Beef cattle four-stage stomach regulating and intestine strengthening double-effect combined feeding method

Abstract

The invention belongs to the technical field of beef cattle feeding, and particularly relates to a four-stage stomach-regulating and intestine-strengthening double-effect combined feeding method for beef cattle. The feeding method comprises the following steps: S1, breeding calves: feeding the calves with breast milk three times a day and 3-6 kg of breast milk a day within 40 days after the calves are born; from the 41st day, the feed A is freely eaten, and the daily feed intake is 4-4.3% of the body weight; s2, in a skeleton period, when the beef cattle grow to 230-280 kg, the beef cattle freely eat feed A, and the daily feed intake is 4.0-4.3% of the weight of the beef cattle; s3, in the early stage of fattening, when the weight of the beef cattle is 350-400 kilograms, the beef cattle freely eat feed B, and the daily feed intake is 4.5-4.7% of the weight of the beef cattle; s4, in the middle and later stages of fattening, when the weight of the beef cattle exceeds 500 kg, the beef cattle freely eat the feed A, and the daily feed intake is 4.8-5.0% of the weight of the beef cattle. According to the feeding method, meat quality can be improved, the fattening period is shortened, and the income of farmers is increased.

Description

Technical Field

[0001] The invention belongs to the technical field of beef cattle breeding, and in particular relates to a beef cattle breeding method with a four-stage stomach-regulating and intestine-strengthening dual-effect combination. Background Art

[0002] Beef cattle refer to cattle that are raised specifically for the production of beef. Their main purpose is to produce high-quality and high-yield beef through efficient feeding and management. Beef cattle breeding is an important part of the global animal husbandry industry. It not only provides an important source of protein for humans, but also occupies an important position in the agricultural economy. Angus cattle: Originated in Scotland, it is one of the most common beef cattle breeds. Angus cattle are known for their black or red coat color, good muscle development and fat distribution, tender and juicy meat, and are suitable for producing high-grade beef. At present, there are few feeding methods specifically for Angus cattle.

[0003] Alfalfa hay is a high-quality roughage commonly used in beef cattle feed. It is rich in protein, vitamins and minerals, and is especially suitable for calves and beef cattle in the fattening period. However, despite its many advantages, alfalfa hay also has some potential disadvantages in practical applications. Alfalfa hay contains more cellulose. Although fiber helps maintain the intestinal health of beef cattle, it may also reduce the digestibility of feed and affect the absorption of other nutrients. The cellulose in alfalfa hay will produce a large amount of gas when fermented in the rumen, which may cause problems such as bloating and belching in beef cattle.

[0004] Chinese patent CN201510348250.9 provides a feed for grassless feeding of beef cattle and a preparation method thereof. The quality of Angus beef fed with the feed is not ideal and the feed conversion rate is not high.

[0005] Therefore, a feeding method specifically for Angus cattle without alfalfa hay was continued. Summary of the invention

[0006] The purpose of the invention is to provide a four-stage stomach-regulating and intestine-strengthening dual-effect combined feeding method for beef cattle.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] A beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method comprises the following steps:

[0009] S1. Calf training: Calf is fed with breast milk three times a day within 40 days after birth, 3-6 kg per day; starting from the 41st day, calf can freely eat feed A, and the daily feed intake is 4-4.3% of body weight;

[0010] S2, skeleton stage: when beef cattle grow to 230-280 kg, they can freely eat feed A, and the daily feed intake is 4.0-4.3% of body weight;

[0011] S3, early fattening period: When the weight of beef cattle is 350-400 kg, they can freely eat feed B, and the daily feed intake is 4.5-4.7% of their body weight;

[0012] The feed B comprises the following raw materials in parts by weight: 22-27 parts of corn, 14-19 parts of corn germ cake, 12-18 parts of soybean meal, 3-8 parts of bran, 0.5-0.9 parts of calcium carbonate, 0.4-0.9 parts of compound bacterial agent B, 0.3-0.5 parts of compound enzyme B, 1.8-2.2 parts of plant extract, 0.3-0.5 parts of calcium bicarbonate, and 0.1-0.2 parts of salt;

[0013] S4. Mid-to-late fattening period: When the weight of beef cattle exceeds 500 kg, they can freely eat feed A, and the daily feed intake is 4.8-5.0% of their body weight.

[0014] Furthermore, the feed A includes the following raw materials in parts by weight: 15-20 parts of corn, 17-22 parts of corn germ cake, 15-20 parts of palm meal, 13-18 parts of bran, 1.0-1.2 parts of compound bacterial agent A, 0.7-0.9 parts of compound enzyme A, 1.2-1.5 parts of plant extract, 0.7-1.2 parts of calcium bicarbonate, and 0.1-0.2 parts of salt.

[0015] Furthermore, the composite bacterial agent A comprises Bifidobacterium longum, Lactobacillus plantarum and Lactobacillus casei in a weight ratio of 1:(1.2-1.4):(0.5-0.8).

[0016] Furthermore, the composite bacterial agent B comprises Lactobacillus bulgaricus, Lactobacillus helveticus and Lactobacillus acidophilus in a weight ratio of (0.2-0.5):1:(1.6-1.8).

[0017] Furthermore, the complex enzyme A comprises cellulase, bromelain and ficin in a weight ratio of (1.5-1.7):1:(0.6-0.8).

[0018] Furthermore, the complex enzyme B comprises α-amylase, neutral protease and papain in a weight ratio of (1.5-1.7):(0.6-0.8):1.

[0019] The present invention can improve the feed conversion rate of Angus cattle and increase the meat yield by adding specific composite bacteria and composite enzymes to feed A and feed B and feeding them respectively at different stages. Angus cattle are ruminants, and their rumen is a key part of the digestive system, which is responsible for decomposing complex plant feed into absorbable small molecule nutrients. Adding specific composite bacteria at different feeding stages can enhance the microbial activity in the rumen, thereby improving the digestibility of feed. Adding composite bacteria and composite enzymes at the same time can further improve the digestibility and utilization rate of feed, ensuring that beef cattle maintain an efficient growth rate and high-quality meat quality.

[0020] Furthermore, the preparation method of the plant extract comprises the following steps:

[0021] (1) Mixing Cassia Seed, Ligustrum Lucidum Fruit, Saposhnikovia Divaricata and Eucommia Leaf, and crushing to less than 100 meshes to obtain a mixed material;

[0022] (2) The mixed material and water are mixed in a weight ratio of 1:(8-10) to obtain a fermentation liquid, and Lactobacillus plantarum is added to the fermentation liquid. The amount of Lactobacillus plantarum is 10 6 -10 7 CFU / mL fermentation liquid; ferment at 38-40℃ for 6-8h, sterilize, and continue to add Lactobacillus casei, the dosage of Lactobacillus casei is 10 8 -10 9 CFU / mL fermentation liquid, ferment at 38-40℃ for 3-5h, sterilize, and finally add Lactobacillus helveticus. The dosage of Lactobacillus helveticus is 10 7 -10 8 CFU / mL fermentation liquid, ferment at 38-40°C for 4-6h, sterilize to obtain fermentation liquid;

[0023] (3) The fermentation broth was filtered using a 0.45 μm filter membrane, and the filtrate was concentrated and freeze-dried to obtain a plant extract.

[0024] Furthermore, the weight ratio of Cassia seed, Ligustrum lucidum fruit, Saposhnikovia divaricata and Eucommia ulmoides leaf is 1:(1.2-1.4):(0.4-0.7):(1.6-1.9).

[0025] The present invention can increase the content of unsaturated fatty acids in beef by adding plant extracts to feed A and feed B. The polyphenol compounds in Cassia seed have strong antioxidant capacity, can remove free radicals, protect cells from oxidative damage, reduce lipid peroxidation, and thus are conducive to healthy fatty acid synthesis. Oleanolic acid and ursolic acid in Ligustrum lucidum can promote the synthesis of unsaturated fatty acids by activating peroxisome proliferator-activated receptors (PPARs), especially PPAR-α and PPAR-γ, while inhibiting the β-oxidation of fatty acids and reducing the accumulation of saturated fatty acids. The coumarin compounds in Saposhnikovia divaricata have anti-inflammatory effects, can reduce the negative effects of inflammatory reactions on fat metabolism, and promote healthy fatty acid synthesis. Eucommia ulmoides leaves can also enhance the immunity of beef cattle, reduce the occurrence of diseases, ensure their healthy growth, and further improve the synthesis efficiency of fatty acids. The active components in the four plants work synergistically, and the active components in the plant extracts work through multiple mechanisms, including promoting the synthesis of unsaturated fatty acids, inhibiting the accumulation of saturated fatty acids, improving intestinal health and nutrient absorption, etc., which can significantly increase the content of unsaturated fatty acids in beef.

[0026] Furthermore, the preparation method of the feed A is as follows: the raw materials of the feed A are mixed evenly, and crushed until all the raw materials pass through a sieve with a particle size of 5-7 mm.

[0027] Furthermore, the preparation method of the feed B is: the raw materials of the feed B are mixed evenly, and crushed until all the particles pass through a sieve with a particle size of 5-7 mm.

[0028] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0029] 1. The present invention can improve the feed conversion rate of Angus cattle and increase the meat yield by adding specific composite bacteria and composite enzymes to feed A and feed B and feeding them at different stages.

[0030] 2. The present invention can increase the content of unsaturated fatty acids in beef by adding plant extracts to feed A and feed B. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] The raw materials used in the following examples of the present invention are all commercially available products:

[0033] Cellulase, enzyme activity 50u / mg, Shanghai Yuanye Biotechnology Co., Ltd.

[0034] Neutral protease, enzyme activity 100u / mg, Shanghai Yuanye Biotechnology Co., Ltd.

[0035] α-Amylase, enzyme activity 200u / mg, Shanghai Yuanye Biotechnology Co., Ltd.

[0036] Bromelain, enzyme activity 300u / mg, Shanghai Yuanye Biotechnology Co., Ltd.

[0037] Fig proteinase, enzyme activity 300u / mg, Shanghai Yuanye Biotechnology Co., Ltd.

[0038] Papain, enzyme activity 300u / mg, Shanghai Yuanye Biotechnology Co., Ltd.

[0039] Bifidobacterium longum, deposit number: CCTCCHB20082718, deposited in China Center for Type Culture Collection.

[0040] Lactobacillus plantarum, deposit number: CCTCCAB2010210, deposited in China Center for Type Culture Collection.

[0041] Lactobacillus casei, accession number: SHBCCD24737, deposited in Shanghai Microbiological Collection Center.

[0042] Lactobacillus bulgaricus, deposit number: CCTCCCB20082295, deposited in China Center for Type Culture Collection.

[0043] Lactobacillus helveticus, deposit number: CCTCCAB2010205, deposited in China Center for Type Culture Collection.

[0044] Lactobacillus acidophilus, deposit number: CCTCCAB2010208. Deposited in China Center for Type Culture Collection.

[0045] Example 1

[0046] This embodiment provides a four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method for beef cattle, comprising the following steps:

[0047] S1. Calf training: calves are fed breast milk three times a day for 40 days after birth, 4.5 kg per day; starting from the 41st day, they are allowed to eat feed A freely, with a daily feed intake of 4.2% of their body weight;

[0048] S2, skeleton stage: when the beef cattle grow to 250 kg, they can freely eat feed A, and the daily feed intake is 4.1% of body weight;

[0049] S3, early fattening period: When the weight of beef cattle is 370 kg, they can freely eat feed B, and the daily feed intake is 4.6% of their body weight;

[0050] S4. Mid-to-late fattening period: When the weight of beef cattle exceeds 500 kg, they can freely eat feed A, and the daily feed intake is 4.9% of their body weight.

[0051] The feed A comprises the following raw materials in parts by weight: 18 parts of corn, 20 parts of corn germ cake, 17 parts of palm meal, 15 parts of bran, 1.1 parts of compound bacterial agent A, 0.8 parts of compound enzyme A, 1.3 parts of plant extract, 1 part of calcium bicarbonate, and 0.15 parts of salt. The preparation method of the feed A is as follows: the raw materials of the feed A are mixed evenly, and crushed until all of them pass through a sieve with a particle size of 6 mm.

[0052] The composite bacterial agent A comprises bifidobacterium longum, lactobacillus plantarum and lactobacillus casei in a weight ratio of 1:1.3:0.6. The composite enzyme A comprises cellulase, bromelain and ficin in a weight ratio of 1.6:1:0.7.

[0053] The feed B comprises the following raw materials in parts by weight: 25 parts of corn, 16 parts of corn germ cake, 15 parts of soybean meal, 6 parts of bran, 0.7 parts of calcium carbonate, 0.5 parts of compound bacterial agent B, 0.4 parts of compound enzyme B, 2 parts of plant extract, 0.4 parts of calcium bicarbonate, and 0.15 parts of salt. The preparation method of the feed B is: the raw materials of the feed B are mixed evenly, and crushed until all of them pass through a sieve with a particle size of 6 mm.

[0054] The composite bacterial agent B comprises Lactobacillus bulgaricus, Lactobacillus helveticus and Lactobacillus acidophilus in a weight ratio of 0.3:1:1.7. The composite enzyme B comprises alpha-amylase, neutral protease and papain in a weight ratio of 1.6:0.7:1.

[0055] The preparation method of the plant extract comprises the following steps:

[0056] (1) Mixing Cassia seed, Ligustrum lucidum fruit, Saposhnikovia divaricata and Eucommia ulmoides leaf in a weight ratio of 1:1.3:0.5:1.7, and crushing the mixture to less than 100 meshes to obtain a mixture;

[0057] (2) The mixed material and water were mixed in a weight ratio of 1:9 to obtain a fermentation liquid, and Lactobacillus plantarum was added to the fermentation liquid. The amount of Lactobacillus plantarum was 10 6 CFU / mL fermentation liquid; ferment at 39℃ for 7h, sterilize, and continue to add Lactobacillus casei, the amount of Lactobacillus casei is 10 9 CFU / mL fermentation liquid, fermented at 39℃ for 4h, sterilized, and finally added with Lactobacillus helveticus. The amount of Lactobacillus helveticus was 10 8 CFU / mL fermentation broth, ferment at 39°C for 5 h, sterilize, and obtain fermentation broth;

[0058] (3) The fermentation broth was filtered using a 0.45 μm filter membrane, and the filtrate was concentrated and freeze-dried to obtain a plant extract.

[0059] Example 2

[0060] This embodiment provides a four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method for beef cattle, comprising the following steps:

[0061] S1. Calf training: calves are fed with breast milk three times a day for 40 days after birth, 5 kg per day; starting from the 41st day, they are allowed to eat feed A freely, with a daily feed intake of 4% of their body weight;

[0062] S2, skeleton stage: when the beef cattle grow to 230 kg, they can freely eat feed A, and the daily feed intake is 4.3% of their body weight;

[0063] S3, early fattening period: When the weight of beef cattle is 350 kg, they can freely eat feed B, and the daily feed intake is 4.7% of their body weight;

[0064] S4. Mid-to-late fattening period: When the weight of beef cattle exceeds 500 kg, they can freely eat feed A, and the daily feed intake is 4.8% of their body weight.

[0065] The feed A comprises the following raw materials in parts by weight: 20 parts of corn, 17 parts of corn germ cake, 15 parts of palm meal, 18 parts of bran, 1.0 parts of compound bacterial agent A, 0.9 parts of compound enzyme A, 1.5 parts of plant extract, 0.7 parts of calcium bicarbonate, and 0.2 parts of salt. The preparation method of the feed A is: the raw materials of the feed A are mixed evenly, and crushed until all of them pass through a sieve with a particle size of 6 mm.

[0066] The composite bacterial agent A comprises bifidobacterium longum, lactobacillus plantarum and lactobacillus casei in a weight ratio of 1:1.3:0.6. The composite enzyme A comprises cellulase, bromelain and ficin in a weight ratio of 1.6:1:0.7.

[0067] The feed B comprises the following raw materials in parts by weight: 22 parts of corn, 19 parts of corn germ cake, 12 parts of soybean meal, 8 parts of bran, 0.5 parts of calcium carbonate, 0.9 parts of compound bacterial agent B, 0.3 parts of compound enzyme B, 2.2 parts of plant extract, 0.3 parts of calcium bicarbonate, and 0.2 parts of salt. The preparation method of the feed B is: the raw materials of the feed B are mixed evenly, and crushed until all of them pass through a sieve with a particle size of 6 mm.

[0068] The composite bacterial agent B comprises Lactobacillus bulgaricus, Lactobacillus helveticus and Lactobacillus acidophilus in a weight ratio of 0.2:1:1.6. The composite enzyme B comprises alpha-amylase, neutral protease and papain in a weight ratio of 1.5:0.6:1.

[0069] The preparation method of the plant extract comprises the following steps:

[0070] (1) Mixing Cassia seed, Ligustrum lucidum fruit, Saposhnikovia divaricata and Eucommia ulmoides leaf in a weight ratio of 1:1.2:0.4:1.6, and crushing the mixture to less than 100 meshes to obtain a mixture;

[0071] (2) The mixed material and water were mixed in a weight ratio of 1:10 to obtain a fermentation liquid, and Lactobacillus plantarum was added to the fermentation liquid. The amount of Lactobacillus plantarum was 10 7 CFU / mL fermentation liquid; ferment at 40℃ for 8h, sterilize, and continue to add Lactobacillus casei, the amount of Lactobacillus casei is 10 9 CFU / mL fermentation liquid, fermented at 40℃ for 5h, sterilized, and finally added with Lactobacillus helveticus. The amount of Lactobacillus helveticus was 10 8 CFU / mL fermentation broth, ferment at 38°C for 6 h, sterilize, and obtain fermentation broth;

[0072] (3) The fermentation broth was filtered using a 0.45 μm filter membrane, and the filtrate was concentrated and freeze-dried to obtain a plant extract.

[0073] Comparative Example 1

[0074] The difference between this comparative example and Example 1 is that the feed A includes the following raw materials in parts by weight: 23 parts of corn, 15 parts of corn germ cakes, 12 parts of palm meal, 20 parts of bran, 0.8 parts of compound bacterial agent A, 1.3 parts of compound enzyme A, 1.1 parts of plant extract, 1 part of calcium bicarbonate, and 0.15 parts of salt.

[0075] Comparative Example 2

[0076] The difference between this comparative example and Example 1 is that the composite bacterial agent A comprises Bifidobacterium longum, Lactobacillus plantarum and Lactobacillus casei in a weight ratio of 1:1:1.

[0077] Comparative Example 3

[0078] The difference between this comparative example and Example 1 is that the complex enzyme A comprises cellulase, bromelain and ficin in a weight ratio of 1:1:1.

[0079] Comparative Example 4

[0080] The difference between this comparative example and Example 1 is that the feed B includes the following raw materials in parts by weight: 20 parts of corn, 21 parts of corn germ cakes, 10 parts of soybean meal, 11 parts of bran, 0.7 parts of calcium carbonate, 1 part of compound bacterial agent B, 0.9 parts of compound enzyme B, 1 part of plant extract, 0.4 parts of calcium bicarbonate, and 0.15 parts of salt.

[0081] Comparative Example 5

[0082] The difference between this comparative example and Example 1 is that the composite bacterial agent B comprises Lactobacillus bulgaricus, Lactobacillus helveticus and Lactobacillus acidophilus in a weight ratio of 1:1:1.

[0083] Comparative Example 6

[0084] The difference between this comparative example and Example 1 is that the complex enzyme B comprises α-amylase, neutral protease and papain in a weight ratio of 1:1:1.

[0085] Comparative Example 7

[0086] The difference between this comparative example and Example 1 is that Cassia seed, Ligustrum lucidum fruit, Saposhnikovia divaricata and Eucommia ulmoides leaf in a weight ratio of 1:1:1:1 are mixed and crushed to less than 100 meshes to obtain a mixture.

[0087] Comparative Example 8

[0088] The difference between this comparative example and Example 1 is that the mixed material and water are mixed in a weight ratio of 1:9 to obtain a fermentation liquid, and Bifidobacterium longum is added to the fermentation liquid. The amount of Bifidobacterium longum is 10 6 CFU / mL fermentation liquid; ferment at 39℃ for 7h, sterilize, and continue to add Lactobacillus bulgaricus, the dosage of Lactobacillus bulgaricus is 10 9 CFU / mL fermentation liquid, ferment at 39℃ for 4h, sterilize, and finally add Lactobacillus acidophilus, the dosage of Lactobacillus acidophilus is 10 8 CFU / mL fermentation broth, ferment at 39°C for 5h, sterilize, and obtain fermentation broth.

[0089] Comparative Example 9

[0090] The difference between this comparative example and Example 1 is that the feed prepared in Example 1 in Chinese patent CN201510348250.9 is used instead of feed A and feed B of the present invention.

[0091] Performance Testing

[0092] Angus bulls were taken, 50 in each group, and fed using the feeding methods of Examples 1-2 and Comparative Examples 1-9 of the present invention for 5 months, and various indicators were counted. After the feeding, the fat content of the longissimus dorsi muscle of the beef cattle was measured, and the fat in the sample was extracted by reflux method (90° C., 90 min) and methylated, and the fatty acid content of the beef was measured by chromatography-mass spectrometry. The results are shown in Table 1.

[0093] Table 1 Performance test results

[0094]

[0095] From the above performance test results, it can be seen that the feeding methods of Examples 1-2 have high feed conversion rates, high unsaturated fatty acid content in beef, and good beef quality, especially the comprehensive performance of Example 1 is the most outstanding.

[0096] However, the comparative examples were significantly inferior to the embodiments in the corresponding performance tests because they did not adopt the necessary technical solutions. In comparative example 1, the ratio of feed A was changed, and in comparative example 4, the ratio of feed B was changed, the feed conversion rate was reduced, and the meat quality deteriorated. In comparative example 2, the ratio of composite bacterial agent A was changed, and the ratio of composite enzyme A was changed in comparative example 3. In comparative example 5, the ratio of composite bacterial agent B was changed, and in comparative example 6, the ratio of composite enzyme B was changed. The feed conversion rate of Angus cattle was reduced, and the meat yield was reduced. In comparative example 7, the ratio of Cassia seed, Ligustrum lucidum, Saposhnikovia divaricata and Eucommia ulmoides leaves was changed. In comparative example 8, different bacteria were used for plant extracts, and the content of unsaturated fatty acids in beef decreased. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effect.

[0097] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method for beef cattle, characterized in that: The following steps are involved: S1. Calf training: Calf is fed with breast milk three times a day within 40 days after birth, 3-6 kg per day; starting from the 41st day, calf can freely eat feed A, and the daily feed intake is 4-4.3% of body weight; S2, skeleton stage: when beef cattle grow to 230-280 kg, they can freely eat feed A, and the daily feed intake is 4.0-4.3% of body weight; S3, early fattening period: When the weight of beef cattle is 350-400 kg, they can freely eat feed B, and the daily feed intake is 4.5-4.7% of their body weight; The feed B comprises the following raw materials in parts by weight: 22-27 parts of corn, 14-19 parts of corn germ cake, 12-18 parts of soybean meal, 3-8 parts of bran, 0.5-0.9 parts of calcium carbonate, 0.4-0.9 parts of compound bacterial agent B, 0.3-0.5 parts of compound enzyme B, 1.8-2.2 parts of plant extract, 0.3-0.5 parts of calcium bicarbonate, and 0.1-0.2 parts of salt; S4. Mid-to-late fattening period: When the weight of beef cattle exceeds 500 kg, they can freely eat feed A, and the daily feed intake is 4.8-5.0% of their body weight.

2. The beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method according to claim 1, characterized in that: The feed A comprises the following raw materials in parts by weight: 15-20 parts of corn, 17-22 parts of corn germ cake, 15-20 parts of palm meal, 13-18 parts of bran, 1.0-1.2 parts of compound bacterial agent A, 0.7-0.9 parts of compound enzyme A, 1.2-1.5 parts of plant extract, 0.7-1.2 parts of calcium bicarbonate, and 0.1-0.2 parts of salt.

3. The beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method according to claim 2, characterized in that: The composite bacterial agent A comprises Bifidobacterium longum, Lactobacillus plantarum and Lactobacillus casei in a weight ratio of 1:(1.2-1.4):(0.5-0.8).

4. The beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method according to claim 2, characterized in that: The composite bacterial agent B comprises Lactobacillus bulgaricus, Lactobacillus helveticus and Lactobacillus acidophilus in a weight ratio of (0.2-0.5):1:(1.6-1.8).

5. The beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method according to claim 2, characterized in that: The complex enzyme A comprises cellulase, bromelain and ficin in a weight ratio of (1.5-1.7):1:(0.6-0.8).

6. The beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method according to claim 2, characterized in that: The complex enzyme B comprises α-amylase, neutral protease and papain in a weight ratio of (1.5-1.7):(0.6-0.8):

1.

7. The beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method according to claim 2, characterized in that: The preparation method of the plant extract comprises the following steps: (1) Mixing Cassia Seed, Ligustrum Lucidum Fruit, Saposhnikovia Divaricata and Eucommia Leaf, and crushing to less than 100 meshes to obtain a mixed material; (2) Mix the mixture and water in a weight ratio of 1:(8-10) to obtain a fermentation liquid, and add animal Bifidobacterium to the fermentation liquid. The amount of animal Bifidobacterium is 10 6 -10 7 CFU / mL fermentation liquid; ferment at 38-40℃ for 6-8h, sterilize, and continue to add Lactobacillus casei, the dosage of Lactobacillus casei is 10 8 -10 9 CFU / mL fermentation liquid, ferment at 38-40℃ for 3-5h, sterilize, and finally add Bifidobacterium bifidum. The dosage of Bifidobacterium bifidum is 10 7 -10 8 CFU / mL fermentation liquid, ferment at 38-40°C for 4-6h, sterilize to obtain fermentation liquid; (3) The fermentation broth was filtered using a 0.45 μm filter membrane, and the filtrate was concentrated and freeze-dried to obtain a plant extract.

8. The beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method according to claim 7, characterized in that: The weight ratio of Cassia seed, Ligustrum lucidum fruit, Saposhnikovia divaricata and Eucommia ulmoides leaf is 1:(1.2-1.4):(0.4-0.7):(1.6-1.9).

9. The beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method according to claim 2, characterized in that: The preparation method of the feed A is as follows: the raw materials of the feed A are mixed evenly, and crushed until all the raw materials pass through a sieve with a particle size of 5-7 mm.

10. The beef cattle four-stage stomach-regulating and intestinal-strengthening dual-effect feeding method according to claim 1, characterized in that: The preparation method of the feed B is as follows: the raw materials of the feed B are mixed evenly, and crushed until all the raw materials pass through a sieve with a particle size of 5-7 mm.

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