Ultraviolet modified solid plasma matrix as well as preparation method and application thereof

Abstract

The invention provides an ultraviolet modified solid plasma matrix as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method of the ultraviolet modified solid plasma matrix comprises the following steps: irradiating a solid plasma matrix blood collection tube by using first ultraviolet light to obtain a modified solid plasma matrix blood collection tube; collecting a blood sample for separation, and irradiating with second ultraviolet light at the same time to form a solid plasma matrix precursor; third ultraviolet light is used for irradiation, and the ultraviolet modified solid plasma matrix is obtained. According to the invention, ultraviolet light treatment is introduced in the whole preparation process of the solid plasma matrix, and ultraviolet light treatment with different wavelengths is performed in a targeted manner according to the characteristics of ultraviolet light and the preparation stage of the solid plasma matrix, so that the preparation success rate of the solid plasma matrix is improved; and the obtained ultraviolet modified solid plasma matrix is higher in mechanical strength after film formation, longer in degradation time and higher in yield, and has important application value in the aspect of soft tissue regeneration.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an ultraviolet-modified solid plasma matrix and a preparation method and application thereof. Background Art

[0002] In modern regenerative medicine, plasma matrix has been increasingly widely used in clinical practice to further promote wound healing and tissue regeneration. Studies have shown that platelets play an important role in angiogenesis, local inflammation regulation, tissue regeneration, etc. Platelets separated from peripheral blood contain more than 1,500 autologous bioactive components, including growth factors, immune system signaling factors, and enzymes, which play a vital role in tissue repair and wound healing. The plasma matrix can provide 6-8 times the physiological dose of growth factors to stimulate wound healing. In addition, the growth factors released by platelets can recruit cells required for the healing process and stimulate the differentiation of mesenchymal stem cells.

[0003] Early studies have found that there are several key growth factors in platelets that can significantly promote tissue regeneration. Based on this result, plasma matrix has been developed in regenerative medicine for more than two decades, and has been upgraded and iterated several times in preparation schemes, product distribution and content. Plasma matrix is ​​a method of concentrating human plasma through centrifugation and other schemes to produce supraphysiological concentrations of autologous platelets, which are then used clinically. The first generation of plasma matrix (platelet-rich plasma) requires multiple steps of centrifugation, the addition of non-autologous anticoagulants, and the additional use of bovine thrombin or calcium chloride to promote coagulation. In addition, platelet-rich plasma technology focuses mainly on achieving enrichment of platelets and growth factors, aiming to eliminate leukocytes from the final components. However, anticoagulants work by preventing coagulation and fibrin clot formation, which has a negative impact on the healing process, which has prompted the evolution of the next generation of plasma matrix.

[0004] In 2001, some scholars introduced a new plasma matrix preparation scheme, called platelet-rich fibrin, which can obtain a completely autologous solid plasma matrix that does not contain any anticoagulants and contains a high concentration of host immune cells. However, the solid plasma matrix prepared by this scheme has significant differences between patients. According to clinical statistics, more than 30% of blood samples cannot directly form a solid plasma matrix after centrifugation and require secondary centrifugation, which significantly increases the clinical operation time. At the same time, the final product cannot meet the requirements of clinical soft tissue regeneration in terms of yield, mechanical strength, degradation time, etc. Summary of the invention

[0005] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a UV-modified solid plasma matrix and a preparation method and application thereof. Compared with the traditional method for preparing the solid plasma matrix, the preparation method provided by the present invention can shorten the preparation time of the solid plasma matrix and improve the success rate of the preparation of the solid plasma matrix; and the obtained UV-modified solid plasma matrix has higher mechanical strength after film formation, longer degradation time, and higher yield, and has important application value in soft tissue regeneration.

[0006] To achieve this object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a method for preparing a UV-modified solid plasma matrix, the preparation method comprising the following steps:

[0008] (1) irradiating the solid plasma matrix blood collection tube with a first ultraviolet light to obtain a modified solid plasma matrix blood collection tube;

[0009] (2) separating the blood sample in the modified solid plasma matrix blood collection tube and irradiating it with a second ultraviolet light to form a solid plasma matrix precursor;

[0010] (3) Using a third ultraviolet light to irradiate the solid plasma matrix precursor to obtain an ultraviolet-modified solid plasma matrix.

[0011] The preparation method of the ultraviolet modified solid plasma matrix provided by the present invention comprises three stages, namely: pre-modification, induction and reactivation.

[0012] Among them, step (1) is a pre-modification stage, which can be carried out in a plasma matrix incubator. The pre-modification stage utilizes the high penetrability of low-frequency long-wave ultraviolet light to pre-modify the solid plasma matrix blood collection tube, which can increase the content of hydrophilic groups on the inner wall surface of the solid plasma matrix blood collection tube, improve the hydrophilicity of the solid plasma matrix blood collection tube, help stimulate platelet activation, promote the cross-linking of fibrin in the plasma matrix, and promote the coagulation of the plasma matrix.

[0013] Step (2) is the induction start-up stage, in which the cap of the blood collection tube can be opened and carried out in the plasma matrix preparation platform. The induction start-up stage utilizes the high energy characteristics of extreme ultraviolet light to, on the one hand, trigger and stimulate internal fiber cross-linking during the preparation of the solid plasma matrix, making the fibrin network denser, improving the mechanical strength of the subsequently prepared UV-modified solid plasma matrix membrane, and prolonging the degradation time; on the other hand, it can also ensure the safety of the preparation process and avoid the risk of cross infection.

[0014] Step (3) is the reactivation stage, in which the blood collection tube cap can be opened and carried out in a plasma matrix incubator. The blood collection tube obtained after step (2) is subjected to step (3) as a whole (together with the material in the tube), and the ultraviolet-modified solid plasma matrix is ​​separated after completion. The ultraviolet light irradiation in the reactivation stage can play a sterilizing role and maintain the safety of the product. At the same time, the ultraviolet light in this band can generate ozone, stimulate the activation of platelets, and further promote the coagulation of the plasma matrix.

[0015] The present invention introduces ultraviolet light treatment during the entire preparation process of the solid plasma matrix, and provides targeted ultraviolet light treatment of different wavelengths according to the characteristics of the ultraviolet light and the stage of preparation of the solid plasma matrix, thereby improving the preparation success rate of the solid plasma matrix and shortening the preparation time. The obtained ultraviolet-modified solid plasma matrix has higher mechanical strength after film formation, longer degradation time, and higher yield. In soft tissue regeneration, it can provide a good barrier function and promote vascularization and tissue regeneration.

[0016] In some embodiments of the present invention, the wavelength of the first ultraviolet light is 320-400 nm, for example, 320 nm, 330 nm, 340 nm, 350 nm, 360 nm, 370 nm, 380 nm, 390 nm or 400 nm, etc.; the irradiation time is more than 5 min, for example, 5 min, 6 min, 8 min, 10 min, 15 min, 20 min, 25 min, 30 min, 40 min, 50 min or 60 min, etc. However, the present invention is not limited to the listed values, and other values ​​not listed within the range are also applicable.

[0017] In the present invention, if the wavelength of the first ultraviolet light is too short, its penetration is reduced and it may not be able to effectively modify the inside of the blood collection tube; if the wavelength of the first ultraviolet light is too long, its energy is too low and it may not meet the modification requirements. If the irradiation time of the first ultraviolet light is too short, the degree of modification inside the blood collection tube is low, the improvement effect on the solid plasma matrix is ​​reduced, or even no improvement effect is achieved.

[0018] In some embodiments of the present invention, the wavelength of the second ultraviolet light is 10-121 nm, for example, 10 nm, 20 nm, 30 nm, 40 nm, 50 nm, 60 nm, 70 nm, 80 nm, 90 nm, 100 nm, 110 nm, 120 nm or 121 nm, etc.; the irradiation time is more than 5 s, preferably 5 s-20 min, for example, 5 s, 10 s, 15 s, 20 s, 30 s, 40 s, 50 s, 60 s, 2 min, 3 min, 5 min, 8 min, 10 min, 12 min, 15 min, 18 min or 20 min, etc. However, the present invention is not limited to the listed values, and other values ​​not listed within the range are also applicable.

[0019] In the present invention, if the wavelength of the second ultraviolet light is too long or the irradiation time is too short, the coagulation effect of the plasma matrix will be reduced, and the mechanical strength of the final ultraviolet-modified solid plasma matrix membrane will be reduced.

[0020] In some embodiments of the present invention, the wavelength of the third ultraviolet light is 100-280nm, for example, it can be 100nm, 120nm, 140nm, 160nm, 180nm, 200nm, 220nm, 240nm, 260nm or 280nm, etc.; the irradiation time is more than 1min, preferably 1-60min, for example, it can be 1min, 2min, 3min, 5min, 8min, 10min, 12min, 15min, 18min, 20min, 25min, 30min, 40min, 50min or 60min, etc. However, the present invention is not limited to the listed values, and other values ​​not listed in the range are also applicable.

[0021] In the present invention, the wavelength of the third ultraviolet light is within the above range, which is more conducive to the generation of ozone and the sterilization effect. If the wavelength of the third ultraviolet light is too short or too long, or the irradiation time is too short, it may lead to a decrease in ozone production, resulting in a decrease in the coagulation effect of the plasma matrix, and a decrease in the mechanical strength of the final ultraviolet-modified solid plasma matrix membrane.

[0022] In some embodiments of the present invention, the material of the solid plasma matrix blood collection tube is glass. In the present invention, the UV-modified solid plasma matrix membrane prepared by using a solid plasma matrix blood collection tube made of glass has the best effect. If a solid plasma matrix blood collection tube made of plastic material (such as PET) is used, although it can also play a certain improvement role, the performance of the prepared UV-modified solid plasma matrix membrane may still be difficult to meet the requirements of clinical application.

[0023] In some embodiments of the present invention, the separation method in step (2) is centrifugation, magnetic bead adsorption or filtration, preferably centrifugation.

[0024] In some embodiments of the present invention, the centrifugal acceleration of the centrifugation is 50-3000g, for example, 50g, 60g, 80g, 100g, 150g, 200g, 250g, 300g, 400g, 500g, 600g, 700g, 800g, 900g, 1000g, 1500g, 2000g, 2500g or 3000g, etc.; the centrifugal time is 1-20min, for example, 1min, 2min, 3min, 5min, 6min, 8min, 10min, 12min, 15min, 18min or 20min, etc. However, the present invention is not limited to the listed values, and other values ​​not listed within the range are also applicable.

[0025] In a second aspect, the present invention provides a UV-modified solid plasma matrix prepared by the preparation method described in the first aspect.

[0026] In a third aspect, the present invention provides a UV-modified solid plasma matrix membrane obtained by pressing the UV-modified solid plasma matrix described in the second aspect.

[0027] In some embodiments of the present invention, the pressing pressure is 0.5-5N, for example, 0.5N, 0.8N, 1N, 1.5N, 2N, 2.5N, 3N, 3.5N, 4N, 4.5N or 5N, etc.; the pressing time is 30s-5min, for example, 30s, 40s, 50s, 1min, 1.5min, 2min, 2.5min, 3min, 3.5min, 4min, 4.5min or 5min, etc. However, the present invention is not limited to the listed values, and other values ​​not listed in the range are also applicable.

[0028] In a fourth aspect, the present invention provides a use of the UV-modified solid plasma matrix as described in the second aspect or the UV-modified solid plasma matrix membrane as described in the third aspect as a soft tissue repair material.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The preparation method provided by the present invention introduces ultraviolet light treatment during the entire preparation process of the solid plasma matrix, and according to the characteristics of the ultraviolet light and the stage of preparation of the solid plasma matrix, ultraviolet light treatment of different wavelengths is given in a targeted manner, thereby improving the preparation success rate of the solid plasma matrix and shortening the preparation time; and the obtained ultraviolet modified solid plasma matrix has higher mechanical strength after film formation, longer degradation time, and higher yield, and can provide a good barrier function in soft tissue regeneration and promote vascularization and tissue regeneration. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The hydrophilicity test photos of the modified solid plasma matrix blood collection tube and the unmodified solid plasma matrix blood collection tube prepared in Example 1;

[0032] Figure 2 These are photos of the UV-modified solid plasma matrix prepared in Example 1 and the solid plasma matrix prepared in Comparative Example 1. DETAILED DESCRIPTION

[0033] The technical solution of the present invention is further described below with reference to the accompanying drawings and through specific implementations. It should be understood by those skilled in the art that the specific implementations are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0034] Example 1

[0035] This embodiment provides a UV-modified solid plasma matrix membrane, and the preparation method thereof is as follows:

[0036] (1) preparing 4 solid plasma matrix blood collection tubes (made of glass), pre-modifying them in a plasma matrix incubator, setting the ultraviolet light wavelength to 400 nm, and irradiating for 20 minutes to obtain modified solid plasma matrix blood collection tubes;

[0037] (2) using the modified solid plasma matrix blood collection tube obtained in step (1) to collect 40 mL of human blood, with each blood collection tube containing 10 mL, releasing the blood collection tube cap, and quickly placing it in the plasma matrix preparation platform, setting the ultraviolet light wavelength to 10 nm and the centrifugal acceleration to 1500 g, centrifuging and irradiating for 5 minutes at the same time, and forming a solid plasma matrix precursor on the upper layer of the blood;

[0038] (3) After step (2), the whole blood collection tube is transferred to a plasma matrix incubator for reactivation, the ultraviolet light wavelength is set to 200 nm, irradiated for 10 minutes, and the upper layer of ultraviolet-modified solid plasma matrix is ​​separated;

[0039] (4) The UV-modified solid plasma matrix obtained in step (3) is pressed using a mold at a pressure of 1 N for 2 minutes until the thickness is 2 mm, thereby obtaining a UV-modified solid plasma matrix membrane.

[0040] Example 2

[0041] This embodiment provides a UV-modified solid plasma matrix membrane, and the preparation method thereof is different from that of embodiment 1 in that:

[0042] The wavelength of ultraviolet light in step (1) is 350 nm and the irradiation time is 30 min;

[0043] The ultraviolet light wavelength in step (2) is 20 nm, the centrifugal acceleration is 700 g, and the centrifugation and irradiation time is 8 min;

[0044] The wavelength of the ultraviolet light in step (3) is 150 nm and the irradiation time is 8 min.

[0045] Example 3

[0046] This embodiment provides a UV-modified solid plasma matrix membrane, and the preparation method thereof is different from that of embodiment 1 in that:

[0047] The wavelength of ultraviolet light in step (1) is 320 nm and the irradiation time is 5 min;

[0048] The ultraviolet light wavelength in step (2) is 121 nm, the centrifugal acceleration is 500 g, and the centrifugation and irradiation time is 20 min;

[0049] The wavelength of the ultraviolet light in step (3) is 280 nm and the irradiation time is 60 min.

[0050] Example 4

[0051] This embodiment provides a UV-modified solid plasma matrix membrane, and the preparation method thereof is different from that of embodiment 1 in that:

[0052] The ultraviolet light wavelength in step (1) is 380 nm and the irradiation time is 10 min;

[0053] The ultraviolet light wavelength in step (2) is 50 nm, the centrifugal acceleration is 2000 g, the centrifugal time is 3 min, and the irradiation time is 5 s;

[0054] The wavelength of the ultraviolet light in step (3) is 100 nm and the irradiation time is 1 min.

[0055] Comparative Example 1

[0056] This comparative example provides a solid plasma matrix membrane, and the preparation method thereof is as follows:

[0057] 40 mL of human blood was collected using 4 solid plasma matrix blood collection tubes, each with 10 mL, and quickly placed in the plasma matrix preparation platform. The centrifugal acceleration was set to 1500 g and centrifuged for 5 minutes to separate the upper solid plasma matrix;

[0058] The solid plasma matrix was pressed using a mold at a pressure of 1 N for 2 minutes until the thickness was 2 mm, thereby obtaining a solid plasma matrix membrane.

[0059] Comparative Example 2

[0060] This comparative example provides a UV-modified solid plasma matrix membrane, and the preparation method thereof is different from that of Example 1 in that: step (1) does not involve UV irradiation.

[0061] Comparative Example 3

[0062] This comparative example provides a UV-modified solid plasma matrix membrane, and the preparation method thereof is different from that of Example 1 in that: step (2) does not involve UV irradiation.

[0063] Comparative Example 4

[0064] This comparative example provides a UV-modified solid plasma matrix membrane, and the preparation method thereof is different from that of Example 1 in that: step (3) does not involve UV irradiation.

[0065] Comparative Example 5

[0066] This comparative example provides a UV-modified solid plasma matrix membrane, and its preparation method is different from that of Example 1 in that: 500 nm light is used for irradiation in step (1).

[0067] Comparative Example 6

[0068] This comparative example provides a UV-modified solid plasma matrix membrane, and the preparation method thereof is different from that of Example 1 in that the UV light irradiation time in step (1) is 3 minutes.

[0069] Comparative Example 7

[0070] This comparative example provides a UV-modified solid plasma matrix membrane, and its preparation method is different from that of Example 1 in that the wavelength of the UV light in step (2) is 200 nm.

[0071] Comparative Example 8

[0072] This comparative example provides a UV-modified solid plasma matrix membrane, and the preparation method thereof is different from that of Example 1 in that the UV irradiation time in step (2) is 2 s (the centrifugation time remains unchanged).

[0073] Comparative Example 9

[0074] This comparative example provides a UV-modified solid plasma matrix membrane, and its preparation method is different from that of Example 1 in that the wavelength of the UV light in step (3) is 50 nm.

[0075] Comparative Example 10

[0076] This comparative example provides a UV-modified solid plasma matrix membrane, and its preparation method is different from that of Example 1 in that the wavelength of the UV light in step (3) is 350 nm.

[0077] Comparative Example 11

[0078] This comparative example provides a UV-modified solid plasma matrix membrane, and its preparation method is different from that of Example 1 in that the UV irradiation time in step (3) is 40 seconds.

[0079] Performance Testing

[0080] 1. Hydrophilicity test: Take the modified solid plasma matrix blood collection tube prepared in Example 1 and the unmodified solid plasma matrix blood collection tube, place the tube body horizontally, use a 1 mL syringe, vertically drop a drop of double distilled water at a distance of 20 mm from the tube mouth and a height of 5 mm, observe the spread of the droplet on the inner wall of the tube, and record the results. Figure 1 shown.

[0081] from Figure 1It can be seen that compared with the unmodified solid plasma matrix blood collection tube, the contact angle between the liquid in the modified solid plasma matrix blood collection tube and the tube wall is significantly reduced, indicating that the hydrophilicity of the modified solid plasma matrix blood collection tube has been significantly improved.

[0082] 2. Coagulation effect: The UV-modified solid plasma matrix prepared in Example 1 and the solid plasma matrix prepared in Comparative Example 1 were taken to observe the coagulation effect. The results are as follows: Figure 2 shown.

[0083] from Figure 2 It can be seen that compared with the solid plasma matrix prepared in Comparative Example 1, the UV-modified solid plasma matrix prepared in Example 1 has a significantly higher yield and a better coagulation effect.

[0084] 2. Coagulation incidence: The example and comparative example were repeated for multiple times, and the proportion of blood coagulation (sample size n=60) was recorded as the coagulation incidence.

[0085] 3. Area of ​​solid plasma matrix membrane: Measure the area of ​​solid plasma matrix membrane prepared from 10 mL of blood.

[0086] 4. Breaking strength: The test was performed on a universal tensile testing machine, with the distance between the upper and lower clamps fixed at 3 mm. The solid plasma matrix membranes provided in the embodiment and the comparative example were cut into the same width (6 mm), and the upper and lower ends thereof were fixed in the upper and lower clamps, respectively, and stretched at a speed of 1 mm / s. The tensile strength when the solid plasma matrix membrane broke was recorded as its breaking strength. Six parallel specimens were taken for each sample, and the test results were averaged.

[0087] The test results are shown in Table 1.

[0088] Table 1

[0089]

[0090]

[0091] From the test results in Table 1, it can be seen that compared with the method for preparing solid plasma matrix by traditional centrifugation (Comparative Example 1), the method provided in the embodiment of the present invention significantly improves the incidence of blood coagulation, the area of ​​the solid plasma matrix membrane prepared from the same volume (10 mL) of blood is significantly increased, and the fracture strength of the solid plasma matrix membrane is significantly improved. This shows that the method provided by the present invention can improve the preparation success rate and yield of the solid plasma matrix (helps to shorten the preparation time) and improve the mechanical strength of the solid plasma matrix membrane (helps to extend the degradation time).

[0092] Among them, compared with Example 1, Comparative Examples 2, 3, and 4 do not perform ultraviolet light irradiation in step (1), step (2), and step (3), respectively, resulting in a decrease in the effect of ultraviolet treatment, thereby reducing the success rate of preparation of the solid plasma matrix, as well as the yield and mechanical strength of the solid plasma matrix membrane.

[0093] Compared with Example 1, the ultraviolet light wavelength used in step (1) of Comparative Example 5 is longer and the energy is lower, and the ultraviolet light irradiation time in step (1) of Comparative Example 6 is shorter, both of which result in a lower degree of modification of the blood collection tube, thereby reducing the success rate of preparation of the solid plasma matrix, as well as the yield and mechanical strength of the solid plasma matrix membrane.

[0094] Compared with Example 1, the ultraviolet light wavelength used in step (2) of Comparative Example 7 is longer and the energy is lower, and the ultraviolet light irradiation time in step (2) of Comparative Example 8 is shorter, both of which lead to a decrease in the coagulation effect of the plasma matrix, thereby reducing the success rate of preparing the solid plasma matrix, as well as the yield and mechanical strength of the solid plasma matrix membrane.

[0095] Compared with Example 1, the ultraviolet light wavelength used in step (3) of Comparative Example 9 is shorter, the ultraviolet light wavelength used in step (3) of Comparative Example 10 is longer, and the ultraviolet light irradiation time in step (3) of Comparative Example 11 is shorter, all of which result in lower ozone production and reduced coagulation effect of the plasma matrix, thereby reducing the success rate of preparation of the solid plasma matrix, as well as the yield and mechanical strength of the solid plasma matrix membrane.

[0096] The above description is only a specific embodiment of the present disclosure, so that those skilled in the art can understand or implement the present disclosure. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present disclosure. Therefore, the present disclosure will not be limited to the embodiments described herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing a UV-modified solid plasma matrix, characterized in that: The preparation method comprises the following steps: (1) irradiating the solid plasma matrix blood collection tube with a first ultraviolet light to obtain a modified solid plasma matrix blood collection tube; (2) separating the blood sample in the modified solid plasma matrix blood collection tube and irradiating it with a second ultraviolet light to form a solid plasma matrix precursor; (3) Using a third ultraviolet light to irradiate the solid plasma matrix precursor to obtain an ultraviolet-modified solid plasma matrix.

2. The preparation method according to claim 1, characterized in that: The wavelength of the first ultraviolet light is 320-400 nm, and the irradiation time is more than 5 minutes.

3. The preparation method according to claim 1 or 2, characterized in that: The wavelength of the second ultraviolet light is 10-121 nm, and the irradiation time is more than 5 s, preferably 5 s-20 min.

4. The preparation method according to any one of claims 1 to 3, characterized in that The wavelength of the third ultraviolet light is 100-280 nm, and the irradiation time is more than 1 min, preferably 1-60 min.

5. The preparation method according to any one of claims 1 to 4, characterized in that: The material of the solid plasma matrix blood collection tube is glass.

6. The preparation method according to any one of claims 1 to 5, characterized in that: The separation method in step (2) is centrifugation, magnetic bead adsorption or filtration, preferably centrifugation; Preferably, the centrifugal acceleration of the centrifugation is 50-3000g, and the centrifugal time is 1-20min.

7. A UV-modified solid plasma matrix, characterized in that: The invention is prepared by the preparation method according to any one of claims 1 to 6.

8. A UV-modified solid plasma matrix membrane, characterized in that: The solid plasma matrix modified by ultraviolet light according to claim 7 is obtained by pressing.

9. The ultraviolet modified solid plasma matrix membrane according to claim 8, characterized in that: The pressing pressure is 0.5-5N, and the pressing time is 30s-5min.

10. Use of the UV-modified solid plasma matrix as claimed in claim 7 or the UV-modified solid plasma matrix membrane as claimed in claim 8 or 9 as a soft tissue repair material.

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