Multi-scene inflatable portable cell culture device

Abstract

The invention discloses a multi-scene inflatable portable cell culture device, and relates to the technical field of biological cell culture.The device comprises a culture cabin, a system cabin and an air cabin, the culture cabin is of a three-layer structure comprising a wear-resistant layer, an inflatable layer and an antibacterial layer and is connected with the system cabin and the air cabin through a double-layer zipper, and the culture cabin is provided with a transparent window and an openable double-layer bottom plate; and a zipper assembly with an automatic sealing structure is connected between the bottom plate and the culture cabin. A heating plate and a temperature sensor are arranged in the antibacterial layer, the system cabin is provided with a temperature controller, a gas controller and a control panel, and the gas cabin is provided with a gas pump and a gas cylinder, supplies gas to the inflation layer and the culture cabin respectively and is electrically connected with the gas controller. The culture cabin of the device can be folded and stored, is good in portability, can be arranged outside a scientific research instrument in a sleeving manner to construct a culture environment in situ, is adaptive to multiple experiment scenes, can accurately regulate and control temperature and gas parameters of the culture cabin, is good in sealing antibacterial activity and convenient to operate, and solves the problems that a traditional culture box is poor in portability and cannot be matched with the scientific research instrument for use.

Description

Technical Field

[0001] This invention relates to the field of biological cell culture technology, specifically a multi-scenario inflatable portable cell culture device. Background Technology

[0002] With the increasing demand for scientific exploration, many studies require cell culture environments to match scientific instruments. For example, when observing the dynamic processes of cells over a long period of time, it is necessary to construct a cell culture environment on a microscope to maintain the normal growth state of cells; when studying the effects of physical environments such as sound, light, and electromagnetic fields on organisms, it is often necessary to use physical field generating equipment to study the effects of stimulation on cells. For example, the Cheng research group has done a lot of research on magnetic field tumor therapy. They used a rotating magnetic field device with a size of about 30cm*30cm*60cm to study the physiological changes of cells under the action of magnetic force (Theranostics 2017, 7(6), 1735-1748); in order to ensure the normal growth of cells, it is necessary to provide them with corresponding temperature, humidity, gas and other environments. The existing cell culture environment mainly relies on rigid cell culture boxes, which achieve environmental control through metal liner and thick insulation layer. The volume is usually large and the portability is poor. Some lightweight improvement schemes have appeared in the existing technology, such as the portable culture box disclosed in patent CN210247725. The weight is reduced by lightweight rigid materials and modular design, which can achieve portability, but it cannot be used in conjunction with scientific research equipment.

[0003] The current compromise solution in the industry is to move the entire research instrument into a rigid incubator. This method is not only cumbersome and time-consuming, but also imposes strict limitations on the size and weight of the instrument, making it completely unsuitable for heavy or large research instruments. This severely restricts the expansion of cell culture-related experiments and research efficiency. Therefore, we are now providing a multi-scenario inflatable portable cell culture device that eliminates the drawbacks of existing devices. Summary of the Invention

[0004] The purpose of this invention is to provide a multi-scenario inflatable portable cell culture device to solve the problems in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A multi-scenario inflatable portable cell culture device includes a culture chamber, a system chamber, and an air chamber. The system chamber and the air chamber are fixedly connected. The culture chamber is connected to the system chamber and the air chamber by a double-layer zipper. The culture chamber has three layers: the outermost layer is a wear-resistant layer, the middle layer is an inflatable layer, and the innermost layer is an antibacterial layer. The antibacterial layer is equipped with a heating plate for heating and a temperature sensor for temperature detection. The system compartment is equipped with a temperature controller and a gas controller for adjusting the temperature inside the culture chamber. The temperature controller is electrically connected to the heating plate and the temperature sensor. The gas chamber is equipped with an air pump for inflating the air layer and a gas cylinder for filling the culture chamber with culture gas. The air pump is connected to the air layer via a detachable pipe, and the gas cylinder is connected to the culture chamber via a detachable pipe. The air pump and the gas cylinder are electrically connected to the gas controller. The system compartment is equipped with a control panel.

[0006] Based on the above technical solutions, the present invention also provides the following optional technical solutions: In one alternative: the sidewall of the culture chamber is hinged to a transparent viewing window.

[0007] In one alternative: the bottom of the culture chamber is provided with a double-layered flip-up bottom plate, which is connected to the culture chamber by a zipper assembly.

[0008] In one alternative: the zipper assembly includes a zipper strip, an adhesive block, and a zipper head. The double-layered flip-up bottom plate and the culture chamber are fitted together by the zipper strip. The side wall of the zipper strip is provided with a guide groove. The zipper head is fixedly connected to two symmetrical sliders. The sliders are slidably connected to the inner wall of the guide groove. A partition plate is fixedly connected to the lower surface of the zipper head.

[0009] In one alternative embodiment: a mounting block is fixedly connected to the lower surface of the culture chamber; the mounting block is rotatably fitted with a positioning block via a bearing seat and a rotating shaft; two symmetrical connecting rods are connected to the side wall of the zipper head via pins; the ends of the two connecting rods away from the zipper head are connected to a fitting block via pins; two rotating block holes are provided on the side walls of both the positioning block and the fitting block; rotating blocks are connected to the inner walls of the rotating block holes via pins; a coil spring is provided between the rotating blocks and the inner walls of the rotating block holes; the rotating blocks are slidably connected to the inner walls of the guide groove; and a sealing film mounting component is provided on the upper surface of the zipper head.

[0010] In one alternative: the lower surfaces of the positioning block and the bonding block are both provided with pressure plate grooves, the inner wall of the pressure plate groove is provided with a pressure plate through a pin, and several springs are fixedly connected between the pressure plate and the pressure plate groove.

[0011] In one alternative embodiment: the sealing membrane mounting component includes a membrane tube, a slot, and a baffle. The zipper head has a slot on its upper surface, the membrane tube is placed in the slot, an insert plate is provided at the bottom of the membrane tube, a magnet is embedded in the bottom wall of the slot, the insert plate is made of magnetic material, a snap-on openable cover plate is provided on the side wall of the membrane tube, a rotating shaft is connected to the inner wall of the membrane tube through a bearing, a PE stretch sealing membrane is sleeved on the rotating shaft, and one end of the PE stretch sealing membrane passes through the bonding block and is pressed down and fixed by the positioning block.

[0012] In one alternative: a baffle is fixedly connected to the inner wall of the slot.

[0013] In one alternative: a sealing strip is provided at the connection between the transparent window and the culture chamber.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: The culture chamber of this invention is initially in a non-air-filled, folded state, occupying little space and easily transportable to different scenarios such as laboratories, outdoors, and research sites. After the air layer is inflated, it forms a supporting structure, eliminating the need for an additional rigid frame. This balances portability and structural stability during use, solving the problems of difficult handling and limited adaptability of traditional rigid culture chambers.

[0015] The culture chamber of this invention features a double-layered, flip-open bottom plate. After flipping open, it can be directly fitted onto the outside of various scientific instruments such as microscopes and rotating magnetic field devices. Through magnetic adsorption and sealing with silicone sealing strips, it creates a cell culture environment that meets the requirements for scientific instruments in situ. There is no need to move heavy or large scientific instruments into the incubator, which completely solves the technical pain point of inconvenience in operating instruments placed in the incubator in existing solutions. It is suitable for the needs of various experimental scenarios such as dynamic cell observation and cell research under physical field stimulation.

[0016] The culture chamber of this invention integrates a heating plate and a temperature sensor, which, together with the temperature controller in the system chamber, enables real-time detection and precise adjustment of the temperature within the culture chamber. The gas cylinders in the gas chamber, in conjunction with the gas controller, can precisely control the concentration of culture gas within the culture chamber. Furthermore, the control panel can display key parameters such as temperature and gas concentration in real time, facilitating real-time monitoring and adjustment by operators. This provides a stable and standardized culture environment for cell growth, ensuring the success rate of cell culture and the accuracy of experimental data.

[0017] The culture chamber, system chamber, and air chamber of this invention are connected by a double-layer zipper. A sealing strip is installed at the connection between the transparent window and the culture chamber to reduce gas leakage structurally. On the other hand, the zipper assembly is equipped with an automatic sealing structure. When the zipper head is pulled to close the zipper, the PE stretch sealing film can automatically cover and press onto the surface of the zipper, achieving precise sealing of the zipper gaps. The double sealing design effectively ensures the airtightness of the culture chamber, prevents the leakage of culture gas and the entry of external bacteria, and maintains the stability of the culture environment inside the chamber.

[0018] The sealing film mounting component of the zipper assembly of this invention uses magnetic adsorption to fix the film tube, making it easy to install and remove. Pulling the zipper head can simultaneously close the zipper and automatically seal the PE stretch sealing film, eliminating the need for additional sealing operations and significantly improving the sealing efficiency of the culture chamber. To unseal, simply tear off the PE stretch sealing film; the operation is simple and efficient. At the same time, the culture chamber is equipped with a transparent window, allowing observation of the internal cell culture without opening the device, reducing environmental fluctuations and the risk of contamination caused by opening the device. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of the present invention.

[0020] Figure 2 This is a first-view diagram of the present invention.

[0021] Figure 3 This is a first-view view of the internal structure of the present invention.

[0022] Figure 4 This is a second-view view of the internal structure of the present invention.

[0023] Figure 5 This is a first-view view of the zipper assembly of the present invention.

[0024] Figure 6 This is a second perspective view of the zipper assembly of the present invention.

[0025] Figure 7 This is a partial schematic diagram of the zipper assembly of the present invention.

[0026] Figure 8 This is a schematic diagram of the bonding block structure of the present invention.

[0027] Figure 9 This is a schematic diagram of the slot structure of the present invention.

[0028] Figure 10 This is a first-view view of the membrane tube of the present invention.

[0029] Figure 11 This is a second-view view of the membrane tube of the present invention.

[0030] Figure label annotations: 1. Culture chamber, 2. System chamber, 3. Air chamber, 5. Transparent window, 6. Inflatable layer, 7. Antibacterial layer, 8. Double zipper, 9. Temperature sensor, 10. Double flip-up bottom plate, 11. Air pump, 12. Air cylinder, 13. Zipper strip, 14. Zipper assembly, 15. PE stretch sealing film, 16. Guide groove, 17. Mounting block, 18. Positioning block, 19. Adhesive block, 20. Zipper head, 21. Membrane tube, 22. Pressure plate, 23. Slider, 24. Rotating block, 25. Connecting rod, 26. Divider plate, 27. Insert plate, 28. Slot, 29. Baffle, 30. Heating plate, 31. Rotary drum shaft, 32. Cover plate. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0032] In one embodiment, such as Figures 1-11 As shown, a multi-scenario inflatable portable cell culture device includes a culture chamber 1, a system chamber 2, and an air chamber 3. The system chamber 2 and the air chamber 3 are fixedly connected. The culture chamber 1 is connected to the system chamber 2 and the air chamber 3 by a double-layer zipper 8. The culture chamber 1 has three layers: the outermost layer is a wear-resistant layer, the middle layer is an inflatable layer 6, and the innermost layer is an antibacterial layer 7. The antibacterial layer 7 is equipped with a heating plate 30 for heating and a temperature sensor 9 for temperature detection. The system compartment 2 is equipped with a temperature controller and a gas controller for adjusting the temperature inside the culture chamber 1. The temperature controller is electrically connected to the heating plate 30 and the temperature sensor 9. The air chamber 3 is equipped with an air pump 11 for inflating the air layer 6 and a gas cylinder 12 for filling the culture chamber 1 with culture gas. The air pump 11 is connected to the air layer 6 through a detachable pipe, and the gas cylinder 12 is connected to the culture chamber 1 through a detachable pipe. The air pump 11 and the gas cylinder 12 are electrically connected to the gas controller. The system compartment 2 is equipped with a control panel.

[0033] Initially, the culture chamber 1 is in a non-air-sealed, folded state. When cell culture is required, the operator removes the culture chamber 1 and simultaneously activates the air pump 11 via the controller to supply air to the air layer 6 in the culture chamber 1. Then, the culture chamber 1 is connected to the system chamber 2 and the air chamber 3 using a double-layer zipper 8. The double-layer flip-up bottom plate 10 is then connected to the culture chamber 1 via the zipper assembly 14. After inflation, the operator wipes the antibacterial layer 7 of the culture chamber 1 with alcohol for sterilization. The transparent viewing window 5 on the front of the culture chamber 1 is opened to place the culture rack. The air pump 11 and the heating plate 30 are activated via the temperature controller and the gas controller to control the gas concentration and temperature in the main culture chamber. Cell culture can then begin. After the control panel is electrically connected to the temperature controller, the gas controller, and the temperature sensor 9, the control panel displays the gas concentration and temperature in the culture chamber 1 for the operator's reference. The gas concentration in the culture chamber 1 is controlled via the gas cylinder 12 and its internal electrically controlled gas valve to maintain the gas concentration at the standard level.

[0034] In one embodiment, the side wall of the culture chamber 1 is connected to a transparent viewing window 5 via a hinge.

[0035] In one embodiment, the bottom of the culture chamber 1 is provided with a double-layer flip-up bottom plate 10, and the double-layer flip-up bottom plate 10 is connected to the culture chamber 1 by a zipper assembly 14.

[0036] In one embodiment, the zipper assembly 14 includes a zipper strip 13, an adhesive block 19, and a zipper head 20. The double-layer flip-up bottom plate 10 and the culture chamber 1 are fitted together by the zipper strip 13. The side wall of the zipper strip 13 is provided with a guide groove 16. The zipper head 20 is fixedly connected to two symmetrical sliders 23. The sliders 23 are slidably connected to the inner wall of the guide groove 16. A partition plate 26 is fixedly connected to the lower surface of the zipper head 20.

[0037] During the connection process, firstly, the insert plate 27 of the membrane tube 21 needs to be inserted into the slot 28. Then, one end of the PE stretch sealing film 15 in the membrane tube 21 is passed through the bonding block 19 and fixed below the positioning block 18. When fixing, the two rotating blocks 24 need to be pressed. The rotating blocks 24 rotate around the pin shaft, disengage from the limit of the guide groove 16, flip the positioning block 18, place one end of the PE stretch sealing film 15 under the positioning block 18, and then let the positioning block 18 return to its original position. Release the finger, and the rotating block 24 will reset under the action of the coil spring and be locked in the guide groove 16 again, thus completing the fixing of one end of the PE stretch sealing film 15.

[0038] In one embodiment, a mounting block 17 is fixedly connected to the lower surface of the culture chamber 1. The mounting block 17 is rotatably fitted with a positioning block 18 via a bearing seat and a rotating shaft. Two symmetrical connecting rods 25 are connected to the side wall of the zipper head 20 via pins. The ends of the two connecting rods 25 away from the zipper head 20 are connected to a fitting block 19 via pins. The side walls of the positioning block 18 and the fitting block 19 are each provided with two rotating block holes. The inner walls of the rotating block holes are connected to rotating blocks 24 via pins. A coil spring is provided between the rotating blocks 24 and the inner walls of the rotating block holes. The rotating blocks 24 are slidably connected to the inner wall of the guide groove 16. A sealing film mounting component is provided on the upper surface of the zipper head 20.

[0039] In one embodiment, both the positioning block 18 and the bonding block 19 have pressure plate grooves on their lower surfaces. A pressure plate 22 is rotatably mounted on the inner wall of the pressure plate groove via a pin. Several springs are fixedly connected between the pressure plate 22 and the pressure plate groove. The springs cause the pressure plate 22 to adhere to the PE stretch sealing film 15.

[0040] In one embodiment, the sealing film mounting component includes a film tube 21, a slot 28, and a baffle 29. The upper surface of the zipper head 20 has a slot 28, in which the film tube 21 is placed. The bottom of the film tube 21 is provided with an insert plate 27. A magnet is embedded in the inner bottom wall of the slot 28. The insert plate 27 is made of magnetic material. The side wall of the film tube 21 is provided with a snap-on openable cover plate 32. The inner wall of the film tube 21 is connected to a rotating shaft 31 through a bearing. A PE stretch sealing film 15 is sleeved on the rotating shaft 31. One end of the PE stretch sealing film 15 passes through the bonding block 19 and is pressed down and fixed by the positioning block 18.

[0041] When the PE stretch sealing film 15 needs to pass through the bonding block 19, similarly to the positioning block 18, the rotating blocks 24 on both sides of the bonding block 19 are pressed to rotate, releasing the restriction on the bonding block 19 and flipping the bonding block 19. After the PE stretch sealing film 15 passes through, the bonding block 19 is returned to its original position. The pressure plate 22 on the lower surface of the bonding block 19 presses against the surface of the PE stretch sealing film 15. When the experimenter holds the film tube 21 and pulls the zipper head 20, the zipper strip 13 is pulled up. At the same time, the PE stretch sealing film 15 is unwound in the film tube 21 and is gradually pulled out of the film tube 21, covering the surface of the zipper strip 13. Since the PE stretch sealing film 15 has a certain degree of adhesion, it is sealed and pressed against the surface of the zipper strip 13 with the cooperation of the pressure plate 22, realizing the automatic sealing of the zipper strip 13. When the seal is released, the PE stretch sealing film 15 can be torn off.

[0042] In one embodiment, a baffle 29 is fixedly connected to the inner wall of the slot 28.

[0043] In one embodiment, a sealing strip is provided at the connection between the transparent window 5 and the culture chamber 1.

[0044] The above embodiments disclose a multi-scenario inflatable portable cell culture device, the specific working principle and process of which are as follows: S1: Initially, the culture chamber 1 is in a non-air-shrinkable folded state. When it is necessary to use this device for cell culture, the operator takes out the culture chamber 1 and starts the air pump 11 through the controller to supply air to the air layer 6 in the culture chamber 1. Then, the culture chamber 1 is connected to the system chamber 2 and the air chamber 3 using a double-layer zipper 8. Then, the double-layer flip-up bottom plate 10 is connected to the culture chamber 1 through the zipper assembly 14. During the connection process, firstly, the insert plate 27 of the membrane tube 21 needs to be inserted into the slot 28. Then, one end of the PE stretch sealing film 15 in the membrane tube 21 is passed through the bonding block 19 and fixed below the positioning block 18. When fixing, the two rotating blocks 24 need to be pressed. The rotating blocks 24 rotate around the pin shaft, disengage from the limit of the guide groove 16, flip the positioning block 18, place one end of the PE stretch sealing film 15 under the positioning block 18, and then let the positioning block 18 return to its original position. Release the finger, and the rotating block 24 will reset under the action of the coil spring and be locked in the guide groove 16 again, thus completing the fixing of one end of the PE stretch sealing film 15. When the PE stretch sealing film 15 needs to pass through the bonding block 19, similar to the positioning block 18, the rotating blocks 24 on both sides of the bonding block 19 are pressed to rotate, releasing the limit on the bonding block 19 and flipping the bonding block 19. After the PE stretch sealing film 15 passes through, the bonding block 19 is restored to its original position. The pressure plate 22 on the lower surface of the bonding block 19 presses against the surface of the PE stretch sealing film 15. When the experimenter holds the film tube 21 and pulls the zipper head 20, the zipper strip 13 is pulled up. At the same time, the PE stretch sealing film 15 is unwound in the film tube 21 and is gradually pulled out of the film tube 21, covering the surface of the zipper strip 13. Since the PE stretch sealing film 15 has a certain degree of adhesion, it is sealed and pressed against the surface of the zipper strip 13 with the cooperation of the pressure plate 22, realizing the automatic sealing of the zipper strip 13. When the seal is released, the PE stretch sealing film 15 can be torn off. S2: After inflation, the operator wipes the antibacterial layer 7 of the culture chamber 1 with alcohol for sterilization, and then opens the transparent window 5 on the front of the culture chamber 1 to place the culture rack. S3: The gas pump 11 and heating plate 30 are started by the temperature controller and gas controller to control the gas concentration and temperature in the main culture chamber. Then the cell culture operation can begin. After the control panel is electrically connected to the temperature controller, gas controller and temperature sensor 9, the control panel will display the gas concentration and temperature in the culture chamber 1 for the operator's understanding. The gas concentration in the culture chamber 1 is controlled by the gas cylinder 12 and its internal electronically controlled gas valve to keep the gas concentration at the standard concentration.

[0045] Open the double-layered bottom plate 10, attach a magnet to the bottom of the culture chamber 1, then put the culture chamber 1 on the microscope. The magnet at the bottom of the culture chamber 1 is attracted to the microscope stage, and a silicone sealing strip is attached to the gap to seal it. Then start the gas cylinder 12 to fill the culture chamber 1 with gas, and use lithium batteries to power the components in the system chamber 2 and the gas chamber 3. After the parameters stabilize, open the transparent window 5 and place the cell culture dish in it. Then close the transparent window 5 and observe the cell morphology through a microscope while simultaneously recording the culture environment parameters.

[0046] The scientific research instruments can be portablely placed in the culture chamber 1, creating a biological culture environment for the scientific research instruments in situ.

[0047] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A multi-scenario inflatable portable cell culture device, characterized in that, It includes a culture chamber (1), a system chamber (2), and an air chamber (3). The system chamber (2) and the air chamber (3) are fixedly connected. The culture chamber (1) is connected to the system chamber (2) and the air chamber (3) by a double-layer zipper (8). The culture chamber (1) has three layers: the outermost layer is a wear-resistant layer, the middle layer is an air-filled layer (6), and the innermost layer is an antibacterial layer (7). The antibacterial layer (7) is provided with a heating plate (30) for heating and a temperature sensor (9) for temperature detection. The system compartment (2) is provided with a temperature controller and a gas controller for adjusting the temperature in the culture chamber (1). The temperature controller is electrically connected to the heating plate (30) and the temperature sensor (9). The air chamber (3) is provided with an air pump (11) for inflating the air layer (6) and a gas cylinder (12) for filling the culture chamber (1) with culture gas. The air pump (11) is connected to the air layer (6) through a detachable pipe. The gas cylinder (12) is connected to the culture chamber (1) through a detachable pipe. The air pump (11) and the gas cylinder (12) are electrically connected to the gas controller. The system compartment (2) is provided with a control panel.

2. The multi-scenario inflatable portable cell culture device according to claim 1, characterized in that, The culture chamber (1) has a transparent viewing window (5) connected to its side wall by a hinge.

3. The multi-scenario inflatable portable cell culture device according to claim 1, characterized in that, The bottom of the culture chamber (1) is provided with a double-layer flip-open bottom plate (10), and the double-layer flip-open bottom plate (10) is connected to the culture chamber (1) by a zipper assembly (14).

4. The multi-scenario inflatable portable cell culture device according to claim 3, characterized in that, The zipper assembly (14) includes a zipper strip (13), a bonding block (19), and a zipper head (20). The double-layer flip-up bottom plate (10) and the culture chamber (1) are fitted together by the zipper strip (13). The side wall of the zipper strip (13) is provided with a guide groove (16). The zipper head (20) is fixedly connected to two symmetrical sliders (23). The sliders (23) are slidably connected to the inner wall of the guide groove (16). The lower surface of the zipper head (20) is fixedly connected to a partition plate (26).

5. A multi-scenario inflatable portable cell culture device according to claim 4, characterized in that, The lower surface of the culture chamber (1) is fixedly connected to an installation block (17). The installation block (17) is rotatably fitted with a positioning block (18) through a bearing seat and a rotating shaft. The side wall of the zipper head (20) is connected to two symmetrical connecting rods (25) through a pin. The end of the two connecting rods (25) away from the zipper head (20) is connected to a fitting block (19) through a pin. The side walls of the positioning block (18) and the fitting block (19) are each provided with two rotating block holes. The inner wall of the rotating block hole is connected to a rotating block (24) through a pin. A coil spring is provided between the rotating block (24) and the inner wall of the rotating block hole. The rotating block (24) is slidably connected to the inner wall of the guide groove (16). A sealing film mounting component is provided on the upper surface of the zipper head (20).

6. A multi-scenario inflatable portable cell culture device according to claim 5, characterized in that, The lower surfaces of the positioning block (18) and the fitting block (19) are provided with pressure plate grooves. The inner wall of the pressure plate groove is provided with a pressure plate (22) through a pin shaft. Several springs are fixedly connected between the pressure plate grooves of the pressure plate (22).

7. A multi-scenario inflatable portable cell culture device according to claim 5, characterized in that, The sealing membrane installation component includes a membrane tube (21), a slot (28), and a baffle (29). The upper surface of the zipper head (20) is provided with a slot (28). The membrane tube (21) is placed in the slot (28). An insert plate (27) is provided at the bottom of the membrane tube (21). A magnet is embedded in the bottom wall of the slot (28). The insert plate (27) is made of magnetic material. A snap-on openable cover plate (32) is provided on the side wall of the membrane tube (21). A rotating shaft (31) is connected to the inner wall of the membrane tube (21) through a bearing. A PE stretch sealing membrane (15) is sleeved on the rotating shaft (31). One end of the PE stretch sealing membrane (15) passes through the bonding block (19) and is pressed down and fixed by the positioning block (18).

8. A multi-scenario inflatable portable cell culture device according to claim 7, characterized in that, A baffle (29) is fixedly connected to the inner wall of the slot (28).

9. A multi-scenario inflatable portable cell culture device according to claim 2, characterized in that, A sealing strip is provided at the connection between the transparent window (5) and the culture chamber (1).

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