Skin care composition and preparation method thereof

Abstract

The invention relates to the technical field of skin care compositions, and discloses a skin care composition and a preparation method thereof.The preparation method of the skin care composition comprises the steps that on the basis of a hydroxyl-carboxyl esterification reaction mechanism, ethylene diamine tetraacetic acid, lauryl alcohol and sorbitol serve as raw materials to prepare sorbitol ethylene diamine tetraacetic acid lauryl alcohol ester; the preparation method comprises the following steps: based on a quaternization reaction mechanism, preparing an amphiphilic monomer by taking sorbitol ethylene diamine tetraacetic acid lauryl ester and 1-chlorododecane as raw materials; the preparation method comprises the following steps: forming a cavity structure with a hydrophobic interior and a hydrophilic exterior through self-assembly by utilizing the amphiphilic characteristic of an amphiphilic monomer, and enabling madecassoside or asiaticoside to enter a cavity, so as to prepare a madecassoside-loaded microsphere or an asiaticoside-loaded microsphere; the madecassoside-loaded microspheres and the asiaticoside-loaded microspheres are used as active ingredients and are mixed with other basic raw materials, and the mixture is emulsified to prepare the skin care composition. The skin care composition prepared by the invention can improve skin dryness, relieve skin redness and make the skin smooth, soft, tender and fine.

Description

Technical Field

[0001] This invention relates to the field of skin care composition technology, and in particular to a skin care composition and its preparation method. Background Technology

[0002] With rising living standards and a growing pursuit of beauty and health, consumers have an increasing demand for skincare products, especially functional skincare products, such as those that are anti-aging, soothe sensitive skin, moisturize, repair the skin barrier, provide antioxidant protection, and reduce wrinkles. At the same time, they are also placing higher demands on the safety, efficacy, and green ingredients in skincare products.

[0003] Natural plant extracts are increasingly favored by people due to their high safety, multiple skin care benefits, and easy absorption by human skin. Therefore, using natural plant extracts as raw materials to make functional skin care products is gradually becoming the mainstream trend.

[0004] Centella asiatica, also known as snow calendula, is the dried whole herb of the Apiaceae family plant Centella asiatica. It has a long history of use and is abundant. Modern pharmacological studies show that Centella asiatica and its extracts have pharmacological effects such as promoting wound healing, antibacterial activity, antioxidant properties, and scar treatment. The main components of Centella asiatica include asiaticoside, asiaticoside, asiaticoside biglycoside, asiatic acid, asiaticoside, and vanillic acid. Among these, asiaticoside and asiaticoside are the main active ingredients. Research has found that asiaticoside and asiaticoside have good antibacterial, antioxidant, skin growth and collagen synthesis-promoting, fine line-smoothing, skin elasticity-maintaining, whitening, and soothing effects. Furthermore, they can achieve anti-inflammatory effects by improving and repairing the skin's barrier function, thus they can be made into products with acne-eliminating effects and skincare products primarily for after-sun repair.

[0005] Currently, asiaticoside is widely used in skincare products such as lotions, creams, and masks. For example, the invention patent with authorization announcement number CN113633565B discloses an oil-controlling and acne-reducing composition and its skincare products, skincare gel, and preparation method. This composition uses tanshinone, sodium guaiacol, and asiaticoside in a specific ratio to prepare the oil-controlling and acne-reducing composition, which can soothe sensitive skin, treat atopic dermatitis, and repair acne scars. However, asiaticoside and asiaticoside are chemically unstable (sensitive to temperature, pH, and light), and easily oxidize, losing their efficacy and significantly affecting their effectiveness in skincare products. Summary of the Invention

[0006] This invention utilizes a newly developed amphiphilic monomer to coat asiaticoside or asiaticoside to prepare asiaticoside-loaded microspheres or asiaticoside-loaded microspheres, which are then further mixed with other basic raw materials and emulsified to prepare a skin care composition. This skin care composition can improve dry skin, reduce redness, and make the skin soft and delicate.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: a skin care composition comprising the following raw materials in parts by weight: 1-10 portions of asiaticoside-loaded microspheres, using an amphiphilic monomer as a carrier, with the mass ratio of the carrier to the active component asiaticoside being 1:(4.0-4.5); 1-10 portions of asiaticoside microspheres, using an amphiphilic monomer as a carrier, with a mass ratio of the carrier to the active component asiaticoside of 1:(4.0-4.5); 2-4 parts olive oil; 3-5 parts isooctyl palmitate; 2-4 parts cetyl alcohol; 0.2-0.4 parts carbomer; 1-3 parts glycerol; 0.2-0.4 parts triethanolamine; 0.3-0.5 parts Tween 60; 1-3 parts of 1,3-propanediol; 0.4-0.5 parts p-hydroxyacetophenone; 0.01-0.1 parts of 1,2-hexanediol; 60-85 parts ultrapure water.

[0008] Furthermore, the preparation method of the skincare composition includes the following steps: Step 1: Mix olive oil, isooctyl palmitate, and cetyl alcohol evenly to obtain component 1; Step 2: Mix carbomer, glycerol, triethanolamine, Tween 60 and 1,3-propanediol evenly to obtain component 2; Step 3: Heat components 1 and 2 separately in a water bath at 60-80℃ and stir until dissolved. Then add component 1 to component 2 and mix well. Reduce the system temperature to 45-55℃, add asiaticoside-loaded microspheres, p-hydroxyacetophenone and 1,2-hexanediol, stir evenly, and finally add ultrapure water to emulsify and prepare the skin care composition.

[0009] Furthermore, the emulsification time is 10-30 minutes.

[0010] Furthermore, the method for preparing the hydroxyasiaticoside-loaded microspheres is as follows: utilizing the amphiphilic properties of amphiphilic monomers, a chamber structure with an internal hydrophobic and external hydrophilic structure is formed through self-assembly, and hydroxyasiaticoside is coated to obtain hydroxyasiaticoside-loaded microspheres.

[0011] Furthermore, the method for preparing the asiaticoside-loaded microspheres is as follows: utilizing the amphiphilic properties of amphiphilic monomers, a chamber structure with an internal hydrophobic and external hydrophilic structure is formed through self-assembly, and asiaticoside enters the chamber to prepare asiaticoside-loaded microspheres.

[0012] Furthermore, the preparation method of the amphiphilic monomer is as follows: Based on the hydroxy-carboxyl esterification mechanism, ethylenediaminetetraacetic acid (EDTA) reacts with lauryl alcohol in a 1:1 molar ratio under the catalysis of protic acid to prepare EDTA lauryl ester. Based on the hydroxy-carboxyl esterification reaction mechanism, ethylenediaminetetraacetic acid lauryl ester and sorbitol react in a molar ratio of 1:3 to prepare sorbitol-based ethylenediaminetetraacetic acid lauryl ester. Based on the quaternization reaction mechanism, sorbitol ethylenediaminetetraacetic acid lauryl ester reacts with 1-chlorododecane in a molar ratio of 1:2 to prepare an amphiphilic monomer.

[0013] Furthermore, the protic acid is either p-toluenesulfonic acid or trifluoromethanesulfonic acid.

[0014] The beneficial effects of this invention are as follows: Based on the hydroxy-carboxyl esterification reaction mechanism, sorbitol-based ethylenediaminetetraacetic acid lauryl ester was prepared using ethylenediaminetetraacetic acid, lauryl alcohol, and sorbitol as raw materials. Based on the quaternization reaction mechanism, amphiphilic monomers were prepared using sorbitol ethylenediaminetetraacetic acid lauryl ester and 1-chlorododecane as raw materials; By utilizing the amphiphilic properties of amphiphilic monomers, a chamber structure with an internal hydrophobic and external hydrophilic structure is formed through self-assembly, and hydroxyasiaticoside or asiaticoside enters the chamber to prepare hydroxyasiaticoside-loaded microspheres or asiaticoside-loaded microspheres. A skincare composition was prepared by mixing and emulsifying hydroxyascorbic acid microspheres and asiatic acid microspheres as active ingredients with other basic raw materials. Experimental results demonstrate that the skincare composition prepared by this invention can improve dry skin, reduce redness, and leave the skin smooth, soft, and delicate. Detailed Implementation

[0015] To improve the stability of asiaticoside and asiaticoside, this invention independently developed an amphiphilic monomer to encapsulate asiaticoside and asiaticoside, thereby improving their stability. At the same time, the long-chain alkyl group in its structure enhances its skin affinity, thus effectively improving the bioavailability of asiaticoside and asiaticoside.

[0016] Example 1: The preparation of amphiphilic monomers includes the following steps: (1) Preparation of ethylenediaminetetraacetic acid lauryl ester. The preparation mechanism is as follows: ethylenediaminetetraacetic acid lauryl ester is prepared by reacting the carboxyl group in ethylenediaminetetraacetic acid with the hydroxyl group in lauryl alcohol. The specific experimental steps are as follows: 1.9g of lauryl alcohol is added to 50mL of propylene glycol and mechanically stirred until uniform to obtain lauryl alcohol solution. 3g of ethylenediaminetetraacetic acid is added to a 250mL three-necked flask and 30mL of sulfuric acid solution with a concentration of 4mol / L is added. The mixture is heated in an oil bath and stirred continuously. When the temperature rises to 100℃, the solid is completely dissolved. The prepared lauryl alcohol solution and 0.08g of catalyst p-toluenesulfonic acid are added. After the addition is complete, the heating is turned on and the temperature is controlled at 110℃. The mixture is stirred for 8h and nitrogen gas is introduced for protection throughout the process. After the reaction is completed, the mixture is cooled to room temperature, the solvent is removed by rotary evaporation, filtered, washed, and vacuum dried at 50℃ for 10h to obtain ethylenediaminetetraacetic acid lauryl ester. (2) Preparation of sorbitol-based ethylenediaminetetraacetic acid lauryl ester. The preparation mechanism is as follows: sorbitol-based ethylenediaminetetraacetic acid lauryl ester is prepared by reacting the carboxyl group in ethylenediaminetetraacetic acid lauryl ester with the hydroxyl group in sorbitol. The specific experimental steps are as follows: 4g of ethylenediaminetetraacetic acid lauryl ester and 4.8g of sorbitol are added to a 250mL three-necked flask equipped with a constant pressure dropping funnel, an electric stirrer and a condenser. Then, 100mL of 40% (v / v) dimethyl sulfoxide aqueous solution is added and mixed. 0.1g of p-toluenesulfonic acid catalyst is added. After the addition is complete, nitrogen gas is introduced and heating is started. After the system temperature reaches 110℃, the reaction is kept at a constant temperature for 6h. The solvent is removed by vacuum distillation, the solid material is separated by filtration, washed and placed in a vacuum drying oven at 60℃ for 8h to prepare sorbitol-based ethylenediaminetetraacetic acid lauryl ester.

[0017] (3) Preparation of amphiphilic monomers. The preparation mechanism is as follows: the amphiphilic monomers are prepared by the quaternization reaction between the tertiary amine group in sorbitol ethylenediaminetetraacetic acid lauryl ester and the chlorine functional group in 1-chlorododecane. The specific experimental steps are as follows: under nitrogen protection, 5g of sorbitol ethylenediaminetetraacetic acid lauryl ester is added to 100mL of acetone and mechanically stirred until uniform. Then, 2.2g of 1-chlorododecane is added. After the addition is complete, the system temperature is raised to 40℃ and stirred for 12h. After cooling to room temperature, the solvent is removed by rotary evaporation. After washing, the mixture is vacuum dried at 60℃ for 5h to prepare the amphiphilic monomers. The chemical structural formula of the amphiphilic monomer is: ; The 1H NMR spectrum of the amphiphilic monomer is characterized as follows: 1 H NMR (DMSO-d6, 400MHz) δ: 0.88-0.90 (m, 9H), 1.25-1.30 (m, 52H), 1.34-1.42 (m, 6H), 1.60-1.66 (m, 6H), 3.52-3.60 (m, 9H), 3.69- 3.77 (m, 6H), 3.86-3.90 (m, 3H), 4.04-4.07 (m, 7H), 4.13-4.15 (t, 2H), 4.26-4.35 (m, 9H), 4.44-4.45 (d, 6H), 4.52-4.55 (m, 11H).

[0018] Example 2: I. Preparation of asiaticoside-loaded microspheres: The preparation mechanism is as follows: asiaticoside is coated onto a chamber-like structure with an internal hydrophobic and external hydrophilic structure generated by an amphiphilic monomer. The specific experimental steps are as follows: 2g of amphiphilic monomer was mixed with 150mL of deionized water and magnetically stirred until homogeneous. 8g of asiaticoside was added while stirring. After the addition was complete, stirring was continued until a homogeneous solution was formed. After standing for 2 hours, the solution was concentrated under reduced pressure and freeze-dried to obtain asiaticoside-loaded microspheres. II. Preparation of asiaticoside-loaded microspheres: The preparation mechanism is as follows: Due to the amphiphilic nature of the amphiphilic monomer, the hydrophilic group of the amphiphilic monomer faces outward and the hydrophobic group faces inward, creating a chamber-like structure with an internal hydrophobic and external hydrophilic structure. Asiaticoside enters the chamber, thus preparing asiaticoside-loaded microspheres. The specific experimental steps are as follows: 2g of the amphiphilic monomer is added to 50mL of ethanol-water solution (ethanol to water volume ratio of 4:1) and stirred evenly. 8g of asiaticoside is added under stirring conditions. After the addition is complete, stirring is continued until a homogeneous solution is formed. The mixed solution is placed in 100mL of deionized water and allowed to stand for 2 hours. After concentration under reduced pressure and freeze-drying, asiaticoside-loaded microspheres are prepared.

[0019] Example 3: A skin care composition A has the following raw material formula as shown in Table 1 below; Table 1. Formulation of Skin Care Composition A The preparation method of skin care composition A includes the following steps: Olive oil, isooctyl palmitate, and cetyl alcohol were mixed evenly to obtain component one. Carbomer 941, glycerol, triethanolamine, Tween 60, and 1,3-propanediol were mixed evenly to obtain component two. Component one and component two were heated in a water bath at 70°C and stirred until dissolved. Component one was then added to component two and mixed evenly. The system temperature was lowered to 50°C, and asiaticoside microspheres, p-hydroxyacetophenone, and 1,2-hexanediol were added and stirred evenly. Finally, ultrapure water was added, and the mixture was emulsified at 800 r / min for 20 min to prepare skin care composition A. Performance testing: I. Sensory and physicochemical evaluation: The appearance, pH, heat resistance, cold resistance and centrifugal stability of the skin care composition A prepared in this invention were evaluated according to GB / T 29665-2013 "Skin Care Lotions". II. Evaluation of Hygiene Indicators: Using the skin care composition A prepared in this invention as a sample, the total number of colonies was evaluated using lecithin Tween 80 nutrient agar medium. 1 mL of test solution diluted to 1:10 and 1:100 (sample: distilled water) was added to the petri dishes, and then 15 mL of sterilized lecithin Tween 80 nutrient agar medium was injected. The mixture was stirred well. Two groups of test solutions were prepared for each dilution. Sterilized petri dishes without test solution were prepared using the same method as blank control groups. The petri dishes were placed in an incubator at (36±1)℃ and incubated for (48±2) h. The total number of colonies on the culture medium was observed after 48 h. The test results are shown in Table 2 below; Table 2 Performance test results of skincare composition A III. Self-evaluation test: 100 healthy women aged 20-45 were recruited as subjects. Subjects used the skin care composition A prepared in this invention on their face once in the morning and once in the evening. On the 28th day of use (D28), a questionnaire was used to conduct subjective evaluations (not obvious, obvious, very obvious) on whether the use of skin care composition A improved skin dryness, redness, tightness, irritation, improved skin elasticity, reduced fine lines, and whether the skin was smoother, more delicate and firmer. This was to help evaluate the moisturizing, repairing, firming and anti-wrinkle effects of the product. The self-assessment results are shown in Table 3 below; Table 3. Self-assessment results of subjects after using skincare composition A IV. Safety Assessment: 100 healthy women aged 25-55 were recruited as subjects. The subjects used the skin care composition A prepared in this invention on their faces once in the morning and once in the evening. On the 28th day of use (D28), a questionnaire was used to investigate whether there were any adverse reactions such as irritation or allergies after using skin care composition A. The test results showed that the subjects did not experience any adverse reactions such as irritation or allergies.

[0020] Example 4: A skin care composition B has the following raw material formula as shown in Table 4 below; Table 4 Formulation of Skin Care Composition B The preparation method of skin care composition B includes the following steps: Olive oil, isooctyl palmitate, and cetyl alcohol were mixed evenly to obtain component one. Carbomer 941, glycerol, triethanolamine, Tween 60, and 1,3-propanediol were mixed evenly to obtain component two. Component one and component two were heated in a water bath at 70°C and stirred until dissolved. Component one was then added to component two and mixed evenly. The system temperature was lowered to 50°C, and asiaticoside-loaded microspheres, p-hydroxyacetophenone, and 1,2-hexanediol were added and stirred evenly. Finally, ultrapure water was added, and the mixture was emulsified at 800 r / min for 20 min to prepare skin care composition B. Performance testing: I. Sensory and physicochemical evaluation: The appearance, pH, heat resistance, cold resistance and centrifugal stability of the skin care composition B prepared in this invention were evaluated according to GB / T 29665-2013 "Skin Care Lotions". II. Evaluation of Hygiene Indicators: Using the skin care composition B prepared in this invention as a sample, the total number of colonies was evaluated using lecithin Tween 80 nutrient agar medium. 1 mL of test solution diluted to 1:10 and 1:100 (sample: distilled water) was added to the petri dishes, and then 15 mL of sterilized lecithin Tween 80 nutrient agar medium was injected. The mixture was stirred well. Two groups of test solutions were prepared for each dilution. Sterilized petri dishes without test solution were prepared using the same method as blank control groups. The petri dishes were placed in an incubator at (36±1)℃ and incubated for (48±2) h. The total number of colonies on the culture medium was observed after 48 h. The test results are shown in Table 5 below; Table 5 Performance test results of skincare composition B III. Self-evaluation test: 100 healthy women aged 25-55 were recruited as subjects. Subjects used the skin care composition B prepared in this invention on their face once in the morning and once in the evening every day. On the 28th day of use (D28), a questionnaire was used to conduct subjective evaluations (not obvious, obvious, very obvious) on whether the use of skin care composition B improved skin dryness, redness, tightness, irritation, skin elasticity, and fine lines, and whether the skin was smoother, more delicate and firmer. This was to help evaluate the moisturizing, repairing, firming and anti-wrinkle effects of the product. The self-assessment results are shown in Table 6 below; Table 6. Self-assessment results of subjects after using skincare composition B IV. Safety Assessment: 100 healthy women aged 25-55 were recruited as subjects. The subjects used the skin care composition B prepared in this invention on their faces once in the morning and once in the evening. On the 28th day of use (D28), a questionnaire was used to investigate whether there were any adverse reactions such as irritation or allergies after using skin care composition B. The test results showed that the subjects did not experience any adverse reactions such as irritation or allergies.

[0021] Example 5: A skin care composition C has the following raw material formula as shown in Table 1 below; Table 7 Formulation of Skin Care Composition C The preparation method of skin care composition C includes the following steps: Olive oil, isooctyl palmitate, and cetyl alcohol were mixed evenly to obtain component one. Carbomer 941, glycerol, triethanolamine, Tween 60, and 1,3-propanediol were mixed evenly to obtain component two. Component one and component two were heated in a water bath at 70°C and stirred until dissolved. Component one was then added to component two and mixed evenly. The system temperature was lowered to 50°C, and asiaticoside microspheres, p-hydroxyacetophenone, and 1,2-hexanediol were added and stirred evenly. Finally, ultrapure water was added, and the mixture was emulsified at 800 r / min for 20 min to prepare skin care composition C. Performance testing: I. Sensory and physicochemical evaluation: The appearance, pH, heat resistance, cold resistance and centrifugal stability of the skin care composition C prepared in this invention were evaluated according to GB / T 29665-2013 "Skin Care Lotions". II. Evaluation of Hygiene Indicators: Using the skin care composition C prepared in this invention as a sample, the total number of colonies was evaluated using lecithin Tween 80 nutrient agar medium. 1 mL of test solution diluted to 1:10 and 1:100 (sample: distilled water) was added to the petri dishes, and then 15 mL of sterilized lecithin Tween 80 nutrient agar medium was injected. The mixture was stirred well. Two groups of test solutions were prepared for each dilution. Sterilized petri dishes without test solution were prepared using the same method as blank control groups. The petri dishes were placed in an incubator at (36±1)℃ and incubated for (48±2) h. The total number of colonies on the culture medium was observed after 48 h. The test results are shown in Table 8 below; Table 8 Performance test results of skincare composition C III. Self-evaluation test: 100 healthy women aged 25-55 were recruited as subjects. Subjects used the skin care composition C prepared in this invention on their face once in the morning and once in the evening. On the 28th day of use (D28), a questionnaire was used to conduct subjective evaluations (not obvious, obvious, very obvious) on whether the use of skin care composition C improved skin dryness, redness, tightness, irritation, improved skin elasticity, reduced fine lines, and whether the skin was smoother, more delicate and firmer. This was to help evaluate the moisturizing, repairing, firming and anti-wrinkle effects of the product. The self-assessment results are shown in Table 9 below; Table 9 Self-assessment results of subjects after using skincare composition C IV. Safety Assessment: 100 healthy women aged 25-55 were recruited as subjects. The subjects used the skin care composition C prepared in this invention on their faces once in the morning and once in the evening. On the 28th day of use (D28), a questionnaire was used to investigate whether there were any adverse reactions such as irritation or allergies after using the skin care composition C. The test results showed that the subjects did not experience any adverse reactions such as irritation or allergies.

[0022] Example 6: A skin care composition D has the following raw material formulation as shown in Table 10; Table 10 Formulation of Skin Care Composition D The preparation method of skin care composition D includes the following steps: Olive oil, isooctyl palmitate, and cetyl alcohol were mixed evenly to obtain component one. Carbomer 941, glycerol, triethanolamine, Tween 60, and 1,3-propanediol were mixed evenly to obtain component two. Component one and component two were heated in a water bath at 70°C and stirred until dissolved. Component one was then added to component two and mixed evenly. The system temperature was lowered to 50°C, and asiaticoside microspheres, p-hydroxyacetophenone, and 1,2-hexanediol were added and stirred evenly. Finally, ultrapure water was added, and the mixture was emulsified at 800 r / min for 20 min to prepare skin care composition D. Performance testing: I. Sensory and physicochemical evaluation: The appearance, pH, heat resistance, cold resistance and centrifugal stability of the skin care composition D prepared in this invention were evaluated according to GB / T 29665-2013 "Skin Care Lotions". II. Evaluation of Hygiene Indicators: Using the skin care composition D prepared in this invention as a sample, the total number of colonies was evaluated using lecithin Tween 80 nutrient agar medium. 1 mL of test solution diluted to 1:10 and 1:100 (sample: distilled water) was added to the petri dishes, and then 15 mL of sterilized lecithin Tween 80 nutrient agar medium was injected. The mixture was stirred well. Two groups of test solutions were prepared for each dilution. Sterilized petri dishes without test solution were prepared using the same method as blank control groups. The petri dishes were placed in an incubator at (36±1)℃ and incubated for (48±2) h. The total number of colonies on the culture medium was observed after 48 h. The test results are shown in Table 11 below; Table 11 Performance test results of skincare composition D III. Self-evaluation test: 100 healthy women aged 25-55 were recruited as subjects. The subjects used the skin care composition D prepared in this invention on their face once in the morning and once in the evening every day. On the 28th day of use (D28), a questionnaire was used to conduct subjective evaluations (not obvious, obvious, very obvious) on whether the use of skin care composition D improved skin dryness, redness, tightness, irritation, improved skin elasticity, reduced fine lines, and whether the skin was smoother, more delicate and firmer. This was to help evaluate the moisturizing, repairing, firming and anti-wrinkle effects of the product. The self-assessment results are shown in Table 12 below; Table 12 Self-assessment results of subjects after using skincare composition D IV. Safety Assessment: 100 healthy women aged 25-55 were recruited as subjects. The subjects used the skin care composition D prepared in this invention on their faces once in the morning and once in the evening. On the 28th day of use (D28), a questionnaire was used to investigate whether there were any adverse reactions such as irritation or allergies after using skin care composition D. The test results showed that the subjects did not experience any adverse reactions such as irritation or allergies.

[0023] Example 7: A skin care composition E has the following raw material formula as shown in Table 13 below; Table 13 Formulation of Skin Care Composition E The preparation method of skin care composition E includes the following steps: Olive oil, isooctyl palmitate, and cetyl alcohol were mixed evenly to obtain component one. Carbomer 941, glycerol, triethanolamine, Tween 60, and 1,3-propanediol were mixed evenly to obtain component two. Component one and component two were heated in a water bath at 70°C and stirred until dissolved. Component one was then added to component two and mixed evenly. The system temperature was lowered to 50°C, and asiaticoside microspheres, p-hydroxyacetophenone, and 1,2-hexanediol were added and stirred evenly. Finally, ultrapure water was added, and the mixture was emulsified at 800 r / min for 20 min to prepare skin care composition E. Performance testing: I. Sensory and physicochemical evaluation: The appearance, pH, heat resistance, cold resistance and centrifugal stability of the skin care composition E prepared in this invention were evaluated according to GB / T 29665-2013 "Skin Care Lotions". II. Evaluation of Hygiene Indicators: Using the skin care composition E prepared in this invention as a sample, the total number of colonies was evaluated using lecithin Tween 80 nutrient agar medium. 1 mL of test solution diluted to 1:10 and 1:100 (sample: distilled water) was added to the petri dishes, and then 15 mL of sterilized lecithin Tween 80 nutrient agar medium was injected. The mixture was stirred well. Two groups of test solutions were prepared for each dilution. Sterilized petri dishes without test solution were prepared using the same method as blank control groups. The petri dishes were placed in an incubator at (36±1)℃ and incubated for (48±2) h. The total number of colonies on the culture medium was observed after 48 h. The test results are shown in Table 14 below; Table 14 Performance test results of skincare composition E III. Self-evaluation test: 100 healthy women aged 25-55 were recruited as subjects. Subjects used the skin care composition E prepared in this invention on their face once in the morning and once in the evening every day. On the 28th day of use (D28), a questionnaire was used to conduct subjective evaluations (not obvious, obvious, very obvious) on whether the use of skin care composition E improved skin dryness, redness, tightness, irritation, improved skin elasticity, reduced fine lines, and whether the skin was smoother, more delicate and firmer. This was to help evaluate the moisturizing, repairing, firming and anti-wrinkle effects of the product. The self-assessment results are shown in Table 15 below; Table 15 Self-assessment results of subjects after using skincare composition E IV. Safety Assessment: 100 healthy women aged 25-55 were recruited as subjects. The subjects used the skin care composition E prepared in this invention on their faces once in the morning and once in the evening. On the 28th day of use (D28), a questionnaire was used to investigate whether there were any adverse reactions such as irritation or allergies after using skin care composition E. The test results showed that the subjects did not experience any adverse reactions such as irritation or allergies.

Claims

1. A skincare composition, characterized in that, The ingredients include the following parts by weight: 1-10 portions of asiaticoside-loaded microspheres, using an amphiphilic monomer as a carrier, with the mass ratio of the carrier to the active component asiaticoside being 1:(4.0-4.5); 1-10 portions of asiaticoside microspheres, using an amphiphilic monomer as a carrier, with a mass ratio of the carrier to the active component asiaticoside of 1:(4.0-4.5); 2-4 parts olive oil; 3-5 parts isooctyl palmitate; 2-4 parts cetyl alcohol; 0.2-0.4 parts carbomer; 1-3 parts glycerol; 0.2-0.4 parts triethanolamine; 0.3-0.5 parts Tween 60; 1-3 parts of 1,3-propanediol; 0.4-0.5 parts p-hydroxyacetophenone; 0.01-0.1 parts of 1,2-hexanediol; 60-85 parts ultrapure water.

2. The skincare composition according to claim 1, characterized in that, The chemical structure of the amphiphilic monomer is as follows: 。 3. The method for preparing a skincare composition according to claim 1, characterized in that, Includes the following steps: Step 1: Mix olive oil, isooctyl palmitate, and cetyl alcohol evenly to obtain component 1; Step 2: Mix carbomer, glycerol, triethanolamine, Tween 60 and 1,3-propanediol evenly to obtain component 2; Step 3: Heat components 1 and 2 separately in a water bath at 60-80℃ and stir until dissolved. Then add component 1 to component 2 and mix well. Reduce the system temperature to 45-55℃, add asiaticoside-loaded microspheres, p-hydroxyacetophenone and 1,2-hexanediol, stir evenly, and finally add ultrapure water to emulsify and prepare the skin care composition.

4. A method for preparing a skincare composition according to claim 3, characterized in that, The emulsification time is 10-30 minutes.

5. The method for preparing a skincare composition according to claim 3, characterized in that, The method for preparing the hydroxyasiaticoside-loaded microspheres is as follows: utilizing the amphiphilic properties of amphiphilic monomers, a chamber structure with an internal hydrophobic and external hydrophilic structure is formed through self-assembly, and hydroxyasiaticoside is coated to prepare hydroxyasiaticoside-loaded microspheres.

6. The method for preparing a skincare composition according to claim 3, characterized in that, The method for preparing the asiaticoside-loaded microspheres is as follows: utilizing the amphiphilic properties of amphiphilic monomers, a chamber structure with an internal hydrophobic and external hydrophilic structure is formed through self-assembly, and asiaticoside enters the chamber to prepare asiaticoside-loaded microspheres.

7. A method for preparing a skincare composition according to claim 5 or 6, characterized in that, The preparation method of the amphiphilic monomer is as follows: Based on the hydroxy-carboxyl esterification mechanism, ethylenediaminetetraacetic acid (EDTA) reacts with lauryl alcohol in a 1:1 molar ratio under the catalysis of protic acid to prepare EDTA lauryl ester. Based on the hydroxy-carboxyl esterification reaction mechanism, ethylenediaminetetraacetic acid lauryl ester and sorbitol react in a molar ratio of 1:3 to prepare sorbitol-based ethylenediaminetetraacetic acid lauryl ester. Based on the quaternization reaction mechanism, sorbitol ethylenediaminetetraacetic acid lauryl ester reacts with 1-chlorododecane in a molar ratio of 1:2 to prepare an amphiphilic monomer.

8. The method for preparing a skincare composition according to claim 6, characterized in that, The protic acid is either p-toluenesulfonic acid or trifluoromethanesulfonic acid.

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