A multifunctional integrated shaking incubator based on cell culture and method

Abstract

The present invention discloses a multifunctional oscillating culture integrated shaker and method based on cell culture, which relates to the technical field of enzyme biological cells. A split oscillating platform for placing containers is configured inside the body. The oscillating platform is composed of multiple oscillating plates, and clamping members are configured on the upper part of the oscillating plates to clamp the containers. A driving module connected to the oscillating platform is configured inside the body. The driving module includes an oscillating unit and a connecting unit. The connecting unit connects the oscillating unit and the oscillating plates. The connecting unit is configured with two transmission states for adjacent oscillating plates through a differential, and the differential is configured to drive multiple oscillating plates to oscillate at the same speed or at different speeds by the driving module. In this application, the oscillating platform is split into multiple oscillating plates. When oscillating enzyme biological cells, the enzyme biological cells that require different oscillating parameters are placed on different oscillating plates, and multiple enzyme biological cells can be oscillated in the same shaker through oscillating plates with different oscillating frequencies.

Description

Technical Field

[0001] The present invention relates to the technical field of enzyme biological cells, and specifically to an integrated shaker and method for enzyme activity determination, enzyme reaction experiments, enzyme biological cell separation, purification, and culture through oscillation. Background Technique

[0002] Constant temperature shakers have extensive applications in enzyme biological research, mainly including the following aspects:

[0003] The constant temperature shaker measures enzyme activity at a constant temperature to ensure the stability of experimental conditions, thereby obtaining accurate measurement results.

[0004] When conducting enzyme-catalyzed reaction experiments, the constant temperature shaker provides constant temperature and oscillation conditions, which helps to evenly mix the enzyme and the substrate and improve the reaction efficiency.

[0005] Oscillation can promote the separation of enzymes from other proteins and improve the purification efficiency.

[0006] By maintaining constant temperature and oscillation conditions, the storage period of the enzyme can be extended and its activity can be maintained.

[0007] Publication No. CN114774276B discloses a constant temperature shaker core component and a constant temperature shaker, including a bottom plate, a shaking plate, an eccentric drive mechanism, and a follower mechanism. Among them, the shaking plate is arranged directly above the bottom plate; the eccentric drive mechanism is arranged on the bottom plate and can drive the shaking plate to move. The output end of the eccentric shaft of the eccentric drive mechanism is rotationally connected to the first bearing seat on the shaking plate through a bearing; the follower mechanism includes a translation component, a side swing rod, and a connecting rod. The translation component is located between the bottom plate and the shaking plate and can move horizontally and / or longitudinally between the two; there are two side swing rods arranged in parallel. One end of the side swing rod is hinged to the first side of the translation component; the connecting rod is hinged to the other ends of the two side swing rods to form a parallelogram, and the connecting rod is fixedly connected to the first bearing seat. Through the design of the follower mechanism, it can only translate and cannot rotate, thereby preventing the self-rotation of the first bearing seat during the operation of the shaker.

[0008] The shaker is driven by a motor and uses a transmission mechanism to drive the movement of the shaker platform. Common transmission mechanisms include:

[0009] Crank and connecting rod mechanism: Converts rotational motion into horizontal reciprocating motion.

[0010] Eccentric wheel mechanism: Drives the platform to perform circular or elliptical oscillating motion through the rotation of the eccentric wheel.

[0011] Ball screw: Utilizes the rotation of the screw to drive the platform to move in a straight line direction and is suitable for shakers with precise control.

[0012] However, for the above driving methods, there are some corresponding problems. Since the heights of the internal liquids are different and the containers for holding the liquids are different, and the stability of the liquid during oscillation by the above mechanical driving method is poor, therefore, specific containers and liquid level control are required during oscillation. Summary of the Invention

[0013] One of the objectives of the present invention is to provide a multifunctional oscillating culture integrated shaker and method based on cell culture, which controls the oscillation parameters of the oscillation platform by obtaining the state of the liquid on the oscillation platform, avoids the liquid inside the container from spilling due to excessive oscillation parameters, and more precisely controls the state of the liquid according to the container.

[0014] To achieve the above objectives, the present invention is realized through the following technical solutions:

[0015] A multifunctional oscillating culture integrated shaker based on cell culture, comprising a body that can be placed on the ground and is configured with an observation window. Inside the body, there is a split-type oscillation platform for placing containers. The oscillation platform is composed of multiple oscillation plates, and clamping members are arranged on the upper part of the oscillation plates to clamp the containers.

[0016] A driving module connected to the oscillation platform is arranged inside the body. The driving module includes an oscillation unit and a connection unit. The connection unit connects the oscillation unit and the oscillation plates. The connection unit configures two transmission states for adjacent oscillation plates through a differential. The differential is configured to drive multiple oscillation plates to oscillate at the same speed or at different speeds by the driving module.

[0017] A collection device is installed inside the body to collect the liquid level change of the container during oscillation.

[0018] In one or more embodiments of the present invention, the body further includes a retractable cover. A cover for observation and sealing is hinged to the outside of the cover, and the cover can swing open.

[0019] The cover can be retractably installed inside the body, and a clamping plate is arranged on the outside of the body to support and limit the cover. When the cover is retracted, the volume of the shaker is smaller than the volume of the shaker when the cover is supported.

[0020] In one or more embodiments of the present invention, a first connecting member, a second connecting member, and a transmission member connecting the first connecting member and the second connecting member and adjusting the transmission ratio between the first connecting member and the second connecting member are arranged inside the differential housing.

[0021] The first connecting member and the second connecting member are respectively installed at the bottoms of adjacent two oscillation plates, and the movement of adjacent two oscillation plates is realized in a manner that the first connecting member is used as the driving member and the second connecting member is used as the driven member.

[0022] In one or more embodiments of the present invention, the transmission member includes:

[0023] The first slider and the second slider are both arranged inside the differential, and are respectively connected to the first connecting piece and the second connecting piece, and the first connecting piece and the second connecting piece move synchronously with the first slider and the second slider;

[0024] The connecting pipeline is arranged inside the differential housing, and the first slider and the second slider slide to change the medium inside the connecting pipeline;

[0025] The pressing plate is arranged inside the differential housing. One end of the pressing plate facing away from the first slider or the second slider is configured with a magnetic component to adjust the position of the pressing plate. The change in the position of the pressing plate changes the cross-sectional area of the chute of the first slider or the second slider. When the cross-sectional area of the pressing plate changes, the cross-sectional area of the end of the first slider and the second slider extending to the inner side of the chute changes accordingly. The connecting pipeline is connected to the chute.

[0026] In one or more embodiments of the present invention, both the first slider and the second slider are provided with a folding plate supported by a compression spring and attached to the outer wall of the pressing plate. When the pressing plate is squeezed, the folding plate is also squeezed, and the pressing plate and the folding plate move synchronously;

[0027] An inlet and an outlet are provided on both sides of the chute where the first slider and the second slider are installed. The inlet and outlet on one side cannot be opened simultaneously. A seal is arranged outside the chute and moves along the movement direction of the first slider or the second slider. The seal extends into the chute to seal the inlet or the outlet, and the outlets or inlets on both sides of the chute cannot be opened simultaneously;

[0028] The connecting pipeline connects the outlet of the chute where the first slider is installed to the inlet of the chute where the second slider is installed, and the inlet of the chute where the first slider is installed to the outlet of the chute where the second slider is installed.

[0029] In one or more embodiments of the present invention, both the first connecting piece and the second connecting piece include:

[0030] The connecting plate is installed at the bottom of the oscillating plate through a connecting structure. One end of the connecting plate is configured with a connecting rod to connect the first slider and the connecting plate or the second slider and the connecting plate.

[0031] In one or more embodiments of the present invention, the first connecting piece and the second connecting piece are set as a four-bar mechanism, and the linear reciprocating motion of the first slider or the second slider is converted into a circular motion through the four-bar mechanism.

[0032] In one or more embodiments of the present invention, the clamping member includes:

[0033] The placement rack is installed on the upper part of the oscillating plate. An arc-shaped soft plate is configured inside the placement rack. Magnets are arranged at both ends of the arc-shaped soft plate. The cross-sectional area of the arc-shaped soft plate formed by the magnetic attraction and contraction between adjacent magnets;

[0034] An elastomer is fixed to one side of the arc-shaped flexible plate that fits the container, and the inside of the elastomer is provided with a cavity.

[0035] In one or more embodiments of the present invention, the oscillation unit includes:

[0036] An electromagnet is fixed inside the body. A guide frame is arranged on the outer wall of the oscillation plate close to the electromagnet, and a guide magnet is arranged inside the guide frame, and the guide magnet moves inside the guide frame;

[0037] Positioning magnets are fixed at both ends of the inner wall of the guide frame to support the guide magnet and prevent the guide magnet from contacting the positioning magnets.

[0038] This application also provides an integrated shaker oscillation method for the above integrated shaker, including the following steps:

[0039] Determine the oscillation frequency of the enzyme biological cells to be oscillated, and place the container inside the clamping member;

[0040] The oscillation unit drives one of the oscillation plates to oscillate, and the oscillation plate is connected to other oscillation plates through the connection unit to synchronously oscillate;

[0041] Adjust the oscillation frequency of each oscillation plate through the differential;

[0042] During the oscillation process, the acquisition device obtains the container liquid level image during the oscillation process, and analyzes the container liquid level image to determine the fluctuation of the liquid level during the oscillation.

[0043] Through the above technical solutions, the present invention has the following beneficial effects:

[0044] 1. In this application, the oscillation platform is split into multiple oscillation plates. When oscillating enzyme biological cells, place the enzyme biological cells that require different oscillation parameters on different oscillation plates, and it is possible to oscillate multiple enzyme biological cells in the same shaker through oscillation plates with different oscillation frequencies, further improving the adaptability of the shaker.

[0045] 2. By using a differential with adjustable differential, it is possible to dynamically adjust the transmission ratio of the transmission between the first connecting member and the second connecting member, so that the two oscillation plates connected by the first connecting member and the second connecting member can generate different oscillation frequencies, enabling multiple oscillation plates to oscillate synchronously but presenting different oscillation frequencies.

[0046] 3. The setting of the differential can achieve the same-speed movement and differential movement of two adjacent oscillation plates, that is, arbitrarily adjust the oscillation frequencies of two adjacent oscillation plates, so as to be able to change the vibration frequencies of enzyme biological cells at different positions and perform oscillations with multiple vibration frequencies in the same shaker.

[0047] 4. By using a collection device to collect the liquid level state of the container during the oscillation of the shaker, it is possible to determine whether the liquid will spill out at the vibration frequency. Through the collected images of the collection device, the oscillation frequency of the containers that may spill is reduced to ensure the stability of the liquid inside the containers.

[0048] Other features and advantages of the present invention will be described in the subsequent specification, and part of them will become obvious from the specification or be understood by implementing the present invention. The objectives and other advantages of the present invention can be achieved and obtained through the structures specifically pointed out in the written specification and the drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 is a perspective view of the present invention;

[0050] Figure 2 is a schematic diagram of the internal structure of the present invention;

[0051] Figure 3 is a schematic diagram of the structure of the present invention with the clamping member removed;

[0052] Figure 4 is a schematic diagram of the oscillation platform of the present invention;

[0053] Figure 5 is a schematic diagram of the bottom of the oscillation platform of the present invention;

[0054] Figure 6 is a schematic diagram of the oscillation plate of the present invention;

[0055] Figure 7 is a schematic diagram of the connection unit of the present invention;

[0056] Figure 8 is a cross-sectional view of the differential of the present invention;

[0057] Figure 9 is an exploded view of the internal structure of the differential of the present invention;

[0058] Figure 10 is a schematic diagram of a partial structure of the differential of the present invention;

[0059] Figure 11 is a schematic diagram of the pressing plate structure of the present invention;

[0060] Figure 12 is an exploded view of the structure of the first slider or the second slider of the present invention;

[0061] Figure 13 is a schematic diagram of the internal structure of the guiding structure of the present invention;

[0062] Figure 14 is a schematic diagram of the clamping member structure of the present invention;

[0063] Figure 15 Schematic diagram of the arc flexible board structure of the present invention

[0064] In the figure: 1 body, 2 oscillation platform, 3 clamping member, 4 drive module, 5 oscillation unit, 6 connection unit, 7 acquisition device;

[0065] 21 oscillation plate, 22 cover body, 23 cover, 24 clamping plate;

[0066] 31 placement rack, 32 arc flexible board, 33 magnet, 34 elastic body;

[0067] 51 electromagnet, 52 guide frame, 53 guide magnet, 54 positioning magnet;

[0068] 61 first connection member, 62 second connection member, 63 transmission member;

[0069] 611 connection plate, 612 connection rod;

[0070] 631 first slider, 632 second slider, 633 chute, 634 communication pipeline, 635 pressing plate, 636 magnetic component, 637 folding plate, 638 compression spring, 639 inlet, 6310 outlet, 6311 seal; Specific embodiments

[0071] The following will disclose multiple embodiments of the present invention with the accompanying drawings. For the sake of clarity, many practical details will be described together in the following narrative. However, it should be understood that these practical details are not used to limit the present invention. That is to say, in some embodiments of the present invention, these practical details are not necessary. And if possible in implementation, the features of different embodiments can be interactively applied.

[0072] Unless otherwise defined, all terms (including technical and scientific terms) used herein have their ordinary meanings, and their meanings can be understood by those skilled in this field. Further, the definitions of the above terms in commonly used dictionaries should be interpreted as having the same meaning as the relevant fields of the present invention in the content of this specification. Unless specifically defined, these terms will not be interpreted as idealized or overly formal meanings.

[0073] The following explains the relationships and terms used in this application:

[0074] Parallel: The parallel defined in this application is not limited to absolute parallel. This definition of parallel can be understood as substantially parallel, allowing for situations where it is not absolutely parallel due to factors such as assembly tolerances, design tolerances, and the influence of structural flatness. Small-angle range errors are allowed, for example, within an assembly error range of less than 10 degrees, and it can be understood as a parallel relationship.

[0075] Vertical: The vertical defined in this application is not limited to an absolutely vertically intersecting (with an included angle of 90 degrees) relationship. A relationship that is not an absolutely vertically intersecting one caused by factors such as assembly tolerances, design tolerances, and the influence of structural flatness is allowed, and an error within a small angle range is permitted. For example, within an assembly error range of 80 degrees to 100 degrees, it can all be understood as a vertical relationship.

[0076] Ground: The ground defined in this application is not limited to the ground of a certain material or region. It is only a platform for carrying this application on the surface, and stacking, tilting, and flatness changes are allowed. For example, a cement ground, a ceramic tile ground, a working platform, etc. can all be interpreted as the ground.

[0077] The above explanations do not fully cover the relationship definitions given in this application and only represent a part of this application.

[0078] The present invention provides a multifunctional oscillating culture integrated shaker based on cell culture, which is used for oscillating enzyme biological cells, and determines the maximum oscillation parameters according to the oscillation reaction of the liquid under different parameters during the oscillation process.

[0079] Refer to Figure 1-3 As shown, in one embodiment, the integrated shaker includes a body 1 that can be placed on the ground and is configured with an observation window. Inside the body 1, there is a split-type oscillating platform 2 for placing containers. The oscillating platform 2 is composed of a plurality of oscillating plates 21. A clamping member 3 is arranged on the upper part of the oscillating plate 21 to clamp the container.

[0080] Inside the body 1, there is a driving module 4 connected to the oscillating platform 2. The driving module 4 includes an oscillating unit 5 and a connecting unit 6. The connecting unit 6 connects the oscillating unit 5 and the oscillating plate 21. The connecting unit 6 configures two transmission states for adjacent oscillating plates 21 through a differential, and the differential is configured to drive a plurality of oscillating plates 21 to oscillate at the same speed or at different speeds under the drive of the driving module 4.

[0081] A collection device 7 is installed inside the body 1 to collect the liquid level change of the container during the oscillation process.

[0082] In an implementable manner, through the setting of the connecting unit 6, a plurality of oscillating plates 21 are connected, enabling the plurality of oscillating plates 21 to move synchronously. Clamping members 3 are arranged on the upper parts of the plurality of oscillating plates 21 to clamp the container, ensuring the stability of the container during the oscillation process.

[0083] Optionally, the body 1 includes a control system for adjusting oscillation parameters, temperature, humidity, and oscillation time. The control system is integrated inside the body 1, and a temperature control system is configured inside the body 1 to control the temperature change inside the body 1.

[0084] Among them, since the oscillation frequencies required by enzyme biological cells in different states are different, and due to the differences in the container and the liquid inside it, the same oscillation frequency will have different effects. To avoid the problem that the shaker can only set one vibration frequency, the oscillation platform 2 is set to be composed of a plurality of oscillation plates 21, and they are connected to each other through a connecting unit 6, enabling differential oscillation between the oscillation plates 21.

[0085] Refer to Figure 1-3 As shown, in one embodiment, the body 1 further includes a retractable cover 22. A cover 23 for observation and sealing is hinged to the outside of the cover 22, and the cover 23 can swing open.

[0086] The cover 22 can be retractably installed inside the body 1, and a clamping plate 24 is provided on the outside of the body 1 to support and limit the cover 22. When the cover 22 is retracted, the volume of the shaker is smaller than that when the cover 22 is supported.

[0087] In an implementable manner, the retractable cover 22 is used to reduce the volume of the shaker when it is not in use, facilitating the carrying and transportation of the shaker. After the cover 22 is unfolded, the clamping plate 24 supports the cover 22 to ensure the stability of the cover 22.

[0088] Optionally, the clamping plate 24 is swingably arranged. One end of the clamping plate 24 is hinged to the body 1, and the other end is provided with an opening. A protrusion extends outward from the cover 22 corresponding to the position of the clamping plate 24. When the clamping plate 24 is perpendicular to the ground and the opening faces the setting, it supports the cover 22.

[0089] In another option, the clamping plate 24 is swingably arranged. One end of the clamping plate 24 is installed on the outside of the body 1 through a rotating shaft and can rotate relative to the body 1, and the other end is provided with a groove. A protrusion extends outward from the cover 22 corresponding to the position of the clamping plate 24. When the protrusion enters the groove, the clamping plate 24 supports the cover 22.

[0090] In another option, the clamping plate 24 is independently arranged. A clamping groove capable of placing the clamping plate 24 is configured inside the body 1. One end of the clamping plate 24 is inserted into the inside of the clamping groove and the other end is provided with an opening or a groove. The protrusion on the outside of the cover 22 cooperates with the opening or the groove to ensure the stability of the cover 22. Setting the clamping plate 24 independently requires additional storage of the clamping plate 24 compared with the way the clamping plate 24 is connected to the body 1, but it reduces wear compared with the way the clamping plate 24 is connected to the body 1.

[0091] Refer to Figure 4-6 As shown, in one embodiment, a first connecting piece 61, a second connecting piece 62, and a transmission piece 63 that connects the first connecting piece 61 and the second connecting piece 62 and adjusts the transmission ratio between the first connecting piece 61 and the second connecting piece 62 are arranged inside the differential housing.

[0092] The first connecting member 61 and the second connecting member 62 are respectively installed at the bottoms of two adjacent oscillating plates 21 and are configured to enable the two adjacent oscillating plates 21 to move in such a way that the first connecting member 61 is the driving member and the second connecting member 62 is the driven member.

[0093] In an implementable manner, when the first connecting member 61 moves, it drives the transmission member 63 to change. The transmission member 63 drives the second connecting member 62 to act according to the transmission ratio. Multiple oscillating plates 21 are all connected through a differential. In one differential, the side close to the driving unit is the first connecting member 61.

[0094] Under the connection of the differential, the adjacent oscillating plates 21 have the same motion mode, that is:

[0095] When the oscillating plate 21 connected to the first connecting member 61 performs a linear reciprocating motion, the oscillating plate 21 connected to the second connecting member 62 also performs a linear reciprocating motion;

[0096] When the oscillating plate 21 connected to the first connecting member 61 performs a circular motion, the oscillating plate 21 connected to the second connecting member also performs a circular motion.

[0097] Refer to Figure 7-10 As shown, in one embodiment, the transmission member 63 includes:

[0098] A first slider 631 and a second slider 632, both arranged inside the differential, are respectively connected to the first connecting member 61 and the second connecting member 62, and the first connecting member 61 and the second connecting member 62 move synchronously with the first slider 631 and the second slider 632;

[0099] A communication pipeline 634, arranged inside the differential housing, and the first slider 631 and the second slider 632 slide to change the medium inside the communication pipeline 634;

[0100] A pressing plate 635, arranged inside the differential housing. One end of the pressing plate 635 facing away from the first slider 631 or the second slider 632 is configured with a magnetic assembly 636 to adjust the position of the pressing plate 635. The change in the position of the pressing plate 635 changes the cross-sectional area of the chute 633 of the first slider 631 or the second slider 632. When the cross-sectional area of the pressing plate 635 changes, the cross-sectional area of the end of the first slider 631 and the second slider 632 extending to the inside of the chute 633 changes accordingly, and the communication pipeline 634 communicates with the chute 633.

[0101] In an implementable manner, the first slider 631 slides to change its position to drive the medium arranged inside the chute 633 and the communication pipeline 634 to move, and the second slider 632 is driven to move through the movement of the medium. After the cross-sectional area of the chute 633 is adjusted by the pressing plate 635, the first slider 631 and the second slider 632 are adjusted correspondingly.

[0102] Exemplarily, the cross-sectional area of the chute 633 where the second slider 632 is installed is reduced. With the cross-sectional area of the chute 633 of the first slider 631 remaining unchanged, when the second slider 632 and the first slider 631 move the same distance, the medium required to drive the second slider 632 is less than that required for the first slider 631, thereby achieving differential motion between the first slider 631 and the second slider 632. According to the changes in the corresponding cross-sectional areas of the first slider 631 and the second slider 632, synchronous or differential speeds are achieved.

[0103] Among them, the magnetic component 636 is an electromagnet.

[0104] Refer to Figure 11-12 As shown, in one embodiment, a flap 637 supported by a compression spring 638 and attached to the outer wall of the pressure plate 635 is provided on both the first slider 631 and the second slider 632. When the pressure plate 635 is squeezed, the flap 637 is also squeezed, and the pressure plate 635 and the flap 637 move synchronously;

[0105] An inlet 639 and an outlet 6310 are provided on both sides of the chute 633 where the first slider 631 and the second slider 632 are installed. The inlet 639 and the outlet 6310 on one side cannot be opened simultaneously. A seal 6311 that moves along the moving direction of the first slider 631 or the second slider 632 is configured outside the chute 633. The seal 6311 extends into the chute 633 to seal the inlet 639 or the outlet 6310, and the outlets 6310 or inlets 639 on both sides of the chute 633 cannot be opened simultaneously;

[0106] The connecting pipeline 634 connects the outlet 6310 of the chute 633 where the first slider 631 is installed to the inlet 639 of the chute 633 where the second slider 632 is installed, and the inlet 639 of the chute 633 where the first slider 631 is installed to the outlet 6310 of the chute 633 where the second slider 632 is installed.

[0107] In an implementable manner, in order to clarify the states of the outlet 6310 and the inlet 639, a check valve is configured at one end of the connecting pipeline 634 that connects to the inlet 639 or the outlet 6310, that is, the inlet 639 can only function as an inlet, and the outlet 6310 can only function as an outlet, avoiding the medium on the left side of the chute 633 entering the right side through the connecting pipeline 634 when the first slider 631 slides and being unable to drive the second slider 632 to move.

[0108] Since the driving speed of the first slider 631 is a fixed value, therefore, the speed at which the medium enters the second slider 632 is also a fixed value. With the cross-sectional area of the chute 633 adjusted, differential speed of the second slider 632 is achieved.

[0109] Among them, a sealing member 6311 is correspondingly installed in one chute 633. When the first slider 631 or the second slider 632 moves to one end of the chute 633, the sealing member 6311 will be pushed to displace, and after the displacement, the sealing member 6311 will seal the outlets 6310 or inlets 639 on both sides of the chute 633. Exemplarily, when the first slider 631 moves leftward from the middle position, the outlet 6310 on the left side of the chute 633 is opened, and the inlet 639 on the right side of the chute 633 is opened. When the first slider 631 moves to the leftmost position, the sealing member 6311 is pushed to displace, and the sealing member 6311 seals the outlet 6310, making the left inlet 639 opened and the right outlet 6310 opened. At this time, the first slider 631 starts to move rightward.

[0110] In another embodiment, the transmission member 63 forms different transmission ratios through the meshing of sliding gears to achieve the transmission between the first connecting member 61 and the second connecting member 62. However, compared with the transmission method through the above-mentioned transmission member 63, on the one hand, the structure of the differential will increase, and on the other hand, since the differential is gear transmission, the noise generated during the gear transmission will be relatively large. And the gear transmission method will increase the overall weight of the shaker.

[0111] Refer to Figure 8-10 As shown, in one embodiment, the first connecting member 61 and the second connecting member 62 both include:

[0112] A connecting plate 611, which is installed at the bottom of the oscillating plate 21 through a connecting structure. One end of the connecting plate 611 is configured with a connecting rod 612 to connect the first slider 631 and the connecting plate 611 or the second slider 632 and the connecting plate 611.

[0113] In an implementable way, connecting the transmission member 63 and the oscillating plate 21 through the first connecting member 61 and the second connecting member 62 can ensure the synchronous movement of the transmission member 63 and the oscillating plate 21, and ensure the stability during the movement process.

[0114] In one embodiment, the first connecting member 61 and the second connecting member 62 are arranged as a four-bar mechanism, which converts the linear reciprocating motion of the first slider 631 or the second slider 632 into a circular motion.

[0115] In an implementable way, through the arrangement of the four-bar mechanism, the movement state of the oscillating plate 21 can be adjusted, so that the oscillating plate 21 can generate different movement states.

[0116] Refer to Figure 14-15 As shown, in one embodiment, the clamping member 3 includes:

[0117] The placement rack 31 is installed above the oscillation plate 21. An arc-shaped flexible plate 32 is arranged inside the placement rack 31. Magnetic blocks 33 are arranged at both ends of the arc-shaped flexible plate 32. The cross-sectional area formed by the arc-shaped flexible plate 32 is magnetically absorbed and contracted between adjacent magnetic blocks 33.

[0118] The elastic body 34 is fixed on the side of the arc-shaped flexible plate 32 that fits the container, and the inside of the elastic body 34 is provided with a cavity.

[0119] In an implementable manner, the magnetic attraction of the magnetic block 33 can contract the arc-shaped flexible plate 32. When fixing the container, the arc-shaped flexible plate 32 is contracted so that the elastic body 34 inside the arc-shaped flexible plate 32 fits the surface of the container. The elastic body 34 is made of rubber material, and most of the container is made of glass material. The friction with the glass is increased through the rubber material, and further the stability of the container during the oscillation process is improved.

[0120] Refer to Figure 7 and 13 As shown, in an embodiment, the oscillation unit 5 includes:

[0121] The electromagnet 51 is fixed inside the machine body 1. A guide frame 52 is arranged on the outer wall of the oscillation plate 21 close to the electromagnet. A guide magnet 53 is arranged inside the guide frame 52, and the guide magnet 53 moves inside the guide frame 52.

[0122] The positioning magnets 54 are fixed at both ends of the inner wall of the guide frame 52 to support the guide magnet 53 and prevent the guide magnet 53 from contacting the positioning magnets.

[0123] In an implementable manner, the guide magnet 53 is controlled by the electromagnet. The electromagnets 51 are located on both sides of the guide frame 52. The guide magnet 53 is pushed by the magnetic force of the electromagnets 51 to generate inertia, and the oscillation plate 21 is moved in one direction through the inertia. When the guide magnet 53 moves to the other end, the electromagnet 51 on the other side pushes the guide magnet 53 to move in the reverse direction.

[0124] This application also provides an integrated shaker oscillation method for the above integrated shaker, including the following steps:

[0125] Determine the oscillation frequency of the enzyme biological cells to be oscillated, and place the container inside the clamping member 3;

[0126] The oscillation unit 5 drives one of the oscillation plates 21 to oscillate, and the oscillation plates 21 are connected by the connection unit 6 to oscillate synchronously;

[0127] Adjust the oscillation frequency of each oscillation plate 21 through the differential;

[0128] During the oscillation process, the acquisition device 7 acquires the image of the liquid level of the container during the oscillation process, and analyzes the image of the liquid level of the container to determine the fluctuation of the liquid level during the oscillation process.

[0129] Although the present invention is disclosed in connection with the above embodiments, it is not intended to limit the present invention. Any person skilled in the art can make various modifications and refinements without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be defined by the appended claims.

Claims

1. A multifunctional oscillating and culturing integrated shaker based on cell culture, comprising a body (1) that can be placed on the ground and is configured with an observation window, characterized in that, Inside the body (1), a split oscillating platform (2) for placing containers is configured. The oscillating platform (2) is composed of multiple oscillating plates (21). Clamping members (3) are configured on the upper part of the oscillating plates (21) to clamp the containers. A drive module (4) connected to the oscillating platform (2) is configured inside the body (1). The drive module (4) includes an oscillating unit (5) and a connecting unit (6). The connecting unit (6) connects the oscillating unit (5) and the oscillating plates (21). The connecting unit (6) configures two transmission states for adjacent oscillating plates (21) through a differential. The differential is configured to drive multiple oscillating plates (21) to oscillate at the same speed or at different speeds by the drive module (4). Inside the differential housing, a first connecting member (61), a second connecting member (62), and a transmission member (63) connecting the first connecting member (61) and the second connecting member (62) and adjusting the transmission ratio between the first connecting member (61) and the second connecting member (62) are provided. The first connecting member (61) and the second connecting member (62) are respectively installed at the bottoms of two adjacent oscillating plates (21), and the movement of two adjacent oscillating plates (21) is realized in a way that the first connecting member (61) is used as the driving member and the second connecting member (62) is used as the driven member. The transmission member (63) includes: A first slider (631) and a second slider (632), both arranged inside the differential, respectively connecting the first connecting member (61) and the second connecting member (62), and the first connecting member (61) and the second connecting member (62) move synchronously with the first slider (631) and the second slider (632). A communicating pipeline (634) is configured inside the differential housing. The first slider (631) and the second slider (632) slide to change the medium inside the communicating pipeline (634). A pressing plate (635) is arranged inside the differential housing. A magnetic component (636) is configured at one end of the pressing plate (635) facing away from the first slider (631) or the second slider (632) to adjust the position of the pressing plate (635). When the position of the pressing plate (635) changes, the cross-sectional area of the chute (633) of the first slider (631) or the second slider (632) changes. When the cross-sectional area of the pressing plate (635) changes, the cross-sectional area of the end of the first slider (631) and the second slider (632) extending to the inner side of the chute (633) changes accordingly. The communicating pipeline (634) is communicated with the chute (633). A collecting device (7) is installed inside the body (1) to collect the liquid level change of the container during oscillation.

2. The multifunctional oscillating culture integrated shaker based on cell culture according to claim 1, wherein The body (1) further includes a retractable cover (22). A cover (23) for observation and sealing is hinged to the outside of the cover (22), and the cover (23) can swing open. The cover (22) can be retractably installed inside the body (1), and a clamping plate (24) is arranged on the outside of the body (1) to support and limit the cover (22). When the cover (22) is retracted, the volume of the shaker is smaller than the volume of the shaker when the cover (22) is supported.

3. The multifunctional integrated shaking incubator based on cell culture according to claim 2, wherein, A folding plate (637) supported by a compression spring (638) and attached to the outer wall of the pressing plate (635) is provided for both the first slider (631) and the second slider (632). When the pressing plate (635) is squeezed, the folding plate (637) is also squeezed, and the pressing plate (635) and the folding plate (637) move synchronously. An inlet (639) and an outlet (6310) are provided on both sides of the chute (633) where the first slider (631) and the second slider (632) are installed. The inlet (639) and the outlet (6310) on one side cannot be opened simultaneously. A seal (6311) moving in the moving direction of the first slider (631) or the second slider (632) is configured outside the chute (633). The seal (6311) extends to the chute (633) to seal the inlet (639) or the outlet (6310), and the outlets (6310) or inlets (639) on both sides of the chute (633) cannot be opened simultaneously. The connecting pipeline (634) connects the outlet (6310) of the chute (633) where the first slider (631) is installed to the inlet (639) of the chute (633) where the second slider (632) is installed, and the inlet (639) of the chute (633) where the first slider (631) is installed to the outlet (6310) where the second slider (632) is installed.

4. A multifunctional integrated shaking incubator based on cell culture according to claim 3, characterized in that, Both the first connecting member (61) and the second connecting member (62) include: A connecting plate (611) installed at the bottom of the oscillating plate (21) through a connecting structure. One end of the connecting plate (611) is configured with a connecting rod (612) to connect the first slider (631) and the connecting plate (611) or the second slider (632) and the connecting plate (611).

5. The integrated shaker for multifunctional oscillating culture based on cell culture according to claim 4, wherein, The first connecting member (61) and the second connecting member (62) are set as a four-bar mechanism, which converts the linear reciprocating motion of the first slider (631) or the second slider (632) into circular motion.

6. The multifunctional oscillating and culturing integrated shaker based on cell culture according to claim 3, characterized in that, The clamping member (3) includes: A placement rack (31) installed on the upper part of the oscillating plate (21). An arc-shaped soft plate (32) is configured inside the placement rack (31). Magnets (33) are provided at both ends of the arc-shaped soft plate (32), and the cross-sectional area formed by the arc-shaped soft plate (32) is magnetically attracted and contracted between adjacent magnets (33). An elastomer (34) is fixed on the side of the arc-shaped soft plate (32) that fits the container, and the inside of the elastomer (34) is provided with a cavity.

7. A multifunctional integrated shaking incubator based on cell culture according to claim 6, characterized in that, The oscillation unit (5) includes: An electromagnet (51) fixed inside the machine body (1). A guide frame (52) is configured on the outer wall of the oscillating plate (21) close to the electromagnet. A guide magnet (53) is provided inside the guide frame (52), and the guide magnet (53) moves inside the guide frame (52). Positioning magnets (54) are fixed at both ends of the inner wall of the guide frame (52) to support the guide magnet (53) to prevent the guide magnet (53) from contacting the positioning magnets.

8. An integrated shaker oscillation method for the integrated shaker according to any one of claims 1-7, characterized in that, It includes the following steps: Determine the oscillation frequency of the enzyme biological cells to be oscillated, and place the container inside the clamping member (3). The oscillation unit (5) drives one of the oscillating plates (21) to oscillate, and the oscillating plate (21) drives other oscillating plates (21) to oscillate synchronously through the connecting unit (6). Adjust the oscillation frequency of each oscillation plate (21) through a differential mechanism; During the oscillation process, the acquisition device (7) acquires the image of the liquid level in the container during the oscillation process, and analyzes the image of the liquid level in the container to determine the fluctuation of the liquid level during the oscillation process.

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