A weed control composition for preventing and controlling ophiopogon sphaerocephala

Abstract

The invention relates to the technical field of pesticides, and specifically discloses a herbicidal composition for preventing and controlling Phragmites australis in wheat fields. The herbicidal composition is composed of isoproturon, palmitic acid, ferulic acid and an adjuvant, and the mass percentage of each component is: 25.00-35.00% of isoproturon, 2.50-12.50% of palmitic acid, 2.50-12.50% of ferulic acid, 2.50-7.00% of an adjuvant, and the rest is deionized water. The herbicidal composition containing isoproturon, palmitic acid and ferulic acid of the invention is sprayed uniformly on the plant surface at 100g per mu diluted with 30kg of water at one time during the three-leaf stage to the tillering stage before wheat overwintering or the jointing stage to the booting stage after overwintering, and the biomass control effect of Phragmites australis after the application of the medicine exceeds 80.00%, and it is safe for wheat and significantly increases the yield of wheat.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and specifically discloses a weed control composition for preventing and controlling ophiae scabra. Background Art

[0002] Wheat is one of the main food crops. Phragmites australis is a malignant invasive weed in wheat fields. It often causes a yield reduction of more than 30% for wheat crops, or even a total crop failure in severe cases, resulting in huge economic losses. How to safely and effectively control Phragmites australis in wheat fields has attracted widespread attention.

[0003] Chemical control is the main measure to control Phalaenopsis spp. in wheat fields at home and abroad, but there are few types of herbicides to control Phalaenopsis spp. in wheat fields, which is easy to induce resistance. In order to control the occurrence and harm of Phalaenopsis spp. in wheat fields, scholars at home and abroad have widely carried out research on physical control, chemical control, biological control and other control technologies for many years. However, due to the similar phenological period of Phalaenopsis spp. and wheat, and similar morphological characteristics in the seedling stage, it is difficult to distinguish them in the field, and it is difficult to control them through physical control methods such as manual pulling or mechanical cutting. Although biological control has been a hot topic of research at home and abroad in recent years, there has been no report on its application in wheat fields; chemical control saves labor and time, and has always been one of the main means of controlling Phalaenopsis spp. in wheat fields at home and abroad. However, Phalaenopsis spp. and wheat crops are both grass plants, resulting in a single variety of chemical herbicides for controlling Phalaenopsis spp. in wheat fields. Currently, only a few herbicide varieties such as Isoproturon, Pinoxaden, and Fenoxaprop-p-ethyl can be used to control Phalaenopsis in wheat fields. Since the herbicide varieties for controlling Phalaenopsis are relatively single, long-term and large-scale application can easily lead to the development of drug resistance, making some chemical herbicides gradually unusable for controlling Phalaenopsis in wheat fields. This also makes it increasingly difficult to change the chemical control of Phalaenopsis in wheat fields.

[0004] Isoproturon, 4-amino-3,5-dichloro-6-fluoro-pyridine-2-oxyacetic acid, is a systemic selective herbicide of the pyridyloxyacetic acid class. It was also one of the main chemical herbicides used to control Phalaenopsis spp. in wheat fields at home and abroad from the 1970s to the 1990s. However, the long-term and large-scale use of isoproturon has also led to the gradual increase of Phalaenopsis spp.'s resistance to it. In order to control Phalaenopsis spp. in wheat fields, people have continuously increased the field dosage of isoproturon. However, with the increase in the field dosage of isoproturon, it not only causes pesticide damage to wheat crops, but also affects the growth of the next crop. At the same time, long-term and large-scale application also pollutes the soil, water and other ecological environments. Because Phalaenopsis spp. has developed a high resistance to isoproturon, it is currently difficult to use isoproturon to control Phalaenopsis spp. in wheat fields at home and abroad.

[0005] Palmitic acid, also known as hexadecanoic acid, is a saturated higher fatty acid and one of the main components of many plant secondary metabolites. As palmitic acid is a plant secondary metabolite, it has low toxicity to humans and animals and is safe for the environment, making it an important industrial raw material. Studies have shown that palmitic acid has moisturizing, anti-oxidation, cleansing, and auxiliary inhibition of keratinocyte formation. It is widely used in industries such as candles, soaps, greases, softeners, and synthetic detergents, and is an industrial raw material with high economic value.

[0006] Ferulic acid, 3-methoxy-4-hydroxycinnamic acid, is one of the main components of many plant secondary metabolites. Since ferulic acid is a plant secondary metabolite, it has low toxicity to humans and animals and is safe for the environment, making it an important industrial raw material. Studies have shown that ferulic acid has anti-radiation, antioxidant, antibacterial and antiviral effects. In recent years, studies have also found that ferulic acid has inhibitory activity against some weeds.

[0007] At present, there is no report on combining palmitic acid and ferulic acid to enhance the effectiveness of isoproturon in controlling Phalaenopsis sylvestris in wheat fields at home and abroad. Summary of the invention

[0008] Aiming at the shortcomings of isoproturon in preventing and controlling phragmites in wheat fields, such as low efficacy, poor safety to wheat, and low control effect on older phragmites, the present invention provides a herbicidal composition for preventing and controlling phragmites in wheat fields, containing isoproturon, palmitic acid and ferulic acid, and a preparation method and use thereof. According to studies such as indoor bioassay, field efficacy test and safety evaluation, the herbicidal composition of the present invention has the advantages of high efficacy, low dosage of chemical herbicides, high control effect on older phragmites, and safety and harmlessness to wheat when preventing and controlling phragmites in wheat fields.

[0009] The scheme of the present invention is:

[0010] A herbicidal composition for controlling Phalaenopsis sphaerocephala, characterized in that it comprises the following raw materials in percentage by mass:

[0011] Isoproturon 25.00~35.00%;

[0012] Palmitic acid 2.50-12.50%;

[0013] Ferulic acid 2.50-12.50%;

[0014] Additives 2.50~7.00%;

[0015] Deionized water to 100.00%.

[0016] As a preferred technical solution, the auxiliary agent consists of ethoxy-modified trisiloxane, dodecyl diphenyl ether disulfonate and epoxy soybean oil.

[0017] As a preferred technical solution, the auxiliary agent includes the following raw materials in percentage by mass:

[0018] Ethoxy modified trisiloxane 1.00~3.00%;

[0019] Dodecyl diphenyl ether disulfonate 1.00~3.00%;

[0020] Epoxidized soybean oil 0.50~1.00%.

[0021] The present invention also discloses a method for preparing a herbicidal composition for controlling Phalaenopsis sphaerocephala, comprising the following steps:

[0022] S1, add chloroisopropuron to the reaction tank a according to the ratio, stir evenly, inject the composition into the multi-stage grinder for grinding, and discharge the ground material after 98% of the fineness passes 2-4 um, and the material a is set aside;

[0023] S2, adding palmitic acid, ferulic acid, ethoxy-modified trisiloxane, dodecyl diphenyl ether disulfonate and epoxy soybean oil into reaction tank b according to the proportion, and stirring until completely mixed;

[0024] S3, injecting material a and the material in reaction tank b into an emulsification tank, adding deionized water according to the proportion, and then high-speed shearing and emulsification to obtain a herbicidal composition for controlling Phalaenopsis calyx.

[0025] The present invention also discloses a use of a weed control combination for preventing and controlling Phalaenopsis sphaerocephala in wheat fields, which is used for preventing and controlling Phalaenopsis sphaerocephala in wheat fields. Specifically:

[0026] During the three-leaf stage to tillering stage and the jointing stage to booting stage of wheat, the herbicidal composition of the present invention is evenly sprayed on the plant surface at one time at a rate of 100 g per mu diluted with 30 kg of water.

[0027] Advantages of the present invention:

[0028] (1) Compared with the single chemical herbicide isoproturon, the herbicidal composition of the present invention has a higher efficacy against Pseudostellaria scabra.

[0029] Indoor potted plant experiments have shown that the control effect of single chemical herbicide isoproturon on Phragmites australis in wheat fields at 25.00, 30.00 and 35.00 g ai / mu was 25.19%, 27.12% and 29.87% respectively 30 days after application. The herbicidal composition containing isoproturon, palmitic acid and ferulic acid of the present invention has a control effect on Phragmites australis higher than 70.00%, and the control effect is significantly improved.

[0030] Field efficacy tests show that when the chemical herbicide isoproturon is used at a dosage of 100 g ai per mu, the plant control efficacy and biomass control efficacy against Phragmites australis are 52.17%, 51.59%, 59.51%, and 56.49%, respectively, during the wheat greening stage to jointing stage and jointing stage to booting stage. When the herbicide composition of the present invention is used at a dosage of 25.00 g ai per mu, the control efficacy against Phragmites australis is significantly higher than 60.00% during the wheat three-leaf stage to tillering stage and jointing stage to booting stage, and is significantly higher than the control efficacy of the chemical herbicide isoproturon alone against Phragmites australis.

[0031] (2) Compared with single chemical herbicide isoproturon, the herbicidal composition of the present invention significantly reduces the amount of chemical herbicide isoproturon used in the field.

[0032] Field efficacy tests show that when the chemical herbicide isoproturon is used at a dosage of 100 g ai per mu, its plant control efficiency and biomass control efficiency against Phalaenopsis spp. are 52.17%, 51.59% and 59.51%, 56.49% respectively. When the herbicidal composition of the present invention is used at a dosage of 25.00 g ai per mu, the plant control efficiency against Phalaenopsis spp. is higher than 70% and the biomass control efficiency is higher than 80% during the three-leaf stage to tillering stage of wheat; the plant control efficiency against Phalaenopsis spp. is higher than 60% and the biomass control efficiency is higher than 80% during the jointing stage to the booting stage. As the mass percentage of isoproturon in the inventive composition increases, the control efficiency of the inventive herbicidal composition against Phalaenopsis spp. continues to improve. The research results also show that the herbicidal composition of the present invention has a high efficacy against Pseudostellaria scabra and can reduce the field dosage of the chemical herbicide isoproturon by more than 75.00%.

[0033] (3) The herbicidal combination of the present invention is significantly safer to wheat than isoproturon alone.

[0034] The field safety evaluation test showed that the herbicidal composition of the present invention was applied during the three-leaf stage to the tillering stage and the jointing stage to the booting stage of wheat, and no abnormality or phytotoxicity was observed in the wheat. However, at a dosage of 100.00 g ai / mu of the chemical herbicide isoproturon, the wheat in the treatment group was completely killed. Therefore, the herbicidal composition of the present invention is safer for wheat than the single chemical herbicide isoproturon.

[0035] (3) The herbicidal composition of the present invention can be used for the prevention and control of Phalaenopsis spp. in mid- and late-stage wheat fields, and is safe and efficient.

[0036] In the jointing stage to the booting stage of wheat, the chemical herbicide isoproturon has a plant control effect and fresh weight control effect on Phalaenopsis sibiricum at 100 g ai / mu, respectively. However, at this dosage, the wheat is completely dead. The herbicidal composition of the present invention has a biomass control effect of more than 80% on Phalaenopsis sibiricum at 25.00-35.00% isoproturon, 2.50-12.50% palmitic acid and 2.50-12.50% ferulic acid, which is not only significantly higher than the control effect of a single dose of isoproturon (100 g ai / mu), but also safe and non-toxic to wheat. Therefore, the herbicidal composition of the present invention can be used for the prevention and control of older Phalaenopsis sibiricum in wheat fields in the middle and late stages, and is safe and efficient. DETAILED DESCRIPTION

[0037] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments.

[0038] Example 1: Effects of different organic acids and their combinations on the control of Phalaenopsis dasyphylla by isoproturon

[0039] 1.1 Test materials

[0040] The effects of different organic acids and their combinations on the control of Phalaenopsis spp. by isoproturon were tested in indoor potted plants. The different organic acids and their combinations were: vanillic acid (C8H8O4, ≥98.00%), fulvic acid (C 14 H 12 O8, ≥95.00%), p-hydroxybenzoic acid (C7H6O3, ≥99.50%), ferulic acid (C 10 H 10 O4, ≥99.00%), linoleic acid (C 18 H 32 O2, ≥99.00%), palmitic acid (C 16 H 32 O2, ≥99.00%), palmitic acid (C 16 H 32 O2, ≥99.00%) + ferulic acid (C 10 H 10 O4, ≥99.00%), the above organic acids were purchased from MacLean Chemical Reagent (China) Co., Ltd. 50% Isoproturon suspension (SC), Suzhou Bianjing Plant Protection Technology Co., Ltd.

[0041] 1.2 Test methods

[0042] The biological activities of chemical herbicide isoproturon and six organic acids (vanillic acid, fulvic acid, p-hydroxybenzoic acid, ferulic acid, linoleic acid, palmitic acid) and their combination (palmitic acid + ferulic acid) against Phalaenopsis scabra were determined by post-emergence stem-leaf spraying under greenhouse conditions.

[0043] The specific method is: in an area of ​​0.02 m 2 3 kg of dried and crushed soil was placed in a plastic pot of 20 cm×10 cm×9.5 cm (length×width×height), and the soil in each plastic pot was completely moistened. The seeds of Phalaenopsis dasyphylla collected that year, with full seeds and out of dormancy (germination rate ≥95.00%) were selected, and 100 seeds were sown in each pot. After sowing, they were covered with 1-5 mm of fine soil and then cultured in a greenhouse (temperature of 20℃ / 10℃, about 12h light / 12h dark). After 30 days, the pesticide was applied in the spray tower according to the designed dosage of the experiment by spraying stems and leaves (spraying volume of 30 L / mu, spraying pressure of 0.6 kpa), and the control was sprayed with pure water. Each treatment was repeated 4 times (4 pots). After 2 hours of application, the plants were placed in the greenhouse for cultivation, and the culture conditions were the same as in the previous period.

[0044] 15 days after application, all the Phalaenopsis scabra in the pots were taken out, the dead branches and leaves above the ground and the dead and rotten rhizomes underground were removed, the biomass was weighed after washing and drying, and compared with the control, and the biological activity was calculated.

[0045] 1.3 Calculation method

[0046] The Gowing method was used to evaluate the combined effect of two mixed drugs, and the formula is as follows:

[0047] Actual biomass inhibition rate (%) = [(control weed biomass - treatment weed biomass) / control weed biomass] × 100

[0048] Theoretical inhibition rate E0 (%) = (X + Y) - (XY / 100)

[0049] In the formula, X is the weed control effect of treatment group A relative to the control group when the dosage of herbicide A is p; Y is the weed control effect of treatment group B relative to the control group when the dosage of herbicide B is q; E0 is the theoretical control effect when the dosage of herbicide A is p + the dosage of herbicide B is q. E is the actual control effect when the dosage of herbicide A is p + the dosage of herbicide B is q.

[0050] The actual inhibition rate (E) was compared with the theoretical inhibition rate (E0). When the E-E0 value was >10%, it was a synergistic effect, when the E-E0 value was <-10%, it was an antagonistic effect, and when the E-E0 value was between -10% and 10%, it was an additive effect. All data were statistically analyzed using DPS v9.01 software, and the significance of the differences among the treatments was tested using a randomized block design combined with Duncan's new multiple range method.

[0051] Table 1 Analysis of the biomass control effects of isoproturon, organic acids and their combination on Phalaenopsis sphaerocephala and their combined effects

[0052]

[0053] Note: Lowercase letters in the table indicate the significant difference of the data in the same column at the 5% level. The same letters indicate insignificant difference, while different letters indicate significant difference.

[0054] As shown in Table 1, when the six organic acids were at 5.00 g ai / mu, palmitic acid had the highest biomass control effect on Phalaenopsis sibiricum (12.29%), and fulvic acid had the lowest control effect on Phalaenopsis sibiricum (-6.32%), indicating that the six organic acids had low control effects on Phalaenopsis sibiricum, among which fulvic acid promoted the growth of Phalaenopsis sibiricum. There were significant differences in the biomass control effects of the combination of the six organic acids and isoproturon on Phalaenopsis sibiricum. When the mass percentage of isoproturon and organic acid was 50.00 + 5.00 g ai / mu, the combination of isoproturon and palmitic acid had the highest measured biomass control effect on Phalaenopsis sibiricum (57.06%), and the combined action index of the two was 13.98, but there was no significant difference with the control effect of ferulic acid and isoproturon on Phalaenopsis sibiricum (53.11%). In addition, the study also found that when isoproturon, palmitic acid and ferulic acid were added at a mass percentage of 50.00+5.00+5.00 g ai / mu, the control effect on Phragmites australis was 80.07%, which was significantly higher than that of isoproturon alone, six organic acids alone, and a mixture of isoproturon and organic acids. The above research results show that the combination of palmitic acid and ferulic acid can significantly improve the control effect of the chemical herbicide isoproturon on Phragmites australis, and is significantly better than other single organic acids.

[0055] Example 2: Effects of different mass percentages of palmitic acid and ferulic acid combinations on the control of Phalaenopsis spp. by isoproturon

[0056] 2.1 Test materials

[0057] The isoproturon, palmitic acid and ferulic acid tested were the same as in Example 1.

[0058] 2.2 Test methods

[0059] Based on the results of Example 1, a potted experiment was conducted under greenhouse conditions to study the biological activity of different mass percentages of palmitic acid and ferulic acid compositions on isoproturon to control Phragmites australis. First, Phragmites australis was cultivated under greenhouse conditions, and the specific cultivation method was the same as in Example 1. After 30 days, the agent was applied in a spray tower according to the designed dosage using a stem and leaf spray method (spraying amount was 30.00 L / mu, spray pressure was 0.6 kpa), and the blank was sprayed with distilled water (see specific implementation), and each treatment was repeated 4 times (4 pots). After application, each test treatment continued to be cultured in the greenhouse, and the culture conditions were the same as in the previous period. 15 days after the drug, each pot of Phragmites australis was completely dug out, and the surviving plants were counted. The dead stems and leaves above ground and the dead roots below ground of the surviving plants were removed and cleaned, and the fresh weight was weighed after the water was absorbed with absorbent paper.

[0060] 2.3 Calculation method:

[0061] The Gowing method was used to evaluate the combined effect of two mixed drugs, and the formula is as follows:

[0062] Actual biomass inhibition rate (%) = [(control weed biomass - treatment weed biomass) / control weed biomass] × 100

[0063] Theoretical inhibition rate E0 (%) = (X + Y) - (XY / 100)

[0064] In the formula, X is the weed control effect of treatment group A relative to the control group when the dosage of herbicide A is p; Y is the weed control effect of treatment group B relative to the control group when the dosage of herbicide B is q; E0 is the theoretical control effect when the dosage of herbicide A is p + the dosage of herbicide B is q. E is the actual control effect when the dosage of herbicide A is p + the dosage of herbicide B is q.

[0065] The actual inhibition rate (E) was compared with the theoretical inhibition rate (E0). When the E-E0 value was >10%, it was a synergistic effect, when the E-E0 value was <-10%, it was an antagonistic effect, and when the E-E0 value was between -10% and 10%, it was an additive effect. All data were statistically analyzed using DPS v9.01 software, and the significance of the differences among the treatments was tested using a randomized block design combined with Duncan's new multiple range method.

[0066] Table 2 Effects of palmitic acid and ferulic acid combination on the efficacy of isoproturon in controlling Phalaenopsis spp.

[0067]

[0068] Note: Lowercase letters in the table indicate significant comparison of data in the same column at the 5% level. The same letters indicate insignificant differences, otherwise significant differences.

[0069] It can be seen from Table 2 that when the herbicidal composition contains 20.00 g ai / mu of isoproturon, 2.50-12.50 ga.i. / mu of palmitic acid, and 2.50-12.50 g ai / mu of ferulic acid, the control effect on Phalaenopsis spp. is higher than 50%; and when isoproturon is 25.00 g ai / mu, the control effect on Phalaenopsis spp. is higher than 75.00%. Later, as the content of isoproturon in the composition increases, the control effect on Phalaenopsis spp. also increases, but the control effect of the combination of isoproturon, palmitic acid and ferulic acid on Phalaenopsis spp. is significantly higher than that of the single agent of isoproturon and the combination of palmitic acid and ferulic acid. In addition, the study also found that when the content of isoproturon in the composition was 35.00 g ai / mu and 40.00 g ai / mu, the control effect of the composition on Phalaenopsis spp. was not significantly different, so 40.00 g ai / mu of isoproturon in the herbicidal composition did not meet the principles of environmental protection and resource conservation. In summary, when the content of isoproturon was 25.00-35.00 g ai / mu, the content of palmitic acid was 2.50-12.50 g ai / mu, and the content of ferulic acid was 2.50-12.50 g ai / mu, the herbicidal composition had the highest control effect on Phalaenopsis spp.

[0070] Example 3: Preparation of a herbicidal composition containing isoproturon, palmitic acid and ferulic acid

[0071] The proportions of the herbicidal composition are calculated by mass percentage: 25.00-35.00% of isoproturon, 2.50-12.50% of palmitic acid, 2.50-12.50% of ferulic acid, 1.00-3.00% of ethoxy-modified trisiloxane, 1.00-3.00% of dodecyl diphenyl ether disulfonate, and 0.5-1.00% of epoxidized soybean oil.

[0072] The preparation method of the herbicidal composition suspension is as follows:

[0073] S1, add chloroisopropuron to the reaction tank a according to the ratio, stir evenly, inject the composition into the multi-stage grinder for grinding, and discharge the ground material after 98% of the fineness passes 2-4 um, and the material a is set aside;

[0074] S2, adding palmitic acid, ferulic acid, ethoxy-modified trisiloxane, dodecyl diphenyl ether disulfonate and epoxy soybean oil into reaction tank b according to the proportion, and stirring until completely mixed;

[0075] S3, injecting material a and the material in reaction tank b into an emulsification tank, adding deionized water according to the proportion, and then high-speed shearing and emulsification to obtain a herbicidal composition for controlling Phalaenopsis calyx.

[0076] Example 4: Evaluation of the control ability of the herbicidal composition of the present invention on Phragmites australis when applied at different times in wheat fields

[0077] 3.1 Test materials

[0078] The isoproturon, palmitic acid and ferulic acid tested were the same as those in Example 2, and the wheat variety tested was Yunmai No. 8, a main variety grown in Yunnan Province.

[0079] 3.2 Test methods

[0080] Based on Example 2, the control efficacy of isoproturon, palmitic acid and a combination thereof on Phalaenopsis tataricus at different application periods was evaluated under field conditions through a randomized block experiment.

[0081] The test site is located in Jiangchang Village, Xiazhuang Township, Xiangyun County, Dali Prefecture, Yunnan Province (100.4617°E, 25.2439°N, 1759.4 m above sea level).

[0082] The soil type is clay loam with clay content >90%; soil pH is 6.3 and organic matter content is 2.914%.

[0083] The fertility is medium, with total nitrogen 0.257 mg / kg, total phosphorus 0.103 mg / kg, and total potassium 1.6 mg / kg.

[0084] The previous crop was rice. After the rice was harvested, the rice fields were plowed. On October 15, 2023, wheat was planted in rows by machine sowing. The management measures after sowing were consistent with local standards, including irrigation, fertilization, and pest and disease control. Conventional management measures were adopted. During the experiment, no other herbicides or plant growth regulators were used except the agents used in this experiment.

[0085] With reference to the Guidelines for Field Efficacy Tests of Pesticides (I) Herbicides for Control of Weeds in Wheat Crops (GB / T 17980.41-2000), a certain amount of the test agent was diluted with 30L of water per mu, shaken and evenly sprayed on the ground or plant surface before wheat seedlings after sowing, during the three-leaf stage to tillering stage, and during the jointing stage to booting stage. Each treatment was repeated 4 times.

[0086] The mass percentages of isoproturon, palmitic acid, ferulic acid and adjuvants in the tested herbicidal compositions are shown in Table 3. The test set up isoproturon 100.00 g ai / mu, palmitic acid 15.00 g ai / mu and ferulic acid 15.00 g ai / mu as the drug control, and the blank control was not sprayed. 30 days after the spraying, diagonal sampling was adopted, and 5 sampling points (0.25m 2 / point), pull out all the Phragmites australis in the sample plot, first record the number of surviving Phragmites australis, then remove the dead branches and leaves, clean them and dry them, and weigh the biomass of Phragmites australis.

[0087] Table 3 Mass percentage of each component of the herbicidal composition containing isoproturon, palmitic acid and ferulic acid

[0088]

[0089] Table 4 Field control effect of the herbicidal composition of the invention on Phalaenopsis sphaerocephala at different stages

[0090]

[0091] Note: The lowercase letters in the table are for comparison of data in the same column. Different numbers indicate significant differences (P < 0.05), otherwise the differences are not significant.

[0092] As shown in Table 4, the herbicidal composition of the present invention, when applied after wheat sowing and before wheat seedlings, has significantly lower plant control efficiency and biomass control efficiency against Phalaenopsis spp. than a single agent of isoproturon (100.00 g ai / mu). However, in the wheat three-leaf stage to tillering stage and wheat jointing stage to booting stage, the herbicidal composition of the present invention has significantly higher plant control efficiency and biomass control efficiency against Phalaenopsis spp. than the three single agents of isoproturon, palmitic acid and ferulic acid.

[0093] When the herbicidal composition of the present invention is used at an amount of 25.00 g ai per mu of isoproturon, the plant control effect of the herbicidal composition on Phalaenopsis sibiricum is higher than 70% and the biomass control effect is higher than 80% during the three-leaf stage to tillering stage of wheat; the plant control effect of the herbicidal composition on Phalaenopsis sibiricum is higher than 60% and the biomass control effect is higher than 80% during the jointing stage to booting stage. When the chemical herbicide is used at 100 g ai / mu, the plant control effect and fresh weight control effect of the herbicidal composition on Phalaenopsis sibiricum are 52.17%, 51.19%, 59.51% and 56.49% respectively. The above results show that the herbicidal composition of the present invention has a low control effect on Phalaenopsis sibiricum when applied before wheat seedlings after sowing, so it is not recommended to apply the herbicidal composition before wheat seedlings after sowing; however, it has a high control effect on Phalaenopsis sibiricum in wheat fields by spraying the stems and leaves during the three-leaf stage to tillering stage and the jointing stage to booting stage of wheat, and it is recommended to apply it during this period.

[0094] Example 5: Safety evaluation of a herbicidal composition containing isoproturon, palmitic acid and ferulic acid on wheat when applied at different stages in wheat fields

[0095] 5.1 Test materials

[0096] The dosage and method of applying a herbicidal composition containing isoproturon, palmitic acid and ferulic acid in wheat fields at different stages were the same as those in Example 4. The wheat variety tested was Yunmai No. 8, a main variety grown in Yunnan Province.

[0097] 5.2 Test methods

[0098] The test method, test treatment and implementation series were the same as those in Example 4. The safety of the drug to wheat was investigated according to the 5-level classification method 3, 7, 15, 30, 45 and 60 days after administration.

[0099] 5-level classification standard for pesticide damage:

[0100] Level 0: Plants grow normally without any damage symptoms; recorded as “-”;

[0101] Level 1: less than 1 / 4 of the total leaves are dead; the plant is slightly damaged by the pesticide and can recover, which is recorded as "+";

[0102] Level 2: 1 / 4 to 1 / 2 of the total leaves are dead, the plant is moderately damaged by the pesticide, but can recover later and the yield is not affected; recorded as "++";

[0103] Level 3: 1 / 2 to 3 / 4 of the total leaves are dead, the plants are severely damaged and difficult to recover, resulting in reduced yield; recorded as "+++";

[0104] Level 4: More than 3 / 4 of the total leaf area or the entire plant is dead, the plant is seriously damaged by pesticides and is difficult to recover or the plant dies; recorded as "++++";

[0105] Table 5 Safety evaluation of rice at different times after different treatments

[0106]

[0107] The results of the study showed that the herbicidal composition containing isoproturon, palmitic acid and ferulic acid of the present invention was applied during the three-leaf stage to tillering stage and the jointing stage to booting stage of wheat. No abnormality or phytotoxicity was observed in wheat 3 days, 7 days, 15 days, 30 days, 45 days and 60 days after the application, indicating that the herbicidal composition of the present invention is safe for wheat. However, the control agent isoproturon was applied at a dosage of 100.00 g ai / mu during the three-leaf stage to tillering stage and the jointing stage to booting stage of wheat, and the phytotoxicity was serious after the application, and the wheat in the treatment group died completely. The other two control agents, palmitic acid and ferulic acid, were safe and harmless to wheat when applied at different stages of wheat. In summary, the herbicidal composition of the present invention is safe and harmless to wheat when applied by stem and leaf spraying during the three-leaf stage to tillering stage and the jointing stage to booting stage of wheat.

[0108] Example 6: Effect of applying a herbicidal composition containing isoproturon, palmitic acid and ferulic acid at different stages in wheat fields on wheat growth

[0109] 6.1 Test materials

[0110] The dosage and method of applying a herbicidal composition containing isoproturon, palmitic acid and ferulic acid in wheat fields at different stages were the same as those in Example 4. The wheat variety tested was Yunmai No. 8, a main variety grown in Yunnan Province.

[0111] 6.2 Test methods

[0112] The test method, test treatment and implementation series are the same as Example 4.

[0113] 6.3 Test results

[0114] Table 6 Effect of a herbicidal composition containing isoproturon, palmitic acid and ferulic acid on wheat growth when applied at different wheat stages

[0115]

[0116] Note: The lowercase letters in the table are for comparison of data in the same industry. Different numbers indicate significant differences (P < 0.05), otherwise the differences are not significant.

[0117] As shown in Table 6, in the blank control treatment, the effective ear, number of grains, thousand-grain weight and yield of wheat were 489.1, 28.6, 38.1g and 355.48 Kg / mu, respectively. Different components of the herbicide composition containing isoproturon, palmitic acid and ferulic acid were applied in the three-leaf stage to the tillering stage and the jointing stage to the booting stage of wheat. The effective ear, number of grains, thousand-grain weight and yield of wheat in all treatment groups were higher than the blank control and the single-dose control of isoproturon, palmitic acid and ferulic acid. Among them, after the treatment of the three-leaf stage to the tillering stage, the yield increase rate of wheat was higher than that of the jointing stage to the booting stage under the same conditions. However, except for series III, there was no significant difference in the yield increase of wheat under the same conditions when the pesticides were applied at different periods. In addition, the study also showed that when isoproturon was 100 g ai / mu, the application of the pesticide in the three-leaf stage to the tillering stage and the jointing stage to the booting stage of wheat caused the death of wheat. The above results show that a herbicide composition containing isoproturon, palmitic acid and ferulic acid can significantly increase wheat yield when applied during the three-leaf stage to tillering stage and the jointing stage to booting stage of wheat.

Claims

1. A herbicidal composition for controlling Phalaenopsis sphaerocephala, characterized in that: The following raw materials are included in mass percentage: Isoproturon 25.00~35.00%; Palmitic acid 2.50~12.50%; Ferulic acid 2.50~12.50%; Additives 2.50~7.00%; Deionized water in appropriate amount.

2. The herbicidal composition for controlling Phalaenopsis sphaerocephala as claimed in claim 1, characterized in that: The auxiliary agent is composed of ethoxy-modified trisiloxane, dodecyl diphenyl ether disulfonate and epoxy soybean oil, and the mass percentage of each component is: Ethoxy modified trisiloxane 1.00~3.00%; Dodecyl diphenyl ether disulfonate 1.00~3.00%; Epoxidized soybean oil 0.50~1.00%.

3. A method for preparing the herbicidal composition for controlling Phalaenopsis sphaerocephala as claimed in claim 1, characterized in that: The following steps are involved: S1, add chloroisopropuron to the reaction tank a according to the ratio, stir evenly, inject the composition into the multi-stage grinder for grinding, and discharge the ground material after 98% of the fineness passes 2-4 um, and the material a is set aside; S2, adding palmitic acid, ferulic acid, ethoxy-modified trisiloxane, dodecyl diphenyl ether disulfonate and epoxy soybean oil into reaction tank b according to the proportion, and stirring until completely mixed; S3, injecting material a and the material in reaction tank b into an emulsification tank, adding deionized water according to the proportion, and then high-speed shearing and emulsification to obtain a herbicidal composition for controlling Phalaenopsis calyx.

4. The method for preparing the herbicidal composition for controlling Phalaenopsis sphaerocephala as claimed in claim 3, characterized in that: The distribution ratios of each group are as follows: Isoproturon 25.00~35.00%; Palmitic acid 2.50~12.50%; Ferulic acid 2.50~12.50%; Additives 2.50~7.00%; Ethoxy modified trisiloxane 1.00~3.00%; Dodecyl diphenyl ether disulfonate 1.00~3.00%; Epoxidized soybean oil 0.50~1.00%; Add deionized water to 100.00%.

5. Use of the herbicidal composition for controlling Pseudostellaria spp. according to any one of claims 1 to 2 or the herbicidal composition prepared by the preparation method of the herbicidal composition for controlling Pseudostellaria spp. according to any one of claims 3 to 4, characterized in that: During the three-leaf stage to tillering stage before wintering of wheat or the jointing stage to booting stage after wintering, choose a clear and windless afternoon or evening, and evenly spray the herbicidal composition at a rate of 100 g per mu diluted with 30 kg of water on the plant surface at one time.

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