Compound probiotic fermented traditional Chinese medicine feed additive and preparation method thereof

Abstract

The invention belongs to the technical field of feed additives, and particularly relates to a compound probiotic fermented traditional Chinese medicine feed additive and a preparation method thereof. The preparation method of the feed additive comprises the following steps: a, weighing the following raw materials in parts by weight: 52-68 parts of a traditional Chinese medicine composition, 0.5-2 parts of compound probiotics, 1.5-3.5 parts of compound trace elements, 0.1-0.5 part of ligninase, 2-6 parts of brown sugar and 25-40 parts of water; b, preparation of an activated bacterial liquid: uniformly mixing the compound probiotics, the compound trace elements, ligninase, brown sugar and water according to the weight ratio, and performing closed culture to obtain the activated bacterial liquid; c, preparing the compound probiotic fermented traditional Chinese medicine feed additive: crushing and uniformly mixing the traditional Chinese medicine composition to obtain a fermented raw material; and uniformly mixing a fermentation raw material with the activated bacterial liquid obtained in the step b, and fermenting to obtain the feed additive. The feed additive can promote the growth of piglets and improve the immunity of the piglets.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and particularly relates to a composite probiotic fermented traditional Chinese medicine feed additive and a preparation method thereof. Background Art

[0002] Pigs have different requirements for nutrition and energy at different growth and development stages. The growth and development stage after weaning and before fattening is usually called the piglet period. The piglet period is a critical period for the bone development and muscle growth of pigs. The growth function is vigorous and the growth rate is fast, so a large amount of nutrition is needed. However, at the same time, piglets in this period have problems such as poor immunity due to the incomplete development of various tissues and organs of the body, weak digestive system function, and incomplete development of the immune system. Therefore, careful feeding is required to accelerate the rapid growth of piglets, thereby effectively shortening the growth cycle of pigs and achieving the goal of early market release.

[0003] Feed is an indispensable and important part of contemporary animal husbandry. By adding some feed additives with specific functions to the feed, it is possible to achieve the effects of regulating nutritional balance, preventing diseases, and improving health. Prior art CN113100342A is an additive for promoting the growth of piglets and its preparation method, which discloses the use of a Chinese medicine composition as a feed additive to promote the growth of piglets. Prior art CN113061547A is a composite probiotic preparation for reducing the incidence of diarrhea in piglets, and its preparation method and application, which discloses that the composite probiotic agent compounded by lactic acid Pediococcus PA-19, plant lactobacillus P-8, and animal Bifidobacterium lactis subspecies V9 has the effect of improving piglet immunity and reducing the incidence of diarrhea. However, the above-mentioned feed additives have the problems of piglet growth rate and immunity improvement not being significant. Therefore, it is necessary to further explore the growth performance of piglets to accelerate the growth and development speed of piglets and enhance piglet immunity. Summary of the invention

[0004] The first purpose of the present invention is to provide a method for preparing a composite probiotic fermented Chinese medicinal feed additive, which is simple and easy to operate.

[0005] The second purpose of the present invention is to provide a composite probiotic fermented Chinese medicine feed additive, which can promote the growth of piglets and improve the immune ability of piglets.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is:

[0007] A method for preparing a composite probiotic fermented Chinese medicinal feed additive comprises the following steps:

[0008] a. Weigh the raw materials: in parts by weight, weigh 52-68 parts of the Chinese medicine composition, 0.5-2 parts of the compound probiotics, 1.5-3.5 parts of the compound trace elements, 0.1-0.5 parts of ligninase, 2-6 parts of brown sugar, and 25-40 parts of water;

[0009] b. Preparation of activated bacterial solution: According to the weight ratio, the composite probiotics, composite trace elements, ligninase, brown sugar, and water are evenly mixed, and after closed culture, the activated bacterial solution is obtained;

[0010] c. Preparation of a composite probiotic fermented Chinese medicine feed additive: crushing the Chinese medicine composition and mixing it evenly to obtain a fermentation raw material; mixing the fermentation raw material and the activated bacterial solution of step b evenly and fermenting them to obtain a composite probiotic fermented Chinese medicine feed additive.

[0011] Wherein, the composite probiotics in step a are composed of Bacillus subtilis and Lactobacillus fermentation grx08; the mass ratio of Bacillus subtilis to Lactobacillus fermentation grx08 is 1:(1.5-2).

[0012] Furthermore, in parts by weight, the Chinese medicine composition in step a comprises the following raw materials in parts by weight: 1-8 parts of Ginkgo biloba, 1-10 parts of Clerodendrum ternata, and 5-15 parts of pomegranate peel.

[0013] Furthermore, the viable count of the Bacillus subtilis is (1.5-3)×10 9 CFU / g, the viable count of the fermented lactobacillus grx08 is (2-3)×10 11 CFU / g.

[0014] Furthermore, the composite trace elements in step a are composed of copper sulfate, ferrous sulfate and zinc sulfate; the mass ratio of the copper sulfate, ferrous sulfate and zinc sulfate is (1-3): (1-4): (3-5).

[0015] Furthermore, the activity of the ligninase in step a is 8-10 U / mg.

[0016] Furthermore, the temperature of the closed culture in step b is 35-37° C., and the time of the closed culture is 22-24 hours.

[0017] Furthermore, in step c, the fermentation temperature is 35-40° C., the fermentation humidity is 35-45%, and the fermentation time is 2-4 days.

[0018] A composite probiotic fermented traditional Chinese medicine feed additive is prepared by adopting the preparation method.

[0019] Compared with the prior art, the beneficial effects of the present invention are mainly:

[0020] The composite probiotic fermented Chinese medicine feed additive formula of the present invention contains raw materials such as Chinese medicine composition and composite probiotics, takes the Chinese medicine composition as a fermentation substrate, and adopts composite probiotics to ferment the Chinese herbal medicine substrate. Wherein the Chinese medicine composition includes components such as butterfly pea flower and ginkgo lithospermum, and the synergistic effect of the two can better play the efficacy of the Chinese medicine composition. The composite probiotics are composed of bacillus subtilis and lactobacillus fermentation grx08, and the two synergistically enhance the effect, which can promote the fermentation of the Chinese medicine composition, i.e., the fermentation raw material, so that the product can regulate the gastrointestinal balance of piglets, promote nutrient absorption, and then promote the growth of piglets, and improve the immunity of piglets. DETAILED DESCRIPTION

[0021] The technical scheme of the present invention is further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only used to illustrate the present invention and should not be regarded as limiting the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0022] The Bacillus subtilis in the present invention is an existing strain, with a preservation number of CICC NO.10071, and is commercially available; the Lactobacillus fermentum grx08 is an existing strain, with a preservation number of CGMCC NO.7695, and is commercially available.

[0023] Example 1

[0024] Example 1 provides a method for preparing a composite probiotic fermented Chinese medicinal feed additive, the specific process is as follows:

[0025] a. Weigh the raw materials: by weight, weigh 60 parts of the Chinese medicine composition, 1 part of the compound probiotics, 2 parts of the compound trace elements, 0.3 parts of ligninase, 4 parts of brown sugar, and 31 parts of water.

[0026] In parts by weight, the Chinese medicine composition is composed of 5 parts of ginkgo lithospermum, 7 parts of butterfly pea flowers, and 12 parts of pomegranate peels;

[0027] The composite probiotics are composed of Bacillus subtilis and Lactobacillus fermentum grx08 in a mass ratio of 1:1.8, and the number of viable bacteria of Bacillus subtilis is 2×10 9 CFU / g, the number of viable bacteria of Lactobacillus fermentum grx08 was 2.5×10 11 CFU / g;

[0028] The above-mentioned composite trace elements are composed of copper sulfate, ferrous sulfate and zinc sulfate in a mass ratio of 2:2.5:4;

[0029] The activity of the above ligninase is 9U / mg.

[0030] b. Preparation of activated bacterial solution: According to the weight ratio, the composite probiotics, composite trace elements, ligninase, brown sugar and water are evenly mixed, and the activated bacterial solution is obtained after closed culture at 36° C. for 23 hours.

[0031] c. Preparation of compound probiotic fermented Chinese medicine feed additive: clean the Chinese medicine composition, crush it, pass it through a 60-mesh sieve and mix it evenly to obtain a fermentation raw material; mix the fermentation raw material evenly with the activated bacterial solution in step b, and ferment it in an anaerobic sealed environment at a temperature of 36°C and a humidity of 40% for 3 days to obtain a compound probiotic fermented Chinese medicine feed additive.

[0032] Example 1 also provides a composite probiotic fermented Chinese medicinal feed additive, which is prepared by the above method.

[0033] Example 2

[0034] Example 2 provides a method for preparing a composite probiotic fermented Chinese medicinal feed additive, the specific process is as follows:

[0035] a. Weigh the raw materials: by weight, weigh 52 parts of Chinese medicine composition, 0.5 parts of compound probiotics, 1.5 parts of compound trace elements, 0.1-0.5 parts of ligninase, 2 parts of brown sugar, and 25 parts of water.

[0036] In parts by weight, the Chinese medicine composition is composed of 1 part of Ginkgo erythrorhizon, 1 part of butterfly pea flower, and 5 parts of pomegranate peel;

[0037] The composite probiotics are composed of Bacillus subtilis and Lactobacillus fermentum grx08 in a mass ratio of 1:1.5, and the number of viable bacteria of Bacillus subtilis is 1.5×10 9 CFU / g, the number of viable bacteria of Lactobacillus fermentum grx08 is 2×10 11 CFU / g;

[0038] The above-mentioned composite trace elements are composed of copper sulfate, ferrous sulfate and zinc sulfate in a mass ratio of 1:1:3;

[0039] The activity of the above ligninase is 8U / mg.

[0040] b. Preparation of activated bacterial solution: According to the weight ratio, the composite probiotics, composite trace elements, ligninase, brown sugar and water are evenly mixed, and the activated bacterial solution is obtained after closed culture at 35° C. for 24 hours.

[0041] c. Preparation of compound probiotic fermented Chinese medicine feed additive: clean the Chinese medicine composition, crush it, pass it through a 60-mesh sieve and mix it evenly to obtain a fermentation raw material; mix the fermentation raw material evenly with the activated bacterial solution in step b, and ferment it in an anaerobic sealed environment at a temperature of 35°C and a humidity of 35% for 4 days to obtain a compound probiotic fermented Chinese medicine feed additive.

[0042] Example 2 also provides a composite probiotic fermented Chinese medicinal feed additive, which is prepared using the above method.

[0043] Example 3

[0044] Example 3 provides a method for preparing a composite probiotic fermented Chinese medicine feed additive, the specific process is as follows:

[0045] a. Weigh the raw materials: by weight, weigh 68 parts of Chinese medicine composition, 2 parts of compound probiotics, 3.5 parts of compound trace elements, 0.5 parts of ligninase, 6 parts of brown sugar, and 40 parts of water.

[0046] In parts by weight, the Chinese medicine composition is composed of 8 parts of ginkgo lithospermum, 10 parts of butterfly pea flowers, and 15 parts of pomegranate peels;

[0047] The above-mentioned composite probiotics are composed of Bacillus subtilis and Lactobacillus fermentum grx08 in a mass ratio of 1:2, and the number of viable bacteria of Bacillus subtilis is 3×10 9 CFU / g, the number of viable bacteria of Lactobacillus fermentum grx08 was 3×10 11 CFU / g;

[0048] The above-mentioned composite trace elements are composed of copper sulfate, ferrous sulfate and zinc sulfate in a mass ratio of 3:4:5;

[0049] The activity of the above ligninase is 10U / mg.

[0050] b. Preparation of activated bacterial solution: According to the weight ratio, the composite probiotics, composite trace elements, ligninase, brown sugar and water are evenly mixed, and the activated bacterial solution is obtained after closed culture at 37° C. for 22 hours.

[0051] c. Preparation of a composite probiotic fermented Chinese medicine feed additive: The Chinese medicine composition is cleaned, crushed, and mixed evenly after passing through a 60-mesh sieve to obtain a fermentation raw material; the fermentation raw material is mixed evenly with the activated bacterial solution of step b, and anaerobically sealed and fermented for 2 days at a temperature of 40°C and a humidity of 45% to obtain a composite probiotic fermented Chinese medicine feed additive.

[0052] Example 3 also provides a composite probiotic fermented Chinese medicinal feed additive, which is prepared by the above method.

[0053] Comparative Example 1

[0054] Comparative Example 1 provides a feed additive, which is different from Example 1 in that:

[0055] The Chinese medicine composition consists of 7 parts of butterfly pea flowers and 12 parts of pomegranate peels in parts by weight.

[0056] Comparative Example 2

[0057] Comparative Example 2 provides a feed additive, which is different from Example 1 in that:

[0058] The Chinese medicine composition is composed of 12 parts of butterfly pea flowers and 12 parts of pomegranate peels by weight.

[0059] Comparative Example 3

[0060] Comparative Example 3 provides a feed additive, which is different from Example 1 in that:

[0061] In parts by weight, the traditional Chinese medicine composition consists of 5 parts of ginkgo lithospermum and 12 parts of pomegranate peel.

[0062] Comparative Example 4

[0063] Comparative Example 4 provides a feed additive, which is different from Example 1 in that:

[0064] In parts by weight, the Chinese medicine composition consists of 12 parts of ginkgo lithospermum and 12 parts of pomegranate peel.

[0065] Comparative Example 5

[0066] Comparative Example 5 provides a feed additive, which is different from Example 1 in that:

[0067] The probiotics only contain Bacillus subtilis, and the amount of Bacillus subtilis is consistent with the total amount of Bacillus subtilis and Lactobacillus fermentum grx08 in Example 1.

[0068] Test example

[0069] The feed additives of Examples 1-3 and Comparative Examples 1-5 were subjected to animal tests.

[0070] Experimental subjects: 180 healthy weaned piglets of Duroc were randomly divided into 9 groups, 20 pigs in each group.

[0071] Experimental groups: the control group was fed with a basal diet; the experimental groups (Example 1-3 groups and Comparative Example 1-5 groups) were fed with a basal diet + 0.5% of each feed additive prepared in Example 1-3 and Comparative Example 1-5, and the feeding was continued for 14 days; during the feeding period, each pig had free access to drinking water.

[0072] (1) Growth performance

[0073] The weight of the piglets was recorded on the 0th and 14th days of the experiment, and the feed intake and diarrhea of ​​the piglets were recorded. At the end of the experiment, the daily weight gain, daily feed intake, feed-to-meat ratio, and diarrhea rate of the piglets were calculated. The diarrhea rate = total diarrhea times of piglets in each group / (total number of piglets in each group × number of experimental days) × 100%. The growth performance test results are shown in Table 1.

[0074] (2) Immune index detection

[0075] On the 14th day of the experiment, 10 piglets were selected from each group, 10 mL of blood was collected from the anterior vena cava, and the plasma and serum were separated by centrifugation. The levels of IgA, IgG and IgM in the serum of the piglets were detected using a kit. The test results of each group were the average of the test results of the 10 piglets. The results are shown in Table 2.

[0076] Table 1

[0077] Feed Additives / Growth Indicators Average daily weight gain (g) Average daily feed intake (g) Feed to meat ratio Diarrhea rate (%) Control group 259 421 1.63 3.21 Example 1 355 488 1.37 0.36 Example 2 352 485 1.38 0.36 Example 3 347 480 1.38 0.71 Comparative Example 1 310 463 1.49 1.43 Comparative Example 2 321 470 1.46 1.07 Comparative Example 3 302 455 1.51 2.14 Comparative Example 4 284 436 1.54 2.50 Comparative Example 5 275 428 1.56 2.86

[0078] Table 2

[0079]

[0080]

[0081] It can be seen from Table 1-2 that the feed additives prepared in Examples 1-3 can improve the average daily weight gain and immune capacity of piglets, and reduce the feed-to-meat ratio and diarrhea rate.

[0082] Compared with the feed additive group obtained by feeding Example 1-3, the various growth performance indexes and immune indexes of the feed additive piglets obtained by feeding Comparative Examples 1-4 are reduced. Wherein, the Chinese medicine composition in the feed additive formula of Comparative Example 1 does not add the ginkgo lithospermum component; the Chinese medicine composition in the feed additive formula of Comparative Example 2 does not add the ginkgo lithospermum component, but increases the dosage of the butterfly pea flower component; the Chinese medicine composition in the feed additive formula of Comparative Example 3 does not add the butterfly pea flower component; the Chinese medicine composition in the feed additive formula of Comparative Example 4 does not add the butterfly pea flower component, but increases the dosage of the ginkgo lithospermum component. The above results show that the synergistic effect of butterfly pea flower and ginkgo lithospermum can better play the efficacy of the Chinese medicine composition.

[0083] Compared with the feed additive group prepared by feeding Examples 1-3, only Bacillus subtilis was added to the feed additive formula of Comparative Example 5, and the growth performance indexes and immune indexes of the piglets fed with the feed additive prepared by Comparative Example 5 were reduced. From the above, it can be seen that the Bacillus subtilis and Lactobacillus fermentum grx08 in the composite probiotics synergistically enhance the fermentation of the Chinese medicine composition, i.e., the fermentation raw material, so that the product can regulate the gastrointestinal balance of piglets, promote nutrient absorption, and then promote the growth of piglets and improve the immune ability of piglets.

[0084] In summary, the feed additive of the present invention can promote the growth of piglets, improve the immunity of piglets, and prevent the quality of weaned piglets from being reduced due to diarrhea.

[0085] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.

Claims

1. A method for preparing a composite probiotic fermented Chinese medicinal feed additive, characterized in that: The following steps are involved: a. Weigh the raw materials: in parts by weight, weigh 52-68 parts of the Chinese medicine composition, 0.5-2 parts of the compound probiotics, 1.5-3.5 parts of the compound trace elements, 0.1-0.5 parts of ligninase, 2-6 parts of brown sugar, and 25-40 parts of water; b. Preparation of activated bacterial solution: According to the weight ratio, the composite probiotics, composite trace elements, ligninase, brown sugar, and water are evenly mixed, and after closed culture, the activated bacterial solution is obtained; c. Preparation of a composite probiotic fermented Chinese medicine feed additive: crushing the Chinese medicine composition and mixing it evenly to obtain a fermentation raw material; mixing the fermentation raw material with the activated bacterial solution of step b and then fermenting it to obtain a composite probiotic fermented Chinese medicine feed additive; Wherein, the composite probiotics in step a are composed of Bacillus subtilis and Lactobacillus fermentation grx08; the mass ratio of Bacillus subtilis to Lactobacillus fermentation grx08 is 1:(1.5-2).

2. The method for preparing the composite probiotic fermented Chinese medicinal feed additive according to claim 1, characterized in that: In parts by weight, the Chinese medicine composition in step a comprises the following raw materials in parts by weight: 1-8 parts of ginkgo lithospermum, 1-10 parts of butterfly pea flowers, and 5-15 parts of pomegranate peels.

3. The method for preparing the composite probiotic fermented Chinese medicinal feed additive according to claim 1, characterized in that: The viable bacterial count of the Bacillus subtilis is (1.5-3)×10 9 CFU / g, the viable count of the fermented lactobacillus grx08 is (2-3)×10 11 CFU / g.

4. The method for preparing the composite probiotic fermented Chinese medicinal feed additive according to claim 1, characterized in that: The composite trace elements in step a are composed of copper sulfate, ferrous sulfate and zinc sulfate; the mass ratio of the copper sulfate, ferrous sulfate and zinc sulfate is (1-3): (1-4): (3-5).

5. The method for preparing the composite probiotic fermented Chinese medicinal feed additive according to claim 1, characterized in that: The activity of the ligninase in step a is 8-10 U / mg.

6. The method for preparing the composite probiotic fermented Chinese medicinal feed additive according to claim 1, characterized in that: The temperature of the closed culture in step b is 35-37° C., and the time of the closed culture is 22-24 hours.

7. The method for preparing the composite probiotic fermented Chinese medicinal feed additive according to claim 1, characterized in that: In step c, the fermentation temperature is 35-40° C., the fermentation humidity is 35-45%, and the fermentation time is 2-4 days.

8. A composite probiotic fermented Chinese medicinal feed additive, characterized in that: The preparation method is adopted according to any one of claims 1 to 7.

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