A method for detecting light intensity of micro-volume liquid

Abstract

The present invention discloses a method for detecting light intensity of micro-volume liquid, comprising the steps of: setting a detection light path volume V1; obtaining the volume V1 of all liquids; 总 ; The number of tests m required to obtain the test sample; respectively according to 2 / n*V 总 、3 / n*V 总 , 4 / n*V 总 ……(m+1) / n*V 总 The test sample is discharged with a liquid output, and a quantitative sample is passed through the detection module in turn, and the detection light intensity A of the sample under each liquid output is recorded respectively; when the number of detections m≤2, the average value of the m recorded detection light intensities A is calculated; when the number of detections m>2, the absolute value of the difference between two adjacent detection light intensities A is calculated, and the detection light intensity with the maximum absolute value of the difference between the two adjacent detection light intensities A is eliminated, and the remaining detection light intensities A are averaged. This method does not require any additional cost and improves the accuracy of the method detection, and is simple to operate, and can be applied to optical detection of all trace volumes of liquids.

Description

Technical Field

[0001] The invention relates to a water quality online monitoring technology, and in particular to a light intensity detection method for a micro-volume liquid. Background Art

[0002] Micro-system metering module technology is applied to water quality wet chemical analysis instruments. It has the advantages of small integrated volume, fast response speed, high analysis efficiency, good safety performance, extremely low reagent consumption and waste liquid volume, and low maintenance cost. However, due to the small channel size of the detection module, bubbles are easily generated and have a great impact on the accuracy of the test results. Summary of the invention

[0003] In order to solve the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a method for detecting light intensity of micro-volume liquids, which does not increase any cost while improving the accuracy of method detection, and is simple to operate and can be applied to optical detection of all micro-volume liquids.

[0004] The technical solution adopted by the present invention to solve the technical problem is: a method for detecting light intensity of a micro-volume liquid, comprising the following steps: Obtain and set the detection optical path volume V1 of the detection module; Measure and obtain the volume V of all liquids entering the reaction module 总 ; The number of tests required to obtain the test sample is m, where m=(V 总 / V1)-2; According to 2 / n*V 总 、3 / n*V 总 , 4 / n*V 总 ……(m+1) / n*V 总 The test sample is discharged at a liquid output volume, so that a certain amount of sample passes through the detection module in sequence, and the detection light intensity A of the sample at each liquid output volume is recorded respectively, where n=m+2; When the number of detections obtained is m≤2, the average value of the detected light intensities A of the m records is calculated; When the acquired detection times m>2, the absolute value of the difference between two adjacent detection light intensities A is calculated, and the detection light intensity with the maximum absolute value of the difference between the two adjacent detection light intensities A is eliminated, and the remaining detection light intensities A are averaged.

[0005] Optionally, when obtaining the number of detections m, first obtain the value of n, where n = V 总 / V1, and n is rounded to an integer when obtained; The value of the detection number m is n-2, and is an integer.

[0006] Optionally, the detection module includes a detection channel, the detection channel is provided with an effective optical path segment, and a light source and a photoelectric receiver are respectively provided at both ends of the effective optical path segment; The volume of the effective optical path section of the detection channel is V1.

[0007] Optionally, when discharging the test sample, the power source used is a peristaltic pump or a plunger pump; When the power source is a peristaltic pump, the liquid output of the peristaltic pump is less than or equal to 5 ml / min; When the power source is a plunger pump, the maximum stroke of the plunger pump is less than or equal to 5 ml.

[0008] By adopting the above technical scheme, the present invention improves the detection method of the entire instrument to address the shortcomings of bubbles being easily generated during optical detection in a micro system and having a significant impact on the accuracy of the test results. This improves the accuracy of the method detection without increasing any cost and is simple to operate. DETAILED DESCRIPTION

[0009] The present application is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the relevant invention, rather than to limit the invention.

[0010] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present application will be described in detail with reference to the embodiments below.

[0011] The invention discloses a light intensity detection method for micro-volume liquid. The light intensity detection method is mainly used to remove the interference of bubbles, thereby improving the accuracy of light intensity detection of micro-volume liquid.

[0012] In one embodiment of the present invention, the method is applied to an online water quality monitoring instrument, which can reduce the amount of sample used and improve the detection accuracy. In the online water quality monitoring instrument, the light intensity detection method is to use the driving force module, metering module, reaction module, detection module and control module in the online water quality monitoring instrument to realize the light intensity detection of the micro volume liquid. The specific implementation steps of the method are: S1. Set the detection optical path volume V1 of the detection module.

[0013] The control panel of the water quality online monitoring instrument is provided with a detection optical path volume setting interface, in which the detection optical path volume V1 of the corresponding detection module is set. According to the different detection optical paths, the detection optical path volume V1 of the corresponding detection module has different ranges, and the value can be set. The detection optical path volume V1 volume range is 10ul~1ml.

[0014] Specifically, the detection module includes a detection channel, which is provided with an effective optical path segment, and a light source and a photoelectric receiver are respectively provided at both ends of the effective optical path segment. The volume of the effective optical path segment of the detection channel is V1, that is, the volume of the detection channel is the detection optical path volume, and the transmittance of the detection channel needs to reach at least 80%.

[0015] S2. Measure and obtain the volume V of all liquids entering the reaction module 总 .

[0016] When the instrument is started, the metering module can measure all the liquids entering the reaction module and transmit the measurement results to the control module. The control module sums the volumes of all the liquids entering the reaction module to obtain the total volume V of all the liquids entering the reaction module. 总 .

[0017] The liquid entering the reaction module includes the sample or standard substance used, all the reagents used for the reaction required for the test, and the pure water entering the reaction module through the flow path for diluting or cleaning the residual liquid. 总 The volume range is 30ul~5ml.

[0018] S3, according to the formula n=V 总 / V1 is used to get n, n is rounded off according to the principle of rounding off, and then m is obtained according to m=n-2, where m is the number of measurements required for light intensity detection.

[0019] S4. When the sample to be tested enters the detection module, the number of tests required is determined according to the calculated m. At the same time, according to the relationship between the stroke and the output of the driving force module of the water quality online monitoring instrument (including but not limited to a power source with a metering function such as a peristaltic pump or a plunger pump), the output is calculated according to the output 2 / n*V. 总 、3 / n*V 总 , 4 / n*V 总 ……(m+1) / n*V 总 (m times), and record the corresponding detection light intensities A1, A2, A3...A respectively. m (m pieces), and the remaining test samples are discharged into the analytical waste liquid.

[0020] S5. If the tested m is ≤2, then take the average value and perform corresponding calculation and display, and record and store it according to the set data storage format.

[0021] If m>2, then obtain │A1-A2│, │A2-A3│, ...│A in sequence m -A1│, then remove the abnormal value whose absolute value of the difference between the two adjacent values ​​is the maximum, and then take the average value of the remaining values ​​for corresponding calculation and display, and record and store according to the set data storage format.

[0022] In the present invention, the driving force module and the metering module are connected through a first three-way solenoid valve. Specifically, the normally open end of the first three-way solenoid valve is connected to the driving force module, and the common channel end is connected to the metering module. The reaction module and the liquid outlet of the metering module are connected through a second three-way solenoid valve, the normally open end of the second three-way solenoid valve is connected to the liquid outlet of the metering module, the normally closed end is connected to the liquid inlet of the detection module, and the common channel end is connected to the bottom of the reaction module for liquid inlet and liquid outlet, the liquid outlet of the detection module is connected to the liquid inlet of a DC motor pump, and the liquid outlet of the DC motor pump is connected to the cleaning waste liquid channel and the analysis waste liquid channel respectively through a third three-way solenoid valve, wherein the common channel end of the third three-way solenoid valve is connected to the liquid outlet of the detection module, the normally open end is connected to the analysis waste liquid channel, and the normally closed end is connected to the cleaning waste liquid channel, for discharging cleaning waste liquid and analysis waste liquid.

[0023] In the present invention, all connecting pipes are made of PTFE pipes, the pipe diameter is 1.2-1.6mm, and the three-way solenoid valve used has a diameter range of 1.2-1.6mm. The reaction module is a reaction pool including a mixed reaction module or a mixed reaction module and a heating constant temperature digestion function. The reaction pool material includes but is not limited to quartz glass, ceramics and other materials. When liquid is introduced, the control module records the liquid metering level and number of times entering the reaction module each time, and calculates the total volume V of all liquids entering the reaction module after the liquid is introduced by the cumulative method. 总 .

[0024] In the present invention, the driving force module can adopt a power source such as a peristaltic pump or a plunger pump or other power sources with a certain proportional relationship between the stroke and the liquid storage volume. When a peristaltic pump is adopted, the flow rate requirement of the peristaltic pump adopted must meet the liquid output of ≤5ml / min. When a plunger pump is adopted, the requirement for the plunger pump is that the maximum stroke must meet ≤5ml.

[0025] The above description is only a preferred embodiment of the present application and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the present application is not limited to the technical solution formed by a specific combination of the above technical features, but should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the inventive concept. For example, the above features are replaced with (but not limited to) technical features with similar functions disclosed in the present application.

[0026] Except for the technical features described in the specification, the remaining technical features are known technologies to those skilled in the art. In order to highlight the innovative features of the present invention, the remaining technical features will not be described here in detail.

Claims

1. A method for detecting light intensity of a micro-volume liquid, characterized in that: The following steps are included: Obtain and set the detection optical path volume V1 of the detection module; Measure and obtain the volume V of all liquids entering the reaction module 总 ; The number of tests required to obtain the test sample is m, where m=(V 总 / V1)-2; According to 2 / n*V 总 、3 / n*V 总 , 4 / n*V 总 ……(m+1) / n*V 总 The test sample is discharged at a liquid output volume, so that a certain amount of sample passes through the detection module in sequence, and the detection light intensity A of the sample at each liquid output volume is recorded respectively, where n=m+2; When the number of detections obtained is m≤2, the average value of the detected light intensities A of the m records is calculated; When the acquired detection times m>2, the absolute value of the difference between two adjacent detection light intensities A is calculated, and the detection light intensity with the maximum absolute value of the difference between the two adjacent detection light intensities A is eliminated, and the remaining detection light intensities A are averaged.

2. The light intensity detection method of micro-volume liquid according to claim 1, characterized in that: When obtaining the number of detections m, first obtain the value of n, where n = V 总 / V1, and n is rounded to an integer when obtained; The value of the detection number m is n-2, and is an integer.

3. The light intensity detection method of micro-volume liquid according to claim 2, characterized in that: The detection module comprises a detection channel, wherein the detection channel is provided with an effective optical path segment, and a light source and a photoelectric receiver are respectively provided at both ends of the effective optical path segment; The volume of the effective optical path section of the detection channel is V1.

4. The light intensity detection method of micro-volume liquid according to claim 3, characterized in that: When discharging the test sample, the power source used is a peristaltic pump or a plunger pump; When the power source is a peristaltic pump, the liquid output of the peristaltic pump is less than or equal to 5 ml / min; When the power source is a plunger pump, the maximum stroke of the plunger pump is less than or equal to 5 ml.