Biological organic fertilizer for fennel planting and preparation method thereof

Abstract

The invention provides a bio-organic fertilizer for fennel planting and a preparation method thereof, and relates to the technical field of bio-organic fertilizers, the bio-organic fertilizer comprises the following raw materials: mushroom residues, starch residues, biochemical potassium fulvate, a trace element additive, potassium sulfate, seaweed meal, wood vinegar, straw powder, fishbone dust and a complex microbial inoculant, the strain is bacillus subtilis and bacillus amyloliquefaciens, and the strain is bacillus subtilis and bacillus amyloliquefaciens; the preparation method comprises three steps of activation of strains, preparation of the complex microbial inoculant and preparation of the organic fertilizer. According to the invention, compost fermentation is carried out by adding the composite compost microbial inoculum, the temperature can reach 68 DEG C during fermentation, the high-temperature period can be maintained for 14 days, innocent treatment is achieved, pathogenic bacteria and worm eggs in the raw materials are eliminated, and the composite compost microbial inoculum can play roles in inhibiting bacteria, preventing soil hardening, dissolving phosphorus, releasing potassium, fixing nitrogen and promoting rooting after being used.

Description

Technical Field

[0001] The invention relates to the technical field of bio-organic fertilizers, and in particular to a bio-organic fertilizer for fennel planting and a preparation method thereof. Background Art

[0002] Fennel is a thermophilic plant with certain requirements for soil environment. It is suitable for growing in a well-drained and fertile soil environment. In the process of fennel cultivation, organic fertilizer is an environmentally friendly and sustainable soil improvement and fertilizer source. It is rich in organic matter and nutrients required by various plants. It is widely used to improve soil structure and increase soil fertility. However, there are also some shortcomings in the actual application of organic fertilizer, which limits its effect in fennel cultivation.

[0003] Fennel has high requirements for soil, needs a loose and fertile soil environment, and needs to have good water retention and air permeability to avoid soil compaction. However, the decomposition speed of conventional organic fertilizer is slow, which easily leads to uneven release of nutrients, and is difficult to meet crop demand in time during the rapid growth period of fennel. Traditional organic fertilizer has limited effects in terms of antibacterial and disease prevention. If improperly handled during composting, it may carry harmful microorganisms such as pathogens and eggs, causing fennel to be susceptible to diseases. Meanwhile, organic fertilizer has limited effects in improving soil structure and preventing compaction, especially in soil of continuous planting, organic matter deficiency may not be able to keep the soil loose and breathable for a long time, affecting the growth and development of root system, so the present invention proposes a kind of fennel planting biological organic fertilizer and preparation method thereof to solve the problems existing in the prior art. Summary of the invention

[0004] In view of the above problems, the purpose of the present invention is to provide a biological organic fertilizer for fennel planting and a preparation method thereof, aiming to solve the problems of insufficient nutrient utilization, long production cycle, incomplete nutritional composition of organic fertilizer products, environmental pollution, etc. in the existing composting fermentation process.

[0005] To achieve the purpose of the present invention, the present invention is achieved through the following technical scheme: a biological organic fertilizer for fennel planting, comprising the following raw materials in parts by weight: 8-10 parts of mushroom residues, 3-4 parts of starch residues, 1-2 parts of biochemical potassium humate, 1-2 parts of trace element additives, 1-2 parts of potassium sulfate, 1-2 parts of seaweed powder, 1-2 parts of wood vinegar, 1-2 parts of straw powder, 1-2 parts of fish bone powder and composite bacterial agent, wherein the addition amount of the composite bacterial agent is 3-6% of the total amount of the materials, the composite bacterial agent is prepared by activating bacterial strains and inoculating them into a mixed bacterial fermentation medium, and the bacterial strains are Bacillus subtilis and Bacillus amyloliquefaciens.

[0006] A further improvement is that the mixed bacteria fermentation medium comprises the following components in parts by weight: 15-25 g / L soybean meal, 5-15 g / L corn flour, 0.5-1.5 g / L chloride salt, and the balance is water.

[0007] A further improvement is that the ratio of Bacillus subtilis to Bacillus amyloliquefaciens is 1:1.

[0008] A method for preparing a bio-organic fertilizer for fennel planting comprises the following steps:

[0009] Step 1: Activation of bacteria

[0010] Bacillus subtilis and Bacillus amyloliquefaciens were inoculated into LB medium at an inoculum size of 1%, respectively, and cultured under shaking for 16 hours, and then stored in a refrigerator at 4° C. to obtain activated Bacillus subtilis and Bacillus amyloliquefaciens;

[0011] Step 2: Preparation of composite bacterial agent

[0012] The activated Bacillus subtilis and Bacillus amyloliquefaciens are inoculated into a mixed fermentation medium at a ratio of 1:1, and cultured with shaking for 55 to 65 hours to obtain a composite bacterial agent;

[0013] Step 3: Preparation of organic fertilizer

[0014] The composite bacterial agent is mixed with mushroom residue, starch residue, potassium sulfate, seaweed powder, wood vinegar, straw powder, fish bone powder and biochemical potassium humate, and then water is added, and the mixture is piled into strips, which are turned over every 7 days. After fermentation for 28 days, the biological organic fertilizer is obtained.

[0015] A further improvement is that in step 1, the shaking culture conditions are: temperature 28°C to 30°C, and rotation speed 170r / min.

[0016] A further improvement is that in step 2, the shaking culture conditions are: temperature 28°C to 30°C, pH 7.0 to 7.2, and rotation speed 180 r / min.

[0017] A further improvement is that in step three, the initial addition amount of the composite bacterial agent is 2-4% of the total amount of the starting material, and the remaining composite bacterial agent is added after the internal temperature of the pile body drops to 40°C.

[0018] A further improvement is that in step three, after adding water, the water content of the compost material is adjusted to 60%.

[0019] The beneficial effects of the present invention are as follows: the present invention efficiently treats and utilizes agricultural waste by adding a composite bacterial agent, and the temperature of the compost can reach 68°C during compost fermentation, and the high temperature period can be maintained for up to 14 days, thereby achieving the complete killing of pathogens and insect eggs and achieving the goal of harmless treatment. At the same time, during the fermentation process, the composite bacterial agent can decompose organic matter, release nitrogen, phosphorus, potassium and other nutrients that are easily absorbed by plants, and further improve the nutrient utilization efficiency of fertilizers through the phosphorus dissolving, potassium releasing and nitrogen fixing functions of the bacterial agent. At the same time, the bacterial agent also has the effects of antibacterial and rooting and growth promotion, which can effectively enhance the disease resistance and root development ability of fennel.

[0020] The present invention increases the organic matter content of the soil, improves the soil aggregate structure, and improves the water storage and drainage capacity of the soil by adding materials such as mushroom residues, straw powder, and biochemical potassium humate, thereby ensuring that the root system of fennel is in a good growth environment. The present invention further provides nitrogen, phosphorus, potassium, and medium and trace elements required by fennel through the synergistic effect of materials such as potassium sulfate, fish bone powder, trace element additives, and seaweed powder, thereby providing a more comprehensive nutrient supply, meeting the needs of fennel at different stages from the seedling stage to the growth stage, and improving the crop quality and commercial value. The composite bacterial agent can improve the nutrient effectiveness in the soil through the effects of dissolving phosphorus and releasing potassium, fixing nitrogen, and the like, and is combined with wood vinegar to further inhibit the reproduction of pathogens, reduce soil-borne diseases such as root rot and wilt that are prone to fennel, and enhance the disease and stress resistance of fennel. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic flow chart of the preparation steps of the present invention. DETAILED DESCRIPTION

[0022] In order to deepen the understanding of the present invention, the present invention will be further described in detail below in conjunction with examples. The examples are only used to explain the present invention and do not constitute a limitation on the protection scope of the present invention.

[0023] The sources of the materials in the following examples are all commercially purchased. LB medium is a meat extract peptone medium, a medium widely used for culturing bacteria, and the preparation method is as follows: take 10g / L of tryptone, 5g / L of yeast extract and 10g / L of NaCl, adjust the pH to 7.0, and dilute to 1L with deionized water. Composite bacterial agent refers to the composite strain used together with the culture medium. The following Bacillus subtilis agent was purchased from Jinan Jinyuyuan Biotechnology Co., Ltd., and the number of viable bacteria was ≥100 billion / g. The following Bacillus amyloliquefaciens agent was purchased from Beihai Qiangxing Biotechnology Co., Ltd.

[0024] The reduction and resource utilization of agricultural organic solid waste is an effective way to reduce environmental pollution. At present, the main way to realize the resource utilization of agricultural waste is composting. However, the composting process often encounters problems such as low fermentation temperature, long fermentation time, incomplete killing of pathogens and eggs, insufficient fermentation and loss of nutrients. Therefore, promoting compost maturity and shortening the composting fermentation cycle are the key to improving composting quality and efficiency. Mushroom residue and starch residue are the main agricultural wastes in the Hexi region of Gansu. They are easy to deteriorate and difficult to preserve due to large output.

[0025] Therefore, based on the following embodiments, the present invention can achieve the harmless treatment and reuse of agricultural waste. No new waste liquid and solid waste are generated during the treatment process. The types of auxiliary materials added are few, the cost is low, and no new substances that may affect the environment are introduced. The overall treatment difficulty is low, the efficiency is high, and it is environmentally friendly.

[0026] Embodiment 1

[0027] according to Figure 1 As shown, this embodiment proposes a biological organic fertilizer for fennel planting, comprising the following raw materials in parts by weight: 9 parts of mushroom residue, 3 parts of starch residue, 2 parts of biochemical potassium humate, 1 part of trace element additive, 1 part of potassium sulfate, 1 part of seaweed powder, 1 part of wood vinegar, 1 part of straw powder, 1 fish bone powder and composite bacterial agent, the trace element additive is magnesium sulfate, the addition amount of the composite bacterial agent is 4% of the total amount of the material, the composite bacterial agent is obtained by inoculating activated strains in a mixed bacterial fermentation medium, the strains are Bacillus subtilis and Bacillus amyloliquefaciens, wherein the mixed bacterial fermentation medium comprises the following components in parts by weight: 20g / L soybean meal, 13g / L corn flour, 1g / L chloride, and the remainder is water, wherein the ratio of Bacillus subtilis to Bacillus amyloliquefaciens is 1:1.

[0028] A method for preparing a bio-organic fertilizer for fennel planting comprises the following steps:

[0029] Step 1: Activation of bacteria

[0030] Under a sterile operating environment, Bacillus subtilis and Bacillus amyloliquefaciens are inoculated in a sterilized and cooled LB medium at an inoculum amount of 1%, and the LB medium is placed in a triangular flask. The seed strains of Bacillus subtilis and Bacillus amyloliquefaciens are taken from the preserved strains, respectively. The inoculated triangular flask is placed in a constant temperature shaking incubator, and the following culture conditions are set: temperature 29°C (optimal growth temperature range of the strain, ensuring high metabolic activity), speed 170r / min (moderate shaking frequency helps oxygen supply and enhances bacterial proliferation). Then, shake culture for 16 hours, and then put it in a 4°C refrigerator to obtain activated Bacillus subtilis and Bacillus amyloliquefaciens. The purpose of activation is to make the bacterial body enter the logarithmic growth phase through cultivation, with active metabolism and fast proliferation rate, laying the foundation for subsequent mixed bacteria fermentation.

[0031] Step 2: Preparation of composite bacterial agent

[0032] The activated Bacillus subtilis and Bacillus amyloliquefaciens were inoculated in a mixed fermentation medium at a ratio of 1:1. The mixed fermentation medium had been sterilized and cooled. Furthermore, a constant temperature shaking incubator was used to set the following culture conditions: temperature 29°C, pH 7.1 (suitable for the growth environment of Bacillus subtilis and Bacillus amyloliquefaciens), and speed 180r / min. The composite bacterial agent was obtained by shaking and culturing for 60 hours. Among them, Bacillus subtilis can produce antimicrobial peptides, inhibit harmful microorganisms in compost raw materials, decompose complex organic matter in compost, and accelerate the fermentation process. Bacillus amyloliquefaciens can decompose insoluble phosphorus and potassium in the soil, release effective phosphorus and potassium for plant absorption, and secrete growth-promoting substances to improve the development of crop root systems. Therefore, the synergistic effect of Bacillus subtilis and Bacillus amyloliquefaciens can significantly improve the compost fermentation efficiency and optimize the quality of fertilizers.

[0033] Step 3: Preparation of organic fertilizer

[0034] The compound bacterial agent is mixed with mushroom residue (rich in organic matter and fiber, as the main source of organic matter), starch residue (provides a fermentable carbon source and energy for microorganisms), potassium sulfate (provides potassium for the soil, promotes photosynthesis and the healthy growth of stems and leaves), seaweed powder (rich in a variety of trace elements, balances nutrients in the soil, and enhances the comprehensive fertilizer effect of fertilizers), wood vinegar (improves the soil microenvironment, inhibits the growth of harmful microorganisms in the soil, and prevents compaction), straw powder (loosens the soil, improves air permeability and water permeability), fish bone powder (improves The compost is mixed with natural phosphorus source and organic matter to promote root and overall plant growth) and biochemical potassium humate (to improve soil structure and promote plant absorption of nutrients), and then water is added to adjust the moisture content of the compost material to 60%. The compost is piled into strips. The shape of the strips is conducive to air circulation to avoid oxygen deficiency during the composting fermentation process. The compost is turned over every 7 days. After 28 days of fermentation, the biological organic fertilizer is obtained. The initial addition amount of the composite bacterial agent is 3% of the total amount of the starting material. After the internal temperature of the pile drops to 40°C, the remaining composite bacterial agent is added. Specifically, in the early stage of fermentation, the temperature of the pile rises rapidly and enters a high temperature stage (55-68°C). The high temperature period can last for 14 days. During this stage, pathogens, insect eggs and weed seeds are killed. When the internal temperature of the pile drops to 40°C, the remaining composite bacterial agent is added, which can effectively decompose organic matter and increase the nutrient content of the fertilizer.

[0035] Embodiment 2

[0036] according to Figure 1As shown, this embodiment proposes a biological organic fertilizer for fennel planting, including the following raw materials by weight: 8 parts of mushroom residue, 4 parts of starch residue, 2 parts of biochemical potassium humate, 2 parts of trace element additives, 2 parts of potassium sulfate, 2 parts of seaweed powder, 2 parts of wood vinegar, 2 parts of straw powder, 2 parts of fish bone powder and composite bacterial agent, the trace element additive is magnesium sulfate, the composite bacterial agent is added in an amount of 6% of the total amount of the material, and the composite bacterial agent is obtained by inoculating activated strains in a mixed bacterial fermentation medium, the strains are Bacillus subtilis and Bacillus amyloliquefaciens, wherein the mixed bacterial fermentation medium includes the following components by weight: 25g / L soybean meal, 15g / L corn flour, 1.5g / L chloride, the remainder is water, and the mixed bacterial fermentation medium is based on 1L. The ratio of Bacillus subtilis to Bacillus amyloliquefaciens is 1:1.

[0037] A method for preparing a bio-organic fertilizer for fennel planting comprises the following steps:

[0038] Step 1: Activation of bacteria

[0039] Under a sterile operating environment, Bacillus subtilis and Bacillus amyloliquefaciens are inoculated in a sterilized and cooled LB medium at an inoculum amount of 1%, and the LB medium is placed in a triangular flask. The seed strains of Bacillus subtilis and Bacillus amyloliquefaciens are taken from the preserved strains, respectively. The inoculated triangular flask is placed in a constant temperature shaking incubator, and the following culture conditions are set: temperature 28 (optimal growth temperature range of the strain, ensuring high metabolic activity), speed 170r / min (moderate shaking frequency helps oxygen supply and enhances bacterial proliferation). Then, shake culture for 16 hours, and then put it in a 4°C refrigerator to obtain activated Bacillus subtilis and Bacillus amyloliquefaciens. The purpose of activation is to make the bacterial body enter the logarithmic growth phase through cultivation, with active metabolism and fast proliferation rate, laying the foundation for subsequent mixed bacteria fermentation.

[0040] Step 2: Preparation of composite bacterial agent

[0041] The activated Bacillus subtilis and Bacillus amyloliquefaciens were inoculated in a mixed fermentation medium at a ratio of 1:1. The mixed fermentation medium had been sterilized and cooled. Furthermore, a constant temperature shaking incubator was used to set the following culture conditions: temperature 28°C, pH 7.0 (suitable for the growth environment of Bacillus subtilis and Bacillus amyloliquefaciens), and speed 180r / min. The composite bacterial agent was obtained after 55 hours of shaking culture. Among them, Bacillus subtilis can produce antimicrobial peptides, inhibit harmful microorganisms in compost raw materials, decompose complex organic matter in compost, and accelerate the fermentation process. Bacillus amyloliquefaciens can decompose insoluble phosphorus and potassium in the soil, release effective phosphorus and potassium for plant absorption, and secrete growth-promoting substances to improve the development of crop root systems. Therefore, the synergistic effect of Bacillus subtilis and Bacillus amyloliquefaciens can significantly improve the compost fermentation efficiency and optimize the quality of fertilizers.

[0042] Step 3: Preparation of organic fertilizer

[0043] The compound bacterial agent is mixed with mushroom residue (rich in organic matter and fiber, as the main source of organic matter), starch residue (provides a fermentable carbon source and energy for microorganisms), potassium sulfate (provides potassium for the soil, promotes photosynthesis and the healthy growth of stems and leaves), seaweed powder (rich in a variety of trace elements, balances nutrients in the soil, and enhances the comprehensive fertilizer effect of fertilizers), wood vinegar (improves the soil microenvironment, inhibits the growth of harmful microorganisms in the soil, and prevents compaction), straw powder (loosens the soil, improves air permeability and water permeability), fish bone powder (improves The compost is mixed with natural phosphorus source and organic matter to promote root system and overall plant growth) and biochemical potassium humate (to improve soil structure and promote plant absorption of nutrients), and then water is added to adjust the moisture content of the compost material to 60%, and the compost is piled into strips. The strip shape is conducive to air circulation to avoid oxygen deficiency during the compost fermentation process. The compost is turned over every 7 days. After 28 days of fermentation, the biological organic fertilizer is obtained. The initial addition amount of the composite bacterial agent is 2% of the total amount of the starting material. After the internal temperature of the pile drops to 40°C, the remaining composite bacterial agent is added. Specifically, in the early stage of fermentation, the temperature of the pile rises rapidly and enters a high temperature stage (55-68°C). The high temperature period can last for 14 days. During this stage, pathogens, insect eggs and weed seeds are killed. When the internal temperature of the pile drops to 40°C, the remaining composite bacterial agent is added, which can effectively decompose organic matter and increase the nutrient content of the fertilizer.

[0044] Embodiment 3

[0045] according to Figure 1 As shown, this embodiment provides a biological organic fertilizer for fennel planting, including the following raw materials in parts by weight: 10 parts of mushroom residue, 3 parts of starch residue, 1 part of biochemical potassium humate, 1 part of trace element additive, 1 part of potassium sulfate, 1 part of seaweed powder, 1 part of wood vinegar, 1 part of straw powder, 1 part of fish bone powder and composite bacterial agent, the trace element additive is magnesium sulfate, the addition amount of the composite bacterial agent is 3% of the total amount of the material, the composite bacterial agent is obtained by inoculating activated strains in a mixed bacterial fermentation medium, the strains are Bacillus subtilis and Bacillus amyloliquefaciens, wherein the mixed bacterial fermentation medium comprises the following components in parts by weight: 15g / L soybean meal, 5g / L corn flour, 0.5g / L chloride, and the balance is water, wherein the ratio of Bacillus subtilis to Bacillus amyloliquefaciens is 1:1.

[0046] A method for preparing a bio-organic fertilizer for fennel planting comprises the following steps:

[0047] Step 1: Activation of bacteria

[0048] Under a sterile operating environment, Bacillus subtilis and Bacillus amyloliquefaciens are inoculated in a sterilized and cooled LB medium at an inoculation amount of 1%, and the LB medium is placed in a triangular flask. The seed strains of Bacillus subtilis and Bacillus amyloliquefaciens are taken from the preserved strains, respectively. The inoculated triangular flask is placed in a constant temperature shaking incubator, and the following culture conditions are set: temperature 30°C (optimal growth temperature range of the strain, ensuring high metabolic activity), speed 170r / min (moderate shaking frequency helps oxygen supply and enhances bacterial proliferation). Then, shake culture for 16 hours, and then put it in a 4°C refrigerator to obtain activated Bacillus subtilis and Bacillus amyloliquefaciens. The purpose of activation is to make the bacterial body enter the logarithmic growth phase through cultivation, with active metabolism and fast proliferation rate, laying the foundation for subsequent mixed bacteria fermentation.

[0049] Step 2: Preparation of composite bacterial agent

[0050] The activated Bacillus subtilis and Bacillus amyloliquefaciens were inoculated in a mixed fermentation medium at a ratio of 1:1. The mixed fermentation medium had been sterilized and cooled. Furthermore, a constant temperature shaking incubator was used to set the following culture conditions: temperature 30°C, pH 7.2 (suitable for the growth environment of Bacillus subtilis and Bacillus amyloliquefaciens), and speed 180r / min. The composite bacterial agent was obtained by shaking and culturing for 65 hours. Among them, Bacillus subtilis can produce antimicrobial peptides, inhibit harmful microorganisms in compost raw materials, decompose complex organic matter in compost, and accelerate the fermentation process. Bacillus amyloliquefaciens can decompose insoluble phosphorus and potassium in the soil, release effective phosphorus and potassium for plant absorption, and secrete growth-promoting substances to improve the development of crop root systems. Therefore, the synergistic effect of Bacillus subtilis and Bacillus amyloliquefaciens can significantly improve the compost fermentation efficiency and optimize the quality of fertilizers.

[0051] Step 3: Preparation of organic fertilizer

[0052] The compound bacterial agent is mixed with mushroom residue (rich in organic matter and fiber, as the main source of organic matter), starch residue (provides a fermentable carbon source and energy for microorganisms), potassium sulfate (provides potassium for the soil, promotes photosynthesis and the healthy growth of stems and leaves), seaweed powder (rich in a variety of trace elements, balances nutrients in the soil, and enhances the comprehensive fertilizer effect of fertilizers), wood vinegar (improves the soil microenvironment, inhibits the growth of harmful microorganisms in the soil, and prevents compaction), straw powder (loosens the soil, improves air permeability and water permeability), fish bone powder (improves The compost is mixed with natural phosphorus source and organic matter to promote root and overall plant growth) and biochemical potassium humate (to improve soil structure and promote plant absorption of nutrients), and then water is added to adjust the moisture content of the compost material to 60%. The compost is piled into strips. The shape of the strips is conducive to air circulation to avoid oxygen deficiency during the composting fermentation process. The compost is turned over every 7 days. After 28 days of fermentation, the biological organic fertilizer is obtained. The initial addition amount of the composite bacterial agent is 4% of the total amount of the starting material. After the internal temperature of the pile drops to 40°C, the remaining composite bacterial agent is added. Specifically, in the early stage of fermentation, the temperature of the pile rises rapidly and enters a high temperature stage (55-68°C). The high temperature period can last for 14 days. During this stage, pathogens, insect eggs and weed seeds are killed. When the internal temperature of the pile drops to 40°C, the remaining composite bacterial agent is added, which can effectively decompose organic matter and increase the nutrient content of the fertilizer.

[0053] Fennel has high requirements for soil during the planting process. The advantages and disadvantages of the soil will directly affect its growth and yield. When the soil organic matter content is low, the soil microbial activity is insufficient and the nutrient supply capacity is poor, which affects the rapid growth and yield of fennel. Poor soil permeability and drainage will cause root hypoxia and rot, seriously affecting the growth of fennel. Therefore, during the planting process of fennel, soil rich in humus is needed, which can provide fennel with a stable nutrient supply and promote root growth. At the same time, it needs to be loose and breathable.

[0054] Therefore, the present invention is aimed at the soil environment required in the process of fennel planting. Materials such as mushroom residues, straw powder and potassium humate can effectively increase the organic matter content of the soil, thereby improving the soil structure, preventing hardening, and improving air permeability and water retention. Correspondingly, materials such as starch residues, potassium sulfate, and fish bone powder can provide essential elements such as nitrogen, phosphorus, potassium, and magnesium to ensure the comprehensive nutrition required for fennel at each growth stage. Composite bacterial agents and wood vinegar can then effectively inhibit the reproduction of harmful bacteria, enhance the disease resistance of fennel, reduce the occurrence of soil-borne diseases, and improve the disease resistance of fennel. Seaweed powder is combined with composite bacterial agents to promote the reproduction of beneficial microorganisms, improve the soil microecological environment, and improve nutrient conversion efficiency. Therefore, the present invention can not only improve the soil and improve fertilizer efficiency, but also meet the requirements of fennel for nutrients and growth environment, and greatly improve yield and quality.

[0055] The application of the biological organic fertilizer provided in Example 2 in fennel cultivation has been verified by field trials organized by the Jinchang Municipal Agriculture and Rural Affairs Bureau, and has the following beneficial effects: by adding a composite composting agent for composting fermentation, the temperature of the fermentation itself can reach 68°C, and the high temperature period can be maintained for 14 days, all of which achieve harmless treatment, eliminate pathogens and insect eggs in the raw materials, and can inhibit bacteria, prevent soil compaction, dissolve phosphorus and release potassium, fix nitrogen, and help rooting after use.

[0056] The quality is judged by testing the ingredients in the biological organic fertilizer, wherein the number of effective live bacteria in the biological organic fertilizer is greater than 20 million / g, the mass fraction of organic matter (on a drying basis) is greater than 45%, the mass fraction of water (fresh sample) is less than 30%, the mortality rate of ascaris eggs reaches 100%, the number of fecal coliform bacteria is less than the plant height and stem thickness per gram respectively, no total arsenic, mercury, lead, cadmium and chromium are detected, the pH is 6-8.5, and the biological organic fertilizer has reached the national standard NY884-2012.

[0057] With bio-organic fertilizer as main material, with chemical fertilizers such as diammonium phosphate and urea, four treatments and three repetitions are adopted, namely the first treatment (no fertilization, blank control), the second treatment (conventional fertilization), the third treatment (matrix fertilization) and the fourth treatment (the bio-organic fertilizer treatment of embodiment one), the yield, fertilizer application, cost, yield increase rate and economic benefit in the monitoring planting process are monitored. The result shows that after the base manure is applied with the bio-organic fertilizer, the fennel leaf color is dark green and commercial property is good. Its plant height and stem thickness are improved to varying degrees. When measured at harvest, the fourth treatment (bio-organic fertilizer treatment) is compared with the second treatment (conventional fertilization), and the fennel plant height and stem thickness are increased by 16cm and 1.9mm respectively; the fourth treatment (bio-organic fertilizer treatment) is compared with the second treatment (conventional fertilization), and the number of cave plants, the number of branches and the number of per mu of caves are 6.2, 6.6 and 38 respectively, and the base manure application of the bio-organic fertilizer relative to conventional fertilization plant height and stem thickness increases by 7cm and 1mm respectively, and the number of cave plants and the number of branches increase to varying degrees. After applying the bio-organic fertilizer, the yield of the fourth treatment (bio-organic fertilizer treatment) was significantly improved, and the yield per hectare reached 139.8kg, which was 8.6kg higher than the second treatment (conventional fertilization), with an increase rate of 6.6%, and a significant increase in yield. It was 5.67kg higher than the third treatment (matrix fertilization), with an increase rate of 4.32%, and a significant increase in yield. The input-output ratio of the test fertilizer was 1:5.0, with significant production effects and economic effects. Among them, the matrix fertilization used bio-organic fertilizer produced by Gansu Yasheng Jinchangsheng Agricultural Bio-Organic Fertilizer Co., Ltd. Conventional fertilization does not use bio-organic fertilizers, but only inorganic fertilizers.

[0058] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the framework and scope of application of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A bio-organic fertilizer for fennel planting, characterized in that: The invention comprises the following raw materials in parts by weight: 8 to 10 parts of mushroom residues, 3 to 4 parts of starch residues, 1 to 2 parts of biochemical potassium humate, 1 to 2 parts of trace element additives, 1 to 2 parts of potassium sulfate, 1 to 2 parts of seaweed powder, 1 to 2 parts of wood vinegar, 1 to 2 parts of straw powder, 1 to 2 parts of fish bone powder and composite bacterial agent, wherein the addition amount of the composite bacterial agent is 3 to 6% of the total amount of the materials, the composite bacterial agent is prepared by activating bacterial strains and inoculating them into a mixed bacterial fermentation medium, the bacterial strains are Bacillus subtilis and Bacillus amyloliquefaciens, and the trace element additive is magnesium sulfate.

2. A biological organic fertilizer for fennel planting, characterized in that: The mixed bacteria fermentation medium comprises the following components in parts by weight: 15-25 g / L soybean meal, 5-15 g / L corn flour, 0.5-1.5 g / L chloride salt, and the balance is water.

3. A biological organic fertilizer for fennel planting, characterized in that: The ratio of Bacillus subtilis to Bacillus amyloliquefaciens is 1:

1.

4. A method for preparing a bio-organic fertilizer for fennel planting according to claim 1, characterized in that: The following steps are involved: Step 1: Activation of bacteria Bacillus subtilis and Bacillus amyloliquefaciens were inoculated into LB medium at an inoculum size of 1%, respectively, and cultured under shaking for 16 hours, and then stored in a refrigerator at 4° C. to obtain activated Bacillus subtilis and Bacillus amyloliquefaciens; Step 2: Preparation of composite bacterial agent The activated Bacillus subtilis and Bacillus amyloliquefaciens are inoculated into a mixed fermentation medium at a ratio of 1:1, and cultured with shaking for 55 to 65 hours to obtain a composite bacterial agent; Step 3: Preparation of organic fertilizer The composite bacterial agent is mixed with mushroom residue, starch residue, potassium sulfate, seaweed powder, wood vinegar, straw powder, fish bone powder and biochemical potassium humate, and then water is added, and the mixture is piled into strips, which are turned over every 7 days. After fermentation for 28 days, the biological organic fertilizer is obtained.

5. The method for preparing a bio-organic fertilizer for fennel planting according to claim 4, wherein: In the step 1, the shaking culture conditions are: temperature 28°C to 30°C, and rotation speed 170r / min.

6. The method for preparing a bio-organic fertilizer for fennel planting according to claim 4, characterized in that: In the step 2, the shaking culture conditions are: temperature 28°C to 30°C, pH 7.0 to 7.2, and rotation speed 180 r / min.

7. The method for preparing a bio-organic fertilizer for fennel planting according to claim 4, wherein: In the step 3, the initial addition amount of the composite bacterial agent is 2-4% of the total amount of the starting materials, and the remaining composite bacterial agent is added after the internal temperature of the pile body drops to 40°C.

8. The method for preparing a bio-organic fertilizer for fennel planting according to claim 4, characterized in that: In the step 3, after adding water, the water content of the compost material is adjusted to 60%.

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