Deodorizing and degerming agent special for pets and preparation method of deodorizing and degerming agent
By using natural ingredients such as persimmon tannins, catechins, allicin in pet disinfectants, combined with the synergistic effect of composite double-stranded quaternary ammonium salt and nanosol, the shortcomings of existing disinfectants in terms of disinfection effect, safety and convenience are solved, and an efficient, safe and non-toxic anti-odorant and sterilization effect is achieved.
Patent Information
- Application Number
- CN202510377540.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-28
- Publication Date
- 2025-04-25
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Existing pet disinfectants have shortcomings in disinfection effect, safety, pet health and friendliness and ease of use, and may leave harmful residues, causing secondary pollution.
Special deodorant anti-odorant agent for pets using persimmon tannins, catechins, allicin, composite double-stranded quaternary ammonium salts, nanosols and sustained-release nanoparticles. Through ultrasonic treatment, dynamic mixing and sustained-release mechanism and other technical means, the finished product is colorless, odorless, safe and non-toxic, and convenient to use.
It has achieved a special deodorization and sterilization agent for pets with a powerful disinfection effect at extremely low concentrations. It can effectively kill a variety of microorganisms, and is non-toxic and non-irritating to the human body and pets. It has stable use and reduces the risk of secondary pollution.
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Figure CN119868381A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of pet care products, in particular to a pet-specific deodorizing and degerming agent and a preparation method thereof. Background Art
[0002] As pets play an increasingly important role in human families, the health and environmental safety of pets have gradually attracted widespread attention. Pets are not only emotional sustenance, but also bring countless joy and happiness to the family. However, pets may become the source of transmission of a variety of zoonotic diseases, posing a direct or indirect threat to human health. For example, the contamination of pet feces, direct contact between pets and humans, and pathogens carried by pets themselves may lead to the spread of diseases such as rabies, tuberculosis, and toxoplasmosis. In addition, pets may also cause health problems such as allergic dermatitis and allergic asthma, and even cause cross-infection of pet epidemic diseases.
[0003] Therefore, in order to reduce the health risks brought by pets, pet deodorization and sterilization are particularly important. Existing pet disinfection products mainly achieve this goal through disinfectants, but the use of these disinfectants also faces many challenges. First of all, most of the existing disinfectants need to be diluted before use, which leads to the problem of unstable disinfection effect due to different dilution multiples. Improper dilution may result in insufficient disinfection effect, or excessive concentration of disinfectant, and even cause discomfort to pets. Secondly, some disinfectants, especially alcohol disinfectants, will bring flammable and explosive safety hazards when volatilizing in the air, especially in poorly ventilated environments, which may cause fires and other accidents. Furthermore, existing disinfectants may leave harmful residues after use, causing secondary pollution, and long-term use may have adverse effects on pets, humans and the environment.
[0004] Especially for pets, the use of existing disinfectants often requires great caution. For example, dogs naturally like to sniff everywhere. Once they come into contact with disinfectants, the irritating ingredients may be inhaled, causing chronic health problems. Cats have the habit of licking their fur. If disinfectants are accidentally rubbed on their bodies, they may ingest harmful ingredients through licking, which will affect their health. Therefore, the development of pet-specific disinfectants needs to have higher safety to ensure that they are harmless to pets and the environment.
[0005] These problems of the prior art need to be solved urgently. In order to better serve pet families, it is urgent to provide an efficient, safe, non-toxic, non-irritating and easy-to-use disinfectant. Based on this, the present invention proposes a pet-specific deodorizing and degerming agent and a preparation method thereof. Summary of the invention
[0006] In view of the deficiencies in the prior art, the present invention provides a pet-specific deodorizing and debacterizing agent and a preparation method thereof, which solves the problems of deficiencies in the existing pet disinfectants in terms of disinfection effect, safety, pet health friendliness and ease of use.
[0007] To achieve the above objectives, the present invention is implemented through the following technical scheme: a deodorizing and sterilizing agent for pets, comprising the following components by weight percentage: 1.0%-1.5% persimmon tannin, 0.4%-0.6% catechin, 0.02%-0.05% allicin, 0.3%-0.5% disodium ethylenediaminetetraacetic acid, 0.02%-0.08% sodium lauryl sulfate, 0.8%-1.2% propylene glycol, 0.2%-0.3% ethanol, 96%-97% water, 0.1%-0.3% composite double-chain quaternary ammonium salt, 0.01%-0.05% nanosol, 0.05%-0.1% sustained-release nanoparticles, 0.05%-0.1% polymer synergist, 0.05%-0.2%, 0.05%-0.1% natural plant extract, and 0.01%-0.05% natural flavor component.
[0008] Preferably, the nano sol is nano silver sol or nano titanium dioxide sol.
[0009] Preferably, the composite double-chain quaternary ammonium salt is benzalkonium chloride.
[0010] Preferably, the sustained-release nanoparticles are prepared from chitosan and nano-silver or nano-titanium dioxide, and have a particle size of 50-200 nm. The loading amount of the nano-silver or nano-titanium dioxide is 0.5%-5% of the total weight of the particles.
[0011] Preferably, the natural plant extract is rosemary extract or tea polyphenols, the polymer enhancer is sodium polyacrylate or polyethylene glycol, and the natural flavor component is coconut essence or lavender essential oil.
[0012] A method for preparing a pet-specific deodorizing and sterilizing agent comprises the following steps: S1. Solution basic mixing and activation: persimmon tannin, catechin, allicin, propylene glycol, ethanol and water are mixed, and ultrasonic treatment is performed for 5-10 minutes, followed by stirring until uniform, and then the mixture is cooled to room temperature and stirred for 20-30 minutes; S2. Optimization of surfactant and chelating agent dissolution: Sodium dodecyl sulfate and disodium ethylenediaminetetraacetate were dissolved in two parts of water respectively, treated with a high shear disperser for 3-5 minutes respectively, and then slowly added to the solution of step S1, with the stirring speed controlled at 300-400 rpm, and stirring continued for 8-10 minutes; S3, dynamic mixing of the composite double-chain quaternary ammonium salt: benzalkonium chloride solution is added dropwise to the solution obtained in step S2 by titration at a speed of 0.05-0.1 mL / min, while a magnetic stirrer is used to keep the solution rotating, the stirring speed is controlled at 350-450 rpm, and low-speed ultrasonic treatment is used for 8-10 min; S4, stabilization compounding of nanosol: dilute the nanosol to 50% of the target concentration, let it stand for 25-30 minutes under stirring, then slowly inject it into the solution obtained in step S3, and use a high-speed disperser to process for 5-10 minutes to make the nanoparticles evenly distributed; S5, adding sustained-release particles and natural plant extracts: dissolve the sustained-release nanoparticles and natural plant extracts in a small amount of water, add them to the solution obtained in step S4 with normal stirring, and stir for 10-15 minutes to ensure uniformity; S6. Precise control of pH value: measure the current pH value of the solution. If it is acidic, slowly add dilute sodium hydroxide solution. If it is alkaline, slowly add dilute phosphoric acid solution. Use a real-time online pH monitoring system. Stir for 5-10 minutes after each adjustment until the pH value stabilizes at 7.0±0.1, and add sodium citrate as a buffer. S7, multi-stage filtration and defoaming treatment: the solution is sequentially filtered through 0.45 μm and 0.22 μm filter membranes for multi-stage filtration, and after filtration, it is placed in a vacuum tank and kept for 10-15 minutes to remove bubbles to obtain a final transparent solution; S8. Filling and aseptic packaging: Fill the solution into a spray bottle or lotion bottle that has been sterilized at high temperature and high pressure under a 100,000-class aseptic environment, and seal the bottle mouth with hot-melt sealing technology.
[0013] Preferably, in the step S1, the ultrasonic treatment temperature is 30-35° C., the stirring speed is 300-500 rpm, the power is 100-200 W, and the frequency is 20-25 kHz.
[0014] Preferably, the shear speed of the high shear disperser in step S2 is 6000-8000 rpm, and the power of the low-speed ultrasonic treatment in step S3 is 80-120 W, and the frequency is 15-20 kHz.
[0015] Preferably, the shearing speed of the high-speed disperser in step S4 is 4000-6000 rpm, and the normal speed stirring speed in step S5 is 300-400 rpm.
[0016] The present invention provides a pet-specific deodorizing and sterilizing agent and a preparation method thereof. It has the following beneficial effects: 1. The pet-specific deodorizing and sterilizing agent of the present invention adopts ingredients such as composite double-chain quaternary ammonium salts (such as benzalkonium chloride), has excellent bactericidal effect, can exert a powerful disinfection effect at extremely low concentrations, and can effectively kill a variety of microorganisms, including bacteria, fungi and some viruses. Compared with traditional disinfectants, the disinfectant of the present invention can maintain excellent bactericidal performance without the need for high concentrations, which not only saves the amount of disinfectant used, but also reduces the cost of use.
[0017] 2. The ingredients used in the present invention are non-toxic and non-irritating to humans and pets, especially very friendly to the skin and mucous membranes. By optimizing the ingredients and formula, the disinfectant can ensure that it will not cause skin allergies or other discomfort reactions in daily use. The disinfectant is colorless and odorless, will not pollute or stain objects, and will not corrode or bleach the hair or skin of pets, thereby avoiding the harm and adverse reactions that may be caused by traditional disinfectants.
[0018] 3. The disinfectant of the present invention has good solubility and surface activity, can be easily mixed with water-soluble substances and quickly and evenly distributed, ensuring that the disinfection process is convenient and rapid. At the same time, the solution is stable in nature, has strong light resistance, heat resistance and storage resistance, and is suitable for long-term storage. Its low volatility also effectively reduces the risk of secondary pollution, especially avoids the potential safety hazards of volatile alcohol disinfectants, and provides a more efficient and safe use experience. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION
[0020] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0021] Please refer to the attached Figure 1The embodiment of the present invention provides a pet-specific deodorizing and sterilizing agent, which includes the following components by weight percentage: 1.0%-1.5% persimmon tannin, 0.4%-0.6% catechin, 0.02%-0.05% allicin, 0.3%-0.5% disodium ethylenediaminetetraacetic acid, 0.02%-0.08% sodium lauryl sulfate, 0.8%-1.2% propylene glycol, 0.2%-0.3% ethanol, 96%-97% water, 0.1%-0.3% composite double-chain quaternary ammonium salt, 0.01%-0.05% nanosol, 0.05%-0.1% sustained-release nanoparticles, 0.05%-0.1% polymer synergist, 0.05%-0.2%, 0.05%-0.1% natural plant extract, and 0.01%-0.05% natural fragrance component.
[0022] The nano sol is nano silver sol or nano titanium dioxide sol.
[0023] The complex double-chain quaternary ammonium salt is benzalkonium chloride.
[0024] The sustained-release nanoparticles are prepared from chitosan and nano-silver or nano-titanium dioxide, and have a particle size of 50-200 nm. The loading amount of the nano-silver or nano-titanium dioxide is 0.5%-5% of the total weight of the particles.
[0025] The natural plant extract is rosemary extract or tea polyphenols, the polymer enhancer is sodium polyacrylate or polyethylene glycol, and the natural flavor component is coconut essence or lavender essential oil.
[0026] Specifically, persimmon tannin is a natural plant polyphenol with excellent antibacterial, antioxidant and deodorizing properties. Its antibacterial mechanism is mainly through binding to bacterial cell membranes, destroying the integrity of the cell membranes, and causing bacterial death; at the same time, the phenolic hydroxyl structure in its molecules can neutralize odor molecules, thereby achieving a deodorizing effect. Persimmon tannin can also work synergistically with other bactericidal ingredients to improve the overall disinfection effect.
[0027] Catechins are the main components of tea polyphenols. They can inhibit the enzyme activity required for bacterial metabolism, block the growth and reproduction of bacteria, and have good broad-spectrum antibacterial effects. In addition, catechins can scavenge oxygen free radicals and enhance the antioxidant capacity of the formula, thereby extending the shelf life of the product.
[0028] Allicin has a strong bactericidal and antifungal effect. Its mechanism is to destroy the cell wall and cell membrane of microorganisms, causing the cell contents to leak out, thereby inhibiting or killing pathogens. In addition, the volatile components of allicin can neutralize and decompose pet odor molecules, further improving the deodorizing performance of the product.
[0029] Disodium EDTA acts as a chelating agent by binding to metal ions (such as Ca 2+ Mg 2+) forms a stable chelate, reducing the interference of metal ions on the disinfectant ingredients, thereby improving the stability of the formula. At the same time, disodium EDTA can also enhance the bactericidal effect by destroying the metal ion structure in the bacterial cell wall.
[0030] Sodium dodecyl sulfate is a surfactant that can reduce the surface tension of the solution, thereby increasing the spreadability and permeability of the disinfectant on the surface. Its bactericidal mechanism is to act on the lipid bilayer on the bacterial membrane, destroy the integrity of the membrane, cause the loss of cell contents, and ultimately lead to bacterial death.
[0031] As a solvent, propylene glycol can improve the solubility and dispersibility of other active ingredients, and has a certain moisturizing effect, preventing the solution from evaporating during storage. Ethanol directly destroys the bacterial cell membrane through protein denaturation and lipid dissolution, further enhancing the bactericidal effect.
[0032] Benzalkonium chloride is a cationic surfactant that kills microorganisms by interacting with the cell membranes of bacteria and viruses, disrupting the structural integrity of the membranes. Its low toxicity and high efficacy make it an ideal choice for pet-friendly disinfectants.
[0033] Nanosilver releases silver ions to bind to the DNA and proteins of microorganisms, inhibiting their replication and metabolism, thereby killing bacteria and viruses; nano-titanium dioxide produces active oxygen species through photocatalysis, thereby oxidizing and decomposing pathogens. At the same time, the high surface area of nanoparticles makes them have the characteristics of long-term sustained release.
[0034] Chitosan-loaded nanoparticles can control the slow release of bactericidal ingredients (such as silver ions or titanium dioxide), ensuring the persistence of the bactericidal effect while reducing the irritation of high-concentration bactericides to pets' skin. Chitosan can also further enhance the antibacterial and moisturizing effects.
[0035] Natural plant extracts such as rosemary extract or tea polyphenols can not only enhance the antioxidant properties of the product, but also play an auxiliary bactericidal and antibacterial role, while ensuring the naturalness and safety of the product ingredients.
[0036] The addition of sodium polyacrylate or polyethylene glycol can enhance the adhesion of the disinfectant to the pet's skin or hair, prolong the action time, and thus improve the efficiency of the disinfectant.
[0037] Ingredients such as coconut essence or lavender essential oil can both neutralize the odor of chemical ingredients and provide a pet-friendly, fresh scent to the product, reducing pets' resistance to the product use process.
[0038] A method for preparing a pet-specific deodorizing and sterilizing agent comprises the following steps: S1. Solution basic mixing and activation: persimmon tannin, catechin, allicin, propylene glycol, ethanol and water are mixed, and ultrasonic treatment is performed for 5-10 minutes, followed by stirring until uniform, and then the mixture is cooled to room temperature and stirred for 20-30 minutes; S2. Optimization of surfactant and chelating agent dissolution: Sodium dodecyl sulfate and disodium ethylenediaminetetraacetate were dissolved in two parts of water respectively, treated with a high shear disperser for 3-5 minutes respectively, and then slowly added to the solution of step S1, with the stirring speed controlled at 300-400 rpm, and stirring continued for 8-10 minutes; S3, dynamic mixing of the composite double-chain quaternary ammonium salt: benzalkonium chloride solution is added dropwise to the solution obtained in step S2 by titration at a speed of 0.05-0.1 mL / min, while a magnetic stirrer is used to keep the solution rotating, the stirring speed is controlled at 350-450 rpm, and low-speed ultrasonic treatment is used for 8-10 min; S4, stabilization compounding of nanosol: dilute the nanosol to 50% of the target concentration, let it stand for 25-30 minutes under stirring, then slowly inject it into the solution obtained in step S3, and use a high-speed disperser to process for 5-10 minutes to make the nanoparticles evenly distributed; S5, adding sustained-release particles and natural plant extracts: dissolve the sustained-release nanoparticles and natural plant extracts in a small amount of water, add them to the solution obtained in step S4 with normal stirring, and stir for 10-15 minutes to ensure uniformity; S6. Precise control of pH value: measure the current pH value of the solution. If it is acidic, slowly add dilute sodium hydroxide solution. If it is alkaline, slowly add dilute phosphoric acid solution. Use a real-time online pH monitoring system. Stir for 5-10 minutes after each adjustment until the pH value stabilizes at 7.0±0.1, and add sodium citrate as a buffer. S7, multi-stage filtration and defoaming treatment: the solution is sequentially filtered through 0.45 μm and 0.22 μm filter membranes for multi-stage filtration, and after filtration, it is placed in a vacuum tank and kept for 10-15 minutes to remove bubbles to obtain a final transparent solution; S8. Filling and aseptic packaging: Fill the solution into a spray bottle or lotion bottle that has been sterilized at high temperature and high pressure under a 100,000-class aseptic environment, and seal the bottle mouth with hot-melt sealing technology.
[0039] In step S1, the ultrasonic treatment temperature is 30-35°C, the stirring speed is 300-500rpm, the power is 100-200W, and the frequency is 20-25kHz.
[0040] The shearing speed of the high shear disperser in step S2 is 6000-8000 rpm, and the power of the low-speed ultrasonic treatment in step S3 is 80-120 W, and the frequency is 15-20 kHz.
[0041] The shearing speed of the high-speed disperser in step S4 is 4000-6000 rpm, and the normal speed stirring speed in step S5 is 300-400 rpm.
[0042] Example 1: Preparation method of pet deodorizing and sterilizing agent Materials weighing: Weigh 1.2g persimmon tannin, 0.5g catechin, 0.03g allicin, 1.0g propylene glycol, and 0.25g ethanol, and add them to 96.5g deionized water. The initial solution is light yellow and has no odor.
[0043] Basic mixing and activation: Place the above solution in an ultrasonic processor, set the power to 150W, the frequency to 22kHz, and the processing time to 8 minutes. After the processing, cool it to room temperature (about 25°C) and continue to stir at 400rpm for 30 minutes until the solution is completely uniform and transparent.
[0044] Addition of surfactant and chelating agent: Dissolve 0.04g sodium dodecyl sulfate in 5g deionized water and process with high shear disperser for 3min at a shear rate of 7000rpm. Dissolve 0.4g disodium ethylenediaminetetraacetate in 5g deionized water and process for 5min. After uniform dispersion, slowly add the two to the above-mentioned basic solution in turn, control the stirring speed at 350rpm, continue stirring for 10min, and the solution is clear and transparent.
[0045] Mixing of composite double-chain quaternary ammonium salt: 0.2g of benzalkonium chloride was prepared into a 10% solution, and it was dripped into the solution at a rate of 0.05mL / min by titration. During the titration, a magnetic stirrer was used to keep the solution rotating at a speed of 400rpm, and ultrasonic waves (power 100W, frequency 18kHz) were used for 10min. The solution gradually turned into a uniform light yellow color.
[0046] Addition of nanosol: Dilute 0.03g of nanosilver sol to 50% of the target concentration, let it stand for 25 minutes, then slowly inject the above solution under stirring (300rpm), and then use a high-speed disperser to shear at a speed of 5000rpm for 6 minutes. Observe that the solution is uniform and has no particles or sediments.
[0047] Sustained-release particles and plant extracts: Weigh 0.07 g of chitosan-loaded nanosilver particles and 0.08 g of rosemary extract and dissolve them in a small amount of deionized water. Stir at 300 rpm and slowly pour the solution into the mixture. Continue stirring for 12 minutes. The solution is transparent and has a fresh smell.
[0048] pH adjustment: The initial pH value of the measured solution is 6.4, and dilute sodium hydroxide solution is added drop by drop to slowly adjust to 7.0. Stir for 8 minutes after each adjustment, and use an online pH monitor to monitor in real time until it stabilizes at 7.0±0.1. At the same time, add 0.2g of sodium citrate as a buffer.
[0049] Filtration and defoaming: The solution was filtered through 0.45μm and 0.22μm filter membranes in turn, and then placed in a vacuum tank (vacuum degree -0.09MPa) for defoaming for 12 minutes. The solution was bubble-free and clear.
[0050] Filling and packaging: Under aseptic conditions, the solution is filled into a high-temperature sterilized spray bottle. The bottle mouth is sealed with hot melt sealing technology. The finished product has no odor and is light yellow.
[0051] Example 2: Preparation of a scented pet sterilizer Material ratio: weigh 1.3g persimmon tannin, 0.5g tea polyphenols, 0.04g allicin, 0.9g propylene glycol, 0.2g ethanol, weigh 0.03g coconut essence, and mix all the components into 94.8g deionized water.
[0052] Basic treatment: The solution is treated with an ultrasonic processor at a power of 200W and a frequency of 20kHz for 10 minutes. After treatment, the solution is cooled and stirred until uniform. The liquid has no suspended particles and is transparent.
[0053] Addition of surfactant and chelating agent: 0.06g sodium dodecyl sulfate and 0.3g disodium ethylenediaminetetraacetate were dissolved in separate water. After high shear treatment, the two solutions were slowly added to the base solution, with the stirring speed controlled at 350rpm for 9 minutes.
[0054] Benzalkonium chloride addition: Benzalkonium chloride solution (concentration 10%) was added to the solution at a rate of 0.08 mL / min by titration, and stirring and ultrasonication (power 120 W) were performed simultaneously for 10 minutes. After completion, the liquid was transparent and uniform.
[0055] Add nano-titanium dioxide sol: dilute 0.05 g of nano-titanium dioxide sol to 50%, slowly add it into the main solution under stirring, and shear it with a high-speed disperser for 6 minutes at a speed of 6000 rpm.
[0056] Sustained-release particles and flavor addition: Add 0.06 g of chitosan-loaded nanoparticles and coconut flavor and stir for 5 min. The solution has a fresh smell and no obvious color change.
[0057] pH control, filtration, defoaming: adjust the solution pH to 7.0, add sodium citrate as a buffer, and then perform vacuum defoaming for 10 minutes after double filtration.
[0058] Packaging: The solution is dispensed into sterilized washing liquid bottles and aseptically packaged.
[0059] Example 3: Pet disinfectant with enhanced stability The raw materials were weighed: 1.0 g persimmon tannin, 0.6 g catechin, 0.02 g allicin, 1.1 g propylene glycol, 0.1 g sodium polyacrylate, 0.07 g rosemary extract, and 95.8 g water.
[0060] Basic mixing: Treat the above solution with ultrasound (power 150 W, frequency 22 kHz) for 6 min, then cool and stir until uniform.
[0061] High shear dispersion: Add 0.03 g sodium dodecyl sulfate and 0.4 g disodium ethylenediaminetetraacetate, treat with a shear machine for 5 minutes, and then add slowly.
[0062] Nanosol and benzalkonium chloride: Add diluted nanosilver sol and benzalkonium chloride solution, use dynamic mixing technology, stir at 400 rpm for 12 minutes.
[0063] Stabilization treatment: Add 0.07 g of sustained-release granules and rosemary extract, stir for 10 min. Adjust the pH to 7.0 and add sodium citrate.
[0064] Packaging: After filtering, vacuum remove bubbles and fill into sterile spray bottles.
[0065] Comparative Example 1: No Nanosol Added process: Weigh materials: Weigh 1.2 g persimmon tannin, 0.5 g catechin, 0.03 g allicin, 1.0 g propylene glycol, and 0.25 g ethanol, add to 97.0 g deionized water, and mix well.
[0066] Basic treatment: Place the above solution in an ultrasonic processor with a power of 150 W, a frequency of 22 kHz, and a treatment time of 8 min. After cooling to room temperature, stir for 30 min.
[0067] Addition of surfactant and chelating agent: 0.04 g sodium dodecyl sulfate and 0.4 g disodium ethylenediaminetetraacetate were dissolved in separate water, and then gradually added dropwise after being treated by a high shear disperser, and stirred at 350 rpm for 10 minutes.
[0068] Mixing of composite double-chain quaternary ammonium salts: 0.2 g of benzalkonium chloride was prepared into a 10% solution, added to the base solution in a titration manner (0.05 mL / min), stirred at 400 rpm, and ultrasonically treated for 10 min.
[0069] Add the sustained-release granules and natural plant extracts: add 0.07 g of sustained-release granules and 0.08 g of rosemary extract and stir well.
[0070] pH adjustment, filtration and packaging: Adjust the pH value to 7.0, filter with 0.45μm and 0.22μm filter membranes, then fill and seal aseptically.
[0071] Comparative Example 2: No sustained-release nanoparticles were used process: Weigh materials: Weigh 1.3 g persimmon tannin, 0.5 g tea polyphenols, 0.04 g allicin, 0.9 g propylene glycol, 0.2 g ethanol, and 0.03 g coconut essence, and add to 95.03 g deionized water.
[0072] Basic mixing: The solution was ultrasonically treated at a power of 200 W, a frequency of 20 kHz, and a time of 10 min. After cooling to room temperature, the solution was stirred evenly.
[0073] Addition of surfactant and chelating agent: Dissolve 0.06 g sodium dodecyl sulfate and 0.3 g disodium ethylenediaminetetraacetate separately, add to the base solution after high shear dispersion treatment, and stir for 9 minutes.
[0074] Benzalkonium chloride and nano-sol were added: benzalkonium chloride solution was added at 0.08 mL / min, stirred at 400 rpm, and treated with low-speed ultrasonic waves for 10 min; then, nano-silver sol diluted to 50% was added and dispersed at high speed for 6 min.
[0075] Plant extract and flavor addition: Add rosemary extract and coconut essence and stir for 5 minutes.
[0076] pH adjustment and packaging: After adjusting the pH to 7.0, filter and remove bubbles, and complete the filling.
[0077] Comparative Example 3: No composite double-chain quaternary ammonium salt (benzalkonium chloride) process: Weigh materials: Weigh 1.0 g persimmon tannin, 0.6 g catechin, 0.02 g allicin, 1.1 g propylene glycol, 0.1 g sodium polyacrylate, and 0.07 g rosemary extract, and add 96.6 g deionized water.
[0078] Basic mixing: The solution was treated with ultrasound (power 150 W, frequency 22 kHz) for 6 min, then cooled and stirred evenly.
[0079] High shear dispersion treatment: 0.03 g of sodium dodecyl sulfate and 0.4 g of disodium ethylenediaminetetraacetate were dissolved separately, and then added to the solution after being treated by a high shear disperser.
[0080] Add nano sol: dilute nano titanium dioxide sol to 50%, use high-speed dispersion technology for shearing, time 10 minutes, speed 6000rpm.
[0081] Plant extracts and other additives: 0.07 g of rosemary extract and chitosan-loaded particles were added and stirred evenly.
[0082] pH adjustment and filtration packaging: After adjusting the pH to 7.0, filter, remove bubbles, fill and seal.
[0083] Comparative Example 4: Not adjusted to neutral pH process: Materials were weighed: 1.2 g persimmon tannin, 0.5 g catechin, 0.03 g allicin, 1.0 g propylene glycol, 0.25 g ethanol, 0.03 g nano-silver sol, and 96.9 g deionized water were added.
[0084] Basic treatment: The solution was ultrasonically treated for 8 minutes and then stirred evenly.
[0085] Surfactant and chelating agent are added: sodium dodecyl sulfate and disodium ethylenediaminetetraacetic acid are gradually added to the solution after being dispersed.
[0086] Benzalkonium chloride addition: Benzalkonium chloride solution was added by titration with ultrasonic stirring.
[0087] Omit pH adjustment, filter and package directly: No acid-base adjustment, filter and fill directly.
[0088] Comparative Example 5: No natural plant extracts added process: Weigh the following materials: 1.3 g persimmon tannin, 0.04 g allicin, 0.9 g propylene glycol, 0.2 g ethanol, 0.03 g coconut essence, and add 96.5 g deionized water.
[0089] Basic mixing: The solution was treated with ultrasound for 10 min and then stirred evenly.
[0090] Surfactant and chelating agent: After the surfactant and disodium ethylenediaminetetraacetic acid are dissolved, add them in sequence and stir evenly.
[0091] Nanosol and benzalkonium chloride: Add diluted nanosilver sol and benzalkonium chloride solution, and use dispersion and ultrasonic technology to treat.
[0092] Directly adjust pH to 7.0 and package: Filling is completed after filtering the solution, without adding plant extracts.
[0093] Comparative Example 6: No surfactant (sodium lauryl sulfate) added process: Materials were weighed: 1.2 g persimmon tannin, 0.5 g catechin, 0.03 g allicin, 1.0 g propylene glycol, 0.25 g ethanol, 0.03 g nanosilver sol, 0.2 g benzalkonium chloride, and 96.69 g deionized water were added.
[0094] Ultrasonication and mixing: Ultrasonication for 8 minutes and then stirring until uniform.
[0095] Chelating agent addition: only add disodium EDTA and distribute evenly after stirring.
[0096] The subsequent process is the same: adding nanosol and benzalkonium chloride, adjusting the pH to 7.0, filtering and filling.
[0097] Experiment 1: Sterilization effect test Experimental description: Objective: To test the bactericidal effects of the embodiments and comparative examples on common microorganisms (Escherichia coli, Staphylococcus aureus, Candida albicans) and verify the bactericidal performance of the present invention.
[0098] Experimental steps: Sample preparation: The disinfecting solutions of Examples 1, 2, 3 and Comparative Examples 1, 2, 3 were used.
[0099] Prepare samples to working concentrations (1:10, 1:100, 1:1000 dilutions).
[0100] The control group was untreated bacterial solution (blank control).
[0101] Microbial culture: Standard strains of Escherichia coli, Staphylococcus aureus and Candida albicans were cultured in LB medium and grown at 37°C to the logarithmic growth phase (bacterial concentration of about 10 6 CFU / mL).
[0102] Sterilization treatment: Each bacterial solution was mixed with samples of different concentrations (example and comparative example) at a ratio of 1:10.
[0103] The container was stirred evenly and incubated at 37°C for different time periods (5 min, 10 min, 30 min).
[0104] The reaction was terminated at each time point, and 100 μL of the mixture was diluted 10-fold and inoculated onto LB medium plates.
[0105] Cultivation and counting: The plate was placed in a 37°C incubator and cultured for 24 h.
[0106] The colony count (CFU) of each group of sample plates was recorded and the sterilization rate was calculated.
[0107] Calculate the sterilization rate: sterilization rate (%) = (initial colony count - residual colony count) / initial colony count × 100%.
[0108] Experimental setup: Test samples: Examples 1, 2, 3; Comparative Examples 1, 2, 3.
[0109] Test bacteria: Escherichia coli (E.coli), Staphylococcus aureus (S.aureus), Candida albicans (C.albicans).
[0110] Each group of experiments was repeated 3 times.
[0111] Experimental data: Table 1: Test results of the bactericidal effect of different samples on microorganisms (Experiment 1)
[0112] Experimental summary: The experimental results show that the bactericidal efficiency of Examples 1, 2, and 3 far exceeds that of the comparative example, especially in a short time. After 5 minutes of action, the bactericidal rate of Example 1 against Escherichia coli is close to 99%, and the bactericidal rate against Staphylococcus aureus and Candida albicans is also over 95%. This is mainly due to the synergistic effect of the composite double-chain quaternary ammonium salt and the nanosol. Benzalkonium chloride can quickly destroy the bacterial cell membrane, while nanosilver further interferes with the metabolic system of bacteria by releasing silver ions, thereby achieving a rapid and thorough bactericidal effect. Moreover, the combination of catechins and persimmon tannins also exhibits a significant inhibitory effect on fungi.
[0113] In contrast, due to the lack of nanosol, the bactericidal efficiency of Comparative Example 1 is significantly reduced when the action time is short, especially for Candida albicans, with a bactericidal rate of only about 70%. This shows that although the composite double-chain quaternary ammonium salt can effectively kill bacteria, it lacks the synergistic effect of nanosilver, and its persistence and comprehensiveness are insufficient. As for Comparative Example 3 (without composite double-chain quaternary ammonium salt), its performance is more limited, and it can only achieve a bactericidal rate of 60%-70%. This clearly shows that relying solely on nanosilver cannot meet the actual broad-spectrum bactericidal needs.
[0114] The long-term bactericidal effect also reveals the key role of the slow-release particles. After dilution to 1:100, Examples 2 and 3 still maintained a bactericidal rate of more than 90%, while Comparative Example 2 quickly dropped to less than 80%. The chitosan slow-release system can gradually release the active ingredients and extend the time window for sterilization. This slow-release mechanism, combined with the rapid action of nanosol and composite quaternary ammonium salt, makes the embodiments more suitable for daily pet care and environmental disinfection, far superior to traditional formulations.
[0115] Experiment 2: Deodorization performance test Experimental Description Purpose: To verify the effects of the examples and comparative examples in removing pet odors (such as ammonia and hydrogen sulfide) and to prove the superiority of the present invention in deodorizing ability.
[0116] Experimental steps: Sample preparation: The solutions of Examples 1 and 2 and Comparative Examples 1, 4 and 5 were selected as test samples.
[0117] Each sample was used in the form of stock solution, and the blank control group was untreated (sprayed with deionized water).
[0118] Simulated odor preparation: Use ammonia and hydrogen sulfide gas generator to prepare mixed odor.
[0119] The ammonia concentration was controlled at 100ppm and the hydrogen sulfide concentration was controlled at 50ppm, and they were injected into a 500mL sealed container to ensure uniform gas concentration.
[0120] Test steps: Spray 2 mL of the test sample into the interior of each container.
[0121] The gas concentration sensor is used to record the concentrations of ammonia and hydrogen sulfide in real time at 5 minutes, 15 minutes, and 30 minutes after spraying.
[0122] Each test was repeated 3 times and the average value was recorded.
[0123] Calculation of deodorization efficiency: Deodorization rate (%) = (initial concentration - residual concentration) / initial concentration × 100%.
[0124] Experimental setup: Sample groups: Examples 1 and 2; Comparative Example 1 (without nanosol), Comparative Example 4 (without pH adjustment), and Comparative Example 5 (without plant extract).
[0125] Control group: blank treatment, only odor was injected.
[0126] Experimental data: Table 2: Deodorization efficiency of different samples for ammonia and hydrogen sulfide (Experiment 2)
[0127] Experimental summary: The performance of Examples 1 and 2 fully verified their high deodorization ability. Especially in the early stage of the experiment (within 5 minutes), the deodorization rates of ammonia and hydrogen sulfide reached more than 80% and 75% respectively. This is due to the synergistic effect of persimmon tannin and rosemary extract. Persimmon tannin quickly complexes with ammonia molecules through its polyphenol groups, while the active substances in rosemary extract can adsorb and decompose hydrogen sulfide molecules, significantly improving the overall deodorization efficiency.
[0128] The deodorizing effect of Comparative Example 1 in a short period of time is far behind, and it can only remove about 50% of ammonia and 40% of hydrogen sulfide. The reason is the lack of catalytic and decomposition effect of nanosol. The nanosilver or nanotitanium dioxide sol in the composite formula decomposes harmful gases through surface catalytic reaction, making the embodiment more efficient and durable in the deodorizing process. Comparative Example 4 (without pH adjustment) is acidic, which not only fails to stabilize the active ingredients, but also may greatly weaken the effect of the plant extract.
[0129] It is worth noting that under long-term action (30 minutes), the deodorization efficiency of Example 1 remains at about 90%, while that of Comparative Example 5 drops rapidly. This shows that the plant extract can not only play a short-term deodorization role, but also protect other active ingredients through antioxidant properties, so that they can continue to act on malodor molecules. The multi-level synergistic mechanism of the embodiment not only improves the short-term efficiency, but also achieves a longer-lasting deodorization effect.
[0130] Experiment 3: Safety test (effects on pet skin and environment) Experimental description: Purpose: To evaluate whether the present invention is friendly to pet skin and the environment by testing the differences in irritation and safety between the examples and the comparative examples. The focus is on comparing the optimized formulation in the examples with the comparative examples where pH is not adjusted, stabilizers or other key ingredients are missing.
[0131] Experimental steps: Experimental animal preparation: Five healthy adult dogs and five healthy adult cats were selected and divided into an embodiment group and a comparative example group. A 5 cm×5 cm hairless area was selected on the back of each animal as the test site.
[0132] Each test area was divided into the following treatment groups: Examples 1 and 3; Comparative Examples 4 (without pH adjustment) and 5 (without plant extract); and blank control (sprayed with deionized water).
[0133] Sample smear: Apply 0.2 mL of sample evenly to each test area and massage gently to allow it to fully contact the skin.
[0134] Allow to dry naturally after application without additional cleanup.
[0135] Skin reaction observation: Observe and record skin conditions daily, including external manifestations such as redness, swelling, itching, dryness, and allergic reactions.
[0136] Use a skin resistance meter to measure the resistance value of the test area daily (to reflect the integrity of the skin barrier) and record the data changes for 7 days.
[0137] Inflammatory marker detection: On the 7th day, skin tissue samples were collected from the test site, and inflammatory markers (IL-6, TNF-α) were detected after total protein extraction. The concentration of inflammatory factors was determined using ELISA kits, and the degree of inflammation induction of different samples was compared.
[0138] Experimental setup: Sample groups: Examples 1 and 3; Comparative Examples 4 and 5; Blank control. Each treatment group contains 5 animal samples.
[0139] Experimental data: Table 3: Safety test data of different samples on pet skin (Experiment 3)
[0140] Experimental summary: The experiment shows that Examples 1 and 3 are extremely safe for pet skin. After 7 consecutive days of use, there was no obvious redness, swelling or itching in the test area of the experimental animals, and the skin resistance value was stabilized at 1600-1700Ω, which was almost the same as the blank control group. This performance is due to the neutral pH design in the composite formula and the anti-inflammatory properties of the plant extract. The antioxidants in the rosemary extract can not only relieve the irritation of chemical ingredients, but also maintain the integrity of the skin barrier and prevent the resistance value from decreasing.
[0141] The results of Comparative Example 4 are worrying. The solution without pH adjustment caused skin redness scores as high as 4-5 points, and inflammatory factors such as IL-6 and TNF-α increased significantly. This shows that the acidic solution caused significant damage to the pet's skin barrier, making the skin more vulnerable to external stimuli. In contrast, although Comparative Example 5 has no pH problem, due to the lack of plant extracts, its irritation is also significantly higher than that of the Example Group, and long-term use may still pose a safety hazard.
[0142] The data also reveal the importance of chitosan slow-release particles. The slow-release system of the embodiment group can reduce the instantaneous release of active ingredients, thereby reducing the chemical load on the skin, while ensuring a long-lasting bactericidal effect. Comparative Examples 2 and 3 lack this design, and their rapidly released chemical components may further aggravate the discomfort of the skin. This meticulous optimization design makes the embodiments more in line with practical application needs.
[0143] Experiment 4: Stability test Experimental description: Purpose: To test the stability of the examples and comparative examples under different storage conditions (light and high temperature) and verify the superior performance of the present invention during storage.
[0144] Experimental steps: Sample preparation: The solutions of Examples 2 and 3 and Comparative Examples 2 (without sustained-release particles) and 3 (without composite double-chain quaternary ammonium salt) were used.
[0145] Each group of samples was packaged into 50 mL transparent glass bottles (under light conditions) and brown glass bottles (under high temperature and dark conditions).
[0146] Storage condition settings: Light conditions: simulate sunlight exposure in a constant temperature box (5000 lux light intensity), the temperature is maintained at 25°C, and the storage time is 30 days.
[0147] High temperature conditions: set to 40°C in a light-proof environment, and the storage time is also 30 days.
[0148] Test indicators: Appearance changes: Observe the color and transparency of the samples every 5 days and record the precipitation.
[0149] pH value: Use a pH meter to measure the pH value of the sample, sampling 10 mL each time.
[0150] Active ingredient content: High performance liquid chromatography (HPLC) was used to determine the concentration changes of benzalkonium chloride and nanosilver sol in the samples.
[0151] Bactericidal effect: Take samples every 10 days to test the bactericidal rate of 1:10 dilution against Escherichia coli. Refer to Experiment 1 for the method.
[0152] Experimental setup: Test samples: Examples 2 and 3; Comparative Examples 2 (without sustained-release particles) and 3 (without composite double-chain quaternary ammonium salt); blank control (deionized water).
[0153] Test interval: Check appearance and pH value every 5 days, and check ingredient content and bactericidal effect every 10 days.
[0154] Experimental data: Table 4: Stability test results of different samples under light and high temperature conditions (Experiment 4)
[0155] Experimental summary: The experimental results show that Examples 2 and 3 maintain excellent stability under light and high temperature conditions. After 30 days of storage, the pH value remained stable at 6.7-7.0, with no obvious precipitation or stratification in appearance, and the concentration of active ingredients in benzalkonium chloride and nanosol decreased by less than 10%. This excellent performance is due to the multiple stabilization mechanisms in the formula. EDTA-2Na chelates metal ions to prevent trace impurities in the solution from causing precipitation or decomposition. The sustained-release particles further slow down the degradation of the active substances by controlling the release rate of the ingredients.
[0156] The results of Comparative Example 2 highlight the importance of sustained-release design. Under light and high-temperature storage conditions, the concentration of benzalkonium chloride decreased by nearly 40%, and the precipitation increased significantly. This shows that the active ingredient is difficult to remain stable under external stimuli in the formula lacking sustained-release particles. The high-temperature test performance of Comparative Example 3 was particularly poor, with obvious stratification and turbidity after 30 days, and the bactericidal effect dropped significantly to 50%. The lack of composite double-chain quaternary ammonium salts obviously destroyed the uniformity of the solution, resulting in the inability of the active ingredient to exist stably.
[0157] The experiment also revealed the key role of plant extracts in the protection system. The rosemary extract and tea polyphenols in the example group inhibited the degradation of active ingredients caused by light and high temperature through antioxidant effects, allowing the product to maintain high efficiency under harsh conditions. This stability not only extends the storage period of the product, but also ensures its continued effectiveness in actual use.
[0158] Experiment 5: Durability Test Experimental description: Purpose: To verify the bactericidal and deodorizing effects of the present invention under long-term action, compare with the comparative examples, evaluate the long-term effects of the examples, and highlight the advantages of slow-release particles and synergistic effects.
[0159] Experimental steps: Test sample preparation: The sample groups include: Examples 1 and 2, and Comparative Examples 1 (without nanosol) and 5 (without plant extract).
[0160] Blank control group: sprayed with deionized water.
[0161] All samples used stock solutions to simulate actual usage conditions.
[0162] Experimental environment preparation: A stainless steel plate (10 cm × 10 cm) was selected and the surface was cleaned and sterilized.
[0163] Spray 2 mL of sample on each plate and let it dry naturally to ensure that the liquid is evenly distributed.
[0164] Bactericidal effect test: 100 μL of Escherichia coli solution (concentration 10) was added to the sample surface every 2 h, 6 h, and 12 h. 6 CFU / mL).
[0165] After standing for 15 minutes, smear the surface of the plate with a sterile cotton swab and transfer the bacterial solution to the LB medium plate.
[0166] The plates were cultured at 37°C, and the colony numbers (CFU) were counted after 24 h to calculate the bactericidal rate.
[0167] Deodorizing effect test: The plate was placed in a 500 mL sealed container and injected with a mixed odor of ammonia and hydrogen sulfide (initial concentration: NH3 100 ppm, H2S 50 ppm).
[0168] Use a gas sensor to detect the gas concentration in the container every 2h, 6h, and 12h, and calculate the deodorization rate.
[0169] Experimental setup: Sample groups: Examples 1 and 2; Comparative Examples 1 and 5; Blank control.
[0170] Each group of experiments was repeated 3 times and the average value was taken.
[0171] Experimental data: Table 5: Test results of the bactericidal and deodorizing effects of different samples (Experiment 5)
[0172] Experimental summary: Example 1 performed particularly well in long-term testing. After 12 hours, its bactericidal rate was still maintained at 91.8%, and the deodorization rates of ammonia and hydrogen sulfide were 66.8% and 81.1%, respectively. This durability is mainly due to the design of the chitosan sustained-release particles. The sustained-release particles can gradually release the active ingredients to ensure that the bactericidal and deodorizing effects continue to work for a long time. Combined with the rapid bactericidal ability of the composite double-chain quaternary ammonium salt, the multi-level mechanism of the embodiment is obviously more efficient.
[0173] In contrast, the long-term performance of Comparative Examples 1 and 5 showed obvious disadvantages. After 12 hours, the sterilization rate of Comparative Example 1 dropped to less than 62%, while the ammonia deodorization rate was only 47%. This rapid failure shows that it is difficult for traditional formulas to achieve long-term performance when they lack nanosol and sustained release mechanism. Although the short-term effect of Comparative Example 5 is close to that of the embodiment, the lack of protection of plant extracts leads to accelerated attenuation of active ingredients after long-term use.
[0174] The experiment further demonstrated the contribution of plant extracts to long-lasting performance. Persimmon tannin and rosemary extract not only directly participate in chemical reactions, but also extend the effective time of other active ingredients through antioxidant properties. The collaborative design of the embodiment group enables the product to continue to lead in long-term sterilization and deodorization tests, demonstrating its superiority in practical applications.
[0175] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A pet-specific deodorizing and sterilizing agent, characterized in that: The invention comprises the following components by weight percentage: 1.0%-1.5% persimmon tannin, 0.4%-0.6% catechin, 0.02%-0.05% allicin, 0.3%-0.5% disodium ethylenediaminetetraacetic acid, 0.02%-0.08% sodium lauryl sulfate, 0.8%-1.2% propylene glycol, 0.2%-0.3% ethanol, 96%-97% water, 0.1%-0.3% composite double-chain quaternary ammonium salt, 0.01%-0.05% nanosol, 0.05%-0.1% sustained-release nanoparticles, 0.05%-0.2% polymer enhancer, 0.05%-0.2% natural plant extract, and 0.01%-0.05% natural flavor component.
2. A pet-specific deodorizing and sterilizing agent according to claim 1, characterized in that: The nano sol is nano silver sol or nano titanium dioxide sol.
3. A pet-specific deodorizing and sterilizing agent according to claim 1, characterized in that: The composite double-chain quaternary ammonium salt is benzalkonium chloride.
4. A pet-specific deodorizing and sterilizing agent according to claim 1, characterized in that: The sustained-release nanoparticles are prepared from chitosan and nano-silver or nano-titanium dioxide, and have a particle size of 50-200 nm. The loading amount of the nano-silver or nano-titanium dioxide is 0.5%-5% of the total weight of the particles.
5. The pet-specific deodorizing and sterilizing agent according to claim 1, characterized in that: The natural plant extract is rosemary extract or tea polyphenols, the polymer enhancer is sodium polyacrylate or polyethylene glycol, and the natural flavor component is coconut essence or lavender essential oil.
6. A method for preparing a pet-specific deodorizing and sterilizing agent, using a pet-specific deodorizing and sterilizing agent as claimed in any one of claims 1 to 5, characterized in that: The following steps are involved: S1. Solution basic mixing and activation: persimmon tannin, catechin, allicin, propylene glycol, ethanol and water are mixed, and ultrasonic treatment is performed for 5-10 minutes, followed by stirring until uniform, and then the mixture is cooled to room temperature and stirred for 20-30 minutes; S2. Optimization of surfactant and chelating agent dissolution: Sodium dodecyl sulfate and disodium ethylenediaminetetraacetate were dissolved in two parts of water respectively, treated with a high shear disperser for 3-5 minutes respectively, and then slowly added to the solution of step S1, with the stirring speed controlled at 300-400 rpm, and stirring continued for 8-10 minutes; S3, dynamic mixing of the composite double-chain quaternary ammonium salt: benzalkonium chloride solution is added dropwise to the solution obtained in step S2 by titration at a speed of 0.05-0.1 mL / min, while a magnetic stirrer is used to keep the solution rotating, the stirring speed is controlled at 350-450 rpm, and low-speed ultrasonic treatment is used for 8-10 min; S4, stabilization compounding of nanosol: dilute the nanosol to 50% of the target concentration, let it stand for 25-30 minutes under stirring, then slowly inject it into the solution obtained in step S3, and use a high-speed disperser to process for 5-10 minutes to make the nanoparticles evenly distributed; S5, adding sustained-release particles and natural plant extracts: dissolve the sustained-release nanoparticles and natural plant extracts in a small amount of water, add them to the solution obtained in step S4 with normal stirring, and stir for 10-15 minutes to ensure uniformity; S6. Precise control of pH value: measure the current pH value of the solution. If it is acidic, slowly add dilute sodium hydroxide solution. If it is alkaline, slowly add dilute phosphoric acid solution. Use a real-time online pH monitoring system. Stir for 5-10 minutes after each adjustment until the pH value stabilizes at 7.0±0.1, and add sodium citrate as a buffer. S7, multi-stage filtration and defoaming treatment: the solution is sequentially filtered through 0.45 μm and 0.22 μm filter membranes for multi-stage filtration, and after filtration, it is placed in a vacuum tank and kept for 10-15 minutes to remove bubbles to obtain a final transparent solution; S8. Filling and aseptic packaging: Fill the solution into a spray bottle or lotion bottle that has been sterilized at high temperature and high pressure under a 100,000-class aseptic environment, and seal the bottle mouth with hot-melt sealing technology.
7. The method for preparing a pet-specific deodorizing and sterilizing agent according to claim 6, characterized in that: In the step S1, the ultrasonic treatment temperature is 30-35° C., the stirring speed is 300-500 rpm, the power is 100-200 W, and the frequency is 20-25 kHz.
8. The method for preparing a pet-specific deodorizing and sterilizing agent according to claim 6, characterized in that: The shearing speed of the high shear disperser in step S2 is 6000-8000 rpm, and the power of the low-speed ultrasonic treatment in step S3 is 80-120 W, and the frequency is 15-20 kHz.
9. The method for preparing a pet-specific deodorizing and sterilizing agent according to claim 6, characterized in that: The shearing speed of the high-speed disperser in the step S4 is 4000-6000 rpm, and the normal speed stirring speed in the step S5 is 300-400 rpm.
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