Extraction and purification device for balsam pear ribosome inactivating protein and use method
By designing an extraction and purification device including a tank, a centrifuge, agitated crushing rod and an electric telescopic rod, the problem of difficult separation of bitter melon and extract in bitter melon ribosome in the extraction of bitter melon ribosome inactivated protein is solved, and high-purity protein extraction is achieved.
Patent Information
- Application Number
- CN202510003573.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-02
- Publication Date
- 2025-04-11
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the prior art, when extracting bitter melon ribosome inactivated protein, it is difficult to effectively separate bitter melon from the extract, resulting in the residual bitter melon to reduce the purity.
An extraction and purification device including a tank body, a centrifuge, agitating crushing rod and an electric telescopic rod is designed. By agitating the rotation of the crushing rod and the control of the electric telescopic rod, the crushing of bitter melon blocks and the submersion of liquid are achieved to avoid bitter melon residues.
Effectively separate bitter melon from the extract, improving the purity of bitter melon ribosome inactivated protein, and avoiding the interference of bitter melon residues on the suction head during the extraction process.
Smart Images
Figure CN119792991A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of extraction of bitter melon ribosome inactivating protein, and in particular to an extraction and purification device for bitter melon ribosome inactivating protein and a use method thereof. Background Art
[0002] Bitter melon ribosome inactivating proteins are a class of proteins extracted from bitter melon. They have N-glycosidase activity and can specifically act on the 28SrRNA of eukaryotic cell ribosomes, hydrolyzing one of the highly conserved adenine residues, thereby inactivating the ribosome and inhibiting protein synthesis. From the perspective of chemical structure, bitter melon ribosome inactivating proteins are generally single-chain or double-chain polypeptides with a relative molecular mass of about 25-30kDa.
[0003] When extracting bitter melon ribosome inactivating protein, an extraction and purification device is required. When the extraction and purification device is used, bitter melon and the extracting solution cannot be effectively separated. When extracting the extracting solution, a large amount of bitter melon will also be extracted, which brings inconvenience to the extraction of the extracting solution and reduces the purity of bitter melon ribosome inactivating protein. Therefore, in order to solve such problems, we propose an extraction and purification device for bitter melon ribosome inactivating protein and a method for use. Summary of the invention
[0004] The purpose of the present invention is to solve the shortcomings of the prior art and to propose an extraction and purification device for bitter melon ribosome inactivation protein and a method for use.
[0005] In order to achieve the above object, the present invention adopts the following technical solutions:
[0006] A device for extracting and purifying bitter melon ribosome inactivation protein comprises a tank body and a centrifuge, wherein a support ring is connected to the top of the inner wall of the tank body, a cover body is placed on the top of the support ring, a feed port is opened on one side of the top of the cover body, a conveying inclined plate used in conjunction with the feed port is connected to one side of the bottom of the cover body, a variable frequency drive motor is connected to the center of the top of the cover body, an output end of the variable frequency drive motor is connected to a drive shaft, the bottom of the drive shaft passes through the cover body and extends to the inside of the tank body, a plurality of stirring and crushing rods are connected to the outside of the drive shaft, a plurality of feeding tanks are connected to the outside of the top of the cover body, a tempered glass liquid level column is inlaid at one end of the outer side of the tank body, a filling pipe and a cleaning pipe are connected to the two ends of the top of one side of the tank body, and a restraining component is installed on the top of the inner wall of the tank body, the restraining component comprises a plurality of electric telescopic rods, a lifting ring and a cleaning sleeve, and the electric telescopic rod is located on the outside of the top of the cover body.
[0007] Preferably, a plurality of supporting legs are connected to the outer side of the bottom of the tank body, an outer pipe is connected to the center of the bottom of the tank body, an electric valve 1 is installed on the outer pipe, and the top of the tank body and the top of the cover body are on the same plane.
[0008] Preferably, the output end of the electric telescopic rod passes through the cover body and is connected to a T-block, the lifting ring is located at the top of the inner wall of the tank body, and a T-ring groove for use with the T-block is provided on the top of the lifting ring.
[0009] Preferably, one side of the cleaning sleeve is connected to the inner wall of the tank body, the top of the other side of the tank body is connected to a placement plate, the top of the placement plate is connected to a refrigerator, a delivery pipe is provided on one side of the refrigerator, the side of the delivery pipe away from the refrigerator is connected to a liquid pump, the liquid outlet end of the liquid pump is connected to a centrifuge, the side of the delivery pipe away from the liquid pump passes through the refrigerator and extends to the interior of the tank body and is connected to a telescopic tube, and a connecting ring is provided on the bottom sleeve of the telescopic end of one of the electric telescopic rods.
[0010] Preferably, the bottom of the telescopic tube is connected to a liquid suction head, one side of the connecting ring is connected to the liquid suction head, and a lifting notch for use with the connecting ring is provided through the cleaning sleeve.
[0011] Preferably, the inner wall of the lifting ring is connected to a plurality of shielding frames, a gap for the stirring and breaking rod to pass through is left between two adjacent shielding frames, and the inner side of the shielding frame is connected to the driving shaft.
[0012] Preferably, one side of the bottom of one of the shielding frames is connected to a support shaft, a connecting plate is provided on the outside of the support shaft through a bearing sleeve, a linear motor is connected to the side of the connecting plate away from the support shaft, an arc track is provided through the linear motor, both ends of the arc track are connected to support blocks, and the top of the support block is connected to the lifting ring
[0013] Preferably, one side of the connecting plate is connected to a shielding net, the other side of the shielding net is connected to the inner wall of the shielding frame, and the inner wall of the other shielding frame is also connected to a shielding net.
[0014] Preferably, the bottom of the feeding tank is connected to a feeding pipe, the bottom of the feeding pipe is connected to the cover body, two electric valves are installed on the feeding pipe, the outside of the feeding tank is inlaid with two tempered glass liquid level columns, the outside of the bottom of the feeding tank is connected to a number of support rods, and the bottom of the support rods is connected to the top of the cover body.
[0015] A method for using a device for extracting and purifying ribosome-inactivating protein from bitter melon, the method comprising the device for extracting and purifying ribosome-inactivating protein from bitter melon, and the method further comprising the following steps:
[0016] Step 1: Connect the filling pipe to the external pipeline, and add the cut bitter melon pieces into the tank through the feed port. After the bitter melon pieces are added, the external buffer solution is injected into the tank through the filling pipe. When the liquid level reaches the set height through the tempered glass level column, stop injecting the external buffer solution;
[0017] Step 2: Use the peripheral control end of the extraction and purification device to control the normal operation of the variable frequency drive motor, drive the drive shaft and the stirring and breaking rod to rotate, and use the shielding frame to drive the lifting and lowering ring to rotate. During the rotation of the stirring and breaking rod, the bitter melon pieces and the buffer solution are mixed and stirred. During the mixing process, two of the electric valves 2 are opened, and PMSF and β-mercaptoethanol in two of the tanks are added to the mixture of the bitter melon pieces and the buffer solution. At the same time, the breaking rod is stirred to fully break the bitter melon tissue and release the protein in the cells;
[0018] Step 3: After the release of the protein in the cell is completed, control the variable frequency drive motor to run slowly until the gap corresponds to the stirring and breaking rod. Control the linear motor to move outside the arc track, driving the connecting plate to rotate with the support shaft as the center to unfold the internal shielding net. The telescopic end of the electric telescopic rod extends out to drive the lifting ring to move downward. At this time, the stirring and breaking rod will pass through the gap. At the same time, the descending shielding net will press down the bitter melon fragments, causing a large amount of liquid to submerge the shielding net, and finally the gap will be stuck outside one of the sets of stirring and breaking rods, and the stirring and breaking rods will be used to complete the shielding of the gap;
[0019] Step 4: Open one of the electric valves 2, add the ammonium sulfate in the corresponding feed tank into the liquid, and turn on the variable frequency drive motor again to drive the stirring and breaking rod and the lifting ring to rotate, so that the liquid is mixed with the solid ammonium sulfate and other proteins are precipitated. In the process of further extension of the electric telescopic rod, the liquid suction head moves down through the cleaning sleeve, driving the telescopic tube to be stretched, and the liquid suction head enters the liquid. Turn on the refrigerator, centrifuge and liquid pump, and pump the liquid into the centrifuge to obtain high-purity bitter melon ribosome inactivation protein.
[0020] Compared with the prior art, the present invention has the following beneficial effects:
[0021] The present invention uses a cover body, a variable frequency drive motor, a feeding tank, a restraining component, a placement plate, a conveying inclined plate, a support ring, a stirring and breaking rod and a driving shaft in coordination. The variable frequency drive motor runs slowly until the gap corresponds to the stirring and breaking rod, and controls the linear motor to move outside the arc track, driving the connecting plate to rotate with the support shaft as the center of the circle to unfold the internal shielding net. The telescopic end of the electric telescopic rod extends out to drive the lifting ring to move downward, and the stirring and breaking rod will pass through the gap. At the same time, the descending shielding net presses down the bitter melon fragments, so that a large amount of liquid submerges the shielding net, and finally the gap is stuck outside one group of stirring and breaking rods. The stirring and breaking rods are used to complete the shielding of the gap, so as to avoid excessive bitter melon fragments from interfering with the use of the liquid suction head. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 It is a schematic diagram of the structure of the present invention;
[0023] Figure 2 For the present invention Figure 1 A front view of
[0024] Figure 3 It is a schematic diagram of the structure of the restraining component of the present invention;
[0025] Figure 4 For the present invention Figure 3 Bottom view of
[0026] Figure 5 For the present invention Figure 3 A magnified view of middle;
[0027] Figure 6 For the present invention Figure 4 Enlarged view of middle B;
[0028] Figure 7 This is a schematic diagram of the cover structure of the present invention;
[0029] Figure 8 For the present invention Figure 7 Bottom view of
[0030] Fig. 9 This is a schematic diagram of the structure coordination of the variable frequency drive motor, the stirring and breaking rod and the drive shaft of the present invention;
[0031] Fig.10 It is a schematic diagram of the structure of the feeding tank of the present invention.
[0032] In the figure: 1. tempered glass liquid level column 1; 2. tank body; 3. filling pipe; 4. feed port; 5. cleaning pipe; 6. cover body; 7. variable frequency drive motor; 8. feeding tank; 9. refrigerator; 10. delivery pipe; 11. centrifuge; 12. external discharge pipe; 13. electric valve 1; 14. restraining component; 141. T-block; 142. shielding net; 143. lifting ring; 144. arc track; 145. electric telescopic rod; 146. shielding frame; 147. T-ring groove; 148. support block; 149. linear motor; 1410. connecting plate; 1411. support shaft; 1412. connecting ring; 1413. cleaning sleeve; 1415. liquid suction head; 1416. telescopic tube; 15. placement plate; 16. delivery inclined plate; 17. support ring; 18. stirring and breaking rod; 19. drive shaft. DETAILED DESCRIPTION
[0033] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.
[0034] See also Figure 1-Figure 10A device for extracting and purifying bitter melon ribosome inactivation protein, comprising a tank body 2 and a centrifuge 11, a support ring 17 is connected to the top of the inner wall of the tank body 2, a cover body 6 is placed on the top of the support ring 17, a feed port 4 is opened on one side of the top of the cover body 6, a conveying inclined plate 16 used in conjunction with the feed port 4 is connected to one side of the bottom of the cover body 6, a variable frequency drive motor 7 is connected to the center of the top of the cover body 6, a drive shaft 19 is connected to the output end of the variable frequency drive motor 7, the bottom of the drive shaft 19 passes through the cover body 6 and extends To the interior of the tank body 2, the outside of the drive shaft 19 is connected to a plurality of stirring and breaking rods 18, the outside of the top of the cover body 6 is connected to a plurality of feeding tanks 8, one end of the outside of the tank body 2 is inlaid with a tempered glass liquid level column 1, and the two ends of the top of one side of the tank body 2 are respectively connected to the filling pipe 3 and the cleaning pipe 5, and a restraining component 14 is installed on the top of the inner wall of the tank body 2. The restraining component 14 includes a plurality of electric telescopic rods 145, a lifting ring 143 and a cleaning sleeve 1413, and the electric telescopic rod 145 is located on the outside of the top of the cover body 6.
[0035] As a technical optimization scheme of the present invention, a plurality of supporting legs are connected to the outer side of the bottom of the tank body 2, an outer discharge pipe 12 is connected to the center of the bottom of the tank body 2, an electric valve 13 is installed on the outer discharge pipe 12, and the top of the tank body 2 and the top of the cover body 6 are on the same plane; through the cooperation of the outer discharge pipe 12 and the electric valve 13, the discarded bitter gourd inside the tank body 2 can be conveniently discharged.
[0036] As a technical optimization solution of the present invention, the output end of the electric telescopic rod 145 passes through the cover body 6 and is connected to the T-block 141. The lifting ring 143 is located at the top of the inner wall of the tank body 2. The top of the lifting ring 143 is provided with a T-ring groove 147 for use with the T-block 141; the outside of the T-block 141 is in sliding contact with the inner wall of the T-ring groove 147.
[0037] As a technical optimization scheme of the present invention, one side of the cleaning sleeve 1413 is connected to the inner wall of the tank body 2, and the top of the other side of the tank body 2 is connected to a placement plate 15, and the top of the placement plate 15 is connected to a refrigerator 9. A delivery pipe 10 is provided on one side of the refrigerator 9, and the side of the delivery pipe 10 away from the refrigerator 9 is connected to a liquid pump, and the liquid outlet end of the liquid pump is connected to a centrifuge 11. The side of the delivery pipe 10 away from the liquid pump passes through the refrigerator 9 and extends to the interior of the tank body 2 and is connected to a telescopic tube 1416, and the bottom sleeve of the telescopic end of one of the electric telescopic rods 145 is provided with a connecting ring 1412; the refrigerator 9 can be supported by the placement plate 15, which is convenient for the installation of the refrigerator 9; the delivery pipe 10 is in a curved snake shape inside the refrigerator 9.
[0038] As a technical optimization solution of the present invention, the bottom of the telescopic tube 1416 is connected to the liquid suction head 1415, one side of the connecting ring 1412 is connected to the liquid suction head 1415, and a lifting notch is opened through the cleaning sleeve 1413 for use with the connecting ring 1412; through the lifting notch, the liquid suction head 1415 can be raised and lowered when the electric telescopic rod 145 is extended.
[0039] As a technical optimization solution of the present invention, the inner wall of the lifting ring 143 is connected to a plurality of shielding frames 146, and a gap is left between two adjacent shielding frames 146 for the stirring and breaking rod 18 to pass through, and the inner side of the shielding frame 146 is connected to the driving shaft 19; the gap facilitates the stirring and breaking rod 18 to pass through the shielding frame 146.
[0040] As a technical optimization solution of the present invention, one side of the bottom of a shielding frame 146 is connected to a support shaft 1411, and a connecting plate 1410 is provided on the outside of the support shaft 1411 through a bearing movable sleeve, and a linear motor 149 is connected to the side of the connecting plate 1410 away from the support shaft 1411, and an arc track 144 is penetrated by the linear motor 149, and both ends of the arc track 144 are connected to support blocks 148, and the top of the support block 148 is connected to the lifting ring 143; the arc track 144 can be positioned and supported by the support block 148.
[0041] As a technical optimization solution of the present invention, one side of the connecting plate 1410 is connected to a shielding net 142 , the other side of the shielding net 142 is connected to the inner wall of the shielding frame 146 , and the inner walls of other shielding frames 146 are also connected to shielding nets 142 .
[0042] As a technical optimization solution of the present invention, the bottom of the feeding tank 8 is connected to a feeding pipe, the bottom of the feeding pipe is connected to the cover body 6, and two electric valves are installed on the feeding pipe. The outside of the feeding tank 8 is inlaid with two tempered glass liquid level columns, and the outside of the bottom of the feeding tank 8 is connected to a number of support rods, and the bottom of the support rods is connected to the top of the cover body 6; through the two tempered glass liquid level columns, the liquid level inside the feeding tank 8 is conveniently penetrated.
[0043] A method for using a device for extracting and purifying ribosome-inactivating protein from bitter melon, the method comprising the device for extracting and purifying ribosome-inactivating protein from bitter melon, and the method further comprising the following steps:
[0044] Step 1: The filling pipe 3 is connected to the external pipeline, and the cut bitter melon pieces are added into the tank body 2 through the feed port 4. During the adding process, due to the presence of the conveying inclined plate 16, the bitter melon pieces will fall to the middle of the tank body 2 to prevent the bitter melon pieces from accumulating at the edge of the tank body 2. In the subsequent mixing process of the bitter melon pieces with the buffer solution, due to the shielding of the conveying inclined plate 16, the splashed liquid will not splash from the feed port 4 to the external environment. After the bitter melon pieces are added, the external buffer solution is injected into the tank body 2 through the filling pipe 3. After the liquid level reaches the set height through the tempered glass liquid level column 1, the injection of the external buffer solution is stopped. The buffer solution includes Tris-HCl, NaCl and EDTA.
[0045] Step 2: Use the external control end of the extraction and purification device to control the variable frequency drive motor 7 to operate normally, drive the drive shaft 19 and the stirring and breaking rod 18 to rotate, and use the shielding frame 146 to drive the lifting and lowering ring 143 to rotate. During the rotation of the stirring and breaking rod 18, the bitter melon pieces and the buffer solution are mixed and stirred. During the mixing process, two of the electric valves 2 are opened, and PMSF and β-mercaptoethanol in two of the tanks 2 are added to the mixture of the bitter melon pieces and the buffer solution. At the same time, the stirring and breaking rod 18 is used to fully break the bitter melon tissue and release the protein in the cells.
[0046] Step 3: After the release of the protein in the cell is completed, the variable frequency drive motor 7 is controlled to run slowly until the gap corresponds to the stirring and breaking rod 18. The linear motor 149 is controlled to move outside the arc track 144, driving the connecting plate 1410 to rotate around the support shaft 1411 as the center of the circle to unfold the internal shielding net 142. The telescopic end of the electric telescopic rod 145 extends to drive the lifting ring 143 to move downward. At this time, the stirring and breaking rod 18 will pass through the gap, and the descending shielding net 142 will press down the bitter melon pieces, so that a large amount of liquid will submerge the shielding net 142, and finally the gap will be stuck outside one of the groups of stirring and breaking rods 18, and the stirring and breaking rods 18 will be used to complete the shielding of the gap.
[0047] Step 4: Open one of the electric valves 2, add the ammonium sulfate in the corresponding feed tank 8 into the liquid, and turn on the variable frequency drive motor 7 again to drive the stirring and breaking rod 18 and the lifting ring 143 to rotate, so that the liquid is mixed with the solid ammonium sulfate and other proteins are precipitated. In the process of further extending the electric telescopic rod 145, the liquid suction head 1415 moves down and passes through the cleaning sleeve 1413, driving the telescopic tube 1416 to be stretched, and the liquid suction head 1415 enters the liquid, and the refrigerator 9, the centrifuge 11 and the liquid pump are turned on to pump the liquid into the centrifuge 11 for centrifugation to obtain high-purity bitter melon ribosome inactivation protein.
[0048] Step 5: If the liquid suction head 1415 is blocked during the liquid suction process, the electric telescopic rod 145 is controlled to continuously retract and extend, and the cleaning sleeve 1413 is used to clean the outside of the liquid suction head 1415 so that it can be used for a long time. After the liquid suction is completed, the electric telescopic rod 145 is retracted, driving the corresponding components to reset, and then the variable frequency drive motor 7 runs at high speed, and the bitter melon fragments remaining on the top of the shielding net 142 are thrown out and dropped to the bottom of the inner wall of the tank body 2. At the same time, clean water is injected into the tank body 2 through the filling pipe 3 to clean the inside of the tank body 2, and the electric valve 13 is opened to discharge the bitter melon fragments and the cleaned water.
[0049] The above are only preferred specific implementation modes of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.
Claims
1. A device for extracting and purifying bitter melon ribosome inactivating protein, comprising a tank (2) and a centrifuge (11), characterized in that: The top of the inner wall of the tank body (2) is connected to a support ring (17), a cover body (6) is placed on the top of the support ring (17), a feed port (4) is opened on one side of the top of the cover body (6), a conveying inclined plate (16) used in conjunction with the feed port (4) is connected to one side of the bottom of the cover body (6), a variable frequency drive motor (7) is connected to the center of the top of the cover body (6), an output end of the variable frequency drive motor (7) is connected to a drive shaft (19), the bottom of the drive shaft (19) passes through the cover body (6) and extends to the inside of the tank body (2), and the outer side of the drive shaft (19) is connected to the inner side of the cover body (6). The top of the tank body (2) is connected to a plurality of stirring and crushing rods (18), the outer side of the top of the cover body (6) is connected to a plurality of feeding tanks (8), one end of the outer side of the tank body (2) is inlaid with a tempered glass liquid level column (1), the two ends of the top of one side of the tank body (2) are respectively connected to a filling pipe (3) and a cleaning pipe (5), and a restraining component (14) is installed on the top of the inner wall of the tank body (2), and the restraining component (14) includes a plurality of electric telescopic rods (145), a lifting ring (143) and a cleaning sleeve (1413), and the electric telescopic rod (145) is located on the outer side of the top of the cover body (6).
2. The extraction and purification device for bitter melon ribosome inactivating protein according to claim 1, characterized in that: The outer side of the bottom of the tank body (2) is connected to a plurality of supporting legs, the center of the bottom of the tank body (2) is connected to an outer pipe (12), an electric valve (13) is installed on the outer pipe (12), and the top of the tank body (2) and the top of the cover body (6) are on the same plane.
3. The device for extracting and purifying bitter melon ribosome inactivating protein according to claim 1, characterized in that: The output end of the electric telescopic rod (145) passes through the cover body (6) and is connected to a T-shaped block (141). The lifting ring (143) is located at the top of the inner wall of the tank body (2). The top of the lifting ring (143) is provided with a T-shaped ring groove (147) for use with the T-shaped block (141).
4. The device for extracting and purifying bitter melon ribosome inactivating protein according to claim 1, characterized in that: One side of the cleaning sleeve (1413) is connected to the inner wall of the tank body (2), and the top of the other side of the tank body (2) is connected to a placement plate (15), and the top of the placement plate (15) is connected to a refrigerator (9). A delivery pipe (10) is provided on one side of the refrigerator (9), and a liquid extraction pump is connected to the side of the delivery pipe (10) away from the refrigerator (9), and the liquid outlet end of the liquid extraction pump is connected to a centrifuge (11). The side of the delivery pipe (10) away from the liquid extraction pump passes through the refrigerator (9) and extends to the inside of the tank body (2) and is connected to a telescopic pipe (1416), and a connecting ring (1412) is provided at the bottom of the telescopic end of one of the electric telescopic rods (145).
5. The device for extracting and purifying bitter melon ribosome inactivating protein according to claim 4, characterized in that: The bottom of the telescopic tube (1416) is connected to a liquid suction head (1415), one side of the connecting ring (1412) is connected to the liquid suction head (1415), and a lifting notch for use with the connecting ring (1412) is provided through the cleaning sleeve (1413).
6. The device for extracting and purifying bitter melon ribosome inactivating protein according to claim 1, characterized in that: The inner wall of the lifting ring (143) is connected to a plurality of shielding frames (146), a gap for the stirring and breaking rod (18) to pass through is left between two adjacent shielding frames (146), and the inner side of the shielding frame (146) is connected to the driving shaft (19).
7. The device for extracting and purifying bitter melon ribosome inactivating protein according to claim 6, characterized in that: A support shaft (1411) is connected to one side of the bottom of one of the shielding frames (146); a connecting plate (1410) is provided on the outside of the support shaft (1411) via a bearing sleeve; a linear motor (149) is connected to the side of the connecting plate (1410) away from the support shaft (1411); an arc track (144) is provided through the linear motor (149); both ends of the arc track (144) are connected to support blocks (148); and the top of the support block (148) is connected to the lifting ring (143).
8. The device for extracting and purifying bitter melon ribosome inactivating protein according to claim 7, characterized in that: One side of the connecting plate (1410) is connected to a shielding net (142), the other side of the shielding net (142) is connected to the inner wall of the shielding frame (146), and the inner walls of other shielding frames (146) are also connected to shielding nets (142).
9. The device for extracting and purifying bitter melon ribosome inactivating protein according to claim 1, characterized in that: The bottom of the feeding tank (8) is connected to a feed pipe, the bottom of the feed pipe is connected to the cover body (6), two electric valves are installed on the feed pipe, the outside of the feeding tank (8) is inlaid with two tempered glass liquid level columns, the outside of the bottom of the feeding tank (8) is connected to a plurality of support rods, and the bottom of the support rods is connected to the top of the cover body (6).
10. A method for using a device for extracting and purifying bitter melon ribosome inactivating protein, characterized in that: The method of use comprises the extraction and purification device for bitter melon ribosome inactivating protein in claims 1 to 9, and the method of use further comprises the following steps: Step 1: The filling pipe (3) is connected to the external pipeline, and the cut bitter melon pieces are added into the tank body (2) through the feed port (4). After the bitter melon pieces are added, the external buffer is injected into the tank body (2) through the filling pipe (3). After the liquid level reaches the set height through the tempered glass liquid level column 1 (1), the injection of the external buffer is stopped; Step 2: Use the peripheral control end of the extraction and purification device to control the normal operation of the variable frequency drive motor (7), drive the drive shaft (19) and the stirring and breaking rod (18) to rotate, and use the shielding frame (146) to drive the lifting and lowering ring (143) to rotate. During the rotation of the stirring and breaking rod (18), the bitter melon pieces and the buffer solution are mixed and stirred. During the mixing process, two of the electric valves 2 are opened, and PMSF and β-mercaptoethanol in two of the tanks (2) are added to the mixture of the bitter melon pieces and the buffer solution. At the same time, the stirring and breaking rod (18) is used to fully break the bitter melon tissue and release the protein in the cells; Step three: After the release of the protein in the cell is completed, control the variable frequency drive motor (7) to run slowly until the gap corresponds to the stirring and crushing rod (18). Control the linear motor (149) to move outside the arc track (144), driving the connecting plate (1410) to rotate around the support shaft (1411) to unfold the internal shielding net (142). The telescopic end of the electric telescopic rod (145) extends to drive the lifting ring (143) to move downward. At this time, the stirring and crushing rod (18) will pass through the gap, and at the same time, the descending shielding net (142) will press down the bitter melon fragments, so that a large amount of liquid submerges the shielding net (142), and finally the gap is stuck outside one of the groups of stirring and crushing rods (18), and the stirring and crushing rods (18) are used to complete the shielding of the gap; Step 4: Open one of the electric valves 2, add the ammonium sulfate in the corresponding feed tank (8) into the liquid, and turn on the variable frequency drive motor (7) again to drive the stirring and breaking rod (18) and the lifting ring (143) to rotate, so that the liquid and the solid ammonium sulfate are mixed and other proteins are precipitated. Further, when the electric telescopic rod (145) is extended, the liquid suction head (1415) moves down and passes through the cleaning sleeve (1413), driving the telescopic tube (1416) to be stretched, and the liquid suction head (1415) enters the liquid, and the refrigerator (9), the centrifuge (11) and the liquid pump are turned on to pump the liquid into the centrifuge (11) for centrifugation to obtain high-purity bitter melon ribosome inactivation protein.