Method for promoting germination of Brachionium molatum seeds

Abstract

The invention provides a method for promoting germination of Morlat hybrid Brachiform grass seeds, and belongs to the technical field of Brachiform grass seed germination, and the specific technical scheme is as follows: S1, pretreating the seeds for later use; s2, the seeds pretreated in the S1 are put into 30% hydrogen peroxide to be soaked for 25 min to 30 min, after treatment, distilled water is used for cleaning, and hydrogen peroxide attached to the seeds is removed; s3, the seeds treated in the S2 are placed in a culture dish and germinated under the conditions that the temperature of an artificial climate box is set to be (25 + / -1) DEG C, illumination is conducted for 12 h, darkness is conducted for 12 h, and the germination time is 14 d. According to the method, the germination rate of the Brachionium bracteatum-Morlaite II seeds can be obviously increased, and the treatment effect reaches a remarkable level. The research result provides technical support for improving the germination rate of Morlat hybrid Brachionium seeds and ensuring the seeding quality.

Description

Technical Field

[0001] The invention relates to the technical field of Brachiaria molata seed germination, and more particularly to a method for promoting Brachiaria molata seed germination. Background Art

[0002] Brachiaria decumbens Stapf is a perennial herb belonging to the genus Brachiaria of the Poaceae family. This variety has the excellent characteristics of vigorous plant growth, few diseases and pests, heat resistance, drought resistance, good palatability, high quality, high dry matter yield, strong adaptability, can be planted in acidic clay, and can maintain good growth under adverse environmental conditions. It can provide excellent forage all year round, is suitable for mowing and grazing, and can also be used for slope protection and soil and water conservation. However, the seed dormancy phenomenon of Morat hybrid Brachiaria is serious, and the germination rate is very low, which affects its application in production. The seed germination rate depends not only on the vitality of the seeds, but also on whether the seeds are in a dormant state. Seed dormancy refers to the phenomenon that viable seeds cannot germinate under suitable germination conditions. It is a biological adaptation that plants have acquired in the long-term evolution process in order to adapt to the environment. It is extremely beneficial to the preservation and reproduction of "species" (Chen Wei et al., 2009, Yan Qichuan, 2001). The degree of dormancy reflects the external conditions required for seed germination. The width of the environment required for germination (including temperature, moisture, light, etc.) can reflect the depth of seed dormancy. Seed germination experiments are very important for agricultural production and the study of plant production performance. In order to ensure the maximum benefit of forage production and maximize the germination rate of forage seeds, it is more effective to use effective methods to artificially break seed dormancy. Morat hybrid Brachiaria has various excellent characteristics. Improving the germination rate of this variety of seeds is a support and guarantee for increasing forage yield and benefits. Therefore, screening out the best treatment method for breaking the dormancy of Morat hybrid Brachiaria seeds and improving the germination rate of Brachiaria seeds has important practical guiding significance for grass production. Summary of the invention

[0003] The invention aims to provide a method for promoting the germination of Brachiaria molatii hybrid seeds, breaking the dormancy of Brachiaria molatii hybrid seeds and promoting the germination of Brachiaria molatii hybrid seeds.

[0004] The above technical purpose of the present invention is achieved through the following technical scheme: a method for promoting the germination of Morat hybrid Brachiaria seeds, comprising the following steps:

[0005] S1. Pre-treat the seeds for later use;

[0006] S2. Soak the seeds pretreated in S1 in 30% hydrogen peroxide for 25-30 minutes, and then wash with distilled water to remove the hydrogen peroxide on the seeds;

[0007] S3. Place the seeds treated with S2 on the seedbed and germinate them at 24℃-26℃, 12h light and 12h dark. The germination time is 14d. During the observation process, add an equal amount of distilled water every day to maintain the humidity of the seedbed.

[0008] The present invention is further configured as follows: in step S1, the pretreatment is to rinse with clean water to remove shrunken seeds, and then use filter paper to absorb moisture on the surface of the seeds.

[0009] The present invention is further configured as follows: the soaking time of the seeds in the 30% hydrogen peroxide solution is 25 minutes to 30 minutes.

[0010] The present invention is further configured as follows: in step S3, the seed bed is a culture dish covered with filter paper, distilled water is added to slightly cover the filter paper to moisten it, or it is a culture tray filled with sterilized nutrient soil.

[0011] The present invention is further configured as follows: the sterilized nutrient soil is natural soil: humus soil: vermiculite = 2:2:1.

[0012] In summary, the present invention has the following beneficial effects:

[0013] The method provided by the present invention, 30% hydrogen peroxide can change the permeability of seeds, directly or indirectly change the inhibitory substances in the seed shell, break the seed dormancy, and improve the germination rate of seeds. Soaking seeds with 30% hydrogen peroxide for 25min-30min can significantly improve the germination rate of Brachiaria-Morat II seeds, and the treatment effect reaches a significant level. The research results provide technical support for improving the germination rate of Brachiaria-Morat hybrid seeds and ensuring the sowing quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a bar graph showing the effect of various treatment methods on the germination rate of Brachiaria molatii seeds (where different letters between the germination rates of seeds treated by different methods indicate significant differences between the germination rates (P < 0.05));

[0015] Figure 2 It is a bar graph showing the effects of various treatment methods on the germination potential of Brachiaria molatii hybrid seeds (different letters between the germination rates of seeds treated with different methods indicate significant differences in germination rates (P < 0.05));

[0016] Figure 3It is a bar graph showing the effect of each treatment method on the germination index of Brachiaria molata seeds (where different letters between the germination rates of seeds treated by different methods indicate significant differences between the germination rates (P < 0.05));

[0017] Figure 4 The effect of different treatment time of 30% hydrogen peroxide on the germination potential of Brachiaria seeds (the different letters between the germination rates of seeds treated by different methods indicate significant differences (P<0.05));

[0018] Figure 5 The figure shows the effect of different treatment times of 30% hydrogen peroxide on the germination rate of Brachiaria seeds (the different letters between the germination rates of seeds treated by different methods indicate significant differences (P<0.05)). DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Test Materials:

[0021] The experimental material was Brachiaria decumbens Stapf. from Ubon Ratchathani, Thailand.

[0022] Seed germination test

[0023] With reference to the local standards of Yunnan Province (see: Yunnan Provincial Technical Supervision Bureau. DB / 5300B20001-91, Forage Seed Inspection Procedure [S]. 1991.), the germination method on culture dish paper was adopted, and 4 different treatment groups were set up: Treatment Group 1: 30% hydrogen peroxide method (30% hydrogen peroxide soaking for 30 minutes); Treatment Group 2: concentrated sulfuric acid method (98% concentrated sulfuric acid soaking for 15 minutes); Treatment Group 3: concentrated sulfuric acid and potassium nitrate method (98% concentrated sulfuric acid soaking for 15 minutes, then washing the seeds with distilled water, and then adding 100g / L potassium nitrate to soak the seeds for 6 hours); Treatment Group 4: warm water soaking method (70℃ warm water soaking for 2 minutes), with distilled water as the control (CK: distilled water soaking for 30 minutes), seed germination test was carried out.

[0024] Example 1

[0025] S1: Seed pretreatment: randomly weigh a certain number of seeds, rinse with clean water to remove the shrunken seeds, and then use filter paper to absorb the moisture on the surface of the seeds, and wait for the germination test;

[0026] S2: 30% hydrogen peroxide method, rinse the seeds with clean water, select 50 sample seeds and put them into a beaker, then add an appropriate amount of 30% hydrogen peroxide to soak the seeds for 30 minutes, and after treatment, use distilled water to wash away the hydrogen peroxide on the seeds.

[0027] S3: Place a layer of filter paper on the bottom of each washed and dried culture dish, and moisten the filter paper with an equal amount of distilled water. Count 50 seeds treated according to S2 and place them in each culture dish at a certain interval. Place the culture dish in an artificial climate box for seedling cultivation, with 3 biological replicates. The germination period is 14 days, and the germination temperature is controlled at (25±1)℃, 12h light; (25±1)℃, 12h dark. Add an equal amount of distilled water every day to maintain the humidity in the culture dish. Observe and record the germination after various treatments, and count the germination potential on the 12th day and the germination rate on the 14th day.

[0028] Comparative Example 1

[0029] The difference from Example 1 is that, in step S2, the concentrated sulfuric acid method is used. 50 sample seeds are selected and placed in a beaker for rinsing with clean water. The seeds are placed in the beaker and soaked in 98% concentrated sulfuric acid for 15 minutes. Then, they are rinsed with distilled water and placed in a culture dish. The biological process is repeated 3 times.

[0030] Comparative Example 2

[0031] The difference from Example 1 is that step S2 uses concentrated sulfuric acid and potassium nitrate treatment. 50 sample seeds were rinsed and screened with clean water, and the seeds were placed in a beaker and soaked in 98% concentrated sulfuric acid for 15 minutes. The seeds were then washed with distilled water, and the washed seeds were placed in a beaker and 100 g / L potassium nitrate was added to completely immerse the seeds in the solution. The seeds were taken out after soaking for 6 hours. The seeds were then washed with distilled water, and the treated seeds were placed in a culture dish, with 3 biological replicates.

[0032] Comparative Example 3

[0033] The difference from Example 1 is that step S2 uses the warm water seed soaking method. 50 sample seeds after rinsing are selected and placed in a beaker filled with 70°C warm water, so that the seeds are completely immersed in the warm water, and then the beaker is covered with a glass sheet. After 2 minutes, the seeds are taken out and placed in a culture dish, and the biological repetition is repeated 3 times.

[0034] Comparative Example 4

[0035] The difference from Example 1 is that, in step S2, the rinsed seeds are soaked in distilled water for 30 minutes, with 3 biological replicates.

[0036] Measurement items and methods

[0037] Seeds that have sprouted are classified as germinated when the sprout grows to half the length of the seed. Seeds that have incomplete, deformed or rotten sprouts or shrunken roots are not considered germinated.

[0038] Observe and record the germination status after various treatments, count the germination potential on the 12th day, and count the germination rate on the 14th day.

[0039] Calculate the germination potential, germination index and germination rate according to the following formula.

[0040] Germination rate (%) = number of germinated seeds at the end of germination period (14 days) ÷ number of test seeds × 100%

[0041] Germination potential (%) = number of seeds that germinate normally at the initial germination stage (12 days) ÷ number of seeds tested × 100%

[0042] Germination index (GI) = Σ(Gt / Dt), where: Gt refers to the number of seeds germinated t days after soaking; Dt refers to the corresponding germination days.

[0043] Data processing

[0044] The germination rate, germination potential and germination index of different treatments were measured or calculated. Data collation and drawing were completed using Excel 2019, and statistical analysis was performed using SPSS 22.0 software.

[0045] The following are the test results and analysis:

[0046] (1) Effects of various treatments on the germination rate of Brachiaria molata seeds

[0047] Effects of various treatment methods on the germination rate of Brachiaria molata hybrid seeds (Attachment Figure 1 ) showed that the germination rate of Brachiaria molat II hybrid seeds after various treatments ranged from 6.00% to 34.67%. Among them, the germination rate of Brachiaria molat II hybrid seeds treated with 30% hydrogen peroxide was significantly higher than that of other treatments and the control (P﹤0.05). The germination rate of Brachiaria molat II hybrid seeds treated with concentrated sulfuric acid was significantly higher than that of potassium nitrate and concentrated sulfuric acid (P﹤0.05).

[0048] (2) Effects of various treatment methods on the germination potential of Brachiaria molata hybrid seeds

[0049] By the attached Figure 2 The results showed that the germination potential of Brachiaria molatii hybrid seeds varied from 4.00% to 31.33% after various treatments. The germination potential of Brachiaria molatii hybrid seeds treated with 30% hydrogen peroxide was significantly higher than that of other treatments and the control (P﹤0.05). The germination potential of Brachiaria molatii hybrid seeds treated with concentrated sulfuric acid was significantly higher than that of potassium nitrate and concentrated sulfuric acid, distilled water and warm water (P﹤0.05).

[0050] (3) Effects of various treatment methods on the germination index of Brachiaria molata seeds

[0051] From the attached Figure 3 It can be seen that the germination index of Brachiaria molatii seeds after various treatments ranged from 0.62 to 7.47. Among them, the germination index of Brachiaria molatii seeds treated with 30% hydrogen peroxide was significantly higher than that of other treatments (P﹤0.05).

[0052] Here are the conclusions of the test:

[0053] Germination rate is an important indicator to measure the quality of seeds, which reflects the amount of seed germination; germination potential is one of the main indicators to reflect the quality of seeds, reflecting the speed and uniformity of seed germination; germination index reflects the comprehensive vitality of seeds during the entire germination period. Studies have shown that 30% hydrogen peroxide can change the permeability of seeds, directly or indirectly change the inhibitory substances in the seed shell, break the seed dormancy, and improve the germination rate of seeds. Treating seeds with 30% hydrogen peroxide can significantly improve the germination rate of Brachiaria-Morat II seeds, and the treatment effect reaches a significant level. Acid treatment can soften the hard seed coat and easily penetrate water and air. Since strong acid can corrode the seed coat and change the permeability of the seed coat, thereby promoting seed germination, using strong acid and strong alkali to treat forage seeds can improve the germination rate of seeds. In this experiment, concentrated sulfuric acid was used to treat seeds, which increased the germination rate of Morat II hybrid Brachiaria compared with the distilled water control, but the difference was not significant, but the germination potential was significantly improved. Concentrated sulfuric acid and potassium nitrate treatment is the most widely used chemical method to promote seed germination. There are many studies on using warm water to treat seeds to improve seed germination rate. This experiment used 70℃ warm water to treat seeds, but the germination rate of Morat II hybrid Brachiaria seeds was only slightly higher than that of the control group, but the difference was not significant.

[0054] In summary, among the several treatment methods used, 30% hydrogen peroxide treatment of Morat II hybrid Brachiaria seeds had the best effect, and the germination rate, germination potential and germination index were significantly higher than those of other treatments (P﹤0.05).

[0055] Example 2

[0056] The difference from Example 1 is that in step S2, the soaking time of 30% hydrogen peroxide is 20min, 25min, 30min, 35min, and 40min, respectively, while the soaking time of distilled water for 30min is used as a control.

[0057] Experimental results and analysis:

[0058] like Figure 4 As shown:

[0059] When Morat II hybrid Brachiaria seeds were treated with 30% hydrogen peroxide, the germination potential varied in the range of 24.33% to 32.00% at different treatment times. Among them, the germination potential of seeds treated with 30% hydrogen peroxide for 25 minutes and 30 minutes was significantly higher than that of seeds treated for 20 minutes and the control (P﹤0.05). The germination potential of seeds treated with 30% hydrogen peroxide for all treatment times was significantly higher than that of the control (germination potential was 4.33%) (P﹤0.05).

[0060] like Figure 5 As shown:

[0061] When Morat II hybrid Brachiaria seeds were treated with 30% hydrogen peroxide, the germination rate varied from 28.67% to 34.67% at different treatment times. The germination rate of seeds treated with 30% hydrogen peroxide for 25 minutes and 30 minutes was significantly higher than that of seeds treated for 20 minutes and the control (P﹤0.05). The germination rate of seeds treated with 30% hydrogen peroxide for all treatment times was significantly higher than that of the control (germination rate was 5.67%) (P﹤0.05).

[0062] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for promoting the germination of Brachiaria molata hybrid seeds, characterized in that: The steps include: S1. Pre-treat the seeds for later use; S2. Soak the seeds pretreated in S1 in 30% hydrogen peroxide for 25-30 minutes, and then wash with distilled water to remove the hydrogen peroxide on the seeds; S3. Place the seeds treated with S2 on the seedbed and germinate at 24℃-26℃, 12h light and 12h dark. The germination time is 14d. During the observation process, add an equal amount of distilled water every day to maintain the humidity of the seedbed.

2. A method for promoting the germination of Brachiaria molata seeds according to claim 1, characterized in that In step S1, the pretreatment is to rinse with distilled water to remove the shrunken seeds, and then use filter paper to absorb the moisture on the surface of the seeds.

3. A method for promoting the germination of Brachiaria molatii seeds according to claim 1, characterized in that: The soaking time of the seeds in 30% hydrogen peroxide is 25min-30min.

4. A method for promoting the germination of Brachiaria molata hybrid seeds according to claim 1, characterized in that: In step S3, the seed bed is a culture dish covered with filter paper, and sterilized distilled water is added to slightly cover the filter paper to moisten it, or it is a culture tray filled with sterilized nutrient soil.

5. A method for promoting germination of Brachiaria molata seeds according to claim 4, characterized in that: The nutrient soil is natural soil: humus soil: vermiculite = 2:2:1.

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