A split-type noise reduction and vibration damping optical platform for patch clamp experiments

Abstract

This invention discloses a split-type noise reduction and vibration damping optical platform for patch clamp experiments, comprising: a buoyancy tank, a base plate platform, and an operating platform; a movable base plate is provided at the inner bottom of the buoyancy tank, and the circumferential side of the movable base plate abuts against the inner wall of the buoyancy tank to form a seal; a damping spring is provided on the base plate platform, penetrating the bottom of the buoyancy tank; a bent rod is fixedly provided on the bottom side of the movable base plate, one end of the bent rod is hinged to a vertical rod, and one end of the vertical rod is hinged to a swing arm; the end of the swing arm is provided with an active tooth that rotates with it; a driven tooth is provided on a second support rod, and a linkage rod is provided on one side of the driven tooth; one end of the linkage rod is fixedly connected to the movable base plate, and the active tooth and rack respectively mesh with the driven tooth. This invention enables the movable base plate and the sealing plate to be in a linked state, allowing the fluid in the divided cavity to directly enter / exit the second hole, while the volume of fluid remaining in the first hole remains unaffected, ensuring the stability of the float position in the first hole and avoiding interference from signal noise during the experiment.

Description

Technical Field

[0001] This invention relates to the field of operating platform technology for patch clamp experiments, and specifically to a separate noise reduction and vibration damping optical platform for patch clamp experiments. Background Technology

[0002] Patch-clamp technique is an electrophysiological research tool that can reveal the electrophysiological characteristics of cells by recording and analyzing ion channels on the cell membrane, providing important support for research in multiple fields such as neuroscience, pharmacology, and cell biology.

[0003] The patch-clamp optical experimental platform is an experimental system combining patch-clamp technology and optical imaging technology, used to study the physiological functions of cells and ion channels. It can simultaneously measure the electrophysiological and optical properties of cell membranes. Through patch-clamp experiments, the current passing through ion channels or changes in cell membrane potential can be measured; optical imaging technology allows for the observation of cell structure and function. However, patch-clamp experimental platforms suffer from signal noise during use. Mechanical vibrations from inside and outside the laboratory, traffic vibrations, and building vibrations can all be transmitted to the patch-clamp experimental apparatus through the platform, causing signal noise. The mechanical noise of the equipment itself, such as the mechanical vibrations generated by microscopes, micromanipulators, and pumps during operation, can also become a source of signal noise.

[0004] Microcurrent measurements in patch-clamp experiments are typically very sensitive, thus requiring a reliable patch-clamp experimental platform to ensure the accuracy and reliability of the experiments. Summary of the Invention

[0005] The purpose of this invention is to provide a separate noise reduction and vibration damping optical platform for patch clamp experiments, so as to solve the technical problem that signal noise interference caused by the transmission of vibration from the environment to the experimental platform during the experiment can affect the accuracy and reliability of the experimental results.

[0006] To solve the above-mentioned technical problems, the present invention specifically provides the following technical solution:

[0007] This invention provides a split-type noise reduction and vibration damping optical platform for patch clamp experiments, comprising: a buoyancy tank, inside which a partition is fixedly installed, the partition being arranged along the cross-section of the buoyancy tank, dividing the inner cavity of the buoyancy tank into a sub-cavity located below the partition, the sub-cavity being used to fill fluid; a first hole and a second hole are provided through the partition, the second hole containing a movable sealing plate, the fluid in the sub-cavity being forced into the first hole and the second hole by external pressure; a movable base plate is provided at the inner bottom of the buoyancy tank, the circumferential side of the movable base plate forming a seal with the inner wall of the buoyancy tank; a base plate platform, the outer bottom of the buoyancy tank being supported and fixed to the base plate platform by a shock-absorbing column, the base plate platform having a damping spring penetrating through the bottom of the buoyancy tank, the damping spring supporting the movable base plate and the inner bottom of the buoyancy tank. The vibration experienced by the base plate platform is weakened by the damping column and then transmitted to the buoyancy tank, where it is absorbed by the fluid in the compartment. An operating platform is located above and separated from the buoyancy tank. A floating base column is fixedly connected to the bottom of the operating platform, and a float plate is fixedly connected below the floating base column. The float plate is movably positioned within the first closed hole. A bent rod is fixedly mounted on the bottom side of the movable base plate. A vertical rod is hinged to one end of the bent rod, and a swing arm is hinged to one end of the vertical rod. A support rod is mounted on the outer wall of the buoyancy tank. The support rod is axially connected to one end of the swing arm. The end of the swing arm has a driving tooth that rotates with it. A second support rod is fixedly mounted on the first support rod, and a driven tooth is mounted on the second support rod. A linkage rod is mounted on one side of the driven tooth. One end of the linkage rod is fixedly connected to the movable base plate, and the other end of the linkage rod has a rack. The driving tooth and the rack mesh with the driven tooth, respectively.

[0008] Furthermore, the first hole is located in the middle of the partition, and the second hole is located on the annular path around the first hole.

[0009] Furthermore, the area of ​​the movable base plate is S, the area of ​​the first hole is S1, the area of ​​the second hole is S2, and the transmission ratio of the driving tooth and the driven tooth is S2:S, so that the fluid volume change in the sub-cavity is consistent with the fluid volume change in the second hole.

[0010] Furthermore, the end of the swing arm is keyed to the drive gear.

[0011] Furthermore, a limiting block is provided on the inner wall of the second hole, and the limiting block is located near the lower end of the second hole; wherein, a limiting groove is provided on the sealing plate, the limiting groove is located on the lower surface of the sealing plate, and the limiting block is positioned corresponding to the limiting groove so as to be embedded therein; the limiting groove is circumferentially equidistantly provided at the edge of the sealing plate, and the number of limiting blocks and limiting grooves is at least 2, and the number of limiting grooves is not less than the number of limiting blocks.

[0012] Furthermore, the upper surface of the sealing plate is provided with a telescopic rod, which is located near the edge of the sealing plate. The telescopic rod is used to keep the sealing plate in a floating path in the vertical direction. One end of the telescopic rod is fixedly connected to the sealing plate, and a limiting ring is provided on the inner wall of the second hole. The other end of the telescopic rod is located on the limiting ring.

[0013] Furthermore, a connecting hole is provided on the limiting ring, and a sliding top block and a limiting body are provided in the connecting hole. The top block is used to connect the telescopic rod. As the telescopic rod is subjected to force, the top block gradually slides upward and stops against the limiting body, and the base of the telescopic rod retracts into the connecting hole.

[0014] Furthermore, the top surface of the floating base column is higher than the top edge of the buoyancy tank, so that the operating platform is separated from the buoyancy tank.

[0015] Furthermore, the baffle is located at the middle height of the inner cavity of the buoyancy tank.

[0016] Furthermore, a damping ring is fitted onto the damping column, with the damping ring positioned near the bottom of the damping column and in contact with the upper surface of the substrate platform to reduce the vibration transmitted from the substrate platform to the damping column.

[0017] Compared with the prior art, the present invention has the following advantages:

[0018] 1. This invention, through the setting of a buoyancy tank, allows the experimental operating platform to be separated from the base plate platform used to place the equipment. The buoyancy generated by the fluid substance filled in the buoyancy tank provides floating support for the operating platform, avoiding direct and indirect contact between the operating platform and the base plate platform. It also significantly reduces the energy transmitted from the base plate platform to the operating platform, and avoids signal noise caused during the experiment from interfering with the patch clamp experiment. Thus, it can maximize the smooth progress of the patch clamp experiment and solve the problem of signal noise affecting microcurrents during the use of the patch clamp experimental operating platform.

[0019] 2. This invention uses the meshing transmission between the active and driven teeth to keep the movable base plate and the sealing plate in a linked state. This allows the movable base plate to drive the sealing plate to move during vibration, allowing the fluid in the cavity to directly enter / exit the second hole, while the volume of fluid remaining in the first hole remains unaffected. This keeps the position of the float plate in the first hole highly stable and improves the stability of the experimental platform. Attached Figure Description

[0020] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0021] Figure 1 A three-dimensional structural diagram of a split-type noise reduction and vibration damping optical platform for patch clamp experiments provided by the present invention;

[0022] Figure 2 for Figure 1 A sectional view;

[0023] Figure 3 for Figure 2 Enlarged view of section I;

[0024] Figure 4 for Figure 2 Enlarged view of section II;

[0025] Figure 5 for Figure 2 Enlarged view of section III;

[0026] Figure 6 for Figure 5 A schematic diagram and a partial view of the structure in another embodiment of the present invention.

[0027] The labels in the diagram represent the following:

[0028] 1. Buoyancy tank body; 11. Baffle plate; 12. Divided cavity; 13. First hole; 14. Second hole; 141. Limiting block; 15. Sealing plate; 151. Limiting groove; 16. Movable base plate; 161. Bent rod; 162. Vertical rod; 163. Swing arm; 164. Support rod one; 165. Driving gear; 166. Support rod two; 167. Driven gear; 168. Linkage rod; 169. Rack; 17. Connecting hole; 171. Top block; 172. Limiting body; 18. Limiting ring; 2. Base plate platform; 21. Shock-absorbing column; 22. Damping spring component; 23. Shock-absorbing ring; 3. Operating platform; 31. Floating base column; 32. Floating plate; 33. Telescopic rod component. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] like Figure 1-3 As shown, the present invention provides a split-type noise reduction and vibration damping optical platform for patch clamp experiments, comprising: a buoyancy tank 1, wherein a partition 11 is fixedly provided inside the buoyancy tank 1, the partition 11 is arranged along the cross-section of the buoyancy tank 1, the partition 11 divides the inner cavity of the buoyancy tank 1 into a sub-cavity 12 located below the partition 11, the sub-cavity 12 is used to fill fluid; a first hole 13 and a second hole 14 are provided through the partition 11, a movable sealing plate 15 is provided in the second hole 14, and the fluid in the sub-cavity 12 can be forced into the first hole 13 and the second hole 14 by external pressure; a movable bottom plate 16 is provided at the inner bottom of the buoyancy tank 1, and a seal is formed between the circumferential side of the movable bottom plate 16 and the inner wall of the buoyancy tank 1;

[0031] The base plate platform 2 and the outer bottom of the buoyancy tank 1 are supported and fixed on the base plate platform 2 by the shock-absorbing column 21. The base plate platform 2 is provided with a damping spring 22 that penetrates the bottom of the buoyancy tank 1. The damping spring 22 supports the movable bottom plate 16 to be separated from the inner bottom of the buoyancy tank 1. The vibration of the base plate platform 2 is weakened by the shock-absorbing column 21 and then transmitted to the buoyancy tank 1 and absorbed by the fluid in the cavity 12.

[0032] Operating platform 3 is located above and separated from buoyancy tank 1. A floating base column 31 is fixedly connected to the lower part of the operating platform 3. A floating plate 32 is fixedly connected to the lower part of the floating base column 31. The floating plate 32 is movably located inside the first hole 13.

[0033] More specifically, a bent rod 161 is fixed to the bottom side of the movable base plate 16. One end of the bent rod 161 is hinged to a vertical rod 162, and the other end of the vertical rod 162 is hinged to a swing arm 163. A support rod 164 is provided on the outer wall of the buoyancy tank 1. The support rod 164 is axially connected to one end of the swing arm 163. The end of the swing arm 163 is provided with a driving gear 165 that rotates with it. A support rod 166 is fixed to the support rod 164. A driven gear 167 is provided on the support rod 166. A linkage rod 168 is provided on one side of the driven gear 167. One end of the linkage rod 168 is fixed to the movable base plate 16, and the other end of the linkage rod 168 is provided with a rack 169. The driving gear 165 and the rack 169 respectively mesh with the driven gear 167. The end of the swing arm 163 and the driving gear 165 are keyed together.

[0034] This invention provides a separate noise-reducing and vibration-damping optical platform for patch-clamp experiments. Through the inclusion of a buoyancy tank 1, the experimental operating platform 3 can be separated from the base plate platform 2 used to house the equipment. The buoyancy generated by the fluid filling the buoyancy tank 1 provides floating support for the operating platform 3, avoiding direct and indirect contact between the operating platform 3 and the base plate platform 2. This also significantly reduces the energy transmitted from vibrations on the base plate platform 2 to the operating platform 3. Fluids such as hydraulic oil and silicone oil can be used in the buoyancy tank 1. This device can impede mechanical vibrations from inside and outside the laboratory, traffic vibrations, building vibrations, and mechanical vibrations generated by experimental equipment such as microscopes, micromanipulators, and pumps during operation. These vibrations are prevented from being transmitted through the base plate platform 2 or other experimental platforms to the patch-clamp experimental operating platform 3 or other operating devices, thus avoiding signal noise interference during the experiment. This maximizes the smooth operation of the patch-clamp experiment and solves the problem of signal noise affecting microcurrents during the use of the patch-clamp experimental operating platform.

[0035] Specifically, the outer shell of the buoyancy tank 1 is supported by the shock-absorbing column 21. First, the vibration reduction effect of the shock-absorbing column 21 absorbs and weakens the vibration transmitted on the base plate platform 2. Most of the weakened vibration energy is transmitted through the side wall of the buoyancy tank 1 to the fluid material in the cavity 12 formed inside the buoyancy tank 1. The vibration energy is absorbed by the fluid material, which largely avoids the transmission of vibration energy to the operating platform 3, and helps to improve the accuracy of the patch clamp experiment results.

[0036] On another front, some of the vibration energy is transmitted from the damping spring 22 to the movable base plate 16, causing the movable base plate 16 to vibrate. During the up / down vibration of the movable base plate 16, the swing arm 163 is first driven to swing through the bent rod 161 and the vertical rod 162. While the swing arm 163 swings, the active gear 165 rotates accordingly, which in turn drives the rack 169 to rise / fall through the rotation of the driven gear 167. Finally, the sealing plate 15 is driven to rise / fall through the linkage rod 168 to draw / discharge the fluid in the compartment 12, which helps to reduce the change in liquid level in the first hole 13 and reduce the floating amplitude of the float plate 32 and the floating base column 31 when vibrated.

[0037] Based on the above embodiments, more specifically, the first hole 13 is located in the middle of the partition 11, and the second hole 14 is located on the annular path surrounding the first hole 13. The area of ​​the movable base plate 16 is S, the area of ​​the first hole 13 is S1, the area of ​​the second hole 14 is S2, and the transmission ratio of the driving gear 165 and the driven gear 167 is S2:S, so that the fluid volume change in the compartment 12 is consistent with the fluid volume change in the second hole 14.

[0038] In this embodiment, the sub-cavity 12, the first hole 13, and the second hole 14 are all in a vacuum state. Under normal circumstances, changes in the fluid volume in the sub-cavity 12 will directly affect the position of the float plate 32 and the sealing plate 15. In order to avoid changes in the liquid level in the first hole 13 and keep the float plate 32 in a relatively stable state, this embodiment adopts a linkage between the movable base plate 16 and the sealing plate 15. During the vibration process, the movable base plate 16 can drive the sealing plate 15 to move, so that the fluid in the sub-cavity 12 can directly enter / flow out of the second hole 14, while the fluid volume remaining in the first hole 13 is not affected, thus stabilizing the position of the float plate 32.

[0039] Specifically, if S:S1:S2 = 10:4:1, the vibration of the movable base plate 16 causes the liquid level in the compartment 12 to rise by h, then the volume of fluid entering the first hole 13 and the second hole 14 is 10h. According to the principle of communicating vessels, under normal circumstances, the liquid level rise in the first hole 13 and the second hole 14 is h1 = 10h / (S1+S2), which gives h1 = 2. At this time, the sealing plate 15 and the float plate 32 move together. If the fluid with a volume of 10h only enters the second hole 14, then the liquid level rise in the second hole 14 should be h2 = 10h / S2, h2 = 10, which gives h:h2 = S2:S = 1:10. At this time, the sealing plate 15 moves, and the float plate 32 does not move. That is, the height of movement of the sealing plate 15 and the movable base plate 16 under linkage is inversely proportional to their area. Since the movable base plate 16 is linked with the driving gear 165 and the sealing plate 15 is linked with the driven gear 167, for every distance the movable base plate 16 moves, the sealing plate 15 moves 10 distances. Therefore, the transmission ratio of the driving gear 165 and the driven gear 167 should be 1:10. Thus, during the reciprocating cycle of the movable base plate 16 vibrating up and down, the liquid level in the compartment 12 can be fluctuated, with only the liquid flowing in and out of the second hole 14, while the liquid level in the first hole 13 remains basically unchanged.

[0040] In the above embodiments, because the second hole 14 located below the sealing plate 15 lacks a necessary limiting structure, the sealing plate 15 is prone to dislodging from the second hole 14 when moving downwards. To solve this problem, based on the preferred embodiment provided in the above embodiments, the present invention provides a limiting structure to restrict the range of motion of the sealing plate 15 when moving downwards, such as... Figure 5-6As shown, a limiting block 141 is provided on the inner wall of the second hole 14, and the limiting block 141 is located near the lower end of the second hole 14. A limiting groove 151 is formed on the sealing plate 15, located on the lower surface of the sealing plate 15. The limiting block 141 is positioned corresponding to the limiting groove 151 and is embedded therein. The limiting grooves 151 are equidistantly spaced circumferentially at the edge of the sealing plate 15. The number of limiting blocks 141 and limiting grooves 151 is at least two, and the number of limiting grooves 151 is not less than the number of limiting blocks 141. In this embodiment, the limiting structure makes it difficult for the sealing plate 15 to detach from the second hole 14, improving the stability of the sealing plate 15 during movement.

[0041] Based on the above embodiments, and more specifically, please continue to refer to... Figure 5-6 As shown, the upper surface of the sealing plate 15 is provided with a telescopic rod 33, which is located near the edge of the sealing plate 15. The telescopic rod 33 is used to keep the sealing plate 15 in a floating path in the vertical direction. One end of the telescopic rod 33 is fixedly connected to the sealing plate 15, and a limiting ring 18 is provided on the inner wall of the second hole 14. The other end of the telescopic rod 33 is located on the limiting ring 18.

[0042] In the above embodiments, the telescopic rod 33 can guide the movement of the sealing plate 15, preventing the sealing plate 15 from deflecting laterally during movement and causing uneven force on the inner wall of the second hole 14, which would affect the sealing performance between the two. It can also prevent the limiting groove 151 on the sealing plate 15 from having a mismatch with the limiting block 141, thus preventing the formation of a better fitting state.

[0043] In the above embodiments, because the telescopic rod 33 is located between the limiting ring 18 and the sealing plate 15, the telescopic rod 33 restricts the movable distance between the limiting ring 18 and the sealing plate 15. To solve the above problem, the present invention, based on the preferred embodiments provided in the above embodiments, such as... Figure 6 As shown, a connecting hole 17 is provided on the limiting ring 18. A sliding top block 171 and a limiting body 172 are provided in the connecting hole 17. The top block 171 is used to connect the telescopic rod 33. As the telescopic rod 33 is subjected to force, the top block 171 gradually slides upward and stops against the limiting body 172. The base of the telescopic rod 33 retracts into the connecting hole 17.

[0044] In the above embodiment, the top surface of the floating base column 31 is higher than the top edge of the buoyancy tank 1, so that when the operating platform 3 is fixedly connected to the floating base column 31, the operating platform 3 and the buoyancy tank 1 are in a separate state, thus avoiding the transmission of vibration from the buoyancy tank 1 to the operating platform 3.

[0045] In the above embodiments, in order to ensure the volume of fluid substances contained in the cavity 12, the partition 11 is preferably located at the middle height of the inner cavity of the buoyancy tank 1.

[0046] In the above embodiments, the rigid connection between the shock-absorbing column 21 and the buoyancy tank 1 makes it easier to transmit vibrations to the bottom of the buoyancy tank 1, thus increasing the amount of vibration absorbed by the fluid material inside. To solve the above problem, such as Figure 3-4 As shown, in the preferred embodiment provided by the above embodiments of the present invention, a shock-absorbing ring 23 is sleeved on the shock-absorbing column 21. The shock-absorbing ring 23 is disposed near the bottom of the shock-absorbing column 21 and is in contact with the upper surface of the substrate platform 2 to reduce the vibration transmitted from the substrate platform 2 to the shock-absorbing column 21. In this embodiment, when the shock-absorbing column 21 is made of a rigid material to ensure its load-bearing capacity, the shock-absorbing ring 23 is preferably made of a damping material, such as rubber, foam, or a rubber-metal composite material, thereby reducing the vibration frequency and intensity on the shock-absorbing column 21.

[0047] The above embodiments are merely exemplary embodiments of this application and are not intended to limit this application. The scope of protection of this application is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this application within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this application.

Claims

1. A separate noise reduction and vibration damping optical platform for patch-clamp experiments, comprising: A buoyancy tank (1) has a partition (11) fixed inside it. The partition (11) is arranged along the cross-section of the buoyancy tank (1). The partition (11) divides the inner cavity of the buoyancy tank (1) into a sub-cavity (12) located below the partition (11). The sub-cavity (12) is used to fill fluid. A first hole (13) and a second hole (14) are provided through the partition (11). A movable sealing plate (15) is provided in the second hole (14). The fluid in the sub-cavity (12) can be forced into the first hole (13) and the second hole (14) by external pressure. A movable bottom plate (16) is provided at the inner bottom of the buoyancy tank (1). The circumferential side of the movable bottom plate (16) forms a seal with the inner wall of the buoyancy tank (1). The base plate platform (2) is supported and fixed on the base plate platform (2) by the outer bottom of the buoyancy tank (1) through the shock-absorbing column (21). The base plate platform (2) is provided with a damping spring (22) that penetrates the bottom of the buoyancy tank (1). The damping spring (22) supports the movable bottom plate (16) to separate from the inner bottom of the buoyancy tank (1). The vibration of the base plate platform (2) is weakened by the shock-absorbing column (21) and then transmitted to the buoyancy tank (1) and absorbed by the fluid in the cavity (12). The operating platform (3) is located above the buoyancy tank (1) and separated from it. A floating base column (31) is fixedly connected to the lower part of the operating platform (3). A floating plate (32) is fixedly connected to the lower part of the floating base column (31). The floating plate (32) closes the first hole (13) and is movably located inside it. The feature is that a bent rod (161) is fixedly provided on the bottom side of the movable base plate (16), a vertical rod (162) is hinged to one end of the bent rod (161), a swing arm (163) is hinged to one end of the vertical rod (162), a support rod (164) is provided on the outer wall of the buoyancy tank (1), the support rod (164) is axially connected to one end of the swing arm (163), and the end of the swing arm (163) is provided with an active gear (165) that rotates with it. A second support rod (166) is fixedly mounted on the first support rod (164). A driven tooth (167) is provided on the second support rod (166). A linkage rod (168) is provided on one side of the driven tooth (167). One end of the linkage rod (168) is fixedly connected to the movable base plate (16). The other end of the linkage rod (168) is provided with a rack (169). The driving tooth (165) and the rack (169) respectively mesh with the driven tooth (167).

2. The split-type noise reduction and vibration damping optical platform for patch-clamp experiments according to claim 1, characterized in that, The first hole (13) is located in the middle of the partition (11), and the second hole (14) is located on the annular path around the first hole (13).

3. The split-type noise reduction and vibration damping optical platform for patch-clamp experiments according to claim 2, characterized in that, The area of ​​the movable base plate (16) is S, the area of ​​the first hole (13) is S1, the area of ​​the second hole (14) is S2, and the transmission ratio of the driving tooth (165) and the driven tooth (167) is S2:S, so that the fluid volume change in the cavity (12) is the same as the fluid volume change in the second hole (14).

4. The split-type noise reduction and vibration damping optical platform for patch-clamp experiments according to claim 3, characterized in that, The end of the swing arm (163) and the drive tooth (165) are keyed together.

5. The split-type noise reduction and vibration damping optical platform for patch-clamp experiments according to claim 4, characterized in that, A limiting block (141) is provided on the inner wall of the second hole (14), and the limiting block (141) is located near the lower port of the second hole (14); The sealing plate (15) has a limiting groove (151) on it. The limiting groove (151) is located on the lower surface of the sealing plate (15). The limiting block (141) is positioned corresponding to the limiting groove (151) and is embedded therein. The limiting slots (151) are circumferentially equidistantly opened at the edge of the sealing plate (15). The number of limiting blocks (141) and limiting slots (151) is at least 2, and the number of limiting slots (151) is not less than the number of limiting blocks (141).

6. The split-type noise reduction and vibration damping optical platform for patch-clamp experiments according to claim 5, characterized in that, The upper surface of the sealing plate (15) is provided with a telescopic rod (33), which is located near the edge of the sealing plate (15). The telescopic rod (33) is used to keep the sealing plate (15) in a floating path in the vertical direction. One end of the telescopic rod (33) is fixedly connected to the sealing plate (15), and a limiting ring (18) is provided on the inner wall of the second hole (14). The other end of the telescopic rod (33) is located on the limiting ring (18).

7. The split-type noise reduction and vibration damping optical platform for patch-clamp experiments according to claim 6, characterized in that, The limiting ring (18) has a connecting hole (17), and a sliding top block (171) and a limiting body (172) are provided in the connecting hole (17). The top block (171) is used to connect the telescopic rod (33). The top block (171) gradually slides upward and stops against the limiting body (172) as the telescopic rod (33) is subjected to force. The base of the telescopic rod (33) retracts into the connecting hole (17).

8. The split-type noise reduction and vibration damping optical platform for patch-clamp experiments according to claim 7, characterized in that, The top surface of the floating base column (31) is higher than the top edge of the buoyancy tank (1) so that the operating platform (3) is separated from the buoyancy tank (1).

9. A separate noise reduction and vibration damping optical platform for patch-clamp experiments according to claim 8, characterized in that, The partition (11) is located at the middle height of the inner cavity of the buoyancy tank (1).

10. A separate noise reduction and vibration damping optical platform for patch-clamp experiments according to claim 9, characterized in that, A shock-absorbing ring (23) is fitted on the shock-absorbing column (21). The shock-absorbing ring (23) is located near the bottom of the shock-absorbing column (21) and is in contact with the upper surface of the substrate platform (2) to reduce the vibration transmitted from the substrate platform (2) to the shock-absorbing column (21).

Images