Beeswax capable of dispelling cold, clearing damp and enhancing immunity as well as preparation method and application of beeswax

Abstract

The invention provides beeswax capable of fending off cold, clearing damp and enhancing immunity as well as a preparation method and application of the beeswax. The beeswax for dispelling cold, clearing damp and enhancing immunity is prepared from the following raw materials: beeswax, an absorption promoting agent, collagen peptide, a traditional Chinese medicine extracting solution and a solvent. The bee beewax capable of dispelling cold, clearing damp and enhancing immunity can improve the drug absorption capacity of a human body in a beewax therapy, so that the drug effect is enhanced, blood circulation is promoted, cold is dispelled, damp is dispelled, and the immunity is enhanced.

Description

Technical Field

[0001] The invention relates to the field of beeswax products, and in particular to beeswax capable of dispelling cold, removing dampness and enhancing immunity, and a preparation method and application thereof. Background Art

[0002] Beeswax is widely used in traditional medicine. In Shennong's Herbal Classic, beeswax is listed as a top-grade product with the effects of "curing diarrhea, pus and blood, replenishing the middle, healing broken wounds, wounds, and replenishing qi". In Compendium of Materia Medica, beeswax is recorded as "honey has a thick smell, so it nourishes the spleen. Wax has a thin smell, so it nourishes the stomach. Thick ones are sweet, and have a mild and soft texture, so they moisten the internal organs. Thin ones have a light taste, and have a hard and hard texture, so they stop diarrhea".

[0003] Beeswax is a fatty substance secreted by the wax glands in the abdomen of bees. It has warming, plasticity, moisturizing and antibacterial properties. In ancient times, people found that beeswax has warming, moisturizing and antibacterial effects, so they began to use beeswax to treat various diseases. Over time, beeswax therapy has continued to develop and improve. In the Middle Ages, beeswax therapy was widely used and became an important treatment method. Beeswax therapy is a local physical therapy that uses beeswax. In modern times, beeswax therapy has been further developed, and various different beeswax therapies have emerged, such as beeswax hot compress, beeswax massage, beeswax bath, etc. In modern times, as people pay more and more attention to health, traditional beeswax therapy has regained attention. More and more people are trying this natural therapy to relieve the various stresses and diseases brought about by modern life. Beeswax hot compress can relieve pain, muscle tension, arthritis and other diseases, and has a certain analgesic effect. After heating the beeswax and attaching it to the human skin, the beeswax releases heat, which increases the temperature of the local tissue, giving people a warm and comfortable feeling. However, the temperature of beeswax is high after heating, and it may cause burns if it is not handled properly. Beeswax uses the thermal effect to perform physical therapy on the skin surface. The therapeutic effect needs to be improved. When used in conjunction with traditional Chinese medicine, it enhances the skin's absorption of traditional Chinese medicine. However, the quality of beeswax is unstable, which results in general thermal insulation effect of beeswax, and unstable effects on promoting blood circulation, dispelling cold and dampness, and enhancing immunity. Summary of the invention

[0004] Purpose of the invention:

[0005] The purpose of the present invention is to provide a beeswax for dispelling cold, removing dampness and enhancing immunity, so as to solve the problem that the heat preservation effect of beeswax is general, the absorption capacity of human body to medicine and the ability to enhance the efficacy of medicine are weak, resulting in unstable effects on promoting blood circulation, dispelling cold, removing dampness and enhancing immunity.

[0006] The technical solution of the present invention:

[0007] A beeswax for dispelling cold, removing dampness and enhancing immunity, characterized in that the raw materials for preparing the beeswax for dispelling cold, removing dampness and enhancing immunity include: beeswax, an absorption promoter, collagen peptide, a traditional Chinese medicine extract, and a solvent.

[0008] Furthermore, the raw materials for preparing the beeswax for dispelling cold, removing dampness and enhancing immunity include, by weight: 50-70 parts of beeswax, 2-5 parts of absorption promoter, 1.5-2 parts of collagen peptide, 10-20 parts of traditional Chinese medicine extract, and 30-50 parts of solvent.

[0009] Furthermore, the preparation method of the absorption promoter is: first weigh 1,2-propylene glycol monolaurate and put it into a glass container, then weigh gingerol and vanillyl butyl ether respectively, add them slowly in sequence, and mix them evenly with a stirring rod.

[0010] Furthermore, the mass ratio of the gingerol, vanillyl butyl ether and 1,2-propylene glycol monododecanoate is 1:1-1.5:1-2.

[0011] Furthermore, the raw materials for preparing the traditional Chinese medicine extract include: mugwort leaf, dried ginger, cinnamon bark, atractylodes, atractylodes, tuckahoe, notopterygium wilfordii, and coix seed.

[0012] Furthermore, the mass ratio of the wormwood leaf, dried ginger, cinnamon bark, atractylodes, atractylodes macrocephala, tuckahoe, notopterygium wilfordii, and coix seed is 4-6:4-6:3-5:6-10:6-10:8-12:6-10:12-20.

[0013] Furthermore, the method for preparing the Chinese medicine extract comprises the following steps:

[0014] S1: Wash and dry Artemisia argyi, dried ginger, cinnamon bark, atractylodes, white atractylodes, Poria, Notopterygium wilfordii, and coix seed at low temperature, and then grind them respectively using a grinder, and then weigh and mix them according to a proportion to prepare the standby medicinal materials;

[0015] S2: Put the medicinal materials prepared in step S1 into a container, add an appropriate amount of water, heat and boil, first boil over high heat and then turn to low heat to keep it in a slightly boiling state, and boil for 1-2 hours;

[0016] S3: After the decoction in step S2 is completed, the filtrate is collected by filtering through gauze, and the filtrate is concentrated by a reduced pressure concentration method to obtain a Chinese medicine extract.

[0017] Furthermore, the solvent is one or a mixture of ethanol, tea oil, liquid paraffin and vaseline.

[0018] The present application also provides a method for preparing beeswax that can dispel cold, dampness and enhance immunity, which is characterized in that it includes the following steps: heating the beeswax to melt, then adding a solvent to dilute it, then adding a traditional Chinese medicine extract, stirring and mixing it evenly, then adding an absorbent promoter and collagen peptide, continuing to stir and mix it evenly, cooling it, and obtaining the beeswax that can dispel cold, dampness and enhance immunity.

[0019] The present application also provides an application of beeswax for dispelling cold, removing dampness and enhancing immunity, characterized in that the beeswax for dispelling cold, removing dampness and enhancing immunity is used for external application on the skin.

[0020] Furthermore, the application of beeswax for dispelling cold and dampness and enhancing immunity is characterized by adopting the following method:

[0021] Step 1: Put the beeswax into a heating device and heat it to 50-60℃ to make it liquid;

[0022] Step 2: Use a brush to evenly apply liquid beeswax on the patient's treatment area with a thickness of 1-2 cm. Cover with a layer of gauze after application and treat for 20-30 minutes. Alternatively, use a brush to apply beeswax that can dispel cold and dampness and enhance immunity on the treatment area of ​​the body, and then massage for 10-20 minutes.

[0023] The beeswax for dispelling cold and dampness and enhancing immunity of the present invention is added with an absorbent and a collagen peptide, which work together to enhance the absorption of drugs by the human body and enhance the efficacy. The mechanism of the absorbent for enhancing the absorption of drugs by the human body and enhancing the efficacy is: improving the solubility of the drug, the amphiphilicity of 1,2-propylene glycol monolaurate can help dissolve drug components with different lipophilicity and hydrophilicity. Its ester group can interact with lipophilic drugs, and the hydroxyl group can interact with hydrophilic drugs, so that the drugs are better dispersed in the system. The hydroxyl group of gingerol and the ether bond of vanillyl butyl ether also participate in this interaction, adjust the polarity of the system, improve the solubility of the drug, and facilitate the dissolution of the drug in the absorption site, thereby promoting absorption; adjust the permeability of the skin or mucous membrane, these components interact with the lipid layer of the skin or mucous membrane, and the ester group of 1,2-propylene glycol monolaurate can insert into the lipid bilayer of the skin or mucous membrane, change its fluidity and arrangement, and increase the channel for drug penetration. Gingerol and vanillyl butyl ether may also work synergistically by interacting with cell membrane components to temporarily increase the permeability of cell membranes, allowing drugs to enter cells or tissues more easily.

[0024] Promote drug diffusion: The mixture may reduce the surface tension of the drug at the absorption site. The interaction between 1,2-propylene glycol monolaurate, gingerol and vanillyl butyl ether can change the diffusion coefficient of the drug in the medium, making it easier for drug molecules to diffuse from high-concentration areas (such as the administration site) to low-concentration areas (such as blood circulation or target sites), accelerating the absorption rate of the drug and thus enhancing the efficacy; synergistic effect with the drug, these components interact physically and chemically with the drug molecules, changing the form or state of the drug, and making the drug more conducive to absorption and efficacy by forming complexes or changing the molecular conformation of the drug. They affect the metabolic process of the drug in the body, reduce the metabolic loss of the drug before entering the blood circulation, thereby increasing the bioavailability of the drug and ultimately enhancing the efficacy. At the same time, it also improves the heat preservation effect of beeswax, making the high temperature effective for a longer time, enhancing the penetration of active ingredients, improving the use effect of the product, enhancing the targeting of the drug, and improving the bioavailability of the drug. Collagen peptides are directly absorbed by skin cells, participate in the synthesis and repair process of collagen, provide nutrition to the skin, stimulate fibroblasts in the skin to secrete collagen and other extracellular matrix components, enhance the elasticity and toughness of the skin, and facilitate drug absorption.

[0025] Beneficial effects:

[0026] The beeswax for dispelling cold and dampness and enhancing immunity can improve the body's ability to absorb medicine in beeswax therapy, thereby enhancing the efficacy of the medicine, promoting blood circulation, dispelling cold and dampness, and enhancing immunity. DETAILED DESCRIPTION

[0027] The present invention will be described below in conjunction with specific embodiments. It should be noted that the following embodiments are examples of the present invention and are only used to illustrate the present invention, but not to limit the present invention. Other combinations and various modifications within the concept of the present invention may be performed without departing from the spirit or scope of the present invention.

[0028] Unless otherwise specified, the chemical reagents used in the present invention are all commercially available analytically pure.

[0029] Preparation method of absorbent:

[0030] First, weigh an appropriate amount of 1,2-propylene glycol monolaurate into a glass container, and then weigh gingerol and vanillyl butyl ether respectively according to the proportion, add them slowly in sequence, and stir with a stirring rod for 15 minutes to mix evenly.

[0031] Preparation of Chinese medicine extract A:

[0032] S1: Wash and dry wormwood, dried ginger, cinnamon, atractylodes, atractylodes, poria, angelica root and coix seed, and then grind them into powder using a grinder, weigh 40g of wormwood, 60g of dried ginger, 30g of cinnamon, 80g of atractylodes, 60g of atractylodes, 100g of poria, 60g of angelica root and 200g of coix seed, and mix them to prepare the standby medicinal materials;

[0033] S2: Put the medicinal materials prepared in step S1 into a container, add 1.5L of water, heat and boil, first boil over high heat and then turn to low heat to keep it in a slightly boiling state, and boil for 2 hours;

[0034] S3: After the decoction in step S2 is completed, the filtrate is collected by filtering through gauze, and the filtrate is concentrated by reduced pressure concentration at 0.08 MPa and 50°C to a liquid density of 1.5 g / mL to obtain a Chinese medicine extract A.

[0035] Preparation of Chinese medicine extract B:

[0036] The difference between the present preparation and the Chinese medicine extract A is that the 40g of mugwort, 60g of dried ginger, 30g of cinnamon, 80g of atractylodes, 60g of white atractylodes, 100g of Poria, 60g of Notopterygium, and 200g of coix seed weighed in step S1 are replaced with 60g of mugwort, 40g of dried ginger, 50g of cinnamon, 60g of atractylodes, 100g of white atractylodes, 80g of Poria, 100g of Notopterygium, and 120g of coix seed.

[0037] The preparation method of beeswax for dispelling cold and dampness and enhancing immunity comprises the following steps:

[0038] Heat the beeswax to 60°C to melt it, then add a solvent to dilute it, then add the Chinese medicine extract, stir and mix evenly, then add the absorption enhancer and collagen peptide, stir for 30 minutes, mix evenly, cool to room temperature, and you will get beeswax that can dispel cold and dampness and enhance immunity.

[0039] Examples 1 to 5

[0040] The preparation is carried out according to the above-mentioned preparation method of the absorbent and the beeswax for dispelling cold, dampness and enhancing immunity. Table 1 is the ingredient list of the beeswax for dispelling cold, dampness and enhancing immunity of Examples 1 to 5 in parts by weight.

[0041] Table 1 Ingredients of beeswax for dispelling cold and dampness and enhancing immunity of Examples 1 to 5

[0042]

[0043]

[0044] Comparative Example 1

[0045] Comparative Example 1 was prepared according to the above-mentioned method for preparing beeswax for dispelling cold, removing dampness and enhancing immunity. The difference between this preparation and Example 2 is that no absorption promoter is added.

[0046] Comparative Example 2

[0047] Comparative Example 2 was prepared according to the above-mentioned method for preparing beeswax for dispelling cold, removing dampness and enhancing immunity. The difference between this preparation and Example 2 is that the absorption promoter is replaced by hyaluronic acid.

[0048] Comparative Example 3

[0049] Comparative Example 3 was prepared according to the above-mentioned method for preparing beeswax for dispelling cold, removing dampness and enhancing immunity. The difference between this preparation and Example 2 is that no collagen peptide is added.

[0050] Performance test experiment:

[0051] 1. Heat preservation performance: The high temperature retention performance of beeswax was evaluated by using a temperature sensor and a timer to measure the duration for which the temperature of the products of Examples 1-5 and Comparative Examples 1-3 remained above 40°C after being heated to 55°C and melted.

[0052] 2. In vitro skin penetration experiment: Using a diffusion cell system, 2cm*5cm*10cm sized beeswax samples of Examples 1-5 and Comparative Examples 1-3 were heated to 55°C to melt and then placed in the donor chamber of the diffusion cell. The skin sample was placed in the middle of the diffusion cell. 10mL of phosphate buffer with a pH of 7.2 was added to the receptor chamber. After 30 minutes, samples were taken from the receptor chamber to analyze the content of traditional Chinese medicine ingredients in the phosphate buffer to evaluate the promoting effect of the cold-dispelling, dampness-removing and immunity-enhancing beeswax prepared in Examples 1-5 and Comparative Examples 1-3 on skin penetration.

[0053] 3. Determination of the activity of traditional Chinese medicine: respectively put the beeswax of embodiment 1-5 and comparative example 1-3 into a heating device, heat it to 55 ° C, melt it into liquid, and evenly apply the liquid beeswax to the treatment part of the patient with a brush, the thickness of which is 2 cm, cover it with a layer of gauze after application, treat for 30 minutes, and then test the IL-6 inflammatory factor level in the serum of the treatment part. The IL-6 inflammatory factor level in the blank group is 0.6 pg / mL, collect blood samples, collect the blood into a vacuum blood collection tube without anticoagulant, let the blood coagulate naturally, and then separate the serum by centrifugation, use the IL-6 ELISA detection kit, detect the absorbance according to the kit manual, draw a standard curve according to the absorbance value of the standard, calculate the concentration of IL-6 in the serum sample by the standard curve, and measure the IL-6 inflammatory factor level. Evaluate the improvement of the effect of beeswax on traditional Chinese medicine.

[0054] Performance test results Table 2:

[0055]

[0056] It can be seen from Table 2 that the beeswax for dispelling cold, removing dampness and enhancing immunity of the present invention has good heat preservation ability, can improve the body's absorption capacity of drugs and enhance drug efficacy. Specifically, it can be seen from the comparison between Example 4 and Example 2 that when preparing the absorption promoter, the unreasonable mass ratio of gingerol, vanillyl butyl ether and 1,2-propylene glycol monolaurate will lead to the weakening of the heat preservation ability of the beeswax for dispelling cold, removing dampness and enhancing immunity, the concentration of traditional Chinese medicine in the receiving solution will be lowered, and the improvement of the level of inflammatory factors will be weakened, and the expected effect will not be achieved; it can be seen from the comparison between Example 5 and Example 2 that when preparing the absorption promoter, if gingerol, vanillyl butyl ether and 1,2-propylene glycol monolaurate are not used at the same time, the heat preservation ability of the beeswax for dispelling cold, removing dampness and enhancing immunity will be weakened, and the concentration of traditional Chinese medicine in the receiving solution will be lowered. , the improvement of the level of inflammatory factors becomes weaker, and the expected effect cannot be achieved; it can be seen from Comparative Example 1 and Example 2 that when the absorption promoter is not added, the thermal insulation ability of beeswax for dispelling cold, dampness and immunity will deteriorate, the concentration of traditional Chinese medicine in the receiving liquid will be lower, the improvement of the level of inflammatory factors will deteriorate, and the expected effect cannot be achieved; Comparative Example 2 is compared with Example 2, and it can be seen that replacing the absorption promoter with commercially available hyaluronic acid will lead to the deterioration of the thermal insulation ability of beeswax for dispelling cold, dampness and immunity, the concentration of traditional Chinese medicine in the receiving liquid will be lower, the improvement of the level of inflammatory factors will be worse, and the expected effect cannot be achieved; Comparative Example 3 is compared with Example 2, and it can be seen that without adding collagen peptides, the thermal insulation ability of beeswax for dispelling cold, dampness and immunity will be deteriorated, the concentration of traditional Chinese medicine in the receiving liquid will be lower, the improvement of the level of inflammatory factors will be worse, and the expected effect cannot be achieved.

[0057] The present invention can also have many other embodiments. Without departing from the spirit and essence of the present invention, those skilled in the art can make various corresponding changes and modifications based on the present invention, but these corresponding changes and modifications should all fall within the scope of protection of the claims attached to the present invention.

Claims

1. A beeswax for dispelling cold and dampness and enhancing immunity, characterized in that: The raw materials for preparing the beeswax for dispelling cold, removing dampness and enhancing immunity include: beeswax, an absorption promoter, collagen peptide, a traditional Chinese medicine extract and a solvent.

2. The beeswax for dispelling cold and dampness and enhancing immunity according to claim 1, characterized in that: The raw materials for preparing the beeswax for dispelling cold, removing dampness and enhancing immunity include, by weight: 50-70 parts of beeswax, 2-5 parts of absorption promoter, 1.5-2 parts of collagen peptide, 10-20 parts of traditional Chinese medicine extract, and 30-50 parts of solvent.

3. The beeswax for dispelling cold and dampness and enhancing immunity according to claim 1, characterized in that: The preparation method of the absorption promoter is as follows: firstly weigh 1,2-propylene glycol monolaurate and put it into a glass container, then weigh gingerol and vanillyl butyl ether respectively, add them slowly in sequence, and mix them evenly with a stirring rod.

4. The beeswax for dispelling cold and dampness and enhancing immunity according to claim 3, characterized in that: The mass ratio of the gingerol, vanillyl butyl ether and 1,2-propylene glycol monolaurate is 1:1-1.5:1-2.

5. The beeswax for dispelling cold and dampness and enhancing immunity according to claim 1, characterized in that: The raw materials for preparing the traditional Chinese medicine extract include: wormwood leaf, dried ginger, cinnamon bark, atractylodes, atractylodes macrocephala, tuckahoe, notopterygium wilfordii and coix seed; the mass ratio of the wormwood leaf, dried ginger, cinnamon bark, atractylodes, atractylodes macrocephala, tuckahoe, notopterygium wilfordii and coix seed is 4-6:4-6:3-5:6-10:6-10:8-12:6-10:12-20.

6. The beeswax for dispelling cold and dampness and enhancing immunity according to claim 5, characterized in that: The method for preparing the Chinese medicine extract comprises the following steps: S1: Wash and dry Artemisia argyi, dried ginger, cinnamon bark, atractylodes, white atractylodes, Poria, Notopterygium wilfordii, and coix seed at low temperature, and then grind them respectively using a grinder, and then weigh and mix them according to a proportion to prepare the standby medicinal materials; S2: Put the medicinal materials prepared in step S1 into a container, add an appropriate amount of water, heat and boil, first boil over high heat and then turn to low heat to keep it in a slightly boiling state, and boil for 1-2 hours; S3: After the decoction in step S2 is completed, the filtrate is collected by filtering through gauze, and the filtrate is concentrated by a reduced pressure concentration method to obtain a Chinese medicine extract.

7. The beeswax for dispelling cold and dampness and enhancing immunity according to claim 1, characterized in that: The solvent is one or a mixture of ethanol, tea oil, liquid paraffin and vaseline.

8. The method for preparing the beeswax for dispelling cold, removing dampness and enhancing immunity according to any one of claims 1 to 7, characterized in that: The method comprises the following steps: heating and melting beeswax, adding a solvent to dilute it, adding a traditional Chinese medicine extract, stirring and mixing evenly, adding an absorbent and a collagen peptide, continuing to stir and mix evenly, cooling, and obtaining beeswax capable of dispelling cold, removing dampness and enhancing immunity.

9. The use of beeswax for dispelling cold, removing dampness and enhancing immunity according to any one of claims 1 to 7, characterized in that: Beeswax is used for external application on the skin to dispel cold and dampness and enhance immunity.

10. The use of beeswax for dispelling cold, removing dampness and enhancing immunity according to any one of claims 1 to 7, characterized in that: Use the following method: Step 1: Put the beeswax into a heating device and heat it to 50-60℃ to make it liquid; Step 2: Use a brush to evenly apply liquid beeswax on the patient's treatment area with a thickness of 1-2 cm. Cover with a layer of gauze after application and treat for 20-30 minutes. Alternatively, use a brush to apply beeswax that can dispel cold and dampness and enhance immunity on the treatment area of ​​the body, and then massage for 10-20 minutes.

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