Locally bacteriostatic spray film agent and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a locally bacteriostatic spray film agent and a preparation method thereof. The spray film agent comprises 2.0% w / v-10.0% w / v of natural product volatile oil, 0.1% w / v-1. 0% w / v of penetration enhancer and the balance of water; the penetration enhancer is peppermint oil or menthol. The natural products adopted in the invention can synergistically inhibit Candida albicans, can reduce mouth mucosa irritation to a certain extent, and can locally enrich drugs. The raw materials adopted by the invention are derived from natural products, are low in cost and wide in source, are finally embodied in the form of the spray film agent, are safe and reliable to use, do not have toxic or side effects, have a remarkable curative effect on local fungal infection, are short in treatment time, and have the effects of activating blood, relieving pain, promoting tissue regeneration, diminishing inflammation, detoxifying, converging and the like.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a local antibacterial spray film and a preparation method thereof. Background Art

[0002] Typical local fungal infection disease thrush is also known as acute pseudomembranous candidal stomatitis. It is a common manifestation of oral candida caused by pseudomembranous lesions of the oral mucosa caused by fungal infection of Candida species. It usually refers to the appearance of white dots or flakes on the tongue and oral mucosa in the shape of a goose mouth. This disease can be caused by congenital fetal heat or oral uncleanliness, which can lead to the exposure of filthy and toxic evil. Clinical treatment of thrush is more difficult. The general treatment is Western medicine infusion or topical oral mucosa. The treatment period is long, the effect is slow, and it is easy to rebound, which cannot achieve the purpose of treatment.

[0003] Conventional treatments for thrush mainly include topical and oral antifungals, which are available in different dosage forms. Let’s now discuss the types of these solutions, their dosage forms, and their limitations.

[0004] Existing drug delivery methods include:

[0005] 1) Topical antifungals: Common dosage forms include oral suspensions, lozenges, gels, and oral sprays. These drugs usually require multiple applications and need to be kept in the mouth for a period of time to ensure adequate contact with the infected area. There are some invariants for patients with limited mobility or who are unable to follow complex dosing instructions to complete treatment. In addition, some drugs may cause adverse reactions such as bitter taste, stinging, or swelling in the mouth.

[0006] 2) Systemic antifungal drugs: usually in the form of oral solids (such as tablets, capsules) or liquids (such as oral suspensions), and may require intravenous injection in severe cases. These drugs may cause more serious side effects, such as liver toxicity, gastrointestinal discomfort, or rash. They may also interact with other drugs.

[0007] Since current injections are inconvenient to administer and carry, and have a high incidence of adverse reactions, research has been conducted on improving the stability and reducing the risk of hemolysis in existing injections. The results suggest that in the future, it is possible to develop dosage forms that can improve compliance, safety, effectiveness, and ease of administration by changing the formula of the injection or using new dosage forms. Summary of the invention

[0008] In order to solve the problems existing in the prior art, the present invention provides a local antibacterial spray film with good stability, convenient administration, long action time and the ability to meet the local antibacterial medication needs for thrush and the like, and a preparation method thereof.

[0009] To achieve the above purpose, the present invention adopts the following technical solution:

[0010] A natural product spray film for local antibacterial, the spray film comprises 2.0% w / v to 10.0% w / v of natural product volatile oil and 0.1% w / v to 1.0% w / v of a penetration enhancer, the balance being water; the penetration enhancer is peppermint oil or menthol.

[0011] The natural product volatile oil is one or more of wormwood volatile oil, ginseng essential oil, oxyphylla seed oil, turmeric oil, zedoary turmeric volatile oil, patchouli oil, dandelion oil and peppermint oil.

[0012] The topical antibacterial spray film comprises, by mass percentage, 2.0% w / v to 10.0% w / v of natural product volatile oil, 0.1% w / v to 1.0% w / v of peppermint oil, 0.5% w / v to 1.0% w / v of thickener, 2.0% w / v to 10.0% w / v of solubilizer, 1.0% w / v to 5.0% w / v of emulsifier, 2.0% w / v to 5.0% w / v of calcium salt compounds, 0.05% w / v to 1.4% w / v of other auxiliary agents, and 66.6% w / v to 92.35% w / v of deionized water.

[0013] The other auxiliary agents are one or more of an auxiliary emulsifier, a flavoring agent and an osmotic pressure regulator.

[0014] The topical antibacterial spray film comprises, by mass percentage, 2.0% w / v to 10.0% w / v of natural product volatile oil, 0.1% w / v to 1.0% w / v of peppermint oil, 0.5% w / v to 1.0% w / v of thickener (preferably 0.5% w / v to 0.8% w / v), 2.0% w / v to 10.0% w / v of solubilizer (preferably 8.0% w / v to 10.0% w / v), and 1.0% w / v to 5. 0% w / v (preferably 2.0% w / v to 3.0% w / v), calcium salt compound 2.0% w / v to 5.0% w / v, co-emulsifier 0.05% w / v to 0.2% w / v, flavoring agent 0.05% w / v to 0.1% w / v and osmotic pressure regulator 0.1% w / v to 1.0% w / v, deionized water 66.7% w / v to 92.2% w / v (preferably 68.9% w / v to 85.2% w / v).

[0015] The thickener may be one or more of cellulose ether compounds, chitosan, sodium alginate, xanthan gum, carbomer or sodium carboxymethyl cellulose;

[0016] The cellulose ether compound may be one selected from methyl cellulose, hydroxyethyl methyl cellulose, hydroxypropyl methyl cellulose, hydroxybutyl methyl cellulose, ethyl methyl cellulose, carboxymethyl methyl cellulose, ethyl cellulose, ethyl hydroxyethyl cellulose, hydroxyethyl cellulose and hydroxypropyl cellulose, or a combination thereof.

[0017] The solubilizer may be one or more of alcohol, ester or ether; preferably, the solubilizer may be one or more of propylene glycol, glycerol, polysorbate 80, polysorbate 20 or poloxamer 188;

[0018] The emulsifier may be one or more of alcohol, ester or ether; preferably, the emulsifier may be one or more of sorbitan monooleate, sorbitan trioleate, glyceryl mono- and distearate, polyglyceryl ricinoleate, and lecithin.

[0019] The calcium salt compound may be one or more of calcium gluconate, calcium hydrogen phosphate, calcium lactate and calcium chloride;

[0020] The auxiliary emulsifier is one or more of sucrose fatty acid ester, sucrose laurate, sucrose palmitate, sucrose oleate, and sorbitan trioleate; preferably, the auxiliary emulsifier is sucrose fatty acid ester;

[0021] The flavoring agent is one or more of sucrose, chitosan, steviol glycoside, sucralose, and edible flavors; the flavoring agent is sucralose and / or edible flavors;

[0022] The osmotic pressure regulator is one or more of trisodium citrate, disodium hydrogen phosphate, sodium dihydrogen phosphate, glucose, and sodium chloride. The osmotic pressure regulator is sodium chloride.

[0023] A method for preparing the local antibacterial spray film, characterized in that:

[0024] (1) adding a thickener into deionized water, stirring to dissolve, adding a flavoring agent, a solubilizing agent, and an osmotic pressure regulator, and stirring to obtain a mixture A;

[0025] (2) adding the co-emulsifier to the penetration enhancer, heating and dissolving, adding the co-emulsifier to the natural product and the emulsifier, stirring evenly, and adding the calcium salt aqueous solution thereto to prepare a mixture B;

[0026] (3) Mixture A and mixture B are combined and stirred to obtain the spray film.

[0027] The spray film is a suspension containing water, and the average particle size of the fine particles sprayed out is 0.5 μm to 5 μm; preferably, the average particle size is 0.5 μm to 0.9 μm.

[0028] Beneficial effects of the present invention:

[0029] The present invention mainly acts on the oral cavity, and the formula has a strong inhibitory effect on Candida albicans, and the mouthfeel is better, and the oral coolness has a sweet taste, the oral coolness lasts for a long time, the sense organ is good, and there is no irritation to the oral mucosa. It is suitable for sick children and the elderly to use, and the toxic and side effects are reduced while enhancing the therapeutic effect. After the preparation is sprayed out by the packaging material, a film is spontaneously formed to coat the affected part, increasing the residence time and concentration of the drug in the affected part. The volatile oil of Curcuma zedoariae and the peppermint oil synergistically inhibit Candida albicans, and the irritation of the oral mucosa can be reduced to a certain extent. The present invention belongs to a natural product spray film, and the raw material cost adopted in the present invention is low, the source is wide, and it is finally embodied in the form of a spray film, the preparation operation is simple, the use is safe and reliable, there is no toxic and side effect, the curative effect of treating thrush is remarkable, and the treatment time is short, and there are the effects of promoting blood circulation, relieving pain, promoting myogenic inflammation, detoxification, astringency, etc. The present invention has a good therapeutic effect on thrush, and has the characteristics of high efficacy, no recurrence, and convenient to carry. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The HPLC chromatogram of the main components of the zedoary turmeric oil provided in the embodiment of the present invention.

[0031] Figure 2 This is an appearance diagram of the liquid contents of the spray film provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0032] The specific implementation modes of the present invention are further described below in conjunction with examples. It should be noted that the specific implementation modes described here are only for illustrating and explaining the present invention, and are not limited to the present invention.

[0033] Extraction of volatile oil from Rhizoma Curcumae in the following examples

[0034] The invention adopts ultrasonic pretreatment-microwave method to extract zedoary turmeric oil. Take dried zedoary turmeric slices, crush them, pass through a 50-mesh sieve to obtain zedoary turmeric powder, add them into excess ethanol, and perform ultrasonic treatment for 30 minutes; remove 70% of organic solvent by vacuum distillation; add excess water, and perform 200W microwave extraction; after the extraction is complete, collect the oil phase, add a conventional desiccant to dry, and remove the remaining organic solvent by vacuum distillation to obtain zedoary turmeric volatile oil.

[0035] The powder of medicinal materials was treated with ultrasonic wave by organic solvent, and physical crushing was achieved during the ultrasonic treatment process, so that most of the volatile oil was dissolved in the solvent, and then the organic solvent was removed to obtain the volatile oil. The yield was measured to be 3.5% to 4.7%. The volatile oil extracted by HPLC method contained 6 effective components, including curcuma dione, curcuma alcohol, germacrone, curcumene, furadiene, and β-elemene. The results are as follows Figure 1As shown, this method has the characteristics of low cost and high efficiency.

[0036] Meanwhile, the volatile oil of Curcuma zedoaria used in the following examples can also be obtained through commercial means.

[0037] Example 1

[0038] Experiment on the synergistic antibacterial effect of volatile oil of Curcuma zedoaria and peppermint oil

[0039] The Oxford cup method was used to determine the optimal ratio of Curcuma volatile oil and peppermint oil: Curcuma volatile oil and peppermint oil were selected as the mixed oil, and the same volume of culture medium containing Candida albicans and the mixed oil obtained above were added into the Oxford cup. After culturing for 48 hours in a conventional manner, the size of the inhibition zone was observed; at the same time, nystatin was used as a control.

[0040] The culture medium containing Candida albicans is prepared by culturing Candida albicans in a beef extract peptone culture medium until the logarithmic growth phase, and then the culture medium is used for later use.

[0041] The experimental results are shown in Table 1

[0042] Table 1 Results of antibacterial activity determination of mixed oil phases in different proportions

[0043]

[0044] It can be seen from Table 1 that the two drugs have a strong antibacterial effect against Candida albicans, showing that the penetration enhancer is conducive to the main drug to exert a better antibacterial effect.

[0045] Example 2

[0046] Local antibacterial spray film, calculated by mass percentage, includes 2.5% zedoary volatile oil, 0.25%

[0047] Peppermint oil, 0.75% sodium alginate, 8.5% Tween 80, 2% sorbitan monooleate, 4.0% calcium chloride, 0.2% sucrose fatty acid ester, 0.1% edible flavor, 0.1% sucralose, 1% sodium chloride, and the balance is deionized water.

[0048] The preparation method is as follows: according to the above percentage, sodium alginate is added into deionized water, stirred and dissolved, polysorbate 80 and sodium chloride are added, and then sucralose and edible flavor are added and stirred evenly to obtain a mixture A; sucrose fatty acid ester is added into peppermint oil and heated to dissolve, and then added into zedoary turmeric volatile oil and sorbitan monooleate, and under high-speed dispersion conditions, a calcium chloride aqueous solution is added thereto to obtain a mixture B;

[0049] The mixture A and the mixture B are combined and stirred evenly to obtain the spray film.

[0050] Example 3

[0051] Local antibacterial spray film, calculated by mass percentage, includes 2.5% zedoary volatile oil, 0.20%

[0052] Peppermint oil, 0.7% sodium alginate, 8.5% Tween 80, 2.0% sorbitan monooleate, 4.0% calcium chloride, 0.2% sucrose fatty acid ester, 0.1% edible flavor, 0.1% sucralose, 1% sodium chloride, and the balance is deionized water.

[0053] The preparation method comprises the following steps: adding sodium alginate into deionized water according to the above percentage, stirring and dissolving, adding polysorbate 80 and sodium chloride, and then adding sucralose and edible flavoring and stirring evenly to obtain a mixture A; adding sucrose fatty acid ester into peppermint oil, heating and dissolving, adding into zedoary turmeric volatile oil and sorbitan monooleate, and adding calcium chloride aqueous solution thereto under high-speed dispersion conditions to obtain a mixture B; combining the mixture A and the mixture B, stirring evenly, and obtaining the spray film.

[0054] Example 4

[0055] The topical antibacterial spray film comprises, by weight percentage, 2.0% zedoary volatile oil, 0.25% peppermint oil, 0.75% sodium alginate, 8.0% Tween 80, 2.0% sorbitan monooleate, 4.0% calcium chloride, 0.2% sucrose fatty acid ester, 0.1% edible flavor, 0.1% sucralose, 0.5% sodium chloride, and the balance is deionized water.

[0056] The preparation method comprises the following steps: adding sodium alginate into deionized water according to the above percentage, stirring and dissolving, adding polysorbate 80 and sodium chloride, and then adding sucralose and edible flavoring and stirring evenly to obtain a mixture A; adding sucrose fatty acid ester into peppermint oil, heating and dissolving, adding into zedoary turmeric volatile oil and sorbitan monooleate, and adding calcium chloride aqueous solution thereto under high-speed dispersion conditions to obtain a mixture B; combining the mixture A and the mixture B, stirring evenly, and obtaining the spray film.

[0057] Example 5

[0058] The topical antibacterial spray film comprises, by weight percentage, 2.5% zedoary volatile oil, 0.25% peppermint oil, 0.75% sodium alginate, 8.5% Tween 80, 1.5% sorbitan monooleate, 4.0% calcium chloride, 0.2% sucrose fatty acid ester, 0.1% edible flavor, 0.1% sucralose, 1% sodium chloride, and the balance is deionized water.

[0059] The preparation method comprises the following steps: adding sodium alginate into deionized water according to the above percentage, stirring and dissolving, adding polysorbate 80 and sodium chloride, and then adding sucralose and edible flavoring and stirring evenly to obtain a mixture A; adding sucrose fatty acid ester into peppermint oil, heating and dissolving, adding into zedoary turmeric volatile oil and sorbitan monooleate, and adding calcium chloride aqueous solution thereto under high-speed dispersion conditions to obtain a mixture B; combining the mixture A and the mixture B, stirring evenly, and obtaining the spray film.

[0060] Example 6

[0061] The topical antibacterial spray film comprises, by weight percentage, 2.5% zedoary volatile oil, 0.25% peppermint oil, 0.75% sodium alginate, 8.5% Tween 80, 2.0% sorbitan monooleate, 3.5% calcium chloride, 0.2% sucrose fatty acid ester, 0.1% edible flavor, 0.12% sucralose, 1% sodium chloride, and the balance is deionized water.

[0062] The preparation method comprises the following steps: adding sodium alginate into deionized water according to the above percentage, stirring and dissolving, adding polysorbate 80 and sodium chloride, and then adding sucralose and edible flavoring and stirring evenly to obtain a mixture A; adding sucrose fatty acid ester into peppermint oil, heating and dissolving, adding into zedoary turmeric volatile oil and sorbitan monooleate, and adding calcium chloride aqueous solution thereto under high-speed dispersion conditions to obtain a mixture B; combining the mixture A and the mixture B, stirring evenly, and obtaining the spray film.

[0063] Example 7

[0064] The topical antibacterial spray film comprises, by weight percentage, 3.0% zedoary volatile oil, 0.15% peppermint oil, 0.75% sodium alginate, 8.5% Tween 80, 2.0% sorbitan monooleate, 4.0% calcium chloride, 0.2% sucrose fatty acid ester, 0.1% edible flavor, 0.1% sucralose, 0.8% sodium chloride, and the balance is deionized water.

[0065] The preparation method comprises the following steps: adding sodium alginate into deionized water according to the above percentage, stirring and dissolving, adding polysorbate 80 and sodium chloride, and then adding sucralose and edible flavoring and stirring evenly to obtain a mixture A; adding sucrose fatty acid ester into peppermint oil, heating and dissolving, adding into zedoary turmeric volatile oil and sorbitan monooleate, and adding calcium chloride aqueous solution thereto under high-speed dispersion conditions to obtain a mixture B; combining the mixture A and the mixture B, stirring evenly, and obtaining the spray film.

[0066] Example 8

[0067] The topical antibacterial spray film comprises, by weight percentage, 3.0% zedoary volatile oil, 0.15% peppermint oil, 0.75% sodium alginate, 10.0% Tween 80, 2.0% sorbitan monooleate, 4.0% calcium chloride, 0.1% sucrose fatty acid ester, 0.1% edible flavor, 0.1% sucralose, 1.0% sodium chloride, and the balance is deionized water.

[0068] The preparation method comprises the following steps: adding sodium alginate into deionized water according to the above percentage, stirring and dissolving, adding polysorbate 80 and sodium chloride, and then adding sucralose and edible flavoring and stirring evenly to obtain a mixture A; adding sucrose fatty acid ester into peppermint oil, heating and dissolving, adding into zedoary turmeric volatile oil and sorbitan monooleate, and adding calcium chloride aqueous solution thereto under high-speed dispersion conditions to obtain a mixture B; combining the mixture A and the mixture B, stirring evenly, and obtaining the spray film.

[0069] Example 9

[0070] The topical antibacterial spray film comprises, by weight percentage, 2.0% zedoary volatile oil, 0.25% peppermint oil, 0.75% sodium alginate, 8.5% Tween 80, 2% sorbitan monooleate, 4.0% calcium chloride, 0.2% sucrose fatty acid ester, 0.1% edible flavor, 0.1% sucralose, 0.8% sodium chloride, and the balance is deionized water.

[0071] The preparation method comprises the following steps: adding sodium alginate into deionized water according to the above percentage, stirring and dissolving, adding polysorbate 80 and sodium chloride, and then adding sucralose and edible flavoring and stirring evenly to obtain a mixture A; adding sucrose fatty acid ester into peppermint oil, heating and dissolving, adding into zedoary turmeric volatile oil and sorbitan monooleate, and adding calcium chloride aqueous solution thereto under high-speed dispersion conditions to obtain a mixture B; combining the mixture A and the mixture B, stirring evenly, and obtaining the spray film.

[0072] Example 10

[0073] The topical antibacterial spray film comprises, by weight percentage, 3.0% zedoary volatile oil, 0.15% peppermint oil, 1.0% sodium alginate, 8.5% Tween 80, 3.5% sorbitan monooleate, 5% calcium chloride, 0.05% sucrose fatty acid ester, 0.1% edible flavor, 0.1% sucralose, 0.8% sodium chloride, and the balance is deionized water.

[0074] The preparation method comprises the following steps: adding sodium alginate into deionized water according to the above percentage, stirring and dissolving, adding polysorbate 80 and sodium chloride, and then adding sucralose and edible flavoring and stirring evenly to obtain a mixture A; adding sucrose fatty acid ester into peppermint oil, heating and dissolving, adding into zedoary turmeric volatile oil and sorbitan monooleate, and adding calcium chloride aqueous solution thereto under high-speed dispersion conditions to obtain a mixture B; combining the mixture A and the mixture B, stirring evenly, and obtaining the spray film.

[0075] Embodiment 11

[0076] The topical antibacterial spray film comprises, by weight percentage, 2.5% zedoary volatile oil, 0.28% peppermint oil, 0.75% sodium alginate, 8.5% Tween 80, 1.5% sorbitan monooleate, 4% calcium chloride, 0.2% sucrose fatty acid ester, 0.1% edible flavor, 0.1% sucralose, 1.0% sodium chloride, and the balance is deionized water.

[0077] The preparation method comprises the following steps: adding sodium alginate into deionized water according to the above percentage, stirring and dissolving, adding polysorbate 80 and sodium chloride, and then adding sucralose and edible flavoring and stirring evenly to obtain a mixture A; adding sucrose fatty acid ester into peppermint oil, heating and dissolving, adding into zedoary turmeric volatile oil and sorbitan monooleate, and adding calcium chloride aqueous solution thereto under high-speed dispersion conditions to obtain a mixture B; combining the mixture A and the mixture B, stirring evenly, and obtaining the spray film.

[0078] Example 2-11 Formulation Characterization Evaluation

[0079] Take a certain amount of topical antibacterial spray film in a 250mL beaker, and use a rotational viscometer to measure the viscosity of the preparations of Examples 2 to 11; Particle size determination: add deionized water to the wet sampler to the upper edge of the base, adjust the speed to 3000r / min, perform system centering, select liposomes (refractive index 1.47, absorbance 0.001) as the material, deionized water as the dispersion medium, select general mode, single sampling time and background acquisition time are both 5s, sampling times 3 times, time interval 0s, analysis range in advanced parameters is set to 0.02-2000μm, result conversion selects volume, particle type selects irregular, analysis sensitivity selects general (default), and set the shading ratio to 5%-20% in the prompt. Sample determination: click start, subtract blank background, add sample drop by drop, when the shading ratio is greater than 5% and remains relatively stable, click sample measurement, if the data fluctuates greatly, click sample measurement again for repeated measurement.

[0080] The results are shown in Table 2

[0081] Table 2 Particle size and viscosity test results of local antibacterial spray film

[0082] sample Particle size (μm) Viscosity (m·Pas) Example 2 0.9125 9.59±0.17 Example 3 0.8754 8.38±0.45 Example 4 0.9631 9.21±0.42 Example 5 1.052 10.54±0.35 Example 6 2.027 15.79±0.17 Example 7 0.7586 10.56±0.45 Example 8 2.527 15.72±0.12 Example 9 3.920 15.88±0.12 Example 10 4.437 15.33±0.51 Embodiment 11 0.8765 8.79±0.71

[0083] As can be seen from Table 2, the average particle size of Examples 2 to 11 is about 1 μm to 5 μm, and the viscosity range is between 8.0 and 16.0, and Example 7 has the smallest particle size and appropriate viscosity. Figure 2 Under an optical microscope, an obvious double-layer structure can be seen, which is conducive to ejection.

Claims

1. A natural product spray film for local antibacterial, characterized in that: The spray film comprises 2.0% w / v to 10.0% w / v of natural product volatile oil and 0.1% w / v to 1.0% w / v of penetration enhancer, and the balance is water; the penetration enhancer is peppermint oil or menthol.

2. The local antibacterial spray film according to claim 1, characterized in that: The natural product is one or more of volatile oil of Artemisia argyi, ginseng essential oil, Alpinia oxyphylla seed oil, turmeric oil, volatile oil of Curcuma zedoaria, patchouli oil, dandelion oil and peppermint oil.

3. The local antibacterial spray film according to claim 1, characterized in that: The local antibacterial spray film comprises, by mass percentage, 2.0% w / v to 10.0% w / v of natural product volatile oil, 0.1% w / v to 1.0% w / v of peppermint oil, 0.5% w / v to 1.0% w / v of thickener, 2.0% w / v to 10.0% w / v of solubilizer, 1.0% w / v to 5.0% w / v of emulsifier, 2.0% w / v to 5.0% w / v of calcium salt compounds, 0.05% w / v to 1.4% w / v of other auxiliary agents, and 66.6% to 92.35% of deionized water.

4. The local antibacterial spray film according to claim 3, characterized in that: The other auxiliary agents are one or more of an auxiliary emulsifier, a flavoring agent and an osmotic pressure regulator.

5. The local antibacterial spray film according to claim 3 or 4, characterized in that: The topical antibacterial spray film comprises, by mass percentage, 2.0% w / v to 10.0% w / v of natural product volatile oil, 0.1% w / v to 1.0% w / v of peppermint oil, 0.5% w / v to 1.0% w / v of thickener, 2.0% w / v to 10.0% w / v of solubilizer, 1.0% w / v to 5.0% w / v of emulsifier, 2.0% w / v to 5.0% w / v of calcium salt compound, 0.05% w / v to 0.2% w / v of auxiliary emulsifier, 0.05% w / v to 0.1% w / v of flavoring agent, 0.1% w / v to 1.0% w / v of osmotic pressure regulator, and 66.7% to 92.2% of deionized water.

6. The local antibacterial spray film according to claim 5, characterized in that: The thickener may be one or more of cellulose ether compounds, chitosan, sodium alginate, xanthan gum, carbomer or sodium carboxymethyl cellulose; The solubilizing agent may be one or more of alcohol, ester or ether; The emulsifier may be one or more of alcohol, ester or ether; The calcium salt compound may be one or more of calcium gluconate, calcium hydrogen phosphate, calcium lactate and calcium chloride; The auxiliary emulsifier is sucrose fatty acid ester; The flavoring agent is sucralose and / or edible flavoring; The osmotic pressure regulator is sodium chloride.

7. The local antibacterial spray film according to claim 6, characterized in that: The solubilizer may be one or more of propylene glycol, glycerol, polysorbate 80, polysorbate 20 or poloxamer 188; The emulsifier can be one or more of sorbitan monooleate, sorbitan trioleate, glyceryl mono- and distearate, polyglyceryl ricinoleate, and lecithin.

8. A method for preparing the local antibacterial spray film according to claim 1, characterized in that: (1) adding a thickener into deionized water, stirring to dissolve, adding a flavoring agent, a solubilizing agent, and an osmotic pressure regulator, and stirring to obtain a mixture A; (2) adding the co-emulsifier to the penetration enhancer, heating and dissolving, adding the co-emulsifier to the natural product and the emulsifier, stirring evenly, and adding the calcium salt aqueous solution thereto to prepare a mixture B; (3) Mixture A and mixture B are combined and stirred to obtain the spray film.

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