A fluorescent quantitative PCR analyzer

Abstract

The present invention relates to the technical field of fluorescence quantitative PCR analyzers, and discloses a fluorescence quantitative PCR analyzer. The PCR analyzer body comprises a shell, a sample storage component, a mixing component, a locking component, an input component and a discharge component, the sample storage component is fixedly connected to the inner side of the shell, the mixing component is installed on the sample storage component, the locking component is installed on the mixing component, and the input component is installed on the sample storage component. Through the sample storage component, the mixing component, the locking component and the input component, the swing of the storage tube drives the diverter seat to swing synchronously, so as to mix the materials in the storage tube. The PCR analyzer body extracts the materials in the material hole of the diverter seat into the reaction chamber through the diverter tube through the microfluidic technology to react, thereby improving the mixing effect of the device, making the yield and quality of the amplification product more stable and reliable, improving the accuracy of the detection result, and facilitating the cleaning operation, reducing the influence of the residue on the detection operation.

Description

Technical Field

[0001] The invention relates to the technical field of fluorescence quantitative PCR analyzers, in particular to a fluorescence quantitative PCR analyzer. Background Art

[0002] Fluorescence quantitative PCR technology has become an indispensable tool in modern molecular biology research and clinical diagnosis due to its advantages of high sensitivity, high specificity and accurate quantitative analysis. However, existing fluorescence quantitative PCR analyzers have many shortcomings.

[0003] In the sample processing stage, the operation of many analyzers is still relatively cumbersome, requiring manual operations such as sample aspiration, transfer and mixing. This is not only inefficient, but also causes uneven mixing of samples and reagents. The sample storage part of the equipment is not convenient to clean, and experimental residues can easily affect the next experiment.

[0004] In order to solve the above problems, this application proposes a fluorescence quantitative PCR analyzer. Summary of the invention

[0005] The purpose of the present invention is to provide a fluorescent quantitative PCR analyzer to solve the problems in the prior art proposed in the above background technology that samples and reagents are not mixed evenly, the sample storage part of the equipment is inconvenient to clean, and experimental residues are likely to affect the next experiment.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a fluorescent quantitative PCR analyzer, wherein the PCR analyzer body comprises a shell, a sample storage component, a mixing component, a locking component, an input component and a discharge component, the sample storage component is fixedly connected to the inner side of the shell, the mixing component is installed on the sample storage component, the locking component is installed on the mixing component, the input component is installed on the sample storage component, and the discharge component is installed on the bottom end of the sample storage component.

[0007] Furthermore, the sample storage assembly includes a material storage tube, an insertion tube, a sealing gasket, a diverter seat, a diverter tube and a microfluidic valve, the top end of the material storage tube is fixedly connected to the insertion tube, the bottom end of the insertion tube is fixedly connected to the sealing gasket, the diverter seat is slidably installed on the outer side of the bottom end of the insertion tube, a material hole is opened on the inner side of the diverter seat, the diverter tube is fixedly connected to the right side of the inner wall of the material hole, and the microfluidic valve is installed on the diverter tube.

[0008] Furthermore, a mounting groove is fixedly connected to the top of the diverter seat, the insertion tube is slidably connected to the inner side wall of the mounting groove, and the bottom end of the insertion tube is in contact with the inner side wall of the mounting groove.

[0009] Furthermore, the mixing assembly includes a pipe sleeve, a bracket, a base, a rotating frame, a sliding rod, a slider, a rotating rod and a motor; the pipe sleeve is slidably connected to the outer side of the storage tube, the right end of the pipe sleeve is fixedly connected to the bracket, the bottom end of the bracket is fixedly connected to the base, the top of the base is fixedly connected to the diverter seat, the left end of the pipe sleeve is fixedly connected to the rotating frame, the top of the rotating frame is fixedly connected to the sliding rod, the slider is slidably connected to the outer side of the sliding rod, the right end of the slider is rotatably connected to the rotating rod via a rotating shaft, and the right end of the rotating rod is fixedly connected to the output end of the motor.

[0010] Furthermore, the mixing assembly also includes a protective shell, the left end of the shell is fixedly connected to the protective shell, the sliding rod is arranged on the inner side of the protective shell, and the left end of the rotating frame is rotatably connected to the protective shell through a rotating shaft.

[0011] Furthermore, the locking assembly includes a slide seat, a wedge block, a push block, an electric telescopic rod and a connecting rod. The right end of the bracket is fixedly connected to the slide seat, the wedge block is slidably connected to the inner side of the slide seat, the insertion tube is fixedly connected to the outer side of the insertion tube, the top of the slide seat is fixedly connected to the electric telescopic rod, the right end of the electric telescopic rod is fixedly connected to the connecting rod, and the other end of the connecting rod is fixedly connected to the wedge block.

[0012] Furthermore, the connecting rod passes through the surface of the sliding seat, the outer side of the connecting rod is slidably connected to the sliding seat, a limiting groove is opened on the inner side of the wedge block, the outer side of the bracket is slidably connected to the limiting groove, the outer side of the insertion tube is slidably connected to the limiting groove, the left bottom end of the wedge block is inclined, the movement trajectory of the wedge block is straight, and the push block is arranged on the movement trajectory of the wedge block.

[0013] Furthermore, the input component includes a sealing cover, a fixed tube, a sliding tube, a water inlet pipe, a feed pipe and a solenoid valve; the sealing cover is frictionally connected to the top of the storage tube, the sealing cover is fixedly connected to the top of the fixed tube, the sliding tube is fixedly connected to the top of the fixed tube, the other end of the sliding tube is fixedly connected to the water inlet pipe and the feed pipe, and the solenoid valve is installed on both the water inlet pipe and the feed pipe.

[0014] Furthermore, the input component also includes a flip cover, a maintenance groove is opened on the left side of the top of the shell, the left end of the inner wall of the maintenance groove is rotatably connected to the flip cover via a rotating shaft, the outer side of the sliding tube passes through the surface of the flip cover, and the outer side of the sliding tube is slidably connected to the flip cover.

[0015] Furthermore, the discharge assembly includes a discharge pipe and a discharge valve. The discharge pipe is fixedly connected to the left side of the material hole of the diverter seat. The discharge valve is installed on the discharge pipe. The discharge pipe passes through the left end surface of the shell.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The present invention provides a sample storage component, a mixing component, a locking component and an input component. The swing of the storage tube drives the diverter seat to swing synchronously, thereby mixing the materials in the storage tube. The PCR analyzer body extracts the materials in the material holes of the diverter seat into the reaction chamber through the diverter tube by microfluidic technology to react, thereby improving the mixing effect of the device, making the output and quality of the amplification product more stable and reliable, improving the accuracy of the detection result, facilitating the cleaning operation, and reducing the influence of residues on the detection operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the overall structure of a fluorescent quantitative PCR analyzer of the present invention;

[0019] Figure 2 This is a schematic diagram of the installation structure of a sample storage component of a fluorescent quantitative PCR analyzer of the present invention;

[0020] Figure 3 It is a schematic diagram of the installation structure of a mixing component of a fluorescence quantitative PCR analyzer of the present invention;

[0021] Figure 4 It is a schematic diagram of the installation structure of a locking component of a fluorescence quantitative PCR analyzer of the present invention;

[0022] Figure 5 A top view of the installation structure of a wedge block of a fluorescence quantitative PCR analyzer according to the present invention;

[0023] Figure 6 This is a schematic diagram of the installation structure of an input component of a fluorescence quantitative PCR analyzer of the present invention;

[0024] Figure 7 This is a schematic diagram of the installation structure of a discharge component of a fluorescent quantitative PCR analyzer of the present invention;

[0025] In the figure:

[0026] 1. PCR analyzer body;

[0027] 11. Shell;

[0028] 12. Sample storage assembly; 121. Storage tube; 122. Insertion tube; 123. Sealing pad; 124. Diverter seat; 125. Diverter tube; 126. Microflow valve;

[0029] 13. Mixing assembly; 131. Pipe sleeve; 132. Bracket; 133. Base; 134. Rotating frame; 135. Sliding rod; 136. Sliding block; 137. Rotating rod; 138. Motor; 139. Protective shell;

[0030] 14. Locking assembly; 141. Sliding seat; 142. Wedge block; 143. Push block; 144. Electric telescopic rod; 145. Connecting rod;

[0031] 15. Input assembly; 151. Cover; 152. Fixed pipe; 153. Sliding pipe; 154. Flip cover; 155. Water inlet pipe; 156. Feed pipe; 157. Solenoid valve;

[0032] 16. discharge assembly; 161. discharge pipe; 162. discharge valve. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0034] The standard parts used in this application can all be purchased from the market, and special-shaped parts can be customized according to the instructions and drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, and bonding in the existing technology, and the components used for circuit connection are all conventional models in the existing technology.

[0035] At the same time, in order to clearly express the connection relationship and working principle between the components and highlight the key points, the drawings in the specification are organized and drawn in the form of simple diagrams. One simple diagram can correspond to a variety of materials and actual external structural shapes.

[0036] See also Figure 1-Figure 7The present invention provides a technical solution: a fluorescent quantitative PCR analyzer, the PCR analyzer body 1 includes a shell 11, a sample storage component 12, a mixing component 13, a locking component 14, an input component 15 and a discharge component 16, the sample storage component 12 is fixedly connected to the inner side of the shell 11, and the mixing component 13 is installed on the sample storage component 12. The locking component 14 is installed on the mixing component 13, the input component 15 is installed on the sample storage component 12, and the discharge component 16 is installed at the bottom of the sample storage component 12, and a micro pump is installed on the feed pipe 156, and the side of the feed pipe 156 away from the PCR analyzer body 1 is connected to the sample bin, and then the micro pump is operated to extract the material in the sample bin, the PCR analyzer body 1 controls the electromagnetic valve 157 on the feed pipe 156 to open, and the material enters the slide pipe 153 through the feed pipe 156, and the material enters the shell 11 through the fixed pipe 152, and the cover 151 is sleeved at the top opening of the storage pipe 121, which is used for connecting the fixed pipe 152 and the storage pipe 121, and in order to avoid mixing components During the operation of the material storage tube 121, the material is added to the inner side of the material storage tube 121, and then the motor 138 is operated to drive the rotating rod 137 to rotate, and the rotating rod 137 drives the slider 136 to slide on the sliding rod 135, thereby driving the sliding rod 135 to swing back and forth around the axis of the rotating frame 134, and the bottom end of the sliding rod 135 drives the rotating frame 134 to rotate back and forth, and the rotating frame 134 drives the pipe sleeve 131 to rotate back and forth, thereby driving the material storage tube 121 to swing back and forth, and the bracket 132 is used to connect the material storage tube 121 to the material storage tube 121. The pipe sleeve 131 and the base 133, the base 133 supports the diverter seat 124, the storage tube 121 swings to drive the diverter seat 124 to swing synchronously, so as to mix the materials in the storage tube 121, and then the PCR analyzer body 1 operates the microfluidic valve 126 to open, and the materials in the storage tube 121 enter the material hole of the diverter seat 124 through the insertion tube 122, and the PCR analyzer body 1 extracts the materials in the material hole of the diverter seat 124 to the reaction chamber through the diverter tube 125 by microfluidic technology for reaction.

[0037] It should be noted that when using the parabolic reflector, the reflector should be accurately mounted on the optical path bracket, and the angle and position of the reflector should be adjusted by a precise adjustment device so that its focus is accurately aligned with the sample position to ensure that the fluorescence signal is collected to the greatest extent. The installation accuracy of the reflector is repeatedly calibrated using a high-precision optical alignment instrument to ensure that the optical axis of the reflector is accurately aligned with the input end of the fiber coupler.

[0038] It should be noted that when installing the fiber coupler, a special fixing device is used to ensure its stability and avoid displacement during the operation of the instrument. The coupling efficiency of the fiber coupler is tested and optimized by an optical power meter, and the parameters of the coupler are adjusted to maintain high coupling efficiency and stability under different wavelengths of fluorescence signals.

[0039] It should be noted that: for the spectroscopic device, according to the requirements of different fluorescence wavelengths, select appropriate spectroscopic elements such as gratings or filters for installation and debugging, and calibrate the spectroscopic device using a standard fluorescent light source to ensure that each channel can accurately separate and transmit the fluorescence signal of the corresponding wavelength to avoid crosstalk between channels.

[0040] It should be noted that when setting parameters through the intelligent PID controller, a preliminary theoretical calculation is first performed to determine the approximate PID parameter range. Then, through actual temperature control experiments, the temperature change curves under different temperature set points and reaction conditions are monitored and analyzed, and the PID parameters are repeatedly adjusted and optimized according to the experimental results until the temperature control system can quickly and stably control the reaction chamber temperature within the range of ±0.1°C of the set value under various working conditions, meeting the strict requirements of PCR reaction on temperature accuracy.

[0041] The sample storage component 12 includes a storage tube 121, an insertion tube 122, a sealing gasket 123, a diverter seat 124, a diverter tube 125 and a micro-flow valve 126. The top of the storage tube 121 is fixedly connected to the insertion tube 122, the bottom of the insertion tube 122 is fixedly connected to the sealing gasket 123, the diverter seat 124 is slidably installed on the outer side of the bottom end of the insertion tube 122, a material hole is opened on the inner side of the diverter seat 124, the right side of the inner wall of the material hole is fixedly connected to the diverter tube 125, and the micro-flow valve 126 is installed on the diverter tube 125.

[0042] The top of the shunt seat 124 is fixedly connected with a mounting groove, the inner wall of the mounting groove is slidably connected with an insertion tube 122, and the bottom end of the insertion tube 122 is in contact with the inner wall of the mounting groove.

[0043] The mixing assembly 13 includes a sleeve 131, a bracket 132, a base 133, a rotating frame 134, a sliding rod 135, a slider 136, a rotating rod 137 and a motor 138. The sleeve 131 is slidably connected to the outer side of the storage tube 121, the right end of the sleeve 131 is fixedly connected to the bracket 132, the bottom end of the bracket 132 is fixedly connected to the base 133, the top of the base 133 is fixedly connected to the diverter seat 124, the left end of the sleeve 131 is fixedly connected to the rotating frame 134, the top of the rotating frame 134 is fixedly connected to the sliding rod 135, the outer side of the sliding rod 135 is slidably connected to the slider 136, the right end of the slider 136 is rotatably connected to the rotating rod 137 through a rotating shaft, and the right end of the rotating rod 137 is fixedly connected to the output end of the motor 138.

[0044] The mixing assembly 13 further includes a protective shell 139 . The left end of the housing 11 is fixedly connected with the protective shell 139 . The slide bar 135 is arranged inside the protective shell 139 . The left end of the rotating frame 134 is rotatably connected with the protective shell 139 via a rotating shaft.

[0045] The locking assembly 14 includes a slide 141, a wedge block 142, a push block 143, an electric telescopic rod 144 and a connecting rod 145. The right end of the bracket 132 is fixedly connected to the slide 141, the wedge block 142 is slidably connected to the inner side of the slide 141, the insertion tube 122 is fixedly connected to the outer side of the insertion tube 122, the top of the slide 141 is fixedly connected to the electric telescopic rod 144, the right end of the electric telescopic rod 144 is fixedly connected to the connecting rod 145, and the other end of the connecting rod 145 is fixedly connected to the wedge block 142.

[0046] The connecting rod 145 penetrates the surface of the slide 141, and the outer side of the connecting rod 145 is slidably connected to the slide 141. A limiting groove is provided on the inner side of the wedge block 142, and the outer side of the bracket 132 is slidably connected to the limiting groove. The outer side of the insertion tube 122 is slidably connected to the limiting groove. The left bottom end of the wedge block 142 is inclined, and the movement trajectory of the wedge block 142 is linear. The push block 143 is set on the movement trajectory of the wedge block 142. The electric telescopic rod 144 drives the connecting rod 145 to move rightward, and the connecting rod 145 drives the wedge block 142 to move rightward and separate from the push block 143, and then the flip cover 154 is rotated to open the shell 11, and then the storage tube 121 is pulled out so that the insertion tube 1 22 is separated from the diverter seat 124, and the cover 151 and the storage tube 121 are pulled to separate the storage tube 121 from the cover 151, and the storage tube 121 is cleaned by high-temperature disinfection and other cleaning operations. After use, the storage tube 121 is reset so that the insertion tube 122 is inserted into the diverter seat 124, and the sealing gasket 123 is used for the sealing connection between the insertion tube 122 and the diverter seat 124. Then the electric telescopic rod 144 is reset and retracted to drive the connecting rod 145 to move to the left. The connecting rod 145 drives the wedge block 142 to the left and presses the push block 143 downward to complete the limiting fixation of the storage tube 121, and the cover 151 is opened at the top of the storage tube 121, and the flip cover 154 is reset.

[0047] The input assembly 15 includes a cover 151, a fixed tube 152, a slide tube 153, a water inlet pipe 155, a feed pipe 156 and a solenoid valve 157. The top of the storage tube 121 is frictionally connected with the cover 151, the top of the cover 151 is fixedly connected with the fixed tube 152, the top of the fixed tube 152 is fixedly connected with the slide tube 153, the other end of the slide tube 153 is fixedly connected with the water inlet pipe 155 and the feed pipe 156, and the solenoid valve 157 is installed on the water inlet pipe 155 and the feed pipe 156. The input assembly 15 also includes a flip cover 154. A maintenance groove is opened on the left side of the top of the shell 11. The left end of the inner wall of the maintenance groove is rotatably connected to the flip cover 154 through a rotating shaft. The outer side of the slide tube 153 passes through the surface of the flip cover 154, and the outer side of the slide tube 153 is connected to the flip cover 1 54 sliding connection, the PCR analyzer body 1 controls the solenoid valve 157 on the feed tube 156 to open, the material enters the slide tube 153 through the feed tube 156, and the material enters the shell 11 through the fixed tube 152, and the cover 151 is sleeved on the top opening of the storage tube 121, which is used to connect the fixed tube 152 and the storage tube 121, and avoid the storage tube 121 from leaking when the mixing component 13 is running, thereby improving the stability of the storage tube 121. When cleaning the sample storage component 12, the water inlet pipe 155 is connected to the pure water pipe, the solenoid valve 157 is opened, and pure water is poured into the storage tube 121, and then the operation of the mixing component 13 drives the sample storage component 12 to shake, and then the discharge valve 162 is opened to discharge the water through the discharge pipe 161.

[0048] The discharge component 16 includes a discharge pipe 161 and a discharge valve 162. The discharge pipe 161 is fixedly connected to the left side of the material hole of the diverter seat 124. The discharge valve 162 is installed on the discharge pipe 161. The discharge pipe 161 passes through the left end surface of the shell 11. When cleaning the sample storage component 12, the water inlet pipe 155 is connected to the pure water pipe, the solenoid valve 157 is opened, and the pure water is poured into the storage pipe 121. Then, the mixing component 13 is operated to drive the sample storage component 12 to shake, and then the discharge valve 162 is opened to discharge the water through the discharge pipe 161.

[0049] Working principle:

[0050] When the device is in use, a micro pump is installed on the feed pipe 156, and the side of the feed pipe 156 away from the PCR analyzer body 1 is connected to the sample bin, and then the micro pump is operated to extract the material in the sample bin, and the PCR analyzer body 1 controls the electromagnetic valve 157 on the feed pipe 156 to open, and the material enters the slide pipe 153 through the feed pipe 156, and the material enters the housing 11 through the fixed pipe 152. The cover 151 is sleeved at the top opening of the storage tube 121, and is used for connecting the fixed pipe 152 and the storage tube 121, and avoids leakage of the storage tube 121 when the mixing assembly 13 is running, thereby improving the stability of the storage tube 121. After the material is added to the inner side of the storage tube 121, the motor 138 is operated to drive the rotating rod 137 to rotate, and the rotating rod 137 drives the slider 136 to slide on the slide bar 135, thereby driving the slide bar 135 to swing back and forth around the axis of the rotating frame 134, and the bottom end of the slide bar 135 drives the rotating frame 134 to rotate back and forth, and the rotating frame 134 drives the pipe sleeve 131 to rotate back and forth, thereby driving the storage tube 121 to swing back and forth, and the bracket 132 is used to connect the pipe sleeve 131 and the base 133, and the base 133 supports the diverter seat 124. The swing of the storage tube 121 drives the diverter seat 124 to swing synchronously, thereby mixing the materials in the storage tube 121;

[0051] Then add reagents, connect the water inlet pipe 155 to the reagent bin, open the solenoid valve 157, and pour the reagents into the storage tube 121. Then the PCR analyzer body 1 runs the microfluidic valve 126 to open, and the material in the storage tube 121 enters the material hole of the shunt seat 124 through the insertion tube 122. The PCR analyzer body 1 extracts the material in the material hole of the shunt seat 124 to the reaction bin through the shunt tube 125 by microfluidic technology for reaction, so as to facilitate the mixing operation and make the sample and reagent materials fully mixed. When cleaning the sample storage component 12, the water inlet pipe 155 is connected to the pure water pipe, and the solenoid valve 157 is opened. Clean water is poured into the storage tube 121, and then the mixing component 13 is operated to drive the sample storage component 12 to shake, and then the discharge valve 162 is opened to discharge the water. The electric telescopic rod 144 can also be operated to drive the connecting rod 145 to move to the right, and the connecting rod 145 drives the wedge block 142 to move to the right and separate from the push block 143, and then the flip cover 154 is rotated to open the shell 11, and the storage tube 121 is pulled out to separate the insertion tube 122 from the diverter seat 124, and the cover 151 and the storage tube 121 are pulled to separate the storage tube 121 from the cover 151, and the storage tube 121 is cleaned by high-temperature disinfection and other cleaning operations.

Claims

1. A fluorescence quantitative PCR analyzer, characterized in that: The PCR analyzer body (1) comprises a shell (11), a sample storage component (12), a mixing component (13), a locking component (14), an input component (15) and a discharge component (16); the sample storage component (12) is fixedly connected to the inner side of the shell (11); the mixing component (13) is installed on the sample storage component (12); the locking component (14) is installed on the mixing component (13); the input component (15) is installed on the sample storage component (12); and the discharge component (16) is installed at the bottom end of the sample storage component (12).

2. A fluorescence quantitative PCR analyzer according to claim 1, characterized in that: The sample storage component (12) comprises a material storage tube (121), an insertion tube (122), a sealing gasket (123), a diverter seat (124), a diverter tube (125) and a micro-flow valve (126); the top end of the material storage tube (121) is fixedly connected to the insertion tube (122); the bottom end of the insertion tube (122) is fixedly connected to the sealing gasket (123); the diverter seat (124) is slidably mounted on the outer side of the bottom end of the insertion tube (122); a material hole is opened on the inner side of the diverter seat (124); the diverter tube (125) is fixedly connected to the right side of the inner wall of the material hole; and the micro-flow valve (126) is mounted on the diverter tube (125).

3. A fluorescence quantitative PCR analyzer according to claim 2, characterized in that: The top of the diverter seat (124) is fixedly connected to a mounting groove, the inner side wall of the mounting groove is slidably connected to the insertion tube (122), and the bottom end of the insertion tube (122) is in contact with the inner side wall of the mounting groove.

4. A fluorescence quantitative PCR analyzer according to claim 3, characterized in that: The mixing assembly (13) comprises a pipe sleeve (131), a bracket (132), a base (133), a rotating frame (134), a sliding rod (135), a sliding block (136), a rotating rod (137) and a motor (138); the outer side of the storage tube (121) is slidably connected to the pipe sleeve (131); the right end of the pipe sleeve (131) is fixedly connected to the bracket (132); the bottom end of the bracket (132) is fixedly connected to the base (133); the base ( The top of the pipe sleeve (133) is fixedly connected to the diverter seat (124), the left end of the pipe sleeve (131) is fixedly connected to the rotating frame (134), the top of the rotating frame (134) is fixedly connected to the sliding rod (135), the outer side of the sliding rod (135) is slidably connected to the slider (136), the right end of the slider (136) is rotatably connected to the rotating rod (137) via a rotating shaft, and the right end of the rotating rod (137) is fixedly connected to the output end of the motor (138).

5. A fluorescence quantitative PCR analyzer according to claim 4, characterized in that: The mixing assembly (13) further comprises a protective shell (139), the left end of the housing (11) being fixedly connected to the protective shell (139), the sliding rod (135) being arranged inside the protective shell (139), and the left end of the rotating frame (134) being rotatably connected to the protective shell (139) via a rotating shaft.

6. A fluorescence quantitative PCR analyzer according to claim 4, characterized in that: The locking assembly (14) comprises a sliding seat (141), a wedge block (142), a push block (143), an electric telescopic rod (144) and a connecting rod (145); the right end of the bracket (132) is fixedly connected to the sliding seat (141); the inner side of the sliding seat (141) is slidably connected to the wedge block (142); the outer side of the insertion tube (122) is fixedly connected to the insertion tube (122); the top end of the sliding seat (141) is fixedly connected to the electric telescopic rod (144); the right end of the electric telescopic rod (144) is fixedly connected to the connecting rod (145); the other end of the connecting rod (145) is fixedly connected to the wedge block (142).

7. A fluorescence quantitative PCR analyzer according to claim 6, characterized in that: The connecting rod (145) passes through the surface of the sliding seat (141), the outer side of the connecting rod (145) is slidably connected to the sliding seat (141), the inner side of the wedge block (142) is provided with a limiting groove, the outer side of the bracket (132) is slidably connected to the limiting groove, the outer side of the insertion tube (122) is slidably connected to the limiting groove, the left bottom end of the wedge block (142) is inclined, the movement trajectory of the wedge block (142) is linear, and the push block (143) is arranged on the movement trajectory of the wedge block (142).

8. A fluorescence quantitative PCR analyzer according to claim 7, characterized in that: The input component (15) comprises a sealing cover (151), a fixed tube (152), a sliding tube (153), a water inlet tube (155), a feed tube (156) and a solenoid valve (157); the top end of the storage tube (121) is frictionally connected to the sealing cover (151); the top end of the sealing cover (151) is fixedly connected to the fixed tube (152); the top end of the fixed tube (152) is fixedly connected to the sliding tube (153); the other end of the sliding tube (153) is fixedly connected to the water inlet tube (155) and the feed tube (156); and the solenoid valve (157) is installed on both the water inlet tube (155) and the feed tube (156).

9. A fluorescent quantitative PCR analyzer according to claim 8, characterized in that: The input component (15) further comprises a flip cover (154), a maintenance slot is provided on the left side of the top end of the housing (11), the left end of the inner wall of the maintenance slot is rotatably connected to the flip cover (154) via a rotating shaft, the outer side of the slide tube (153) passes through the surface of the flip cover (154), and the outer side of the slide tube (153) is slidably connected to the flip cover (154).

10. A fluorescence quantitative PCR analyzer according to claim 9, characterized in that: The discharge assembly (16) comprises a discharge pipe (161) and a discharge valve (162); the discharge pipe (161) is fixedly connected to the left side of the material hole of the diverter seat (124); the discharge valve (162) is installed on the discharge pipe (161); and the discharge pipe (161) passes through the left end surface of the shell (11).

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