Instant polyunsaturated fatty acid phospholipid microcapsule powder as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of microcapsules, and discloses instant polyunsaturated fatty acid phospholipid microcapsule powder and a preparation method and application thereof.The instant polyunsaturated fatty acid phospholipid microcapsule powder comprises an embedding substance and an embedded substance, and the embedding substance comprises resistant dextrin, soybean protein isolate and polysaccharide; the embedded substance is fatty acid phospholipid, the addition amount of the polysaccharide is 1%-10% of the total mass of the resistant dextrin and the soybean protein isolate, the molecular weight of the polysaccharide is smaller than 1000 Daltons, and the embedded substance and the embedding substance are mixed, emulsified, homogenized and dried in proportion. The polyunsaturated fatty acid phospholipid microcapsule powder has a quick dissolving effect, can be completely dissolved within 5 seconds, expands the application range of the polyunsaturated fatty acid phospholipid microcapsule powder, and can be applied to health-care products and functional foods.

Description

Technical Field

[0001] The invention relates to the technical field of microcapsules, and in particular to a quick-dissolving polyunsaturated fatty acid phospholipid microcapsule powder and a preparation method and application thereof. Background Art

[0002] Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), α-linolenic acid (ALA), etc. are a class of polyunsaturated fatty acids with physiological activity, which can regulate the body's lipid metabolism and immune function, and have multiple functions such as anti-cancer, prevention and treatment of cardiovascular diseases, promotion of body growth and development, and regulation of gene expression. At present, the raw materials of polyunsaturated fatty acid products sold on the market are mainly triglyceride type. Existing studies have shown that compared with ethyl ester type and triglyceride type, phospholipid type polyunsaturated fatty acids are easier to pass through the blood-brain barrier and have higher bioavailability, so they have attracted the attention of consumers.

[0003] Currently, the polyunsaturated fatty acid oil product preparations sold on the market are mainly in the form of soft capsules, compressed candies and solid beverages. Soft capsules and compressed candies will cause chewing and swallowing difficulties for children and the elderly; solid beverages made from polyunsaturated fatty acid oil microcapsule powder as raw materials have the main disadvantages of being difficult to dissolve and having low polyunsaturated fatty acid oil content; in addition, there are also reports of some direct drinking powders and soft candy products containing polyunsaturated fatty acid oils, but due to poor solubility, they lead to poor taste experience and a granular feel.

[0004] The existing technology focuses on the embedding rate, taste masking and stability of polyunsaturated fatty acid oils, and pays little attention to solubility! This limits the types of preparations containing polyunsaturated fatty acid oil products. In view of this, it is of great significance and prospect to provide instant polyunsaturated fatty acid oil microcapsule powder, especially instant polyunsaturated fatty acid phospholipid microcapsule powder, on the premise of meeting the embedding rate, taste masking and stability. Summary of the invention

[0005] In order to overcome the shortcomings and deficiencies of the prior art, the primary purpose of the present invention is to provide a fast-dissolving polyunsaturated fatty acid phospholipid microcapsule powder.

[0006] Another object of the present invention is to provide a method for preparing instant polyunsaturated fatty acid phospholipid microcapsule powder.

[0007] Another object of the present invention is to provide an application of the instant polyunsaturated fatty acid phospholipid microcapsule powder.

[0008] The purpose of the present invention is achieved through the following technical solutions:

[0009] A quick-dissolving polyunsaturated fatty acid phospholipid microcapsule powder, comprising an embedding substance and an embedded substance;

[0010] The embedding material includes resistant dextrin, soy protein isolate and polysaccharide;

[0011] The embedded substance is a fatty acid phospholipid, preferably a polyunsaturated fatty acid phospholipid;

[0012] The added amount of the polysaccharide is 1% to 10% of the total mass of the resistant dextrin and the isolated soy protein.

[0013] Preferably, the mass ratio of the embedded substance to the embedding substance is 1-2:1;

[0014] The mass ratio of the resistant dextrin to the isolated soy protein is 1-3:1.

[0015] Preferably, the polysaccharide is derived from plant polysaccharides, microbial polysaccharides or a mixture thereof, preferably at least one of Lentinan, Poria cocos polysaccharide and Hericium erinaceus polysaccharide;

[0016] The molecular weight of the polysaccharide is less than 1000 Daltons, preferably less than 500 Daltons.

[0017] Preferably, the embedded material is prepared from soybean lecithin and polyunsaturated fatty acids by immobilized enzyme catalysis, wherein the mass content of polyunsaturated fatty acids is 40-70%, preferably 60-70%.

[0018] Preferably, the dissolution time of the instant polyunsaturated fatty acid phospholipid microcapsule powder is less than 5 seconds.

[0019] Preferably, the instant polyunsaturated fatty acid phospholipid microcapsule powder is prepared by mixing the embedded substance and the embedding substance in proportion, and subjecting the mixture to emulsification, homogenization and drying.

[0020] A method for preparing instant polyunsaturated fatty acid phospholipid microcapsule powder comprises the following steps:

[0021] (1) Mix the embedding materials in proportion and add water to dissolve them into a solution of a certain concentration;

[0022] (2) weighing a certain amount of the embedded substance, adding it to the solution in step (1), and dispersing it into an emulsion;

[0023] (3) homogenizing the emulsion obtained in step (2) under certain homogenizing conditions and then drying to obtain a powder;

[0024] (4) Screening the powder obtained in step (3) to obtain instant polyunsaturated fatty acid phospholipid microcapsule powder.

[0025] Preferably, the mass concentration of the solution in step (1) is 5%-30%, preferably 10%-20%.

[0026] Preferably, the homogenization conditions in step (3) are as follows: the homogenization pressure is 20-50 MPa, preferably 30-40 MPa; the homogenization times are 2-7 times, preferably 3-5 times; and the homogenization temperature is 0-20°C, preferably 2-10°C.

[0027] Preferably, the drying in step (3) is spray drying or freeze drying, preferably freeze drying.

[0028] Preferably, the pore size of the sieving in step (4) is 30-150 mesh, preferably 80-120 mesh, and the proportion of particles passing through 80 mesh is less than 10%.

[0029] The instant polyunsaturated fatty acid phospholipid microcapsule powder is used in the preparation of nutritional supplements, health products and functional foods.

[0030] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0031] 1) The present invention uses resistant dextrin, soy protein isolate and polysaccharide as the embedding materials of the polyunsaturated fatty acid phospholipid microcapsules, which have a quick-dissolving effect and can be completely dissolved within 5 seconds, and also have a good taste-masking effect;

[0032] 2) Resistant dextrin is a low-calorie water-soluble dietary fiber, and the prepared polyunsaturated fatty acid phospholipid microcapsule powder has better nutritional value. DETAILED DESCRIPTION

[0033] The present invention is further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto. For process parameters not particularly noted, conventional techniques may be used.

[0034] In order to demonstrate that the instant polyunsaturated fatty acid phospholipid microcapsule powder of the present invention has excellent instant solubility and also has a good taste masking effect, the polyunsaturated fatty acid phospholipids in the examples are uniformly selected as DHA type phospholipids, wherein the DHA content is 65wt.%, but the embodiments of the present invention are not limited thereto.

[0035] Example 1

[0036] (1) Weigh 20 g of resistant dextrin, 10 g of soy protein isolate and 3 g of lentinan with a molecular weight of less than 1000 Dalton as embedding materials, with the amount of lentinan added being 10%, where the amount added refers to the percentage of polysaccharide in the total mass of resistant dextrin and soy protein isolate, stir evenly and add water to make a solution with a mass concentration of 10%;

[0037] (2) Add 33 g of polyunsaturated fatty acid phospholipid (phospholipid with a DHA content of 65%) to the solution prepared in (1), with the mass ratio of polyunsaturated fatty acid phospholipid to embedding material being 1:1, and disperse the mixture in a disperser at 10,000 r / min for 15 min to form a uniform emulsion;

[0038] (3) homogenizing the emulsion prepared in step (2) for 3 times at a homogenizing pressure of 35 MPa and a homogenizing temperature of 10° C., and then drying the emulsion by freeze drying;

[0039] (4) The microcapsule powder dried in step (3) was sieved to collect 80-120 mesh powder to prepare instant polyunsaturated fatty acid phospholipid microcapsule powder sample 1.

[0040] Example 2

[0041] (1) Weigh 20 g of resistant dextrin, 10 g of soy protein isolate and 0.3 g of Lentinan with a molecular weight of less than 500 Dalton as embedding materials, with the Lentinan added in an amount of 1%, stir evenly and add water to make a solution with a mass concentration of 10%;

[0042] (2) Add 36.36 g of polyunsaturated fatty acid phospholipid (phospholipid with a DHA content of 65 wt.%) to the solution prepared in (1), with the mass ratio of polyunsaturated fatty acid phospholipid to encapsulating material being 1.2:1, and disperse into a uniform emulsion using a disperser at 10,000 r / min for 15 min;

[0043] (3) homogenizing the emulsion prepared in step (2) for 3 times at a homogenizing pressure of 30 MPa and a homogenizing temperature of 10° C., and then drying the emulsion by freeze drying;

[0044] (4) The microcapsule powder dried in step (3) was sieved to collect 80-120 mesh powder to prepare instant polyunsaturated fatty acid phospholipid microcapsule powder sample 2.

[0045] Example 3

[0046] (1) Weigh 20 g of resistant dextrin, 20 g of soy protein isolate and 2 g of Lentinan with a molecular weight of less than 500 Dalton as embedding materials, with the Lentinan added in an amount of 5%, stir evenly and add water to make a solution with a mass concentration of 10%;

[0047] (2) Add 63 g of polyunsaturated fatty acid phospholipid (phospholipid with a DHA content of 65 wt.%) to the solution prepared in (1), with the mass ratio of polyunsaturated fatty acid phospholipid to embedding material being 1.5:1, and disperse the mixture in a disperser at 10,000 r / min for 15 min to form a uniform emulsion;

[0048] (3) homogenizing the emulsion prepared in step (2) twice at a homogenizing pressure of 35 MPa and a homogenizing temperature of 10° C., and then drying the emulsion by freeze drying;

[0049] (4) The microcapsule powder dried in step (3) was sieved to collect 80-120 mesh powder to prepare instant polyunsaturated fatty acid phospholipid microcapsule powder sample 3.

[0050] Example 4

[0051] (1) Weigh 25 g of resistant dextrin, 10 g of soy protein isolate and 2.5 g of tuckahoe polysaccharide with a molecular weight of less than 1000 Dalton as embedding materials, with the tuckahoe polysaccharide added in an amount of 7.14%, stir evenly and add water to make a solution with a mass concentration of 10%;

[0052] (2) Add 27.5 g of polyunsaturated fatty acid phospholipid (phospholipid with a DHA content of 65 wt.%) to the solution prepared in (1), with the mass ratio of polyunsaturated fatty acid phospholipid to embedding material being 1:1, and disperse the mixture at 10,000 r / min for 20 min to form a uniform emulsion;

[0053] (3) homogenizing the emulsion prepared in step (2) for 3 times at a homogenizing pressure of 35 MPa and a homogenizing temperature of 2° C., and then drying the emulsion by freeze drying;

[0054] (4) The microcapsule powder dried in step (3) was sieved to collect 80-120 mesh powder to prepare instant polyunsaturated fatty acid phospholipid microcapsule powder sample 4.

[0055] Example 5

[0056] (1) Weigh 30 g of resistant dextrin, 10 g of soy protein isolate and 2 g of Hericium erinaceus polysaccharide with a molecular weight of less than 1000 Dalton as embedding materials, with the Hericium erinaceus polysaccharide added in an amount of 5%, stir evenly and add water to make a solution with a mass concentration of 10%;

[0057] (2) Add 54.6 g of polyunsaturated fatty acid phospholipid (phospholipid with a DHA content of 65 wt.%) to the solution prepared in (1), the mass ratio of polyunsaturated fatty acid phospholipid to embedding material being 1.3:1, and disperse the mixture at 10,000 r / min for 18 min to form a uniform emulsion using a disperser;

[0058] (3) homogenizing the emulsion prepared in step (2) for 3 times at a homogenizing pressure of 35 MPa and a homogenizing temperature of 2° C., and then drying the emulsion by freeze drying;

[0059] (4) The microcapsule powder dried in step (3) was sieved to collect 80-120 mesh powder to prepare instant polyunsaturated fatty acid phospholipid microcapsule powder sample 4.

[0060] Comparative Example 1

[0061] The preparation method of this comparative example is the same as that of Example 1, except that:

[0062] Comparative Example Sample 1 was obtained by replacing resistant dextrin with maltodextrin.

[0063] Comparative Example 2

[0064] The preparation method of this comparative example is the same as that of Example 1, except that:

[0065] The amount of polysaccharide added was 0, and comparative example sample 2 was obtained.

[0066] Test Case

[0067] Determination of dissolution time of polyunsaturated fatty acid phospholipid microcapsule powder:

[0068] The stabilized phospholipid DHA microcapsules obtained in the examples and comparative examples were evaluated for their fishy smell intensity and stability tested. The stability test conditions were: at 37° C. and 75% relative humidity for 3 months, and the peroxide value and malondialdehyde content were measured.

[0069] Evaluation of fishy smell intensity: The fishy smell intensity is scored on a 7-point scale (0 for absence, 1 for presence, 2 for mild, 3 to 4 for moderate, 4 to 5 for heavy, and 7 for very heavy). The average score of the 30 evaluators is the fishy smell intensity.

[0070] Table 1 Odor intensity evaluation test results

[0071] sample initial 37℃, 75%RH, 3 months later Example 1 0 0 Example 2 0 1 Example 3 0 0 Example 4 0 0 Example 5 0 1 Comparative Example 1 2 4 Comparative Example 2 2 5

[0072] 5 g of the samples of the embodiments and comparative examples were respectively weighed and dissolved in 500 mL of distilled water (25° C.), and the time required for the samples to be completely dissolved was measured under magnetic stirring at 500 rpm / min.

[0073] Table 2 Dissolution time determination results of polyunsaturated fatty acid phospholipid microcapsule powder

[0074]

[0075]

[0076] The polyunsaturated fatty acid phospholipid microcapsule powder of the present invention can be completely dissolved within 5 seconds and has a good taste masking effect, which is better than the comparative example.

[0077] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A quick-dissolving polyunsaturated fatty acid phospholipid microcapsule powder, characterized in that: The soluble polyunsaturated fatty acid phospholipid microcapsule powder comprises an embedding substance and an embedded substance; The embedding material includes resistant dextrin, soy protein isolate and polysaccharide; The embedded substance is fatty acid phospholipid; The added amount of the polysaccharide is 1% to 10% of the total mass of the resistant dextrin and the isolated soy protein.

2. The instant polyunsaturated fatty acid phospholipid microcapsule powder according to claim 1, characterized in that: The mass ratio of the embedded substance to the embedding substance is 1-2:1; The mass ratio of the resistant dextrin to the isolated soy protein is 1-3:

1.

3. The instant polyunsaturated fatty acid phospholipid microcapsule powder according to claim 1, characterized in that: The polysaccharide is a plant polysaccharide or a microbial polysaccharide or a mixture thereof; The molecular weight of the polysaccharide is less than 1000 Daltons.

4. The instant polyunsaturated fatty acid phospholipid microcapsule powder according to claim 1, characterized in that: The embedded material is prepared from soybean lecithin and polyunsaturated fatty acid by catalysis of immobilized enzyme, wherein the mass content of polyunsaturated fatty acid is 40-70%.

5. The method for preparing the instant polyunsaturated fatty acid phospholipid microcapsule powder according to any one of claims 1 to 4, characterized in that: The steps include: (1) Mix the embedding materials in proportion and add water to dissolve them into a solution of a certain concentration; (2) weighing a certain amount of the embedded substance, adding it to the solution in step (1), and dispersing it into an emulsion; (3) homogenizing the emulsion obtained in step (2) under certain homogenizing conditions and then drying to obtain a powder; (4) Screening the powder obtained in step (3) to obtain instant polyunsaturated fatty acid phospholipid microcapsule powder.

6. The method for preparing the instant polyunsaturated fatty acid phospholipid microcapsule powder according to claim 5, characterized in that: The mass concentration of the solution in step (1) is 5%-30%.

7. The method for preparing the instant polyunsaturated fatty acid phospholipid microcapsule powder according to claim 5, characterized in that: The homogenization conditions in step (3) are: homogenization pressure is 20-50 MPa, homogenization times are 2-7 times, and homogenization temperature is 0-20°C.

8. The method for preparing the instant polyunsaturated fatty acid phospholipid microcapsule powder according to claim 5, characterized in that: The drying in step (3) is spray drying or freeze drying, preferably freeze drying.

9. The method for preparing the instant polyunsaturated fatty acid phospholipid microcapsule powder according to claim 5, characterized in that: The pore size of the screening in step (4) is 30-150 mesh.

10. Use of the instant polyunsaturated fatty acid phospholipid microcapsule powder according to any one of claims 1 to 4 in the preparation of nutritional supplements, health products and functional foods.

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