Chicory tea and production method thereof

Abstract

The invention belongs to the technical field of substitute tea quality and plant physiology, and particularly relates to chicory tea and a production method thereof. The method comprises the following steps: applying a chicory growth composition to soil where chicory grows, putting earthworm seedlings into the soil, and harvesting chicory leaves to prepare chicory tea after the chicory leaves grow out, the chicory growth composition is prepared from the following raw materials in parts by weight: 1-50 parts of nitrogen-fixing bacteria and 1-100 parts of a natural plant fiber material with water absorption performance. According to the method provided by the invention, the content of the chicoric acid is effectively increased, and a brand new perspective is provided for improving the quality of the chicoric tea substitute and increasing the content of the chicoric acid.

Description

Technical Field

[0001] The invention belongs to the technical fields of environmentally friendly materials, soil improvement and functional tea, and particularly relates to chicory tea and a production method thereof. Background Art

[0002] Chicory (Cichorium intybus L.) is a perennial herbaceous plant of the genus Cichorium in the Asteraceae family. It has strong adaptability and can grow in a variety of climatic conditions and ecological environments. It has important value in ecological restoration, soil and water conservation, etc. It has high nutritional value and is rich in crude protein, crude fiber, and crude fat. It is a good animal feed. At the same time, chicory is of great significance in the medical field. It has the effects of clearing away heat and detoxification, promoting bile secretion, diuresis, reducing swelling, and strengthening the stomach and digestion. It is also helpful to improve the body's resistance. Preliminary studies have shown that chicory granules can treat hyperuricemia. The course of treatment is 1 month, and the total clinical effective rate is 83.33%, and the average reduction of blood uric acid is 23.41%. It also has the effects of regulating blood lipids and protecting the liver. However, its clinical efficacy and safety are still unclear, and clinical studies are urgently needed to evaluate it. The mechanism of action needs to be further explored.

[0003] Chicoric acid is a type of caffeic acid derivative mainly isolated from plants of the Asteraceae family. It is an important active ingredient in chicory. Its relationship with the improvement of the quality of chicory tea is as follows: (1) Enhanced health care function: Chicoric acid is an important active ingredient in chicory plants and has a variety of pharmacological activities, such as improving the body's immunity, anti-tumor, anti-viral, antioxidant, lowering blood sugar and cholesterol. These effects make chicory tea rich in chicoric acid show significant advantages in promoting human health. (2) Promote the absorption of nutrients: Chicoric acid, as an organic acid, helps promote the absorption and utilization of other nutrients in tea, such as minerals and vitamins. This is of great significance for improving the overall nutritional value of chicory tea. (3) Increase the stability and shelf life of tea: Chicoric acid has a certain antioxidant capacity, which can help slow down the oxidation process of other ingredients in tea, thereby maintaining the freshness and quality of tea. In addition, it also has a certain inhibitory effect on the growth of microorganisms, which helps to extend the shelf life of chicory tea.

[0004] At present, increasing the content of chicoric acid in chicory tea is a difficult point in the production process of chicory tea. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a chicory tea and a production method thereof, so as to increase the chicoric acid content.

[0006] The present invention is achieved through the following technical solutions:

[0007] A method for producing chicory tea comprises applying a chicory growth composition to soil where chicory grows, placing earthworm seedlings in the soil, and harvesting chicory leaves when they grow to make chicory tea; the application amount of the chicory growth composition is 79.62 g / m 2 ~477.71g / m 2 The amount of earthworm seedlings released is 1 / m 2 ~10 pieces / m 2 .

[0008] The chicory growth composition is prepared from the following raw materials by weight: 1 to 50 parts of nitrogen-fixing bacteria and 1 to 100 parts of natural plant fiber materials with water absorption performance.

[0009] Preferably, the chicory growth composition is made of the following raw materials by weight: 1 to 15 parts of nitrogen-fixing bacteria and 5 to 60 parts of natural plant fiber materials with water absorption properties.

[0010] Preferably, the earthworm seedlings are at least one of Pheretima aspergillum, Pheretima carnosa and Shanghai Pheretima.

[0011] Preferably, the natural plant fiber material with water absorption performance is a water-absorbing bamboo fiber material.

[0012] Preferably, the method for preparing the water-absorbing bamboo fiber material comprises the following steps:

[0013] Use bamboo stems of 250-350 days old as materials, wash them, and grind them into bamboo powder of 75-85 meshes.

[0014] use 60 Co irradiation source, irradiation dosage 15kGy-20kGy, temperature 60℃-65℃, irradiation treatment 5min-6min, to change the primary molecular structure of bamboo fiber, to obtain the water-absorbing bamboo fiber material.

[0015] Preferably, the nitrogen-fixing bacteria is rhizobium powder, and the concentration of live bacteria in the powder is 100 million CFU / g to 200 million CFU / g.

[0016] Preferably, the soil should maintain the following conditions before earthworm seedlings are placed: the soil moisture content is maintained at 40% to 50%; the soil pH value is 6 to 8; and the soil temperature is maintained at 20°C to 25°C.

[0017] Preferably, before the earthworm seedlings are placed in the soil, a density of 1 earthworm / m 2 ~2 pieces / m 2A trench with a depth of 8 cm to 12 cm is opened, and then earthworm seedlings are placed in the trench; the number of earthworm seedlings placed in each trench is 1 to 10.

[0018] Preferably, the production process of harvesting chicory leaves to make chicory tea comprises the following steps:

[0019] Spread out the fresh chicory leaves, the temperature is 15℃~26℃, the humidity is 65%~85%, grab the leaves and they are soft. When the moisture content is 55%~60%, the leaves are ready to be dried.

[0020] Entering the rolling stage, the temperature is 20℃~26℃, the humidity is 85%~90%, and the rolling is carried out for 40min~55min, and the strips are tightly tied; after being taken out, ferment at a fermentation temperature of 25℃~27℃ and a humidity of 90%~100% for 2h~2.5h, and then dried, the temperature is raised to 90℃~100℃, and the temperature is continued for 10min~15min, so that the temperature of the fermented leaves reaches above 75℃, and the fermentation is terminated; and the chicory tea is obtained by drying at 85℃~95℃.

[0021] Preferably, the chicoric acid content in the chicory tea is 0.30 mg / mL to 0.55 mg / mL.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The present invention provides a method for producing chicory tea, comprising applying a chicory growth composition to soil where chicory grows, placing earthworm seedlings in the soil, and harvesting chicory leaves when they grow to make chicory tea; the application amount of the chicory growth composition is 79.62 g / m 2 ~477.71g / m 2 The amount of earthworm seedlings released is 1 / m 2 ~10 pieces / m 2 ; The chicory growth composition is made of the following raw materials by weight: 1 to 50 parts of nitrogen-fixing bacteria and 1 to 100 parts of natural plant fiber materials with water absorption properties. The method is to apply the chicory growth composition made of nitrogen-fixing bacteria and water-absorbing bamboo fiber to the soil where chicory grows, and put earthworm seedlings in the soil to promote the growth of chicory, and collect its leaves to prepare tea after the chicory grows moderately. The present invention considers the new material level, the microbial level and the field biological relationship level, and for the first time uses the combination of nitrogen-fixing bacteria, water-absorbing bamboo fiber materials and earthworms to increase the chicory acid content. The three components of nitrogen-fixing bacteria, water-absorbing bamboo fiber materials and earthworms are used to produce chicory tea according to the above ratio, which has a good effect on increasing the chicory acid content and significantly increases the chicory acid content.

[0024] In the present invention, the chicory growth composition provided can be better used in combination with earthworms to promote the growth of chicory and improve the quality of chicory. Fresh leaves are harvested to make chicory tea, and the selected chicory leaves are subjected to a process of drying, draining water, and rolling, which significantly reduces the bitterness and green taste of the chicory itself, changes the taste of the chicory tea, and makes the chicory tea more palatable. The fermentation process increases aromatic substances, increases the fragrance, and enhances the flavor and functionality of the chicory tea. The bitterness is reduced while the content of chicoric acid is increased, and the taste is significantly improved. It has the advantages of low cost, no environmental pollution, and is suitable for wide promotion and application, providing a new perspective for improving the quality of chicory substitute tea and increasing the content of chicoric acid. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 This is a field picture after the chicory growth composition and earthworm seedlings of the present invention are released. DETAILED DESCRIPTION

[0027] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively below, and preferred embodiments of the present invention are provided. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0029] The inventive concept of the present invention is as follows:

[0030] Chicoric acid is a type of caffeic acid derivative mainly isolated from Asteraceae plants. It is an important active ingredient in chicory, which can enhance health functions, promote the absorption of nutrients, and increase the stability and shelf life of tea. At present, how to use new materials, microbial technology and field production environment improvement to increase the content of chicoric acid in chicory tea is a difficult point in the production process of chicory tea.

[0031] Based on this, the present invention provides a method for producing chicory tea, wherein a chicory growth composition is applied to soil where chicory grows, and earthworm seedlings are placed in the soil, and chicory leaves are harvested after they grow out to make chicory tea; the application amount of the chicory growth composition is 79.62 g / m 2 ~477.71g / m 2 The amount of earthworm seedlings released is 1 / m 2 ~10 pieces / m 2 ; The chicory growth composition is made of the following raw materials by weight: 1 to 50 parts of nitrogen-fixing bacteria and 1 to 100 parts of natural plant fiber materials with water absorption properties. The nitrogen-fixing bacteria and the natural plant fiber materials with water absorption properties are applied to the soil where chicory grows in the said proportion to increase and promote the population of soil earthworms, create a good environment for the growth of chicory, increase the content of chicoric acid in chicory, and harvest fresh leaves to make chicory tea. The selected chicory leaves are subjected to a process of drying, drying and rolling to significantly reduce the bitterness and green taste of the chicory itself, change the taste of the chicory tea, and make the chicory tea more palatable. The fermentation process increases aromatic substances, increases the fragrance, and enhances the flavor and functionality of the chicory tea.

[0032] The technical solution of the present invention will be further described below in conjunction with specific embodiments.

[0033] Embodiment 1, a kind of production method of chicory tea

[0034] Raw materials preparation:

[0035] (1) Nitrogen-fixing bacteria

[0036] Sinorhizobium meliloti powder, with a Latin name of Sinorhizobium meliloti, has a content of 10 million CFU / g and is provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0037] (2) Preparation of water-absorbent bamboo fiber

[0038] The stems of Bamboo Shoots grown for 250 days at the Bamboo Sea Base in Yongfu Town, Muchuan were used as the material. After being washed with tap water, they were ground into 75 mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 15kGy, temperature of 60℃, irradiation treatment for 5min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0039] (3) Earthworms

[0040] The earthworm Pheretima aspergillum was selected, each weighing 5g.

[0041] The soil of the test land is Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2The experimental land was divided into 6 areas, of which the experimental treatment area was 20m 2 , repeated 3 times; control area 20m 2 , repeat 3 times.

[0042] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 1 part of nitrogen-fixing bacteria and 1 part of water-absorbing bamboo fiber material. The application amount of the chicory growth composition in each area was 79.62 g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 40%, soil pH is 6, and soil temperature is maintained at 20℃. 2 A trench was dug 8 cm deep, and one earthworm seedling was placed in each trench and covered with soil. In the control area, only nitrogen-fixing bacteria 60 g / m 2 No other materials were used. Each area was watered with 100 kg of water. The field pictures after the chicory growth composition and earthworm seedlings were placed were as follows: Figure 1 As shown, the chicory leaves are harvested and made into tea 15 days after they grow.

[0043] The harvested chicory leaves are spread thinly in the withering room, the temperature is kept constant at 15℃, the humidity is 65%, and they are gently turned over every hour after 5 hours. After 48 hours, the leaves are constantly picked, soft, dull in color, and have a moisture content of 55%, and the leaves are completely green and water-free.

[0044] Entering the rolling stage, the temperature in the rolling room is constant at 20℃, and the humidity is 85%. According to the light-heavy-light principle, rolling is carried out for 40 minutes, and the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 25℃, and the humidity is 90%. After fermentation for 2 hours, the chicory has a sweet aroma, the green smell dissipates, the leaf color is dark green, and the fermentation is in place. Drying is carried out by quickly raising the temperature to 90℃ with a rough fire for 10 minutes, so that the temperature of the fermented leaves reaches above 75℃, and the fermentation is terminated; then pour it into a dustpan to cool and pick out the thick old leaves. After 1 hour, put it into the drying cabinet and dry it at 85℃ to get chicory tea.

[0045] Determination of chicoric acid content:

[0046] (1) Measurement method

[0047] The content of chicoric acid was determined by high performance liquid chromatography (HPLC).

[0048] Preparation of reference solution: Take an appropriate amount of chicoric acid reference substance and add 80% methanol to make a solution containing 0.2 mg of chicoric acid per 1 mL.

[0049] Preparation of the test solution: Take 0.5 g of sample powder (passed through No. 4 sieve), place it in a stoppered conical flask, add 20 mL of 80% methanol, weigh the mass, ultrasonically treat (power is 400 W, frequency is 40 kHz) for 20 min, cool, weigh the mass again, make up the lost mass with 80% methanol, shake well, filter, and take the filtrate to obtain the test solution.

[0050] Determination of chicoric acid content: Pipette 10 μL of reference solution and test solution respectively, inject into liquid chromatograph, record the chromatogram, and calculate the content by peak area according to the external standard method.

[0051] (2) Measurement results

[0052] The chicory tea leaves made in each area were randomly sampled three times, and the chicoric acid content was determined by HPLC, and each sample was repeated three times. The research results showed that the chicoric acid content was 0.55 mg / mL. Compared with the control, the chicoric acid in the area where the chicory growth composition and earthworms were applied increased significantly, by 31.5%.

[0053] Embodiment 2, a method for producing chicory tea.

[0054] Raw materials preparation:

[0055] (1) Nitrogen-fixing bacteria

[0056] Sinorhizobium meliloti powder, with a Latin name of Sinorhizobium meliloti and a content of 20 million CFU / g, was provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0057] (2) Preparation of water-absorbent bamboo fiber

[0058] The stems of Bamboo Shoots grown for 300 days in the Bamboo Sea Base of Yongfu Town, Muchuan were used as materials. After being washed with tap water, they were ground into 80-mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 20kGy, temperature at 65℃, irradiation treatment for 6min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0059] (3) Earthworms

[0060] The earthworms (Pheretima carnosa) were selected, each weighing 5 g.

[0061] The soil of the test land is Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2 The experimental land was divided into 6 areas, of which the experimental treatment area was 20m 2 , repeated 3 times; control area 20m 2 , repeat 3 times.

[0062] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 1 part of nitrogen-fixing bacteria and 5 parts of water-absorbing bamboo fiber material. The application amount of the chicory growth composition in each area was 79.62 g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 45%, soil pH is 7, and soil temperature is maintained at 23°C. 2 A 10 cm deep trench was dug, and two earthworm seedlings were placed in each trench and covered with soil. In the control area, only nitrogen-fixing bacteria 60 g / m 2 , no other materials are used, and 100kg of water is applied to each area. The chicory leaves are harvested and made into tea 15 days after they grow out.

[0063] The harvested chicory leaves are spread thinly in the withering room, the temperature is kept constant at 15℃, the humidity is 65%, and they are gently turned over every hour after 5 hours. After 48 hours, the leaves are constantly picked, soft, dull in color, and have a moisture content of 55%, and the leaves are completely green and water-free.

[0064] Entering the rolling stage, the temperature in the rolling room is constant at 20℃, and the humidity is 85%. According to the light-heavy-light principle, rolling is carried out for 40 minutes, and the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 25℃, and the humidity is 90%. After fermentation for 2 hours, the chicory has a sweet aroma, the green smell dissipates, the leaf color is dark green, and the fermentation is in place. Drying is carried out by quickly raising the temperature to 90℃ with a rough fire for 10 minutes, so that the temperature of the fermented leaves reaches above 75℃, and the fermentation is terminated; then pour it into a dustpan to cool and pick out the thick old leaves. After 1 hour, put it into the drying cabinet and dry it at 85℃ to get chicory tea.

[0065] Determination of chicoric acid content:

[0066] The chicoric acid content was determined according to the method for determining the chicoric acid content described in Example 1. The chicoric acid content of the chicoric acid tea leaves prepared in each area was randomly sampled three times, and the chicoric acid content was determined by HPLC, and each sample was repeated three times. The research results showed that the chicoric acid content was 0.40 mg / mL. Compared with the control, the chicoric acid content in the area where the chicoric acid growth composition and earthworms were applied increased significantly, increasing by 18.3%.

[0067] Embodiment 3, a method for producing chicory tea.

[0068] Raw materials preparation:

[0069] (1) Nitrogen-fixing bacteria

[0070] Sinorhizobium meliloti powder, with a Latin name of Sinorhizobium meliloti, has a content of 10 million CFU / g and is provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0071] (2) Preparation of water-absorbent bamboo fiber

[0072] The stems of Bamboo Shoots grown for 350 days at the Bamboo Sea Base in Yongfu Town, Muchuan were used as the material. After being washed with tap water, they were ground into 85 mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 20kGy, temperature at 65℃, irradiation treatment for 6min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0073] (3) Earthworms

[0074] Shanghai Pheretima was selected, each weighing 5g.

[0075] The soil of the test land is Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2 The experimental land was divided into 6 areas, of which the experimental treatment area was 20m 2 , repeated 3 times; control area 20m 2 , repeat 3 times.

[0076] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 15 parts of nitrogen-fixing bacteria and 60 parts of water-absorbing bamboo fiber material. The application amount of the chicory growth composition in each area was 79.62 g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 50%, soil pH is 8, and soil temperature is maintained at 25℃. 2 A 12 cm deep trench was dug, and three earthworm seedlings were placed in each trench and covered with soil. In the control area, only nitrogen-fixing bacteria 60 g / m 2 , no other materials are used, and 100kg of water is applied to each area. The chicory leaves are harvested and made into tea 15 days after they grow out.

[0077] The harvested chicory leaves are spread thinly in the withering room, the temperature is kept constant at 15℃, the humidity is 65%, and they are gently turned over every hour after 5 hours. After 48 hours, the leaves are constantly picked, soft, dull in color, and have a moisture content of 55%, and the leaves are completely green and water-free.

[0078] Entering the rolling stage, the temperature in the rolling room is constant at 20℃, and the humidity is 85%. According to the light-heavy-light principle, rolling is carried out for 40 minutes, and the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 25℃, and the humidity is 90%. After fermentation for 2 hours, the chicory has a sweet aroma, the green smell dissipates, the leaf color is dark green, and the fermentation is in place. Drying is carried out by quickly raising the temperature to 90℃ with a rough fire for 10 minutes, so that the temperature of the fermented leaves reaches above 75℃, and the fermentation is terminated; then pour it into a dustpan to cool and pick out the thick old leaves. After 1 hour, put it into the drying cabinet and dry it at 85℃ to get chicory tea.

[0079] Determination of chicoric acid content:

[0080] The chicoric acid content was determined according to the method for determining the chicoric acid content described in Example 1. The chicoric acid content was determined by random sampling three times from the chicoric acid tea leaves prepared in each area using the HPLC method, and each sample was repeated three times. The results showed that the chicoric acid content was 0.49 mg / mL. Compared with the control, the chicoric acid content in the area where the chicoric acid growth composition and earthworms were applied increased significantly by 20.5%.

[0081] Embodiment 4, a method for producing chicory tea.

[0082] Raw materials preparation:

[0083] (1) Nitrogen-fixing bacteria

[0084] Sinorhizobium meliloti powder, with a Latin name of Sinorhizobium meliloti and a content of 20 million CFU / g, was provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0085] (2) Preparation of water-absorbent bamboo fiber

[0086] The stems of Bamboo Shoots grown for 300 days in the Bamboo Sea Base of Yongfu Town, Muchuan were used as materials. After being washed with tap water, they were ground into 80-mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 15kGy, temperature of 60℃, irradiation treatment for 5min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0087] (3) Earthworms

[0088] The earthworm Pheretima aspergillum was selected, each weighing 6g.

[0089] The soil of the test land is Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2 The experimental land was divided into 6 areas, of which the experimental treatment area was 20m 2 , repeated 3 times; control area 20m 2 , repeat 3 times.

[0090] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 50 parts of nitrogen-fixing bacteria and 100 parts of water-absorbing bamboo fiber material. The application rate of the chicory growth composition in each area was 300 g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 40%, soil pH is 6, and soil temperature is maintained at 20℃. 2 A trench was dug 8 cm deep, and 4 earthworm seedlings were placed in each trench and covered with soil. In the control area, only nitrogen-fixing bacteria 60 g / m 2 , no other materials are used, and 100kg of water is applied to each area. The chicory leaves are harvested and made into tea 15 days after they grow out.

[0091] The harvested chicory leaves are spread thinly in the withering room, the temperature is kept constant at 20℃, the humidity is 75%, and they are gently turned over every hour after 7.5 hours. After 48 hours, the leaves are constantly picked, soft, dull in color, and have a moisture content of 58%, and the leaves are completely green and water-free.

[0092] Entering the rolling stage, the temperature in the rolling room is constant at 24°C and the humidity is 87%. According to the light-heavy-light principle, rolling is carried out for 50 minutes until the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 26°C and the humidity is 95%. After fermentation for 2.3 hours, the chicory has a sweet aroma, the green smell dissipates, the leaf color is dark green, and the fermentation is in place. Drying is carried out by quickly raising the temperature to 95°C with a rough fire for 12 minutes to allow the temperature of the fermented leaves to reach above 75°C, and fermentation is terminated; then pour into a dustpan to cool and pick out the thick old leaves. After 1 hour, put them into the drying cabinet and dry them at 90°C to obtain chicory tea.

[0093] Determination of chicoric acid content:

[0094] The chicoric acid content was determined according to the method for determining the chicoric acid content described in Example 1. The chicoric acid content was determined by random sampling three times from the chicoric acid tea leaves prepared in each area using the HPLC method, and each sample was repeated three times. The results showed that the chicoric acid content was 0.40 mg / mL. Compared with the control, the chicoric acid content in the area where the chicoric acid growth composition and earthworms were applied increased significantly by 12.5%.

[0095] Embodiment 5, a method for producing chicory tea.

[0096] Raw materials preparation:

[0097] (1) Nitrogen-fixing bacteria

[0098] Sinorhizobium meliloti powder, with a Latin name of Sinorhizobium meliloti and a content of 20 million CFU / g, was provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0099] (2) Preparation of water-absorbent bamboo fiber

[0100] The stems of Bamboo Shoots grown for 300 days in the Bamboo Sea Base of Yongfu Town, Muchuan were used as materials. After being washed with tap water, they were ground into 80-mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 15kGy, temperature of 60℃, irradiation treatment for 5min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0101] (3) Earthworms

[0102] The earthworm Pheretima aspergillum was selected, each weighing 6g.

[0103] The soil of the test land is Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2 The experimental land was divided into 6 areas, of which the experimental treatment area was 20m 2 , repeated 3 times; control area 20m 2 , repeat 3 times.

[0104] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 1 part of nitrogen-fixing bacteria and 5 parts of water-absorbing bamboo fiber material. The application rate of the chicory growth composition in each area was 300 g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 40%, soil pH is 6, and soil temperature is maintained at 20℃. 2 A 10 cm deep trench was dug, and 6 earthworm seedlings were placed in each trench and covered with soil. In the control area, only nitrogen-fixing bacteria 60 g / m 2 , no other materials are used, and 100kg of water is applied to each area. The chicory leaves are harvested and made into tea 15 days after they grow out.

[0105] The harvested chicory leaves are spread thinly in the withering room, the temperature is kept constant at 20℃, the humidity is 75%, and they are gently turned over every hour after 7.5 hours. After 48 hours, the leaves are constantly picked, soft, dull in color, and have a moisture content of 58%, and the leaves are completely green and water-free.

[0106] Entering the rolling stage, the temperature in the rolling room is constant at 24°C and the humidity is 87%. According to the light-heavy-light principle, rolling is carried out for 50 minutes until the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 26°C and the humidity is 95%. After fermentation for 2.3 hours, the chicory has a sweet aroma, the green smell dissipates, the leaf color is dark green, and the fermentation is in place. Drying is carried out by quickly raising the temperature to 95°C with a rough fire for 12 minutes to allow the temperature of the fermented leaves to reach above 75°C, and fermentation is terminated; then pour into a dustpan to cool and pick out the thick old leaves. After 1 hour, put them into the drying cabinet and dry them at 90°C to obtain chicory tea.

[0107] Determination of chicoric acid content:

[0108] The chicoric acid content was determined according to the method for determining the chicoric acid content described in Example 1. The chicoric acid content was determined by random sampling three times from the chicoric acid tea leaves prepared in each area by HPLC, and each sample was repeated three times. The results showed that the chicoric acid content was 0.38 mg / mL. Compared with the control, the chicoric acid content in the area where the chicoric acid growth composition and earthworms were applied increased significantly by 10.3%.

[0109] Embodiment 6, a method for producing chicory tea.

[0110] Raw materials preparation:

[0111] (1) Nitrogen-fixing bacteria

[0112] Sinorhizobium meliloti powder, with a Latin name of Sinorhizobium meliloti, has a content of 10 million CFU / g and is provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0113] (2) Preparation of water-absorbent bamboo fiber

[0114] The stems of Bamboo Shoots grown for 300 days in the Bamboo Sea Base of Yongfu Town, Muchuan were used as materials. After being washed with tap water, they were ground into 80-mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 15kGy, temperature of 60℃, irradiation treatment for 5min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0115] (3) Earthworms

[0116] The earthworm Pheretima aspergillum was selected, each weighing 6g.

[0117] The soil of the test land is Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2 The experimental land was divided into 6 areas, of which the experimental treatment area was 20m 2 , repeated 3 times; control area 20m 2 , repeat 3 times.

[0118] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 1 part of nitrogen-fixing bacteria and 5 parts of water-absorbing bamboo fiber material. The application rate of the chicory growth composition in each area was 300 g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 40%, soil pH is 6, and soil temperature is maintained at 20℃. 2 A 12 cm deep trench was dug, and 8 earthworm seedlings were placed in each trench and covered with soil. In the control area, only nitrogen-fixing bacteria 60 g / m 2 , no other materials are used, and 100kg of water is applied to each area. The chicory leaves are harvested and made into tea 15 days after they grow out.

[0119] The harvested chicory leaves are spread thinly in the withering room, the temperature is kept constant at 20℃, the humidity is 75%, and they are gently turned over every hour after 7.5 hours. After 48 hours, the leaves are constantly picked, soft, dull in color, and have a moisture content of 58%, and the leaves are completely green and water-free.

[0120] Entering the rolling stage, the temperature in the rolling room is constant at 24°C and the humidity is 87%. According to the light-heavy-light principle, rolling is carried out for 50 minutes until the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 26°C and the humidity is 95%. After fermentation for 2.3 hours, the chicory has a sweet aroma, the green smell dissipates, the leaf color is dark green, and the fermentation is in place. Drying is carried out by quickly raising the temperature to 95°C with a rough fire for 12 minutes to allow the temperature of the fermented leaves to reach above 75°C, and fermentation is terminated; then pour into a dustpan to cool and pick out the thick old leaves. After 1 hour, put them into the drying cabinet and dry them at 90°C to obtain chicory tea.

[0121] Determination of chicoric acid content:

[0122] The chicoric acid content was determined by the method for determining the chicoric acid content described in Example 1. The chicoric acid content was determined by random sampling three times from the chicoric acid tea leaves prepared in each area by HPLC, and each sample was repeated three times. The results showed that the chicoric acid content was 0.36 mg / mL. Compared with the control, the chicoric acid content in the area where the chicoric acid growth composition and earthworms were applied increased significantly by 7%.

[0123] Embodiment 7, a method for producing chicory tea.

[0124] Raw materials preparation:

[0125] (1) Nitrogen-fixing bacteria

[0126] Sinorhizobium meliloti powder, with a Latin name of Sinorhizobium meliloti and a content of 20 million CFU / g, was provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0127] (2) Preparation of water-absorbent bamboo fiber

[0128] The stems of Bamboo Shoots grown for 300 days in the Bamboo Sea Base of Yongfu Town, Muchuan were used as materials. After being washed with tap water, they were ground into 80-mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 15kGy, temperature of 60℃, irradiation treatment for 5min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0129] (3) Earthworms

[0130] The earthworm Pheretima aspergillum was selected, each weighing 7g.

[0131] The soil of the test land is Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2 The experimental land was divided into 6 areas, of which the experimental treatment area was 20m 2 , repeated 3 times; control area 20m 2 , repeat 3 times.

[0132] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 1 part of nitrogen-fixing bacteria and 5 parts of water-absorbing bamboo fiber material. The application amount of the chicory growth composition in each area was 477.71 g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 40%, soil pH is 6, and soil temperature is maintained at 20℃. 2 A trench was dug 8 cm deep, and 9 earthworm seedlings were placed in each trench and covered with soil. In the control area, only nitrogen-fixing bacteria 60 g / m 2 , no other materials are used, and 100kg of water is applied to each area. The chicory leaves are harvested and made into tea 15 days after they grow out.

[0133] The harvested chicory leaves are spread thinly in the withering room, the temperature is kept constant at 26℃, the humidity is 85%, and they are gently turned over every hour after 8.5 hours. After 48 hours, the leaves are constantly picked, soft, dull in color, and have a moisture content of 60%, and the leaves are completely withered.

[0134] Entering the rolling stage, the temperature in the rolling room is constant at 26°C, and the humidity is 90%. According to the light-heavy-light principle, rolling is carried out for 55 minutes, and the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 27°C, and the humidity is 100%. After fermentation for 2.5 hours, the chicory has a sweet aroma, the green smell dissipates, the leaf color is dark green, and the fermentation is in place. Drying is carried out by quickly raising the temperature to 100°C with a rough fire for 15 minutes, so that the temperature of the fermented leaves reaches above 75°C, and the fermentation is terminated; then pour it into a dustpan to cool and pick out the thick old leaves. After 1 hour, put it into the drying cabinet and dry it at 95°C to obtain chicory tea.

[0135] Determination of chicoric acid content:

[0136] The chicoric acid content was determined according to the method for determining the chicoric acid content described in Example 1. The chicoric acid content was determined by random sampling three times from the chicoric acid tea leaves prepared in each area by HPLC, and each sample was repeated three times. The results showed that the chicoric acid content was 0.37 mg / mL. Compared with the control, the chicoric acid content in the area where the chicoric acid growth composition and earthworms were applied increased significantly by 7.3%.

[0137] Embodiment 8, a method for producing chicory tea.

[0138] Raw materials preparation:

[0139] (1) Nitrogen-fixing bacteria

[0140] Sinorhizobium meliloti powder, with a Latin name of Sinorhizobium meliloti, has a content of 10 million CFU / g and is provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0141] (2) Preparation of water-absorbent bamboo fiber

[0142] The stems of Bamboo Shoots grown for 300 days in the Bamboo Sea Base of Yongfu Town, Muchuan were used as materials. After being washed with tap water, they were ground into 80-mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 15kGy, temperature of 60℃, irradiation treatment for 5min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0143] (3) Earthworms

[0144] The earthworm Pheretima aspergillum was selected, each weighing 7g.

[0145] The soil of the test land is Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2 The experimental land was divided into 6 areas, of which the experimental treatment area was 20m 2 , repeated 3 times; control area 20m 2 , repeat 3 times.

[0146] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 1 part of nitrogen-fixing bacteria and 5 parts of water-absorbing bamboo fiber material. The application amount of the chicory growth composition in each area was 477.71 g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 40%, soil pH is 6, and soil temperature is maintained at 20℃. 2 A trench was dug 8 cm deep, 10 earthworm seedlings were placed in each trench, and then covered with soil. In the control area, only nitrogen-fixing bacteria 60 g / m 2 , no other materials are used, and 100kg of water is applied to each area. The chicory leaves are harvested and made into tea 15 days after they grow out.

[0147] The harvested chicory leaves are spread thinly in the withering room, the temperature is kept constant at 26℃, the humidity is 85%, and they are gently turned over every hour after 8.5 hours. After 48 hours, the leaves are constantly picked, soft, dull in color, and have a moisture content of 60%, and the leaves are completely withered.

[0148] Entering the rolling stage, the temperature in the rolling room is constant at 26°C, and the humidity is 90%. According to the light-heavy-light principle, rolling is carried out for 55 minutes, and the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 27°C, and the humidity is 100%. After fermentation for 2.5 hours, the chicory has a sweet aroma, the green smell dissipates, the leaf color is dark green, and the fermentation is in place. Drying is carried out by quickly raising the temperature to 100°C with a rough fire for 15 minutes, so that the temperature of the fermented leaves reaches above 75°C, and the fermentation is terminated; then pour it into a dustpan to cool and pick out the thick old leaves. After 1 hour, put it into the drying cabinet and dry it at 95°C to obtain chicory tea.

[0149] Determination of chicoric acid content:

[0150] The chicoric acid content was determined by the method for determining the chicoric acid content described in Example 1. The chicoric acid content was determined by random sampling three times from the chicoric acid tea leaves prepared in each area by HPLC, and each sample was repeated three times. The results showed that the chicoric acid content was 0.35 mg / mL. Compared with the control, the chicoric acid content in the area where the chicoric acid growth composition and earthworms were applied increased significantly by 5.6%.

[0151] Embodiment 9, a method for producing chicory tea.

[0152] Raw materials preparation:

[0153] (1) Nitrogen-fixing bacteria

[0154] Sinorhizobium meliloti powder, with a Latin name of Sinorhizobium meliloti, has a content of 10 million CFU / g and is provided by the Sichuan Key Laboratory of Bamboo Pest and Disease Control and Resource Development.

[0155] (2) Preparation of water-absorbent bamboo fiber

[0156] The stems of Bamboo Shoots grown for 300 days in the Bamboo Sea Base of Yongfu Town, Muchuan were used as materials. After being washed with tap water, they were ground into 80-mesh fine bamboo powder. 60 Co irradiation source, irradiation dose of 15kGy, temperature of 60℃, irradiation treatment for 5min, achieved directional modification of bamboo fiber, and obtained water-absorbent bamboo fiber material with 100 times higher water absorption.

[0157] (3) Earthworms

[0158] The earthworm Pheretima aspergillum was selected, each weighing 7g.

[0159] The soil of the test land is Angu Town, Leshan City, Sichuan Province. The area of ​​the test land is 120m 2 The experimental land was divided into 6 areas, of which the experimental treatment area was 20m 2 , repeated 3 times; control area 20m 2 , repeat 3 times.

[0160] The chicory growth composition was evenly spread on the soil surface of the test land. The chicory growth composition was made of the following raw materials by weight: 1 part of nitrogen-fixing bacteria and 5 parts of water-absorbing bamboo fiber material. The application amount of the chicory growth composition in each area was 477.71 g / m 2 , water each area with 100kg of water to keep the soil moist to ensure a suitable living environment for earthworms, that is, maintain the following conditions: soil moisture content is maintained at 40%, soil pH is 6, and soil temperature is maintained at 20℃. 2 A trench was dug 8 cm deep, and one earthworm seedling was placed in each trench and covered with soil. In the control area, only nitrogen-fixing bacteria 60 g / m 2 , no other materials are used, and 100kg of water is applied to each area. The chicory leaves are harvested and made into tea 15 days after they grow out.

[0161] The harvested chicory leaves are spread thinly in the withering room, the temperature is kept constant at 26℃, the humidity is 85%, and they are gently turned over every hour after 8.5 hours. After 48 hours, the leaves are constantly picked, soft, dull in color, and have a moisture content of 60%, and the leaves are completely withered.

[0162] Entering the rolling stage, the temperature in the rolling room is constant at 26°C, and the humidity is 90%. According to the light-heavy-light principle, rolling is carried out for 55 minutes, and the strips are tightly tied; take out, put in a bamboo basket and move into the fermentation room. The temperature in the fermentation room is constant at 27°C, and the humidity is 100%. After fermentation for 2.5 hours, the chicory has a sweet aroma, the green smell dissipates, the leaf color is dark green, and the fermentation is in place. Drying is carried out by quickly raising the temperature to 100°C with a rough fire for 15 minutes, so that the temperature of the fermented leaves reaches above 75°C, and the fermentation is terminated; then pour it into a dustpan to cool and pick out the thick old leaves. After 1 hour, put it into the drying cabinet and dry it at 95°C to obtain chicory tea.

[0163] Determination of chicoric acid content:

[0164] The chicoric acid content was determined according to the method for determining the chicoric acid content described in Example 1. The chicoric acid content of the chicoric acid tea leaves prepared in each area was randomly sampled three times, and the chicoric acid content was determined by HPLC, and each sample was repeated three times. The research results showed that the chicoric acid content was 0.40 mg / mL. Compared with the control, the chicoric acid content in the area where the chicoric acid growth composition and earthworms were applied increased significantly, by 15.5%.

[0165] From the above results, it can be seen that the method of improving the quality of chicory substitute tea and increasing the content of chicoric acid in this experiment has achieved significant results and successfully increased the content of chicoric acid in the experimental treatment area. Compared with the control area, the increase rate of chicoric acid in different treatment areas exceeded 5%. This result shows that the production method of chicory tea and the method of increasing the content of chicoric acid are effective and feasible.

[0166] Experimental Example 10: Effect Study

[0167] In order to improve the quality of chicory substitute tea and promote the increase of chicoric acid content, the present invention conducted a comparative test using chicory growth composition and earthworms as objects. The specific research is as follows:

[0168] The effect research experiment was carried out in the artificial climate room of the laboratory in Leshan High-tech Zone, Sichuan Province, with the temperature set and maintained at 23°C, humidity at 70%, and simulated day and night light.

[0169] Chicory is planted in a cylindrical plastic bucket with a diameter of 40 cm and a height of 30 cm. Planting conditions: soil moisture content is maintained at 40% to 50%; soil pH is 6 to 8; soil temperature is maintained at 20℃ to 25℃.

[0170] A single regression orthogonal design was adopted with 3 repetitions. The design and implementation plan are shown in Tables 1 and 2. According to the experimental research plan, nitrogen-fixing bacteria and water-absorbing bamboo fiber materials were weighed and placed on the surface of the bucket. A 10 cm hole was opened in each bucket, and earthworms were placed in the hole and covered with 5 cm of soil.

[0171] It should be noted that the top of the bucket should be sealed with gauze to prevent the earthworms from escaping.

[0172] The soil was irrigated with 30 ml of pure water every 10 days, and the soil moisture was measured every 10 days to maintain the soil moisture at 50%. The chicory leaves were harvested and made into tea 15 days after they grew out, and the chicoric acid content was investigated and the chicoric acid increase rate was calculated. The results are shown in Table 2. SPSS 20.0 data analysis software was used to perform statistical analysis and significance test on the test results.

[0173] Table 1 Orthogonal factor level design table L 9 (3 3 )

[0174]

[0175] Table 2 Orthogonal test design and results

[0176]

[0177]

[0178] It can be seen from Table 2 that the increase rate of chicoric acid content at different experimental factor levels is different, among which the level factor 1, i.e. Z1 nitrogen-fixing bacteria 10g / m 2 , Z2 water-absorbent bamboo fiber material 50g / m 2 , Z3 earthworm seedlings 8 pieces / m 2 The increase rate of chicoric acid content was the highest.

[0179] According to the data in Table 2, the regression coefficient was calculated using SPSS20.0 to establish a regression equation. Assuming that the increase rate of chicory tea on chicoric acid content is Y, and the factors affecting the increase rate are Z1 nitrogen-fixing bacteria, Z2 water-absorbing bamboo fiber, and Z3 earthworm seedlings, respectively, the regression equation is; Y = 14.0 + 6.6Z1 + 2.7Z2 + 3.3Z3.

[0180] According to the above regression equation, all regression coefficients are positive, that is, the effects of Z1 nitrogen-fixing bacteria, Z2 water-absorbing bamboo fiber, and Z3 earthworm seedlings on the increase rate and decrease rate of chicoric acid content Y are positively correlated, and the increase rate of chicoric acid content Y is the result of the joint action of the three factors. After the significance test, the effects of the three factors on Y: Z1 reached an extremely significant level of 0.01 with a P value of 0.000, Z2 and Z3 reached a significant level of 0.05, with P values ​​of 0.031 and 0.012 respectively; the regression equation was tested for significance, and the multivariate correlation coefficient between Y and Z1, Z2, and Z3 was 0.938, and the regression equation reached an extremely significant level of 0.01 with a P value of 0.000,

[0181] It can be seen that the nitrogen-fixing bacteria Z1, water-absorbing bamboo fiber Z2, and earthworm seedlings Z3 are significantly correlated with the increase rate and decrease rate of chicoric acid content, and the regression equation is true and reasonable. Therefore, the increase rate and decrease rate of chicoric acid content Y are mainly composed of three treatment effects: Z1 nitrogen-fixing bacteria, Z2 water-absorbing bamboo fiber, and Z3 earthworm seedlings.

[0182] From the above results, it can be seen that these three materials each play a unique and significant role in increasing the content rate of chicoric acid in chicory tea. The increase in chicoric acid content is related to nitrogen-fixing bacteria, water-absorbing bamboo fiber, and earthworm seedlings. Their combined use can further increase the chicoric acid content of chicory tea, providing a new idea and method for improving the function of chicory tea.

[0183] From the above results, it can be seen that the production method of chicory tea and the method for increasing the chicoric acid content provided by the present invention use nitrogen-fixing bacteria, water-absorbing bamboo fiber materials and earthworms as three components. Through repeated laboratory research and field tests, it is found that the three components of nitrogen-fixing bacteria, water-absorbing bamboo fiber materials and earthworms are used to improve the quality of chicory substitute tea and increase the content of chicoric acid, which has the advantages of good effect and low cost, no environmental pollution, and is suitable for wide promotion and application.

[0184] The present invention provides a method for producing chicory tea and a method for increasing the content of chicoric acid therein, and uses three components, namely, nitrogen-fixing bacteria, water-absorbing bamboo fiber material and earthworms, to provide a new perspective for improving the quality of chicory substitute tea and promoting the growth of probiotic chicoric acid. The method provided by the present invention enables the chicory growth composition to be better combined with earthworms to promote the growth of chicory. In addition, the method provided by the present invention can effectively increase the content of chicoric acid, providing a new perspective for improving the quality of chicory substitute tea and promoting the increase of chicoric acid content, and providing strong support for agricultural production.

[0185] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0186] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for producing chicory tea, characterized in that: The chicory growth composition is applied to the soil where chicory grows, and earthworm seedlings are placed in the soil. When chicory leaves grow out, they are harvested to make chicory tea. The application amount of the chicory growth composition is 79.62 g / m 2 ~477.71g / m 2 The amount of earthworm seedlings released is 1 / m 2 ~10 pieces / m 2 ; The chicory growth composition is prepared from the following raw materials by weight: 1 to 50 parts of nitrogen-fixing bacteria and 1 to 100 parts of natural plant fiber materials with water absorption performance.

2. The method for producing chicory tea according to claim 1, characterized in that: The chicory growth composition is prepared from the following raw materials by weight: 1 to 15 parts of nitrogen-fixing bacteria and 5 to 60 parts of natural plant fiber materials with water absorption performance.

3. The method for producing chicory tea according to claim 1, characterized in that: The earthworm seedlings are at least one of Pheretima aspergillum, Pheretima carnosa and Shanghai Pheretima.

4. The method for producing chicory tea according to claim 1, characterized in that: The natural plant fiber material with water absorption performance is a water-absorbing bamboo fiber material; The preparation method of the water-absorbing bamboo fiber material comprises the following steps: Take bamboo stems of 250-350 days old as materials, wash them, and grind them into bamboo powder of 75-85 meshes; use 60 Co irradiation source, irradiation dosage 15kGy-20kGy, temperature 60℃-65℃, irradiation treatment 5min-6min, to change the primary molecular structure of bamboo fiber, to obtain the water-absorbing bamboo fiber material.

5. The method for producing chicory tea according to claim 1, characterized in that: The nitrogen-fixing bacteria is rhizobium powder, and the concentration of live bacteria in the powder is 100 million CFU / g to 200 million CFU / g.

6. The method for producing chicory tea according to claim 1, characterized in that: Before earthworm seedlings are placed in the soil, the following conditions must be maintained: the soil moisture content is maintained at 40% to 50%; the pH value of the soil is 6 to 8; and the soil temperature is maintained at 20°C to 25°C.

7. The method for producing chicory tea according to claim 1, characterized in that: Before the earthworm seedlings are placed in the soil, they are firstly spread on the soil surface at a density of 1 earthworm / m 2 ~2 pieces / m 2 , dig trenches with a depth of 8cm to 12cm, and then place earthworm seedlings into the trenches; the number of earthworm seedlings placed in each trench is 1 to 10.

8. The method for producing chicory tea according to claim 1, characterized in that: The production process of harvesting chicory leaves to make chicory tea comprises the following steps: Spread out the fresh chicory leaves, the temperature is 15℃~26℃, the humidity is 65%~85%, the leaves are kept soft and the moisture content is 55%~60%, the leaves are ready to be green and water-free; Entering the rolling stage, the temperature is 20℃~26℃, the humidity is 85%~90%, and the rolling is carried out for 40min~55min, and the strips are tightly tied; after being taken out, ferment at a fermentation temperature of 25℃~27℃ and a humidity of 90%~100% for 2h~2.5h, and then dried, the temperature is raised to 90℃~100℃, and the temperature is continued for 10min~15min, so that the temperature of the fermented leaves reaches above 75℃, and the fermentation is terminated; and the chicory tea is obtained by drying at 85℃~95℃.

9. The chicory tea obtained by the method for producing chicory tea according to any one of claims 1 to 8, characterized in that: The chicoric acid content in the chicory tea is 0.30 mg / mL to 0.55 mg / mL.

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