail seedlings is the feeding of young snail seedlings. We have successfully found that young snails like to eat algae. By feeding them with algae such as Thalassiosira and Enteromorpha, we ensure the normal growth and development of young snails and their high survival rate. We have successfully solved the problem of feeding water snail seedlings and provided a solid foundation for the artificial and large-scale breeding of water snails.

[0025] Therefore, preferably, in the above-mentioned method for cultivating large-scale seedlings of P. fasciatus, step (5) is to promote the growth of the snail seedlings by adding 2 to 3 pinches of algae food to each corrugated plate, and more can be added after the snail seedlings have finished eating. The algae food is Streptospira and Enteromorpha.

[0026] The present invention also provides application of the large-scale cultivation method of water chestnut seedlings in large-scale cultivation of water chestnuts.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The present invention consults the data and combines the actual breeding experience to draw the conclusion that the main reasons for the failure of seedling cultivation are high mortality in the two stages of floating and attachment, the seedling water body is easily invaded by protozoa, and harmful algae in the water body are prone to outbreaks, resulting in the inability to develop to the benthic seedling stage. The present invention successfully breaks through the bottleneck of the cultivation of hedge snails by optimizing the seedling environment, improving the seedling technology, and controlling the light. It can cultivate hedge snail seedlings on a large scale, provide juvenile snails with a size of 1 to 2 cm, suitable for proliferation, release or artificial breeding, and provide a solid reference for the breeding of hedge snails. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1The following is the situation of algae on the corrugated board for 3 days; (A) A large amount of sticky debris is attached to the corrugated board before flushing; (B) The algae on the corrugated board before flushing is observed under a microscope, showing that there are many debris; (C) After flushing, the corrugated board is clean, with few debris and yellow-brown in color; (D) The algae on the corrugated board after flushing is observed under a microscope, and is evenly distributed, with various algae such as rhombus algae and diatoms;

[0030] Figure 2 This is the corrugated plate nanoalgae after 20 days; (A) front view; (B) back view;

[0031] Figure 3 This is a flow-through culture system for planktonic larvae; (A) water inlet outside the screen (long arrow), water inlet inside the screen (short arrow), and water flowing through the PVC pipe with holes drilled just above the screen (dashed arrow); (B) air-filled tube inside the screen (long arrow);

[0032] Figure 4 It is the hatching and cultivation of planktonic larvae; (A) The basket containing the egg sac floats on the water surface (long arrow), and the eggs will swim into the screen after breaking the membrane; (B) The snail fry breeding pond is shaded by canvas during the breeding process;

[0033] Figure 5 This is the egg bag of the hedge snail; (A) The egg bag (long arrow) laid next to the parent snail is stuck together and fluffy; (B) The egg bag peeled off from the spawning pond; (C) A single egg bag (long arrow), filamentous, light yellow, about 1mm in diameter; (D) The larvae in the egg bag (long arrow), about 150 microns in diameter, are in the blastocyst stage;

[0034] Figure 6 This is the development process of the snail from 1 to 4 days after hatching; (A) On the first day, the eyespot, yolk sac (arrow) and rotational movement appear; (B) On the second day, the body begins to twist; (C) On the third day, it begins to eat, and food (arrow) appears in the stomach; (D) On the fourth day, the larvae begin to swim straight ahead;

[0035] Figure 7 This is the development process of the larvae of the hedge snail for 5 to 8 days; (A) On the fifth day, the larvae have tentacles (arrows); (B) On the sixth day, the larvae's internal organs (arrows) are clearly visible, including the hepatopancreas, digestive glands, etc.; (C) On the seventh day, the larvae's body whorl is obvious (arrow); (D) On the eighth day, the larvae's velum (arrow) develops further, becomes four-leafed, and can be retracted into the body whorl;

[0036] Figure 8This is the development process of the larvae of the hedge snail from 9 to 16 days; (A) On the ninth day, the shell of the larva is obvious, and the velum can be completely retracted into the shell; (B) On the eleventh day, the larva has obvious tentacles (arrows), and the velum extends over a large area; (C) On the fifteenth day, the larva is cotton-like (arrows) and can be seen with the naked eye; (D) On the sixteenth day, the larva has a mouth and tongue (arrows), and the velum is six-lobed;

[0037] Fig. 9 This is the development process of the larvae of the hedge snail from 17 to 22 days; (A) On the 17th day, the larvae have ventral feet (arrows) and slender tentacles (dashed arrows); (B) On the 18th day, the larvae have opercula (arrows); (C) On the 20th day, most larvae have fully developed and the cilia have basically disappeared; (D) On the 22nd day, the larvae enter the creeping stage and are ready to attach;

[0038] Fig.10 This is the development process of a juvenile snail; (A) 9 days after attachment, the mouth, tongue and tentacles of the juvenile snail (arrow) are visible to the naked eye; (B) 19 days after attachment, the juvenile snail (arrow) is attached to the stone and scrapes the algae on the stone; (C) 19 days after attachment, the shell of the juvenile snail is about 4 mm high; (D) 39 days after attachment, a juvenile snail is partially peeled off; (E) 39 days after attachment, the shell of the juvenile snail is about 13 mm high; (F) 69 days after attachment, the shell of the juvenile snail is about 17 mm high. DETAILED DESCRIPTION

[0039] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with specific drawings and embodiments. In the embodiments, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0040] Example 1

[0041] 1. Cultivation of algae bait

[0042] Corrugated board to remove benthic diatoms (nanoalgae): clean the corrugated board and use strong chlorine (concentration 20-40g / m 3 ) or potassium permanganate (concentration 5-15 g / m 3 ) Soak for 30 to 60 minutes and set aside.

[0043] Immerse the corrugated board in a flowing water pool with a water depth of 0.5 to 1m and good light. After leaving it for 2 to 3 days, check the algae content of the corrugated board. Figure 1 A and Figure 1As shown in B, a lot of sticky debris adheres to the corrugated board in the first 2 to 3 days, and many protozoa shuttle across the board surface. At this time, the corrugated board needs to be rinsed with seawater to wash away the impurities and protozoa on the board, leaving the firmly attached diatoms as algae species. Place it out of water for 2 to 3 hours to try to let the protozoa die from drying. After treatment, the attached board is obviously clean and yellow-brown, and the algae are firmly attached. Observation under a microscope shows that the algae are evenly distributed and there are many species, including rice-grain-shaped diamond-shaped algae, chain-bead-shaped sea algae, and other algae ( Figure 1 C, D).

[0044] To ensure that there are enough algae species, the corrugated board after washing is immersed in the flowing water pool for further cultivation, and then the algae are placed 2 to 3 times or even more times. After multiple cultivation and washing steps, algae food with high purity and sufficient quantity can be obtained in about 20 days. At this time, the corrugated board is dark brown and about 1mm thick ( Figure 2 ).

[0045] Add diatom culture nutrient salts to the algae culture pond, that is, add 15g urea, 5g potassium dihydrogen phosphate, 0.05g ferric citrate, and 10g sodium silicate per cubic meter of seawater. Maintain proper lighting, a water depth of 0.5-1m, and oxygenation in the algae culture pond, so that the dark brown corrugated plate is left standing for use.

[0046] 2. Construction of flow-through aquaculture system

[0047] Choose a cement pool with moderate light (size: [length 5-8m*width 2-4m]), clean the bottom and walls of the pool, and apply strong chlorine (concentration 20-40g / m 3 ) or potassium permanganate (concentration 5-15 g / m 3 ) for overnight disinfection.

[0048] A 3m*3m long, 300 mesh (aperture of about 50μm) screen was customized and fixed in the middle of the culture pond with a thick bamboo pole. A PVC pipe (diameter 15mm) was built, spanning the long side of the culture pond in the middle. The PVC pipe above the screen was punched with holes at intervals of 10cm. One end of the PVC pipe was connected to the water inlet, and a valve was installed at the other end, forming a simple water flow system ( Figure 3 A). Place air stones evenly above the bottom of the screen, about 30 cm from the bottom, to form an oxygen supply system ( Figure 3 B). Open the water inlet of the breeding pond, the water flow rate is 3-10L / min, and the water depth of the outlet is controlled to 1.2m, forming a water flow system in the breeding pond. Open the water inlet of the PVC pipe, the water flow rate is 2-5L / min, close the valve at the other end, forming a water flow system in the screen. Open the oxygen supply system, maintain the gas output of the aerated stone, and form an oxygen supply system in the screen.

[0049] 3. Egg Bag

[0050] The egg sacs of the hedge snails are usually laid between pairs or groups of 3 to 5 parent snails. They are stuck together in a pile and fluffy. The color is similar to that of the environment. The egg sacs laid in the sand are brownish yellow in color. The egg sacs are often connected into a piece, about 2 to 5 cm long. A single egg sac is filamentous, light yellow, about 1 mm in diameter, and 3 to 10 cm in length. There are a large number of larvae in the blastocyst stage or single-wheel larval stage in the egg sacs. The larvae can be seen rotating in the membrane and have not yet broken the membrane ( Figure 5 ).

[0051] 4. Snail fry planktonic larvae culture

[0052] The egg bags of the snails are placed on a basket with an 80-mesh screen and foam tied around it, and then the basket is floated in the flowing water culture pond. 200 to 300 egg bags are placed in each culture pond. In order to avoid or delay the growth of harmful algae and protozoa, the entire flowing water culture pond is covered with canvas to block light during the whole process of larval plankton culture ( Figure 4 B). After 1 to 2 days, the fertilized eggs mature in the egg sac, break the membrane and form planktonic larvae. After the planktonic larvae appear in the screen, start feeding golden algae bait, feeding once a day in the morning, noon and evening, each time with a density of 10,000 to 20,000 golden algae per milliliter. Turn off the convection water and oxygen supply equipment during feeding, and turn on the convection water and oxygen supply equipment again after 1 hour of feeding. After 5 to 10 days of culture of planktonic larvae, feed a mixture of golden algae, Chaetoceros algae, Chlorella vulgaris, and Platymorpha (no limit on the proportion), with a feeding density of 20,000 to 30,000 per milliliter. After 20 to 25 days of culture of planktonic larvae, they can develop to the late stage of the veliger larvae and wait for attachment and metamorphosis.

[0053] The morphological changes of the planktonic larvae are as follows:

[0054] Day 1: The larvae break out of the egg sac and start to swim in a rotation, with a diameter of about 150um. Eye spots appear, two tufts of cilia on the head, and a yolk sac at the rear end of the body ( Figure 6 A).

[0055] Day 2: The larvae begin to develop and twist slightly, with rotational movements as the main feature ( Figure 6 B).

[0056] Day 3: Larvae begin to eat algae, digestive glands appear, and food is clearly present in the stomach ( Figure 6 C).

[0057] Day 4: The larvae begin to change from rotating to straight swimming, the preoral organs continue to develop, and a clear ciliary wheel appears ( Figure 6 D).

[0058] Day 5: The larva is about 200um in diameter, tentacles begin to appear, and the body further twists ( Figure 7 A).

[0059] Day 6: The larvae's internal organs develop further, with digestive glands and hepatopancreas becoming more prominent ( Figure 7 B).

[0060] Day 7: The larva has the rudiments of an adult snail, with obvious body whorls and a shorter spiral part ( Figure 7 C).

[0061] Day 8: The larval veliger develops further and becomes four-lobed, and the veliger can be retracted into the body whorl ( Figure 7 D).

[0062] Day 9: The larva is about 300um, with a distinct shell, enlarged body whorls, and the face plate can be completely retracted into the shell ( Figure 8 A).

[0063] Day 11: Larvae have obvious tentacles and mostly swim in the middle and lower water layers ( Figure 8 B).

[0064] Day 15: The larvae are about 400um in size, cotton-like and visible to the naked eye ( Figure 8 C).

[0065] Day 16: The larvae develop a mouth and tongue, and begin to switch from filter feeding to scraping algae. The facial disc becomes six-lobed ( Figure 8 D).

[0066] Day 17: The larvae have developed abdominal feet, their tentacles have become slender, and their shells are fully developed with obvious tops ( Fig. 9 A).

[0067] Day 18: The larvae have an operculum, their ventral feet become thicker, and their contraction ability is strong ( Fig. 9 B).

[0068] Day 20: The larvae's facial disc begins to degenerate, and the cilia basically disappear ( Fig. 9 C).

[0069] Day 22: The larva is about 500um, enters the crawling stage, and the gastropod is fully developed, ready to attach ( Fig. 9 D).

[0070] 5. Snail seedling attachment metamorphosis

[0071] During the period of metamorphosis and attachment of snail seedlings, open the shade canvas covering the breeding pond. Stop feeding live algae. Use ropes to string 4 to 5 pieces of corrugated boards that have been inoculated with algae for more than 20 days in advance, and then hang them in the breeding pond for snail seedlings and planktonic larvae that have developed to the late stage of the disc larvae. Hang 2 to 3 strings of corrugated boards per cubic water body. After 7 to 10 days of flowing water cultivation, snail seedlings of 1 to 3 mm in size can be seen attached to the corrugated boards with the naked eye.

[0072] 6. Snail seedling rearing

[0073] Remove the corrugated board with attached snail seedlings into pieces and lay them flat on the bottom of the culture pond. To promote the growth of snail seedlings, add 2 to 3 pinches of algae such as Thalassiosira and Enteromorpha to each corrugated board, and continue to add algae after the snail seedlings have finished feeding. Continue to feed in the culture pond for 60 to 90 days, and the snails will grow to a size of 10 to 18 mm. At this time, the snails on the corrugated board can be peeled off and raised in the culture pond. The young snails mainly feed on algae on the bottom or wall of the culture pond. When food is scarce, add algae such as Thalassiosira and Enteromorpha to the culture pond.

[0074] The morphological observations of the juvenile snails are as follows:

[0075] Day 30 (9 days after attachment): Juvenile snail shell is about 2mm high, fully developed, with mouth, tongue, tentacles and other organs clearly visible, scraping diatoms on the corrugated board ( Fig.10 A).

[0076] Day 40 (19 days after attachment): The shell of the young snail is about 4mm high. It likes to attach to hard materials such as stones and sand. It is sensitive and will shrink immediately after being frightened. It has a strong ability to flip over ( Fig.10 B, C).

[0077] Day 60 (39 days after attachment): Juvenile snails have shells about 13 mm high and feed similarly to adults, such as large algae such as Thalassiosira and Enteromorpha. Their shells are thicker, their body color turns brown, their whorls become harder, and their spirals are more obvious. Fig.10 D, E).

[0078] Day 90 (69 days after attachment): The shell height of the young snail is about 17mm, the growth rate is slower than before, the shell becomes thicker, the activity is stronger, and the range is wider ( Fig.10 F).

[0079] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A method for large-scale cultivation of seedlings of Pteris viridis, characterized in that: The following steps are involved: (1) Cultivation of algae bait: After cleaning and disinfecting the algae attachment plate, immerse it in a running water pool for 2 to 3 times, and when the algae attachment plate turns dark brown, place it in a static culture for later use; The nano-algae operation is as follows: placing the algae attachment plate in running water for 2 to 3 days, and then washing the algae attachment plate with seawater; (2) Construction of a flowing water aquaculture pond: Select a aquaculture pond with moderate light, clean and disinfect it, and then immerse a 300-mesh screen bag with an open top into the aquaculture pond. Set a water flow system and a feeding system in the screen bag to form an independent aquaculture water environment in the screen bag; (3) Cultivation of planktonic larvae of snail seedlings: Place the egg bag of the snail on a basket pre-placed with an 80-mesh screen, so that the basket floats in the culture water in the screen bag, and then cover the entire culture pond with canvas for light-shading culture. After 1 to 2 days, planktonic larvae appear in the culture water in the screen bag. Start feeding the planktonic larvae with live bait algae until they develop to the late stage of the veliger larvae and wait for attachment and metamorphosis; (4) Attachment and metamorphosis of snail seedlings: During the attachment and metamorphosis of snail seedlings, open the shade canvas, stop feeding live algae as bait, string together 4 to 5 algae attachment plates prepared in step (1) with ropes, and hang them in the mesh bag culture water body of the planktonic larvae of snail seedlings that have developed to the late stage of the veliger larvae obtained in step (3), and cultivate them until snail seedlings with a size of 1 to 3 mm can be seen attached to the algae attachment plates with the naked eye; (5) Snail seedling rearing: The algae attachment plates with attached snail seedlings are disassembled into pieces, spread flat on the bottom of the breeding pond, and fed with bait. After 60 to 90 days of rearing, the snail seedlings will grow into young snails with a size of 10 to 18 mm. At this time, the young snails can be peeled off and reared in the breeding pond.

2. The method for large-scale seedling cultivation of Pteris fasciatus according to claim 1, characterized in that: The operation of step (3) feeding the planktonic larvae with live bait algae is as follows: S1. After the planktonic larvae appear in the mesh bag, start feeding golden algae bait, feeding once in the morning, noon and evening every day, each time with a density of 10,000 to 20,000 golden algae per milliliter; turn off the water flow system and the feeding system during feeding, and turn on the water flow system and the feeding system again after 1 hour of feeding; S2. After 5-10 days of culture of planktonic larvae, feed them with a mixture of golden algae, horn algae, chlorella and flat algae at a feeding density of 20,000 to 30,000 per milliliter; S3. After 20 to 25 days of culture, the planktonic larvae will develop into the late stage of the veliger larvae.

3. The method for large-scale seedling cultivation of Epipremnum aquatica according to claim 1, characterized in that: Step (4) 2 to 3 strings of algae attachment plates are hung per cubic meter of water, and the algae attachment plates are cultured in flowing water for 7 to 10 days.

4. The method for large-scale seedling cultivation of Epipremnum aquatica according to claim 1, characterized in that: Step (5) To promote the growth of the snail seedlings, 2 to 3 pinches of algae food are added to each corrugated plate, and more can be added after the snail seedlings have finished eating.

5. The method for large-scale seedling cultivation of Epipremnum aquatica according to claim 4, characterized in that: The algae food is Thalassiosira salina and Enteromorpha prostrata.

6. Use of the large-scale seedling cultivation method of Hercules snails according to any one of claims 1 to 5 in large-scale cultivation of Hercules snails.

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