Micro-contact transfer printing method based on cell knapsack

Abstract

The invention provides a micro-contact transfer printing method based on a cell knapsack, which comprises the following steps: S1, coating a glass plate with an organic adhesion layer, and heating and drying to obtain an adhesion material; s2, the glass plate is placed on a heating table to be heated, and the seal spin-coated with the cell knapsack adheres to the side, containing the adhesive material, of the glass plate; s3, applying a certain intensity of pressure to the seal, rubbing the cell knapsack, and enabling the cell knapsack to be in conformal contact with the adhesive material; and S4, breaking and separating the seal and the cell knapsack in a stripping manner, and leaving the regular cell knapsack on the adhesive material, thereby completing the micro-contact transfer printing of the cell knapsack. The micro-contact transfer printing method based on the cell knapsack is simple in process, low in operation difficulty, capable of reducing personal errors, convenient to popularize and apply and expected to be used for large-scale production and manufacturing.

Description

Technical Field

[0001] The invention relates to the field of graphite material sealing, and in particular to a micro-contact transfer method based on a cell backpack. Background Art

[0002] Transfer printing is an emerging technology for micro-manufacturing and nano-manufacturing. It is a technology that uses a soft and elastic polymer stamp to transfer the required material from one substrate to another. It can be applied to many fields such as biomedicine, electronic manufacturing, and material science. At the same time, transfer printing technology is highly flexible and extensible, and can be processed on substrates of different materials, sizes, and shapes. By changing the transfer printing into micron-scale micro-contact transfer technology, this technology can be applied to higher-precision devices. Compared with transfer printing technology, it has the advantages of high precision, small size and large quantity. Micro-contact transfer technology is a technology used to create micron-scale patterns on solid surfaces. In the past, there have always been uncertainties in the microcontact transfer technology of cell backpacks, so the improvement of transfer technology has been a major challenge. In traditional microcontact transfer technology, it is necessary to directly contact the cell backpack stamp with a glass plate coated only with polyvinyl alcohol on a heating plate, place a certain weight of weight for a period of time, and then peel off the cell backpack stamp; or, place the polyvinyl alcohol-coated glass plate on a water bath and keep it for a period of time, and then directly contact the cell backpack stamp with the glass plate. This leads to the instability of the microcontact transfer efficiency of the cell backpack and consumes more raw materials. The emergence of microcontact transfer optimization technology has improved work efficiency to a certain extent and alleviated the severe trend. Elastic stamp material PDMS is the most commonly used elastic stamp material in microcontact transfer technology. It has good elasticity and flexibility, and can be in close contact with the substrate surface without damaging the substrate. It is biocompatible and degradable, and is widely used in the biomedical field. PDMS can also be prepared into stamps of various shapes and sizes through simple photolithography technology to meet different transfer needs. Self-assembled monolayer technology is one of the important foundations of microcontact transfer technology. Self-assembled monolayer is an ordered monolayer structure spontaneously formed by molecules on the surface of a substrate through chemical bonds or physical adsorption. The surface of the material is modified and regulated at the molecular level, with high selectivity and controllability. Through this technology, specific chemical functional groups or patterns can be formed on the surface of the substrate, providing a good template for microcontact transfer. Surface science has conducted in-depth research on the physical and chemical properties of material surfaces, providing a theoretical basis for microcontact transfer technology. For example, research on surface energy, wettability, and interactions can further optimize the transfer process. By improving the transfer technology, the transfer efficiency of cell backpacks can be improved to a higher degree. Therefore, it is necessary to invent a new microcontact transfer method based on cell backpacks to solve the above problems. Summary of the invention

[0003] Technical problem to be solved: The purpose of the present invention is to provide a microcontact transfer technology with good adhesion performance, high chemical stability, firm adhesion to the surface of the transferred material, and simple steps, so as to optimize the current microcontact transfer method of cell backpacks.

[0004] Technical solution: A microcontact transfer method based on a cell backpack, comprising the following steps: S1. The organic adhesive layer is coated on a glass plate, heated and dried to obtain an adhesive material; S2. Place the glass plate on a heating table for heating, and adhere the stamp with the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S3. applying a certain pressure to the stamp to imprint the cell backpack, so that the cell backpack is in conformal contact with the adhesion material; S4. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Preferably, the organic adhesive coating in step S1 is a mixture of PVA, a hydroxyl-containing polar solvent and water, wherein the content of PVA in the mixture is 2.5-3.5wt%, and the content of the hydroxyl-containing polar solvent is 5-9wt%. Preferably, the hydroxyl-containing polar solvent is ethylene glycol. Preferably, the heating temperature in step S2 is 60-70°C. Preferably, the pressure in step S3 is 3200.0Pa-5500.0Pa, and the time for applying the pressure is 50-70s. Preferably, the stamp is a PDMS protrusion array mold, and the diameter, height and spacing of the cylindrical protrusions on the PDMS protrusion array mold are all 3-15 μm. Preferably, the material of the cell backpack includes a support layer and an adhesion layer, the support layer is polylactic acid-glycolic acid copolymer, the adhesion layer is a mixture of polyallylamine hydrochloride and hyaluronic acid aldehyde, and the adhesion layer and the support layer are sequentially spin-coated on the surface of the PDMS protrusion array mold having a periodically arranged micrometer-scale microcolumn matrix structure. Beneficial effects: The micro-contact transfer method of the present invention has the following advantages: 1. The organic coating selected in the present invention is a mixed solution of PVA and ethylene glycol. Ethylene glycol is an organic compound that is miscible with water and acetone, and has a certain solubility in ethers. In terms of chemical properties, ethylene glycol is relatively stable. It contains two hydroxyl groups, which makes it very hydrophilic. The hydroxyl groups in the PVA aqueous solution form hydrogen bonds with the polar solvent ethylene glycol, making the interaction between molecules closer and increasing the viscosity of the PVA aqueous solution. After heating, the movement of the PVA molecular chains is intensified, and ethylene glycol acts as a plasticizer. It can be inserted between PVA molecular chains, causing changes in the forces between the molecular chains, increasing the flexibility and fluidity of the molecular chains to a certain extent, and allowing the PVA to better entangle with each other in a heated state, thereby showing stronger viscosity. 2. The present invention adds ethylene glycol to the original PVA organic adhesive coating, so that the original method requiring water bath heating is further improved, the temperature and water are quantitatively controlled, the current transfer efficiency is improved, and a higher success rate of backpack transfer is guaranteed, and the transfer efficiency is maintained at 80%-90%, which prevents a large number of backpack losses in the past, so as to promote further experiments; 3. The microcontact transfer technology based on the cell backpack of the present invention has a relatively simple process, reduces the difficulty of operation, reduces human errors, is easy to promote and apply, and is expected to be used for large-scale production; 4. The process of the present invention can be used for transfer operations between a variety of materials, and has strong scalability; and the present invention has strong repeatability and can be reproduced many times. The time required for each transfer is very short, which can greatly improve work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 It is a front view structural schematic diagram of a cell backpack stamp coated with an organic adhesive coating in the present invention; Figure 2 It is a schematic diagram of a top view of a backpack adhered to an organic adhesive coating after micro-contact transfer in the present invention; Figure 3 It is a front view structural schematic diagram of a backpack adhered to the organic adhesive coating after micro-contact transfer in the present invention; Figure 4 It is a schematic diagram of a stamp printed on an organic adhesive coating in the present invention; Figure 5 Schematic diagram of the entire micro-contact transfer process of the present invention; Figure 6 Fluorescence microscopy images of cell backpacks transferred onto an organic adhesive coating; Figure 7 The fluorescence microscopy images of the transfer onto the organic adhesive coating at different heating temperatures in Comparative Example 1 and Comparative Example 2, wherein A is the fluorescence microscopy image of Comparative Example 1; B is the fluorescence microscopy image of Comparative Example 2; Figure 8The fluorescence microscopy images of the organic adhesive coating transferred at different applied pressures in Comparative Example 3 and Comparative Example 4, wherein A is the fluorescence microscopy image of Comparative Example 3; B is the fluorescence microscopy image of Comparative Example 4; Fig. 9 The fluorescence microscope images of the organic adhesive coating transferred at different times in Comparative Example 5 and Comparative Example 6, wherein A is the fluorescence microscope image of Comparative Example 5; B is the fluorescence microscope image of Comparative Example 6; Fig.10 The fluorescence microscopy images of the organic adhesive coatings transferred to the organic adhesive coatings in Comparative Examples 7 and 8 at different concentrations of ethylene glycol in the same organic adhesive coatings, wherein A is the fluorescence microscopy image of Comparative Example 7; B is the fluorescence microscopy image of Comparative Example 8; Fig.11 The fluorescence microscopy images of the organic adhesive coatings transferred onto the organic adhesive coatings when different organic adhesive coating materials are used in Comparative Examples 9-12, wherein A is a fluorescence microscopy image of the organic adhesive coating having only PVA; B is a fluorescence microscopy image of the organic adhesive coating having propylene glycol; C is a fluorescence microscopy image of the organic adhesive coating having butylene glycol; and D is a fluorescence microscopy image of the organic adhesive coating having glycerol; Fig.12 The comparison of the number of transferred cell backpacks at different concentrations of ethylene glycol in the same organic adhesive coating when other transfer conditions are the same; Fig.13 This is a comparison chart of the number of transferred cell backpacks at different heating temperatures when other transfer conditions are the same; Fig.14 This is a comparison chart of the number of transferred cell backpacks when other transfer conditions are the same and different applied pressures are applied; Fig.15 This is a comparison chart of the number of transferred cell backpacks at different transfer times when other transfer conditions are the same; Fig.16 The comparison chart of the number of transferred cell backpacks with different organic adhesive coatings when other transfer conditions are the same; In the figure, 1-glass plate; 2-cell backpack stamp; 3-weight; 4-heating table; 5-organic adhesive coating; 6-cell backpack. DETAILED DESCRIPTION The present invention is further described below in conjunction with the embodiments and drawings of the specification. The following embodiments are explanations of the present invention and the present invention is not limited to the following embodiments: Reference Figure 1 , Figure 2 and Figure 3 The cell backpack stamp 2 in the microcontact transfer method has a periodically arranged micron-scale micro-column matrix structure on its surface, and the force between the micro-micro-columns and the organic adhesive coating 5 on the surface of the glass plate 1 is sufficient to adhere the cell backpack 6 to the surface of the organic adhesive coating 5 on the glass plate 1. Example 1 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix uniformly to obtain an organic adhesion layer, wherein the content of PVA was 2.5wt% and the content of ethylene glycol was 5wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 60°C, and adhere the spin-coated cell backpack stamp to the side of the glass plate containing the adhesive material; S4. applying a pressure of 3200.0 Pa to the stamp for 70 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Example 2 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix evenly to obtain an organic adhesion layer, wherein the content of PVA was 3.5wt% and the content of ethylene glycol was 9wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 70°C, and adhere the spin-coated cell backpack stamp to the side of the glass plate containing the adhesive material; S4. applying a pressure of 5500.0 Pa to the stamp for 50 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Example 3 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to obtain an organic adhesion layer, wherein the content of PVA was 2.8wt% and the content of ethylene glycol was 6wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 65°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. applying a pressure of 3800.0 Pa to the stamp for 55 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Example 4 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix uniformly to obtain an organic adhesion layer, wherein the content of PVA was 3.2wt% and the content of ethylene glycol was 7wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 65°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. Applying a pressure of 4500.0 Pa to the stamp for 60 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Comparative Example 1 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix uniformly to obtain an organic adhesion layer, wherein the content of PVA was 3.2wt% and the content of ethylene glycol was 7wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 50°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. Applying a pressure of 4500.0 Pa to the stamp for 60 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Comparative Example 2 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix uniformly to obtain an organic adhesion layer, wherein the content of PVA was 3.2wt% and the content of ethylene glycol was 7wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 80°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. Applying a pressure of 4500.0 Pa to the stamp for 60 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Comparative Example 3 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix uniformly to obtain an organic adhesion layer, wherein the content of PVA was 3.2wt% and the content of ethylene glycol was 7wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 65°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. applying a pressure of 2500.0 Pa to the stamp for 60 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Comparative Example 4 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix uniformly to obtain an organic adhesion layer, wherein the content of PVA was 3.2wt% and the content of ethylene glycol was 7wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 65°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. Apply a pressure of 6500.0 Pa to the stamp for 60 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Comparative Example 5 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix uniformly to obtain an organic adhesion layer, wherein the content of PVA was 3.2wt% and the content of ethylene glycol was 7wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 65°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. Apply a pressure of 4500.0 Pa to the stamp for 30 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Comparative Example 6 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix uniformly to obtain an organic adhesion layer, wherein the content of PVA was 3.2wt% and the content of ethylene glycol was 7wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 65°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. Applying a pressure of 4500.0 Pa to the stamp for 90 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Comparative Example 7 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to mix uniformly to obtain an organic adhesion layer, wherein the content of PVA was 3.2wt% and the content of ethylene glycol was 2wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 65°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. Applying a pressure of 4500.0 Pa to the stamp for 60 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Comparative Example 8 A microcontact transfer method based on a cell backpack comprises the following steps: S1. PVA was dissolved in deionized water and mixed thoroughly in a magnetic stirring water bath at 60°C, and then ethylene glycol was added to the solution and stirred to obtain an organic adhesion layer, wherein the content of PVA was 3.2wt% and the content of ethylene glycol was 11wt%; S2. The organic adhesive layer is coated on a glass plate and heated and dried at 60° C. for 1 h to obtain an adhesive material; S3. Place the glass plate on a heating table and heat it at 65°C, and adhere the stamp of the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S4. Applying a pressure of 4500.0 Pa to the stamp for 60 seconds to print the cell backpack so that the cell backpack is in conformal contact with the adhesion material; S5. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack. Comparative Example 9 The difference between Comparative Example 9 and Example 4 is that in step S1, only PVA is used as the organic adhesion layer; Comparative Example 10 The difference between Comparative Example 10 and Example 4 is that the ethylene glycol in step S1 is replaced by propylene glycol; Comparative Example 11 The difference between Comparative Example 11 and Example 4 is that the ethylene glycol in step S1 is replaced by butanediol; Comparative Example 12 The difference between Comparative Example 12 and Example 4 is that the ethylene glycol in step S1 is replaced by propylene glycol. Transfer efficiency Transfer efficiency Transfer efficiency Transfer efficiency Example 1 82.3％ Comparative Example 1 69.7％ Comparative Example 7 49.3％ Example 2 85.5％ Comparative Example 2 5.3％ Comparative Example 8 67.7％ Example 3 85.8％ Comparative Example 3 45.7％ Comparative Example 9 20.7％ Example 4 89.7％ Comparative Example 4 2.0％ Comparative Example 10 39.0％ Comparative Example 5 37.3％ Comparative Example 11 44.3％ Comparative Example 6 1.3％ Comparative Example 12 1.3％ Further explanation is given with reference to the embodiments and the accompanying drawings. Figure 7 It can be seen from the figure that when the heating temperatures are different in Example 4 and Comparative Example 1, A is the transfer effect of Comparative Example 1. When the transfer temperature is insufficient, only the center point of each backpack can be transferred; B is the transfer effect of Comparative Example 2. When the transfer temperature is high, the cell backpack material of each cell backpack will be oxidized, and the transferred result will have patterns and be uneven. Fig.13 The figure is a comparison data of the transfer efficiency of the transfer cell backpack at different transfer temperatures. from Figure 8 It can be seen that A is the transfer effect of comparative example 3. When the applied pressure is insufficient, some cell backpacks can be transferred, while most of the cell backpacks can only transfer a central point or even fail to transfer; B is the transfer effect of comparative example 4. When the applied pressure is too high, the cell backpack material in the middle of the protrusion of the cell backpack stamp will also be fully transferred, resulting in the entire piece being torn off. Fig.14 The figure shows the transfer efficiency comparison of the cell backpack at different applied pressures. Fig. 9 It can be seen that A is the transfer effect of comparative example 5. When the transfer time is too short, only the center point of the cell backpack can be transferred, and part of it is not transferred at all; B is the transfer effect of comparative example 6. When the transfer time is too long, the cell backpack material in the middle of the protrusion of the cell backpack stamp will also be fully transferred, resulting in the entire piece being torn off. Fig.15 The figure shows the comparison of transfer efficiency of the transfer cell backpack at different transfer times. from Fig.10It can be seen that A is the transfer effect of comparative example 7. When the ethylene glycol concentration is reduced, a part of the complete cell backpack can be transferred, and some parts are not transferred; B is the transfer effect of comparative example 8. When the ethylene glycol concentration is increased, a part of the complete cell backpack can be transferred, and some parts are not transferred. Fig.12 The figure is a comparison data of the transfer efficiency of the transfer cell backpack at different concentrations of ethylene glycol. from Fig.11 It can be seen from the figure that the transfer efficiency in comparative examples 9-12 is that A in the figure is the transfer effect of the organic adhesive coating only PVA. At this time, only a small part of the center point of the cell backpack can be transferred; B is the transfer effect of the organic adhesive coating being propylene glycol. At this time, a part of the center point of the cell backpack can be transferred; C is the transfer effect of the organic adhesive coating being butanediol. At this time, almost half of the center point of the cell backpack can be transferred; D is the transfer effect of the organic adhesive coating being propylene glycol. The cell backpack material of the cell backpack will be chemically modified, and the transfer will be patterned and uneven. Fig.16 The figure shows the comparison of transfer efficiency of cell backpacks with different organic adhesive coatings. In the traditional micro-contact transfer technology, it is necessary to directly contact the cell backpack stamp with the glass plate coated with PVA on a heating plate at 50°C, place a certain weight of weight and keep it for 10 seconds, and then peel off the cell backpack stamp; or, place the PVA-coated glass plate on a 60°C water bath and keep it for 5 seconds, and then directly contact the cell backpack stamp with the glass plate. This leads to the instability of the micro-contact transfer efficiency of the cell backpack and consumes more raw materials. The present invention adds 5-9% ethylene glycol on the basis of the original organic adhesive coating, so that the original method that requires water bath heating is further improved, and the temperature and water are quantitatively achieved. Compared with the addition of other organic solutions, the current transfer efficiency can be better improved, and a higher success rate of backpack transfer can be guaranteed. The transfer efficiency is maintained at 80%-90%, and a large amount of previous backpack losses are prevented to promote further experiments. The emergence of micro-contact transfer optimization technology has improved work efficiency to a certain extent and alleviated the severe trend. The above-mentioned microcontact transfer technology based on cell backpacks has expanded the application scope of microcontact transfer technology and provided the possibility of using transfer technology for more devices and materials. The above is a detailed introduction to the optimization method of the microcontact transfer technology based on the cell backpack provided by the present invention. Specific examples are used in this article to illustrate the structure and working principle of the present invention. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. The above is only a preferred embodiment of the present invention and is not intended to limit the present invention. The above embodiments are only examples for clear explanation and are not limitations on the implementation methods. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A microcontact transfer method based on a cell backpack, characterized in that: The following steps are involved: S1. The organic adhesive layer is coated on a glass plate, heated and dried to obtain an adhesive material; S2. Place the glass plate on a heating table for heating, and adhere the stamp with the spin-coated cell backpack to the side of the glass plate containing the adhesive material; S3. applying a certain pressure to the stamp to imprint the cell backpack, so that the cell backpack is in conformal contact with the adhesion material; S4. The stamp and the cell backpack are broken and separated by peeling, leaving the regular cell backpack on the adhesive material, thus completing the microcontact transfer of the cell backpack.

2. The microcontact transfer method based on cell backpack according to claim 1, characterized in that: In the step S1, the organic adhesive coating is a mixture of PVA, a hydroxyl-containing polar solvent and water, wherein the content of PVA in the mixture is 2.5-3.5wt%, and the content of the hydroxyl-containing polar solvent is 5-9wt%.

3. The microcontact transfer method based on cell backpack according to claim 2, characterized in that: The hydroxyl-containing polar solvent is ethylene glycol.

4. The microcontact transfer method based on cell backpack according to claim 1, characterized in that: The heating temperature in step S2 is 60-70°C.

5. The microcontact transfer method based on cell backpack according to claim 1, characterized in that: The pressure in step S3 is 3200.0Pa-5500.0Pa, and the pressure is applied for 50-70s.

6. The microcontact transfer method based on cell backpack according to claim 1, characterized in that: The stamp is a PDMS protrusion array mold, and the diameter, height and spacing of the cylindrical protrusions on the PDMS protrusion array mold are all 3-15 μm.

7. The microcontact transfer method based on cell backpack according to claim 6, characterized in that: The material of the cell backpack includes a support layer and an adhesion layer, wherein the support layer is a polylactic acid-glycolic acid copolymer, and the adhesion layer is a mixture of polyallylamine hydrochloride and hyaluronic acid aldehyde. The adhesion layer and the support layer are sequentially spin-coated on a periodically arranged micron-scale microcolumn matrix structure on the surface of a PDMS protrusion array mold.

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