Antibacterial quantum dot material as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to an antibacterial quantum dot material as well as a preparation method and application thereof. The invention provides an antibacterial quantum dot material preparation method, which comprises: mixing a zinc source, an alkali source and a solvent, and carrying out microwave oscillation heating to obtain the antibacterial quantum dot material. The antibacterial quantum dot material is synthesized through microwave oscillation heating, microwave oscillation heating can accelerate raw material reaction, the reaction time is short, zinc oxide can be rapidly generated, the generation rate of zinc oxide and the form and size of zinc oxide can be controlled through microwave oscillation heating, and the prepared zinc oxide is small in particle size and uniform in particle size. The preparation method provided by the invention is short in reaction time, few in side reaction, simple to operate, low in cost and high in efficiency, and has a market application prospect.

Description

Technical Field

[0001] The present invention belongs to the field of biomedicine technology, and in particular relates to an antibacterial quantum dot material and a preparation method and application thereof. Background Art

[0002] Existing antibiotics and frequently updated antibacterial drugs induce bacteria and viruses to evolve resistance while killing bacteria, causing many antibiotics and other antibacterial drugs to fail, which has brought great harm to human health. Finding new antimicrobial drugs is an important way to solve the problem of bacterial resistance. Quantum dots are an emerging material with a particle size between 1 and 10 nm. The application of zinc oxide quantum dots (ZnO·QDs) in the biological field is mainly divided into two categories, one is bioluminescence imaging and drug loading, and the other is antibacterial application. Zinc oxide is low in price, abundant in source and has good antibacterial effect, and has high application value.

[0003] For example, there is a prior art that discloses a method for synthesizing zinc oxide quantum dots. The patent uses sodium methoxide as an alkali source, and the obtained zinc oxide quantum dots have high purity and luminescent properties. However, the patent requires the use of a silane coupling agent, and the preparation requires more raw materials, and the process is relatively complicated. In addition, the obtained zinc oxide quantum dots have an irregular shape and a large particle size (particle size is 100 to 500 nm). Summary of the invention

[0004] In view of this, the object of the present invention is to provide an antibacterial quantum dot material and a preparation method and application thereof. The preparation method provided by the present invention can obtain a spherical and uniformly dispersed antibacterial quantum dot material.

[0005] The present invention provides a method for preparing an antibacterial quantum dot material, comprising the following preparation steps:

[0006] The zinc source, the alkali source and the solvent are mixed and subjected to microwave oscillation heating to obtain the antibacterial quantum dot material.

[0007] Preferably, the zinc source includes one or more of anhydrous zinc chloride, anhydrous zinc acetate and zinc acetate dihydrate.

[0008] Preferably, the alkali source is an inorganic alkali source; the inorganic alkali source includes sodium hydroxide and / or potassium hydroxide.

[0009] Preferably, the solvent comprises an ethanol-water system;

[0010] The concentration of ethanol in the ethanol-water system is 0 to 50 wt %.

[0011] Preferably, the volume ratio of the amount of the zinc source to the solvent is 0.01 to 0.2 mol / L.

[0012] Preferably, in the mixed system, the molar ratio of the zinc source to the hydroxide is 1:1-3.

[0013] Preferably, the microwave oscillation heating temperature is 120-180°C and the time is 5-30 min;

[0014] The power of the microwave oscillation heating is 300-800W.

[0015] The present invention also provides an antibacterial quantum dot material prepared by the preparation method described in the above technical solution, wherein the antibacterial quantum dot material is a spherical quantum dot;

[0016] The diameter of the spherical quantum dots is 80-150 nm.

[0017] Preferably, the mass percentage of zinc oxide in the antibacterial quantum dot material is ≥98%.

[0018] The present invention also provides the use of the antibacterial quantum dot material described in the above technical solution in the preparation of antibacterial drugs.

[0019] The present invention provides a method for preparing an antibacterial quantum dot material, comprising the following preparation steps: mixing a zinc source, an alkali source and a solvent, and performing microwave oscillation heating to obtain the antibacterial quantum dot material. The preparation method provided by the present invention uses microwave oscillation heating to synthesize the antibacterial quantum dot material, which can accelerate the chemical reaction of the raw materials, make the particles more evenly dispersed, easier to form a sphere, more uniform in particle size, and the obtained zinc oxide quantum dots have a smaller particle size. It avoids the problems of mutual adsorption and excessive aggregation of zinc oxide during preparation by conventional methods, forming irregular shapes such as flakes or excessive particle size. At the same time, the zinc oxide quantum dots prepared based on this method have antibacterial and anti-drug resistance-reducing biological activity, are important substitutes for anti-resistance reduction, and can lay the foundation for the future transformation and application of zinc oxide quantum dots. The preparation method provided by the present invention has short reaction time, few side reactions, simple operation, low cost, high efficiency, biological activity, and broad market transformation prospects. The yield of the antibacterial quantum dot material prepared by the preparation method provided by the present invention can reach 82.5%, and the purity is ≥98%.

[0020] The antibacterial quantum dot material provided by the present invention is composed of single zinc oxide with a particle size of 5 to 10 nm, and the diameter of the spherical quantum dots is 80 to 150 nm. The particle size of the single zinc oxide in the antibacterial quantum dot material provided by the present invention is less than 10 nm, and the surface of each spherical quantum dot formed by agglomeration is loose, and the particles are evenly dispersed without obvious irregular shapes. In vitro antibacterial and drug resistance reduction tests show that it has significant antibacterial activity, and long-term use will not induce Escherichia coli and Staphylococcus aureus to develop drug resistance. The possible mechanism is that the zinc oxide quantum dots synthesized by this method have a smaller particle size and can better penetrate into the bacteria, thereby destroying the bacterial genetic material DNA, resulting in the inability of the bacteria to divide and propagate, and eventually die. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a SEM image of the zinc oxide antibacterial quantum dot material prepared in Example 1;

[0022] Figure 2 The Zeta potential diagram of the zinc oxide antibacterial quantum dot material prepared in Example 1;

[0023] Figure 3 This is a curve comparison of the minimum inhibitory concentration MIC of the zinc oxide antibacterial quantum dot material prepared in Example 1 against Escherichia coli and Staphylococcus aureus;

[0024] Figure 4 This is the MBC coating diagram of the zinc oxide antibacterial quantum dot material prepared in Example 1 against Escherichia coli and Staphylococcus aureus;

[0025] Figure 5 This is a test diagram of the induced resistance of the zinc oxide antibacterial quantum dot material prepared in Example 1 to vancomycin. DETAILED DESCRIPTION

[0026] The present invention provides a method for preparing an antibacterial quantum dot material, comprising the following steps:

[0027] The zinc source, the alkali source and the solvent are mixed and subjected to microwave oscillation heating to obtain the antibacterial quantum dot material.

[0028] In the present invention, unless otherwise specified, the raw materials used are commercially available products well known to those skilled in the art.

[0029] In the present invention, the zinc source includes one or more of anhydrous zinc chloride, anhydrous zinc acetate and zinc acetate dihydrate, and in a specific embodiment, it can be anhydrous zinc chloride. In the present invention, the purity of the zinc source is analytically pure. In the present invention, the alkali source is an inorganic alkali source, and the inorganic alkali source includes sodium hydroxide and / or potassium hydroxide, and in a specific embodiment, it can be potassium hydroxide. In the present invention, the purity of the alkali source is analytically pure. In the present invention, the alkali source can be used in the form of an alkali source aqueous solution, and the concentration of the alkali source aqueous solution is 0.3-0.7 mol / L, and in a specific embodiment, it can be 0.5 mol / L. In the present invention, the water in the alkali source aqueous solution is deionized water or pure water. The purpose of adding the alkali source in the present invention is to provide hydroxide ions, and the beneficial effect is to help zinc salts form ZnO precipitation in an alcohol system.

[0030] In the present invention, the solvent may be an ethanol-water system. In the present invention, the concentration of ethanol in the ethanol-water system may be 0 to 50 wt%, and in a specific embodiment, may be 30 wt%. The present invention uses an ethanol-water system as a solvent, which is non-toxic; the ethanol aqueous solution itself has certain antibacterial properties; ethanol is easy to separate and will not affect the purity of the antibacterial quantum dot material. The particle size of the antibacterial quantum dot material obtained by the present invention decreases with the increase of the ethanol concentration.

[0031] The preparation raw materials used in the present invention are of single type and high purity, and the obtained antibacterial quantum dot material has high purity.

[0032] In the present invention, the mixing of the zinc source, the alkali source and the solvent comprises: mixing the zinc source with the solvent to obtain a zinc source solution; and adding the alkali source aqueous solution into the zinc source solution for mixing.

[0033] In the present invention, the concentration of the zinc source in the zinc source solution is 0.01-0.2 mol / L, and in a specific embodiment, it can be 0.1 mol / L; the concentration ratio of the zinc source solution to the alkali source aqueous solution can be 0.01-0.2:0.3-0.7, and in a specific embodiment, it can be 0.1:0.5; the volume ratio is 5:1-3, and in a specific embodiment, it can be 5:2 or 5:3.

[0034] In the present invention, in the mixed system, the molar ratio of the zinc source to the hydroxide ion can be 1:1 to 3, and in a specific embodiment, it can be 1:2. In the present invention, the zinc source and the hydroxide ion will undergo an ion reaction, and the present invention limits the molar ratio of the zinc source to the hydroxide ion, which has the effect of enhancing the stability of zinc oxide.

[0035] In the present invention, the temperature of the microwave oscillation heating can be 120-180° C., and in a specific embodiment, it can be 150° C. In the present invention, the microwave oscillation heating time can be 5-30 minutes, and in a specific embodiment, it can be 15 minutes. In the present invention, the power of the microwave oscillation heating can be 300-800W, and in a specific embodiment, it can be 400W or 600W.

[0036] In the present invention, the microwave oscillation heating may be performed under stirring conditions, and the stirring speed may be 400-600 rpm, and in a specific embodiment, may be 500 rpm.

[0037] After the microwave oscillation heating, the present invention further comprises separating, washing and drying the obtained liquid. In the present invention, the separation method may be suction filtration. In the present invention, the washing reagent may be deionized water, and the number of washings may be ≥ 3 times. In the present invention, the drying temperature may be 50 to 80°C, and in a specific embodiment, may be 60°C. In the present invention, the drying time may be 6 to 24 hours, and in a specific embodiment, may be 12 hours or 18 hours.

[0038] The present invention provides a method for preparing an antibacterial quantum dot material, comprising the following preparation steps: mixing a zinc source, an alkali source and a solvent, and performing microwave oscillation heating to obtain the antibacterial quantum dot material. The preparation method provided by the present invention uses microwave oscillation heating to synthesize the antibacterial quantum dot material. Due to the long-term microwave oscillation, the chemical reaction of the raw materials can be accelerated, so that the particles are more evenly dispersed, easier to form a sphere, and the particle size is more uniform, and the obtained zinc oxide quantum dots have a smaller particle size. It avoids the problems of mutual adsorption and excessive aggregation of zinc oxide during preparation by conventional methods, forming irregular shapes such as flakes or excessive particle size. At the same time, the zinc oxide quantum dots prepared based on this method have antibacterial and anti-drug resistance-reducing biological activity, and are an important substitute for reducing resistance and replacing resistance. The preparation method provided by the present invention has a short reaction time, few side reactions, simple operation, low cost, high efficiency, biological activity, and broad market conversion prospects. The yield of the antibacterial quantum dot material prepared by the preparation method provided by the present invention can reach 82.5%, and the purity is ≥98%.

[0039] The present invention also provides an antibacterial quantum dot material prepared by the preparation method described in the above technical solution, wherein the antibacterial quantum dot material is spherical quantum dots; the spherical quantum dots are arranged by single zinc oxide; the diameter of the spherical quantum dots is 80 to 150 nm, and in a specific embodiment, it can be 100 nm or 120 nm; the particle size of the single zinc oxide is 5 to 10 nm.

[0040] In the present invention, the mass percentage of zinc oxide is ≥98%, and in a specific embodiment, it can be 98%.

[0041] The antibacterial quantum dot material provided by the present invention is composed of single zinc oxide with a particle size of 5 to 10 nm, and the diameter of the spherical quantum dots is 80 to 150 nm. The particle size of the single zinc oxide in the antibacterial quantum dot material provided by the present invention is less than 10 nm, and the surface of each spherical quantum dot formed by agglomeration is loose, and the particles are evenly dispersed without obvious irregular shapes. In vitro antibacterial and drug resistance reduction tests show that it has significant antibacterial activity, and long-term use will not induce Escherichia coli and Staphylococcus aureus to develop drug resistance. The possible mechanism is that the zinc oxide quantum dots synthesized by this method have a smaller particle size and can better penetrate into the bacteria, thereby destroying the bacterial genetic material DNA, resulting in the inability of the bacteria to divide and propagate, and eventually die.

[0042] The present invention also provides the use of the antibacterial quantum dot material described in the above technical solution in the preparation of antibacterial drugs.

[0043] The antibacterial quantum dot material provided by the present invention has a small particle size, a large specific surface area, and a uniform particle size, and can load more antibacterial drugs. The antibacterial quantum dot material provided by the present application also has a good bactericidal and antibacterial effect, and will not induce bacteria or viruses to produce resistance. It can be widely used in the preparation of a variety of antibacterial drugs. Antibacterial experiments show that the zinc oxide quantum dot material has stable performance, not only has a good antibacterial effect, but also does not induce drug resistance. It has important application value and is expected to be newly applied in broad-spectrum antibacterial drugs, becoming an important substitute for reducing resistance and replacing resistance.

[0044] The technical solution of the present invention is further described below through specific implementation methods. Those skilled in the art should understand that the embodiments are only used to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0045] Example 1

[0046] According to anhydrous zinc chloride Zn 2+ The molar ratio of zinc chloride to hydroxide in the system is 1:2, anhydrous zinc chloride is dissolved in a 20wt% ethanol-water system to obtain a zinc chloride ethanol aqueous solution with a zinc chloride concentration of 0.1mol / L; potassium hydroxide is dissolved in deionized water to obtain a potassium hydroxide aqueous solution with a concentration of 0.5mol / L. 40mL of potassium hydroxide aqueous solution is quickly added to 100mL of zinc chloride ethanol aqueous solution, and microwave oscillation heating is performed while stirring at a speed of 500rpm, wherein the microwave oscillation heating temperature is 150°C, the power is 600W, and the time is 5min.

[0047] After the microwave oscillation heating is completed, the zinc oxide is separated by suction filtration, and the mixture is washed repeatedly three times with deionized water. The obtained zinc oxide is dried at 60° C. for 24 h to obtain the zinc oxide antibacterial quantum dot material.

[0048] The microscopic morphology of the zinc oxide antibacterial quantum dot material was detected by scanning electron microscopy. The test results are shown in Figure 1 .Depend on Figure 1 It can be seen that the obtained zinc oxide antibacterial quantum dot material is spherical, with a loose surface, uniform dispersion between particles, and no obvious irregular shape. The particle size of a single zinc oxide is 5 to 10 nm, and the particle size of the zinc oxide antibacterial quantum dot material is 80 to 150 nm.

[0049] The mass percentage of zinc oxide in the zinc oxide antibacterial quantum dot material detected by energy dispersive spectroscopy is 98%.

[0050] The zeta potential of the zinc oxide antibacterial quantum dot material was tested using a Malvern potential tester. After 0.1 g of the zinc oxide antibacterial quantum dot material was dispersed in deionized water for 15 min, the zeta potential was tested. The test results are shown in Figure 2 .Depend on Figure 2 It can be seen that the Zeta potential is 30.7 mV.

[0051] The antibacterial effect of the zinc oxide quantum dots obtained in Example 1 on Staphylococcus aureus and Escherichia coli was tested using the HG / T 3950-2007 standard. The test results showed that the zinc oxide quantum dots had a good antibacterial effect, with an antibacterial rate of 98%.

[0052] Example 2

[0053] The difference from Example 1 is that 60 mL of potassium hydroxide aqueous solution is quickly added to 100 mL of zinc chloride ethanol aqueous solution.

[0054] The characterization results of the zinc oxide quantum dots obtained in this example are as follows: the particle size of a single zinc oxide is 7 nm, the particle size of the zinc oxide antibacterial quantum dot material is 100 nm, the mass percentage of zinc oxide in the antibacterial quantum dot material is 95%, and the Zeta potential is 30.2 mV.

[0055] Comparative Example 1

[0056] The difference from Example 1 is that microwave oscillation heating is replaced by ordinary oil bath heating.

[0057] The test results show that the particle size of a single zinc oxide is 6-15 nm, the particle size of the zinc oxide antibacterial quantum dot material is 100-110 nm, the mass percentage of zinc oxide in the antibacterial quantum dot material is 87%, and the Zeta potential is 28.7 mV.

[0058] The Zeta potential of the zinc oxide antibacterial quantum dot material obtained in Comparative Example 1 is smaller than the Zeta potential of the zinc oxide antibacterial quantum dot material obtained in Examples 1 and 2, indicating that the zinc oxide antibacterial quantum dot material system prepared by microwave oscillation heating in the present invention is more stable.

[0059] Comparative Example 2

[0060] The difference from Example 1 is that the ordinary oil bath heating temperature is 70°C.

[0061] The test results show that the particle size of a single zinc oxide is 8-10 nm, the particle size of the zinc oxide antibacterial quantum dot material is 100-150 nm, the mass percentage of zinc oxide in the antibacterial quantum dot material is 85%, and the Zeta potential is 26.4 mV.

[0062] The zinc oxide mass percentage and Zeta potential of the zinc oxide antibacterial quantum dot material obtained in Comparative Example 2 are lower than the Zeta potential of the zinc oxide antibacterial quantum dot material obtained in Examples 1 and 2, indicating that the zinc oxide antibacterial quantum dots prepared by microwave oscillation heating in the present invention have a higher zinc oxide mass content and a more stable material system.

[0063] Comparative Example 3

[0064] The difference from Example 1 is that potassium hydroxide is replaced by sodium methoxide.

[0065] The test results are as follows: the particle size of a single zinc oxide is 30-50 nm, the particle size of the zinc oxide antibacterial quantum dot material is 150-200 nm, the mass percentage of zinc oxide in the antibacterial quantum dot material is 83%, and the Zeta potential is 28.3 mV.

[0066] The zinc oxide antibacterial quantum dot material obtained in Comparative Example 3 has a larger particle size, and the mass percentage of zinc oxide and the Zeta potential are both lower than the Zeta potential of the zinc oxide antibacterial quantum dot material obtained in Examples 1 to 2, indicating that the zinc oxide antibacterial quantum dot material prepared by microwave oscillation heating in the present invention has a smaller particle size, a higher mass content of zinc oxide, and a more stable system.

[0067] Test Example 1

[0068] Minimum inhibitory concentration (MIC) test:

[0069] Take 50 μL of well-preserved Escherichia coli (ATCC25922) and 50 μL of Staphylococcus aureus (ATCC25923), add them into 5 mL of LB liquid culture medium respectively, and culture them for 12 hours before use.

[0070] Dilute the cultured bacterial suspension to 10 6 ~10 8 CFU / mL.

[0071] The minimum inhibitory concentration test was performed using the 96-well plate method. 100 μL of LB liquid culture medium was added to each 96-well plate, and 100 μL of the zinc oxide quantum dot solution obtained in Example 1 was added to the first column of wells, and the double gradient dilution method was used to dilute step by step (so that the drug concentrations of wells 1-12 were: 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 3.91, 1.95, 0.98, 0.49, 0.24 μg / mL, respectively), and finally 100 μL of Escherichia coli and Staphylococcus aureus suspensions were added. At the same time, a blank control group (only LB culture medium) and a positive control (only bacterial solution) were set up separately, and cultured overnight in a 37°C incubator for 24 hours.

[0072] The OD of each well was observed by microplate reader. 600 The turbidity under the condition of MIC is determined. The test results are shown in Figure 3 .

[0073] Depend on Figure 3 It can be seen that after E. coli reached 31.25 μg / mL, OD 600 The value increased and was close to that of the positive control (POS with only bacterial solution added), indicating that the bacterial growth could not be inhibited. Therefore, the MIC of the zinc oxide antibacterial quantum dot material prepared in Example 1 against Escherichia coli was 31.25 μg / mL.

[0074] Staphylococcus aureus at 62.50 μg / mL, OD 600 The value increased and was close to that of the positive control (POS with only bacterial solution added), indicating that the bacterial growth could not be inhibited. Therefore, the MIC of the zinc oxide antibacterial quantum dot material prepared in Example 1 against Staphylococcus aureus was 62.50 μg / mL.

[0075] Test Example 2

[0076] Based on the clear sterile growth wells observed by naked eye according to the above MIC, i.e., wells 1-5 for E. coli and wells 1-4 for S. aureus, plate the wells to determine the MBC;

[0077] Divide the LB agar plate into several areas, then take 10 μL from the above wells and drop it into the plate grid. After the plate is dry, put it upside down in a 37℃ oven and culture it for 24 hours. The MBC plate test results of Escherichia coli and Staphylococcus aureus are shown in the figure. Figure 4 .

[0078] Depend on Figure 4 It can be seen that the MBC of the zinc oxide antibacterial quantum dot material prepared in Example 1 against Escherichia coli is 125 μg / mL, and the MBC against Staphylococcus aureus is 250 μg / mL.

[0079] Test Example 3

[0080] Induced resistance test

[0081] Escherichia coli ATCC25922 and Staphylococcus aureus ATCC25923 were used as model strains for drug resistance testing;

[0082] First, induce bacteria to develop drug resistance: Escherichia coli and Staphylococcus aureus were induced to develop drug resistance by repeatedly treating bacteria with sublethal doses (1 / 2MIC) of antimicrobial agents;

[0083] The zinc oxide quantum dots (treatment group) and vancomycin (control group) obtained in Example 1 were used to perform MIC determination of the drug-resistant bacteria induced previously for 20 days (MIC treat ), and the ratio calculated with the original MIC (MIC0) of the uninduced standard strain: MIC treat / MIC0; results of induced resistance test are shown in Figure 5 .

[0084] Depend on Figure 5 It can be seen that the MIC of the bacteria induced by the zinc oxide quantum dots prepared in Example 1 did not change substantially within 20 generations, indicating that the zinc oxide quantum dots did not induce drug resistance in the bacteria within 20 generations; the MIC of the bacteria induced by vancomycin in the control group increased by 4 times after 5 generations and increased by 16 times at the 20th generation, indicating that the long-term action of the broad-spectrum antibiotic vancomycin on bacteria will produce strong drug resistance.

[0085] Test Example 4

[0086] Clinical drug resistance and antibacterial effect

[0087] Multiple drug-resistant bacteria isolated from clinical sites: drug-resistant Staphylococcus aureus MRSA, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii were cultured in liquid culture medium until the logarithmic growth phase and adjusted to about 105 CFU / mL;

[0088] The experimental group was set up: the aqueous solution of the zinc oxide antibacterial quantum dot material prepared in Example 1 was added to the culture medium of different culture dishes, and the final concentrations were 1024, 512, 256, 128, 64, 32, 16, 8, 4, 2, 1, 0.5, and 0.25 μg / mL, respectively, and vancomycin; the blank group: only the culture medium was in the culture dish; the control group: drug-resistant Staphylococcus aureus MRSA, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii were inoculated in the culture medium of different culture dishes;

[0089] Each group of experiments was performed with 5 parallel samples. After the culture dish was placed in a 37°C incubator for 16 days, the MIC value was determined based on the clarification of the mixed solution in the well visible to the naked eye.

[0090] The MIC was tested according to the method described in Test Example 1. The test results are shown in Table 1:

[0091] Table 1 Minimum inhibitory concentration of zinc oxide antibacterial quantum dot materials obtained in Example 1 and Comparative Example 1 against clinical multiple drug-resistant strains

[0092]

[0093]

[0094] As can be seen from Table 1, the MIC of the zinc oxide antibacterial quantum dot material prepared in Example 1 against 5 clinically resistant Staphylococcus aureus is 32 μg / mL, the MIC of Klebsiella pneumoniae is 128-256 μg / mL, the MIC of Pseudomonas aeruginosa is 128-256 μg / mL, and the MIC of Acinetobacter baumannii is 64-128 μg / mL.

[0095] Compared with levofloxacin, the zinc oxide antibacterial quantum dots synthesized in Example 1 of the present invention have inhibitory effects on clinical strains resistant to levofloxacin and can exert a higher antibacterial effect at a lower concentration; compared with the nano zinc oxide synthesized by the conventional method described in Comparative Example 1, the antibacterial effect is better than that of nano zinc oxide.

[0096] In summary, zinc oxide quantum dots synthesized by microwave oscillation heating have highly efficient bactericidal and antibacterial effects. They not only have good antibacterial effects on clinical multidrug-resistant strains, but also do not cause bacterial resistance. They are an ideal alternative antibiotic material.

[0097] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A method for preparing an antibacterial quantum dot material, characterized in that: The method comprises the following preparation steps: The zinc source, the alkali source and the solvent are mixed and subjected to microwave oscillation heating to obtain the antibacterial quantum dot material.

2. The preparation method according to claim 1, characterized in that: The zinc source includes one or more of anhydrous zinc chloride, anhydrous zinc acetate and zinc acetate dihydrate.

3. The preparation method according to claim 1, characterized in that: The alkali source is an inorganic alkali source; the inorganic alkali source includes sodium hydroxide and / or potassium hydroxide.

4. The preparation method according to claim 1, characterized in that: The solvent includes an ethanol-water system; The concentration of ethanol in the ethanol-water system is 0 to 50 wt %.

5. The preparation method according to claim 1, characterized in that: The volume ratio of the amount of the zinc source to the solvent is 0.01 to 0.2 mol / L.

6. The preparation method according to claim 1, 2 or 4, characterized in that: In the mixed system, the molar ratio of the zinc source to the hydroxide is 1:1-3.

7. The preparation method according to claim 1, characterized in that: The microwave oscillation heating temperature is 120-180°C and the time is 5-30 minutes; The power of the microwave oscillation heating is 300-800W.

8. The antibacterial quantum dot material prepared by the preparation method according to any one of claims 1 to 7, characterized in that: The antibacterial quantum dot material is spherical quantum dots; the diameter of the spherical quantum dots is 80-150nm.

9. The antibacterial quantum dot material according to claim 8, characterized in that: The mass percentage of zinc oxide in the antibacterial quantum dot material is ≥98%.

10. Use of the antibacterial quantum dot material according to claim 8 or 9 in the preparation of antibacterial drugs.

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