Enteric-coated sustained-release gel particles for oral beauty treatment and oxidation resistance, and preparation method and application of enteric-coated sustained-release gel particles

Abstract

The invention belongs to the technical field of peptide, new materials and food processing, and discloses an enteric-coated slow-release gel particle for oral beauty treatment and oxidation resistance, and a preparation method and application of the enteric-coated slow-release gel particle. The collagen peptide gel particles comprise the following raw material components in parts by weight: 3-6 parts of collagen peptide, 1 part of vitamin C, 1-2 parts of sodium alginate, 0.5-1.5 parts of a thickening agent, 0.8-1.2 parts of an emulsifying agent, 2-4 parts of food-grade calcium salt and purified water. An oil-in-water method is adopted to prepare the enteric sustained-release gel particles, through a unique sustained-release system, various colloids are compounded, and the optimal proportion of the addition amount of each colloid is controlled, the prepared gel particles are high in water content, high in biodegradability and small in irritation, and can be matched with matched drinks, the whole taste is improved, and meanwhile, the taste of the gel particles is improved. And direct swallowing can be realized, the outer layer protection material is prevented from being damaged by chewing, the collagen peptide is effectively protected from being damaged by gastric juice, and the collagen peptide is ensured to safely reach the intestinal tract and be efficiently absorbed and utilized, so that the efficacy of the product is remarkably improved.

Description

Technical Field

[0001] The invention belongs to the technical fields of peptides, new materials and food processing, and in particular to enteric-coated sustained-release gel particles for oral beauty and anti-oxidation, and a preparation method and application thereof. Background Art

[0002] Oral beauty refers to a beauty method that improves skin condition, maintains skin health and beauty by taking specific foods or nutrients orally. Compared with topical skin care products, oral beauty products have outstanding advantages and are safer. They usually do not need to act directly on the skin, so they can reduce irritation and damage to the skin; oral beauty products can be absorbed through the intestines and have a holistic effect. They can not only improve skin condition, but also improve the overall health of the body; the effects of oral beauty products are usually more lasting because they can regulate the body from the inside and improve the internal environment of the skin. The main ingredients of oral beauty products on the market currently include collagen and antioxidants such as vitamins, β-carotene, and minerals.

[0003] Aging caused by ultraviolet radiation is called photoaging, and its clinical symptoms include the appearance of wrinkles, loss of elasticity, dryness, roughness, capillary dilation, epidermal hyperplasia and sebaceous gland hyperplasia. In the process of skin aging, it is often accompanied by changes in the structure and function of the extracellular matrix of the dermis. Collagen is mainly produced by fibroblasts in the dermis, providing good elasticity for the skin. In recent years, preventing and treating photoaging has become one of the research hotspots, especially the anti-skin aging effect of oral collagen hydrolysate has been widely reported. Its mechanism of action includes effectively inhibiting matrix metalloproteinases (MMPs), enhancing antioxidant activity, improving epidermal hyperplasia, and increasing the content of water, hyaluronic acid and collagen in the skin.

[0004] Collagen is a biological macromolecular substance and the most abundant protein in the human body, accounting for more than 30% of the total protein in the body. For the skin, it can give the skin firmness and elasticity, reduce wrinkles, and is the key to maintaining the youthful state of the skin. Under physiological conditions, collagen can be decomposed by non-specific proteases in the gastrointestinal tract, and finally absorbed in the form of short peptides (dipeptides, tripeptides, etc.) with smaller molecular weight and free amino acids. Scientific research has found that the bioavailability of oral collagen in rats is 57.8%, and 63.4% of collagen is absorbed by the intestine in the form of peptides. Collagen peptides are a polypeptide chain formed by hydrolysis and digestion of collagen molecules. The absorption principle of collagen peptides is similar to that of other proteins, and it is mainly absorbed by the intestine and transported into the blood through the circulatory system. Collagen peptides are mainly hydrolyzed into smaller polypeptides and amino acids in the stomach. When collagen peptides enter the stomach, they are digested by gastric acid and pepsin. Pepsin exerts its hydrolysis effect in an acidic environment and breaks down collagen peptides into smaller molecular fragments. These smaller peptides and amino acids can be more easily absorbed through the intestinal wall. On the small intestinal epithelial cells, there are many protein transporters and peptidases that can further transport peptides and amino acids from the intestinal cavity into the cells for further decomposition and utilization within the cells.

[0005] Antioxidants are a class of substances that can help capture and neutralize free radicals, thereby eliminating the damage caused by free radicals to the human body. Antioxidants work by neutralizing free radicals. Free radicals are highly active molecules or atomic groups that can damage biological macromolecules such as cell membranes and DNA, thereby accelerating the aging process of cells. Antioxidants can capture these free radicals and convert them into harmless substances, thereby protecting cells from damage. There are many types of antioxidants, and those commonly used in functional foods such as oral beauty include: vitamins, such as vitamin C, vitamin E, and vitamin A; polyphenols, such as tea polyphenols, anthocyanins (grape seed extract, etc.), resveratrol, etc.; carotenoids, such as β-carotene, lycopene, etc.; minerals, such as trace elements such as selenium, zinc, and copper.

[0006] Gel particles are an advanced drug or nutritional supplement delivery system designed to improve the effective utilization and stability of the product by controlling the release rate and location of the ingredients. Gel particles are generally multi-layered, each layer has different functions, and its structure usually includes the following layers. Outer layer: generally a gastric acid-resistant polymer material layer, alginate and Ca 2＋Chemical cross-linking occurs under acidic conditions, and the resulting calcium alginate cross-linked three-dimensional eggshell structure can protect the embedded material from being destroyed by gastric juice, resist the erosion of gastric acid and bile, and protect the internal components from passing through the digestive tract. Middle layer: usually a protective layer of plant materials, such as β-cyclodextrin, which has the function of isolating water and oxygen. Inner layer: a sustained-release layer for active ingredients, ensuring that the active ingredients are released and implanted in the appropriate position in the intestine, thereby improving the utilization rate of the product.

[0007] Through searching, similar products widely sold on the market currently generally have larger particle sizes designed, and are mainly suitable for swallowing after chewing. However, this design feature is not conducive to swallowing the product directly with beverages. Specifically, if the product is taken by chewing, it is likely to destroy the gastric acid-resistant protective material on the outer layer of the product. The particle size design of existing products and their administration methods have certain limitations and shortcomings when used directly with beverages.

[0008] Through searching, the existing gel particle preparation methods generally use deacetylated chitosan (also known as chitosan) as the wall material to ensure the stability and mechanical properties of gel particles. Under acidic conditions, the amino group is protonated to have cationic properties and can be cross-linked with negatively charged substances. However, according to the GB 2760-2024 Food Additives Usage Standard, the only varieties of chitosan allowed to be used are Western ham (smoked, smoked, and boiled ham) and meat sausage. Therefore, the food industry generally uses single colloids in edible gums such as gelatin, pectin, and xanthan gum to replace chitosan. If only a single polymer material is used as a carrier to prepare gel particles, there are shortcomings such as low embedding rate and poor stability, and thus often show poor mechanical properties.

[0009] Through searching, it is found that most of the current products on the market are collagen peptide drinks. On the one hand, high-concentration collagen peptides will retain some of the original fishy smell, and the collagen peptides need to be mixed with other ingredients such as flavors and spices to mask the fishy smell to a certain extent. These added ingredients will affect the purity and taste of the collagen peptides; on the other hand, when collagen peptide drinks are ingested, after the collagen peptides enter the stomach, they will be gradually broken down into smaller polypeptides and amino acids under the action of gastric acid and pepsin.

[0010] Through searching, no patent publication documents related to the patent application of the present invention have been found. Summary of the invention

[0011] The purpose of the present invention is to overcome the deficiencies in the prior art and provide an enteric-coated sustained-release gel particle for oral beauty and anti-oxidation, and a preparation method and application thereof.

[0012] The technical solution adopted by the present invention to solve its technical problem is:

[0013] Disclosed are enteric-coated sustained-release gel particles for oral beauty and anti-oxidation. The raw material components and weight proportions of the gel particles are: 3-6 parts of collagen peptide, 1 part of vitamin C, 1-2 parts of sodium alginate, 0.5-1.5 parts of thickener, 0.8-1.2 parts of emulsifier, 2-4 parts of food-grade calcium salt and purified water.

[0014] Furthermore, the collagen peptide is any one or more of bonito elastin peptide, tilapia collagen peptide, cod collagen peptide, rousselot collagen peptide, and collagen tripeptide.

[0015] Furthermore, the viscosity of the sodium alginate is 600-800 mPa·s.

[0016] Furthermore, the thickener is any one or more of sodium carboxymethyl cellulose, hydroxypropyl distarch phosphate, xanthan gum, carrageenan, gelatin, pectin, guar gum, and gellan gum.

[0017] Furthermore, the emulsifier is any one or more of Tween 40, Span 60, polyethylene glycol, glycerol, modified soybean lecithin, sodium caseinate, phospholipids, fatty acid glyceride, propylene glycol alginate, propylene glycol, and mannitol.

[0018] Furthermore, the food-grade calcium salt is any one or more of calcium chloride, calcium lactate, and calcium carbonate.

[0019] The preparation method of the enteric-coated sustained-release gel particles for oral beauty and anti-oxidation as described above comprises the following steps:

[0020] (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use;

[0021] (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use;

[0022] (3) Mix the thickener, emulsifier and purified water at a material-water ratio of 1:14.6 g / ml, heat in a water bath at 85°C to dissolve the composite adhesive solution, and keep it warm for later use;

[0023] (4) Mix food-grade calcium salt with purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use;

[0024] (5) Mix the sodium alginate solution of step (2) and the composite adhesive solution of step (3), heat the mixture in a water bath at 85° C. to obtain a sodium alginate composite adhesive solution, and keep the mixture warm for later use;

[0025] The sodium alginate composite gel solution in step (5) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the gel particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After being fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

[0026] The use of the gel particles as described above in improving the absorption of collagen peptides in the intestine.

[0027] The advantages and positive effects achieved by the present invention are:

[0028] 1. The present invention adopts the oil-in-water method to prepare enteric-coated sustained-release gel particles. Through a unique sustained-release system, multiple colloids are compounded and the optimal ratio of the added amount of each colloid is controlled, so that the prepared gel particles have high water content, strong biodegradability, and low irritation. They can be combined with matching beverages to improve the overall taste while being directly swallowed to avoid chewing and damaging the outer protective material, effectively protecting the collagen peptides from being damaged by gastric juice, ensuring that they safely reach the intestines and are efficiently absorbed and utilized, thereby significantly improving the efficacy of the product.

[0029] 2. The present invention embeds collagen peptides and other effective ingredients through the oil-in-water method, and while achieving an enteric-coated sustained-release effect, obtains a gel particle with high water content, strong biodegradability, and low irritation, which can play a role in human oral beauty, reducing water loss, improving skin elasticity, and anti-oxidation.

[0030] 3. The present invention verifies the protective effect of gel particles encapsulating collagen peptides on photoaged mice by studying the effects of gel particles encapsulating collagen peptides on the water content, hyaluronic acid content, and hydroxyproline content of the back skin of photoaged mice; by comparing the effects of gel particles prepared under different encapsulation systems, it is proved that the present invention can exert the optimal efficacy of collagen peptides by compounding multiple colloids and controlling the ratio of each colloid. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a spherical morphology diagram of the enteric-coated sustained-release gel particles of the present invention after heat resistance testing;

[0032] Figure 2 This is a graph showing the hydroxyproline content in mouse skin in the present invention;

[0033] Figure 3 This is a graph showing the hyaluronic acid content in mouse skin in the present invention;

[0034] Figure 4 This is a graph showing the water content of mouse skin in the present invention. DETAILED DESCRIPTION

[0035] The present invention will be further described below in conjunction with the embodiments. The following embodiments are descriptive rather than restrictive, and the protection scope of the present invention cannot be limited by the following embodiments.

[0036] The various experimental operations involved in the specific embodiments are all routine techniques in the art. For parts not specially annotated in this document, ordinary technicians in the art can implement them by referring to various commonly used reference books, scientific and technological literature or related instructions, manuals, etc. before the filing date of this invention.

[0037] Disclosed are enteric-coated sustained-release gel particles for oral beauty and anti-oxidation. The raw material components and weight proportions of the gel particles are: 3-6 parts of collagen peptide, 1 part of vitamin C, 1-2 parts of sodium alginate, 0.5-1.5 parts of thickener, 0.8-1.2 parts of emulsifier, 2-4 parts of food-grade calcium salt and purified water.

[0038] Preferably, the collagen peptide is any one or more of bonito elastin peptide, tilapia collagen peptide, cod collagen peptide, rousselot collagen peptide, and collagen tripeptide.

[0039] Preferably, the viscosity of the sodium alginate is 600-800 mPa·s.

[0040] Preferably, the thickener is any one or more of sodium carboxymethyl cellulose, hydroxypropyl distarch phosphate, xanthan gum, carrageenan, gelatin, pectin, guar gum, and gellan gum.

[0041] Preferably, the emulsifier is any one or more of Tween 40, Span 60, polyethylene glycol, glycerol, modified soybean lecithin, sodium caseinate, phospholipids, fatty acid glycerides, propylene glycol alginate, propylene glycol, and mannitol.

[0042] Preferably, the food-grade calcium salt is any one or more of calcium chloride, calcium lactate, and calcium carbonate.

[0043] The preparation method of the enteric-coated sustained-release gel particles for oral beauty and anti-oxidation as described above comprises the following steps:

[0044] (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use;

[0045] (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use;

[0046] (3) Mix the thickener, emulsifier and purified water at a material-water ratio of 1:14.6 g / ml, heat in a water bath at 85°C to dissolve the composite adhesive solution, and keep it warm for later use;

[0047] (4) Mix food-grade calcium salt with purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use;

[0048] (5) Mix the sodium alginate solution of step (2) and the composite adhesive solution of step (3), heat the mixture in a water bath at 85° C. to obtain a sodium alginate composite adhesive solution, and keep the mixture warm for later use;

[0049] The sodium alginate composite gel solution in step (5) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the gel particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After being fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

[0050] The use of the gel particles as described above in improving the absorption of collagen peptides in the intestine.

[0051] Specifically, the relevant preparation and detection are as follows:

[0052] Example 1

[0053] Disclosed are enteric-coated sustained-release gel particles for oral beauty and anti-oxidation, comprising the following raw materials in parts by weight: 1 part of bonito elastin peptide, 1 part of tilapia collagen peptide, 1 part of cod collagen peptide, 1 part of Rousselot collagen peptide, 1 part of collagen tripeptide, 1 part of vitamin C, 1 part of sodium alginate, 1 part of pectin, 1 part of gelatin, 1 part of carrageenan, 1 part of gellan gum, 2 parts of glycerol, 3 parts of calcium chloride, 2 parts of calcium lactate, and an appropriate amount of purified water.

[0054] The preparation method of the above-mentioned enteric-coated sustained-release gel particles for oral beauty and anti-oxidation comprises the following steps:

[0055] (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use;

[0056] (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use;

[0057] (3) Mix pectin, gelatin, carrageenan, gellan gum, glycerin and purified water at a material-water ratio of 1:14.6 g / ml, heat in a water bath at 85°C to dissolve to obtain a composite glue solution, and keep warm for later use;

[0058] (4) Mix calcium chloride, calcium lactate and purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use;

[0059] (5) Mix the sodium alginate solution of step (2) and the composite adhesive solution of step (3), heat the mixture in a water bath at 85° C. to obtain a sodium alginate composite adhesive solution, and keep the mixture warm for later use;

[0060] The sodium alginate composite gel solution in step (5) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the gel particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After being fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

[0061] Example 2

[0062] Disclosed are enteric-coated sustained-release gel particles for oral beauty and anti-oxidation, comprising the following raw materials in parts by weight: 1 part of bonito elastin peptide, 1 part of tilapia collagen peptide, 1 part of cod collagen peptide, 1 part of Rousselot collagen peptide, 1 part of collagen tripeptide, 1 part of vitamin C, 1 part of sodium alginate, 1 part of sodium hydroxymethyl cellulose, 1 part of gelatin, 1 part of carrageenan, 1 part of gellan gum, 2 parts of glycerol, 3 parts of calcium chloride, 2 parts of calcium lactate, and an appropriate amount of purified water.

[0063] The preparation method of the above-mentioned enteric-coated sustained-release gel particles for oral beauty and anti-oxidation is the same as that in Example 1.

[0064] Example 3

[0065] Disclosed are enteric-coated sustained-release gel particles for oral beauty and anti-oxidation, comprising the following raw materials in parts by weight: 1 part of bonito elastin peptide, 1 part of tilapia collagen peptide, 1 part of cod collagen peptide, 1 part of Rousselot fish collagen peptide, 1 part of collagen tripeptide, 1 part of vitamin C, 1 part of sodium alginate, 1 part of sodium hydroxymethyl cellulose, 1 part of pectin, 1 part of gellan gum, 2 parts of glycerol, 3 parts of calcium chloride, 2 parts of calcium lactate, and an appropriate amount of purified water.

[0066] The preparation method of the above-mentioned enteric-coated sustained-release gel particles for oral beauty and anti-oxidation is the same as that in Example 1.

[0067] Example 4

[0068] Disclosed are enteric-coated sustained-release gel particles for oral beauty and anti-oxidation, comprising the following raw materials in parts by weight: 1 part of bonito elastin peptide, 1 part of tilapia collagen peptide, 1 part of cod collagen peptide, 1 part of Rousselot collagen peptide, 1 part of collagen tripeptide, 1 part of vitamin C, 1 part of sodium alginate, 1 part of hydroxypropyl distarch phosphate, 1 part of konjac flour, 1 part of xanthan gum, 1 part of carrageenan, 1 part of gellan gum, 2 parts of glycerol, 3 parts of calcium chloride, 2 parts of calcium lactate, and an appropriate amount of purified water.

[0069] The preparation method of the above-mentioned enteric-coated sustained-release gel particles for oral beauty and anti-oxidation comprises the following steps:

[0070] (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use;

[0071] (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use;

[0072] (3) Mix hydroxypropyl distarch phosphate, konjac flour, xanthan gum, carrageenan, gellan gum, glycerin and purified water at a material-water ratio of 1:14.6 g / ml, heat in a water bath at 85°C to dissolve to obtain a composite glue solution, and keep warm for later use;

[0073] (4) Mix calcium chloride, calcium lactate and purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use;

[0074] (5) Mix the sodium alginate solution of step (2) and the composite adhesive solution of step (3), heat the mixture in a water bath at 85° C. to obtain a sodium alginate composite adhesive solution, and keep the mixture warm for later use;

[0075] The sodium alginate composite gel solution in step (5) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the gel particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After being fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

[0076] Comparative Example 1

[0077] Compared with Example 4, the only difference is that gellan gum is not used in the raw materials, and the rest is the same as Example 4.

[0078] The preparation method of the enteric-coated sustained-release gel particles comprises the following steps:

[0079] (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use;

[0080] (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use;

[0081] (3) Mix hydroxypropyl distarch phosphate, konjac flour, xanthan gum, carrageenan, glycerin and purified water at a material-water ratio of 1:14.6 g / ml, heat in a water bath at 85°C to dissolve to obtain a composite glue solution, and keep warm for later use;

[0082] (4) Mix calcium chloride, calcium lactate and purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use;

[0083] (5) Mix the sodium alginate solution of step (2) and the composite adhesive solution of step (3), heat the mixture in a water bath at 85° C. to obtain a sodium alginate composite adhesive solution, and keep the mixture warm for later use;

[0084] The sodium alginate composite gel solution in step (5) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the gel particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After being fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

[0085] Comparative Example 2

[0086] Compared with Example 4, the only difference is that konjac flour is not used in the raw materials, and the rest is the same as Example 4.

[0087] The preparation method of the enteric-coated sustained-release gel particles comprises the following steps:

[0088] (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use;

[0089] (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use;

[0090] (3) Mix hydroxypropyl distarch phosphate, xanthan gum, carrageenan, gellan gum, glycerin and purified water at a material-water ratio of 1:14.6 g / ml, heat in a water bath at 85°C to dissolve to obtain a composite glue solution, and keep warm for later use;

[0091] (4) Mix calcium chloride, calcium lactate and purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use;

[0092] (5) Mix the sodium alginate solution of step (2) and the composite adhesive solution of step (3), heat the mixture in a water bath at 85° C. to obtain a sodium alginate composite adhesive solution, and keep the mixture warm for later use;

[0093] The sodium alginate composite gel solution in step (5) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the gel particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After being fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

[0094] Comparative Example 3

[0095] Compared with Example 4, the only difference is that carrageenan is not used in the raw materials, and the rest is the same as Example 4.

[0096] The preparation method of the enteric-coated sustained-release gel particles comprises the following steps:

[0097] (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use;

[0098] (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use;

[0099] (3) Mix hydroxypropyl distarch phosphate, konjac flour, xanthan gum, gellan gum, glycerin and purified water at a material-water ratio of 1:14.6 g / ml, heat in a water bath at 85°C to dissolve to obtain a composite glue solution, and keep warm for later use;

[0100] (4) Mix calcium chloride, calcium lactate and purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use;

[0101] (5) Mix the sodium alginate solution of step (2) and the composite adhesive solution of step (3), heat the mixture in a water bath at 85° C. to obtain a sodium alginate composite adhesive solution, and keep the mixture warm for later use;

[0102] The sodium alginate composite gel solution in step (5) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the gel particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After being fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

[0103] Comparative Example 4

[0104] Compared with Example 4, the only difference is that hydroxypropyl distarch phosphate is not used in the raw materials, and the rest is the same as Example 4.

[0105] The preparation method of the enteric-coated sustained-release gel particles comprises the following steps:

[0106] (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use;

[0107] (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use;

[0108] (3) Mix konjac flour, xanthan gum, carrageenan, gellan gum, glycerin and purified water at a material-water ratio of 1:14.6, heat in a water bath at 85°C to dissolve the composite glue solution, and keep it warm for later use;

[0109] (4) Mix calcium chloride, calcium lactate and purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use;

[0110] (5) Mix the sodium alginate solution of step (2) and the composite adhesive solution of step (3), heat the mixture in a water bath at 85° C. to obtain a sodium alginate composite adhesive solution, and keep the mixture warm for later use;

[0111] The sodium alginate composite gel solution in step (5) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the gel particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After being fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

[0112] Comparative Example 5

[0113] Compared with Example 4, the only difference is that the method of MAITYS et al. and SHUXZ et al. is referred to and slightly modified. One portion of chitosan is added to a 1% acetic acid solution to a final chitosan concentration of 1.2%, and stirred for 3 h until the chitosan is completely dissolved.

[0114] (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use;

[0115] (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use;

[0116] (3) Mix calcium chloride, calcium lactate and purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use;

[0117] (4) Mix the sodium alginate solution and chitosan solution in step (2) in a volume ratio of 1:2, heat the mixture in a water bath at 85°C to obtain a sodium alginate composite solution, and keep the mixture warm for later use;

[0118] The sodium alginate complex solution in step (4) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After the particles are fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

[0119] The relevant tests are as follows:

[0120] Test Example 1 Comparison of hardness, elasticity and chewability of enteric-coated sustained-release gel particles:

[0121] The enteric-coated sustained-release gel particles prepared in Example 1 to Example 4 and Comparative Example 1 to Comparative Example 5 were tested for hardness, elasticity and chewiness. The testing method was as follows: several gel particles prepared in the same example and the same batch were used as samples, and then a portion of the samples was taken out for "double destructive compression test". Specifically, a gel particle sample was placed in the loading area of ​​the base of the texture analyzer, and the gel particle sample was subjected to two destructive compressions using a probe to obtain hardness and elasticity parameters. The parameters were set as follows: the pre-test speed was 1.0 mm / s, the speed during the test was equal to the speed after the test, which was 2.5 mm / s, and the trigger force was 10 g; the compression degree of the first destructive compression was 50%, and the compression degree of the second destructive compression was 70%, and the interval between the two destructive compressions was 5 s. The chewiness of gel particles is a relatively subjective sensory attribute that can be evaluated through sensory evaluation. Although it is highly subjective, it can reflect consumers' true feelings about the chewiness of gel particles. The results are shown in Table 1 below. It can be seen from the results in Table 1 that the hardness, elasticity and chewiness of the enteric-coated sustained-release gel particles of Examples 1 to 4 of the present invention are very good, and it can be seen that the enteric-coated sustained-release gel particles have sufficient elasticity and toughness. Among them, the composite colloid used in Example 4 has the highest hardness and the best elasticity. The hardness and elasticity of Comparative Examples 1 to 5 are relatively poor, and the effects are significantly worse than those of Examples 1 to 4. From the experimental results, the effect of using a composite colloid is much better than that of a single colloid.

[0122] Table 1

[0123]

[0124] At the same time, by comparing Example 4 and Comparative Examples 1 to 4, it can be seen that the hydroxypropyl distarch phosphate, konjac flour, carrageenan and gellan gum in the present invention have a synergistic effect with each other and can synergistically improve the relevant properties of the prepared enteric sustained-release gel particles.

[0125] Test Example 2 Heat resistance test of enteric-coated sustained-release gel particles

[0126] Take 10g of the enteric-coated sustained-release gel particles of Examples 1 to 4 and Comparative Examples 1 to 5 in a 50ml transparent glass bottle, seal it, add 40mL of purified water, preheat at 75℃ for 1h, and observe the spherical morphology. After preheating, place it in an autoclave and sterilize it at 105℃ for 45min. Observe the spherical morphology. Figure 1, compared with the chewiness before sterilization. The results are shown in Table 2 below. It can be seen from the results in Table 2 that the enteric-coated sustained-release gel particles of Examples 1 to 4 of the present invention have good heat resistance and are more convenient to use when applied to some warm products. Among them, the hardness of Example 4 does not change much after heating, and the taste remains in a relatively good state; the enteric-coated sustained-release gel particles of Comparative Examples 1 to 5 are slightly damaged, and the effect is obviously worse than that of Examples 1 to 4 of the present invention, especially Example 4.

[0127] At the same time, by comparing Example 4 and Comparative Examples 1 to 4, it can be seen that the hydroxypropyl distarch phosphate, konjac flour, carrageenan and gellan gum in the present invention have a synergistic effect with each other and can synergistically improve the relevant properties of the prepared enteric sustained-release gel particles.

[0128] Table 2

[0129]

[0130] Test Example 3 Determination of collagen peptide embedding rate in enteric-coated sustained-release gel particles

[0131] (1) Determination of protein content in enteric-coated sustained-release gel granules by Kjeldahl method

[0132] Weigh 0.5 g of enteric-coated sustained-release gel particles of Example 1-Example 4 and Comparative Example 1-Comparative Example 5, put them at the bottom of a clean digestive tube, add 0.2 g of copper sulfate, 6.0 g of potassium sulfate, and 20 mL of concentrated sulfuric acid to the digestive tube. Use programmed temperature rise (260 ° C digestion for 30 min, 300 ° C digestion for 30 min), keep the test solution in a slightly boiling state after it becomes clear, place it on a digestion furnace for digestion for 0.5 h, wait until the liquid in the digestive tube is completely transparent and clear, cool it, and end the digestion. Add 20 mL of water, connect the distillation device, add 10 mL of 40% mass concentration sodium hydroxide solution for alkalization, heat and distill the escaped ammonia, absorb it with boric acid solution, titrate it with a standard hydrochloric acid solution, and calculate the protein content in the sample. The protein content calculation formula is:

[0133]

[0134] Where: X is the protein content in the enteric-coated sustained-release gel granules, g / 100 g; V 1 The volume of hydrochloric acid standard titration solution consumed for each test group, mL; V 2 is the volume of the standard hydrochloric acid titration solution consumed in each blank repetition, mL; c is the concentration of the standard hydrochloric acid titration solution, mol·L -1 ; 14 is the molar mass of nitrogen, g·mol -1; 6.25 is the average coefficient for converting nitrogen into crude protein; m is the mass of the weighed sample in each test group, g; V is the total volume of the sample digestion liquid, mL; V' is the volume of the digestion liquid used for distillation, mL.

[0135] (2) Determination of the proportion of collagen peptides with a relative molecular mass less than 10,000 Da

[0136] The proportion of collagen peptides with a relative molecular mass of less than 10,000 Da in the enteric-coated sustained-release gel particles was determined according to the national standard GB 31645-2018. About 125.0 mg of enteric-coated sustained-release gel particles were weighed with weighing paper, transferred to a 25 mL volumetric flask, fixed to the scale with mobile phase, ultrasonically vibrated for 10 minutes to fully dissolve and mix the sample, and filtered with a polytetrafluoroethylene filter membrane with a pore size of 0.2 μm to 0.5 μm, and the filtrate was used for determination. The chromatographic conditions were determined as follows: chromatographic column: TSKgel G2000 SWXL 300mm×7.8mm (GEL LOT 502R); mobile phase: acetonitrile: water: trifluoroacetic acid, volume ratio of 40:60:0.05; detection wavelength: 220nm; flow rate: 0.5mL / min; column temperature: 30℃; injection volume: 10µL; in order to make the chromatographic system meet the detection requirements, it is stipulated that under the above chromatographic conditions, the column efficiency of the gel chromatographic column, i.e. the theoretical plate number (N), calculated based on the tripeptide standard (tyrosine-tyrosine-tyrosine) peak, should not be less than 5000, and the distribution coefficient (Kd) of the protein peptide should be between 0 and 1.

[0137] (3) Calculation of the embedding rate of enteric-coated sustained-release gel particles

[0138] The calculation formula of collagen peptide content with a relative molecular mass less than 10,000 Da is:

[0139]

[0140] Where X is the embedding rate, m 1 is the protein content in the enteric-coated sustained-release gel particles after embedding, λ is the peak area percentage of collagen peptides with a relative molecular mass less than 10000Da in the enteric-coated sustained-release gel particles, m 2 The results are shown in Table 3 below. It can be seen from the results in Table 3 that the embedding rate of the enteric-coated sustained-release gel particles of Examples 1 to 4 of the present invention is above 96%, and the embedding rate of Comparative Examples 1 to 5 of the present invention is significantly lower than that of Examples 1 to 4. The embedding rate of the enteric-coated sustained-release gel particles of Examples 1 to 4 of the present invention is relatively high, and the gel particles made of the composite colloid selected in Example 4 have the highest embedding rate, which is about 98.28%, providing a reliable application basis for related products and ensuring the high efficiency and stability of the products.

[0141] At the same time, by comparing Example 4 and Comparative Examples 1 to 4, it can be seen that the hydroxypropyl distarch phosphate, konjac flour, carrageenan and gellan gum in the present invention have a synergistic effect with each other and can synergistically improve the relevant properties of the prepared enteric sustained-release gel particles.

[0142] Table 3

[0143]

[0144] Test Example 4 Tolerance of Enteric-coated Sustained-release Gel Granules to Artificial Gastric Juice and Artificial Intestinal Juice

[0145] (1) Simulated gastric digestion:

[0146] In vitro simulated artificial gastric fluid: After sterilizing 0.1 mol / L potassium phosphate buffer, adjust the pH to 2.5 with standard hydrochloric acid titration solution, add pepsin with a mass concentration of 10 g / L (final concentration), mix well and pass through a 0.22 μm sterile membrane to obtain in vitro simulated artificial gastric fluid.

[0147] Take 5g of enteric sustained-release gel particles of Example 1-Example 4 and Comparative Example 1-Comparative Example 5, wash three times with purified water, place in 3.0 mL, pH 2.5 simulated gastric juice environment, and simulate gastric juice digestion under anaerobic conditions at 37 ° C. Samples were taken at 1, 2, and 3 h to observe the spheroid morphology. The results are shown in Table 4 below. All samples of Example 1-Example 4 showed stable morphological characteristics at 1 hour and 2 hours, that is, no disintegration occurred, and they were slightly elastic after light compression. This shows that the sample has good structural stability and certain elastic recovery ability in the initial stage. At 3 hours, the samples of all embodiments still did not disintegrate, and there were slight differences in the deformation after light compression. The samples of the four embodiments maintained good morphological stability during the observation period, and no disintegration occurred, but the deformation after light compression increased slightly over time. For Comparative Examples 1-Comparative Examples 5, there was a slight disintegration phenomenon at 1 hour, and the disintegration was obvious until 3 hours.

[0148] The product of the present invention adopts a unique gastric acid-resistant technology, which ensures that the gel particles maintain integrity and effectiveness in the acidic environment of the stomach on the one hand, and on the other hand, the product of the present invention does not release a large amount of active ingredients in the stomach, thereby reducing irritation to the gastric mucosa and thus reducing user discomfort.

[0149] At the same time, by comparing Example 4 and Comparative Examples 1 to 4, it can be seen that the hydroxypropyl distarch phosphate, konjac flour, carrageenan and gellan gum in the present invention have a synergistic effect with each other and can synergistically improve the relevant properties of the prepared enteric sustained-release gel particles.

[0150] Table 4

[0151]

[0152] (2) Simulated intestinal digestion:

[0153] In vitro simulated artificial intestinal fluid: After sterilizing 0.1 mol / L potassium phosphate buffer, adjust the pH to 6.8 with 0.1 mol / L sodium hydroxide solution, add pancreatic enzyme with a mass concentration of 10 g / L (final concentration) and porcine bile salt with a mass concentration of 3 g / L (final concentration, that is, 1 L water plus 10 g pancreatic enzyme and 3 g porcine bile salt), mix well and pass through a 0.22 μm sterile membrane to obtain in vitro simulated artificial intestinal fluid.

[0154] Take 5g of enteric sustained-release gel particles of Example 1-Example 4 and Comparative Example 1-Comparative Example 5, wash three times with purified water, place in a simulated intestinal fluid environment of 3.0 mL and pH 6.8, and simulate intestinal fluid digestion under anaerobic conditions at 37 °C. Samples were taken at 2, 4, 6, and 8 hours to observe the spheroid morphology. The results are shown in Table 5 below. The samples of all embodiments maintained a good spherical shape at the time point of 1 hour, and no disintegration occurred. This shows that the samples have good structural integrity and stability in the initial stage. At 2 hours, the samples of all embodiments still had no disintegration phenomenon in the natural state, but disintegrated after light pressure. This shows that when the sample is subjected to a certain external force, its structural stability decreases, but a certain external force is still required to cause disintegration. At 3 hours, the samples of all embodiments disintegrated under light pressure, and obvious dehydration occurred. This shows that over time, the internal structure of the sample may have further changed, resulting in an increase in its tendency to disintegrate under external force, accompanied by the occurrence of dehydration. At 6 hours, all samples of the examples disintegrated naturally without the need for external force. Compared with the examples, Comparative Examples 1 to 5 showed poor stability in the initial stage (1 hour). Their spheres disintegrated under light pressure, accompanied by obvious dehydration. Examples 1-4 performed well in maintaining the shape and stability of the spheres, although they also disintegrated after a long time. However, Comparative Examples 1 to 5 showed poor disintegration performance and dehydration in the initial stage, and this instability became more obvious over time. Therefore, it can be considered that the effects of Comparative Examples 1 to 5 are poor.

[0155] The product of the present invention protects the active ingredients by embedding, so that they are not affected by gastric acid and digestive enzymes before reaching the intestine. This can ensure that the active ingredients are stably released in the intestine and exert the maximum effect. The collagen peptide drinks available on the market lack such a protection mechanism, and the collagen peptides are decomposed before reaching the intestine.

[0156] At the same time, by comparing Example 4 and Comparative Examples 1 to 4, it can be seen that the hydroxypropyl distarch phosphate, konjac flour, carrageenan and gellan gum in the present invention have a synergistic effect with each other and can synergistically improve the relevant properties of the prepared enteric sustained-release gel particles.

[0157] Table 5

[0158]

[0159] Experimental Example 6 Protective Effect of Collagen Peptide Gel Particles on Photoaging Mice

[0160] According to the results of the above test examples, considering the spherical morphology, hardness, collagen peptide embedding rate and other conditions, the gel particles prepared in Example 4 have the best effect, and the gel particles prepared in Comparative Examples 1-5 have similar states. Comparative Examples 1 and 5 are randomly selected as test objects.

[0161] Animal model establishment: The SPF-grade male healthy Balb / c mice used in this experiment were provided by Henan Sikebes Biotechnology Co., Ltd., with a body weight controlled between 18 and 20 g, and the license number was SCXK (Yu) 2020-0005. During the experiment, the mice were kept in a constant environment, the temperature was maintained at 20-25 ° C, the relative humidity was controlled at 50 ± 5%, and a 12-h light-dark cycle was followed. To ensure that the mice adapted to the experimental environment, they were adaptively raised for one week. 60 mice were randomly divided into 6 groups, 10 in each group, set as experimental group 1 (adding gel particles prepared in Example 4), experimental group 2 (adding gel particles prepared in Comparative Example 1), experimental group 3 (adding gel particles prepared in Comparative Example 5), experimental group 4 (equal volume of collagen peptides, consistent with the raw materials embedded in the gel particles prepared in Example 4), blank control group (UV unexposed, equal volume of deionized water treated mice), model control group (UV exposed, equal volume of deionized water treated mice). The conditions of UV exposure were as follows: 10 mice were housed in a cage with 10 small compartments to ensure that each mouse was exposed to UV light, and the distance from the UV lamp to the mouse back skin was 30 cm, five times a week for 8 weeks, and each UV exposure lasted for 20 minutes. Except for the normal group, the total dose of UV exposure was 300 mJ / cm in the first week. 2 , the second week is 600 mJ / cm 2 , the third week is 900 mJ / cm 2 , the fourth week is 1200 mJ / cm 2The gel particles were cut into a particle size sufficient for oral administration to mice. For experimental groups 1-4, the back skin (3 cm×3 cm) of the mice was depilated with 8% sodium sulfide for 24 hours after UV exposure, and then the drug was orally administered with the feed (100 mg / kg body weight, fasting for 5 hours before oral administration) two hours before UV exposure.

[0162] Determination of skin component related indicators: About 0.1g of skin tissue (excluding subcutaneous tissue) from the depilated area on the back of the mouse was taken, rinsed twice with pre-cooled saline, and then dried with filter paper. It was quickly and accurately weighed and placed in a 4 ml EP tube. The tissue blocks were cut into pieces (ice bath), and pre-cooled saline (final tissue concentration 0.1 g / ml) was added and homogenized under ice bath conditions. The supernatant was taken after centrifugation at 12000 rpm for 20 minutes. The content of hyaluronic acid (HA) and hydroxyproline (Hyp) in the supernatant was determined strictly according to the steps of the kit.

[0163] Determination of skin moisture content: Take about 0.1g of skin tissue (excluding subcutaneous tissue) from the depilated area on the back of the mouse, weigh it accurately and put it into a glass tube, bake it in an oven (105℃) for 4 hours, and then weigh it. Calculate the skin moisture content of each group of animals. The calculation formula is as follows:

[0164]

[0165] The results of skin component related indexes are as follows Figure 2 As shown. According to the experimental results, the Hyp content of the normal group was 2.92±0.72 (p <0.05). Compared with the normal group, UV exposure caused the Hyp content to be significantly reduced to 1.83±0.55 (p <0.05). The Hyp content of experimental group 1 was 2.89±0.41 (p <0.05), the Hyp content of experimental group 2 was 2.33±0.21 (p <0.05), the Hyp content of experimental group 3 was 2.35±0.43 (p <0.05), and the Hyp content of experimental group 4 was 2.08±0.22 (p <0.05). Compared with the model group, after taking collagen peptides, the Hyp content of mice increased to a certain extent, but not as significantly as the Hyp content of mice taking gel particles. Among them, experimental group 1 had the best effect, which was about 24.03% higher than experimental group 2, about 22.98% higher than experimental group 3, and about 38.94% higher than experimental group 4. Hyaluronic acid (HA) has a strong ability to bind water and has an important impact on skin moisture. HA can also support the skin and promote the diffusion of metabolites and nutrients. Figure 3It can be seen that the HA content in the skin of the model group mice irradiated by UV was 1.07±0.05, which was significantly lower than 1.86±0.14 in the blank control group (p <0.05). The HA content of experimental group 1 was 1.84±0.11 (p <0.05), the HA content of experimental group 2 was 1.27±0.09 (p <0.05), the HA content of experimental group 3 was 1.29±0.10 (p <0.05), and the HA content of experimental group 4 was 1.12±0.10 (p <0.05). Among them, experimental group 1 had the best effect, and the test results were close to those of the blank group. The above experimental data, on the one hand, show that collagen peptides help reduce the loss of collagen in UV-induced photoaging mice, and on the other hand, show that gel particles can protect most collagen peptides from gastric juice damage, allowing them to reach the intestine in larger quantities and play a role.

[0166] Skin moisture content measurement results Figure 4 As shown, according to the experimental results, the skin water content of the normal group was 62.27±3.41% (p <0.05), the skin water content of the model group was 36.47±1.28% (p <0.05), the skin water content of the experimental group 1 was 41.28±2.23 (p <0.05), the skin water content of the experimental group 2 was 46.97±1.19 (p <0.05), the skin water content of the experimental group 3 was 47.39±1.21 (p <0.05), and the skin water content of the experimental group 4 was 51.22±1.21 (p <0.05). Compared with the normal group, ultraviolet irradiation caused a significant decrease in skin moisture (p <0.05). Compared with the model group, the intake of collagen peptides and gel particles could effectively increase skin moisture content, but the experimental group 1 had the best effect.

[0167] In summary, according to the experimental results, ultraviolet irradiation can cause a significant decrease in skin moisture, HA content and Hyp content (p <0.05), while the skin moisture content, HA content and Hyp content of mice that ingested collagen peptides and gel particles can all increase, indicating that collagen peptides and gel particles are helpful in reducing the loss of water and collagen in UV-induced photoaging mice, but the experimental group 1 (i.e., the gel particles made from the composite colloid described in Example 4) is much better than the gel particles and collagen peptides made from a single colloid. It is speculated that the gel particles made in Example 4 are more resistant to gastric acid, which can ensure that more collagen peptides reach the intestine to be fully absorbed and exert their effects.

[0168] At the same time, by comparing Example 4 and Comparative Examples 1 to 4, it can be seen that the hydroxypropyl distarch phosphate, konjac flour, carrageenan and gellan gum in the present invention have a synergistic effect with each other and can synergistically improve the relevant properties of the prepared enteric sustained-release gel particles.

[0169] Although the embodiments of the present invention are disclosed for illustrative purposes, those skilled in the art will appreciate that various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims. Therefore, the scope of the present invention is not limited to the contents disclosed in the embodiments.

Claims

1. An enteric-coated sustained-release gel granule for oral beauty and anti-oxidation, characterized in that: The raw material components and weight proportions of the gel particles are: 3-6 parts of collagen peptide, 1 part of vitamin C, 1-2 parts of sodium alginate, 0.5-1.5 parts of thickener, 0.8-1.2 parts of emulsifier, 2-4 parts of food-grade calcium salt and purified water.

2. The enteric-coated sustained-release gel granules for oral beauty and anti-oxidation according to claim 1, characterized in that: The collagen peptide is any one or more of bonito elastin peptide, tilapia collagen peptide, cod collagen peptide, rousselot collagen peptide, and collagen tripeptide.

3. The enteric-coated sustained-release gel granules for oral beauty and anti-oxidation according to claim 1, characterized in that: The viscosity of the sodium alginate is 600-800 mPa·s.

4. The enteric-coated sustained-release gel granules for oral beauty and anti-oxidation according to claim 1, characterized in that: The thickener is any one or more of sodium carboxymethyl cellulose, hydroxypropyl distarch phosphate, xanthan gum, carrageenan, gelatin, pectin, guar gum, and gellan gum.

5. The enteric-coated sustained-release gel granules for oral beauty and anti-oxidation according to claim 1, characterized in that: The emulsifier is any one or more of Tween 40, Span 60, polyethylene glycol, glycerol, modified soybean lecithin, sodium caseinate, phospholipids, fatty acid glyceride, propylene glycol alginate, propylene glycol, and mannitol.

6. The enteric-coated sustained-release gel granules for oral beauty and anti-oxidation according to any one of claims 1 to 5, characterized in that: The food-grade calcium salt is any one or more of calcium chloride, calcium lactate and calcium carbonate.

7. The method for preparing the enteric-coated sustained-release gel particles for oral beauty and anti-oxidation according to any one of claims 1 to 6, characterized in that: The steps include: (1) Mix collagen peptide, vitamin C and purified water at a material-water ratio of 1:3 g / ml, heat in a water bath at 85°C to dissolve the collagen peptide solution, and keep warm for later use; (2) Mix sodium alginate and purified water at a material-water ratio of 1:14.8 g / ml, heat in a water bath at 85°C to dissolve the sodium alginate solution, and keep warm for later use; (3) Mix the thickener, emulsifier and purified water at a material-water ratio of 1:14.6 g / ml, heat in a water bath at 85°C to dissolve the composite adhesive solution, and keep it warm for later use; (4) Mix food-grade calcium salt with purified water at a material-water ratio of 1:1 g / ml to obtain a calcium suspension for later use; (5) Mix the sodium alginate solution of step (2) and the composite adhesive solution of step (3), heat the mixture in a water bath at 85° C. to obtain a sodium alginate composite adhesive solution, and keep the mixture warm for later use; The sodium alginate composite gel solution in step (5) and the collagen peptide solution in step (1) are mixed and added to the pill dropping machine. The pill dropping machine is started and the nozzle speed is adjusted so that the nozzle sprays out gel particles and the gel particles fall into the calcium suspension in step (4) which has been cooled to 8° C. After being fully formed, they are soaked for 3-5 minutes, taken out and rinsed with pure water for 3-5 times to obtain enteric-coated sustained-release gel particles.

8. Use of the gel particles according to any one of claims 1 to 6 in improving the absorption of collagen peptides in the intestine.

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