Liquid chromatographic column capable of replacing filler and use method of liquid chromatographic column

Abstract

The liquid chromatographic column comprises a column pipe, the column pipe is internally provided with the filler, pipe heads are fixed to the two ends of the column pipe correspondingly, the inner diameter of each pipe head is larger than that of the column pipe, the surface of each pipe head is fixedly sleeved with a detachable column head cover, the outer side wall of each pipe head is provided with a clamping assembly used for fixing the corresponding column head cover, and the clamping assemblies are fixedly connected with the column head covers. And a sieve plate and a blade ring are clamped in the pipe head, the sieve plate is located on the side close to the column pipe, and rubber protection plugs are clamped in the two column head covers. When the packing is replaced, the clamping rod is firstly extruded, then the column head covers are detached from the surface of the tube head, the sieve plate and the blade ring are taken out, after the column head covers, the blade ring and the sieve plate at the two ends are all detached, the original packing in the column tube is taken out, new packing is added and compacted, and finally the column head covers, the blade ring and the sieve plate are installed. The two column head covers can be rapidly fixed to the surfaces of the two pipe heads through the clamping assemblies, and the sieve plates and the blade rings in the pipe heads can be taken out after the column head covers are detached, so that filler in the column pipes can be conveniently and rapidly replaced.

Description

Technical Field

[0001] The invention relates to the technical field of chromatographic columns, in particular to a liquid chromatographic column with replaceable fillers and a use method thereof. Background Art

[0002] Liquid chromatography is a separation technology widely used in biology, chemistry, environment and other fields. Its principle is to use column packing to separate the mixture and achieve separation through the difference in the distribution coefficient (K value) of different components in the column packing. As the core component of the liquid chromatography system, the liquid chromatography column can directly affect the sample separation effect and separation rate, and is one of the key components of liquid chromatography separation. The liquid chromatography column consists of two parts: the column shell and the column packing. The column shell includes the column body and the column cover, and the column packing is filled inside.

[0003] With the use of liquid chromatography column, the performance of filler will gradually decline with the increase of time and use, resulting in poor separation effect. In addition, impurities in the mobile phase and sample will contaminate the filler, further affecting the column efficiency and separation effect. In addition, the column caps at both ends of the existing chromatographic column are connected to the column body by threads, and frequent disassembly and replacement are prone to slipping, and the threaded part is also prone to rust and difficult to disassemble and assemble. In addition, after removing the column cap, the sieve plate is located inside the two ends of the column body. Since the column body is relatively thin, it is difficult to take out the sieve plate. Therefore, in view of the above-mentioned shortcomings, the present invention proposes a liquid chromatography column with replaceable filler and a method for using the same. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings in the prior art and to provide a liquid chromatography column with replaceable fillers and a method for using the same.

[0005] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a liquid chromatography column with replaceable filler, comprising a column tube, wherein the filler is arranged inside the column tube, and tube heads are fixed at both ends of the column tube, the inner diameter of the tube head is larger than the inner diameter of the column tube, a detachable column head cover is fixedly sleeved on the surface of the tube head, the outer wall of the tube head is provided with a clamping assembly for fixing the column head cover, a sieve plate and a blade ring are clamped inside the tube head, the sieve plate is located close to one side of the column tube, rubber protective plugs are clamped inside the two column head covers, and ejection assemblies are provided on the side walls at both ends of the column tube.

[0006] Furthermore, the column cap is fixedly connected with a connector at one end away from the pipe head.

[0007] Furthermore, a clamping hole is provided on the side wall of the column cap near the clamping assembly, and the clamping assembly includes a first groove provided on the outer wall of the tube head, and a first spring is fixedly connected to the inner wall of the first groove, and a clamping rod is fixedly connected to the other end of the first spring, and the clamping rod is clamped into the inside of the clamping hole at one end away from the first spring.

[0008] Furthermore, a convex ridge is fixed on the inner side wall of the column cap, and a ridge groove for inserting the convex ridge is formed on the outer side wall of the tube head.

[0009] Furthermore, a sealing ring is fixedly sleeved on the outer wall of the blade ring, and the sealing ring is fitted with the inner wall of the pipe head.

[0010] Furthermore, the ejection assembly includes a second groove opened at the end of the column tube, the inner side wall of the second groove is fixedly connected with a second spring, and the other end of the second spring is in contact with the surface of the screen plate.

[0011] Furthermore, the method for using the liquid chromatography column with replaceable fillers is as follows: when replacing the filler, first squeeze the clamping rod, then remove the column cap from the surface of the tube head, take out the sieve plate and the blade ring, remove the column caps, blade rings and sieve plates at both ends, take out the original filler inside the column tube, add new filler and compact it, and finally install the column cap, blade ring and sieve plate. The two column caps of the present invention can be quickly fixed to the surfaces of the two tube heads through the clamping assembly, and the sieve plate and blade ring inside the tube head can be taken out after the column caps are removed, so as to facilitate the rapid replacement of the filler in the column tube.

[0012] Beneficial effects of the present invention: When the present invention is in use, the liquid chromatography column with replaceable filler is provided with a column tube, two tube heads, two column head covers, a clamping assembly, a sieve plate, a blade ring and a rubber protective plug. The two tube heads are located at both ends of the column tube and are an integral structure with the column tube. The two column head covers can be quickly fixed to the surfaces of the two tube heads through the clamping assembly. Therefore, when the column head covers are removed, the sieve plate and the blade ring inside the tube head can be taken out, thereby facilitating the rapid replacement of the filler in the column tube.

[0013] When the present invention is in use, the liquid chromatography column with replaceable filler is provided with a column tube, and both ends of the column tube are provided with ejection assemblies. When the column head cover is removed from the tube head surface at both ends of the column tube, the second spring of the ejection assembly can directly eject the sieve plate and the blade ring from the tube head, thereby making the removal of the sieve plate and the blade ring more convenient and quick. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solution of the present invention, the drawings required for use in the description of the specific implementation methods will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 : An overall cross-sectional view of the present invention; Figure 2 : A partial stereogram of the present invention; Figure 3 :The present invention Figure 1Enlarged view of point A in the middle.

[0016] The reference numerals are as follows: 1. Column tube; 2. Filler; 3. Tube head; 31. Ridge groove; 4. Column head cover; 41. Clamp hole; 42. Raised ridge; 5. Connector; 6. Clamp assembly; 61. First groove; 62. First spring; 63. Clamp rod; 7. Screen plate; 8. Blade ring; 9. Sealing ring; 10. Rubber protective plug; 11. Ejection assembly; 111. Second groove; 112. Second spring. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] like Figure 1-Figure 3 As shown, a liquid chromatography column with replaceable filler comprises a column tube 1, a filler 2 is arranged inside the column tube 1, and tube heads 3 are fixed at both ends of the column tube 1, the inner diameter of the tube head 3 is larger than the inner diameter of the column tube 1, a detachable column head cover 4 is fixedly sleeved on the surface of the tube head 3, the outer wall of the tube head 3 is provided with a clamping assembly 6 for fixing the column head cover 4, a sieve plate 7 and a blade ring 8 are clamped inside the tube head 3, the sieve plate 7 is located on the side close to the column tube 1, rubber protective plugs 10 are clamped inside the two column head covers 4, and ejection assemblies 11 are provided on the side walls at both ends of the column tube 1.

[0019] One end of the column cap 4 away from the pipe head 3 is fixedly connected with a connector 5 .

[0020] In this embodiment, the column tube 1 , the tube head 3 , the column head cover 4 and the connector 5 are all made of stainless steel, and the surface of the connector 5 is provided with threads, so as to facilitate the fixed installation of the chromatographic column through the connector 5 .

[0021] A clamping hole 41 is provided on the side wall of the column cap 4 near the clamping assembly 6. The clamping assembly 6 includes a first groove 61 provided on the outer wall of the tube head 3, and a first spring 62 is fixedly connected to the inner wall of the first groove 61. A clamping rod 63 is fixedly connected to the other end of the first spring 62. The clamping rod 63 is clamped into the inside of the clamping hole 41 at one end away from the first spring 62.

[0022] When installing the column cap 4, first squeeze the clamping rod 63 on the outer wall of the tube head 3 so that the clamping rod 63 retracts into the first groove 61, then put the column cap 4 on the surface of the tube head 3, and align the clamping hole 41 with the clamping rod 63. When the column cap 4 is completely put on the surface of the tube head 3, the clamping hole 41 is aligned with the clamping rod 63. At this time, the first spring 62 squeezes the clamping rod 63 into the clamping hole 41 to fix the column cap 4.

[0023] When removing the column cap 4, first squeeze the clamping rod 63 back into the first groove 61. After the clamping rod 63 is disengaged from the clamping hole 41, the column cap 4 can be pulled off the surface of the pipe head 3, making the installation and removal of the column cap 4 simple and quick.

[0024] A convex ridge 42 is fixed on the inner wall of the column cap 4 , and a ridge groove 31 for clamping the convex ridge 42 is formed on the outer wall of the pipe head 3 .

[0025] When the column cap 4 is sleeved onto the surface of the tube head 3, the convex ridge 42 on the inner wall of the column cap 4 can be aligned with the ridge groove 31 on the outer wall of the tube head 3 and snapped into place. In this way, when the column cap 4 is sleeved on the surface of the tube head 3, the position of the clamping rod 63 and the clamping hole 41 can be just aligned, thereby improving the convenience of installation.

[0026] A sealing ring 9 is fixedly sleeved on the outer wall of the blade ring 8 , and the sealing ring 9 is in contact with the inner wall of the pipe head 3 .

[0027] The sealing ring 9 can increase the sealing between the blade ring 8 and the pipe head 3. When the column head cover 4 is fixed on the surface of the pipe head 3, the column head cover 4 squeezes the blade ring 8, and the blade ring 8 squeezes and fixes the sieve plate 7 inside the pipe head 3.

[0028] The ejection assembly 11 includes a second groove 111 opened at the end of the column tube 1 , and a second spring 112 is fixedly connected to the inner side wall of the second groove 111 , and the other end of the second spring 112 is in contact with the surface of the screen plate 7 .

[0029] When the column cap 4 is removed, the blade ring 8 and the sieve plate 7 lose the compression of the column cap 4 , and the second spring 112 can push the blade ring 8 and the sieve plate 7 out of the pipe head 3 , thereby realizing the rapid removal of the blade ring 8 and the sieve plate 7 .

[0030] The method of using a liquid chromatography column with replaceable filler is as follows: when the filler 2 needs to be replaced, first squeeze the clamping rod 63, then remove the column cap 4 from the surface of the tube head 3, at this time the second spring 112 pushes the blade ring 8 and the sieve plate 7 out of the tube head 3, then take out the sieve plate 7 and the blade ring 8, after the column caps 4, the blade ring 8 and the sieve plate 7 at both ends are removed, take out the original filler 2 inside the column tube 1, then install the column cap 4, the blade ring 8 and the sieve plate 7 at one end of the column tube 1, then add new filler 2 into the column tube 1, and compact the filler 2, and finally install the column cap 4, the blade ring 8 and the sieve plate 7 at the other end of the column tube 1.

[0031] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A liquid chromatography column with replaceable filler, comprising a column tube (1), characterized in that: The column tube (1) is provided with a filler (2) inside, and a tube head (3) is fixed at both ends of the column tube (1), the inner diameter of the tube head (3) is larger than the inner diameter of the column tube (1), a detachable column head cover (4) is fixedly sleeved on the surface of the tube head (3), the outer wall of the tube head (3) is provided with a clamping assembly (6) for fixing the column head cover (4), a sieve plate (7) and a blade ring (8) are clamped inside the tube head (3), the sieve plate (7) is located on a side close to the column tube (1), rubber protective plugs (10) are clamped inside the two column head covers (4), and ejection assemblies (11) are provided on the side walls at both ends of the column tube (1).

2. The liquid chromatography column with replaceable filler according to claim 1, characterized in that: The column cap (4) has one end away from the pipe head (3) fixedly connected to a connector (5).

3. The liquid chromatography column with replaceable filler according to claim 1, characterized in that: A clamping hole (41) is provided on the side wall of the column cap (4) near the clamping assembly (6); the clamping assembly (6) comprises a first groove (61) provided on the outer wall of the pipe head (3); a first spring (62) is fixedly connected to the inner wall of the first groove (61); a clamping rod (63) is fixedly connected to the other end of the first spring (62); and an end of the clamping rod (63) away from the first spring (62) is clamped into the inside of the clamping hole (41).

4. The liquid chromatography column with replaceable filler according to claim 1, characterized in that: A convex ridge (42) is fixed to the inner side wall of the column cap (4), and a ridge groove (31) for snapping into the convex ridge (42) is formed on the outer side wall of the tube head (3).

5. The liquid chromatography column with replaceable filler according to claim 1, characterized in that: A sealing ring (9) is fixedly sleeved on the outer side wall of the blade ring (8), and the sealing ring (9) is in contact with the inner side wall of the pipe head (3).

6. The liquid chromatography column with replaceable filler according to claim 1, characterized in that: The ejection assembly (11) comprises a second groove (111) formed at the end of the column tube (1), a second spring (112) being fixedly connected to the inner side wall of the second groove (111), and the other end of the second spring (112) being in contact with the surface of the screen plate (7).

7. The liquid chromatography column with replaceable filler according to claim 1, wherein the method of using the column is as follows: first, the clamping rod (63) is squeezed, and then the column cap (4) is removed from the surface of the tube head (3), at which time the second spring (112) pushes the blade ring (8) and the sieve plate (7) out of the tube head (3), and then the sieve plate (7) and the blade ring (8) are taken out. After the column caps (4), the blade ring (8) and the sieve plate (7) at both ends are removed, the original filler (2) inside the column tube (1) is taken out, and then the column cap (4), the blade ring (8) and the sieve plate (7) are first installed at one end of the column tube (1), and then new filler (2) is added into the column tube (1), and the filler (2) is compacted, and finally the column cap (4), the blade ring (8) and the sieve plate (7) at the other end of the column tube (1) are installed.

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