A gas phase liquid nitrogen biological storage tank and use method thereof

Abstract

The present invention relates to the field of liquid nitrogen storage technology, and discloses a gas phase liquid nitrogen biological storage tank and a method of using the same, including a main body, the main body including a placement tank arranged inside the main body, a sealing cover 2 being slidably connected to the top of the placement tank, a sealing cover 1 being fixedly connected to the outer surface of the placement tank, an inner wall of the sealing cover 1 being slidably connected to the outer surface of the main body, and a fixed block being fixedly connected to one end of the sealing cover 1 away from the main body. In the present invention, when the gas phase liquid nitrogen enters the spiral tube, it will circulate inside the spiral tube and play a role in circulating and cooling the biological stored inside the placement tank, and by making the gas phase liquid nitrogen flow in the spiral tube, the material placed inside the spiral tube can be evenly cooled, and the uniform distribution of temperature can be better achieved, thereby improving the storage quality of the sample during storage.

Description

Technical Field

[0001] The invention relates to the technical field of liquid nitrogen storage, and in particular to a gas phase liquid nitrogen biological storage tank and a use method thereof. Background Art

[0002] Liquid nitrogen refers to liquid nitrogen, which is an extremely low temperature liquid. At room temperature, the boiling point of liquid nitrogen is -196.56°C. Liquid nitrogen does not react with most metals or oxygen, and is often used to store biological samples, such as cells, tissues, and bacteria. In some basic experiments and clinical practice, embryo vitrification technology can effectively prevent sample deterioration and microbial growth due to the ultra-low temperature characteristics of liquid nitrogen. Therefore, storage tanks filled with liquid nitrogen are usually used to store biological samples; Generally, existing gas-phase liquid nitrogen biological storage devices place liquid nitrogen at the bottom of the storage tank and place biological samples at the top of the storage tank. The liquid nitrogen at the bottom will continue to evaporate, change from liquid to gas and flow to the top of the storage tank to freeze and protect the biological samples. However, this method may cause temperature differences between different heights. The temperature is lower at locations close to the liquid nitrogen and higher at locations far from the liquid nitrogen, which may affect the storage quality of the samples. Summary of the invention

[0003] The object of the present invention is to provide a gas phase liquid nitrogen biological storage tank and a method of using the same to solve the problems raised in the above background technology.

[0004] In order to solve the above technical problems, the present invention is achieved through the following technical solutions: The present invention is a gas phase liquid nitrogen biological storage tank, comprising a main body, the main body comprising a placement tank arranged inside the main body, a sealing cover 2 is slidably connected to the top of the placement tank, a sealing cover 1 is fixedly connected to the outer surface of the placement tank, the inner wall of the sealing cover 1 is slidably connected to the outer surface of the main body, an end of the sealing cover 1 away from the main body is fixedly connected to a fixed block, a plurality of placement racks are slidably connected to the inside of the placement tank, a circular plate 1 is fixedly connected to the inside of the main body, a delivery pipe is fixedly connected to the outer surface of the main body, the end of the delivery pipe close to the main body passes through the inner wall of the main body and extends to the inside, a plurality of arc grooves are opened on the inner wall of the main body, and further comprising; The auxiliary mechanism includes a motor fixedly connected to the bottom of the placement tank, an end of the motor away from the placement tank is fixedly connected to a rotating column, an arc-shaped ring groove is provided on the outer surface of the rotating column, a plurality of short columns are slidably connected inside the arc-shaped ring groove, an end of the plurality of short columns away from the rotating column is fixedly connected to a hollow cylinder, an end of the hollow cylinder away from the motor is fixedly connected to a plurality of fixed plates, an end of the fixed plate close to the motor is fixedly connected to a telescopic spring column, and an end of the fixed plate away from the telescopic spring column is fixedly connected to a fixed long column.

[0005] Furthermore, a circular barrel is provided at the bottom of the hollow cylinder, and the circular barrel is slidably connected to the outer surface of the rotating column, and a swinging disc is fixedly connected to the side of the circular barrel away from the hollow cylinder, and the top of the swinging disc is fixedly connected to a plurality of fixed long columns, and a plurality of fixed spring plates are fixedly connected to the side of the swinging disc away from the hollow cylinder, and the side wall of the fixed spring plate is fixedly connected to the inner wall of the main body, and a plurality of spring plates are fixedly connected to the side of the swinging disc away from the hollow cylinder, and one end of the spring plate away from the motor is fixedly connected to the inner wall of the main body, and one end of the rotating column away from the motor is fixedly connected A circular plate is connected, and one end of several telescopic spring columns close to the motor is fixedly connected to a swing plate, and a side of the swing plate away from the motor is provided with several fixed cylinders, the outer surface of the fixed cylinder is fixedly connected to a C-shaped plate, the outer surface of the fixed cylinder located inside the C-shaped plate is fixedly connected to a fixed short plate, the end of the fixed short plate away from the fixed cylinder is rotatably connected to the rotating plate, the end of the fixed cylinder away from the swing plate is fixedly connected to a spring, the end of the fixed cylinder away from the fixed short plate is slidably connected to the inside of the arc groove, and the end of the spring away from the fixed cylinder is fixedly connected to the bottom of the arc groove.

[0006] Furthermore, a circulation mechanism is provided on the outer surface of the circular plate 1, and the circulation mechanism includes a spiral tube fixedly connected to the top of the circular plate 1, an end of the spiral tube away from the circular plate 1 is fixedly connected to a hollow block, a bottom of the hollow block is fixedly connected to a hollow tube, and an end of the hollow tube away from the hollow block is fixedly connected to a flexible hollow tube; The spiral tube is connected to the circular plate, and a one-way valve is fixedly connected inside the spiral tube.

[0007] Furthermore, a cooling mechanism is provided on the outer surface of the swinging disc, and the cooling mechanism includes two hollow telescopic columns rotatably connected to the inside of the swinging disc, one of the hollow telescopic columns is connected to the flexible hollow tube, a square sliding groove is provided on the outer surface of one of the hollow telescopic columns, and an airbag is fixedly connected to one end of the hollow telescopic column away from the swinging disc, and the two airbags are connected.

[0008] Furthermore, a fixing plate 2 is fixedly connected to the inner wall of one of the hollow telescopic columns, an end of the fixing plate 2 close to the airbag is rotatably connected to a bidirectional threaded rod, an outer surface of the bidirectional threaded rod is rotatably connected to a fixing short column, an end of the fixing short column away from the bidirectional threaded rod is fixedly connected to a fixing plate 3, an end of the fixing plate 3 away from the fixing short column passes through the square sliding groove and extends to the outside, and an end of the fixing plate 3 away from the fixing plate 2 is fixedly connected to the outer surface of the hollow telescopic column.

[0009] Furthermore, a plurality of inclined plates are fixedly connected to the outer surface of the bidirectional threaded rod, a return spring is arranged on the outer surface of the bidirectional threaded rod, one end of the return spring away from the inclined plate is fixedly connected to a limit plate, and the limit plate is fixedly connected to the inner wall of the hollow telescopic column.

[0010] Furthermore, a rotating mechanism is provided on the outer surface of the bidirectional threaded rod, and the rotating mechanism includes a rotating long column fixedly connected to the bottom of the bidirectional threaded rod, and the end of the rotating long column away from the bidirectional threaded rod penetrates the inner wall of the hollow plate and extends to the interior, the interior of the rotating long column is hollow, and two air inlet holes are provided on the outer surface of the rotating long column, and the end of the rotating long column away from the limit plate is fixedly connected to the hollow plate, and a plurality of L-shaped hollow columns are fixedly connected to the outer surface of the hollow plate.

[0011] Furthermore, the L-shaped hollow column is made of flexible material, and a hollow circle is fixedly connected to one end of the L-shaped hollow column away from the hollow plate. A plurality of exhaust holes are provided on the outer surface of the hollow circle, and a plurality of springs are fixedly connected to the outer surface of the hollow plate. The end of the spring away from the hollow plate is fixedly connected to the L-shaped hollow column.

[0012] Furthermore, a method for using a gas phase liquid nitrogen biological storage tank, the gas phase liquid nitrogen biological storage tank, the method comprising the following steps: S1: Open the sealing cover 1; S2: Place the sample; S3: transport liquid nitrogen; S4: Cooling storage.

[0013] The present invention has the following beneficial effects: 1. The present invention first uses a delivery pipe to deliver liquid nitrogen to the inside of the main body, and then starts the motor. When the motor works, it drives the rotating column to rotate. When the rotating column rotates, it drives the hollow cylinder to swing up and down through the arc-shaped ring groove and a plurality of short columns. When the hollow cylinder swings up and down, it drives the swing disc to swing up and down through a plurality of fixed long columns. When the swing disc swings up and down, the gaseous liquid nitrogen at the bottom of the main body will flow to the top of the swing disc through the circular barrel. When the hollow cylinder swings up and down, it drives the swing plate to swing up and down through a plurality of telescopic spring columns. When the swing plate swings up and down, it drives the fixed short plate and the fixed cylinder to swing up and down through the C-shaped plate. When the fixed short plate swings up and down, it will drive the rotating plate to swing up and down. When the fixed cylinder swings up and down, it will swing left and right under the action of the arc groove. When the swinging disc swings up and down, the gaseous liquid nitrogen at the top of the swinging disc will be under pressure and will enter the inside of the spiral tube from the one-way valve. After the gaseous liquid nitrogen enters the spiral tube, it will circulate inside the spiral tube and play a role in circulating and cooling the organisms stored in the tank. By making the gaseous liquid nitrogen flow in the spiral tube, the material placed inside the spiral tube can be evenly cooled, which can better achieve uniform temperature distribution, thereby improving the storage quality of the sample during storage.

[0014] 2. In the present invention, after the gas phase liquid nitrogen enters the spiral tube, it flows into the interior of the flexible hollow tube through the hollow block and the hollow tube. After the gas phase liquid nitrogen enters the interior of the flexible hollow tube, it flows into the interior of the hollow telescopic column. When the swinging disc swings up and down, the two air bags will also swing up and down together. When the swinging disc moves downward, the air bags float on the surface of the liquid nitrogen, so they will push the hollow telescopic column to contract. When the hollow telescopic column contracts, the fixed plate three and the fixed short column will slide on the outer surface of the bidirectional threaded rod. Since the fixed plate three is fixedly connected to the hollow telescopic column, the bidirectional threaded rod will rotate at this time. When the bidirectional threaded rod rotates, it will drive a plurality of inclined plates to rotate. At this time, the inclined plates will The gas flowing inside the spiral tube is extracted and blown into the inside of the airbag. When the gaseous liquid nitrogen enters the airbag, it will be affected by the temperature of the liquid nitrogen inside the main body to form cold gas. After that, a part of the gas will flow back to the inside of the swing disk through another airbag and the hollow telescopic column under the blowing of the inclined plate, thereby realizing the reuse of the gaseous liquid nitrogen. The frequency of replenishment of liquid nitrogen can be reduced to a certain extent, and the consumption of liquid nitrogen can be reduced. In addition, the circulating gaseous liquid nitrogen can transfer the cold to the entire storage space, reduce the temperature gradient, reduce the impact of the large temperature difference on the physiological state and activity of the biological sample, and improve the quality and reliability of sample preservation.

[0015] 3. According to the present invention, when the bidirectional threaded rod rotates, the rotating long column will be driven to rotate, when the rotating long column rotates, the hollow plate will be driven to rotate, when the hollow plate rotates, a plurality of L-shaped hollow columns will be driven to rotate, and when the L-shaped hollow column rotates, the hollow circle will be driven to rotate, and at this time, another part of the gaseous liquid nitrogen will flow to the inside of the L-shaped hollow column through the air inlet and the hollow plate, and when the hollow plate rotates, the gaseous liquid nitrogen will flow to the inside of the hollow circle and flow out from the exhaust hole provided on the outer surface of the hollow circle, and at the same time, the hollow circle will shake inside the liquid nitrogen liquid when it shakes and form bubbles inside it, so that the bubbles will drive the surrounding liquid nitrogen to move together with the gas when they rise, thereby accelerating the generation of liquid nitrogen gas, allowing more cold gas to rise and cool the placed samples more fully and continuously, thereby increasing the speed of cold gas generation, thereby further enhancing the cooling storage of the samples and improving the storage efficiency.

[0016] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0018] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the overall cross-sectional structure of the present invention; Figure 3 It is a schematic diagram of the main body of the present invention; Figure 4 It is a schematic diagram of the auxiliary mechanism structure of the present invention; Figure 5 For the present invention Figure 4 Enlarged view of point A in the middle; Figure 6 It is a schematic diagram of the auxiliary mechanism structure of the present invention; Figure 7 It is a schematic diagram of the structure of the circulation mechanism of the present invention; Figure 8 It is a schematic diagram of the structure of the circulation mechanism of the present invention; Fig. 9 It is a schematic diagram of the cooling mechanism structure of the present invention; Fig.10 It is a schematic diagram of the cooling mechanism structure of the present invention; Fig.11 For the present invention Fig.10 Enlarged view of point B in the middle; Fig.12 It is a schematic diagram of the structure of the rotating mechanism of the present invention; Fig.13 It is a schematic diagram of the auxiliary mechanism structure of the present invention; Fig.14 The figure is a flow chart of the method for using the present invention.

[0019] In the accompanying drawings, the components represented by the reference numerals are listed as follows: In the figure: 1, main body; 101, sealing cover 1; 102, fixed block; 103, placement tank; 104, sealing cover 2; 105, placement rack; 106, circular plate 1; 107, conveying pipe; 2, auxiliary mechanism; 201, motor; 202, short column; 203, hollow cylinder; 204, fixed plate 1; 205, telescopic spring column; 206, swing disc; 207, circular plate 2; 208, fixed long column; 209, swing plate; 210, C-shaped plate; 211, fixed cylinder; 212, fixed short plate; 213, rotating plate; 214, fixed Fixed spring plate; 3. Circulation mechanism; 301. Spiral tube; 302. Hollow block; 303. Hollow tube; 304. Flexible hollow tube; 305. One-way valve; 4. Cooling mechanism; 401. Hollow telescopic column; 402. Air bag; 403. Fixed plate two; 404. Bidirectional threaded rod; 405. Fixed short column; 406. Fixed plate three; 407. Inclined plate; 408. Return spring; 409. Limit plate; 5. Rotation mechanism; 501. Rotating long column; 502. Hollow plate; 503. L-shaped hollow column; 504. Hollow circle; 505. Spring. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] See also Figure 1-Figure 14 As shown, the present invention is a gas phase liquid nitrogen biological storage tank, comprising a main body 1, the main body 1 comprising a placement tank 103 arranged inside the main body 1, a sealing cover 104 is slidably connected to the top of the placement tank 103, a sealing cover 101 is fixedly connected to the outer surface of the placement tank 103, the inner wall of the sealing cover 101 is slidably connected to the outer surface of the main body 1, a fixing block 102 is fixedly connected to the end of the sealing cover 101 away from the main body 1, a plurality of placement racks 105 are slidably connected to the inside of the placement tank 103, a circular plate 106 is fixedly connected to the inside of the main body 1, a delivery pipe 107 is fixedly connected to the outer surface of the main body 1, an end of the delivery pipe 107 close to 106 passes through the inner wall of the main body 1 and extends to the inside, a plurality of arc grooves are opened on the inner wall of the main body 1, and further comprising; The auxiliary mechanism 2 includes a motor 201 fixedly connected to the bottom of the placement tank 103, the end of the motor 201 away from the placement tank 103 is fixedly connected to a rotating column, the outer surface of the rotating column is provided with an arc-shaped ring groove, and a plurality of short columns 202 are slidably connected inside the arc-shaped ring groove, the ends of the plurality of short columns 202 away from the rotating column are fixedly connected to a hollow cylinder 203, the end of the hollow cylinder 203 away from the motor 201 is fixedly connected to a plurality of fixed plates 204, the end of the fixed plate 204 close to the motor 201 is fixedly connected to a telescopic spring column 205, and the end of the fixed plate 204 away from the telescopic spring column 205 is fixedly connected to a fixed long column 208. When the motor 201 is working, it will drive the rotating column to rotate. When the rotating column rotates, it will drive the hollow cylinder 203 to swing up and down through the arc-shaped ring groove and the plurality of short columns 202. When the hollow cylinder 203 swings up and down, it will drive the swinging disc 206 to swing up and down through the plurality of fixed long columns 208.

[0022] A circular barrel is provided at the bottom of the hollow cylinder 203, and the circular barrel is slidably connected to the outer surface of the rotating column. A swinging disc 206 is fixedly connected to the side of the circular barrel away from the hollow cylinder 203, and the top of the swinging disc 206 is fixedly connected to a plurality of fixed long columns 208. A plurality of fixed spring plates 214 are fixedly connected to the side of the swinging disc 206 away from the hollow cylinder 203, and the side wall of the fixed spring plate 214 is fixedly connected to the inner wall of the main body 1. A plurality of spring plates 104 are fixedly connected to the side of the swinging disc 206 away from the hollow cylinder 203, and one end of the spring plate away from the motor 201 is fixedly connected to the inner wall of the main body 1. A circular plate 207 is fixedly connected to the end of the rotating column away from the motor 201, and a plurality of telescopic spring columns 205 are fixedly connected to the swinging plate 209 at one end close to the motor 201. The swinging plate A plurality of fixed cylinders 211 are arranged on the side 209 away from the motor 201, and a C-shaped plate 210 is fixedly connected to the outer surface of the fixed cylinder 211, and a fixed short plate 212 is fixedly connected to the outer surface of the fixed cylinder 211 inside the C-shaped plate 210, and the fixed short plate 212 is rotatably connected to a rotating plate 213 at one end away from the fixed cylinder 211, and a spring is fixedly connected to one end of the fixed cylinder 211 away from the swinging plate 209, and one end of the fixed cylinder 211 away from the fixed short plate 212 is slidably connected to the inside of the arc groove, and one end of the spring away from the fixed cylinder 211 is fixedly connected to the bottom of the arc groove, and when the swinging plate 209 swings up and down, the fixed short plate 212 and the fixed cylinder 211 will be driven to swing up and down through the C-shaped plate 210, and when the fixed short plate 212 swings up and down, the rotating plate 213 will be driven to swing up and down.

[0023] The outer surface of the circular plate 106 is provided with a circulation mechanism 3, which includes a spiral tube 301 fixedly connected to the top of the circular plate 106, the end of the spiral tube 301 away from the circular plate 106 is fixedly connected to a hollow block 302, the bottom of the hollow block 302 is fixedly connected to a hollow tube 303, and the end of the hollow tube 303 away from the hollow block 302 is fixedly connected to a flexible hollow tube 304; The spiral tube 301 is connected to the circular plate 106, and a one-way valve 305 is fixedly connected to the inside of the spiral tube 301. When the swing disk 206 swings up and down, the gaseous liquid nitrogen at the top of the swing disk 206 is pressurized to enter the inside of the spiral tube 301 from the one-way valve 305. After the gaseous liquid nitrogen enters the spiral tube 301, it circulates inside the spiral tube 301 and plays a role in circulating and cooling the organisms stored in the placement tank 103.

[0024] A cooling mechanism 4 is provided on the outer surface of the swinging disc 206. The cooling mechanism 4 includes two hollow telescopic columns 401 rotatably connected to the inside of the swinging disc 206. One of the hollow telescopic columns 401 is connected to the flexible hollow tube 304. A square sliding groove is provided on the outer surface of one of the hollow telescopic columns 401. An end of the hollow telescopic column 401 away from the swinging disc 206 is fixedly connected to an airbag 402. The two airbags 402 are connected. When the gaseous liquid nitrogen enters the flexible hollow tube 304, it will flow to the inside of the hollow telescopic column 401. When the swinging disc 206 swings up and down, the two airbags 402 will also swing up and down together.

[0025] The inner wall of one of the hollow telescopic columns 401 is fixedly connected with a fixing plate 2 403, and the end of the fixing plate 2 403 close to the airbag 402 is rotatably connected with a bidirectional threaded rod 404, and the outer surface of the bidirectional threaded rod 404 is rotatably connected with a fixing short column 405, and the end of the fixing short column 405 away from the bidirectional threaded rod 404 is fixedly connected with a fixing plate 3 406, and the end of the fixing plate 3 406 away from the fixing short column 405 passes through the square sliding groove and extends to the outside, and the end of the fixing plate 3 406 away from the fixing plate 2 403 is fixedly connected to the outer surface of the hollow telescopic column 401. When the hollow telescopic column 401 contracts, the fixing plate 3 406 and the fixing short column 405 will slide on the outer surface of the bidirectional threaded rod 404. Since the fixing plate 3 406 is fixedly connected to the hollow telescopic column 401, the bidirectional threaded rod 404 will rotate at this time.

[0026] The outer surface of the bidirectional threaded rod 404 is fixedly connected with a plurality of inclined plates 407, and a return spring 408 is arranged on the outer surface of the bidirectional threaded rod 404. The end of the return spring 408 away from the inclined plate 407 is fixedly connected with a limit plate 409, and the limit plate 409 is fixedly connected to the inner wall of the hollow telescopic column 401. When the gaseous liquid nitrogen enters the airbag 402, it will be affected by the temperature of the liquid nitrogen inside the main body 1 to form cold gas, and then a part of the gas will flow back to the inside of the swing disc 206 through another airbag 402 and the hollow telescopic column 401 under the blowing of the inclined plate 407.

[0027] A rotating mechanism 5 is provided on the outer surface of the bidirectional threaded rod 404, and the rotating mechanism 5 includes a rotating long column 501 fixedly connected to the bottom of the bidirectional threaded rod 404, and the end of the rotating long column 501 away from the bidirectional threaded rod 404 passes through the inner wall of the hollow plate 502 and extends to the inside, and the interior of the rotating long column 501 is hollow, and two air inlet holes are provided on the outer surface of the rotating long column 501, and the end of the rotating long column 501 away from the limiting plate 409 is fixedly connected to the hollow plate 502, and a plurality of L-shaped hollow columns 503 are fixedly connected to the outer surface of the hollow plate 502. When the bidirectional threaded rod 404 rotates, the rotating long column 501 will be driven to rotate, and when the rotating long column 501 rotates, the hollow plate 502 will be driven to rotate.

[0028] The L-shaped hollow column 503 is made of a flexible material. One end of the L-shaped hollow column 503 away from the hollow plate 502 is fixedly connected to a hollow circle 504. A plurality of exhaust holes are provided on the outer surface of the hollow circle 504. A plurality of springs 505 are fixedly connected to the outer surface of the hollow plate 502. One end of the spring 505 away from the hollow plate 502 is fixedly connected to the L-shaped hollow column 503. When the hollow plate 502 rotates, gaseous liquid nitrogen will flow into the hollow circle 504 and out from the exhaust holes provided on the outer surface of the hollow circle 504. At the same time, the hollow circle 504 will shake inside the liquid nitrogen when it shakes and form bubbles inside it.

[0029] A method for using a gas phase liquid nitrogen biological storage tank, the gas phase liquid nitrogen biological storage tank, the method comprising the following steps: S1: Open the sealing cover 104; S2: Place the sample; S3: transport liquid nitrogen; S4: Cooling storage.

[0030] When in use, first use the delivery pipe 107 to deliver liquid nitrogen to the inside of the main body 1, and then start the motor 201. When the motor 201 is working, it will drive the rotating column to rotate. When the rotating column rotates, it will drive the hollow cylinder 203 to swing up and down through the arc-shaped ring groove and a plurality of short columns 202. When the hollow cylinder 203 swings up and down, it will drive the swing disc 206 to swing up and down through a plurality of fixed long columns 208. When the swing disc 206 swings up and down, the gaseous liquid nitrogen at the bottom of the main body 1 will flow to the top of the swing disc 206 through the circular barrel. When the hollow cylinder 203 swings up and down, it will drive the swing plate 209 to swing up and down through a plurality of telescopic spring columns 205. When the swing plate 209 swings up and down, it will drive the fixed short plate 212 and the fixed cylinder 2 through the C-shaped plate 210. 11 swings up and down, when the fixed short plate 212 swings up and down, it drives the rotating plate 213 to swing up and down, when the fixed cylinder 211 swings up and down, it swings left and right under the action of the arc groove, when the swinging disc 206 swings up and down, the gas phase liquid nitrogen located on the top of the swinging disc 206 is under pressure and enters the inside of the spiral tube 301 from the one-way valve 305, when the gas phase liquid nitrogen enters the spiral tube 301, it circulates inside the spiral tube 301 and plays a role in circulating and cooling the biological stored in the placement tank 103, and by making the gas phase liquid nitrogen flow in the spiral tube 301, the material placed in the spiral tube 301 can be evenly cooled, which can better achieve uniform temperature distribution, and thus can improve the storage quality of the sample during storage.

[0031] When the gas phase liquid nitrogen enters the spiral tube 301, it flows into the flexible hollow tube 304 through the hollow block 302 and the hollow tube 303. When the gas phase liquid nitrogen enters the flexible hollow tube 304, it will flow into the hollow telescopic column 401. When the swinging disc 206 swings up and down, the two air bags 402 will also swing up and down together. When the swinging disc 206 moves downward, since the air bags 402 float on the surface of the liquid nitrogen, they will push the hollow telescopic column 401 to contract. When the hollow telescopic column 401 contracts, the fixed plate three 406 and the fixed short column 405 will slide on the outer surface of the two-way threaded rod 404. Since the fixed plate three 406 is fixedly connected to the hollow telescopic column 401, the two-way threaded rod 404 will rotate at this time. When the two-way threaded rod 404 rotates, it will drive several The inclined plate 407 rotates, and the inclined plate 407 extracts the gas flowing inside the spiral tube 301 and blows it into the air bag 402. After the gaseous liquid nitrogen enters the air bag 402, it will be affected by the temperature of the liquid nitrogen in the main body 1 to form cold gas. After that, part of the gas will flow back to the inside of the swing disc 206 through another air bag 402 and the hollow telescopic column 401 under the blowing of the inclined plate 407, so as to realize the reuse of the gaseous liquid nitrogen, reduce the replenishment frequency of liquid nitrogen to a certain extent, reduce the consumption of liquid nitrogen, and the circulating gaseous liquid nitrogen can transfer the cold to the entire storage space, reduce the temperature gradient, reduce the influence of the large temperature difference on the physiological state and activity of the biological sample, and improve the quality and reliability of sample preservation.

[0032] When the bidirectional threaded rod 404 rotates, it will drive the rotating long column 501 to rotate, and when the rotating long column 501 rotates, it will drive the hollow plate 502 to rotate, and when the hollow plate 502 rotates, it will drive several L-shaped hollow columns 503 to rotate, and when the L-shaped hollow column 503 rotates, it will drive the hollow circle 504 to rotate, and at this time, another part of the gaseous liquid nitrogen flows into the L-shaped hollow column 503 through the air inlet and the hollow plate 502, and when the hollow plate 502 rotates, the gaseous liquid nitrogen will flow into the hollow circle 504 and flow out from the exhaust hole opened on the outer surface of the hollow circle 504, and at the same time, the hollow circle 504 will shake inside the liquid nitrogen liquid when it shakes and form bubbles inside it, so that the bubbles will drive the surrounding liquid nitrogen and gas to move together when they rise, thereby accelerating the generation of liquid nitrogen gas, allowing more cold gas to rise and cool the placed samples more fully and continuously, increasing the speed of cold gas generation, thereby further enhancing the cooling storage of samples and improving storage efficiency.

[0033] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A gas phase liquid nitrogen biological storage tank, comprising a main body (1), the main body (1) comprising a placement tank (103) arranged inside the main body (1), the top of the placement tank (103) being slidably connected to a sealing cover 2 (104), the outer surface of the placement tank (103) being fixedly connected to a sealing cover 1 (101), the inner wall of the sealing cover 1 (101) being slidably connected to the outer surface of the main body (1), the end of the sealing cover 1 (101) away from the main body (1) being fixedly connected to a fixing block (102), the interior of the placement tank (103) being slidably connected to a plurality of placement racks (105), the interior of the main body (1) being fixedly connected to a circular plate 1 (106), the outer surface of the main body (1) being fixedly connected to a delivery pipe (107), the end of the delivery pipe (107) close to 106 penetrating the inner wall of the main body (1) and extending to the interior, the inner wall of the main body (1) being provided with a plurality of arc grooves, characterized in that: Also includes: The auxiliary mechanism (2) comprises a motor (201) fixedly connected to the bottom of the placement tank (103); one end of the motor (201) away from the placement tank (103) is fixedly connected to a rotating column; an arc-shaped annular groove is formed on the outer surface of the rotating column; a plurality of short columns (202) are slidably connected inside the arc-shaped annular groove; one end of the plurality of short columns (202) away from the rotating column is fixedly connected to a hollow cylinder (203); one end of the hollow cylinder (203) away from the motor (201) is fixedly connected to a plurality of fixed plates (204); one end of the fixed plate (204) close to the motor (201) is fixedly connected to a telescopic spring column (205); and one end of the fixed plate (204) away from the telescopic spring column (205) is fixedly connected to a fixed long column (208).

2. A gas phase liquid nitrogen biological storage tank according to claim 1, characterized in that: A circular barrel is provided at the bottom of the hollow cylinder (203), and the circular barrel is slidably connected to the outer surface of the rotating column. A swinging disc (206) is fixedly connected to the side of the circular barrel away from the hollow cylinder (203). The top of the swinging disc (206) is fixedly connected to a plurality of fixed long columns (208). A plurality of fixed spring plates (214) are fixedly connected to the side of the swinging disc (206) away from the hollow cylinder (203). The side wall of the fixed spring plate (214) is fixedly connected to the inner wall of the main body (1). One end of the spring plate away from the motor (201) is fixedly connected to the inner wall of the main body (1). One end of the rotating column away from the motor (201) is fixedly connected to a second circular plate (207). The plurality of telescopic spring columns (205) are close to the motor (201). ) is fixedly connected to a swing plate (209) at one end, a plurality of fixed cylinders (211) are provided on a side of the swing plate (209) away from the motor (201), a C-shaped plate (210) is fixedly connected to the outer surface of the fixed cylinder (211), a fixed short plate (212) is fixedly connected to the outer surface of the fixed cylinder (211) located inside the C-shaped plate (210), an end of the fixed short plate (212) away from the fixed cylinder (211) is rotatably connected to a rotating plate (213), an end of the fixed cylinder (211) away from the swing plate (209) is fixedly connected to a spring, an end of the fixed cylinder (211) away from the fixed short plate (212) is slidably connected to the inside of the arc groove, and an end of the spring away from the fixed cylinder (211) is fixedly connected to the bottom of the arc groove.

3. A gas phase liquid nitrogen biological storage tank according to claim 2, characterized in that: A circulation mechanism (3) is provided on the outer surface of the circular plate one (106), the circulation mechanism (3) comprising a spiral tube (301) fixedly connected to the top of the circular plate one (106), the end of the spiral tube (301) away from the circular plate one (106) being fixedly connected to a hollow block (302), the bottom of the hollow block (302) being fixedly connected to a hollow tube (303), and the end of the hollow tube (303) away from the hollow block (302) being fixedly connected to a flexible hollow tube (304); The spiral tube (301) is connected to the circular plate (106), and a one-way valve (305) is fixedly connected to the interior of the spiral tube (301).

4. A gas phase liquid nitrogen biological storage tank according to claim 3, characterized in that: A cooling mechanism (4) is provided on the outer surface of the swinging disc (206), and the cooling mechanism (4) comprises two hollow telescopic columns (401) rotatably connected to the inside of the swinging disc (206), one of the hollow telescopic columns (401) being connected to the flexible hollow tube (304), a square sliding groove being provided on the outer surface of one of the hollow telescopic columns (401), and an air bag (402) being fixedly connected to one end of the hollow telescopic column (401) away from the swinging disc (206), and the two air bags (402) being connected to each other.

5. A gas phase liquid nitrogen biological storage tank according to claim 4, characterized in that: The inner wall of one of the hollow telescopic columns (401) is fixedly connected to a second fixing plate (403); an end of the second fixing plate (403) close to the airbag (402) is rotatably connected to a bidirectional threaded rod (404); an outer surface of the bidirectional threaded rod (404) is rotatably connected to a short fixing column (405); an end of the short fixing column (405) away from the bidirectional threaded rod (404) is fixedly connected to a third fixing plate (406); an end of the third fixing plate (406) away from the short fixing column (405) passes through a square sliding groove and extends to the outside; an end of the third fixing plate (406) away from the second fixing plate (403) is fixedly connected to the outer surface of the hollow telescopic column (401).

6. A gas phase liquid nitrogen biological storage tank according to claim 5, characterized in that: A plurality of inclined plates (407) are fixedly connected to the outer surface of the bidirectional threaded rod (404), a return spring (408) is provided on the outer surface of the bidirectional threaded rod (404), one end of the return spring (408) away from the inclined plate (407) is fixedly connected to a limit plate (409), and the limit plate (409) is fixedly connected to the inner wall of the hollow telescopic column (401).

7. A gas phase liquid nitrogen biological storage tank according to claim 6, characterized in that: The outer surface of the bidirectional threaded rod (404) is provided with a rotating mechanism (5), and the rotating mechanism (5) comprises a rotating long column (501) fixedly connected to the bottom of the bidirectional threaded rod (404), and one end of the rotating long column (501) away from the bidirectional threaded rod (404) penetrates the inner wall of the hollow plate (502) and extends to the inside, and the inside of the rotating long column (501) is hollow, and two air inlet holes are provided on the outer surface of the rotating long column (501), and one end of the rotating long column (501) away from the limiting plate (409) is fixedly connected to the hollow plate (502), and a plurality of L-shaped hollow columns (503) are fixedly connected to the outer surface of the hollow plate (502).

8. The gas phase liquid nitrogen biological storage tank according to claim 7, characterized in that: The L-shaped hollow column (503) is made of a flexible material; one end of the L-shaped hollow column (503) away from the hollow plate (502) is fixedly connected to a hollow circle (504); a plurality of exhaust holes are provided on the outer surface of the hollow circle (504); a plurality of springs (505) are fixedly connected to the outer surface of the hollow plate (502); and one end of the spring (505) away from the hollow plate (502) is fixedly connected to the L-shaped hollow column (503).

9. A method for using a gas phase liquid nitrogen biological storage tank, characterized in that: Using a gas phase liquid nitrogen biological storage tank as claimed in claim 8, the method comprises the following steps: S1: Open the sealing cover 1 (104); S2: Place the sample; S3: transport liquid nitrogen; S4: Cooling storage.

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