Poria cocos-containing composition as well as preparation process and application thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine extraction, and provides a poria cocos-containing composition as well as a preparation process and application thereof. The extraction process of the pachymaran extract comprises the following steps: A1, mixing poria cocos powder with a first solvent, extracting, and separating to obtain a first filtrate; a2, mixing the first filtered substance with a second solvent, and leaching to obtain a first leaching solution; a3, mixing the first leaching solution with a third solvent, separating to obtain a second leaching solution, adjusting the pH value of the second leaching solution to be neutral, and separating to obtain a second filtrate; a4, dialyzing, centrifuging and drying the second filtrate to obtain a pachymaran extract; the first solvent is a glycerin monostearate solution and petroleum ether; the second solvent comprises a sodium hydroxide solution; the third solvent is chloroform and n-butyl alcohol. According to the technical scheme, the problem that the extraction rate is low when the pachymaran extract is extracted from poria cocos in the prior art is solved.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine extraction, and in particular to a composition containing Poria cocos and a preparation process and application thereof. Background Art

[0002] Poria polysaccharide, as the active ingredient of the traditional Chinese medicine Poria, is mainly composed of β-(1→3)-D-glucan with a small amount of β-(1→6) branched chains. This polysaccharide exhibits multiple biological activities such as promoting cell division, complement activation, mutagenesis, anti-tumor and anti-cancer, and can significantly enhance immune function. Therefore, it has been widely used in the fields of medical care and food. At the same time, it can also effectively cooperate with other traditional Chinese medicine components to show effects such as enhancing the body's immunity, anti-inflammatory and antiviral effects, and has a broader application prospect.

[0003] Poria cocos polysaccharide is extracted and purified from the fruiting body of the Polyporaceae fungus. Traditional preparation methods include water extraction and alcohol precipitation, dilute alkali extraction, fermentation and alcohol precipitation, microwave-assisted extraction, etc. Among them, water extraction and alcohol precipitation and dilute alkali extraction are the most commonly used, but there are defects such as long extraction time and low extraction rate. Some new extraction technologies developed in recent years, such as supercritical fluid extraction and high-voltage pulsed electric field method, can extract polysaccharide components efficiently and gently, but due to the expensive equipment, their actual utilization rate is not high. Therefore, a method for extracting Poria cocos polysaccharide extract is proposed, which can improve the extraction rate of Poria cocos polysaccharide extract, which plays an important role in improving the pharmacological effects of Chinese medicine compositions containing Poria cocos polysaccharide extract. Summary of the invention

[0004] The invention provides a composition containing Poria cocos and a preparation process and application thereof, which solves the problem of low extraction rate when extracting Poria cocos polysaccharide extract from Poria cocos in the related art.

[0005] The technical solution of the present invention is as follows: The present invention provides an extraction process of a tuckahoe polysaccharide extract, comprising the following steps: A1, mixing the Poria powder with a first solvent, extracting, and separating to obtain a first filtrate; A2, mixing the first filtrate with a second solvent, extracting, and obtaining a first extract; A3, mixing the first extract and the third solvent, separating, obtaining a second extract, adjusting the pH value of the second extract to neutral, separating, and obtaining a second filtrate; A4, dialyzing, centrifuging and drying the second filtrate to obtain a pachymaran extract; The first solvent is glyceryl monostearate solution and petroleum ether; The second solvent comprises sodium hydroxide solution; The third solvent is chloroform and n-butanol.

[0006] As a further technical solution, the volume ratio of the glyceryl monostearate solution to petroleum ether is 2:3-8.

[0007] When the volume ratio of monostearate glyceryl solution to petroleum ether is 2:3~8, the extraction rate of Poria cocos polysaccharide extract can be further improved.

[0008] As a further technical solution, the second solvent also includes a sodium sulfite aqueous solution and a polyethylene glycol aqueous solution.

[0009] In the present invention, when the second solvent also includes a sodium sulfite aqueous solution and a polyethylene glycol aqueous solution, the combined use of the three solutions of sodium hydroxide solution, sodium sulfite aqueous solution and polyethylene glycol aqueous solution can provide a stable dissolution environment for the Poria cocos polysaccharide extract, while also helping the Poria cocos polysaccharide extract to dissolve completely and quickly, thereby further improving the extraction rate of the Poria cocos polysaccharide extract.

[0010] As a further technical solution, the volume ratio of the sum of the volumes of the sodium sulfite aqueous solution and the polyethylene glycol aqueous solution to the volume of the sodium hydroxide solution is 1:4; The volume ratio of the sodium sulfite aqueous solution to the polyethylene glycol aqueous solution is 0.5:0.5-1.5.

[0011] When the volume ratio of the sodium sulfite aqueous solution and the polyethylene glycol aqueous solution to the sodium hydroxide solution is 1:4, and the volume ratio of the sodium sulfite aqueous solution and the polyethylene glycol aqueous solution is 0.5:0.5-1.5, the extraction rate of the Poria cocos polysaccharide extract can be further improved.

[0012] As a further technical solution, the concentration of the sodium hydroxide solution is 0.4-0.6 mol / L; The glyceryl monostearate solution is obtained by dissolving glyceryl monostearate in anhydrous ethanol; The mass fraction of glyceryl monostearate in the glyceryl monostearate solution is 5% to 10%.

[0013] As a further technical solution, the concentration of the sodium sulfite aqueous solution is 0.2-0.3 mol / L.

[0014] As a further technical solution, the concentration of the polyethylene glycol aqueous solution is 0.05-0.15 mol / L.

[0015] As a further technical solution, the weight average molecular weight of the polyethylene glycol is 400-800.

[0016] As a further technical solution, the mass volume ratio of the Poria powder and the first solvent is 10g:120~160mL; The mass volume ratio of the Poria powder and the second solvent is 10g:400-600mL; The mass volume ratio of the Poria powder and the third solvent is 10g:80-120mL; The volume ratio of chloroform to n-butanol is 4-5:1.

[0017] As a further technical solution, in step A1, the extraction temperature is 40-50°C and the extraction time is 2-3h.

[0018] As a further technical solution, in step A2, the extraction temperature is 4-6°C and the extraction time is 1.5-2.5h.

[0019] As a further technical solution, in step A3, the mixing time is 30 to 50 minutes.

[0020] As a further technical solution, in step A3, when adjusting the second extract to neutral, a hydrochloric acid solution with a concentration of 0.4-0.6 mol / L is used.

[0021] The present invention also provides a composition containing Poria cocos, comprising the Poria cocos polysaccharide extract obtained by extracting the Poria cocos polysaccharide extract using the extraction process.

[0022] As a further technical solution, the composition containing Poria cocos comprises the following components in parts by weight: 20-25 parts of Poria cocos polysaccharide extract, 10-15 parts of Astragalus, 8-12 parts of Chuanxiong, 10-14 parts of Bletilla striata, and 15-20 parts of Licorice.

[0023] The present invention also provides a preparation process of the composition containing Poria cocos, comprising the following steps: S1. Weigh astragalus, chuanxiong, bletilla striata and liquorice, crush and sieve to obtain mixed Chinese medicine powder; S2, mixing the mixed Chinese medicine powder with water, heating and boiling for the first time, filtering to obtain a first filtrate and a filter residue, mixing the filter residue with water, heating and boiling for the second time, filtering to obtain a second filtrate, combining the first filtrate and the second filtrate, concentrating, and drying to obtain a mixed Chinese medicine extract; S3, mixing the mixed Chinese medicinal extract and the Poria cocos polysaccharide extract, adding the mixture into water, mixing evenly, and boiling to obtain the Poria cocos-containing composition.

[0024] As a further technical solution, in step S2, when the mixed Chinese medicine powder is mixed with water, the amount of water added is 8 to 10 times the mass of the mixed Chinese medicine powder; when the filter residue is mixed with water, the amount of water added is 5 to 6 times the mass of the filter residue.

[0025] As a further technical solution, in step S2, the first heating and boiling and the second heating and boiling are each independently performed for 1 to 2 hours.

[0026] As a further technical solution, in step S3, the amount of water added is 8 to 10 times the mass of the mixed Chinese medicine extract; during the decoction, the temperature is 50 to 60° C. and the time is 2 to 3 hours.

[0027] The present invention also proposes the application of the Poria cocos-containing composition or the Poria cocos-containing composition prepared by the preparation process in immunoregulation.

[0028] In the present invention, the Poria cocos polysaccharide extract can be used in combination with other traditional Chinese medicine components, and the prepared composition has a good immunoregulatory effect.

[0029] The working principle and beneficial effects of the present invention are: In the present invention, the extraction process of the Poria cocos polysaccharide extract adopts three different solvents, wherein the first solvent is a monostearate glyceryl solution and petroleum ether, and the monostearate glyceryl solution and the petroleum ether have a synergistic effect. Under the joint action of the two, the impurity components in the Poria cocos powder can be better dissolved, thereby assisting the initial release of the Poria cocos polysaccharide extract, and in conjunction with the subsequent extraction process, the Poria cocos polysaccharide extract components can be efficiently extracted from the Poria cocos powder raw material, thereby significantly improving the extraction rate of the Poria cocos polysaccharide extract. DETAILED DESCRIPTION

[0030] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1 A composition containing Poria cocos, comprising the following components in parts by weight: 20 parts of Poria cocos polysaccharide extract, 10 parts of Astragalus, 8 parts of Chuanxiong, 10 parts of Bletilla striata, and 15 parts of Licorice; The preparation process of the composition containing Poria cocos comprises the following steps: S1. Weigh astragalus, chuanxiong, bletilla striata and liquorice, crush and sieve to obtain mixed Chinese medicine powder; S2, mixing the mixed Chinese medicine powder with water (the amount of water added is 8 times the mass of the mixed Chinese medicine powder), heating and boiling for 1 hour for the first time, filtering to obtain a first filtrate and a filter residue, mixing the filter residue with water (the amount of water added is 5 times the mass of the filter residue), heating and boiling for a second time for 1 hour, filtering to obtain a second filtrate, combining the first filtrate and the second filtrate, concentrating, and drying to obtain a mixed Chinese medicine extract; S3, mixing the mixed Chinese herbal medicine extract and the Poria cocos polysaccharide extract, adding them into water (the amount of water added is 8 times the mass of the mixed Chinese herbal medicine extract), mixing them evenly, and boiling them at 50° C. for 3 hours to obtain a Poria cocos-containing composition; The extraction process of Poria cocos polysaccharide extract comprises the following steps: A1. Mix 10 g of Poria powder with 120 mL of the first solvent, extract at 40° C. for 3 h, and separate to obtain a first filtrate; A2, mixing the first filtrate with 400 mL of the second solvent, and leaching at 4° C. for 2.5 h to obtain a first leaching solution; A3, mixing the first extract with 80 mL of the third solvent for 30 min, separating to obtain a second extract, adjusting the second extract to neutrality with a 0.5 mol / L hydrochloric acid solution, separating to obtain a second filtrate; A4, dialyzing, centrifuging and drying the second filtrate to obtain a tuckahoe polysaccharide extract; The first solvent is a glyceryl monostearate solution and petroleum ether in a volume ratio of 1:1; The monostearate glyceryl solution is obtained by dissolving monostearate glyceryl in anhydrous ethanol, and the mass fraction of monostearate glyceryl in the monostearate glyceryl solution is 5%; The second solvent includes a sodium hydroxide solution having a concentration of 0.4 mol / L; The third solvent is chloroform and n-butanol in a volume ratio of 4:1.

[0032] Example 2 A composition containing Poria cocos, comprising the following components in parts by weight: 23 parts of Poria cocos polysaccharide extract, 12 parts of Astragalus, 10 parts of Chuanxiong, 12 parts of Bletilla striata, and 18 parts of Licorice; The preparation process of the composition containing Poria cocos comprises the following steps: S1. Weigh astragalus, chuanxiong, bletilla striata and liquorice, crush and sieve to obtain mixed Chinese medicine powder; S2, mixing the mixed Chinese medicine powder with water (the amount of water added is 9 times the mass of the mixed Chinese medicine powder), heating and boiling for the first time for 1.5 hours, filtering to obtain a first filtrate and a filter residue, mixing the filter residue with water (the amount of water added is 5.5 times the mass of the filter residue), heating and boiling for the second time for 1.5 hours, filtering to obtain a second filtrate, combining the first filtrate and the second filtrate, concentrating, and drying to obtain a mixed Chinese medicine extract; S3, mixing the mixed Chinese herbal medicine extract and the Poria cocos polysaccharide extract, adding them into water (the amount of water added is 9 times the mass of the mixed Chinese herbal medicine extract), mixing them evenly, and boiling them at 55° C. for 2.5 hours to obtain a Poria cocos-containing composition; The extraction process of Poria cocos polysaccharide extract comprises the following steps: A1, 10 g of Poria powder was mixed with 140 mL of the first solvent, extracted at 45° C. for 2.5 h, and separated to obtain a first filtrate; A2, mixing the first filtrate with 500 mL of the second solvent, and leaching at 5° C. for 2 h to obtain a first leaching solution; A3, mixing the first extract with 100 mL of the third solvent for 40 min, separating, and obtaining a second extract, adjusting the second extract to neutrality with a hydrochloric acid solution having a concentration of 0.5 mol / L, and separating to obtain a second filtrate; A4, dialyzing, centrifuging and drying the second filtrate to obtain a tuckahoe polysaccharide extract; The first solvent is a glyceryl monostearate solution and petroleum ether in a volume ratio of 1:1; The monostearate glyceryl solution is obtained by dissolving monostearate glyceryl in anhydrous ethanol, and the mass fraction of monostearate glyceryl in the monostearate glyceryl solution is 8%; The second solvent includes a sodium hydroxide solution having a concentration of 0.5 mol / L; The third solvent is chloroform and n-butanol in a volume ratio of 4.5:1.

[0033] Example 3 A composition containing Poria cocos, comprising the following components in parts by weight: 25 parts of Poria cocos polysaccharide extract, 15 parts of Astragalus, 12 parts of Chuanxiong, 14 parts of Bletilla striata, and 20 parts of Licorice; The preparation process of the composition containing Poria cocos comprises the following steps: S1. Weigh astragalus, chuanxiong, bletilla striata and liquorice, crush and sieve to obtain mixed Chinese medicine powder; S2, mixing the mixed Chinese medicine powder with water (the amount of water added is 10 times the mass of the mixed Chinese medicine powder), heating and boiling for 1 hour for the first time, filtering to obtain a first filtrate and a filter residue, mixing the filter residue with water (the amount of water added is 6 times the mass of the filter residue), heating and boiling for a second time for 1 hour, filtering to obtain a second filtrate, combining the first filtrate and the second filtrate, concentrating, and drying to obtain a mixed Chinese medicine extract; S3, mixing the mixed Chinese herbal medicine extract and the Poria cocos polysaccharide extract, adding them into water (the amount of water added is 10 times the mass of the mixed Chinese herbal medicine extract), mixing them evenly, and boiling them at 60° C. for 2 h to obtain a Poria cocos-containing composition; The extraction process of Poria cocos polysaccharide extract comprises the following steps: A1. Mix 10 g of Poria powder with 160 mL of the first solvent, extract at 50° C. for 2 h, and separate to obtain a first filtrate; A2, mixing the first filtrate with 600 mL of the second solvent, and leaching at 6° C. for 1.5 h to obtain a first leaching solution; A3, mixing the first extract with 120 mL of the third solvent for 50 min, separating, and obtaining a second extract, adjusting the second extract to neutrality with a 0.5 mol / L hydrochloric acid solution, and separating to obtain a second filtrate; A4, dialyzing, centrifuging and drying the second filtrate to obtain a tuckahoe polysaccharide extract; The first solvent is a glyceryl monostearate solution and petroleum ether in a volume ratio of 1:1; The monostearate glyceryl solution is obtained by dissolving monostearate glyceryl in anhydrous ethanol, and the mass fraction of monostearate glyceryl in the monostearate glyceryl solution is 10%; The second solvent includes a sodium hydroxide solution having a concentration of 0.6 mol / L; The third solvent is chloroform and n-butanol in a volume ratio of 5:1.

[0034] Example 4 The only difference between this embodiment and embodiment 2 is that, in this embodiment, the first solvent is a glyceryl monostearate solution and petroleum ether in a volume ratio of 1:9.

[0035] Example 5 The only difference between this embodiment and embodiment 2 is that, in this embodiment, the first solvent is a glyceryl monostearate solution and petroleum ether in a volume ratio of 2:3.

[0036] Example 6 The only difference between this embodiment and embodiment 2 is that, in this embodiment, the first solvent is a glyceryl monostearate solution and petroleum ether in a volume ratio of 2:8.

[0037] Example 7 The only difference between this embodiment and embodiment 6 is that in this embodiment, the second solvent further includes a sodium sulfite aqueous solution with a concentration of 0.25 mol / L, wherein the sodium hydroxide solution in the second solvent is 400 mL and the sodium sulfite aqueous solution is 100 mL.

[0038] Example 8 The only difference between this embodiment and embodiment 6 is that in this embodiment, the second solvent further includes a sodium sulfite aqueous solution with a concentration of 0.25 mol / L, wherein the sodium hydroxide solution in the second solvent is 400 mL and the polyethylene glycol aqueous solution is 100 mL.

[0039] Example 9 The only difference between this embodiment and embodiment 6 is that, in this embodiment, the second solvent also includes a sodium sulfite aqueous solution with a concentration of 0.25 mol / L and a polyethylene glycol aqueous solution with a concentration of 0.1 mol / L, wherein the second solvent contains 400 mL of sodium hydroxide solution, 75 mL of sodium sulfite aqueous solution, and 25 mL of polyethylene glycol aqueous solution.

[0040] Example 10 The only difference between this embodiment and embodiment 9 is that, in this embodiment, the sodium sulfite aqueous solution in the second solvent is 15 mL, and the polyethylene glycol aqueous solution is 85 mL.

[0041] Embodiment 11 The only difference between this embodiment and embodiment 9 is that, in this embodiment, the sodium sulfite aqueous solution in the second solvent is 50 mL, and the polyethylene glycol aqueous solution is 50 mL.

[0042] Example 12 The only difference between this embodiment and embodiment 9 is that, in this embodiment, the sodium sulfite aqueous solution in the second solvent is 25 mL, and the polyethylene glycol aqueous solution is 75 mL.

[0043] Comparative Example 1 The only difference between this comparative example and Example 1 is that, in this comparative example, the first solvent only includes 120 mL of glyceryl monostearate solution.

[0044] Comparative Example 2 The only difference between this comparative example and Example 1 is that, in this comparative example, the first solvent includes only 120 mL of petroleum ether.

[0045] Comparative Example 3 The difference between this comparative example and Example 1 is that in this comparative example, the extraction process of the Poria cocos polysaccharide extract comprises the following steps: B1, mixing 10 g of Poria powder with 400 mL of the second solvent, and extracting at 4° C. for 2.5 h to obtain a first extract; B2, mixing the first extract with 80 mL of the third solvent for 30 min, separating, and obtaining a second extract, adjusting the second extract to neutrality with a 0.5 mol / L hydrochloric acid solution, and separating to obtain a second filtrate; B3, dialyzing and centrifuging the second filtrate to obtain a Poria cocos polysaccharide extract; The second solvent includes a sodium hydroxide solution having a concentration of 0.4 mol / L; The third solvent is chloroform and n-butanol in a volume ratio of 4:1.

[0046] The extraction results of the Poria cocos polysaccharide extracts of Examples 1 to 12 and Comparative Examples 1 to 3 are shown in Table 1 below. The yield of Poria cocos polysaccharide extract (%) = weight of Poria cocos polysaccharide extract / weight of Poria cocos powder × 100%: Table 1 Extraction results of Poria cocos polysaccharide extracts of Examples 1 to 12 and Comparative Examples 1 to 3

[0047] As can be seen from Table 1, compared with Comparative Examples 1 to 3, the yield of the Poria cocos polysaccharide extract and the polysaccharide content of Example 1 are significantly improved, indicating that when the first solvent is glyceryl monostearate solution and petroleum ether, the glyceryl monostearate solution and petroleum ether have a synergistic effect and can significantly improve the extraction rate of the Poria cocos polysaccharide extract.

[0048] Compared with Examples 2 and 4, the yield of the Poria cocos polysaccharide extract and the polysaccharide content of Examples 5 to 6 are improved, indicating that when the volume ratio of the monostearate glyceryl solution to petroleum ether is 2:3 to 8, the extraction rate of the Poria cocos polysaccharide extract can be further improved.

[0049] Compared with Examples 6 to 8, the yield of the Poria cocos polysaccharide extract and the polysaccharide content of Examples 9 to 12 were improved, indicating that the extraction rate of the Poria cocos polysaccharide extract can be further improved by using a sodium hydroxide solution, a sodium sulfite aqueous solution and a polyethylene glycol aqueous solution in combination.

[0050] Compared with Examples 9 to 10, the yield of the Poria cocos polysaccharide extract and the polysaccharide content of Examples 11 to 12 are improved, indicating that when the volume ratio of the sodium sulfite aqueous solution and the polyethylene glycol aqueous solution to the volume ratio of the sodium hydroxide solution is 1:4, and the volume ratio of the sodium sulfite aqueous solution to the polyethylene glycol aqueous solution is 0.5:0.5 to 1.5, the extraction rate of the Poria cocos polysaccharide extract can be further improved.

[0051] Application example: Immunomodulatory effect experiment Fifty Kunming mice (5-6 weeks old, 20-24 g), half male and half female, were divided into five groups: blank group, simulation group, experimental group 1, experimental group 2 and experimental group 3, with 10 mice in each group. The experimental time was 14 days.

[0052] Blank group: gavage with 0.9wt% saline, 10mL / d per rat for 14 consecutive days; Simulation group: In the first 4 days and the last 4 days, each mouse was gavaged with 0.9wt% normal saline at a dosage of 10mL / d. On the 5th to 10th day, each mouse was subcutaneously injected with hydrocortisone injection (purchased from Nanyang Tianxing Agriculture Co., Ltd.) at a dosage of 10mL / d. The specification of hydrocortisone injection was 5mg / mL. Experimental group 1: In the first 4 days and the last 4 days, each rat was gavaged with 0.9wt% normal saline at a dosage of 10 mL / d. On the 5th to 10th days, each rat was gavaged with a composition containing Poria cocos (prepared in Example 1) at a dosage of 100 mg / kg / d. Experimental group 2: In the first 4 days and the last 4 days, each rat was gavaged with 0.9wt% normal saline at a dosage of 10 mL / d. On the 5th to 10th days, each rat was gavaged with a composition containing Poria cocos (prepared in Example 2) at a dosage of 100 mg / kg / d. Experimental group 3: In the first 4 days and the last 4 days, each rat was gavaged with 0.9wt% normal saline at a dosage of 10 mL / d. On the 5th to 10th days, each rat was gavaged with a composition containing Poria cocos (prepared in Example 3) at a dosage of 100 mg / kg / d. On the second day after drug withdrawal, eyeballs were removed to collect blood, serum was separated, and the contents of interleukin 2 (IL-2), tumor necrosis factor (TNF-α) and IFN-γ were determined according to the instructions of Mouse IL-2 ELISA kit, Mouse TNF-α ELISA kit and Mouse IFN-γ ELISA kit (all purchased from Youbiai (Shanghai) Biotechnology Co., Ltd.). The results were expressed as mean ± standard error, as shown in Table 2 below; thymus and spleen were taken to determine thymus index and spleen index, as shown in Table 3 below: Table 2 The levels of IL-2, TNF-α and IFN-γ in the serum of mice in each group

[0053]

[0054] Note: Compared with the blank group + model group, (The difference is statistically significant).

[0055] Table 3 Effects of the composition containing Poria cocos on thymus index and spleen index

[0056]

[0057] Note: Compared with the blank group + model group, (The difference is statistically significant).

[0058] As can be seen from Table 2, compared with the blank group and the simulation group, the levels of IL-2, TNF-α and IFN-γ in the mouse serum of Experimental Group 1, Experimental Group 2 and Experimental Group 3 were significantly increased, indicating that the composition containing Poria cocos prepared by Examples 1 to 3 can significantly promote T cells to synthesize more cytokines such as IL-2, TNF-α and IFN-γ, indicating that the composition containing Poria cocos has an immunomodulatory effect.

[0059] As can be seen from Table 3, compared with the simulation group, the thymus index and spleen index of Experimental Group 1, Experimental Group 2 and Experimental Group 3 were increased, indicating that the mice were immunocompromised after injection of hydrocortisone, and the composition containing Poria cocos prepared by Examples 1 to 3 was able to improve the immune function of immunocompromised mice, indicating that the composition containing Poria cocos has an immunomodulatory effect.

[0060] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An extraction process of Poria cocos polysaccharide extract, characterized in that: The following steps are involved: A1, mixing the Poria powder with a first solvent, extracting, and separating to obtain a first filtrate; A2, mixing the first filtrate with a second solvent, extracting, and obtaining a first extract; A3, mixing the first extract and the third solvent, separating, obtaining a second extract, adjusting the pH value of the second extract to neutral, separating, and obtaining a second filtrate; A4, dialyzing, centrifuging and drying the second filtrate to obtain a pachymaran extract; The first solvent is glyceryl monostearate solution and petroleum ether; The second solvent comprises sodium hydroxide solution; The third solvent is chloroform and n-butanol.

2. The extraction process of a tuckahoe polysaccharide extract according to claim 1, characterized in that: The volume ratio of the glyceryl monostearate solution to petroleum ether is 2:3-8.

3. The extraction process of a Poria cocos polysaccharide extract according to claim 1, characterized in that: The second solvent also includes a sodium sulfite aqueous solution and a polyethylene glycol aqueous solution.

4. The extraction process of a Poria cocos polysaccharide extract according to claim 3, characterized in that: The volume ratio of the sodium sulfite aqueous solution and the polyethylene glycol aqueous solution to the sodium hydroxide solution is 1:4; The volume ratio of the sodium sulfite aqueous solution to the polyethylene glycol aqueous solution is 0.5:0.5-1.

5.

5. The extraction process of a Poria cocos polysaccharide extract according to claim 3, characterized in that: The concentration of the sodium hydroxide solution is 0.4-0.6 mol / L; The glyceryl monostearate solution is obtained by dissolving glyceryl monostearate in anhydrous ethanol; The mass fraction of glyceryl monostearate in the glyceryl monostearate solution is 5% to 10%.

6. The extraction process of a tuckahoe polysaccharide extract according to claim 1, characterized in that: The mass volume ratio of the Poria powder and the first solvent is 10g:120-160mL; The mass volume ratio of the Poria powder and the second solvent is 10g:400-600mL; The mass volume ratio of the Poria powder and the third solvent is 10g:80-120mL; The volume ratio of chloroform to n-butanol is 4-5:

1.

7. A composition containing Poria cocos, characterized in that: The invention relates to a Poria cocos polysaccharide extract obtained by extracting the Poria cocos polysaccharide extract using the extraction process of any one of claims 1 to 6.

8. The composition containing Poria cocos according to claim 7, characterized in that: The composition comprises the following components in parts by weight: 20-25 parts of Poria cocos polysaccharide extract, 10-15 parts of Astragalus, 8-12 parts of Chuanxiong, 10-14 parts of Bletilla striata, and 15-20 parts of Licorice.

9. The process for preparing a composition containing Poria cocos according to any one of claims 7 to 8, characterized in that: The following steps are involved: S1. Weigh astragalus, chuanxiong, bletilla striata and liquorice, crush and sieve to obtain mixed Chinese medicine powder; S2, mixing the mixed Chinese medicine powder with water, heating and boiling for the first time, filtering to obtain a first filtrate and a filter residue, mixing the filter residue with water, heating and boiling for the second time, filtering to obtain a second filtrate, combining the first filtrate and the second filtrate, concentrating, and drying to obtain a mixed Chinese medicine extract; S3, mixing the mixed Chinese medicinal extract and the Poria cocos polysaccharide extract, adding the mixture into water, mixing evenly, and boiling to obtain the Poria cocos-containing composition.

10. Use of the Poria cocos-containing composition according to any one of claims 7 to 8 or the Poria cocos-containing composition prepared by the preparation process according to claim 9 in immunomodulation.

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