In-situ biological detection system and detection method based on plug-in Raman probe

Abstract

The invention discloses an in-situ biological detection system and detection method based on a plug-in Raman probe. The in-situ biological detection system comprises a Raman detection device and a biological detection cabin, the Raman detection device comprises a Raman probe, the Raman probe is provided with a probe head, and the probe head is inserted into the biological detection cabin; the biological detection cabin comprises a shell and a biological reaction substrate arranged in the shell, and drain holes are formed in the surface of the shell. After the in-situ biological detection system is assembled, the in-situ biological detection system dives to a required position, and the metabolic interaction process of organisms and the in-situ environment in the biological detection cabin is continuously monitored in real time according to the Raman detection device. The plug-in Raman probe in-situ biological detection system adopts a Raman in-situ detection method, is simpler in structure and stable in deep sea in-situ detection performance, and can meet the application requirements of sensing systems for in-situ detection of various organisms due to the in-situ environment detection of the system, so that the plug-in Raman probe in-situ biological detection system has more sensitive selectivity.

Description

Technical Field

[0001] The present invention relates to the technical field of deep-sea detection equipment, and more particularly to an in-situ biological detection system and detection method based on an inserted Raman probe. Background Art

[0002] In recent years, with the gradual development of deep-sea exploration with various detection technologies, Raman detection technology has been favored due to its advantages such as non-invasiveness, high sensitivity, high selectivity, and rapid analysis. Deep-sea in situ Raman detection technology is a technology that uses lasers to illuminate samples and measure changes in the intensity of scattered light, which is used to analyze the chemical composition and structure in deep-sea samples. The application range of deep-sea in situ Raman detection technology is very wide, including deep-sea geological research, deep-sea resource exploration, deep-sea environmental monitoring and other fields. However, there are still certain limitations in the study of deep-sea microorganisms. Due to the extremely harsh deep-sea environment and the wide variety of microorganisms, it is difficult to effectively analyze and process the weak in-situ microbial spectral signals. If the deep-sea microbial life metabolic process can be effectively detected in real time in situ, it will have more scientific reference significance for multiple disciplines. In view of this, deep-sea in situ microbial Raman detection technology is an urgent problem to be solved in this field. Summary of the invention

[0003] In view of this, the present invention provides an in-situ biological detection system and detection method based on an inserted Raman probe, which has more sensitive selectivity.

[0004] To achieve the above object, the present invention adopts the following technical solution:

[0005] Firstly, the present invention provides an in-situ biological detection system based on an insertable Raman probe, comprising: a Raman detection device and a biological detection chamber;

[0006] The Raman detection device comprises a Raman probe, wherein the Raman probe has a probe head, and the probe head is inserted into the biological detection chamber;

[0007] The biological detection cabin comprises an outer shell and a biological reaction base arranged in the outer shell, and a drainage hole is arranged on the surface of the outer shell.

[0008] Preferably, the Raman detection device is further provided with a light source coupling port and a collimated light path, and the detection light window of the Raman probe is a high-transmittance window.

[0009] Preferably, a fastening bayonet is provided on the inner wall at the top of the shell, and a protrusion is provided on the outer wall of the probe, and the protrusion matches the fastening bayonet.

[0010] Preferably, a fixed net is provided on the inner wall of the shell.

[0011] Preferably, the bioreactor substrate is wrapped with a biosemipermeable membrane.

[0012] Furthermore, the semipermeable membrane is one or more of an animal membrane, a cellophane, a cellulose derivative, and a synthetic polymer membrane.

[0013] Furthermore, the synthetic polymer membrane is one or more of polysulfone, polyethersulfone, polyamide, and polyvinylidene fluoride.

[0014] The detection method using the in-situ biological detection system described in the above technical solution includes the following steps:

[0015] After assembling the in-situ biological detection system, it dives to the required location, and uses the Raman detection device to continuously monitor the metabolic interaction process between the organisms in the biological detection cabin and the in-situ environment in real time.

[0016] It can be seen from the above technical solutions that, compared with the prior art, the present invention discloses an in-situ biological detection system and detection method based on an insertion Raman probe, which has the following beneficial effects:

[0017] The inserted Raman probe in-situ biological detection system adopts the Raman in-situ detection method, and its own structure is simpler and its deep-sea in-situ detection performance is stable. Since the system itself is an in-situ environmental detection, it can meet the application needs of the sensor system for in-situ detection of various types of organisms, making it more sensitive and selective. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0019] Figure 1 This is the overall structure diagram of the Raman detection device;

[0020] Figure 2 This is the structural diagram of the biological detection cabin;

[0021] Figure 3 This is a cross-sectional view of the biological detection cabin;

[0022] Figure 4 is a schematic diagram of an optical signal passing through a Raman detection device;

[0023] Figure 5 Schematic diagram of the structural matching between the probe and the housing.

[0024] In the figure, 111-Raman probe, 112-biological detection cabin, 113-protrusion; 211-housing, 212-fastening bayonet, 213-drainage hole, 311-fixing net, 312-biological reaction substrate, 411-glass chamber, 511-optical window. DETAILED DESCRIPTION

[0025] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] As attached Figure 1-3 As shown, the in-situ biological detection system based on the insertion type Raman probe comprises: a Raman detection device ( Figure 1 ) and biological detection cabin 112( Figure 1 and 2 -3);

[0027] The Raman detection device includes a Raman probe 111, wherein the Raman probe 111 has a probe head, and the probe head is inserted into the biological detection chamber;

[0028] The biological detection chamber 112 includes an outer shell 211 and a biological reaction base 312 placed in the outer shell 211. The surface of the outer shell 211 is provided with a drainage hole 213. The setting of the drainage hole 213 can accelerate the flow and discharge of seawater when used in situ, and is used to ensure the pressure release of the water body in the biological detection chamber and the interaction between the organisms in the chamber and the in-situ environment.

[0029] The Raman detection device is equipped with a light source coupling port, a collimated light path ( Figure 4 , wherein a glass device 411 and an optical window 511 are provided, the optical window 511 is a high-transmittance optical window, and the light signal passes through the optical coupling and collimation light path of the glass device 411 in the Raman probe in sequence, and then the excitation light is irradiated onto the sample from the high-transmittance optical window 511.

[0030] The top inner wall of the housing 211 is provided with a fastening bayonet 212 ( Figure 2 and 5 ), the outer wall of the probe is provided with a protrusion 113, which matches with the fastening bayonet 211 to prevent it from falling off and loosening.

[0031] A fixing net 311 is disposed on the inner wall of the housing 211 to protect and fix the bioreactor substrate 312 .

[0032] The bioreactor substrate 312 is an internal substance wrapped by a biological semipermeable membrane, and the substance to be tested is in the biological semipermeable membrane to ensure that bacteria and some metabolites will not be lost. The semipermeable membrane is one or more of an animal membrane, cellophane, a cellulose derivative, and a synthetic polymer membrane, and the synthetic polymer membrane is one or more of polysulfone, polyethersulfone, polyamide, and polyvinylidene fluoride.

[0033] When the Raman probe in-situ biological detection system is in use, the Raman detection device is connected to the biological detection cabin, which contains the observation samples required for deep-sea in-situ detection. Before the equipment dives, the biological reaction substrate, biological detection cabin, and Raman detection device are assembled in sequence. When the equipment dives to a certain environment and starts working, the Raman detection device can be used to continuously monitor the metabolic interaction process between the organisms in the biological reaction device and the in-situ environment in real time.

[0034] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0035] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An in-situ biological detection system based on an insertion Raman probe, characterized in that: include: Raman detection devices and biological detection chambers; The Raman detection device comprises a Raman probe, wherein the Raman probe has a probe head, and the probe head is inserted into the biological detection chamber; The biological detection cabin comprises an outer shell and a biological reaction base arranged in the outer shell, and a drainage hole is arranged on the surface of the outer shell.

2. The in-situ biological detection system based on an insertion Raman probe according to claim 1, characterized in that: The Raman detection device is also provided with a light source coupling port and a collimated light path, and the detection light window of the Raman probe is a high-transmittance window.

3. The in-situ biological detection system based on an insertion Raman probe according to claim 1, characterized in that: The top inner wall of the shell is provided with a fastening bayonet, and the outer wall of the probe is provided with a protrusion, and the protrusion matches the fastening bayonet.

4. The in-situ biological detection system based on an insertion Raman probe according to claim 1, characterized in that: The inner wall of the shell is provided with a fixed net.

5. The in-situ biological detection system based on an insertion Raman probe according to claim 1, characterized in that: The bioreaction substrate is wrapped by a biological semipermeable membrane.

6. The in-situ biological detection system based on an insertion Raman probe according to claim 5, characterized in that: The semipermeable membrane is one or more of an animal membrane, a cellophane, a cellulose derivative, and a synthetic polymer membrane.

7. The in-situ biological detection system based on an insertion Raman probe according to claim 6, characterized in that: The synthetic polymer membrane is one or more of polysulfone, polyethersulfone, polyamide and polyvinylidene fluoride.

8. A detection method using the in-situ biological detection system according to any one of claims 1 to 7, characterized in that: The following steps are involved: After assembling the in-situ biological detection system, it dives to the required location, and uses the Raman detection device to continuously monitor the metabolic interaction process between the organisms in the biological detection cabin and the in-situ environment in real time.

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