A microbial type of xianglongsu biopesticide, its preparation method and application

Abstract

The application relates to a microbial type rotenone biopesticide, which comprises the following raw materials in parts by weight: 70-90 parts of a microbial fermentation liquor, 200-250 parts of a rotenone solution and 250-300 parts of a mineral source potassium fulvate carrier, the mineral source potassium fulvate is wrapped with a chitosan protective film, the microbial fermentation liquor comprises bacillus thuringiensis, and the rotenone solution comprises 40-60 wt% rotenone. The application has the effects of prolonging the storage period of the microbial type biopesticide and stabilizing the control effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biological pesticides, in particular to a microbial type of xianglaensin biological pesticide, a preparation method and application thereof. BACKGROUND

[0002] In agricultural production, pesticides used for preventing and treating pests mainly include chemical pesticides and biological pesticides, wherein the biological pesticides are highly valued due to their safe and effective characteristics and no pollution, and the use of biological pesticides to prevent and treat pests has become one of the important research topics of plant protection scientists at home and abroad.

[0003] According to the components and sources, the biological pesticides include plant source pesticides, animal source pesticides and microbial type pesticides. The microbial type pesticides have the advantages of easy degradation in the natural environment and no pollution, and become one of the important components of biological pesticides.

[0004] In view of the problems in the above-mentioned related technologies, the microbial type biological pesticides are easily affected by the external environment during storage, so that the storage period is short, which leads to unstable prevention and treatment effect, thereby limiting the popularization and use of biological pesticides. SUMMARY

[0005] In order to improve the storage period and stability of the prevention and treatment effect of the microbial type biological pesticides, the present application provides a microbial type of xianglaensin biological pesticide and a preparation method thereof.

[0006] In the first aspect, the present application provides a microbial type of xianglaensin biological pesticide, which adopts the following technical scheme:

[0007] A microbial type of xianglaensin biological pesticide, which comprises the following raw materials by weight: 70-90 parts of microbial fermentation liquor, 200-250 parts of xianglaensin solution and 250-300 parts of mineral source potassium fulvic acid carrier, the surface of the mineral source potassium fulvic acid is wrapped with a chitosan protective film, the microbial fermentation liquor comprises bacillus thuringiensis, and the xianglaensin solution comprises 40-60wt% of xianglaensin.

[0008] By adopting the above technical scheme, the microbial fermentation liquor and the xianglaensin solution are used in combination to play a synergistic role in killing insects, the mineral source potassium fulvic acid acts as a carrier for active ingredients such as the microbial fermentation liquor and the xianglaensin solution, which helps to reduce the loss of active ingredients caused by the influence of the external environment during storage of the biological pesticide, and the chitosan protective film wrapped on the surface of the mineral source potassium fulvic acid further improves the storage period and stability of the prevention and treatment effect of the biological pesticide.

[0009] Bacillus thuringiensis can secrete toxin protein, and then affect the growth and development of pests, and has good control effect on pests that harm the growth and production of crops; rotenone is an effective component extracted from the root bark of Melia toosendri, and when pests contact rotenone, the conduction of the central nervous system is blocked, the intestinal tissue and various respiratory metabolic functions are damaged, the digestion and absorption are affected, the pests refuse to eat, and the growth and development are abnormal, and the pests die.

[0010] The mineral fulvic acid potassium has a unique cavity structure, strong adsorption capacity for rotenone solution and microbial fermentation liquor, and can control the release of the active ingredients. In addition, due to the strong hydrophilicity of the mineral fulvic acid potassium, it is more helpful for the biological pesticide to penetrate into the body of pests when used, thereby achieving the effect of controlling pests.

[0011] Optionally, it comprises the following raw materials by weight: 75-85 parts of microbial fermentation liquor, 220-240 parts of rotenone solution and 260-290 parts of mineral fulvic acid potassium.

[0012] When the above technical scheme is adopted, the storage period and the stability of the control effect of the biological pesticide prepared by using 75-85 parts of microbial fermentation liquor, 220-240 parts of rotenone solution and 260-290 parts of mineral fulvic acid potassium have no obvious substantial difference. When 80 parts of microbial fermentation liquor, 230 parts of rotenone solution and 275 parts of mineral fulvic acid potassium are used, the biological pesticide prepared has the best effect.

[0013] Optionally, the surface of the mineral fulvic acid potassium is wrapped with a chitosan protective film, and the preparation method is as follows:

[0014] The chitosan is mixed with 1wt% acetic acid solution, then the mineral fulvic acid potassium is added, the amount ratio of the mineral fulvic acid potassium to the chitosan is 1:(0.8-1.2), and the mixture is treated by water bath ultrasonic for 0.8-1.2h. After cooling and drying, the mineral fulvic acid potassium wrapped with the chitosan protective film is obtained.

[0015] By adopting the above technical scheme, the chitosan is connected by intramolecular and intermolecular hydrogen bonds to form a film-forming solution, and after drying, a protective film is formed and wrapped on the surface of the mineral fulvic acid potassium. This is because the amino and hydroxyl groups on the chitosan molecules interact with the hydroxyl groups on the surface of the mineral fulvic acid potassium through hydrogen bonds to form a close combination, which protects the active ingredients adsorbed in the mineral fulvic acid potassium, prolongs the storage period of the biological pesticide and the stability of the control effect.

[0016] Optionally, the surface of the mineral fulvic acid potassium is also embedded with methionine iodine particles, and the preparation method is as follows:

[0017] The chitosan, methionine iodine particles and 1wt% acetic acid solution are mixed, then the mineral source fulvic acid potassium is added, the use amount ratio of the mineral source fulvic acid potassium, chitosan and methionine iodine particles is 1:(0.8-1.2):(0.2-0.4), the mixture is treated by water bath ultrasonic for 0.8-1.2h, and then dried after cooling to obtain the mineral source fulvic acid potassium with the methionine iodine particles embedded on the surface.

[0018] By adopting the technical scheme, the methionine iodine particles are volatile, when the biopesticide is sprayed in the field, the methionine iodine particles are gradually reduced in volume by contacting with air and being affected by humidity, the chitosan protective film becomes less dense, and many small micropores exist on the surface, so that the active ingredients in the mineral source fulvic acid potassium carrier can continuously diffuse, and the biopesticide can efficiently and durably kill pests.

[0019] Optionally, the microbial fermentation liquor further comprises the following raw materials with the following weight percentage: Bacillus thuringiensis 40-60wt%, Brevibacillus 20-30wt% and Bacillus sphaericus 20-30wt%.

[0020] By adopting the technical scheme, the Bacillus thuringiensis, Brevibacillus and Bacillus sphaericus can all secrete toxin proteins, thereby affecting the growth and development of pests and further improving the effect of pest control.

[0021] Optionally, the melioidin solution comprises the following raw materials with the following percentage: melioidin 40-60wt%, glycine 10-20wt% and methylpropylene glycol 10-20wt%.

[0022] By adopting the technical scheme, since the melioidin is not soluble in water, the melioidin solution prepared by compounding melioidin, methylpropylene glycol and glycine can promote the dispersion of melioidin, so that the melioidin is better adsorbed on the mineral source fulvic acid potassium carrier. The glycine is an amphoteric organic compound, the addition of glycine reduces the use amount of methylpropylene glycol and promotes the dispersion of melioidin, and the glycine can provide organic nitrogen for soil and provide nutrients for crops, thereby promoting the growth of crops.

[0023] In the second aspect, the application provides a preparation method of a melioidin microbial type biopesticide, which adopts the following technical scheme:

[0024] The preparation method of the melioidin microbial type biopesticide comprises the following steps:

[0025] In a sterile environment, the microbial fermentation liquor is mixed with the mineral source fulvic acid potassium carrier, then the melioidin solution is added, the mixture is mixed and dried at room temperature, and the mixture is prepared for use;

[0026] Then, the mineral source fulvic acid potassium carrier is wrapped with a chitosan protective film, and the biopesticide powder is obtained after drying.

[0027] By adopting the technical scheme, the active ingredients in the microbial fermentation liquor are protected by taking the potassium fulvate as a carrier, then the adsorption is continued by adding the meliatoxin solution, so that the active ingredients for killing pests can be more durable, in addition, the chitosan protective film can ensure the protection effect on the active ingredients, and in the process of using the biological pesticide, since the chitosan protective film is not dense, the biological pesticide can more durably kill pests, and the control effect is ensured.

[0028] In a third aspect, the application provides a use of the meliatoxin microbial type biological pesticide.

[0029] In summary, the application has at least one of the following beneficial technical effects:

[0030] 1. The application uses the microbial fermentation liquor and the meliatoxin solution in combination, which has a synergistic effect of killing pests, and the potassium fulvate is used as a carrier of the active ingredients such as the microbial fermentation liquor and the meliatoxin solution, which helps to reduce the loss of active ingredients caused by the influence of external environment during storage of the biological pesticide, and the chitosan protective film wrapped on the surface of the potassium fulvate further improves the storage period and stability of the control effect of the biological pesticide.

[0031] 2. When the microbial fermentation liquor is 75-85 parts, the meliatoxin solution is 220-240 parts, and the potassium fulvate is 260-290 parts, there is no obvious substantial difference in the storage period and stability of the control effect of the biological pesticide prepared therefrom, and when the microbial fermentation liquor is 80 parts, the meliatoxin solution is 230 parts, and the potassium fulvate is 275 parts, the biological pesticide prepared therefrom has the best effect.

[0032] 3. The chitosan protective film prepared by adding the methionine iodine particles on the surface of the carrier, when the biological pesticide is applied in the field, the methionine iodine particles gradually become smaller in volume by contacting with air and being affected by humidity, so that the chitosan protective film becomes less dense, and there are many small micropores on the surface, which facilitates the continuous diffusion of the active ingredients in the potassium fulvate carrier, so that the biological pesticide can efficiently and durably kill pests. DETAILED DESCRIPTION

[0033] The application will be further described in detail below in combination with specific examples and comparative examples.

[0034] The following raw materials in the application are all commercially available products, and they are all disclosed to fully understand the raw materials in the application, and should not be understood as limiting the source of the raw materials.

[0035] Bacillus thuringiensis, bacteria content 100 billion CFU / g; Brevibacillus brevis, bacteria content 100 billion CFU / g; Bacillus sphaericus, bacteria content 100 billion CFU / g; rotenone CAS No. 58812-37-6, particle size 120 mesh; mineral potassium fulvate carrier, particle size 60 mesh, humic acid ≥65%, fulvic acid ≥50%, potassium oxide ≥12%, pH: 8-10, purchased from Jinan Qinghai Chemical Co., Ltd.; methionine iodine granules molecular formula C5H 10 INO2S, molecular weight 275.10787, particle size 80 mesh; chitosan CAS No. 9012-76-4; glycine CAS No. 56-40-6.

[0036] Example

[0037] Examples 1.1-1.5

[0038] A rotenone microbial type of biopesticide is prepared from the following raw materials in the following amounts:

[0039] Microbial fermentation liquor 70, 75, 80, 85, 90 kg, rotenone solution 200, 220, 230, 240, 250 kg and mineral potassium fulvate carrier 250, 260, 275, 290, 300 kg;

[0040] The mineral potassium fulvate carrier is coated with a chitosan protective film, the microbial fermentation liquor includes Bacillus thuringiensis, and the rotenone solution includes 40-60wt% rotenone.

[0041] It includes the following preparation steps:

[0042] In a sterile environment, the microbial fermentation liquor (Bacillus thuringiensis) is mixed with the mineral potassium fulvate carrier, then the rotenone solution (50wt% rotenone) is added, mixed, dried at room temperature, and ready for use;

[0043] Then the mineral potassium fulvate carrier is coated with a chitosan protective film, and the preparation steps are as follows:

[0044] The chitosan is mixed with 1wt% acetic acid solution, then the above prepared mineral potassium fulvate is added, the amount of mineral potassium fulvate and chitosan is as shown in Table 1, water bath ultrasonic treatment for 1h, and dried for 5h after cooling to obtain mineral potassium fulvate coated with a chitosan protective film, thereby preparing a biopesticide powder.

[0045] As shown in Table 1, the difference between Examples 1.1-1.5 is that the amount of each raw material is different.

[0046] Table 1 Raw material usage (unit: kg) of Examples 1.1-1.5

[0047]

[0048] Examples 2.1-2.3

[0049] A microbial type of xianglaesin biopesticide differs from Example 1.3 in that the mineral fulvic acid potassium surface is also embedded with methionine iodine particles, and the preparation method is as follows:

[0050] After mixing chitosan, methionine iodine particles and 1wt% acetic acid solution, the solid-liquid ratio is 2:50, then add mineral fulvic acid potassium, the dosage ratio of mineral fulvic acid potassium and chitosan, methionine iodine particles is shown in Table 2, water bath ultrasonic treatment for 1h, after cooling and drying, get the mineral fulvic acid potassium embedded with methionine iodine particles on the chitosan protective film.

[0051] The specific dosage is shown in Table 2

[0052] Table 2 Dosage of each raw material in Examples 2.1-2.3 (unit: kg)

[0053] Example 2.1 Example 2.2 Example 2.3 Potassium fulvate 275 275 275 Chitosan 275 275 275 Methionine iodine granules 55 82.5 110

[0054] Examples 3.1-3.3

[0055] A microbial type of xianglaesin biopesticide differs from Example 2.2 in that Bacillus thuringiensis, Brevibacillus brevis and Bacillus sphaericus are added to the microbial fermentation broth, and the rest of the raw material types are exactly the same as Example 2.2.

[0056] The specific dosage is shown in Table 3.

[0057] Table 3 Dosage of each raw material in the microbial fermentation broth (unit: kg)

[0058] Example 3.1 Example 3.2 Example 3.3 Bacillus thuringiensis 40 50 60 Brevibacillus brevis 30 25 20 Bacillus sphaericus 30 25 20

[0059] Examples 4.1-4.3

[0060] A microbial type of xianglaesin biopesticide differs from Example 3.2 in that xianglaesin, glycine and methyl propylene glycol are added to the xianglaesin solution, and the rest of the raw material types are the same as Example 3.2.

[0061] The specific dosage is shown in Table 4.

[0062] Table 4 Dosage of each raw material in the xianglaesin solution (unit: kg)

[0063] Example 4.1 Example 4.2 Example 4.3 Rosmarinus officinalis 40 50 60 Glycine 10 15 20 Methylpropanediol 10 15 20

[0064] Example 5

[0065] A microbial type of xianglaesin biopesticide differs from Example 4.2 in that an equal amount of leucine is used to replace glycine.

[0066] Comparative Example 1

[0067] Comparative Example 1

[0068] The biopesticide of Comparative Example 1 is prepared in the same way as that of Example 1.3, except that the mineral source of potassium fulvate is not coated with a chitosan protective film.

[0069] Comparative Example 2

[0070] The biopesticide of Comparative Example 2 is prepared in the same way as that of Example 1.3, except that the amount of the mineral source of potassium fulvate carrier and chitosan is 0 kg.

[0071] Comparative Example 3

[0072] The biopesticide of Comparative Example 2 is prepared in the same way as that of Example 1.3, except that the amount of the mineral source of potassium fulvate carrier and chitosan is 0 kg.

[0073] Application Example

[0074] Application Examples 1.1-1.5

[0075] Application of a microbial type of biopesticide of xianglongsu, the biopesticide prepared in Examples 1.1-1.5 is stored at 30°C for 1 month, 3 months and 6 months respectively, then mixed with water and applied to the soil around the roots and the leaves of the cabbage for field experiments, the application amount of the biopesticide is 1 kg / acre.

[0076] Application Examples 2.1-2.3

[0077] Application of a microbial type of biopesticide of xianglongsu, the biopesticide prepared in Examples 2.1-2.3 is used.

[0078] Application Examples 3.1-3.3

[0079] Application of a microbial type of biopesticide of xianglongsu, the biopesticide prepared in Examples 3.1-3.3 is used.

[0080] Application Examples 4.1-4.3

[0081] Application of a microbial type of biopesticide of xianglongsu, the biopesticide prepared in Examples 4.1-4.3 is used.

[0082] Application Example 5

[0083] Application of a microbial type of biopesticide of xianglongsu, the biopesticide prepared in Example 5 is used.

[0084] Comparative Application Examples 1-3

[0085] The application of a microbial type of rotenone biopesticide, the biopesticide being prepared by the preparation of Comparative Examples 1-3.

[0086] Performance detection test

[0087] 1. The field experiment adopts a regional group test design, and the area of each region is 1 m 2 The application period is 6-7 leaves of green vegetables, and the application is carried out once every 10 days. The prevention effect is investigated after 10 days and 30 days of application, respectively.

[0088] The biopesticides of Application Examples 1.1-1.5, Application Examples 2.1-2.3, Application Examples 3.1-3.3, Application Examples 4.1-4.3, and Application Example 5 are stored at 30°C for 1 month, 3 months, and 6 months, respectively, and then field experiments are carried out, and the test results are shown in Table 5.

[0089] 2. The yield increase rate of the biopesticides of Application Examples 4.1-4.3 and Application Example 5 and Application Example 3.3 is tested, and the test results are shown in Table 6.

[0090] Table 5 Results of field application experiments of biopesticides stored for 1 month, 3 months, and 6 months

[0091]

[0092]

[0093] Table 6 Yield increase rate test results of the biopesticides of Application Examples 4.1-4.3 and Application Example 5 and Application Example 3.3

[0094] Yield-increasing rate Application Example 3.2 35.80％ Application Example 4.1 40.60％ Application Example 4.2 41.30％ Application Example 4.3 40.80％ Application Example 5 38.50％

[0095] In combination with Application Examples 1.1-1.5 and Comparative Examples 1-3 and in combination with Table 5, it can be seen that as the storage time of the biopesticide becomes longer, the prevention and control effect of Application Examples 1.1-1.5 on pests is obviously better than that of Comparative Examples 1-3, and the prevention and control durability is good, and when the dosage of each raw material of the biopesticide adopts the ratio of Application Example 1.3, the effect is better; the reason for the analysis may be that the microbial fermentation liquid and the rotenone solution are used in combination, which synergistically plays a role in killing insects, and the potassium fulvate of mineral origin acts as a carrier for the active ingredients such as the microbial fermentation liquid and the rotenone solution, which helps to reduce the loss of active ingredients caused by the influence of the external environment during storage, and the protective film of chitosan wrapped on the surface of the potassium fulvate of mineral origin further improves the storage period and the stability of the prevention and control effect of the biopesticide.

[0096] Bacillus thuringiensis can secrete toxin protein, and then affect the growth and development of pests, and has good control effect on pests that harm the growth and production of crops; rotenone is an effective component extracted from the root bark of the Chinese mahogany tree, and when pests come into contact with rotenone, the conduction of the central nervous system is blocked, the intestinal tissue and various respiratory metabolic functions are destroyed, the digestion and absorption are affected, the pests refuse to eat, leading to abnormal growth and development and death.

[0097] The mineral fulvic acid potassium has a unique cavity structure, strong adsorption capacity for rotenone solution and microbial fermentation liquor, and can control the release thereof, and due to the strong hydrophilicity of the mineral fulvic acid potassium, the penetration of the biopesticide into the body of pests is facilitated during use, thereby achieving the effect of controlling pests;

[0098] The chitosan forms a film-forming solution through intramolecular and intermolecular hydrogen bonds, and after drying, a protective film is formed and wrapped on the surface of the mineral fulvic acid potassium, which is due to the close combination of the amino groups and hydroxyl groups on the chitosan molecules and the hydroxyl groups on the surface of the mineral fulvic acid potassium through hydrogen bond interaction, thereby protecting the active ingredients adsorbed in the mineral fulvic acid potassium, prolonging the storage period of the biopesticide and the stability of the control effect.

[0099] As can be seen from the combination of application examples 2.1-2.3 and application example 1.3 and Table 5, the biopesticides of application examples 2.1-2.3 have better control effect, and the reason may be that the methionine iodine particles have volatility, when the biopesticide is applied in the field, the methionine iodine particles gradually become smaller in volume when they come into contact with air and are affected by humidity, so that the chitosan protective film becomes less dense, and there are many small micropores on the surface, which facilitates the sustained diffusion of the active ingredients in the mineral fulvic acid potassium carrier, so that the biopesticide can efficiently and durably kill pests.

[0100] As can be seen from the combination of application examples 3.1-3.3 and application example 2.2 and Table 5, when the microbial fermentation liquor uses Bacillus thuringiensis, Brevibacillus brevis and Bacillus sphaericus, as the number of microbial strains increases, the storage stability and control effect of the biopesticide are not adversely affected, and after long-term storage, the biopesticide still has better control effect.

[0101] It can be seen from the combination of application examples 4.1-4.3 and application examples 3.2, application example 5 and Tables 5-6 that the biological pesticides of application examples 4.1-4.3 are better than the control effects of application examples 3.2 and application example 5. The reason for analysis may be that the use of meliatoxin, methyl propylene glycol and glycine to obtain a meliatoxin solution can promote better dispersion of meliatoxin, so as to better adsorb on the potassium fulvic acid carrier. Glycine is an amphoteric organic compound. The addition of glycine reduces the use amount of methyl propylene glycol while promoting the dispersion of meliatoxin. Moreover, glycine can provide organic nitrogen for the soil and provide nutrients for crops, thereby promoting crop growth.

[0102] The above are preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. Therefore, any equivalent changes made on the basis of the structure, shape and principle of the present application should be covered within the protection scope of the present application.

Claims

1. A microbial type of biopesticide of toosendanin, characterized by: It comprises the following raw materials in parts by weight: 70-90 parts of microbial fermentation liquor, 200-250 parts of xianglongsin solution and 250-300 parts of potassium fulvate carrier, the surface of the potassium fulvate is wrapped with chitosan protective film, the microbial fermentation liquor comprises Bacillus thuringiensis, and the xianglongsin solution comprises 40-60 wt% of xianglongsin.

2. A microbial type biopesticide of Meliacine according to claim 1, characterized in that: It comprises the following raw materials in parts by weight: 75-85 parts of microbial fermentation liquor, 220-240 parts of xianglongsin solution and 260-290 parts of potassium fulvate.

3. The microbial type biologic pesticide of aspidin according to claim 1, characterized in that: The surface of the potassium fulvate is wrapped with chitosan protective film, and the preparation method is as follows: After mixing chitosan with 1 wt% acetic acid solution, then adding potassium fulvate, the use amount ratio of the potassium fulvate to chitosan is 1: (0.8-1.2), water bath ultrasonic treatment for 0.8-1.2 h, cooling and drying to obtain potassium fulvate wrapped with chitosan protective film.

4. The microbial type biologic pesticide of aspidin according to claim 3, characterized by: The surface of the potassium fulvate is also embedded with methionine iodine particles, and the preparation method is as follows: After mixing chitosan, methionine iodine particles and 1 wt% acetic acid solution, then adding potassium fulvate, the use amount ratio of the potassium fulvate to chitosan and methionine iodine particles is 1: (0.8-1.2): (0.2-0.4), water bath ultrasonic treatment for 0.8-1.2 h, cooling and drying to obtain potassium fulvate embedded with methionine iodine particles.

5. The microbial type biologic pesticide of Melia azedarach according to claim 1, characterized in that: The microbial fermentation liquor further comprises the following raw materials in wt%: 40-60 wt% of Bacillus thuringiensis, 20-30 wt% of Brevibacillus brevis and 20-30 wt% of Bacillus sphaericus.

6. The microbial type biologic pesticide of aspidin according to claim 1, characterized in that: The xianglongsin solution comprises the following raw materials in wt%: 40-60 wt% of xianglongsin, 10-20 wt% of glycine and 10-20 wt% of methylpropylene glycol.

7. A method for preparing the microbial-type biopesticide of toosendanin according to any one of claims 1 to 6, characterized in that, The following steps are included: In a sterile environment, mix the microbial fermentation liquor with the potassium fulvate carrier, then add the xianglongsin solution, mix well, dry at room temperature, and reserve; Then wrap the surface of the potassium fulvate carrier with chitosan protective film, and dry to obtain the biological pesticide powder.

8. Application of the xianglongsin microbial type biological pesticide of any one of claims 1-6 in field pest control.

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