Microemulsion composition as well as preparation and application thereof

Abstract

The invention provides a preparation method and application of microemulsion which is free of odor, capable of inhibiting and killing bacteria and high in butyrate content. The microemulsion comprises the following components in percentage by mass: 5%-40% of butyrate, 5%-35% of a surfactant, 2%-8% of a cosurfactant, 10%-30% of polyglycerol fatty acid ester and the balance of water. According to the invention, tributyrin which is insoluble in water and butyrate which is insoluble in oil form a microemulsion which is isotropic, good in stability and transparent under the action of the surfactant and the cosurfactant.

Description

Technical Field

[0001] The invention relates to the field of preparation of microemulsion compositions, and provides a method for preparing a microemulsion which is free of odor, capable of inhibiting and killing bacteria, and has a high content of butyrate, and its application in the fields of medicine, health products, food preservatives or feed. Background Art

[0002] After the implementation of the national policy of banning antibiotics in feed in 2020, the reduced use of antibiotics has led to a high incidence of bacterial diseases, especially diarrhea, ileitis and other problems. More and more products are being developed to replace antibiotics for use in animal production, among which butyrate and butyric acid glyceride are also relatively good alternatives to antibiotics.

[0003] Butyrate has the smell of rancid cheese and is somewhat appetizing. Its functions include: improving digestion and absorption capacity, enhancing immunity, inhibiting the growth of pathogenic microorganisms, replacing some antibiotics, promoting intestinal development, improving production performance, and preventing and treating diarrhea.

[0004] Butyric acid glyceride is the product of the esterification of butyric acid and glycerol. It is a short-chain fatty acid ester, a colorless oily liquid with a slight fatty aroma, a slightly bitter taste, and is extremely difficult to dissolve in water. Butyric acid glyceride has a long half-life, is safe and has no toxic side effects. It can be decomposed by pancreatic lipase in the animal intestine to produce butyric acid and glycerol, which are then transported by the blood to various tissues and organs of the body to exert their effects. The butyric acid and monobutyric acid glyceride produced by its decomposition can inhibit the growth of pathogenic microorganisms such as Salmonella, Escherichia coli, and Clostridium, and increase the number of beneficial bacteria such as Lactobacillus. Therefore, butyric acid glyceride has the following functions: (1) providing an energy source for intestinal mucosal cells, promoting the proliferation and differentiation of epithelial cells, increasing the height of intestinal villi, and promoting nutrient absorption; (2) enhancing intestinal barrier function by promoting the expression of tight junction proteins; (3) promoting the proliferation of beneficial intestinal microorganisms (such as lactic acid bacteria); (4) slowly releasing in the intestine and reaching the hind intestine; (5) improving animal production performance and serving as a substitute for antibiotic growth promoters.

[0005] At present, the products added by feed manufacturers and farmers are either butyrate or butyric acid glyceride alone. It is rare to find liquid products that mix butyrate and butyric acid glyceride together on the market. The reason is that butyrate is water-soluble, while butyric acid glyceride is fat-soluble. Simply mixing water-soluble substances and fat-soluble substances together will result in stratification, which will be uneven when added and inconvenient to use. Moreover, most of the products on the market are prepared into emulsions using emulsification processes, but emulsions are thermodynamically unstable and will stratify after being left for a period of time.

[0006] As the country's requirements for environmental protection become higher and higher, there are strict restrictions on the odorous substances emitted by feed production companies, which also makes companies reduce the addition of substances with strong odors. Butyrate powder has a rancid cheese smell and will have a certain odor during the use of feeding and production. On the one hand, there is pressure from the national environmental protection, and on the other hand, butyrate has a strong advantage in replacing antibiotics and improving production performance, which also brings troubles to feed production companies. Summary of the invention

[0007] Based on the above problems, the first aspect of the present invention provides a microemulsion composition, which comprises polyglycerol fatty acid ester and fatty acid salt; wherein the mass percentage of polyglycerol fatty acid ester and fatty acid salt is (5-35): (15-40).

[0008] In some embodiments, the molecular number of the polyglycerol in the polyglycerol fatty acid ester is 1-5, and the fatty acid includes butyric acid, caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid or linoleic acid, preferably butyric acid or lauric acid; in some embodiments, the polyglycerol fatty acid ester is one or a combination of monobutyrin, dibutyrin, tributyrin, monolaurin, dilaurin, and trilaurin.

[0009] In some embodiments, the fatty acid salt includes butyrate; in some embodiments, the butyrate includes one or a combination of sodium butyrate, calcium butyrate, potassium butyrate, and magnesium butyrate.

[0010] In some embodiments, the microemulsion composition further comprises a surfactant and a co-surfactant; in some embodiments, the surfactant is a Tween surfactant with an HLB value between 8 and 18 or a Span surfactant with an HLB value between 4.3 and 8.6; in some embodiments, the mass ratio of the Tween surfactant to the Span surfactant is 10:1-10; in some embodiments, the surfactant is Tween 20, Tween 40, Tween 60, Tween 80 or one or a combination of Span 20, Span 40, Span 60, and Span 80; in some embodiments, the co-surfactant is at least one of anhydrous ethanol, 95% ethanol, and n-butanol.

[0011] In some embodiments, the microemulsion composition contains the following mass percentages of each substance:

[0012] Butyrate 5%-35%, polyglycerol fatty acid ester 15%-40%, surfactant 5%-35%, co-surfactant 1%-8%, and the balance is water;

[0013] In some embodiments, the microemulsion composition contains the following mass percentages of each substance:

[0014] Butyrate 10%-30%, polyglycerol fatty acid ester 20%-35%, surfactant 10%-30%, co-surfactant 3%-6%, and the balance is water.

[0015] In some embodiments, the pH value of the microemulsion composition after being diluted 100-1000 times with water is 4-6.8; preferably 5-5.8.

[0016] The second aspect of the present invention provides a method for preparing a microemulsion composition, comprising the following steps:

[0017] Dissolve butyrate in water, adjust the pH value to 8-11, add a co-surfactant to obtain solution 1; mix polyglycerol fatty acid ester with the surfactant to obtain solution 2; mix solution 1 with solution 2 to obtain a microemulsion composition.

[0018] The third aspect of the present invention provides a use of a microemulsion composition in the preparation of antibacterial drugs, health products, food preservatives or feeds, wherein the microemulsion composition is derived from the microemulsion composition described in the first aspect or the microemulsion composition prepared by the method described in the second aspect.

[0019] In some embodiments, the medicine, health product, food preservative or feed is a medicine, health product, food preservative or feed for preventing, treating or assisting in treating bacterial infection;

[0020] In some embodiments, the bacteria are Gram-positive bacteria or Gram-negative bacteria; preferably Salmonella, Escherichia coli, Clostridium, Campylobacter, Enterococcus, Staphylococcus aureus.

[0021] The fourth aspect of the present invention provides a feed containing a microemulsion composition, wherein the microemulsion composition is the microemulsion composition of the first aspect or the microemulsion composition prepared by the method of the second aspect;

[0022] In some embodiments, the amount of the microemulsion composition added to the feed is 2-40 kg per 2 tons of feed; preferably 4-30 kg; more preferably 4-20 kg; more preferably 4-8 kg.

[0023] In some embodiments, the feed further contains premix and soybean oil; in some embodiments, the feed includes corn, soybean cake, wheat bran, fish meal, bone meal, shell meal, salt and vitamin mineral additives;

[0024] In some embodiments, the feed comprises the following weight percentages of each substance:

[0025] Corn 64.5%-68.5%, soybean cake 8%-12%, wheat bran 6%-10%, fish meal 1%-5%, bone meal 0.3%-2.3%, shell meal 0.5%-0.9%, salt 0.3%-0.7% and vitamin and mineral additives 3%-7%;

[0026] In some embodiments, the feed comprises the following weight percentages of each substance:

[0027] Corn 66.5%, soybean meal 10%, wheat bran 8%, fish meal 3%, bone meal 1.3%, shell meal 0.7%, salt 0.5% and vitamin and mineral additives 5%.

[0028] In some embodiments, the feed can be used to inhibit the growth of microorganisms; in some embodiments, the microorganisms can be bacteria; in some embodiments, the bacteria are not easy to survive at a pH of 5-6; in some embodiments, the bacteria are Gram-positive bacteria or Gram-negative bacteria; in some embodiments, the bacteria include Salmonella, Escherichia coli, Clostridium, Campylobacter, Enterococcus, Staphylococcus aureus.

[0029] Compared with the prior art, some embodiments of the present application have the following beneficial effects:

[0030] (1) The microemulsion prepared in some embodiments of the present application can form a uniform, stable, transparent, isotropic, and thermodynamically stable emulsified system, which can effectively avoid the problem of thermodynamic instability and stratification of the emulsion;

[0031] (2) The microemulsion prepared in some embodiments of the present application contains butyrate and polyglycerol fatty acid ester components, which can kill and inhibit bacteria and serve as a substitute for antibiotics.

[0032] (3) The microemulsion prepared by the present invention can be used in feed to not only effectively inhibit the reproduction of bacteria, but also effectively reduce the odor of butyrate during feed processing and reduce the odorous substances in the tail gas during feed processing. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The pictures are of the microemulsion of Example 1 before and after the centrifugation test, wherein Figure a is the microemulsion before the centrifugation test, and Figure b is the microemulsion after the centrifugation test;

[0034] Figure 2 The pictures are of the microemulsion of Example 2 before and after the centrifugation test, wherein Figure a is the microemulsion before the centrifugation test, and Figure b is the microemulsion after the centrifugation test;

[0035] Figure 3The pictures are of the microemulsion of Example 3 before and after the centrifugation test, wherein Figure a is the microemulsion before the centrifugation test, and Figure b is the microemulsion after the centrifugation test;

[0036] Figure 4 The pictures are of the microemulsion of Comparative Example 1 before and after the centrifugation test, wherein Figure a is the microemulsion before the centrifugation test, and Figure b is the microemulsion after the centrifugation test;

[0037] Figure 5 The pictures are of the microemulsion of Comparative Example 2 before and after the centrifugation test, wherein Figure a is the microemulsion before the centrifugation test, and Figure b is the microemulsion after the centrifugation test;

[0038] Figure 6 These are pictures of the microemulsion provided in Example 1 after high temperature testing at different temperatures;

[0039] Figure 7 This is a picture of the microemulsion provided in Example 1 after freeze-thaw testing;

[0040] Figure 8 The relationship between the concentration and pH value of the microemulsion in Example 5 after it is diluted with chicken manure water to different concentrations;

[0041] Fig. 9 The relationship between pH value and sterilization quantity after the microemulsion in Example 5 is diluted with chicken manure water to different concentrations;

[0042] Fig.10 The relationship between the concentration and the sterilization amount after the microemulsion in Example 5 is diluted to different concentrations with chicken manure water. DETAILED DESCRIPTION

[0043] The technical solution of the present invention is further described below by specific embodiments, which do not limit the protection scope of the present invention. Some non-essential modifications and adjustments made by others based on the concept of the present invention still fall within the protection scope of the present invention.

[0044] Experimental materials and sources

[0045] n-Butanol, Tween 80, 95% ethanol, and anhydrous ethanol were purchased from Xilong Scientific Co., Ltd.

[0046] Sodium butyrate, glyceryl tributyrate, and polyglycerol monolaurate were purchased from Longyan Xinao Biotechnology Co., Ltd.

[0047] Example 1

[0048] Example 1 provides a microemulsion that is odorless, antibacterial and bactericidal, and has a high content of butyrate, which includes, by mass percentage: 30% of a surfactant (Tween 80 and Span 80 mixed in a mass ratio of 1:1), 3% of a co-surfactant, 15% of tributyrin, 25% of sodium butyrate, and the rest is water.

[0049] Preparation method: Sodium butyrate powder is dissolved in water, and then the auxiliary surfactant n-butanol is added and stirred thoroughly. At the same time, the surfactant (Tween 80 and Span 80 are mixed in a mass ratio of 1:1) is mixed and dissolved in a stirring pot at a temperature of 40℃-60℃, and the oil phase glyceryl tributyrate is added to the mixed surfactant, and stirred thoroughly to mix into a uniform state. Finally, the aqueous solution containing n-butanol and sodium butyrate is gradually added dropwise to the mixed surfactant and oil phase, and stirred thoroughly for more than 30 minutes to obtain a clear, transparent, stable and water-soluble microemulsion (such as Figure 1 a).

[0050] Example 2

[0051] Example 2 provides a microemulsion that is odorless, antibacterial and bactericidal, and has a high content of butyrate, which includes, by mass percentage: 10% surfactant, 2% co-surfactant, 25% glyceryl tributyrate and polyglyceryl monolaurate (glyceryl tributyrate and polyglyceryl monolaurate are mixed in a mass ratio of 1:1), 30% sodium butyrate, and the rest is water.

[0052] Preparation method: Sodium butyrate powder is dissolved in water, and then 95% ethanol as a co-surfactant is added and stirred thoroughly. At the same time, the surfactant Tween 80 is dissolved in a stirring pot at a temperature of 40℃-60℃, and the oil phase glyceryl tributyrate and polyglycerol monolaurate (glyceryl tributyrate and polyglycerol monolaurate are mixed in a mass ratio of 1:1) are added to the dissolved surfactant, and stirred thoroughly to mix into a uniform state. Finally, the mixed aqueous solution containing 95% ethanol and sodium butyrate is gradually added dropwise to the mixed surfactant and oil phase, and stirred thoroughly for more than 30 minutes to obtain a clear, transparent, stable microemulsion with good water solubility (such as Figure 2 a).

[0053] Example 3

[0054] Example 3 provides a microemulsion that is odorless, antibacterial and bactericidal, and has a high butyrate content, which includes, by mass percentage: 11.8% of a surfactant, 2.12% of a co-surfactant, 22.22% of tributyrin and polyglycerol monolaurate (glycerol tributyrin and polyglycerol monolaurate are mixed in a mass ratio of 1:1), 32% of sodium butyrate, and the rest is water.

[0055] Preparation method: Preparation method: Sodium butyrate powder is dissolved in water, and then anhydrous ethanol, a co-surfactant, is added and stirred thoroughly. At the same time, the surfactant Tween 80 is dissolved in a stirring pot at a temperature of 40°C-60°C, and the oil phase glyceryl tributyrate and polyglycerol monolaurate (glyceryl tributyrate and polyglycerol monolaurate are mixed in a mass ratio of 1:1) are added to the dissolved surfactant, and stirred thoroughly to mix into a uniform state. Finally, the mixed aqueous solution containing anhydrous ethanol and sodium butyrate is gradually added dropwise to the mixed surfactant and oil phase, and stirred thoroughly for more than 30 minutes to obtain a clear, transparent, stable microemulsion with good water solubility (such as Figure 3 a).

[0056] Comparative Example 1

[0057] The difference between Comparative Example 1 and Example 1 is that sodium butyrate is not added to the aqueous solution of Comparative Example 1, and the rest of the preparation method is exactly the same as that of Example 1. The obtained microemulsion is as follows Figure 4 As shown in a.

[0058] Comparative Example 2

[0059] The difference between Comparative Example 2 and Example 2 is that no 95% ethanol is added to the aqueous solution of Comparative Example 2, and the rest of the preparation method is exactly the same as that of Example 2. The obtained microemulsion is as follows Figure 5 As shown in a.

[0060] Performance Testing

[0061] The microemulsions of Examples 1-3 and Comparative Examples 1-2 were subjected to the following stability tests:

[0062] 1) Centrifugation test: centrifuge at 3000 rpm for 30 min;

[0063] 2) High temperature test: The microemulsion was placed in an oven at 48°C-52°C for 2 months;

[0064] 3) Freeze-thaw test: The microemulsion was placed in a -18°C refrigerator and then thawed for 24 hours.

[0065] The test results are shown in Table 1:

[0066] Table 1 Microemulsion performance test results

[0067] Example Centrifuge test High temperature testing Freeze-thaw test Example 1 No stratification No stratification No stratification Example 2 No stratification No stratification No stratification Example 3 No stratification No stratification No stratification Comparative Example 1 No stratification Layering Layering Comparative Example 2 Layering Layering Layering

[0068] The centrifugal tests of Examples 1-3 and Comparative Examples 1-2 are as follows Figure 1-Figure 5 As shown, the high temperature test of the microemulsion provided in Example 1 is as follows Figure 6 As shown, the freeze-thaw test of the microemulsion provided in Example 1 is as follows Figure 7As shown; from the performance test results, it can be seen that the microemulsion prepared in the present application will not stratify during centrifugation and has good stability under high temperature and repeated freezing and thawing conditions.

[0069] Example 4

[0070] After the microemulsion provided in Example 1 was diluted with distilled water at a ratio of 1:100-1000, the pH of the aqueous solution decreased from 7.72 to 5.42. Detailed data are shown in Table 2.

[0071] Table 2 pH changes of microemulsion after dissolving in water

[0072] Dilution ratio pH Temperature / ℃ Undiluted 7.72 29.6 1:100 5.42 29.6 1:200 5.43 29.6 1:300 5.46 29.6 1:400 5.46 29.6 1:500 5.42 29.4 1:600 5.44 29.7 1:700 5.45 29.6 1:800 5.45 29.4 1:900 5.45 29.5 1:1000 5.42 29.0

[0073] The pH value of the microemulsion provided in Example 1 of the present invention drops to within the range of 5.42-5.46 after dilution. When the pH is within this range, the microemulsion provided by the present invention can play a broad-spectrum antibacterial role.

[0074] Example 5

[0075] The microemulsion provided in Example 1 was mixed with chicken manure water in a mass ratio of 3:1, 2:1, 1:1, 1:2, 1:3, 1:6, 1:24, 1:48, 1:96, 1:192, 1:384, 1:768, and 1:1536. After the microemulsion was diluted with chicken manure water to different concentrations, the relationship between its concentration and pH value is as follows: Figure 8 , and then use the ATP instrument to detect the colony situation to check the bactericidal and antibacterial effects. The ATP fluorescence detector is based on the principle of firefly luminescence and uses the "luciferase-luciferin system" to quickly detect adenosine triphosphate (ATP). Since all living cells contain a constant amount of ATP, the ATP content can clearly indicate the amount of microorganisms and other biological residues in the sample. The results are shown in Table 3. The relationship between different pH values ​​and the number of bactericidal Fig. 9 The relationship between different percentages of microemulsion and sterilization is as follows: Fig.10 .

[0076] Table 3 Bactericidal and antibacterial results of microemulsions at different concentrations

[0077]

[0078] in conclusion:

[0079] 1) When the microemulsion is diluted to 1:6 (i.e. 14.29%), the pH reaches the lowest value of 5.5. Subsequently, regardless of whether the concentration increases or decreases, the pH begins to increase, showing a check mark "√".

[0080] 2) When the microemulsion is diluted to 1:6 (i.e. 14.29%), the pH is 5.5, which is also the best concentration for bactericidal effect.

[0081] Example 6

[0082] Example 6 is the application of the microemulsion provided in Example 1 of the present invention in feed processing.

[0083] 1. Experimental Preparation

[0084] 1.1 Experimental origin: Guangdong Dada Industrial Co., Ltd.

[0085] 1.2 Experimental feed: lactating sow feed: self-prepared. Formula: 66.5% corn, 10% soybean cake, 8% wheat bran, 3% fish meal, 1.3% bone meal, 0.7% shell meal, 0.5% salt and 5% vitamin mineral additive premixed with feed, soybean oil and microemulsion of Example 1 (the addition ratio is as shown in the experimental design in the following table).

[0086] 2. Experimental design:

[0087]

[0088] 3. Experimental results:

[0089] 3.1 Feed quality observation:

[0090] hardness Powdering rate Moisture pH Bad smell Control group 27.4 3.9 12.8 5.95 obvious Experimental Group 1 36.5 4.8 12.3 5.89 Not obvious Experimental Group 2 28.7 5.8 12.9 5.99 Not obvious

[0091] The microemulsion provided by the present invention can replace part of soybean oil in lactating sow feed without obvious change in feed quality. At the same time, the microemulsion provided by the present invention can have a broad-spectrum antibacterial effect in feed application and reduce the obvious rancid taste caused by the use of sodium butyrate in feed.

Claims

1. A microemulsion composition, characterized in that: The microemulsion composition comprises polyglycerol fatty acid ester and fatty acid salt; The mass percentage of the polyglycerol fatty acid ester and the fatty acid salt is (5-35): (15-40).

2. The microemulsion composition according to claim 1, characterized in that: The polyglycerol fatty acid ester polyglycerol has a molecular number of 1-5, and the fatty acid includes butyric acid, caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid or linoleic acid; Preferably, the fatty acid comprises butyric acid or lauric acid; Preferably, the polyglycerol fatty acid ester is one or a combination of glycerol monobutyrate, glycerol dibutyrate, glycerol tributyrate, glycerol monolaurate, diglycerol laurate, and triglycerol laurate; Preferably, the fatty acid salt comprises butyrate; Preferably, the butyrate includes one or a combination of sodium butyrate, calcium butyrate, potassium butyrate and magnesium butyrate.

3. The microemulsion composition according to claim 1, characterized in that The microemulsion composition also includes a surfactant and a co-surfactant; Preferably, the surfactant is a Tween surfactant having an HLB value between 8 and 18 and / or a Span surfactant having an HLB value between 4.3 and 8.6; Preferably, the surfactant is one or a combination of Tween 20, Tween 40, Tween 60, Tween 80 and / or Span 20, Span 40, Span 60, Span 80; Preferably, the co-surfactant is at least one of anhydrous ethanol, 95% ethanol, and n-butanol.

4. The microemulsion composition according to any one of claims 1 to 3, characterized in that: The microemulsion composition contains the following substances in percentage by mass: Butyrate 5%-35%, polyglycerol fatty acid ester 15%-40%, surfactant 5%-35%, co-surfactant 1%-8%, and the balance is water; Preferably, the microemulsion composition contains the following substances in percentage by mass: Butyrate 10%-30%, polyglycerol fatty acid ester 20%-35%, surfactant 10%-30%, co-surfactant 3%-6%, and the balance is water.

5. The microemulsion composition according to any one of claims 1 to 4, characterized in that: The pH value of the microemulsion composition after being diluted 100-1000 times with water is 4-6.8; Preferably, the pH value of the microemulsion composition after being diluted 100-1000 times with water is 5-5.

8.

6. A method for preparing a microemulsion composition, characterized in that: The method comprises the following steps: Dissolve butyrate in water, adjust the pH value to 8-11, add a co-surfactant to obtain solution 1, mix the polyglycerol fatty acid ester with the surfactant to obtain solution 2, and mix solution 1 with solution 2 to obtain a microemulsion composition.

7. Use of the microemulsion composition according to any one of claims 1 to 5 or the microemulsion composition prepared by the method according to claim 6 in the preparation of antibacterial drugs, health products, food preservatives or feed; Preferably, the medicine, health product, food preservative or feed is a medicine, health product, food preservative or feed for preventing, treating or assisting in treating bacterial infection; Preferably, the bacteria are Gram-positive bacteria or Gram-negative bacteria; Preferably, the bacteria include Salmonella, Escherichia coli, Clostridium, Campylobacter, Enterococcus, Staphylococcus aureus.

8. A feed containing a microemulsion composition, characterized in that: The microemulsion composition is the microemulsion composition according to any one of claims 1 to 5 or the microemulsion composition prepared by the method according to claim 6; Preferably, the microemulsion composition is added to the feed in an amount of 2-40 kg per 2 tons of feed; Preferably, the microemulsion composition is added to the feed in an amount of 4-30 kg per 2 tons of feed; Preferably, the microemulsion composition is added to the feed in an amount of 4-20 kg per 2 tons of feed; Preferably, the amount of the microemulsion composition added to the feed is 4 kg per 2 tons of feed; Preferably, the microemulsion composition is added to the feed in an amount of 8 kg per 2 tons of feed.

9. The feed according to claim 8, characterized in that The feed also contains a premix and soybean oil; Preferably, the feed comprises corn, soybean cake, wheat bran, fish meal, bone meal, shell meal, salt and vitamin and mineral additives; Preferably, the feed comprises the following substances in percentage by weight: Corn 64.5%-68.5%, soybean cake 8%-12%, wheat bran 6%-10%, fish meal 1%-5%, bone meal 0.3%-2.3%, shell meal 0.5%-0.9%, salt 0.3%-0.7% and vitamin and mineral additives 3%-7%; Preferably, the feed comprises the following substances in percentage by weight: Corn 66.5%, soybean meal 10%, wheat bran 8%, fish meal 3%, bone meal 1.3%, shell meal 0.7%, salt 0.5% and vitamin and mineral additives 5%.

10. The feed according to any one of claims 8 to 9, characterized in that The feed can be used to inhibit the growth of microorganisms; Preferably, the microorganism is a bacterium; Preferably, the bacteria are Gram-positive bacteria or Gram-negative bacteria; preferably, the bacteria include Salmonella, Escherichia coli, Clostridium, Campylobacter, Enterococcus, Staphylococcus aureus.

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