Reactor for cell culture and use method thereof

Abstract

The invention relates to the technical field of cell culture, and discloses a reactor for cell culture and a use method thereof. The reactor for cell culture comprises a machine body, a culture assembly is arranged, after culture medium liquid and cell bodies are input into a reaction cylinder, the top end of a center rod is clamped to the bottom end of an accelerator, the outer side of a sliding cylinder is sleeved with the top end of a conical cover, and a servo motor, a matching round frame, an N-shaped frame, a linear module, a sliding block and a limiting rod are started; a conical cover can be clamped and fixed in cooperation with a three-jaw hydraulic chuck, clamping jaws and a silica gel ring, the oxygen content in the reaction cylinder is obtained in cooperation with a dissolved oxygen sensor, the amount of oxygen entering the reaction cylinder is changed in cooperation with vertical movement of a controller, a top end electric push rod, a piston rod, a sliding cylinder and a trapezoidal groove, and the cell culture effect can be better; and the operation is more automatic.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a reactor for cell culture and a use method thereof. Background Art

[0002] With the increasing requirements for cell culture, cell culture is a basic technology for research in many fields such as biology and medicine, and is widely used in drug development, cell therapy, vaccine production, etc. In drug development, cell culture is needed to study the mechanism of drug action on cells and screen effective drug ingredients. Traditional cell culture methods such as culture bottles and culture dishes have problems such as cumbersome operation, small scale, and difficulty in accurately controlling culture conditions, and cannot meet the needs of large-scale, high-quality cell culture.

[0003] However, in some reactors, it is still difficult to avoid uneven distribution of temperature, dissolved oxygen concentration and nutrients in local areas, which will lead to inconsistent cell growth rates, affect cell quality and yield, and restrict cell growth. There is a lack of stirring mechanism and mechanism for adjusting oxygen input. The effect of cell culture needs to be improved, and the operation automation needs to be improved. Summary of the invention

[0004] The object of the present invention is to provide a reactor for cell culture and a method of using the same to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A reactor for cell culture, comprising a body, a culture barrel fixedly mounted on one side of the top of the body, a heater arranged on the outer side of the bottom end of the culture barrel, an air inlet pipe and an air outlet pipe fixedly mounted on both sides of the top of the culture barrel, solenoid valves arranged on the air inlet pipe and the air outlet pipe, an oxygen supply device connected to the outer side of the air inlet pipe, an exhaust gas collection device connected to the outer side of the air outlet pipe, a round cover fixedly mounted on the top of the culture barrel, a controller fixedly mounted on the top of the body, a culture component arranged on the top of the body, and the culture component comprising:

[0007] A servo motor is provided on the outside of the machine body, the outside of the output shaft at the bottom of the servo motor is fixedly installed at the inner center of the round frame, an N-shaped frame is fixedly installed at the bottom of the round frame, a linear module is fixedly installed on the inner side of the top of the N-shaped frame, a sliding block is slidably installed on the inner side of the linear module, a limit rod is fixedly installed on the inner side of the N-shaped frame, the sliding block slides and fits on the outer side of the limit rod, a three-jaw hydraulic chuck is fixedly installed at the bottom of the sliding block, a clamping jaw is slidably installed on the three-jaw hydraulic chuck, and a silicone ring is sleeved on the bottom of the clamping jaw;

[0008] A conical cover, the outer side of the silicone ring is clamped with a conical cover, the inner side of the conical cover is threadedly installed with a reaction cylinder, the reaction cylinder is filled with culture medium and cell body, the inner side of the top of the reaction cylinder is clamped with a conical bucket, the arc-shaped inner wall of the conical bucket is fixedly installed with a handle, the outer side of the top of the conical bucket is fixedly installed with a card block, a card slot is opened on the side wall of the top of the reaction cylinder, the card block is attached to the inner side of the card slot, and a dissolved oxygen sensor is clamped inside the arc-shaped side wall of the reaction cylinder;

[0009] A top electric push rod is fixedly installed at the bottom center of the three-jaw hydraulic chuck, a piston rod is slidably installed on the inner side of the top electric push rod, the bottom of the piston rod is fixedly connected to the top center of the sliding cylinder, and a trapezoidal groove is opened on the arc-shaped side wall of the sliding cylinder.

[0010] Preferably, the N-shaped frame, linear module, sliding block, limit rod and three-jaw hydraulic chuck are provided in multiple groups, and the multiple groups of N-shaped frames, linear modules, sliding blocks, limit rods and three-jaw hydraulic chucks are all arranged in a circular array with equal spacing with the center of the circular cross-section of the circular frame as the center of the array, and the clamping jaws and silicone rings on a single group of the three-jaw hydraulic chuck are provided with three groups, so as to better clamp and fix.

[0011] Preferably, the trapezoidal grooves are provided in a plurality of groups, and the top side length of the vertical cross section of the trapezoidal groove is smaller than the bottom side length thereof.

[0012] Preferably, a mixing assembly is provided on the reaction cylinder, and the mixing assembly includes a bottom electric push rod, and the bottom of the reaction cylinder is fixedly installed with the bottom electric push rod, and a sealing bearing and a sealing rubber ring are provided on the outer side of the piston end of the bottom electric push rod, and the sealing bearing and the sealing rubber ring are fixedly installed on the inner wall of the bottom end of the reaction cylinder, and the sealing bearing is located below the sealing rubber ring, and a spring is fixedly installed on the top of the piston end of the bottom electric push rod, and one end of a pressure sensor is fixedly installed on the inner side of the piston end of the bottom electric push rod, and the other end of the pressure sensor is attached to the bottom of the spring, so that the pressure sensor can better obtain the pressure data received.

[0013] Preferably, a reduction motor is fixedly installed at the bottom center of the reaction barrel, a rotating shaft is fixedly installed at the output end of the reduction motor, a center rod is fixedly installed on the top of the rotating shaft, a sealed bearing and a sealing rubber ring are also provided on the outer side of the bottom end of the center rod, a circular ring is fixedly installed on the outer side of the center rod, a stirring column is fixedly installed on the outer side of the circular ring, and a fixing ring is fixedly installed on the bottom of the reaction barrel. The fixing ring is located on the outer side of the bottom electric push rod and the reduction motor, and cooperates with the fixing ring to better place the reaction barrel when not in use.

[0014] Preferably, the reaction cylinders are provided in multiple groups, and the inner side of a single group of the reaction cylinders is provided with multiple groups of circular rings, and the multiple groups of circular rings are arranged in a linear array with equal spacing, and the stirring columns on a single group of the circular rings are provided with multiple groups, and the outer side of the stirring columns has an arc-shaped outer wall to avoid shear force generated by stirring and damage to the cell body.

[0015] Preferably, a rotating assembly is provided on the inner side of the conical cover, and the rotating assembly includes a support rod, and the arc-shaped inner wall of the conical bucket is fixedly mounted with the support rod, and the support rod is provided in multiple groups, and accelerators are fixedly mounted between the multiple groups of support rods, the top end of the center rod is clamped on the bottom input end of the accelerator, and a rotating rod is fixedly mounted on the top output end of the accelerator, and a turbine is fixedly mounted on the outside of the rotating rod.

[0016] Preferably, a sealing assembly is provided on the outside of the conical cover, and the sealing assembly includes a top tube, a top tube is fixedly installed on the bottom of the sliding block, the top tube is located on the outside of the three-jaw hydraulic chuck, an arc ring is fixedly installed on the bottom of the top tube, a rubber ring is fixedly installed on the inside of the round cover, a coil spring is provided inside the rubber ring, and the outer side of the top tube fits against the inner side of the rubber ring, thereby achieving a better sealing effect.

[0017] Preferably, a moving component is provided at the top of the body, and the moving component includes a frame, a frame is fixedly installed on the other side of the top of the body, a vertical hydraulic cylinder is fixedly installed on the top of the frame, a cross plate is fixedly installed on the piston end of the vertical hydraulic cylinder, a sliding sleeve is fixedly installed inside the cross plate, a sliding rod is fixedly installed inside the frame, and the sliding sleeve is slidably fitted on the outside of the sliding rod, and a horizontal hydraulic cylinder is fixedly installed on the bottom of the cross plate, and a U-shaped frame is fixedly installed on the piston end of the horizontal hydraulic cylinder, an asynchronous motor is fixedly installed on the outside of the U-shaped frame, a threaded rod is fixedly installed on the outside of the asynchronous motor output shaft, the threaded rod is rotatably installed on the inside of the U-shaped frame, a threaded block is threadedly installed on the outside of the threaded rod, and the bottom of the threaded block is fixedly connected to the top center of the servo motor, a sliding block is fixedly installed on the outside of the threaded block, a slide rail is fixedly installed on the inside of the U-shaped frame, and the slider is slidably installed on the inside of the slide rail, thereby improving the automation degree of the device body and facilitating operation.

[0018] The present invention also provides a method for using a reactor for cell culture, comprising the following steps:

[0019] S1. After the culture medium and the cell bodies are input into the reaction cylinder, the block is inserted into the slot with the handle, so that the conical bucket can be inserted into the inner side of the reaction cylinder. Then the reaction cylinder is screwed into the conical cover, and the top of the center rod is inserted into the bottom of the accelerator, and the top of the conical cover is sleeved on the outer side of the sliding cylinder.

[0020] S2. When the servo motor is started through the controller, the round frame rotates, and the linear module on the N-shaped frame is started, so that the sliding block moves outside the limit rod, thereby causing the three-jaw hydraulic chuck to move. When the three-jaw hydraulic chuck is started, it can clamp and fix the conical cover with multiple groups of clamping jaws and silicone rings;

[0021] S3, start the vertical hydraulic cylinder on the frame through the controller, cooperate with the cross plate, sliding sleeve and sliding rod to make the horizontal hydraulic cylinder move up and down, start the horizontal hydraulic cylinder to make the U-shaped frame move left and right, start the asynchronous motor to make the threaded rod rotate, cooperate with the threaded block, sliding block and sliding rail to make the servo motor multi-axis movement;

[0022] S4, by aligning the top tube with the rubber ring, the arc ring moves downward to squeeze the rubber ring, causing the coil spring to shrink and deform, thereby achieving a better sealing effect. At this time, the reaction tube as a whole enters the interior of the culture barrel;

[0023] S5. Cooperate with the heater to control the temperature inside the culture barrel, and cooperate with the air inlet pipe, air outlet pipe and solenoid valve to input oxygen into the inside of the culture barrel and discharge waste gas;

[0024] S6, cooperate with the dissolved oxygen sensor to obtain the oxygen content inside the reaction tube, thereby transmitting a signal to the controller, starting the top electric push rod, so that the piston rod drives the sliding tube and the trapezoidal groove to move up and down, thereby changing the amount of oxygen entering the reaction tube;

[0025] S7, start the electric push rod at the bottom, cooperate with the sealed bearing and the sealing rubber ring, so that the electric push rod at the bottom drives the spring and the pressure sensor to move up and down, so as to cooperate with the pressure sensor to convert the acquired pressure value into the viscosity of the mixture, so as to cooperate with the controller servo to start the reduction motor, cooperate with the rotating shaft, the center rod, the ring and the stirring column to stir the mixture, so as to achieve better culture effect of the cell body and more convenient operation;

[0026] S8. When the center rod rotates, it cooperates with the support rod, accelerator and rotating rod to make the turbine rotate, thereby better transporting oxygen to the inside of the reaction tube.

[0027] Compared with the prior art, the present invention provides a reactor for cell culture and a method of using the same, which has the following beneficial effects:

[0028] 1. In order to achieve better cell culture effect and more automated operation, a culture component is provided. After the culture medium and cell body are input into the reaction cylinder, the cone bucket is clamped into the inner side of the reaction cylinder with the handle, the block and the slot, and then the reaction cylinder is screwed into the cone cover, and the top of the center rod is clamped to the bottom of the accelerator, and the top of the cone cover is sleeved on the outside of the sliding cylinder. When the servo motor is started by the controller, the round frame rotates, and the three-jaw hydraulic chuck is moved with the N-shaped frame, the linear module, the sliding block and the limit rod. When the three-jaw hydraulic chuck is started, the cone cover can be clamped and fixed with the clamping claw and the silicone ring, and the oxygen content inside the reaction cylinder is obtained with the dissolved oxygen sensor, so as to transmit the signal to the controller, and the top electric push rod is started, so that the piston rod drives the sliding cylinder and the trapezoidal groove to move up and down, thereby changing the amount of oxygen entering the reaction cylinder, which can achieve better cell culture effect and more automated operation.

[0029] 2. In order to achieve better culture effect of the cell body and more convenient operation, a mixing component is provided, the bottom electric push rod is started, and the sealed bearing and the sealing rubber ring are matched, so that the bottom electric push rod drives the shrapnel and the pressure sensor to move up and down, so as to cooperate with the pressure sensor to convert the acquired pressure value into the viscosity of the mixture, so as to cooperate with the controller servo to start the reduction motor, and cooperate with the rotating shaft, center rod, ring and stirring column to stir the mixture, so as to achieve better culture effect of the cell body and more convenient operation.

[0030] 3. In order to provide sufficient oxygen for the respiration and growth of cells and ensure the smooth progress of the cell culture process, a rotating assembly is provided. When the central rod rotates, the power generated by it is transmitted to the accelerator on the support rod. The accelerator enhances the power, so that the rotating rod rotates faster. The high-speed rotation of the rotating rod drives the turbine connected to it to rotate synchronously. The rotation of the turbine can better transport oxygen to the inside of the reaction cylinder, thereby providing sufficient oxygen for the respiration and growth of cells and ensuring the smooth progress of the cell culture process.

[0031] 4. In order to enhance the sealing effect, a sealing component is provided, the top tube is accurately aligned with the rubber ring, and a certain external force is applied to make the arc ring move slowly downward, gradually squeezing the rubber ring. The rubber ring undergoes elastic deformation under the force, and the coil spring also contracts and deforms accordingly. Through such structural design and operation, the sealing effect is enhanced. At this time, the reaction tube as a whole smoothly enters the interior of the culture barrel.

[0032] 5. In order to meet different operational requirements, a moving component is provided. The vertical hydraulic cylinder on the frame is started by the controller, and the horizontal hydraulic cylinder moves up and down in conjunction with the cross plate, sliding sleeve and sliding rod. The threaded rod rotates in conjunction with the U-shaped frame and asynchronous motor. The servo motor moves in multiple axes in conjunction with the threaded block, sliding block and sliding rail, thereby meeting different operational requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a schematic top view of the overall structure of the present invention;

[0034] Figure 2 It is a schematic side view of the overall structure of the present invention from another viewing angle;

[0035] Figure 3 For the present invention Figure 2 A schematic diagram of the enlarged structure of the middle A area;

[0036] Figure 4 It is a schematic bottom view of a partial structural section of the present invention;

[0037] Figure 5 It is a bottom view schematic diagram of a part of the structure of the present invention;

[0038] Figure 6 It is a schematic diagram of a cross-sectional bottom view of the rubber ring of the present invention;

[0039] Figure 7 It is a schematic top view of part of the structure of the present invention;

[0040] Figure 8 It is a schematic cross-sectional view of the top tube and some structures of the present invention;

[0041] Fig. 9 It is a schematic bottom view of the cone cover and some structures of the present invention;

[0042] Fig.10 For the present invention Fig. 9 Schematic diagram of the enlarged structure of the middle B area;

[0043] Fig.11 It is a schematic cross-sectional view of the reaction tube and some structures of the present invention;

[0044] Fig.12 It is a schematic diagram of a cross-sectional top view of a reaction tube and some structures of the present invention;

[0045] Fig.13 For the present invention Fig.12 Schematic diagram of the enlarged structure of the middle C area;

[0046] Fig.14 For the present invention Fig.12 Schematic diagram of the enlarged structure of the middle D area;

[0047] Fig.15 For the present invention Fig.13 Schematic diagram of the enlarged structure of the middle E area;

[0048] Fig.16 The figure is a flow chart of the method of the present invention.

[0049] In the figure: 1, body; 2, culture barrel; 3, heater; 4, air inlet pipe; 5, air outlet pipe; 6, solenoid valve; 7, round cover; 8, controller; 9, culture component; 91, servo motor; 92, round frame; 93, N-shaped frame; 94, linear module; 95, sliding block; 96, limit rod; 97, three-jaw hydraulic chuck; 98, clamping claw; 99, silicone ring; 901, conical cover; 902, reaction cylinder; 903, conical bucket; 904, handle; 905, block; 906, slot; 907, dissolved oxygen sensor; 908, top electric push rod; 909, piston rod; 9010, sliding cylinder; 9011, trapezoidal groove; 10, mixing component; 101, bottom electric push rod; 102, sealed bearing; 103, sealant ring; 104, spring; 105, pressure sensor; 106, reduction motor; 107, rotating shaft; 108, center rod; 109, ring; 1010, stirring column; 1011, fixed ring; 11, rotating assembly; 111, support rod; 112, accelerator; 113, rotating rod; 114, turbine; 12, sealing assembly; 121, top tube; 122, arc ring; 123, rubber ring; 124, coil spring; 13, moving assembly; 131, frame; 132, vertical hydraulic cylinder; 133, horizontal plate; 134, sliding sleeve; 135, sliding rod; 136, horizontal hydraulic cylinder; 137, U-shaped frame; 138, asynchronous motor; 139, threaded rod; 1310, threaded block; 1311, slider; 1312, slide rail. DETAILED DESCRIPTION

[0050] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0051] See also Figure 1-Figure 16 The present invention provides a technical solution: a reactor for cell culture, comprising a body 1, a culture barrel 2 is fixedly installed on one side of the top of the body 1, a heater 3 is arranged on the outer side of the bottom end of the culture barrel 2, an air inlet pipe 4 and an air outlet pipe 5 are respectively fixedly installed on both sides of the top of the culture barrel 2, and an electromagnetic valve 6 is arranged on the air inlet pipe 4 and the air outlet pipe 5, an oxygen supply device is connected to the outer side of the air inlet pipe 4, and an exhaust gas collection device is connected to the outer side of the air outlet pipe 5, a round cover 7 is fixedly installed on the top of the culture barrel 2, and a controller 8 is fixedly installed on the top of the body 1.

[0052] In one embodiment of the present invention, a culture component 9 is provided at the top of the body 1, and the culture component 9 includes a servo motor 91. The servo motor 91 is provided on the outside of the body 1. The outer side of the output shaft at the bottom end of the servo motor 91 is fixedly installed at the center of the inside of the round frame 92. An N-shaped frame 93 is fixedly installed at the bottom of the round frame 92. A linear module 94 is fixedly installed on the inner side of the top of the N-shaped frame 93. A sliding block 95 is slidably installed on the inner side of the linear module 94. A limit rod 96 is fixedly installed on the inner side of the N-shaped frame 93. The sliding block 95 slides and fits on the outer side of the limit rod 96. A three-jaw hydraulic chuck 97 is fixedly installed at the bottom of the sliding block 95, and a clamping claw 98 is slidably installed on the three-jaw hydraulic chuck 97. A silicone ring 99 is provided at the bottom of the clamping claw 98. In addition, six groups of N-shaped frames 93, linear modules 94, sliding blocks 95, limit rods 96 and three-jaw hydraulic chucks 97 are provided, and the six groups of N-shaped frames 93, linear modules 94, sliding blocks 95, limit rods 96 and three-jaw hydraulic chucks 97 are arranged in a circular array with equal spacing, with the center of the circle section of the circular frame 92 as the center of the array, and a single group of three-jaw hydraulic chucks 97 is provided. The clamping jaws 98 and the silicone ring 99 are provided with three groups, so as to better clamp and fix. The outer side of the silicone ring 99 is clamped with a conical cover 901, and the inner side of the conical cover 901 is threadedly installed with a reaction cylinder 902. The reaction cylinder 902 is filled with culture medium and cell body. The inner side of the top of the reaction cylinder 902 is clamped with a conical bucket 903, and the arc-shaped inner wall of the conical bucket 903 is fixedly installed with a handle 904. The outer side of the top of the conical bucket 903 is fixedly installed with a clamping block 905. The side wall of the top of the reaction cylinder 902 is provided with a clamping groove 906, and the clamping block 905 fits the reaction cylinder 902. On the inner side of the card slot 906, a dissolved oxygen sensor 907 is clamped inside the curved side wall of the reaction cylinder 902, and a top electric push rod 908 is fixedly installed at the bottom center of the three-jaw hydraulic chuck 97. A piston rod 909 is slidably installed inside the top electric push rod 908. The bottom of the piston rod 909 is fixedly connected to the top center of the sliding cylinder 9010. The curved side wall of the sliding cylinder 9010 is provided with a trapezoidal groove 9011. In addition, three groups of trapezoidal grooves 9011 are provided, and the top side length of the vertical cross-section of the trapezoidal groove 9011 is smaller than the bottom side length.

[0053] In one embodiment of the present invention, a mixing assembly 10 is provided on the reaction cylinder 902, and the mixing assembly 10 includes a bottom electric push rod 101, and the bottom of the reaction cylinder 902 is fixedly installed with the bottom electric push rod 101, and the outer side of the piston end of the bottom electric push rod 101 is provided with a sealing bearing 102 and a sealing rubber ring 103, and the sealing bearing 102 and the sealing rubber ring 103 are fixedly installed on the inner wall of the bottom end of the reaction cylinder 902, and the sealing bearing 102 is located below the sealing rubber ring 103, and the top of the piston end of the bottom electric push rod 101 is fixedly installed with a spring 104, and the inner side of the piston end of the bottom electric push rod 101 is fixedly installed with one end of a pressure sensor 105, and the other end of the pressure sensor 105 is attached to the bottom of the spring 104, so that the pressure sensor 105 can better obtain the pressure data received. In addition, a reduction motor 106 is fixedly installed at the bottom center of the reaction cylinder 902, and the reduction motor 106 outputs A rotating shaft 107 is fixedly installed at the output end, a center rod 108 is fixedly installed on the top of the rotating shaft 107, a sealed bearing 102 and a sealing rubber ring 103 are also arranged on the outside of the bottom end of the center rod 108, a circular ring 109 is fixedly installed on the outside of the center rod 108, and a stirring column 1010 is fixedly installed on the outside of the circular ring 109. In addition, six groups of reaction cylinders 902 are provided, and three groups of circular rings 109 are provided on the inner side of a single group of reaction cylinders 902, and the three groups of circular rings 109 are arranged in a linear array with equal spacing, four groups of stirring columns 1010 are provided on the single group of circular rings 109, and the outer side of the stirring column 1010 has an arc-shaped outer wall to avoid shear force generated by stirring and damage to the cell body. A fixing ring 1011 is fixedly installed at the bottom of the reaction cylinder 902, and the fixing ring 1011 is located on the outside of the bottom electric push rod 101 and the reduction motor 106, and cooperates with the fixing ring 1011 to better place the reaction cylinder 902 when not in use.

[0054] In one embodiment of the present invention, a rotating assembly 11 is provided on the inner side of the conical cover 901, and the rotating assembly 11 includes a support rod 111. The arc-shaped inner wall of the conical bucket 903 is fixedly installed with the support rod 111. Two groups of support rods 111 are provided, and an accelerator 112 is fixedly installed between the two groups of support rods 111. The top end of the center rod 108 is clamped on the bottom input end of the accelerator 112, and a rotating rod 113 is fixedly installed on the top output end of the accelerator 112, and a turbine 114 is fixedly installed on the outside of the rotating rod 113.

[0055] In one embodiment of the present invention, a sealing assembly 12 is provided on the outer side of the conical cover 901, and the sealing assembly 12 includes a top tube 121, and the top tube 121 is fixedly installed on the bottom of the sliding block 95, and the top tube 121 is located on the outside of the three-jaw hydraulic chuck 97. An arc ring 122 is fixedly installed on the bottom of the top tube 121, and a rubber ring 123 is fixedly installed on the inner side of the round cover 7. A coil spring 124 is provided inside the rubber ring 123. The outer side of the top tube 121 fits against the inner side of the rubber ring 123, thereby achieving a better sealing effect.

[0056] In one embodiment of the present invention, a moving assembly 13 is provided at the top of the machine body 1, and the moving assembly 13 includes a frame 131, and the frame 131 is fixedly installed on the other side of the top of the machine body 1, and a vertical hydraulic cylinder 132 is fixedly installed on the top of the frame 131, and a horizontal plate 133 is fixedly installed on the piston end of the vertical hydraulic cylinder 132, and a sliding sleeve 134 is fixedly installed inside the horizontal plate 133, and a sliding rod 135 is fixedly installed inside the frame 131, and the sliding sleeve 134 slides and fits on the outside of the sliding rod 135, and a horizontal hydraulic cylinder 136 is fixedly installed at the bottom of the horizontal plate 133, and a U-shaped frame 137 is fixedly installed on the piston end of the horizontal hydraulic cylinder 136 An asynchronous motor 138 is fixedly installed on the outside of the U-shaped frame 137, a threaded rod 139 is fixedly installed on the outside of the output shaft of the asynchronous motor 138, the threaded rod 139 is rotatably installed on the inside of the U-shaped frame 137, a threaded block 1310 is threadedly installed on the outside of the threaded rod 139, the bottom of the threaded block 1310 is fixedly connected to the top center of the servo motor 91, a slider 1311 is fixedly installed on the outside of the threaded block 1310, a slide rail 1312 is fixedly installed on the inside of the U-shaped frame 137, and the slider 1311 is slidably installed on the inside of the slide rail 1312, thereby improving the degree of automation of the device body and facilitating operation.

[0057] Among them, the electrical components appearing in this application document are all electrically connected to the controller 8 and the 220V AC power supply, and the controller 8 is a conventional known device that can control the heater 3, the solenoid valve 6, the servo motor 91, the linear module 94, the three-jaw hydraulic chuck 97, the dissolved oxygen sensor 907, the top electric push rod 908, the bottom electric push rod 101, the pressure sensor 105, the reduction motor 106, the vertical hydraulic cylinder 132, the horizontal hydraulic cylinder 136 and the asynchronous motor 138. The standard parts used in this application document can all be purchased from the market, and the specific connection methods of each part are connected by conventional means such as mature rivets and welding in the prior art, and the machinery, parts and equipment all adopt conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here.

[0058] The present invention also provides a method for using a reactor for cell culture, comprising the following steps:

[0059] S1. After inputting an appropriate amount of culture medium and cell bodies into the reaction cylinder 902, the operator holds the handle 904 and inserts the clamping block 905 into the clamping groove 906. With this operation, the conical bucket 903 can be tightly clamped into the inner side of the reaction cylinder 902. Then, the reaction cylinder 902 is screwed into the conical cover 901, and the top end of the conical cover 901 is sleeved on the outer side of the sliding cylinder 9010.

[0060] S2. When the controller 8 issues a start command, the servo motor 91 starts to operate, driving the round frame 92 to rotate slowly. At the same time, the linear module 94 on the N-shaped frame 93 is started to drive the linear module 94 to work, so that the sliding block 95 moves smoothly along the outer side of the limit rod 96, thereby prompting the three-jaw hydraulic chuck 97 to move. When the three-jaw hydraulic chuck 97 is started, multiple groups of clamping jaws 98 and soft silicone rings 99 cooperate with each other to clamp and fix the conical cover 901, ensuring that it remains stable in subsequent operations;

[0061] S3, the controller 8 issues a command to start the vertical hydraulic cylinder 132 on the frame 131, and cooperates with the mechanical structure of the horizontal plate 133, the sliding sleeve 134 and the sliding rod 135 to enable the horizontal hydraulic cylinder 136 to move up and down flexibly. Then the horizontal hydraulic cylinder 136 is started, and the lateral thrust generated by it makes the U-shaped frame 137 move left and right, and then the asynchronous motor 138 is started to drive the threaded rod 139 to rotate at high speed, and cooperates with the transmission combination of the threaded block 1310, the slider 1311 and the slide rail 1312 to enable the servo motor 91 to achieve multi-axis motion to meet different operation requirements;

[0062] S4, accurately align the top cylinder 121 with the rubber ring 123, apply a certain external force, so that the arc ring 122 moves slowly downward, gradually squeezing the rubber ring 123, the rubber ring 123 is elastically deformed by the force, and the coil spring 124 also shrinks and deforms accordingly. Through such structural design and operation, the sealing effect is enhanced. At this time, the reaction cylinder 902 as a whole smoothly enters the interior of the culture barrel 2;

[0063] S5, cooperate with the heater 3, generate heat through the internal heating element, and accurately control the temperature of the inside of the culture barrel 2 to ensure that the temperature of the culture environment is suitable for cell growth. At the same time, cooperate with the air inlet pipe 4, the air outlet pipe 5 and the solenoid valve 6. When the solenoid valve 6 is opened, the air inlet pipe 4 can smoothly input the external oxygen into the inside of the culture barrel 2 to meet the cell respiration needs, and when the waste gas is generated, the waste gas is discharged through the air outlet pipe 5 to maintain the gas balance of the culture environment;

[0064] S6. Cooperate with the dissolved oxygen sensor 907, whose probe is extended into the reaction tube 902 to obtain the oxygen content therein. Specifically, the dissolved oxygen sensor 907 can monitor the dissolved oxygen concentration in the culture system in real time, and transmit this signal to the controller 8 in time. The controller 8 sets the optimal dissolved oxygen range in advance according to the cell growth characteristics. When the dissolved oxygen sensor 907 detects that the dissolved oxygen concentration is lower than the set lower limit, the controller 8 will quickly issue an instruction to start the top electric push rod 908. The top electric push rod 908 makes the piston rod 909 drive the sliding tube 9010 and the trapezoidal groove 9011 to move upward, so that the trapezoidal groove 9011 exposes more area of ​​the conical cover 901, thereby increasing the amount of oxygen entering the reaction tube 902; when the dissolved oxygen concentration is higher than the set upper limit, the controller 8 will issue an instruction to reduce the oxygen supply. Through such a dynamic adjustment mechanism, the dissolved oxygen concentration is maintained within a suitable range to ensure the normal growth of cells;

[0065] S7, start the bottom electric push rod 101, cooperate with the sealing bearing 102 and the sealing rubber ring 103, so that the bottom electric push rod 101 drives the spring 104 and the pressure sensor 105 to move up and down smoothly, and the pressure sensor 105 can accurately convert the acquired pressure value into the viscosity data of the mixture, and the data is fed back to the controller 8, and the controller 8 starts the reduction motor 106 according to the preset program servo, and the output shaft of the reduction motor 106 drives the rotating shaft 107 to rotate, thereby rotating the center rod 108, and the rotation of the center rod 108 cooperates with the ring 109 to make the stirring column 1010 rotate at a slower speed, so as to fully stir the mixture and make the cell body fully contact with the culture medium, thereby improving the culture effect of the cell body, and the whole operation process is more convenient;

[0066] S8. When the central rod 108 rotates, the power generated is transmitted to the accelerator 112 on the support rod 111. The accelerator 112 (the accelerator 112 is an existing device and is not described in detail here) enhances the power, so that the rotating rod 113 rotates faster. The high-speed rotation of the rotating rod 113 drives the turbine 114 connected thereto to rotate synchronously. The rotation of the turbine 114 can better transport oxygen to the interior of the reaction tube 902, provide sufficient oxygen for the breathing and growth of cells, and ensure the smooth progress of the cell culture process.

[0067] The above generally describes the present invention in detail, but it is obvious to a person skilled in the art that some modifications or improvements can be made to the present invention. Therefore, modifications or improvements that do not depart from the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. A reactor for cell culture, comprising a body (1), a culture barrel (2) fixedly mounted on one side of the top of the body (1), a heater (3) arranged on the outer side of the bottom end of the culture barrel (2), an air inlet pipe (4) and an air outlet pipe (5) fixedly mounted on both sides of the top of the culture barrel (2), solenoid valves (6) arranged on the air inlet pipe (4) and the air outlet pipe (5), an oxygen supply device connected to the outer side of the air inlet pipe (4), an exhaust gas collection device connected to the outer side of the air outlet pipe (5), a round cover (7) fixedly mounted on the top of the culture barrel (2), and a controller (8) fixedly mounted on the top of the body (1), characterized in that: A culture component (9) is disposed at the top of the body (1), and the culture component (9) comprises: A servo motor (91), the body (1) is provided with a servo motor (91) on the outside, the output shaft of the bottom end of the servo motor (91) is fixedly mounted on the outside of the center of the round frame (92), the bottom of the round frame (92) is fixedly mounted with an N-shaped frame (93), the top inner side of the N-shaped frame (93) is fixedly mounted with a linear module (94), the inner side of the linear module (94) is slidably mounted with a sliding block (95), the inner side of the N-shaped frame (93) is fixedly mounted with a limit rod (96), the sliding block (95) is slidably fitted on the outer side of the limit rod (96), the bottom of the sliding block (95) is fixedly mounted with a three-jaw hydraulic chuck (97), the three-jaw hydraulic chuck (97) is slidably mounted with a clamping jaw (98), and the bottom end of the clamping jaw (98) is sleeved with a silicone ring (99); A conical cover (901), the outer side of the silicone ring (99) is clamped with the conical cover (901), the inner side of the conical cover (901) is threadedly mounted with a reaction cylinder (902), the reaction cylinder (902) is filled with culture medium and cell bodies, the inner side of the top of the reaction cylinder (902) is clamped with a conical bucket (903), the arc-shaped inner wall of the conical bucket (903) is fixedly mounted with a handle (904), the outer side of the top of the conical bucket (903) is fixedly mounted with a clamping block (905), the top side wall of the reaction cylinder (902) is provided with a clamping groove (906), the clamping block (905) is attached to the inner side of the clamping groove (906), and the inner side of the arc-shaped side wall of the reaction cylinder (902) is clamped with a dissolved oxygen sensor (907); A top electric push rod (908) is fixedly installed at the bottom center of the three-jaw hydraulic chuck (97), a piston rod (909) is slidably installed inside the top electric push rod (908), the bottom of the piston rod (909) is fixedly connected to the top center of the sliding cylinder (9010), and the arc-shaped side wall of the sliding cylinder (9010) is provided with a trapezoidal groove (9011).

2. The reactor for cell culture according to claim 1, characterized in that: The N-shaped frame (93), the linear module (94), the sliding block (95), the limiting rod (96) and the three-jaw hydraulic chuck (97) are provided in multiple groups, and the multiple groups of the N-shaped frame (93), the linear module (94), the sliding block (95), the limiting rod (96) and the three-jaw hydraulic chuck (97) are all arranged in a circular array with equal spacing, with the center of the circle cross section of the circular frame (92) as the center of the array, and the clamping jaws (98) and the silicone ring (99) on a single group of the three-jaw hydraulic chuck (97) are provided in three groups.

3. The reactor for cell culture according to claim 2, characterized in that: The trapezoidal grooves (9011) are provided in multiple groups, and the top side length of the vertical cross section of the trapezoidal grooves (9011) is smaller than the bottom side length.

4. The reactor for cell culture according to claim 3, characterized in that: The reaction cylinder (902) is provided with a mixing assembly (10), and the mixing assembly (10) comprises a bottom electric push rod (101), the bottom of the reaction cylinder (902) is fixedly mounted with the bottom electric push rod (101), the outer side of the piston end of the bottom electric push rod (101) is provided with a sealing bearing (102) and a sealing rubber ring (103), and the sealing bearing (102) and the sealing rubber ring (103) are both fixedly mounted on the inner wall of the bottom end of the reaction cylinder (902), the sealing bearing (102) is located below the sealing rubber ring (103), the top of the piston end of the bottom electric push rod (101) is fixedly mounted with a spring piece (104), the inner side of the piston end of the bottom electric push rod (101) is fixedly mounted with one end of a pressure sensor (105), and the other end of the pressure sensor (105) is attached to the bottom of the spring piece (104).

5. The reactor for cell culture according to claim 4, characterized in that: A reduction motor (106) is fixedly installed at the center of the bottom of the reaction cylinder (902), a rotating shaft (107) is fixedly installed at the output end of the reduction motor (106), a center rod (108) is fixedly installed at the top of the rotating shaft (107), a sealing bearing (102) and a sealing rubber ring (103) are also arranged on the outer side of the bottom end of the center rod (108), a circular ring (109) is fixedly installed on the outer side of the central rod (108), a stirring column (1010) is fixedly installed on the outer side of the circular ring (109), and a fixing ring (1011) is fixedly installed at the bottom of the reaction cylinder (902), and the fixing ring (1011) is located on the outer side of the bottom electric push rod (101) and the reduction motor (106).

6. The reactor for cell culture according to claim 5, characterized in that: The reaction cylinders (902) are provided in multiple groups, and the circular rings (109) on the inner side of a single group of reaction cylinders (902) are provided in multiple groups, and the multiple groups of circular rings (109) are arranged in a linear array with equal spacing, and the stirring columns (1010) on the single group of circular rings (109) are provided in multiple groups, and the outer side of the stirring columns (1010) is an arc-shaped outer wall.

7. The reactor for cell culture according to claim 6, characterized in that: A rotating assembly (11) is arranged inside the conical cover (901), and the rotating assembly (11) comprises a support rod (111). The arc-shaped inner wall of the conical bucket (903) is fixedly mounted with the support rod (111). The support rod (111) is provided in multiple groups, and an accelerator (112) is fixedly mounted between the multiple groups of the support rods (111). The top end of the central rod (108) is clamped to the bottom input end of the accelerator (112), and the top output end of the accelerator (112) is fixedly mounted with a rotating rod (113), and the outer side of the rotating rod (113) is fixedly mounted with a turbine (114).

8. The reactor for cell culture according to claim 7, characterized in that: A sealing assembly (12) is arranged on the outside of the conical cover (901), and the sealing assembly (12) includes a top tube (121). The bottom of the sliding block (95) is fixedly mounted with the top tube (121), and the top tube (121) is located on the outside of the three-jaw hydraulic chuck (97). An arc ring (122) is fixedly mounted on the bottom of the top tube (121). A rubber ring (123) is fixedly mounted on the inside of the round cover (7), and a coil spring (124) is arranged inside the rubber ring (123). The outside of the top tube (121) is in contact with the inside of the rubber ring (123).

9. The reactor for cell culture according to claim 8, characterized in that: A moving assembly (13) is arranged at the top of the machine body (1), and the moving assembly (13) comprises a frame (131). The frame (131) is fixedly mounted on the other side of the top of the machine body (1). A vertical hydraulic cylinder (132) is fixedly mounted on the top of the frame (131). A horizontal plate (133) is fixedly mounted on the piston end of the vertical hydraulic cylinder (132). A sliding sleeve (134) is fixedly mounted inside the horizontal plate (133). A sliding rod (135) is fixedly mounted inside the frame (131). The sliding sleeve (134) is slidably fitted on the outside of the sliding rod (135). A horizontal hydraulic cylinder (136) is fixedly mounted on the bottom of the horizontal plate (133). The piston end of the horizontal hydraulic cylinder (136) is fixedly mounted. A U-shaped frame (137) is installed, an asynchronous motor (138) is fixedly installed on the outside of the U-shaped frame (137), a threaded rod (139) is fixedly installed on the outside of the output shaft of the asynchronous motor (138), the threaded rod (139) is rotatably installed on the inside of the U-shaped frame (137), a threaded block (1310) is threadedly installed on the outside of the threaded rod (139), the bottom of the threaded block (1310) is fixedly connected to the top center of the servo motor (91), a slider (1311) is fixedly installed on the outside of the threaded block (1310), a slide rail (1312) is fixedly installed on the inside of the U-shaped frame (137), and the slider (1311) is slidably installed on the inside of the slide rail (1312).

10. A method for using the reactor for cell culture according to claim 9, characterized in that: The following steps are involved: S1, after the culture medium and the cell bodies are input into the reaction cylinder (902), the conical bucket (903) is clamped into the inner side of the reaction cylinder (902) by means of the handle (904), the clamping block (905) and the clamping groove (906), and then the reaction cylinder (902) is screwed into the conical cover (901), and the top end of the central rod (108) is clamped to the bottom end of the accelerator (112), and the top end of the conical cover (901) is sleeved on the outer side of the sliding cylinder (9010); S2, when the servo motor (91) is started by the controller (8), the round frame (92) is rotated, and the three-jaw hydraulic chuck (97) is moved in cooperation with the N-shaped frame (93), the linear module (94), the sliding block (95) and the limit rod (96). When the three-jaw hydraulic chuck (97) is started, the conical cover (901) can be clamped and fixed in cooperation with the clamping claws (98) and the silicone ring (99); S3, the vertical hydraulic cylinder (132) on the frame (131) is started by the controller (8), and the horizontal hydraulic cylinder (136) is moved up and down in cooperation with the horizontal plate (133), the sliding sleeve (134) and the sliding rod (135), and the threaded rod (139) is rotated in cooperation with the U-shaped frame (137) and the asynchronous motor (138), and the servo motor (91) is moved in multiple axes in cooperation with the threaded block (1310), the sliding block (1311) and the sliding rail (1312); S4, aligning the top tube (121) with the rubber ring (123), so that the arc ring (122) moves downward to squeeze the rubber ring (123), and at the same time the coil spring (124) contracts and deforms, so that the sealing effect is better. At this time, the reaction tube (902) as a whole enters the interior of the culture barrel (2); S5, in conjunction with the heater (3), the air inlet pipe (4), the air outlet pipe (5) and the solenoid valve (6), can control the temperature inside the culture barrel (2), input oxygen and discharge waste gas; S6, cooperate with the dissolved oxygen sensor (907) to obtain the oxygen content inside the reaction cylinder (902), thereby transmitting a signal to the controller (8), starting the top electric push rod (908), so that the piston rod (909) drives the sliding cylinder (9010) and the trapezoidal groove (9011) to move up and down, thereby changing the amount of oxygen entering the reaction cylinder (902); S7, starting the bottom electric push rod (101), cooperating with the sealing bearing (102) and the sealing rubber ring (103), so that the bottom electric push rod (101) drives the spring piece (104) and the pressure sensor (105) to move up and down, thereby cooperating with the pressure sensor (105) to convert the acquired pressure value into the viscosity of the mixture, thereby cooperating with the controller (8) to servo start the reduction motor (106), cooperating with the rotating shaft (107), the center rod (108), the ring (109) and the stirring column (1010), and stirring the mixture; S8. When the central rod (108) rotates, the supporting rod (111), the accelerator (112) and the rotating rod (113) are coordinated to make the turbine (114) rotate, thereby better transporting oxygen to the interior of the reaction tube (902).

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