Agent for preventing and treating pine wood nematode disease through crown spraying absorption and preparation method thereof

Abstract

The invention provides a medicament for preventing and treating pine wood nematode disease through crown spraying absorption. The pesticide is characterized in that the pesticide is prepared from the following raw material components in parts by weight: 5 to 8 parts of 4 '-methylamino-4'-deoxyabamectin benzoate B145: 55), 8 to 15 parts of 1-(6-chloropyridine-3-pyridyl methyl)-N-nitroimidazolidine-2-ylamine, 10 parts of octylphenol polyoxyethylene ether and 75 parts of N, N-dimethylformamide. The invention also provides a preparation method of the medicament, which comprises the following steps: weighing N, N-dimethylformamide and putting into a stirring kettle; the preparation method comprises the following steps: weighing 4 '-methylamino-4'-deoxyabamectin benzoate B145: 55) and 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazolidine-2-ylamine, adding the materials into a stirring kettle, and stirring until the materials are completely dissolved; weighing octylphenol polyoxyethylene ether, adding the octylphenol polyoxyethylene ether into the stirring kettle, and uniformly stirring; and standing for 30 minutes. The pesticide is simple to construct, the efficiency is improved, the insecticidal effect is improved, the lasting time of the pesticide effect is prolonged, and the cost is greatly reduced.

Description

Technical Field

[0001] The invention relates to the technical field of pine wood insect prevention, in particular to an agent for preventing and treating pine wood nematode disease through crown spray absorption and a preparation method thereof. Background Art

[0002] Pine wilt disease is a very dangerous and destructive disease in forest ecosystems and a major plant epidemic. In order to control the spread of the disease, the country has invested a lot of financial resources. First, it kills longhorn beetles as a transmission medium by flying, second, it removes dead pine trees and strictly controls its application scope, and third, it carries out trunk injection prevention and control. However, the pine wood agent in the prior art has the problem of poor permeability. If it is only sprayed, it is difficult for the agent to penetrate into the leaves and trunks, the agent has a short residual time, and the prevention and control effect is very poor; in the prior art, in order to improve the effect of the agent, there is also a method of injecting the agent through the trunk, so that the agent enters the tree body and prolongs the effectiveness of the agent. However, the above method not only causes certain trauma to the tree body itself, but also has high labor cost, poor safety, and low efficiency in construction, and it cannot be implemented in some areas that are difficult for manpower to reach, thus causing a large number of dead pine trees in some forest areas. In order to remove these dead pine trees, manpower and material resources are further increased, and the cost of pine forest protection is increased. Summary of the invention

[0003] In response to the problems of the background technology, the present invention provides an agent for preventing and controlling pine wood nematode disease through crown spray absorption and a preparation method thereof, so as to solve the problems of poor permeability, short efficacy time, high treatment cost, poor safety and low efficiency of the prior art agents.

[0004] To achieve the purpose of the present invention, the present invention provides an agent for preventing and controlling pine wood nematode disease through crown spray absorption, and its innovation point is: the agent includes raw material components and weight fractions: 4'-methylamino-4'-deoxyavermectin benzoate B145:55) 5-8 parts, 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazole-2-ylamine 8-15 parts, octylphenol polyoxyethylene ether 10 parts, and N,N-dimethylformamide 75 parts.

[0005] The present invention also provides a preparation method for the above-mentioned medicament, the innovation of which is that the preparation method comprises:

[0006] S1. Weigh N,N-dimethylformamide and put it into a stirring kettle;

[0007] S2, weigh 4'-methylamino-4'-deoxyavermectin benzoate (B145:55) and 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazolidin-2-ylamine, add them into a stirred tank, and stir until they are completely dissolved;

[0008] S3, weigh octylphenol polyoxyethylene ether, add it into a stirring tank and stir evenly;

[0009] S4. Let stand for 30 minutes.

[0010] The principle of the present invention is as follows:

[0011] In the agent of the present invention, 4'-methylamino-4'-deoxyavermectin benzoate (B145:55) is a diatomite salt, which can kill the invading pine wood nematodes and inhibit the growth and reproduction of pine wood nematodes in pine trees; in order to improve the permeability of the agent, the present invention adds 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazole-2-ylamine, which is also an environmentally friendly agent component. It not only has an insecticidal effect on pine wood nematodes, but also has an insecticidal effect on pine wood nematodes. B145:55) has a strong killing effect on the pine beetle, the medium of pine nematodes, and while further improving the killing effect, it also has excellent drug permeability and up and down conductivity, thereby making the drug effect more lasting; further, the present invention also adds two auxiliary agents, octylphenol polyoxyethylene ether and N,N-dimethylformamide, among which the main function of N,N-dimethylformamide is to dissolve the above two insecticide components to form a flowing liquid, which is convenient for entering the tree body and the nutrients flowing in the pine tree The nutrient solution is mixed and then transported to the entire tree body; the role of octylphenol polyoxyethylene ether is mainly to improve the difference in interfacial tension between the leaf surface of the tree body and the agent described in the present application, so that the agent can quickly penetrate the surface of the needles into the tree body, mix with the tree body nutrient solution, and be transmitted to the entire tree body with the nutrient solution. It can be seen that the two can reduce the overall surface tension of the agent, enhance the pore and stomatal permeability of the agent, greatly improve the overall permeability of the agent, allow the agent to enter the trunk, thereby increasing the residual amount of the agent, and providing conditions for the agent to continue to exert its effectiveness, thereby greatly improving the effective time of the agent, greatly reducing the number of times the agent is applied (spraying once a year), and reducing the cost of treatment. At the same time, because the agent has strong penetration ability and long effective time, it is not necessary to perform trunk injection. It only needs to be sprayed with equipment such as drones to complete the application of the agent. The operation method is simple, the tree body itself can be completely non-traumatic, and the construction safety is good, the treatment efficiency is significantly improved, and the labor cost is greatly saved; on the other hand, it has been proved through experiments that the agent described in the present invention is environmentally friendly and safe to the pine tree itself, and there is no drug damage. Due to the above reasons, the pine tree disease and dieback rate in the pine forest has been greatly reduced, thereby reducing the cost of cleaning diseased trees and ecological restoration, and further reducing the protection cost of the pine forest.

[0012] It can be seen that the present invention has the following beneficial effects: the agent of the present invention has good permeability, increased efficacy time, simple treatment method, high efficiency, enhanced construction safety, environmental protection and safety. The use of the agent of the present invention for protecting pine forests not only greatly improves the insecticidal effect, the duration of the efficacy is significantly increased, and the cost is also greatly reduced. DETAILED DESCRIPTION

[0013] The present invention will be further described below in conjunction with the embodiments.

[0014] The pine wood nematode agent of the present invention comprises raw material components and weight fractions as follows: 4'-methylamino-4'-deoxyavermectin benzoate B145:55) 5-8 parts, 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazole-2-ylamine 8-15 parts, octylphenol polyoxyethylene ether 10 parts, and N,N-dimethylformamide 75 parts.

[0015] The preparation method of the present invention comprises:

[0016] S1. Weigh N,N-dimethylformamide and put it into a stirring kettle;

[0017] S2, weigh 4'-methylamino-4'-deoxyavermectin benzoate (B145:55) and 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazolidin-2-ylamine, add them into a stirred tank, and stir until they are completely dissolved;

[0018] S3, weigh octylphenol polyoxyethylene ether, add it into a stirring tank and stir evenly;

[0019] S4. Let stand for 30 minutes.

[0020] The following is an experiment on the therapeutic effect of the agent of the present invention, wherein INV-06 refers to the agent of the present invention; in the test data, emamectin benzoate refers to 4'-methylamino-4'-deoxyavermectin benzoate (B145:55), and imidacloprid refers to 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazole-2-ylamine.

[0021] 1. Determination of nematicidal activity:

[0022] The immersion method was used to dilute the test agent INV-06A formula (8% imidacloprid + 2% emamectin benzoate) and B formula (8% imidacloprid + 2% emamectin benzoate, octylphenol polyoxyethylene ether) into 6 concentration gradients (1mg / L, 0.5mg / L, 0.25mg / L, 0.125mg / L, 0.065mg / L, 0.03125mg / L) with distilled water for nematode activity determination. The results showed that different mass concentrations of INV-06A formula and B formula had a certain toxic effect on pine wood nematode (see Table 1). After 24h of treatment, the LC50 of A formula was 0.0916mg / L, and the LC50 of B formula was 0.1034mg / L (see Table 2).

[0023] Table 1 Corrected mortality of pine wood nematodes and mixed insects after 24 hours of INV-06 treatment

[0024]

[0025] Table 2 Virulence of pine wood nematode mixed insects after 24h treatment with INV-06

[0026]

[0027] 2. Systemic conduction effect:

[0028] The distribution and concentration changes of the active ingredients of INV-06 in plants after spraying the pesticide were detected by chemical analysis methods to verify its systemic conduction effect. The evaluation of the systemic conduction of the pesticide was carried out in Tianhai Management Area of ​​Huangshan Scenic Area, Anhui Province. The test results showed that after the application of different concentrations of INV-06, the content of emamectin benzoate and imidacloprid in the tree first increased and then decreased. The drug concentration was low at 15d (15 days), the drug content was the highest at 30d, and the drug content was lower at 90d than 30d (see Table 3). Although the content of emamectin benzoate in the tree could not reach its IC95 after 90d of application of the 60-fold diluted pesticide, the imidacloprid content was high, which could also achieve effective protection of pine trees. Therefore, different dilution concentrations of INV-06 had a good protective effect on Huangshan pine with a breast diameter of less than 80cm within 90d, but the 40-fold dilution concentration of the pesticide had the highest imidacloprid content at 90d for Huangshan pine with different breast diameters. Therefore, it is recommended to use a 40-fold dilution in subsequent experiments, which is also the most cost-effective.

[0029] Table 3 Contents of emamectin benzoate and imidacloprid in Pinus taiwanensis after treatment with different concentrations of INV-06

[0030]

[0031] Note: * indicates that the drug content in Pinus taiwanensis does not reach IC95.

[0032] 3. Residue detection:

[0033] The residual amounts of emamectin benzoate and imidacloprid in pine trees were detected 15 days, 1 month, 6 months, and 12 months after spraying, and the test results were statistically analyzed. As shown in Table 4, imidacloprid and emamectin benzoate can be detected in pine trees after spraying, and the systemic effect of imidacloprid on pine trees is better than that of emamectin benzoate. The higher the content of the agent, the better the systemic effect. Different dilution concentrations of INV-06 have a good protective effect on pine trees with a breast diameter of less than 80 cm within 90 days.

[0034] According to literature records, the LC95 value of the active ingredient of emamectin benzoate against pine wood nematodes is 0.031 mg / kg. In this experiment, one year after the foliar spraying, the residual amount of emamectin benzoate in the body was still greater than 0.031 mg / kg, that is, INV-06 can be absorbed by the needles, thus achieving the effect of completely non-invasive prevention and control of pine wood nematode disease in Masson pine.

[0035] Table 4 Residues of emamectin benzoate and imidacloprid in Pinus taiwanensis after treatment with different concentrations of INV-06

[0036]

[0037] 4. Dead tree survey:

[0038] Before spraying (end of October 2023), use drones to take orthophotos, count the number of diseased and dead trees per unit area, and conduct field inspections to check the condition of dead trees. At the end of October 2024, check the number of diseased and dead trees, and calculate the reduction rate of diseased and dead pine trees and the control effect. The calculation formula for the reduction rate of diseased and dead trees and the control effect is as follows:

[0039] The reduction rate of diseased and dead trees (%) = (the number of diseased and dead trees per unit area before prevention and control - the number of diseased and dead trees per unit area after prevention and control) / the number of diseased and dead trees per unit area before prevention and control = * 100%.

[0040] Control effect (%) = (reduction rate of diseased and dead trees in the control area - reduction rate of diseased and dead trees in the control area) / (100 - reduction rate of diseased and dead trees in the control area)*100.

[0041] (1) Muping Experimental Area

[0042] The survey results of the Mouping test area (see Table 5 and Table 6) show that INV-06 has a good control effect on pine wood nematode disease. After spraying with two different agents, the number of diseased and dead pine trees per hectare in the sample plot was significantly less than that in the clear water control treatment. The variance analysis showed that there were significant differences among the different treatments. In terms of control effect, treatment A (INV-06 agent 9 liters / hm 2 ) had the best treatment effect, with only 3.66 dead pine trees per unit area. 2 , compared with 21.15 strains / hm in the control area 2 Low 17.84 plants / hm2 Compared with 2023, the number of diseased and dead plants decreased by 82.70%, and the control effect was 81.28%; Treatment B (INV-06 agent was reduced by half (4.5 L / hm 2 ) and mixed with 8% cypermethrin microcapsule suspension 1.5 liters / hectare) was second in efficacy, with 12.17 dead pine trees per unit area / hm2 2 , compared with 21.15 strains / hm in the control area 2 Low 8.98 plants / hm 2 , the prevention and control effect is 51.98%.

[0043] Table 5 Number of dead pine trees in the Muping test area

[0044]

[0045] Note: Different lowercase letters in the same column indicate significant differences among different treatments (P<0.05)

[0046] Table 6 Control effect in the experimental area of ​​Muping

[0047]

[0048] (2) Guangchang Experimental Area

[0049] In 2024, the temperature in the Guangchang test area was high, the rainfall was small, and the drought period was long, which had a great impact on the growth and development of pine trees. In 2024, the number of diseased and dead plants per unit area in the control area was as high as 17.88 plants / hm2. 2 , compared with 7.59 strains / hm2 in 2023 2 After INV-06 was sprayed by aircraft, the number of diseased and dead trees per unit area in the sprayed area (treatment A) in 2024 was 8.61 trees / hm2 2 , compared with 7.32 strains / hm2 in 2023 2 The decrease was 17.62%, compared with 17.88 plants / hm2 in the control area. 2 It was reduced by 51.85% and the control effect was 61.24%, indicating that spraying INV-06 had a significant effect on the control of pine wood nematode disease (see Table 7).

[0050] Table 7 Number of diseased and dead plants in the experimental area of ​​Guangchang County

[0051]

[0052] 5. Safety evaluation

[0053] In 2023 and 2024, the safety evaluation of INV-06 was carried out in Huangshan Scenic Area and Guangchang County, Jiangxi Province, respectively. Within 7 days, 15 days, 1 month and 3 months after application, the pine needles of the treated Huangshan pine and Masson pine did not turn yellow or change color, and there was no significant difference between the branches and leaves and the clear water control. The agent is safe for Huangshan pine and Masson pine.

Claims

1. A drug for preventing and controlling pine wood nematode disease by spraying and absorbing it through the crown of a tree, characterized in that: The raw material components and weight fractions of the agent are: 5-8 parts of 4'-methylamino-4'-deoxyavermectin benzoate B145:55), 8-15 parts of 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazole-2-ylamine, 10 parts of octylphenol polyoxyethylene ether, and 75 parts of N,N-dimethylformamide.

2. A method for preparing a drug for preventing and treating pine wood nematode disease by spraying and absorbing the drug through the crown of a tree, characterized in that: The raw material components and weight fractions of the medicament are: 4'-methylamino-4'-deoxyavermectin benzoate B145:55) 5-8 parts, 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazole-2-ylamine 8-15 parts, octylphenol polyoxyethylene ether 10 parts, N,N-dimethylformamide 75 parts; the preparation method comprises: S1. Weigh N,N-dimethylformamide and put it into a stirring kettle; S2, weigh 4'-methylamino-4'-deoxyavermectin benzoate (B145:55) and 1-(6-chloropyridine-3-pyridylmethyl)-N-nitroimidazole-2-ylamine, add them into a stirring kettle, and stir until they are completely dissolved; S3, weigh octylphenol polyoxyethylene ether, add it into a stirring tank and stir evenly; S4. Let stand for 30 minutes.

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