Self-driven microarray micro-fluidic chip with space coding function
By designing a self-driven microarray microfluidic chip with spatial coding function, using molecular printing and lamination technology, multiple parallel detection and analysis of the air pressure self-driven microfluidic chip are realized, which solves the problem of insufficient single sample detection capability in the existing technology, improves the portability and operation simplicity of the chip, and is suitable for POCT detection.
Patent Information
- Application Number
- CN202510082024.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-20
- Publication Date
- 2025-04-15
AI Technical Summary
The existing air pressure self-driven microfluidic chips lack the ability to perform multiple detection and analysis of single samples, which limits their application in POCT.
A self-driven microarray microfluidic chip with spatial coding function was designed, including molecularly printed PDMS sheets, sample mixed PDMS sheets and self-energized sample-loaded PDMS sheets. The finished chip is formed by lamination, and multiple sets of parallel runners and gas chambers are designed using air pressure drive, and multiple parallel detection and analysis are realized.
It realizes the multiple parallel detection and analysis capabilities of the air pressure self-driven microfluidic chip, improves the accuracy of diagnosis, and makes the chip more portable and easy to operate, reduces the risk of cross-contamination, and adapts to the detection needs of POCT.
Smart Images
Figure CN119823863A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to a test instrument, in particular to the preparation of a gas pressure self-driven microarray microfluidic chip with a spatial encoding function. Background Art
[0002] Microfluidic chips have the characteristics of high throughput, portability, and low reagent consumption, and can be integrated into independent "chip laboratory" systems. They have several advantages over traditional macroscopic systems, including reduced sample and reagent volume, faster analysis time, and the ability to integrate multiple functions into a single chip. In order to enable microfluidic chips to have more powerful sample extraction, separation, and multiplexed array detection functions, the chip usually needs to be connected to an external pump or power supply, but this limits the use of the chip in resource-poor areas. At the same time, complex operations are not conducive to the commercialization of the chip and do not meet the needs of POCT. Pressure-driven microfluidic chips can work in resource-limited environments due to their self-driving characteristics, and have the advantages of portability, easy operation, and reduced cross-contamination. In medicine, multiple biochemical indicators are usually used to determine diseases. In POCT detection, multi-indicator parallel detection is also very important. However, the current pressure-driven microfluidic chips lack the ability to perform multiple detection and analysis on a single sample, which limits their further application in POCT.
[0003] In the present invention, we have developed a self-driven microarray microfluidic chip with spatial encoding function. Molecular printing PDMS sheets are used for functional modification of glass slide substrates, and multiple sets of parallel flow channels can modify a variety of different recognition molecules, such as nucleic acids, antibodies, etc. After the functional modification of the glass slide is completed, the sample-carrying PDMS sheet and the sample-mixing PDMS sheet are precisely stacked, so that the sample-carrying PMDS sheet has the sample-inlet hole aligned with the sample-outlet hole of the sample-mixing PDMS sheet, and are placed in a vacuum sealed bag to form a finished chip for standby use. Summary of the invention
[0004] The technical problem to be solved by the present invention is to endow an air pressure self-driven microfluidic chip with the ability to perform multiple parallel detection and analysis on samples.
[0005] A self-driving microarray microfluidic chip with spatial encoding function, characterized in that: A self-driven microarray microfluidic chip with spatial encoding function, characterized in that it comprises a molecular printing PDMS sheet that can be stacked on a glass slide for use, a sample mixing PDMS sheet and a self-powered sample loading PDMS sheet; the molecular printing PDMS sheet has 11 groups of flow channels arranged in parallel, which are used for functionalization of the glass slide after punching; the sample mixing PDMS sheet has 3 sample addition holes and 1 outlet, which is placed on the sample loading PDMS sheet for use; the self-powered sample loading PDMS sheet has an S-shaped flow channel, a plurality of microarrays and a plurality of air chambers placed in parallel that are perpendicular to and communicate with the flow channel, and are placed in a vacuum sealed bag after stacking to form a finished chip.
[0006] (1) The air pressure self-driven microfluidic chip is endowed with the ability to perform parallel detection and analysis of samples, ensuring the accuracy of diagnosis.
[0007] (2) Make the air pressure self-driven microfluidic chip more portable and easier to operate, with lower cross contamination, and more suitable for POCT detection needs. BRIEF DESCRIPTION OF THE DRAWINGS
[0008] Figure 1 A schematic diagram of the device described in this article. DETAILED DESCRIPTION
[0009] In order to better understand the present invention, the content of the present invention is further explained below in conjunction with embodiments, but the content of the present invention is not limited to the following implementation. Example
[0010] A pneumatic self-driven microarray microfluidic chip with spatial encoding function, such as Figure 1 As shown, it includes a molecular printing PDMS sheet that can be stacked on a glass slide, a sample mixing PDMS sheet and a self-powered sample loading PDMS sheet; the molecular printing PDMS sheet has 11 sets of flow channels arranged in parallel, which are used for functionalization of the glass slide after punching; the sample mixing PDMS sheet has 3 sample addition holes and 1 outlet, which is placed on the sample loading PDMS sheet for use; the self-powered sample loading PDMS sheet has an S-shaped flow channel, a plurality of microarrays perpendicular to and communicating with the flow channel, and a plurality of air chambers placed in parallel, which are placed in a vacuum sealed bag after stacking to form a finished chip.
[0011] When the above-mentioned microfluidic chip is used, the vacuum sealing bag is opened, the chip is taken out, and the reaction solutions of 1 to 3 samples are quickly added to the sample loading holes in sequence. Since PDMS has a certain air permeability, the reaction solution will quickly fill the S-shaped flow channel of the self-powered sample-carrying PDMS sheet under the action of the parallel air chamber, and fill up multiple groups of microarrays that are orthogonal to the printing flow channel. At this time, the biological molecules on the printing flow channel will specifically bind to the molecules in the sample reaction solution, thereby leaving a quantitative signal on the chip. Example
[0012] A pneumatic self-driven microarray microfluidic chip with spatial encoding function, such as Figure 1 As shown, it includes a molecular printing PDMS sheet that can be stacked on a glass slide, a sample mixing PDMS sheet and a self-powered sample loading PDMS sheet; the molecular printing PDMS sheet has 11 sets of flow channels arranged in parallel, which are used for functionalization of the glass slide after punching; the sample mixing PDMS sheet has 3 sample addition holes and 1 outlet, which is placed on the sample loading PDMS sheet for use; the self-powered sample loading PDMS sheet has an S-shaped flow channel, a plurality of microarrays perpendicular to and communicating with the flow channel, and a plurality of air chambers placed in parallel, which are placed in a vacuum sealed bag after stacking to form a finished chip.
[0013] The above-mentioned microfluidic chip can be used for quantitative detection of miRNA. The molecular printing PDMS sheet is used to print a variety of nucleic acid capture probes on the aldehyded glass slide. After printing, the microfluidic chip is sealed with BSA solution. The sample to be tested is first added to the pre-reaction solution containing DNAzyme and Taqman probe and reacted at 30 °C for 30 min. After the reaction, the reaction solution is added to the sample well and left horizontally to wait for the solution to fill the entire flow channel. The cleaved encoded Taqman probe will be captured by the capture probe, leaving a fluorescent signal on the chip. At this time, a handheld fluorescent device can be used or a microarray fluorescent scanner can be placed after sufficient cleaning for qualitative and quantitative detection.
Claims
1. A self-driving microarray microfluidic chip with spatial encoding function, characterized in that: It comprises a molecular printing PDMS sheet which can be stacked on a glass slide for use, a sample mixing PDMS sheet and a self-powered sample loading PDMS sheet; the molecular printing PDMS sheet has 11 sets of flow channels arranged in parallel, which are used for functionalizing the glass slide after being punched; the sample mixing PDMS sheet has 3 sample addition holes and 1 outlet, which is placed on the sample loading PDMS sheet for use; the self-powered sample loading PDMS sheet has an S-shaped flow channel, a plurality of microarrays which are perpendicular to and communicate with the flow channel, and a plurality of air chambers which are placed in parallel, which are placed in a vacuum sealing bag after being stacked to form a finished chip.
2. The spatially encoded array microfluidic chip according to claim 1, characterized in that: A glass slide on which a plurality of groups of biomolecules are printed using a molecular printing PDMS sheet is used as a chip substrate, and the microarray and the molecular printing flow channel are orthogonal on the glass slide plane.
3. The spatially encoded array microfluidic chip according to claim 1, characterized in that: A finished chip is formed by stacking a PDMS sheet with a self-driven sample loading function and a PDMS sheet with a sample mixing function on a base glass slide.