Pesticide composition for preventing and treating grape thrips

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating grape thrips. The pesticide composition for preventing and treating the grape thrips is an insecticidal composition, and the effective component of the insecticidal composition is formed by compounding methoxypiperidine ethyl ester and epoxy thiacloprid or rotenone; wherein the mass ratio of the methoxypiperidine ethyl ester to the epoxy thiacloprid is (1-8): (12-1); the mass ratio of the methoxypiperidine ethyl ester to the rotenone is (1-20): (20-1). According to the invention, methoxypiperidine ethyl ester is compounded with epoxy thiacloprid or rotenone, and an indoor activity test shows that the compounded composition has a synergistic effect on the tobacco thrips of grapes, can improve the prevention and treatment effect on the tobacco thrips of grapes, and can reduce the risk of drug resistance and delay the generation and development of the drug resistance of the tobacco thrips compared with a single agent; and a support is provided for treating the drug resistance of the tobacco thrips and guaranteeing the safe production of grapes.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide control, and particularly relates to a pesticide composition for controlling grape thrips. Background Art

[0002] Grapes are one of the most common fruits on the market. They are highly praised for their diverse varieties, high nutritional value and economic value. In recent years, with the expansion of grape planting, pests and diseases have become increasingly prominent. Tobacco thrips (Thripstabaci Lindeman) is one of the important pests that harm grapes. It belongs to the family Thripidae of the order Thysanoptera and sucks the leaves, flowers, stems and fruits of plants with its rasping mouthparts. In grape-growing areas, tobacco thrips suck the juice of grape berries, especially unripe berries. The surface of the sucked berries may show symptoms such as depressions and black spots, and sometimes perforations, which affect the yield and quality of the fruit. In addition, tobacco thrips can also spread viruses, such as the common tobacco streak virus TSV, iris yellow spot virus IYSV and tomato spotted wilt virus TSWV, which further harm grapes.

[0003] At present, insecticides are mainly used to control tobacco thrips in grape production. However, since tobacco thrips widely harm more than 150 plants such as onions, cotton, grapes and lilies, they are subject to great selection pressure from various pesticides and develop resistance rapidly. Studies have reported that tobacco thrips have developed resistance to a variety of existing pesticides such as pyrethroids, carbamates, organophosphates and spinosad. With the accumulation of resistance year by year, the effectiveness of pesticides will decrease year by year, and even become ineffective. Therefore, it is of great significance to evaluate and screen new pesticides to control the resistance of tobacco thrips and ensure the safe production of grapes.

[0004] Spiropydine ethyl ester (Spiropidion, CAS: 1229023-00-0) is a spirocyclic quaternary ketoacid insecticide developed by Syngenta. It is the fourth spirocyclic quaternary ketoacid insecticide after Bayer's spirodiclofen, spiromesiclofen and spirotetramat. Spiropydine ethyl ester is a lipid biosynthesis inhibitor. It mainly inhibits the activity of ACCase in the process of fat synthesis in the pest body, destroys the synthesis of lipids, thereby blocking the normal energy metabolism of the pest, and ultimately leading to the death of the pest. In addition, Spiropydine ethyl ester not only has a unique chemical structure and a novel mode of action, but also has the properties of bidirectional and systemic conduction. It can act on the larval stage and destroy the egg laying of adults. While reducing the insect population base, it inhibits the hatching of eggs that have been produced in plant tissues or on the surface of pests, and plays a strong insecticidal role. It can also be used as a resistance management tool. Although Spiropydine ethyl ester has the above advantages, as a single insecticidal active ingredient, it has limited action sites. If it is used as a single agent for a long time, there is a high risk of drug resistance. Summary of the invention

[0005] The object of the present invention is to provide a pesticide composition for controlling grape thrips, which comprises methylphenidate and existing insecticides for compound screening, exhibits a synergistic effect, can improve the control effect on grape tobacco thrips, and can reduce the risk of drug resistance of a single agent.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A pesticide composition for controlling grape thrips is an insecticidal composition, wherein the active ingredients of the insecticidal composition are compounded with methylphenidate and cyclohexaprid or rotenone; wherein the mass ratio of methylphenidate to cyclohexaprid is 1-8:12-1; and the mass ratio of methylphenidate to rotenone is 1-20:20-1.

[0008] A pesticide comprises the insecticide composition.

[0009] Preferably, the mass of the insecticide composition accounts for 0.5-75% of the total mass of the insecticide, and the remainder is auxiliary ingredients allowed to be added to the pesticide.

[0010] Preferably, the mass of the insecticide composition accounts for 5-45% of the total mass of the insecticide, and the remainder is auxiliary ingredients allowed to be added to the pesticide.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] The methoxyphenidine ethyl ester of the present invention is compounded with cyclohexaprid or rotenone. Through indoor activity tests, it is found that within the compounding mass ratio range of 1-8:12-1, the compounding of methoxyphenidine ethyl ester and cyclohexaprid has a synergistic effect on grape tobacco thrips; within the compounding mass ratio range of 1-20:20-1, the compounding of methoxyphenidine ethyl ester and rotenone has a synergistic effect on grape tobacco thrips. It is shown that when methoxyphenidine ethyl ester is compounded with cyclohexaprid or rotenone, the control effect on grape tobacco thrips can be improved, and compared with a single agent, the risk of drug resistance can be reduced, the generation and development of drug resistance of tobacco thrips can be delayed, and support can be provided for drug resistance management of tobacco thrips and ensuring safe production of grapes. DETAILED DESCRIPTION

[0013] The following is a clear and complete description of the technical solution of the patent of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0014] Example: Indoor toxicity assay

[0015] 1. Test reagents

[0016] 95% methoxyphenoxazine ethyl ester technical, 95% cyclohexidine technical, 95% rotenone technical

[0017] The original drug was dissolved in dimethyl sulfoxide, and then diluted with distilled water containing 0.01% Triton X-100 to prepare a high-concentration single-dose stock solution. Multiple groups of proportions were set up, and each single dose and each group of mixed proportions were diluted with distilled water containing 0.01% Triton X-100 in equal proportions to form 5 mass concentration gradients, with 50 mL of each mass concentration solution for standby use.

[0018] 2. Test pests

[0019] Nicotiana tabacum was collected from the grape planting base of Guangxi Academy of Agricultural Sciences and fed with fresh and clean young grape leaves. It was raised for four generations in an artificial climate chamber with a temperature of 25±1℃, a photoperiod of 16L / 8D, and a relative humidity of 70±5%. The second-instar nymphs of the same age were selected for toxicity determination test.

[0020] 3. Test methods

[0021] Take fresh and clean young grape leaves, cut out leaf discs with a diameter of 2 cm, immerse the leaf discs in the liquid for 10 seconds, take them out and dry them. Take a plastic centrifuge tube, poke several small holes at the bottom of the centrifuge tube and on the tube cover (the hole diameter is based on preventing the test pests from escaping), put the leaf discs into the plastic centrifuge tube, and put 3 leaf discs in each tube. Inoculate 15 test pests in each centrifuge tube, cover the tube cover and observe under the original conditions. Set 4 replicates for each mass concentration of the liquid, with 1 replicate per tube, and set the treatment containing 0.01% Triton X-100 distilled water as a blank control.

[0022] After 48 hours, the death of the test insects was investigated (the insects that could not crawl after being lightly touched with a brush tip were considered dead), and the corrected mortality rate of each treatment was calculated. The DPS software was used to perform regression analysis on the logarithmic value of the concentration of each treatment agent and the corrected mortality probability value to calculate the LC of each treatment agent. 50 .

[0023]

[0024] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0025]

[0026] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0027] 4. Pharmaceutical evaluation

[0028] The co-toxicity coefficient (CTC value) of the mixture was calculated according to the Sun Yunpei method, and the joint action classification standard was used: a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect; a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect; and a co-toxicity coefficient (CTC) 80 < 120 indicates an additive effect. The test results are shown in Table 1-2.

[0029] Table 1 The combined effect of methoxyfenapyr ethyl ester and cyclohexaprymide on grape tobacco thrips

[0030]

[0031] Table 2 The combined effect of methoxyphene ethyl ester and rotenone on grape tobacco thrips

[0032]

[0033] From the test results in Table 1 and Table 2, it can be seen that within the range of 1-8:12-1 compound mass ratio, the combination of methoxypiperidin ethyl ester and cyclohexaprid showed a synergistic effect on grape tobacco thrips; within the range of 1-20:20-1 compound mass ratio, the combination of methoxypiperidin ethyl ester and rotenone showed a synergistic effect on grape tobacco thrips.

[0034] In summary, when the methoxyphenidyl ethyl ester of the present invention is compounded with cyclohexaprid or rotenone, the control effect on grape tobacco thrips can be improved. Compared with a single agent, the risk of drug resistance can be reduced, the generation and development of drug resistance of tobacco thrips can be delayed, and support can be provided for drug resistance management of tobacco thrips and ensuring safe production of grapes.

Claims

1. A pesticide composition for controlling grape thrips, which is an insecticidal composition, characterized in that: The active ingredient of the insecticide composition is a compound of methoxyphenoxim ethyl ester and cyclohexaprid or rotenone; wherein, The mass ratio of ethyl methoxyphenecarb to cyclohexaprid is 1-8:12-1; The mass ratio of the methylphenidate to rotenone is 1-20:20-1.

2. An insecticide, characterized in that: The insecticide comprises the insecticide composition according to claim 1.

3. The insecticide according to claim 2, characterized in that The mass of the insecticide composition accounts for 0.5-75% of the total mass of the insecticide, and the remainder is the auxiliary components allowed to be added to the pesticide.

4. The insecticide according to claim 2, characterized in that The mass of the insecticide composition accounts for 5-45% of the total mass of the insecticide, and the remainder is auxiliary components allowed to be added to the pesticide.

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